NCBI accession DQ898156; Daucus carota chloroplast, complete genome.

Resource Type: 
Analysis
Name: 
NCBI accession DQ898156; Daucus carota chloroplast, complete genome.
Description: 

Assembly deposited as NCBI GenBank accession DQ898156

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DNA was sheared by nebulization, size fractionated to 4–6 kb, linker ligated and cloned into pHOS2, a TIGR medium copy vector. A total of 1231 high quality reads with an average length of 808 bases was generated during the random (1126 reads) and closure (105 reads) phases of sequencing. Sequences were assembled using TIGR assembler and scaffolded using Bambus. Sequence finishing included directed PCR to span gaps and directed primer walking of clones to cover the entire genome and to complete regions of low depth of coverage.

Publication: 
Ruhlman T, Lee SB, Jansen RK, Hostetler JB, Tallon LJ, Town CD, Daniell H. Complete plastid genome sequence of Daucus carota: implications for biotechnology and phylogeny of angiosperms.. BMC genomics. 2006 Aug 31; 7:222.
Cross Reference: 
NCBI Nuccore
NCBITaxon
Program, Pipeline, Workflow or Method Name: 
TIGR assembler + Bambus
Program Version: 
DQ898156
Date Performed: 
Thursday, August 10, 2006 - 19:00
Data Source: 
Source Name
: NCBI accession DQ898156
Source Version
: DQ898156.1
Organism: 
NameCommon NameComment
Daucus carota
Core Data: 
plastid_assembly