- Iorizzo M, Ellison S, Senalik D, Zeng P, Satapoomin P, Huang J, Bowman M, Iovene M, Sanseverino W, Cavagnaro P, Yildiz M, Macko-Podgórni A, Moranska E, Grzebelus E, Grzebelus D, Ashrafi H, Zheng Z, Cheng S, Spooner D, Van Deynze A, Simon P. A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.. Nature genetics. 2016 06; 48(6):657-66.
- Osuna-Cruz CM, Paytuvi-Gallart A, Di Donato A, Sundesha V, Andolfo G, Aiese Cigliano R, Sanseverino W, Ercolano MR. PRGdb 3.0: a comprehensive platform for prediction and analysis of plant disease resistance genes.. Nucleic acids research. 2018 01 04; 46(D1):D1197-D1201.
PRGdb |
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The mRNA, DCAR_000615, is a part of gene, DCAR_000615. |
Name | Description |
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An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |
Transcript Name | Identifier | Type | Location |
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DCAR_000615 | DCAR_000615.mRNA | mRNA | DCARv2_Chr1:890931..910708 |
ATGGAGTCCAATGGCTTAAATGAAGCTCTTCGTGATGTTAATGACCACCC
AGGTAACAACTTAAATGGTTCAATTCAAGGTATAATTCCAGTGTTTATTT
TGCTACTGTTAGTAATTGAATATAATAAAATTTGAAAATTTATTTATTAA
TTCAGTCCAAATACAAGTACAAGCACACACTGTTTTAAATTAAAGTTCCA
ATCTAATATGGAAGAAGATAATTTGGTTGCAAGCTAGTGTCGTGCCAATT
ATGACTAGTTAAGCACACAAATGCTCCCCATGGTTCTAATATGGAGAAAA
TTATTTGTCATTGCAAATATAGGTTATTCTAACTGTAGCTGACTTTGTTC
CCAAGTTCGACACAAAGACATGCTAAAATAGTCTTTCTCTTGAAATATCA
CTTTAGATCACAACTTTTAGACTCTAACTAGACTAAAGCTTCTGCTGGTA
TGCAAAATTTGATAAGTTTTTTTCATAATAAGACTGTATGCTTACTTTGT
GTTTTAATACTTTATTTTTGGTGGATCATAGGACCATTGACTGATACACT
TAATACTGGAAACAAGTCCCGTGATCCTTTTAACTCGATGGATGGATGTT
TTGCTGATGTTTCTGCTGCAGAGATTTCTGAGTTGTTGAAAGTGAACAGT
ATAGAGGTAACTATGATAGCTATTTCTCCGTTATGTTTTGAGATAATGCT
GTATGAGGGGATTCTCGTAGCACGTAATTTGGTTTTGCAGTTTTTCCTTA
AAGCATATGTGCTGGTACTTCATCGAACACTAACAGCCTTTCAAATGTAC
TGTGAACCACTATACCGATTATACTGCATCGTAACTGCTGTCCTGTCATG
ATTTTATAATTTAAAAAACAGCCCATTATGACTAAATATTATTTATTATA
ACTAATATTAGTAAGTATTAGTATTATTACTACTACCAAGATAGAAATAC
ATGTCTTGGATTGATAATAATAGATTAAACAAATGATATATTGTACACCT
ATGAGAGGCAGTCCTAGTTAGTAGTAAGTAATGATGTGCTTTAAATGCTT
CAGGAGCATTCACAATGACCAGACTGCACCAGTCCATTCATAAATCTTTG
CACCAAACCCACACTGATTAGGAAAGTGTGCGGTGCACATTGTCCGCTTT
ATGCCTTTTGCCCTGTCTGTGTTCTTTTGGTCGAATTAGTCATGTCTACA
TATGTTTGAATTGTAGTATTATCTCCTTGTTTGCTTAATTCACACCTACA
CGTGTGCTATGTTTAATAAGCTAATTATGCATTATTCTTTTGACAACTTT
TATGTGCATTGTGCAGAATGCGTCTACTCGGTCACTTCTCAGTGTTGCTA
AGAAGATTTTGGATGAAAATATAGAAAGAAAAAATGGAGATATTCCCAAT
GTATGAAACTTAATATTGCACCCAGGACTGTCATAGTGTGGCACCCAGAA
ATATTTTATTTAAGTTATGTAGCTAGTTCCCTTCGTTGTGTTTTGTATTT
TAACTAAAACTATGTTTTCAGCGTGTAGCCTCTTTATTAAAAGTTGTAAT
CCAAGAGATGGACCAAAGGTTTTTTAGGCAAGCTGAAAACTTGAGAAAGG
TATACACAGTATTTTGTAATATCACAGTTTTGCTTATGGTCATATTAAAT
TTTCCTGTGTATAATTATATTAATAGCTTAGTACTTGACTGCAGCAAAAG
TGTACTTACAAGTCTCGTGAGGAGAAGCTTCAAACAAAGATAAGAGTACT
AGAAACACTTGCAACAGGGACCACAGAAGAAAATGAGGTGCTATTTAAAG
GGAATGTGCACATAGACTAAATATAGTATTATTTATTCTATTTATTTTGT
TTTTTTTTTGAATTTACCGTCTGGTTGTATGTGTAGGTTGTCCTGAATCA
GCTTCAGAAAACAAAGGTGCTTTTCTTGTCTACGAGTCTTACTTTTATTG
TCCTTCATTCAAGACACATTCCTTTGATTTTGTTAGAAAGATTAAATTGC
TAAACTAGTCCAATTTCGTGTTGCTTGGTTCCTTTTAAGACTATATAAAC
AACACATGTATGCAAAGACTATCTTTAATCCTCAATTCGACTTGATCTGT
TTCTCCAAAGTAGTATGCTGGCTGCTTTCCAACAATGTACTTCTTTTAAT
ACCTCATATTACTAATTGAGCCTGAGTCATGACTTGTAAAGAAGTCACCA
TGTAACTACTCAATTTTTACTGGTTACGGAGTCCAGATTAGAGTATTTGC
AAATGCCAAGGTCAATTTTATGAGTTCTTTGACTACAATATTTCCACATC
ACTTTAATGCAAAGGAGCAGATTTCGGGCCTTTTTGATATGAATGTGTAT
CACCATAACCAAGAAAATCTGTAGTATTACTCTTTTTTGTAATATTAACT
TCAATGGTGTCTTCTCTCCCTGATTAATTGTGGTGAGGTCCAAGCATATT
CAGTTCATCAGCATAAAATAGAATCGGAGTACAAAAAAGATGCTTGTTTT
CTGCAAAGTTGGATATCATGGGGGGATGTTGCTTGTTTACTATTTGTCAT
AGAGTAATCTTTTTACTTTGGGCATATTTAAATCTTAGCTCATCATCTTG
TTTATCTTAGCTCATTCAATTGCACGTTGCATGCACAGATGGAGAAAATG
GAAATAGAGGAGAAAAAAAAACTTGAAGAAGAAGATGCAGTTAGGCTACT
GAAGCTCAAAGAATCTAGTGATAATCATGTCATAACACTTCAGGAAGAAC
TAGATGTGACAAAAAGGACATATGAGAAACATTGCTTTCAACTGGAAAGT
CAGGCGAAAGAAGCTAAAGTTGAGTTACAGAATAAGATAACTGAGCTTGA
GTGTCTTTTAGCTGATTCAAGGAAGAAGGTGGAAGAGCATCAGGCCTCGA
CACAATCAAAATATAGGAGATGGAAAAGGAAAGAACTTGGATATAAGAGA
TTCCTAGATTTGAACTCTGGATCCCTGCAGGTTTTTGATAGATTAGTATA
TCTGGATTATAATTATGTAAATCTGTGTATTTCATGATCTTATAATATGT
TATGCAAACTACTCAGGATTTAAGGATGCTTTCCAACTCCTTAAAGCAAG
AAATTCTGAAGATACGAAATTTTCATATGGAGGAACTCAATGACTTAGGT
ACATTCGATAGCCCAACAGTATGTTGAATCAATATATAGCCATTGTACTT
TCCGGCTTAGTTTCTCATCTTCATCTTCTTAACAGGATTGAAGCTTACAA
GCCTGGTTGAGGCTGCTCAGAATTATCATGTGGTTCTAGAAGAAAACAGG
AAGTTGTACAATGAAGTCCAAGATTTGAAAGGTATTTTGTTTTTCCTTAA
TTATCATGGTTGAGTTGTTTATGCTCTCAATAATTTTATCTAATCTAAGC
TACCTGAATCTTTATTTGGCAGGAAATATTAGAGTTTATTGTAGGATCAG
ACCATTTCTTTCGGGACAAAGTCGGAAACTAACCACCATTGAGTACATGG
GCGAGAATGGTGATTTGGTGGTAAAAAATCCATCAAAACTGGGAAAGACC
AGTCACAGGCTTTTCAAGTTCAATAAAGTCTTTGGTCCTACGGCAAGTCA
AGGTATCAACTAGCTGAAGAACATATGATTTCAGTTGTTATTAGTTCAAT
CCAGTTGTTGGTGTTAGTTTTACTTCTGAATTTCATAAGTTATAATATAT
CTTTGCAGAGGAGGTTTTTCTAGACACCCAACAATTAATTCGGTCGGTTC
TGGATGGATACAACGTTTGTATATTTGCATACGGCCAAACAGGTTCTGGA
AAGACTTACACAATGGTATGATACAATTATGATTCACATGTTGACAAGTT
TTTCTTTTAATAATTTTTATGCGTGCAATATATTTGCTCCATGTGTATGC
TATAATTTGTTGTTTTTTTGCAGACAGGACCAAATTTATCATCCAAAGTG
GACTGGGGAGTCAACTATCGTGCCCTGAATGACCTCTTTCATCTGTCACA
GAGCAGAAAAAGTTCAATCACCTACGATGTTAGTGTCCAGATGGTCGAGA
TATACAATGAACAAGTGCGCGATTTATTAACGGGAAACAGTCAACAGAAA
AAATATCCTTTACCTTCGCATTTTGAATTAGCAATAGTTCAGTTTTACTC
GTATCTTCCATTTTATTGCTGTTTAGCATCAAATAGACGCAGAAGTTGCG
ATTCTAGTTTTCTTTAACTTGCAACACTCTAGGAATTTGGAATTCTACTC
AACCAAATGGTTTGGCGGTGCCTGATGCTAGCATGCACCCCGTGAAATCA
ACGTCGGATGTCTTGGAACTAATGAATATTGGATTAATGAATAGGGCTGT
TGGTGCTACAGCCCTAAATGAAAGAAGTAGCCGATCTCATAGGTACATTT
ATTTGCAACCCGATATTCTTTTAAGTTAAACCTGATATTTTTCGAGATCA
TTATACAGTTTTTGCTTTCTCAAAATTTATGTTGTATCCCTTTGTTGCAG
TGTTCTTAGTGTTCATGTTCGAGGTATGGATTTGAAGACCAATACAATTC
TGCGTGGCAATTTGCATCTGGTAGATCTTGCTGGGAGTGAAAGAGTAGAT
CGCTCTGAAGCCACTGGGGACAGGCTACGTGAGGCGCAACATATAAATAA
ATCATTATCTGCCCTAGGTGATGTGATATTTGCTTTGGCACAAAAAAGTC
CACACGTGCCATACCGAAACAGCAAATTAACTCAAGTTCTTCAAAGCTCT
TTGGGTAATTATGCATTGATTCTTATTTATGATGAACATAGAAAGAATCG
GGTTATTGCTCTTTATAAATGTGATTTAACGAAACCATAAAGTATGGTAG
GTCAATATGTTTGTTAATTGCTATATGTCTAACTTGCTTTTTGCATTATG
TATTTCCTAGGTGGCCAGGCAAAGACACTTATGTTTGTACAGCTCAATCC
AGATGTTGAATCCTATGCTGAAACCATCAGTACACTGAAGTTTGCTGAGA
GAGTGTCAGGTGTTGAGTTAGGTGCAGCTCAGAGCAACAAAGAGGGAAGG
GGTGTAAGAGAACTTATGGAGCAGGTTAGATACTTTGCTATTTTCTGCGA
CAGTTTTTATTTTGAATATCTTATTTCTTGAAGAAAATACAAGCTAGAAA
ACAAGTGAGCACTTTAACTTGCTTCCCGAAAATCTCATGGGGATGGTCAA
AATTTTTCTAGCAGTCAGTGTTGTTTAAGGCGCACAATTTAGAAAGGCGC
AAGACCCAGATTTGAGGCGCAACTCGCGAAGCGCCGTTAATTGCGCGCCT
TTTGGCCAAAAAACAGTTACTCACATATCTAACAAATAAGTAATACTCCG
TACTAAACATATTCTTGACATTCAAACATAACAAAGAGCATAAATAACAT
GATAAAAGTGCCAATAATAAAGCCTTAATCAATAAGGTTCTCTATTTCAA
AAGCTGTGGTGTTATGTAGCTCAATCTTCTGCCCTTCAAATTCTCTTCTC
TTACTCCATCATCTTCAACTAGTAGACTTGATGTTGAGTGAATAAAAGCT
GAGCGGCTGAGATCTGAGAGATGAAACTCTAAAGTGTGTTTTTTCTCCTT
TTAACCTTCTTTTTATTTTATGAGCGCCTTTTTGCAATTCTTAGCACTTT
TAGCGCCTTTTAGTGCCTTTTGGATGATTTCAGAATCGCTCTAAAGCGCA
CATTTTTGAATCGCAGATCTTGCGCATTATCGCGAAATTCAGTTTGCGTT
GTGTTCAATCGAATTGTTGAACACACGGTTTTGGACTTGGAAAGCATTTT
ACTATCCAGATTCAATCATGTCGGAAGACTGAAAAACAAAAACATACAAC
TAGTATTAAATCTTTCAGCTAGTTTTAATTTGAAGATGTAAGAAAATTGA
CTAGTGTGGAAAGCACTTAATTTTATGTTTGAGAGATGTGGGAGAGCTAA
TGGTGGCTTAGTGATGGGTGTCAATCTTGATGAGGTCCAAATCCCTCATA
TTTTCGAGACCCTAAGAGTACACATTCCAAACCCATCAAAGGAGCAGTCT
GCTGGAATTGTCCAAGAATTTAATCTGGGCTTTATTGCTTTCCTTTGCGT
GCTCAAGTAATCATGAAATGAAACCTCTGCATGTCATTCCAAAATCCTAA
TACCTCTCGTACCGTCAAAAAGTTAATATATCCCCCTTCGTGAATGCTAA
CTTTATCAGTTCATGATAATTAACTTAAATAAGATTTTAGGGCTACAATG
TTAGTTGATTGATCTACGGTCTACAGACATGTGTTCTCACTAAATATGTC
TGGCAGTTTCTTTGCATAGGGTTCTGAATATTAATTTTTTATCCTTTTCT
TGTTGATGTTTATAGGTTGCAATTTTGAAGGACGCAGTTGCAAGTAAGGA
TGAGGAGATTAGCCTTCTACTGGCTAAGACTAACTTAAGTGCTGAGAAAC
GTGCTTTGAGCTTACCTAGTTATGGGTCTTCCTCTCCCAGAAGACACTCC
ATAGGCAGTCCAAGGTCAAACAGAAGAATATCAGGTTGTAGAAGTCCCAA
AGTTTTTGGAAAAGCAGCTTCAGATATTGAAAAGAGTTCAGAGGACAGTG
ATAAGCATTCTGAAGCTGAATCCCGGCTTTCAGATGACTTGAGACACAAC
AAGGAGTTTTCCCAGCTATCACGACCTGCTGCTATAGACGCTACTCTAGG
TCTTACTGAAGAAGTTGTATCAAGAATACCTTCAGTACATAAACGTCATA
ATTCTAATGATGATTTTGAACTCCTTGGATTTGGGGATGCAGAGTCCGAG
GGGAGACTAAGTGATATATCAGATGGTGTTCTCTCCATGGCAACAGAAAC
TGATGGTTCAATTGCCAGTATTGTAGAATATACTCTATTTCCAGAAGCTT
CACTATCAACATCGGAGGTTATAACAGAAAGCACAATACCAGCAGCTGAG
AGCACAAAGCCACCGGTGGAGAAGTAAGCATTACATATAATTGATCTTAT
TACTTTCGGTAATTTGTCTGATATAGTTTAGTGAACCAACTGAAAACTCT
TGTGCCCTTTACCTGCCCTCTCATCTTTGTAAAATGTCAAATACTTAAAT
GAATTACTTATAATATTCAATTCAAATTTAGGTATATTAAGTGCTTTATC
TACTGTCCATAAGTTATGCATCTTCTTTTTAAAATTTTTATAATGCTTGA
ATTCCAGGTCCAGTGTGTCAGCTAGAGTTCCAAGACCTGCGCAAAAGCCA
TCACAAGGATCTTCTCGCATGTCATCATCTAGAAGCTCCACAAAAGTCTT
GCCAAGTATGCATAACTATTACTTTGAAATTCCAGTCTATATATTAAGTT
ATATAACATTTTATAATTCAAGAGCAAATCTTCTAATACTTTTTACAGAC
TTGTTGCCAGCAATAAACAATTATCTAAATATCATTCCTTCCCCCTTCTG
TCTGTTATTCAGGTTCTAAAAAACCAGCAACGGGCAGTTCCTCTATAGTC
AAGTCAAACAGCGCATCCTCTTTACTCAAGTCCGGCAGGCGGCGTTTATG
AAGCAAGATAGGCGTTTGAGGGCCGAGTAGCTCCCTGTGTCGGCTTTCAG
TCCTCCTGAAAGCATGAAAACATCGGTGAAACTTATGTCAAATTAACATT
GGTAGTGTAGTACTATTACTAGCCAAGTGTCTGGAAATGCAAGTGAAAAG
TAGATTATTTATTGTATATCAAGGTGGTTGTAGTTGCCAATTTAGACATC
ATTTTCTCTTTGTAGACTATATACAGCAAGCTTAGAAGATGTTGAATTGC
TGGTGCAATGTGGACACAGAGGTTTGTTATTATATTTATTATTAAAAAAT
CCTTACATGTGTTTTTTAAAAGATTTATTTGTAAAATATGCAACCAACTT
CCAGGCATCTTTTACTTTCATCCAGGTAACTCTGTTTTAACGAAATAACT
CTATTGGGCATGGGCCCGGCTAATTATTGGATTTCAGTGTAGCTCCCAAA
TTACTGAATTGGGCCGTGCTAATAGAGGCCATGCATGTAAAAGTATGGAC
CTGACCCGACCCAGCAGAACTTCCATGCTGGATAGAACGAGTCGCGGGAA
GATTGAATCGACAGACCCAGACCTCTTATGCAAGATAGTAATTAATACTC
CCTCCGTTTCAAATTAGATTTCCACTTTAAAAAAATCACACAATTTAAAA
AAGGGAAATCTATAAAACTACCTACCTTTTCTTTTATTGTTTTCAAAAAT
ACTATCTTCCAGAATTATTTTTAAAAATACCTTTTCATAAATTTTTTTTT
TTAAAAATACTGTTTGCAACTTTTGCAACCTCATTTGCAACTACAGGCGG
CGCCAGCCGTGTTGCAACCTGATTTCAAGTTCCAGTTTTCAAATGTCATT
TTCGACCTCATTTTCGGAATCGATATATATATATATATATATGCATATGG
TTGTATTCAGTTGCATATGGTTGCATTCAGTTGATTCTATTGTAATCTAA
TGGCCCCGCCAGGGGCACACCATACATATGTTGTTACTTACAGTTTTCAG
TTGCATTTAGTTGCAACTGAGGTTGCAAAAGTTGCAACTGCAGTTGCAAC
CTCATTTGCAACTTTTGCAACCTCATTTACAACTATATATAATTTTCAGT
TGCATTTAGTTGCAACTGTGGTTGCAAATGAAGTTGCAACAACTTTAGTT
GCAACTTCATTTGCAACCTCATTTGCAACTTTTACAACCTCATTTGCAAC
TTTTGCAACTTACAGTTTTCAGTTGAACTAGTTGCAATTGCAGTTGCAAC
AGTTGCAACTTTCCATTTTTATTTGCAACTTTTGCAACCTTATTTGCAAC
TTTTGCAAACTCATTTGCAACTTCATTTGCAAATGAGGTTGCAAAAGTTG
CAAATCGTATTTTTGAAAAAAAAAAATTTATGAAAAGGTATTTTTAAAAA
CAATGAAAAAGATGGTAGTATTTTTAAAAAAATAAATTTACAGATGGGTA
TTTTTAAAAAGATCTCTTTAAAAAAAGTGGTTGTTCACAAATTAATTGCA
TCAAAATATGTGGAGTGAGATTGATCTAAGAAATATAAATAAAAGATATG
CGGAGTAGAATTGATTTTGGAAATATAATTTTGCATTGAAAGTTGAATTG
GACAAGTAATTTGAAACAATTTTTTTTTTGAAAGTGAATATGTAAATTGA
AACGGAGGGAATATTATATATATAATTTCAATAAAAATTATAAAAATTAG
GTAAAAAAAATTAATTCTAATTTTTACTTTCTATAATTTTAATCAGAGCC
ATGTTGATTATATTTGGCTAACTTCTAAATACTCCTATCGATTATATTCA
GCCGGTCTCTGGTAATTTGGAGGAGGATTTTGCATCGTCTATTTTCATAT
TTATATAATACTAGTTTTTAACTCGTGCGAAGCACGGGCGGGTATATAGT
TCGTAATTTAGTATTTATAATTTAAATTTTAACATCATTTTATTAGTATT
TTCGTATTAATGGATTGAATTTTAATTAAATTATAATATTCAACTGACTA
TATTTTCTCCCGATTACTTAAAAATGGACAAACCCAAATATATATATATA
TATATATATATATATATATATTAGAATTTTAGTACATTTAATCAGAATTT
AGTACACATAGATTTAAAAATCAGAAAATTAGAAAATTCTAATCTTTTTC
AAACATAGTCGATCGTGTAATACCTTTGAAATATTCAGTAATCTAATCAA
TCGAATTTTAACGTAATTTTGTAATCTTAGTTTTTTTTTACAACAATAAT
TTTTAAGATTAAAGTTAGAAAGGATTATGTAATGGTTATATTGAAATAGA
ATACGTTAAAGTTAAAAAGAGTTATACGAAGTTCTTGTTAAAAAAAGATA
TTCAAAATTGTATATTTATAATTTTATTTATAACATCATTATAATTTTTT
TAATAAAATATTATATGATAATGTATTGTTAGGAGTGTTTTTAGTATTTG
AAACTGTTTATAATACTAATAGACTCCACATTTATAGACTTGTAGTACCA
AAAGAAGAGTACCAAACCAAAAAAATATAACTAAAACTTTGTACTACAAA
TTATACAAATTATACCCATGTTGGTTTATTATAGTATAGTATAGATATTT
TATATATAATAATTTGAAACAATAATAATATATTATTTTTAGATGATATT
CAAACAATGTAAATAGTATCACTGAGACAATATATGCGTTCAACAAATTT
ACGAAAAAGATATCTTTTAAATTTGATTTAATTATATATATTTACAATTC
TAACGGAAAAAGACACATAAATAAATAAAAGTTACATATATGTATATATA
TTTTCAATTTGTTGATATTGAATTTTATTTGTTGAAGTTTAAAAAATTCG
TAGTGAAATTGCATTTCTCTTGTAATATAAAATATTACATGATAAAAAAC
AATATATAATTTTTAAAATATAAATAATCATTATACTATTTTGCCCCCTA
AGAATGATTTATGGATTTAGCAAATTTTATATAATATTATTGGACATAAA
TCATCAAAAAACTGTTGCGTTCTTAATTGTTGCACTTCGATAGGTTTAAT
TATAATGATTAACTAGCCTTTAACCCGTGCAAAGCACGGGCGCTTATATA
ACTCGTAATTTATTAATTATAATTTAAATCTTAACCCCATTTTATTAGTA
TTTTAGTATTAATAATTTGGATTTTAGTTAAATTATATTAACCAACTGAT
TATATAAATTTAGCTTTTACAATTTATTATCTATCTATAATTATTTTTCT
GATATTAATATGTGATAGTTTATTATTCAATTAATTTTAAATCAAAATTT
TCATATTTCATATATCTTCATATGTTACGTAATGGTTCGTGTTTGTAATG
AAACAGAACACATTAGAGTTAAATTTCGAGTAGAATACGTTAAAATTAAA
AAGTTAGAGTTAAATTTCGAGTAGAATACGTTATAATTAAAAAGTATTGT
CTCTAATTATTTATATGTATTTTAGACAAAAGATATTCAAAATTGTATAT
TTATAATTAGTTATACATTTATCTATAAATTTTTTTTAATATTAATATTT
GTTATTAACAGCAGTTTCATTTTTTTCACCTTTAACCTCAAGCAAAGCAC
GGGCGTGTATATGATTCGTAATTTATTAATTATAATTTAATTCTTAACAC
CATTTTATTAGTATTTTAGTATTAATGAATTGGATTTTAGTTAAATTATA
TTAACCAACTCGTTATATCTCCTCACGGAAATTTAGCTTTCACAATTTAT
TATCTATCTATAAATATTTTTCTATATTAATATGTGACAGTTTATTATTC
AATTTAAATCAAAATTTTCATATTTTATATATCTTCATATGTTACATAAT
GGCTCGTGTTTGTAATGAAATAGAATACGTAAGTGTTAAATTTCGAGTAG
AATATGTTATAATTAAAAAGTAGCGTCTTTAATTATTTATATGTATTTTA
GACAAAAGATATTCAAAATTGTATATATATGATTAGTTATACGTTTATTT
ATAAGTAATTTTTAGTATTAATATATGTTATTAACAGTAGTTTCACCCTT
TTTTTTAACTAATTATAAATTATATTTAAAAAGATATTAATATACATAAG
TAGTGTTTTTAGTATATGAAACTGTTTAATAGATATCTATTTGTAGACTT
TTAGTTTTAGAGGAGAGTACCAAACCAAAATTTTGTACGACTACAAATTA
TACATATGTTGGCTTTTTATAGTATAGTATAGATGAAACTGTTTAATAGA
TATCTATTTGTAGACTTTTAGTTTTAGAGGAGAGTACCAAACCAAAATTT
TGTACGACTACAAATTATACATATGTTGGCTTTTTATAGTATAGTATAGA
TAGTATAGATTATATTTTAAAAATAATTATCACTATAATTTAAAAGTTTA
TTATTCACAAAGTATTTCAAAATTGTATTATTTGTTTAGAATAAATTGAG
TTAACGGAAAAAACATAATAAATGTCGGTGCGAAAAACAAAATAAAAAGA
GAAAACGAGTAGAAAGCCCGATAAAACAAGCCATTGTTCCATTTAGTACA
GTGGGATCCACCTGCATCTCTATCTATCTTCCTCCATCTCTTTTATTGCA
TCCATCTGTTTGTATAAACTTGCATATTCCCATCCTTTTCATCTTCATCT
TTACAAGTACATATATATATAGTACATACACACTTCAAGCTTTCTGGGTT
CTCTTTCTTGTCCTGATCAAGCTACTTCAAGAACCACTTTTTTTACTACA
GTAAAAGGTGCTTACTTTTCTATGTCCTGATCAAGTTTAATTTTTTTTTT
TTTTTTTTTTTTTTTTTGAGTTGAATTTGGTTTGTTATGGTTGATTACTT
CTGGGTTTGTGTTGTATGTGCTTGACAATGATGTTGGTTTTGGTTTTATT
AGGGTTTGATAGTTGATCATGGTGGTTGAATTATGGGTTTTGATAACTGA
TAAGTATGTTGTGTATGTACATGGTGTGTTATGAGTCATTGTAGTGTAGA
AACTGCTTAGTGATAAGTTGAAATAAGTAGTTTTATTAGTACTAATCACG
AGTCACATTACTGCAAATTTTCGGTTTGCGGTTTGTAGGCTGATGTAGTT
TGTATTTGAAGGAATTACTATTGATATGCACATTGATTGTTTGTTTCTTT
TCGTTTATCGGTATTGTATTGGTAGTGTATGATCAAAGGGTTGAGGTTTA
TGGATTCGGCCTTATAGATAGACTACAGGATGAGGTGAATTTATACTGAA
ATTGATAATAGAGATTGACAATTTGTCTACATTTTGTAGTATATGGTTAA
TATTACGAGTTGGGTTGGTTTGTGAGAAGTGGAGAAGATAATTGTGCATA
ACTGGACAATATTGATAAAGTGGTGAGCAGGGTTATTGATCGATCTAGTG
AGCTTCAGTACAACTTGGAATTTTTGATACTAGGATGGGTGCTAAACTTA
CCTGCTGTTGGGAGCCGGAACACAATGGAGGGTCAGACAACCATCATCTT
GGTACATAATGTGTCCCCCTTCCTAGTTTTTTCATTGGCGATGAGGATTT
GTTCACAACTTTTAAATATGTTTGACAGAACATGAGGACTCTGGTGAATC
TGGAGATTTGCCGTCTTTTCGTCAGTACACTGTAGAACAATTAAGGATTG
CTACATCTGGATTTGCGGTGGAGAACATAGTCTCTGAACATGGAGAGAAG
GCTCCAAATGTGGTTTACAAAGGGAAGTTAGAGAATCAAAGGCGGGTTGC
AATCAAACGCTTCAGTAGATCTGCATGGCCTGATCGTCAGCAGTTCCTTG
TAAGTGTCATGCTGATTTTTGTTTTACTTTTTATTTTGGGAAATTCTTGA
TGGTACACTCGTAGAATTGGCTTTTCTCAATGATACCATCGCATTTTTGA
CTTCCACCAGTGGTACCCTCGTACTTTTAATTTACTTTCTATGGTACACT
CGTGTACTTTTGATCTACACTCTATTGATTCCAGACTGTAGAACAAATTG
TATATTTGAAATCCTTGCGAAAAGTTACATAAAATAGACCCTTAAATCAT
GTAAAACAGAGTCAAAATGAGTGAGATATTAATGACAAAGTTTGGTTATG
AAGTTTGAATATATGTCACTCATCTTGACTCTGTTTTACATGATTAAAGG
GTCTATTTTATGTAATTTTTCGCAAAGATTTCAAATATACAATTTGTTTT
ACAGTTTGAAATCAATAAAGAGTAAATTAAAAGTACACGAGGGTACCATA
GAGAGTAAATTAAAAGTACGAGCGTACCATCGGCAGAAGTCAAAAATGCG
ATGGTACCATTGAGAAAAGCCAATTCTATGAGTGTACCATCAAGAATTTC
CCTTTTATTTTTCATTCTTGAATGGCCCTAAATGTAGTTTAAAGCTCAGT
TGCTTTGAAGATTCTTAAGTTTAGATAGTATTTGTCAATCTCAACTGTTG
TTTTCTTAATGTTATATTCCCTTACCTCCTAGCTCGTTGTTGCTGTTTTT
GTGATTACCCAGAGCTGAGCTAACAATATAACATTAATTAACGAATTGCC
TAATCACTTCAATTATGCAATACGGACTTGCCTGCAACTACTTACCATTG
GCTATACACTTTCCAATCTAGGTTATGAATGCTGCCTGTGTTTTTGGTCC
AATAATAGTTCACCTCTTTTTACGGAAACCATGTATCTTTTCTTCTTTAT
ATACATAACCTGATAATTAATAATGCAGGAAGAAGCGAGAGCTGTCGGGC
AACTCAGAAATCATAGATTGGCAAATCTACTTGGTTGTTGTTGTGAAGGT
GATGAGAGACTTCTGGTCGCAGAATTTATGCCTAATGAGACTCTTGCAAA
ACACCTTTTTCACTGTAAGTTTTTTTATAGAAAACATATATTGTTTCATC
ACCCATTTCATCTTTTAAGACGCAAATATACGTTAAGGTTGATTGAGACT
TGTGCAGAACAACTTTTTTGGTCTCTTTCTATGCTACCTTATTTTTCTGC
AAGTCTGTTAGCTATAACTTCTTTTGTCAAATCACCTATTTGTTTTGGTT
TCAAACACCCAAGATTACATTGCTTTTGATTGGTGTATTAGGGGATAAAG
AGAAAGTTAGTGTACCATTAGTTAGGGGAAAAAACCAAGTGTCTGCTTAC
AGCCTCTTTTCATCATCTAAAATTATTTTTACCTAAGATTCTATTTATTT
CAAGAACATATCTTTTTGTTTATTAAAGTCTGGTGTGTCGTGTCAGATAA
CATAGCACTATAGCAGATGCTGTGTACTGTCGAACTTCCTGTTCCTTGAA
TTAAATTATTTGTCTTTCAATGCAAGTTTGTAATTAGATCTCAGAAAATA
AGACCAGTGAAGGATATGTCTACATCTTACGTTACAGAAGTCTATTACAA
ATAATAAGTATCATCAGTAGATATTTTAACGGAATAAGGGAACAAGACTA
GACTTAAAAGAACCCTAGATAAATGAGGACAATTGTTTGAAATTACCAGT
AAGACGGTTAAATTAAGTCATTCTACTACAATTTGGGTTGTTATTCTTGG
TTTGGTTGGAAAGAATGGGGTGGAATAGATTGACACAAAAACATGTCACA
AGAAATACTAATGGAAGAGGGAAAATTTGAGTCTGCAAAATGTTCAACGA
TAAGGTTTGTAAAGTATGTGAGTAGGAAGTAGGAATAAGCATTGACTCTA
GATGGGATTAGTGGGGACTGGGATCATGTAATCGATTTTTATATAAGGTT
GAATCAGTGGAACGTATTGAGGAATTAAACTAATATTAAATGCCGAAACA
ACAATACGAATTACTTTTCATTAGGATCCCATTTCATTTCTTTCAACCAC
AGGTAGATCAAATATTTCTTATTGTAAAGAGAGTAAATTATACTGTATTG
GCTTGTTGAAGTAACAGTATACCCTTTAAAGTTTTTCCTTAGCCAGAATG
CATTAGCAAAAGACATTACGGGGCACAAAATTCCTGTAAATATATGGAGT
CTTCTTTATACGTGGATATCGTGTTTTTTTATTAACTTGTTAGCTCTTTA
AGCGAATGTACATAGTTCTATATATCTATATTTAGATGCTCGAAGGATTT
TTTATGAGTAATTTAACTCCTCACTCAGTATAGTTGATGCTGGTGCTGTG
AAGAGTTACTTGTTGTTCAAAGATAGGATACAAGGATACCGCAATAGCAT
AGCCTTATTCATATCTATAAGAATACCGCAATGGCATGGCCTTAAATTCA
TATCTATAAGAAATCCCTATAATTGTATGCTATTATTTCAAGCATACAAG
GATATCTAAAGAAAATTCATATTGAATTGGTAAATATAACTAAATAACGT
GGTATCACCAGATATTTTTTTTCCGTGTAAATAGATTGATGGGTGTCTGT
CCTGTACTTTACTGGGTTGCAATTGGCATCTGCCTGTTAGTCAAGGGTGC
CTGTCCCTCCTCTTTTCATGATTTGGATTTATCTTAATCAAGTCATTGTC
TTAAATCTTGGTAGATAAAGATGTTCTTTCATTGTTGCGTAGGGGAATCA
CAACCTATGAAATGGGCCATGCGGTTGAGGGTGGCCATGTATCTTGCAGA
AGCTCTTGAATATTGTACAAGTAAAGGCCGTGCCCTGTATCATGATTTAA
ATGCTTACAGGATAGTCTTTGACGAAGTAAGTGTATTGTTTCACTTTTGT
AGTCTTCTGGCTTTTCAAGTATTTCAACTGAAATAATCATGTACTATCTG
TGTCACACCATAGGATGCTAATCCTAAACTCTCGTGCTTTGGGCTGATGA
AAAATAGCAGAGATGGAAAAAGTTACAGCACTAACTTGGCATTTACACCT
CCGGAGTACCTAAGAACTGGTATCAATACTTTGTTGTTCAATTTTAGCTA
TGCATGAACGTCTCAATAATAAAAATGGTTCCAAATAGTATGCCTAAATA
GATACTTGCTTTCCTAAATAGATACTTGCTTTTTCCAAAATAAATGACAT
TGGTAGGCATTGTTGTAAATTTATTATTTATGTCCTTTTATTGATGTGTC
CCTGAGATAGCTTCCTTGTCTTCACTGTAGGAAGAGTAACTCCAGAAAGT
GTGATGTATAGCTTCGGCACCCTTTTGCTAGACCTTCTTAGTGGAAAACA
TATTCCTCCTAGCCATGTAAGTTCCCTCTTTTTCCTCATGTTAATATTTT
GTGAATATCCTAGTTTCTGTTTGCTACTCACATTAGCCGCAAGATGCGTC
TATTGTTGTGAATATAAAGCAGGTGAGTGAGTAATGAAATTGTTACTAGA
CGAGAGTTGATTAGTTCATGTGCTAACAACGAGAAGTTTTATAACCGACC
AATGTAACAGCTAGTTGTATTTTTTATTAACCGTTGTGTGCAGTTATTCC
TACTTTTAGGTATTCTATAATTCATTATCAGAGCATGTCGAATGCTAGTG
CTGAAATACATATACTTATTGCTATATTAATATAGCATTTTTTTCCTCTC
CTGATTTTTATAGCGTTAAAGAGCTTTTTGTTGTCTACCACCTCATGCTT
TCTTAAATATCTTATGTTCCACTACCATATATCGTATCTACAATATTCGT
GCTCCAAGACGACGTCTTTGAGGCATGAGTTGAAATTTACAGTGTCCTAA
ATTAAACGATAGCCTTCCATCAGTGGAAGAGTAGGTCTTATTGACTAAAG
CCTTGTGCATCTATATGTTAGGAATTTTGGCTTTAAGTTTTGTTTTTTTT
TTATTTTCCCATTTTCCTTTGTTGCTTTTCTCAAGCTTGTCATATTATGT
TTGTCAAATTGACTTGTGTATGTTTATCTGAAATGTATTGCACAGAAATT
TTCTGTAAGAGATTTAGCAAGCACTTCGGTCGACCTAATGCAGAATGCTC
ATTCTTATGTAATATGTACCAGGCCCTTGATCTGATAAGGGACAGGAATC
TTGAGATGCTGACAGATTCTTGTCTGGAAGGCCAATTTTCTAATGATGAT
GGAACTGAGTTGGTGCGTTTAGCTTCAAGATGTTTACAATATGAGCCCCG
GGAGCGTCCGAATCCAAAATCTCTAGTTACTGCTTTAATACCTCTGCAGA
AGGAGACTGAGGTGTGTTGTTCTTTATCTAAAGCCTCAAAGTTGCTACAG
CTTTCTGGCACTTCAAGTCTTCAAACACCAACATGCTTTATTATATTATT
GAATTGATCAGTTATACAGTTGGATTTTATTTTTTCTTTCTGAAATTTAA
CAACCAATATCTAGTAATTATTTGTTTGAAAACTCTTGGAACAATTTAAG
ATTTTATGTAATATAGAATACTGATCGATTACCAGAGATACAACAGTATC
TGCCAAAGCAGTAGCTGGGCCACTGACGTGATAATTATACTGTGTCGTAG
ATAACTTTTCCGATAGTATGCGTCCTACTTGAATGCGGTTTCAAATGTCA
TGATTGTAACATCTACTGTTTGGCATGTATTTTAAATCTTAATCAACTGG
ATTTGGTGTCTTATTTAATTTTTTTTAAAGCACCCTTGGCAAGTATTAGG
CATCATTTTTTGTCACCCATCTTCACTGGTGTTGATTTATCATACCAACA
TAATGAATGGATGATTTAAAAATATAGTATGTTTTGTAGGTTTCCTCTCA
TGAACTTATGGGCATACCAGATAGTACTGCTGCTTTCCCCCTTACCCCGC
TTGGTGATGCTTGCTTAAGAAAGGATTTGACAGCTATACATGAGATTTTG
GAAATGCTCGGGTACAAAGATGATGAGGGCACTGCAACTGAGGTTTAATT
ATTTTTTTTCCACATTATATGCTTTTAAAATATGTCAATTTTACTATTGC
AACATAGTATCTACATATTGTTGGTTTGCGGGTTATATGGCATTCTTTTA
CTTTTAAAACTAAATTGATACCATTAAATTCACAGGAGCCCTTTTTGATT
AGCAAAAGATGTATATTGCTAATAAGCTTTTTTCTTTAGTTTCTATTATA
CATATTATTATAAATTGTTGAGGAAGATCCATACATCAACGTTCTTTGAA
ATATTTGCATATGTTCTACTCTTCTTAATTACTGCTTTTGTTCAAACGCT
AAAGTTGGTCTATGAAAATTGAGAGGTATTAGTGCAACAAGCATGTTGGT
TCTTGCTTTTCACCATCAAATGCCAGTCATATGCTTTATCTCATACTCCT
GCTGACTGATTGTTTCTGCCATTCATATATTCAGTTACTACCATTTTAGT
ATACTTAATAAAGGAAATTTTTATTATCAGAACGTCATACATTTTCTTGA
GTTTTGCATCTTCATGTTTTGCTTCTGATTCATCAGTTTTGCATCTTCAT
GTTGTATTCTCCTTCTCTTTTCTTTTTTTCTAAAAAAGACGTTTCTTATA
AGTCCTACCGGTCTCCGGTCTATTATATATGTACTGTATGCAAGGCCGTA
ACTAAAGGTCTTCTTAATAGTATTCTTTTTCCTTGTTACACGCAGCTTTC
ATTCGAAATGTGGACCAACCAGATGCAGGAAACTTTAAATTCAAAGAAGA
AAGGTGACGTCGCTTTTCGGCATAAAGAGTTTAGTGCTGCAATTGACTAC
TACACACAGGTATGAGAAGTCCATAGATATTGAATCAAGGGTAAATCTAA
ATGTGAGGTACTTAGATGGCTAAATTCCGGACTCCCCAATCTGTACAAAT
TAGATGTTGCAGAACTATCTCGTGTCTTCCTTTTTTAGGAATTAAAAATC
ACTCCAGCTGTTCATCAACTTATACTTATTCTGGCTTATAAGAGATAAGG
GGAAAGTGCTGGGCGAGGCTGGTACATATACACTTCATGAACATAGTAAT
ACAATCTTAAGTAACACACAAGAATAGTGCTTGTGCATATCAAGGATGAC
TTTGTGAGGCACAATTGTATACAGGATCCTTTTTATGTTATGTATTAGAC
TCAAATCATGATATATTGCACTTGTTTGCAGTTCATTGATGTAGGGATTA
GGGTCTCCCCCACTGTCTTTGCTCGCCGTAGCTTGTGTTACCTGATGACT
GAAAGGCCTCTAGAAGCTCTTAATGATGCCATGCAAGCACAGGTGATATC
CCCTGTTTGGCATATTGCATCTTATTTACAAGCTACTGCTCTTTCCACTC
TGGGGAGGGAGAATGAGGGACAAGTAGCACTTAAAGAAGGTACTGTCCTC
GAGGGAAAAAGGGATGAAAATCCATGA