Anther and ovule in vitro culture in carrot (Daucus carota L.)

Resource Type: 
Publication
Publication Type: 
Journal Article
Title: 
Anther and ovule in vitro culture in carrot (Daucus carota L.)
Authors: 
Domblides AS
Series Name: 
Acta horticulturae
Issue: 
1153
Page Numbers: 
55-60
Publication Year: 
2017
Publication Date: 
2017
Cross Reference: 
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The pub, Anther and ovule in vitro culture in carrot (Daucus carota L.), is a part of pub, International Symposium on Carrot and Other Apiaceae.
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Citation: 
Domblides AS. Anther and ovule in vitro culture in carrot (Daucus carota L.). Acta horticulturae. 2017; (1153)55-60.
Abstract: 
The current biotechnological approaches enable to develop the method for production the double haploid lines through anther culture and culture of unpollinated ovules in vitro. As it was shown in carrot there are two methods which can be used to obtain such lines. These techniques were utilized in two carrot cultivars Nantes 4 and NIIOCH336. In general, 450 ovules and 223 anthers were placed on medium with 2,4-D. After 5-6 weeks the active callus formation can be noticed on ovules. All calluses and embryoids obtained were then transferred to the fresh medium with kinetin for plant regeneration. Total number of ovules with gynogenic structures was 36% with callus and 2.1% with embryoids comparing with anther culture where yield of callusing anthers reached 22.2%.
Publication Model: 
[electronic resource].
Language Abbr: 
eng
Keywords: 
  • 2,4-D
  • Daucus carota
  • anther culture
  • anthers
  • callus
  • callus formation
  • carrots
  • cultivars
  • doubled haploids
  • kinetin
  • ovules
Notes: 
p. 55-60.