Daucus carota (carrot) is one of the few plant species that synthesize and accumulate carotenoids in the storage root that grows in darkness. Contrary to other plants, light inhibits secondary root growth and carotenoid accumulation, suggesting the existence of new mechanisms repressed by light that regulate both processes. In order to identify genes induced by dark and repressed by light that regulate carotenoid synthesis and carrot root development, we performed RNA-Seq analysis from dark and light-grown carrot roots.
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| Instituto de Nutricion y Tecnologia de los Alimentos - Universidad de Chile | University |
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Plants of commercially acquired carrot (Daucus carota L.) cultivar Nantaise were harvested 8 weeks after sowing. RNA was sequenced by OMIC Solution (Santiago, Chile) using the Ion Torrent PGM technology (chip P1.1.17) with IonXpress RNA kit (Thermo Fisher Scientific) after library profile analysis using an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Adapter sequences were removed, and a de novo transcriptome assembly was carried out following CLC Genomics Workbench default parameters including a quimera analysis. The assembly contains 63,164 contigs, from which 18,488 genes are differentially expressed (DEG) between the two experimental conditions light and dark. |
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Carrot roots cultivated in dark (R/D) or in light (R/L). | |
Carrot roots cultivated in dark (R/D) or in light (R/L). | |
Carrot roots cultivated in dark (R/D) or in light (R/L). |
| NCBI BioProject | |
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