Trachyspermum ammi (L.) sprague fruits is commonly called ajowan belongs to the family ‘Apiaceae’. It is an annual herbaceous plant bearing feathery leaves and white flowers grow in compound umbels. It is indigenous to southern India and it is cultivated in various areas such as Europe, Egypt, and Pakistan, Afghanistan and in Iran. Trachyspermum ammi’s fruits yielded brownish essential oil, with 35% to 60% thymol as the major constituent. The seeds of Trachyspermum ammi have several therapeutic effects, including diuretic, antivomiting, analgesic, antiasthma, and antidyspnea effects. The de novo transcriptome analysis of ajowan flowers in this study provides an important resource for discovery of functional genes related to the formation of major components in the essential oil from T. ammi seeds. Contributors: Soltani-Howyzeh,M., Sadat-Noori,S.A., Shariati,V. and Amiripour,M.Department of Agronomy and Plant Breeding, Aboureihan Campus, University of Tehran, Emam Reza blvd., Pakdasht, Tehran, 3391653755, Iran