1995 Celery RFLP+RAPD

A F₂ population of two celery cultivated types (Apium graveolens L. var. rapaceum A112 celeriac, and A. graveolens L. var. secalinum A143 annual smallage) was used to construct a linkage map consisting of 29 RFLP (restriction fragment length polymorphism), 100 RAPD (random amplified polymorphic DNA), four isozyme, one disease resistance, and one growth habit markers. The map contains 11 major groups and 9 small groups and has a total length of 803 cM with an average distance of 6.4 cM between two adjacent loci. Ten percent of the RAPDs segregated as codominant markers and their allelic homologies were tested by Southern hybridization. One-quarter of the dominant RAPDs were linked in repulsion phase, whereas the majority of them were linked to either codominant or dominant markers in coupling phase. About 10% of the markers showed significant segregation distortion. The detectable level of duplications in the celery genome was relatively low.

Accession A112 was the authors' designation for PI 169001, and accession A143 was the authors' designation for PI 257228. Note that in GRIN PI 257228 is listed as A. graveolens L. var. rapaceum. This F₂ population is designated as PI 169001 x PI 257228 in CarrotOmics.