Resource Type:
Publication
Publication Type:
Journal Article
Title:
Some AFLP amplicons are highly conserved DNA sequences mapping to the same linkage groups in two F₂ populations of carrot
Series Name:
Genetics and Molecular Biology
Journal Abbreviation:
Genet. Mol. Biol.
Volume:
25
Issue:
2
Page Numbers:
195-201
Publication Year:
2002
DOI:
10.1590/S1415-47572002000200013
ISSN:
1415-4757
References:
Citation:
Santos, Carlos A.F. and Simon, Philipp W. Some AFLP amplicons are highly conserved DNA sequences mapping to the same linkage groups in two F₂ populations of carrot. Genet. Mol. Biol. 2002, vol.25, n.2, pp.195-201
Abstract:
Amplified fragment length polymorphism (AFLP) is a fast and reliable tool to generate a large number of DNA markers. In two unrelated F₂ populations of carrot (Daucus carota L.), Brasilia x HCM and B493 x QAL (wild carrot), it was hypothesized that DNA 1) digested with the same restriction endonuclease enzymes and amplified with the same primer combination and 2) sharing the same position in polyacrylamide gels should be conserved sequences. To test this hypothesis AFLP fragments from polyacrylamide gels were eluted, reamplified, separated in agarose gels, purified, cloned and sequenced. Among thirty-one paired fragments from each F₂ population, twenty-six had identity greater than 91% and five presented identity of 24% to 44%. Among the twenty-six conserved AFLPs only one mapped to different linkage groups in the two populations while four of the five less-conserved bands mapped to different linkage groups. Of eight SCAR (sequence characterized amplified regions) primers tested, one conserved AFLP resulted in co-dominant markers in both populations. Screening among 14 carrot inbreds or cultivars with three AFLP-SCAR primers revealed clear and polymorphic PCR products, with similar molecular sizes on agarose gels. The development of co-dominant markers based on conserved AFLP fragments will be useful to detect seed mixtures among hybrids, to improve and to merge linkage maps and to study diversity and phylogenetic relationships.
Language:
english
Language Abbr:
eng
Journal Country:
Brasil