- Iorizzo M, Ellison S, Senalik D, Zeng P, Satapoomin P, Huang J, Bowman M, Iovene M, Sanseverino W, Cavagnaro P, Yildiz M, Macko-Podgórni A, Moranska E, Grzebelus E, Grzebelus D, Ashrafi H, Zheng Z, Cheng S, Spooner D, Van Deynze A, Simon P. A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.. Nature genetics. 2016 06; 48(6):657-66.
- Osuna-Cruz CM, Paytuvi-Gallart A, Di Donato A, Sundesha V, Andolfo G, Aiese Cigliano R, Sanseverino W, Ercolano MR. PRGdb 3.0: a comprehensive platform for prediction and analysis of plant disease resistance genes.. Nucleic acids research. 2018 01 04; 46(D1):D1197-D1201.
PRGdb |
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Relationships |
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The mRNA, DCAR_027547, is a part of gene, DCAR_027547. |
The genetic_marker, MK000003 Q1/850, is adjacent to gene, DCAR_027547. |
The genetic_marker, MK000004 Q1/800Flanking, is adjacent to gene, DCAR_027547. |
Name | Description |
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An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |
Transcript Name | Identifier | Type | Location |
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DCAR_027547 | DCAR_027547.mRNA | mRNA | DCARv2_Chr8:21895147..21909857 |
ATGGATGCAGCAGTGGACAAAATGGTGACGTTTGGGTTTGAGGAGAAAGA
TATCAAGGAGACTGTGAAGGAGCTGTTAACGGTTTGATGCTTACTTCTTG
TTTTTTTAAAGATTTTTGTTGATTTATTGTGTAAGAATGTGTACCCGTTT
GTGTATTTGAGTGTTTTGTAGTGAAAAACATTAGTATGTATGTGCTTTTT
TATTTGGGGAATGTTTGATAATGTCATTGTTTTGTTGAGATTCTTTCTTG
GTTTATTGTGAAATTTGATCCCTTGTGTGAGAGTATTTTGCAGTGTAAAA
CAGTGGTGTGTATGCGATTTTATGTTTGTTTTGCACTTGCCCTTGTGATC
AGGAGGGCTTTGATTTTGGAAAATTTTAGATATCAAAAGGGGCTGATGTT
GTACTTTTTGGTGGGAATTATTACTGATTTTAGGTCTATGGTGGTGGACC
TGAAGCTTGGCCTGTTATTGAAGATGGCTGTTATAGTATGCTTCTTGAAA
TTCTTTTAAGCAAGAATTTAGAGAAAGATGAAGGCGGGCCAAGTCAGACC
CGAAATGAAGAGGGAACAAATGGACTTGAAGAGACAATGGTAATATATAC
TGTCCTTAAAAAATGTGTGAAGTATACATTTTTGATTTTGCCAATAATTT
ATATATATACCGATGATTAAGGGGATAATATAATCTTTATTATCTTAGGG
GATCAGTCTTCATTAGTTTATGTCTGAGGTGTTAGATTGTTGGGATAATG
GATGTCTAGAGGATAAGTTCATCAATTATCAGTAACCAAGACATGCCAAC
AGTAATTTTACGGAGTTGAAATATTGTGTTTATGACCATTAGCAATATGG
TGTATGTTTGTGTGAAGTTTGTGGAATACACTGTTAATCAGCCCATTGTT
AGCAATTTGTTCTTTATAGTGCCAAAGTTCTGTTATACGTTGTTGTGCTA
GGTGATTCTGCCAATACTCCATACTCTAGCTTTAACTGTGTCCTCTTCTA
TGTATTTCTACTAGAAATGTAATGCCTAAGTGATGACTGGATATTGGGTT
TTGCATGTGTACTAGTATAACCAATCACTTATTTATATTTGTGATAGCTT
ATTGTTTTAACATTTATCTTGTCATCAAAAGATAGCCATGTAAGCTAGAA
TTTATGAGAACAGAGTACAATATCTCCCTTTCCATTCCTAGCGTTCTTTA
TACAGCCATGCAGTTAATATAGTCCTCGCCCTGCGTCTTCACATATAATG
TTGTACATGATGTAATCTGATCTTTTTCCTACTTATTACTCTGGTTCAAC
TGTACGAACTACTGAGGATTTAGATGATATATATAGGCCTGCTACTATAG
AAAAAGTAAAGCACAAATTATGTGCTAAGTGGATCTACTGTAAACTGTTT
ATGCTTGATTTTAAGCTTGAAGTAAAATTGAAACTAGAGAAGTCTAAGAT
CAAGTTGTACATGGTATGAACAAGTGTACACCGCCTTATATCCTGATGAT
TATGTCGTGACAGCACAAAACAACAACAAACACAGCCAATTTGAGTGTTG
AATTTTGATTTAACTATATATATTGGATCGAGATGAAGATGGAGACAATG
CAGCCAAGAAACCAGCTATAGGTTCCTTTATAAGTCAACAGTCCAATACT
GCCTACTGAAGTTTTACAAACTCTAGCTTTTGTTAATCCCAGAAAAGATT
TTGTTGCACAGGGCAATGAATTGTTTAGGAAATGATTTATTGCAAATTCA
ATTGTATAGAATCCAATACTAATTTTAATATTTACCTTTGACTTGAGTTG
AGTACACTGACTGATTGTCATTATCATTCCTATAACTCTGTTTGACACTC
TTCGTCATGAAATATTTGAACCTTGATCTTTTGCTAAGCATCAGACCTGG
GCATTGGTTTGGATTTTTAGAGGTCTTCAGGTTAGTACATCAATTCTCAG
CAACCAAGAAACCAGCAATAAAATTTAGTATGGTATGTGTTCATGTGTAG
TCTTTGGGATGTATTAATTACTCCCTCCGTCCCCTTTTACACGTCCATTT
TCAAAATAAGTTGTCCCTATTTACATGTCCATTTACAATTTTAAAACAAA
TTTAATGATAGTTTTTCAGATCTATCCTCATAATTTGTAGTATTTCCAAG
TCTTGACTTATTAAAAATTTGGTTGAATTCAAGTTTTCAAGACATTAATT
AGGGGTATTCCATCATTTTCAAGGCATTAGTTAGAGGTATTCAAGAAAAA
AGTTAACATAATCAATTCTTCCTTGGTATGTGTGTTTTTTCCAAAATGGA
CATGTAAAAGGGGACGGAGGAAGTATTGTTTATCAACCCGGTAATAGCCT
TTTCTTTCTAGTGCCATGGTTCTGTTTAGCATCTGTTATTATGAAATATT
TTGATCTTGATCTTTTGTTTAGTATTTGACCAACTCCTTTTACTTATCAC
TCGTTTTCATTGCTATGCTAGATGATTGTCTGTCCATATTCCATACTTTA
GCTTAAACTTGCATCGTCCTCTATGTAGTACTATTAGGAATGTCGTGTCT
GGGTGATGCCTAGGAGGGTCGTAGTTTTTCATGCCTACAAGTTTAATAAT
GACATTGTATAATTATTTCTTTTGTTAGCACTTGTGTTATTATTGACATG
ATATTCTAATCCACCCTTTTCCATGTATGATAGACTAGGAGTCTGGGGAT
AAAGCCCCTTTAACGTCTATTGCTGTTCATTACTCTGGTTAACCAACTAA
ATATTTACTGAGGAGTTACACGTTCTAAACTGTTAATGCTTGTTGAACCT
TTGAGTGTAGATGGTAGGGAGAAGTCTGATGTCCCACGAGCCTATGCATG
GTATTAACAAGTCCACATCAATTAATATATTAACTTCGTAATGCAGCTAA
TCTAAGTGTTAATTTGATCTCATCTATAGATTTGCTTGTATGATTTTAAT
GTGTACTTGTCAAAGTTGCTTCATCAACATTCACCCACAGTCTGGTATTT
GCTCGTGTCCTTTTTCATACAACTTTGACAAGTACAACTTTAGCTTATTG
GAGTCCTCCAACTTCAGCTTTCGTTCATTTCAGATTAGTTTTTTTTTCCT
TTTGTATAATTAGCGTTTGACAAATCCTTAGAAATTATGTACTTACGTGT
ATTTTTACTAGTTTCTTGAATATAATAGTTTACTTGTTTGTTTATTGAGA
TTGCCTTATCGCTTATTGTTTATGTTGACTCAACTATGAGTTTATTGATT
TTCCTTTATGTTTTCAGTATGGATGGTGGAGAGTGGTCCGAGATTACTAA
GATAAAGTTGTTATACAATGACTTATATATTTATATTTTCCGGCAACATC
ACAAAACAACCGAAGAACACAGTCAGTTTAGGTGTTTAGTTGATTCAACT
ATGAGTTGATCACTACTTTATTATGTAGTGCACACTTGAAAGTTACCACA
TAAAGTCCACAGCCCTCCAACTTTGCCTTCACTCTCGTATTTCACATTAA
ATTCAACGTATGCATCATATAGGAGTTATGACCACAGCTTATGTAAATCC
CGGGTTATATTTTCAGTCAAACAGAAAGTAAATTTGTAACATATAATTTA
ATACAAATCTCACTGTATACTGCACTGGGTTCTTACCAAGTTAAATTATT
AATTGTTACTTAACTAGAATTAAGATGAGGACCTATTTCAAAGCATTGTC
TGCTTCAAAAGGCCGTGAAAAGATTGCCAACTTGTGATATGGACTGAGAT
AGTTCTCAGTTACAGTGAAGTGTTCTGCAGTGGACCTCATACATTGTAAG
CATCAGTCGGTGTTGTGTCATTGATTTTCATGTCATCTTCATTAATTTTA
TCAGTCTGTGTTGCGTCATTGATTTTCATGTCATCTTCATTAATTTTATG
TCATCTGCAAAATTCTCCCTGTTAGCATGTTCATTATGTGCCTTTAAGCT
ACTACTACCGGGTGGATCTCAGTGATAAAATTTAAGGGGAGCACGTTTTT
TAAATGGCAAAAAGCATTATTTGTCATTTCTTATAATGATATCAGATATA
CATAACATCACAAGTAGAGTATCTAATATAATTAGCTGTCTTCTGTTTTG
AACCTTTGCCTCTGTTTTAATTTCTTACAAAATCCAAGCGGCACTTTTTT
CTTCAAGGGGATGGCAGCTCATCGTTGTTAGCTGATTGCTTCTCCCTGGA
AACACTTATATTTTTAGTTTGTATGATATATGATGAACTATAGCTACTAC
TGTATCGCCTGAATATATAAAATTTTGTCTCTAATCATGAAGACATATAT
GTCTTAGGTTATGATTTTTACACTTATTGCTGTGACAAATTTATTACGAT
AAATTAAATTGGCAAGTATCAACCTTTGTGCATATGATTGCTTTTTGTAG
GCCGCTGGACTGGGGAATACTACACCATTAGGTGATACGGATCCTGCAAA
TGATGACGCGGAACAGTATATGGATACTACACCCGTAGGTAGTACTGAGC
CTCGACATGATGCTGAACCGCCTTTTTCCGACATCACTGATAATGCATCG
GGAACTGAGAACACAGGTACACAATAAGATTCTCATTATCATCTCAATTA
TAAAAAATAGGTAAAGTTTATGTCTCACCTATTTGTAGGATACAGAGTTA
TTGACTTTATGGTAAAACAATTTGAGTACATAAATGACAGTATAGCTATA
CATATGTCTTGTGGTTTGAATACCAAAATACTTAAGTTGCAGAATGTAGC
GAGATATTGGTTTTTACATATCAAATAATTAGAATGATGTTCTTGGTGCA
GGTATGGAAGAGTTTCGTGGAGGGGTTAACCTCGAAAATACTGAGACCAA
CCTACCCCCAGCCATCCTCACTCCACAACCACCTGTCAACACTATTCCAA
AGAAACCACGCAGGCCTTGTTACGGGTGGATCAGTGAAGACGAAGAGGAA
AATGATGGCATTGTGCATCTCCCTGCCAGAGTACCAGGGAAAACTGTACG
AAAATCTAGATGGGATGTTGGACCAAATGATATATAAACCTCAGCTACAT
CCATTCAGGAATTTTTTTATGGTTTTTGTTTTCTATCATGTCTTGGAAGT
TGTTTCTACATTTTTAGTGAGAAAACCCATGAATGGCTAGTTCTTATGAT
TAATGTAGAAGTATAACTTGGAGCAAAGTTCTCAAATAACCGCATTTCAT
ATGGTAAACTTTCAGCATCATTTTGAAGATTTTCTTAATGTGTTTCTTCC
TATTTTTTCAAGAGAAGTTTTCTTTTCAAATTCAGTGGCAAGTTTGATTT
TATATCAGGATGGCTTTGTGGTATAAACCAGGGTATTGGTTATATCTGCG
GCCTTAGTTCTATCATTCTGTTAAATGCATAAAATTGTTCATAGCTAAAT
GCTTACATGAATCCAGGTTCCATGAATCCCGACTTCTAGCTGCTACCTTG
AAGTGGAAAAAAAAAACCGATCAACTAGCTCTGTCGGCCTTCAAAGCAAA
GATTTTTCATGATCCTCGACGATCTTTTAGCTCATGGAATAGATTTTAAC
ATTTCAGGAGATTGCCAAGGTCAGCAAGTTGAAACGAGTTCTTCTTTCTA
ACAAGAAACTTTCAGGGAACCTTCTATGTTGGATGAGATACATTTCGAAA
ATAATGAATTAAACTGAACTATTCCTCCTGGTTTTGGGAATTTCCAGTGC
TAGTCTTCTTAGATTTGTCTCAGAATGACCTTAGTGAAACTATACCGACA
ATCCACATATGACATTTCACCTTTTTCTGTTCCAGTTATATATTTCACAT
GCAGGGACGAAGCCACACGTAAAACTCGCGATCGGTGGGAGTCTGGGACC
AATTCAAAATAATATTAAAAATATGTTGATATAAATTAAATCTATGAAAT
AATTTACATAAAATTCAAATATCCTGCGTTCGTTTGAATCATGAAATCTC
GAAATTCATCTATTAAATTCTCAGTATTATAAAGAAAAAAGTAAATTAAA
TCTTATATGCAGTATATTTAACTGTTAGGAAGGGGGGCCAGACCTAAAAT
TCTATATTCTTTAACAGAAAAAATAATATATATAATAAAACATTAGATGA
ATTTAATGTTTTTGAAATCCGTTAAATTATATTTAATAATATAATGATAA
TAATTAGACATATAGATTAAAATAATCATACTTATATTATGTATTATTTT
TTTTTGACAGTTATATTATGTATTAACTAATTAGGTGTGTTACCACTTAT
TATCTTGACTCGAACTTTCATATTTTCTAATAATAATATTCATAATTAAC
TTTAGTTCCAAGTTATTAATTTCTTTTGTGAAACGAGAAATAACTCCTAA
TTAATTATAATATAGACCGACAATATCTCTCAACAATTCACTTTATATTC
GGAATCTATCCTAACACAAATATGATTAAAAGATAAAATTAACTAATTCA
AAACGAAAAAAATATATGTACTGAGTTTTAAATAATCCGGGCTATTTAAC
TAGAGGCGACAGAGGAGAACACTTCTATATTTTAAATTTAGTATAATTAA
AAGTTATGAGGCGACGGTAAAAGATGGAGCACAATAAAAAGGTAATATAC
ACGATTGCATTCCTTGACTCGATAAAAGGGCTCTGATATTATTTTTAAGA
CTCTTAAAAGTTAAGGATGAAATTATTTTGAAAGCATGGTGATTTAATTT
TGAAGGTTTTTAGATGATTTTATTTTGAAAGTTTTTAGGAGTTAATTATG
ATTTTATTTTAAAGCCCTTAGAAGATAATGGTGATTTTATTTTGAACTTC
CTTAGAAAGCAAGGAACAGGCCACCCCTTAGGAGTTAAAGTAATTTAGAT
AATTTTATATTGAAGGCCTTTAAGAAGTTAGTAAGGGTGATTTTATTTTG
AAAATCCTTTAAATTTTTAAAGTCTTACGAGCGACTATTTTGAAGACCCT
TAAGAATTAAGAGTGATTTTAATTTGGAGGCAAAAATGATTGCATTTTGA
AGGCCCTTAGAAAATAAGAGTGATATTATTTTGAAGACCTTTATTAATTA
AGTGATATTATTTTGAAGGCTCTCAGGATTTATTTTGAAGGTCGTCAAGA
GTTAAGGGCGATATTATTTTGAAGGCGCTTAGGAGTTAAGGAAAGCCCTG
AGGATTAATAATATTTTGTAGATTACAGGGATGGCTCTGTTACTTGATGT
GTCTCTATTGCTCACTAAGAAGATCGAAGGGATTGCTCAATTGGTTAAGG
AGGGAATTTCTATCCTCTTGGTCACGGGTTCGAATCCTAAAATGAGAAGA
GTTTGTGATTATGCCTCCTAGACCAAAACTTGTCGCTTAAGTGCAATTTA
CCTGATTCACGTGATTTGCAAGATACTGCGTCAGCTCGTAGGTTTATCCG
GTGCGCATCCGAAGGATAGTGGCTTGCAGGTTCCTATGCGTCAGCTCGTA
GGTTTATCCGGTGCGCATCCGAAGGATAGTGGCTTGCAGGTTCCTACGGT
AAGAAAAACAGATATATCTCTCCGTCTTATTATAAAGACAAGAGGGATGT
CTCTGTTACTTATTTTGAAGACAAAAAAATTTATCACTACATCTTGCAAT
TGTGAAAAATCTTACAAGGAGCATGTTTTTAAGAAATCAATATACCGTGA
CAATTTTCAATCGTCATGCTAATTATAATTTGAATTGCGCTTGTAAAATA
TTTTTCTTATATTGTCGCAGTAATACATATGTCAAAAAAGAAATTTCAAA
CTTGCAAACTAAACTTGTCAAGTAGGACAGCCTGAACAAACTTGTAATTC
CCACAATGCTACACTATCTTCTAATTTCTATTTATTAAGTCGGATTAATA
ATTAATTACTTGACCACAAGTAATTATCCTTTCTCTGAAACGAGAAAAGA
ATCAATTATTACATAAAATTTCTTTAAATAATTTCAGAAGTTATCACAAC
TCGAAAATTATATAAGATTAATAGGGATAAATATCAAGTTGGTGACTCGT
TTCGTCCAAATGTATCAATTGAGTGACTGATTTCCAAATTGACTCAAATT
AGACACTCATTAGAAAAATTTGTATCAAAAATATCACTCTATCGTTAGTC
GAAATTTTGAAAATATTATATATTGAGTTTCACACATTTTTTATGAATGG
TATTACATCCAAATGAAAGATTCCGAGTCCTACTATTTTTGCTAATATTT
TTAGATTTTTAAAATTTTATCAACTATTTATTTTTAATTTTTTGATTGTT
TTATTTGATTTAAATAAAAATAAATAGGAGATAAATTTTTAAAAATCTAA
AAATATTATTTAAAATACTAGAACTCGGAATCTTTCATTTAGATGTAATA
CCATTCATAAAAAAATGTGCGAAACTCAATATATAGAACTTTCAAAATTT
CGACTAACGATAGAGTGATATTTTTGATACAAATTTTTCTAATGAGTGGC
TCATTTGAGTCAATTTAGGAATCAGTCACTCAATTGATACATTTGGACGA
AACGAGTCACCAACTTGATATTTATCCCAAGATTAAAATAATTTAATTCG
GCATTTTACGTAACTTATCCTTTAAGCCTCTTGAGATAGGCCCAATAACA
ACATAACTGGGCCTGCGTGGGCTTTTTTCTATAAAAACATAATTATGCAG
GCATTTTGGTACATAAGATCTGATATCTGAGACAAATCGACACATCTGTC
AAATCCAGACCCTATTTTTTCATAAACCCTAAAATTCCTGAAATCGAATT
CGCCGTCAACACATTCTTTTCTCTGTTACTCTCCGTTCTGCTTAAAACTT
GAAAACACAACATAAATTTCTCTAAAAAAATTAATTTCTCAAGTAACAAC
ATTCGTCTTCAACAAATTTGAGATTTACGGTATGTCTTCGACTAATTCTT
CTGTACTTCCATATATTTTTTTCGCTTTCTTGTATATGTATGGTATTTTT
TTTAATGACATCTCATTAGTTGTTTAGTTTGGTAACAAAATTTATCAGAT
CTAGGATGTGTTTCGATTAATTTCTTCACTACTTCCGTATATTTTTCCTT
TTTCGGTAACAAAATATATGCATTTTCCCGATCTCTTTCTCCATGTAAAT
AGGGATGTTAGTTTTACCCGACCTGATAGATACCCGACCCGTTCCAACCC
GATTACATCTACCTAAACCTGATCTGGATATGGATCTTATTTTTAGACTC
GAAAAAGTTTGGATCTTGATTTGGATCTTAAATATTTTGAACCGAGACCT
GACCCAAAATCCAAAATCCGATCCAAACCCGATAAAACCCGATACAAAAT
AATTATATGTGTGTGTGCGCGAATGTGAGTGTGTTAAATATATAGTATAT
ATATTCAAACATTATGATTTATTAGTTATGTGTGTTAATATTTTATATTC
TTAATTTTTTTTAAAGTTATCATATATTTTTTATAGGTATATCATTTTTA
ATTTTTAATTTATTTTATTTTATTAAGTATTCGAATTAAACCCGAACCCG
AACGGAAACCCGAAAAAACTCGACAGATCGGATTTAGATCTTCATTTTAG
ATACCCGGACCTGAACCGGAGCCGACCCGAAATATAATGGATCGGGTCTG
GATTTTCTTGGTTTGAATTTCCTTTAAAATGTTCTAGATATATTTGATTT
AAGTAAAAAAATTTATGACCGTTTGCATAATCTTGGGCTTCAAATCATAG
TTGTATAGTTCAATACATTGGTCAATTATGTGAATCTTTGACAGGACTTA
AGTTTTATAATTGCCATTGAAGGTTAAGAGGAGCTCTTGACGGGGCTTAA
GTTAACTCGAGACTTATAACAGTTATTATATCATCATCAATTTACAAGCA
GTTGTCTATATTCTACTGCTCTATATAAATATTTATAGAACTTGGTTAAA
TGGCGCCAATGTACAGCCAAAGTGTTTATGTATGTTCTCACCCCATTTAT
AATAGCTTCGTTTTCTTATATACTATAGGGAGGAAAAGGTTGCACAGTGA
GAGAAAAATGGCATCAAGGGGAAGAGGAAGAGGAAGAAGAGGAAGACCCA
GGAAGGTTCGGGGTTCTGATCTTCTGCATGGGTTTTTGGTTTCTTGCATT
TTCATGATTTTAAAAAAAATATTAGTTGTTTTTTAGCCAAGGGAGACTCG
GATGGATGCAGCCTTGGACGCGATGAAGGAATATGGTTTCAAGGAGAAGG
ATATTAAGCAAGCAGTGAAGGAGCTGTTAGAGGTTTGATGTTTACCACTA
GTTGTTTTTTAAGAGTAAAACATTGTTATGTGTGTGCTTTTTTATTTTGG
GAAATTTGTGAAGGGGATTTTTTGATAATGTCATTGTTTTGTTGAGACTC
TTTCTTTGTTTATAGTGAAATTTGACCCCCCTGTGTATGAGTATTTGGCA
GTGTAAAACAGTGCTATGCATGTGTATTTGTGTTTGTTTTGCACTTACCC
TTTTGATCCAAAGGTGCTAATGTTACACTTTTTGGGGGGATTATCCTGAT
TTTAGGTATACGGTGGGACTCAAGCCTGGCCACATATCGAAGATCATGGA
TATAGCATGCTTCTTGAAGTTATTTTAAGCAACCAGGAAGGGGAAAATGG
AGAAGCTGGGCCAAGCCAGACGGGAGCAAATCAACCCGAAGAGACAATGG
TACTATATAATGTCCTAAAAAAAAATTCTGTGAAGTATAAATTTTTGATT
TTGCTGATAATTTATATTTGAATGCCTAAATAGATGATTTATAGAATGAT
TATTGTTTTATATAACCTTTATTATTCACGGGGATTAGTTCAATAGTTTA
TTTCTGAGGTGTTGCATTGTTTAGATAATGGATGTCTAGAGATTAAGTTT
ATCAATTATCAGTAACCAAGTCACGCGAACAGTAAATTTTACGGATTTGC
AAATATTGTGTTTATGTCTTTATGACCATTAGCAATATGGTATATGTTTA
TGTGTAGTTTGTGGAAGACAATGTTAATCAGCCCATTGTTAGCAATTCGT
GCTTTATAGTGCAAAAGTTCTGTTGTACATGATGGTCTTTTCCCTAATTT
CAGCAACTATAGGAAACCAACAACAAAATTTACAGTCAGAGTAGTATGGT
ATGCCGTTCGTGTGTAGTCTGGGATGTATAGCTAGCACTGCATAGCATGA
ATATATAGAAGTTTGTCTCTAATCATGAAGCTGTACATGTCTTAGGTTAT
GATTAAATACTTATTGCTGTGACAAAGTTCTGATGGCAAGTTGCCGAGTA
TCACCCTCTTTGCATATGATCGCTTTTTTTGTAGGCCTCGGAGCCTCTAA
ATGGTGACACGGAACAAAATTTGGATACTGCACCAATAGGTAGTATGGAG
CCTCCAAATAATGCCGAACTGCCTATTCCTGACATCATTGAAAATGCACC
AAATAATGACAATACAGGTACACTATAAGAATTATCATCTCGTTTAAGAA
AAATAAGTTGAGTTTATGCCTCAGCTAATATTTGTATGATACAATGTTAT
TGACTTTATGGTGAAACAATGTTATTACATAAAGGTCAGGATAGCAAAAT
TTTGAATTGTAGAATGTAATATGATATTGGTCACCACACACATTGAATGA
TGAGAATGTTGTTCTCCATGGTGAAGGTATGGAAGACTCCCGTGGACTTG
ATGTTACTCTTATGGCCCCTGTTATCAGCTCAGATGTCAACCTCGGTAAT
ACTAACACCGAGCTTTCCCTGGCCATCCTCCCTCCACAAGCACCGGGGGG
AACTATTCCAAAGAAAAGACGCAGGCCTTGCTACGGGTGGATTGATGATG
ATGATGAAGACTTGAATGACATTGTGCATTTAAGACCTTCTTAGGGTGGT
CATTGATCTTATTGGTTGTCTAGCATATTTTGGCAAACCTATGAATGGCC
GGTTCTTATGAATTCTGTAGAATTTTAACTTGGAGCAAATTTTCGAAGTG
GTTGCATTTTCCATCTTTTATACTTCCAGCAAAATTTAAAGAGTTTATGT
ATTTGATTCTATCTATTTTTCCTTTTTGGATCCTTTGTTGCATTTCTTTT
CAAATTTAGAGTTAAGTTCGTTTTTATATTATAATCTCTTTGTGATATAA
TAATGCAGGCAGTGGTTATATTTGTGTGTTAGCCTGTGTGCCACCAATTC
AACAAAAATGTTCCCAAATACCAGAAGATGATTCTAACCTATCACTTTGC
AATTCAAAACACTAGTTTGTATAGTGTTTATTCTATTTTCATTCTTTAAC
GCTGTATGGTCTAGCGTTTTGAAATCCTAATTGGCATTTCATGTAGCGTT
TTCCTGTTAAAATGCAATAGATCTAGCGTTTTCTATTGAAAATCAAAATG
TTAGCTTATCTAGCATTAATATATTGTATTTAGGATTTTTCTCTCAACTC
TGTTATTTTTGTTATTTTTGGTATTATTTGTGGTTTCTGTGACTCTGGAC
CTTTCTCCGGTTTACCTATATCATTTTGTTAAACAAAAAAAAATTACAAC
ATATTTATCTTTTTATGCATGTTAAACATATTTTGCCATGGTAACGGGAA
ACGAAACAGATTGACTAGCATTGCTGGCCTTCAAAGCAAAAATTATTCAT
GATCCTCAAGGAGCATTTAGCTCATGAAATATGATTCTCCGCTTCTGCAA
TCAGGTAGGCATTACTTGCGGCAAGCTGCCAAGCAGCACAAGAGAGTAAC
CAGCATAAACCTCACGTCCAAAGGTTTTGTCGGCTCTGTCTCGTGATGCA
GGAAACATGATTTTCCTGAGTGATAGAGAAGTAGACCGTCTTTTCAGGCT
TAAAGTTCTAAGCTTCGGACAAAATGCTTTGGAAGGGAATATACCAGATA
CCTTGGGACGAGTAAGATCCTGACTTCTGTTTCCAATATCTCATCACTGA
ATGTCTTCAATTTGGCCAATAATCAACTTCAAGTAAGTATACCATCAGAC
TTTGGATCGACCCATTGCAATTTGCAATCTGCAAAAGATTCAGCTATCTG
ATAACAGATGAAGTGGAACCATCCCTATATCACTTTCTAATGCTTCAAAA
CTCCAAGTTATGGACTTGCAGTTCAACAGTTTTGCCGGTTTCATACCTGA
GATCTTGGATGGTTACTCTATCTTCAGAAATTAGGTCTGACAAATAATAA
TCTAGGATTTGGCGAAAAGGATGGCTTGAGTTCCATTGAATCGTTAATCA
ATTGTAGCAGCTTAATGATACTGGAACTAGGGGCCAATAAAATGCTCTGG
AAGGGAATATACCAGATACCTTGGGAAGAATGCGATCCTGATTCCTGACT
TCTGTTTTCAATCGTTAGTACATCAAATACAAGGGCTAGCAGTAGGAATT
AACAGATACAGGGTCGGTGTGCATCCACATTAATCCGGAGCATCCACTGA
CACTTGTTATTAGTAAATCAGACACAGGTGTAAGGTCATACAGGAAACAA
AAAGGAAAAGGGAGAGAGTAAAACCTCAGAATTAAGATGAGTGCGGGAGT
TTACAAATTCTGGAAGTATATACTATATCTGTTTCTCATCTGCCCGACTC
AAATCATCCCAACTTCTGCTGCTACGGTAAAGGGAAACGAAACAGATCAA
CTAGCATTGTTGGCCTTCAAAGAAAAAATTATCCATGATCCTCAAGGAGC
TTTTAGCTCATGGAATATGTCTCTCAACTTCTGCAGCTGGGCAGGCATCA
CATGCAGCAAACAACACAAAAGAGTCACCAGCATAAACCTTGCATCTAAG
GCATTTGTGGGCTCTTTACCGCGTGATATAGGAAACATAATTTTCCTGAC
TGAAATTGTTCTTACCAACAACAGTCTTCAAGGCACAATTCCTCAAGAAG
TCGACCGTCTTTTCAGTCTTAAAGTTCTAAGCTTGGGACGAAACGCTTTG
GAAGGAAATATACCAGATACCTTGGGACGAGTTAATAGGTTAGTCATTCT
CGAACTTTTTTCCAATAACCTATCTGGTACGATCCCGAATTCTGTTTTCA
ATCTCTCATCACTGAATGTCTTCAATTTGGCCAACAATCAACTTCAAGGA
AGTATACCAACAGACTTTGGTCTGACCCATCACAATCTACAGAAGATTCA
GCTATTTGATAACAGATTAAGTGGAAACATCCCTATATCACTTTCTAATG
CTTCAAAACTTCAAGTTATTCACTTGCAGTTCAACAATTTTAGTGGCCCC
ATATCAGTAGATTTTGGAAGGTTACTGTATCTTCAAAATTTAAGTCTGGA
ATATAATAATTTCGGACTTGGGGAACAGGGTGACTTGAAATTTATTGACT
CGTTAGTAAATTGCAGGAGCTTAAAGATATTGAAACTAGGGGCCAATAAC
TTACAAGGATCATTGCCCCGTTCTATTGCTAATCTATCAATCGAGCTAAC
GATGATAAGCTTAGCAGATAACTGTATATGTGGAAGCATCCCTCCCGACA
TATCCAAATTTATCAATTTGATCTTTTTATCTATAGAGCATAACAATTTC
ACGGGCATTATTCCCCCTGAAATCACCCAACTTGGAAAGTTGCAAAGCAT
TCTTCTTTCTAACAACAGACTTACAGGGAACATTCTGGCCTCCATAGGGA
ACCTATCTATGTTGGATGAAGTTCATTTAGAATATAATGAATTGAACGGA
ATAAAGCAGGGTATTGGTTATATCTGCGGCCTTAGTTCTATCATTCTGTT
AAATGCATAA