T2T genomes of carrot and Alternaria dauci and their utility for understanding host-pathogen interactions during carrot leaf blight disease.

Resource Type: 
Publication
Publication Type: 
Journal Article
Title: 
T2T genomes of carrot and Alternaria dauci and their utility for understanding host-pathogen interactions during carrot leaf blight disease.
Authors: 
Liu W, Xu S, Ou C, Liu X, Zhuang F, Deng XW
Series Name: 
The Plant journal : for cell and molecular biology
Journal Abbreviation: 
Plant J
Publication Year: 
2024
Publication Date: 
2024 Oct 07
DOI: 
10.1111/tpj.17049
ISSN: 
1365-313X
EISSN: 
1365-313X
Cross Reference: 
PMIDLoading content
Citation: 
Liu W, Xu S, Ou C, Liu X, Zhuang F, Deng XW. T2T genomes of carrot and Alternaria dauci and their utility for understanding host-pathogen interactions during carrot leaf blight disease.. The Plant journal : for cell and molecular biology. 2024 Oct 07.
Abstract: 

Carrot (Daucus carota) is one of the most popular and nutritious vegetable crops worldwide. However, significant yield losses occur every year due to leaf blight, a disease caused by a fungal pathogen (Alternaria dauci). Past research on resistance to leaf blight disease in carrots has been slow because of the low-quality genome assemblies of both carrot and the pathogen. Here, we report the greatly improved assemblies and annotations of telomere-to-telomere (T2T) reference genomes of carrot DH13M14 (451.04 Mb) and A. dauci A2016 (34.91 Mb). Compared with the previous carrot genome versions, our assembly featured notable improvements in genome size, continuity, and completeness of centromeres and telomeres. In addition, we generated a time course transcriptomic atlas during the infection of carrots by A. dauci and captured their dynamic gene expression reprogramming during the interaction process. During infection, A. dauci genes encoding effectors and enzymes responsible for the degradation of plant cell wall components, e.g., cellulose and pectin, were identified, which appeared to increase pathogenic ability through upregulation. In carrot, the coordinated gene expression of components of pattern- and effector-triggered immunity (PTI and ETI) in response to A. dauci attack was characterized. The biosynthesis or signal transduction of plant hormones, including JA, SA, and ethylene, was also involved in the carrot response to A. dauci. This work provides a foundation for understanding A. dauci pathogenic progression and carrot defense mechanisms to improve carrot resistance to leaf blight disease. The Carrot Database (CDB) developed also provides a useful resource for the carrot community.

Publication Model: 
Print-Electronic
Language: 
English
Language Abbr: 
eng
Journal Country: 
England