Relationships |
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The mRNA, DCAR_029124, is a part of gene, DCAR_029124. |
The genetic_marker, MK008543 9_1956392, is located in gene, DCAR_029124. |
The genetic_marker, MK011456 9_1956482, is located in gene, DCAR_029124. |
Name | Description |
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An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |
Transcript Name | Identifier | Type | Location |
---|---|---|---|
DCAR_029124 | DCAR_029124.mRNA | mRNA | DCARv2_Chr9:1942201..1961468 |
ATGAAATCGAGTGAGTACAGCGTGCCTGACGGTTCCAAATCATCTGTGTA
TAAGTTCTGGGGTGATGAGGGACGTGATATCGGGAAAGTGAAATGTCAAA
ACCCCTACACATGGACCCCCGGTGGGTTTTCCATTGATAATGATTCACTT
AACTGCAGACTTATGATCCTTGCAGTGTGCACTTTTGTTTCTTTGATTGT
TGGCGTAGCAACTAGAGGATGGCCATCAGTGGTGCAGTATGGTCTTGAAA
TTGTTTTAAGTTTCTTGTTAGTGGTCTTTGGCACAGCCATTAAAAGACAT
GTACAATTCAGACATTTTGAAAAGGAACTCTTAATTCATGATCCAAAAAC
AGTCAAATTTCTTGACGTAAAAGAAATCTGGCCATCAACTACAAATGTTT
CGCAAGCTCTCAAAGTCACACGTCTTGAGTTGCTTGCAGGCTTAGCTTTT
GTTTCTTTGATTCGTTCCTTAGCAACCGGAGAATGGCTGTCAGGGGACCT
GGATGGTCATGGTCTTGAAATTGTTTTAGCTATCTTGTTAGTGGTCATGA
TAGAAAAGTTCTGGATGGTATGTTTCCACAGACTTATTACTTATAAACTC
GATTGCTACTTTTAACTCTGGTTCTGTTTTTAATGAGTGTGTGGTAATAA
TATGCATCTTAGTTAACTATGTGTGCTGTCATGAACTCATTTTGCTTACA
TATGTCCCACTATTCATTCAAGTGGGACCGTTAAAAAATAAAACTGTAAT
CAGAGGATATGGAATTGTTACACATATCATTGAGTTTAATTTTTTTTTTC
TTATTGCAAATCCTTGGTATTTATACATTGAATAATAGCTATTTAATTAG
GACTCCCTAAACATTGCCAATTAGAGAATATATAAGAATTGTTATAGCTG
AATTGATATCATTGATGATAATCTTTTCTTTCTCATTGCAAAACTTTTGT
ATAGATACATTGAAAAATAGCTATATACAACCTCTGGTTGCTTTCCGAGT
ATATTCATTCCTTCTTTCCTATGTTGCAGGGCTCTTTTCTGTCTACTCTC
GTAGCTATTAATAAGCTACTTATTGCTTGTTACCCCATCTCTTTTTGTGC
CATTGTTACCAATGCACAACTTTCCAATTCTGCTTTAGAAGGTAAATCTT
CTGTTCTCATGAATCTCTGCAGAAATACTGAAAATTTTAACATTCAAAGA
TTACTGATACTGTTTTTTGCTATAGATACAGAACTCAATACTCTCTTTTC
TGAAACCATTTTTTTAAATTGTGCGACTCAAACAGGGGACTACACTTTAC
AGAACTTCAGTCGTAACGATGACGAACACCACCCTCTTATGTTGAGCGTT
AAGAAGAATTGGGTTCAGGTAGATTTCCTACTGCTATGAACTTCATTTAA
TCATTCTTTACCTTTAACTTTAGTTCTAATTTTAGCTTTTGTTTGCCCAG
GGCACATCAATACTTGACTGGACTGATATAATCATGTTCATTGGTATAAT
GTTGTTGGCTGTGACTCTATACTACCACAACTGGGTGCTCTCTGCCTTTT
GCATTCTTTGGACACATATCATAGATTTGATGCAGGCGCTAAAAATAAAG
ATGAAGCAAGGATTGGTTAAAGTATGTTTCTAATTAAGTTGAACTTAATC
TGCATCTGTGATTATGTTTTTTGTTGTGATTGCCATGGTTTTGTGACATA
AAGCGTGATGGTGCATCATGTGCATCATGACCAGTTGTGTTTGTTGTCAT
TAACTGATTTTTCTAGCACTTAAGAAAGGAGTGTGCAATCAAGTTCTAAT
CAGACAATTTGCTGAATAGATACGATTGATATTTTTGTTGTGCTATAGTC
CTTTCTTTCTTTGTGCAAGTGTTTGTAAATTTACTTGGAATCGTAGCTGT
AATTTCACTAACTGATACCTGCACGGTCCTCCCTTCCCCCTATTTGTTCT
CCCTTCACCCTTCTTTGAGTTATGACCATTCTATCATTATTTTCTACTGG
GACTATTTTGCCCTTGGCTCTGCGTCTTGATGAGGGTATATTACGTCAGG
ATAATTGCAGCCTGTTGGATCTGTGTAGGGTTGTCAATGGATCGGAAATC
CGATCGGATAGCTAATTGACCAGATATGGTCCTATGGATCATCTAAAAAA
AAAATTCGATCCGGTAATAATCCAATCTGATAACTACCGGATATAGATTT
AGGTTGATGCGATCTGATTATACTATAAACATAAATATATTATTTTTAAT
ATATGATATGTTATAAATATGCAATTAGTATTTTGGACTCTCACCTTTAT
GCAATACAACATTATGTTTACCAATATATCTCTGAGTTTTTATTCTATAA
TATACAATATCAAATAAGTTTAAAAATGTTAAGTTTTAATTGGTTATTTA
TATAATTTTCATTTTCACTAATAGATTGTATAAATTATTGACATTTGTAT
TGTGAAGTTATTTATTAGTGTGTTTGCATAAAATATTAATTGGTTAGAGA
TGTTCGGAGACAAAAATACAGTTCCTCTCAAATCACGGTACTTGCAGTTC
CAGTTATTATGGTTAGTGCTTCTCATATTATATGTGGTGGATTAATAACA
ATAATACAATTGTGGGTATGTAAGTCATGAGCTCAATATACGAAGAATGG
ATTTCATAATATCTGACTGGTTAGTTTGCTTTCTTGAAAACTAGTTTGTG
TTCAGTAAATTGAATGTTGTCTTTCTGTCTATGATGAATATGTTAGCATA
TAACATCACTATTTCTATACAATACTGTGTAACATTGAACTTCTGCCGGA
AATATCTAGTACAAGGTAAAATATTTATAGAAGTTGTGTATGCATGTTCT
TACTACTGTAATTTAAAATTGTTTATTTGGTTTAGCTATACTGTTCTGTC
AACATGGCAATCTGAAGTTTGTAATATAAAAAGTTATTGTTTGAGCTTGC
AAGATAATTGATAATACGTGGATAGCTTGACTTGTTTTCTTGGAAATTAG
ATGTGTTTCATGAATCAAAAATATATGTTTCTATCTATGATGAGTATGTT
GCATACTTGCATATAATATCAATTCCTGTACCTATTTGTTTAACAACAAG
CATTTGGTGAATATGTTTAGCGCAAGTGATAACTTAGAGAAATTGTGTAT
GCATCTTGTTACTTTTGTATTTTGAAGAAGTTTGTTTGGTTTGATTATAC
TATTCTGTCAGTGTCAAAGAAACTTTGTAATATTAAAACTTATTGTTTGA
GCCTGAAAGATAATTAGTACAAAAAAGAAAAAAAAAAGATTAGAACTTCA
ATCCTAGTATGTTGTCCTCATGAATGAGTAGCAAGAAAAGATGAAAGCCT
CGCGTGCCTTGTCCTTTGCCCCACAACTGCCTTCGCTTCCTTATGTAGAA
GTTTGCTCATCATTTTCCTCCCTTATATTGACAGCACATGAGTTCCCTAA
AACGCTCTTGGTTAGCTTTCACGTGTACAATCCACTTATTCCACATTTGG
TTTGCTTTGTCCTCATGTAATTGTTTTTAAAACCAGTCCTGTCCATAAAA
ATCAATTACTGTTTTTCAGTAGAAGTTAATGTTTATGATATATTTACAAC
AAACCCTTGGCAAAATTAAGAAAAAGTTTGTAAAGCTTATGCCAGAAATA
GTTTGTATTTGATCTGGCCTATATATGTATGTCATGGCAACTAGACCCCA
AACAAGCTTTACGAGACCTAGTTGTAGCACAGCTGCATAAGCAGCAGAAC
CAGATCCCTCCATTAAGTGAACCACGACAAGCCAACCCAAACGACCAACC
ACCAAGGAGTAAAGCAGTGAGTAAGTGAGTAAACCCTCCCTCCAGTCATG
CACCAATATTTATTGTTGAACAGGAACCAATTCCCTAAATCTCTTGTTAG
AAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGATTTAAAGTAAAGAG
TGTTTGGATAATGGCTACCAAACTTTAGAAGGTTGAGATATTGACTTGAG
TCCAGTGAACTTAAATACATCAATGTACTAACTGTTTTTTCTCAATTCAC
GATTTTCACAGCAATTTTATTTAGTTTTGTATGCACATATTTTGGTGACA
TTATTAAACATCATTTACAAGTAGTTTAACCTTTCCTCGAAAATATGATA
AAAGATTGTTGTCTGGATCTGTGTATACAATATGTCATGATATGATTTTA
ATATGATTGTTTATTTATCATTTTCAGCTTAAGGAAAACAACGTTCAGTA
TCTGCGACGTGGATATAAGCTATCTAACTTAGTTATAGCATCCCCGTTTT
TAGGTGGTGGACACAAATGGCAAGTTTGCATGGTAGTGGGCATTGCATTA
TTTTCCGTCATCTTTTATTGTGCCGAGGTATCTGTGGAACTGAAGAAATC
TCAAGTATCTATATCAAATTTCTTTTTGGAGTTGAGGAACAATAAGAAAG
TGTTGTTTTTCACTGCAAGGCATGTTTTGGAAATTTTTGTTCCTTTGTTA
TTTTCTCTACTAGCATATGTTCGTTTGATGGAGTTTGATAGAAAAAATCA
TTACACCGAAACTGATTGGCCAAGTTTTGCACTTGTTATACAAGTAGCCA
TTTTCGTGGTAGGATGGTTGCATGTGATCATGGATGATTGTAGTCCTGAC
TATTTTAGATAGGGGTGTGCACGGGTCGGTTCGGCTCGGTTTTTAGCTAA
AACCGTTACCGAACCGTACATGTCGGTTCGGTTCGGTTTCTACCTTAAAA
CCGTTACCGAACCGTTTCAAACGGTTTTTTCGGTTCGGTTAACCGTCGGT
TTGGTCCTGACTATTTTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
TTAGGGGTGTGCACGGGTCGGTTCGGCTCGGTTTTTAGCTAAAACCGTTA
CCGAACCGTACATGTCGGTTCGGTTCGGTTTCTACCTTAAAACCGTTACC
GAACCGTTTCAAACGGTTTTTTCGGTTCGGTTAACCGTCGGTTTGGTTAC
GGTTTTTTTCGGTTTCTTATAATAAAAAATATTATACAAATTTCATAATC
AACTCAAAAAAATTTTTAATAAAAAATAAGGTATAATCACTAATTAAATA
TTATTCATTAGTACGTATTTTACCAAAATAAATAAGAAAGAAAATATTTA
AAATATAAATACTTATTAATATAATCGAAATGAAGTAGATGAGCACTATT
ACGGTATGTAAAGATGATCCAGAAATGTAAAGTATGAATCAGCCCAGTGT
TTTTTTTTTTTTAAAGGAAACGAACTTGCATTACTTAGAAATTATCAGAA
TTCAGTACAAGACTTAGTTACACAGTATCTCTTTATGAAAATTATGTTAA
TTACTAACTACTCTACTACTCTCTCTACTTAAATATAACAAACTACTGTA
ACTTGCTGACTTGTAACTATAGTTGAGGTGGATAGTTCCTTGCTTAGCAG
ATAATATACCAATGTAATAATTTATTTTATATAAAATATATAATATATAA
TAAATTATATAATATTATATAAGCGGTTCGGTTTTTCGGTTCGGTTTTAG
GTGTAAAACCGTAACCGTCGGTTCGGTTAACCGTCGGTTCGGTTGAGCTA
CGGTTTTTTTCGGTTTTTTTCGGTTTCGGTTTTTTTCGGTGTGGTTTTTC
GGTTTTTCGGATCGGTTTCGGTTTTTCGGTTTTTTTTGCTCACCCCTAAT
TTTAGACACAATGACCAGAACCAGTTAATTCCAATGCAACCAGTAGACAC
CATTCCGCACAAAGGGAATGAAATAATTTTGAAAGACAGTCTACCTGAAG
AATTTTCTGAAGAGGGTGGAGATGTTAATGACTTGACAGTGGCGCAGGGC
TCTGCCGCAAATGGTGCCAGCGTATCACAGATGGTACTTGAGGTTGCTGA
GGAACTTTGTTAAGTGGCTGCAGATGTACATTTATAGGTTTAAGTAAGCG
GCGTAGGTCAAAATATATGTAAATGTTGGCTTTGTTAGTAGGCCGAGTCC
ATTATACTATGTACACAGGTGTAAAAATGTAGAAGTTTCTTGGCGCAGGG
CAACATATACATTTTTGTTAGTAGGCCGAGTCCAGAACACTTGTGTAATA
TGTTGTCTCATCAATAGAGAAACTTGTTACCCTAACAATTATCGTGTGTT
CCCTGGTTTGGTGGATGGCTCCAGCCTGCAACTACCATAACCATCTAACA
TCTATTGCTTTATTGGAACTTTCTGCAAGTTGCAACTATTGCTTCCACCC
CCTTCACTTGGGAGGGATAGATGGTTAAACATTGGTAGAAGTTGTGTGGA
TAGTTTATGCCAACCTGCAGGAACTTAGAACTGGATCAAGTCTCAAGGGT
ACTAGAAGTCTAGAACTATATGAATTTTCCTGTTTCTTTACCTGGTTTTC
ATTGTAAACACCGGATCCCTTAAATTTACAAATTTTAACTCAAAACCGTT
GCTAAGCGCAGAATTTTATATGTTTAGGAGATTTTGATTGGTGTGGTGGT
TGTATATGCAGTGGATCAGCCATTATTAAGATATGAGAGTCAATAAAAAT
GAGTCAAACTTAAAAAATTCCGCGCTTAGCATGTGCTTATGGACACACAC
TAATAAGTTTCAAACTTTACTATTTAAGCTCCAGTTCAATTTTTTCATTT
ATCCAAATGACATTATTATCACTAATACGATTGAAACATGTGGAAAAGGC
ACGAGTAAATATGGTATAAATTTTGAAATTGGAGTTTAAATAGTAAAATG
TGTAGCTTATTTACCAATTTTCTAAAAAATCAGAAATATACCTTAAAAAA
GGTTATATACATTTTTTTAATATAATGTATTAGATAGGTAAATGATATTA
TATAATTTTTCATTCGAATGTAACCGAAAATAAAATAAACTATTAATTTG
AATCCGAGTGAAAAATGAAAAATATATCTCTCCCCCGAGGTGGTAAGAAA
AGGGGTCTCTAAATTGTGAATCCGTGTGATAAGCGTGTGTGATAAACGGT
TCAGATCTTCATCCCAGAACTTTCAACAATGTTTCATACACCACGCGTTC
ATTTCACTCGCTCGTTTTTTTAAGTCTATTGAAAGTTCGAAGACTTTTTA
TACCTGGTCTTCGCGTTCTCAAATTATTTCTATTTCTATTTCTATTTCTA
TTCTCAACGTTTTTTAGTTTCGTTATCGCACGGACCACCAGTGCATAAAT
GCTGTGACACGAGCATGGGAACCGAAGCATATTTCAGCGGTGCCAGCAAA
GTGTTGACGAGGTTTTGTTAAGGTATGAGGCTCACAACTATGAATCTTAA
CTTTAATTCTTATTTTAACTGTTTTTTGGTCTGGTCGAGTTATTGTTGAA
CCTCGATTAAGTAATGTAACTGCGTAGTATTTTCACTCTCGATAAAGTAA
TATTTTCTTCGGATACCGGTATTTATATGAATCTGAGTTTTGATTATTGA
TCCAACTATTTTTTTAATCTTGCAGAACTACTGTTCTCTTGTTATATACT
CATATTGGCTAAAATTTTAGCATTCTCATAGAAGAAATTTTTTTTGCTGA
TTTTTCTGGTACATTCCTCTGCACAACCTTCAGCGGCGACTAAAGACGGT
AACCAACTTGTATTCTTATCATTTCTGTTATTCTCAATCTGTACAGAACT
ACGCAATTTTTTTGATTACTTGAAGATTAGTATAACTGTTCTGCTGTAAA
TAAAGAGCCTGATTCTTTTTTTTCTCTATGAATATTGAAAATTTTGCTTC
GTGTAGTTCCAACGGAGATGTACAACTTAACTTGTTACTACCCTCACAAA
CGCCTTCCTCATAGGTGGAGGATTGAGAGCTGGATGAAAGTACGCTTCTT
ACACTGACATTTTATTATTAACTTTTAGCTTTGAGTCTAATAGCGGTTAT
TTGGCCAGGGCACAACGAGTGTTGACTTGCCAAATATGGTGCTGTTTACT
AGTTTAATGTTTACGGTGTTGTGCTTCTACAACAAGTGTGGATCTCTCTT
TACCGGTTTCTTGATTAAGGAGTTCAACAACTCGACTGAGAAGATCAAAA
ACCTGGTTGAAAAGATAGGGATTAAGGTGTGTTTTAATTTTGCTATGAGT
ATCTGTGACTTCACATTTTACTGTACCTGCCTTGGTTTGCAAATTTGGCG
ATCAATAATGAATTGTGATTGTGTTTGGTTGCTTCTCATTCTAGACTTCG
CTAATGGTGCTTACCAAAGAACATGTACGAATATATATAATCGCTATGGA
AACCTTTTCTGCTTGATTCTCATCAATTCCAACTGTTATGATTATAAACT
TCTGCAGTTGTGTCTTTCATTATGCTAGATGAGGGGTTAATAGGCTAAAA
CATAATCGAACTGACCTAAAACTTGCTTGGGTTACTCAATTAAAATTAAT
AAGTTAGTTTTTAATATAAAAATTACAATTGAAATCAACTTTGTACTTAT
GAATCATGTAAAAGATAATTTAGTCTTTCCTATGTGATGTAGGTGGATCG
ATTAAACTGCTTATGATACACTGTGGGTTATGGGGAGTCATTAGCTTAAA
GTAGAAAGAATAGAGTCGAGTAGTCGACATAGTATGACCTTAACCTCTTG
TAGGAAAGATTGGTGGTACAATTGCCACGCCAAAACCCACAATCTGCAAC
TTGAGATATTGGTCTTCAGTTTAGAAAGTTCATATCCATTAATGTAGCCA
AGTGATCACTTGCAATACAATTTTATCATAGTACATAACAATTTAATGTA
CTTCAATATGCAAAAAAATAAAAATATTTCATCGAGATTGGTTCTCTGTA
TAAAAATACAATATGTTTTGATATTATTGTTTACTTTTTTTCAGCTCAAG
GAGAACAGGATCGAATATTTGGGAGCTGTATTTCAGATATTAAACATAGC
TTTTTCATCCTCTCTTTTAAGGGATGCACAGAAATGGAAATCGAACTTCA
TAGCAGGCATGGCGATATTTGCTTTTCTCTGTTACTGTGTTGAGGTCTCT
GTGGAACTGAGGAGACTTGAAGTTACCAGAACATATATTCGTCCCGAGAG
GATGAACAATATAATAGTCTGGTTTTTCACTAAAAAGCATGTTTTGCAGT
GCCTTGTTCCTCTATTAACATGTGTTTATAAAAGCCATCACATGGAAGAT
ACTCGGGAAAGTGTTTCATTTATAATTCAAATGTCCATTCATGTTGTAGG
TCAGCTGGCATTCATGTATGCCCGTGATCGATACTATTTCTATGCAGCCA
ATTGACAACATTTCGCACGAAGGGGATGAAATATTTGAGAAAGACAAACA
TGTCGTTGCCGAGAAGTTGCAGATGCAAATGTAGTGAAGGGTCACAGAGC
CACGAGACATGGTGAAGAAATCTGCTCTGGCATTGACAGTAATGCCTGAG
TTAGCCTGTTGGCAATTTACTAGTGTTACATAAGATTCAAGTTGTTTGGG
GACAATGCCATCAATTGAAATTGTTGAGATCTTTTACAATTGATTTTTCT
GTTTTGTCTTGAAAAATTAGTCTTACATGTCTTACAAATTGGGATGAGCG
AGATTCGAATCGAGGACTTGAGAAGACAACAGTTTTTATGTGTTTAATTA
CATTAGAATGTTAATTCTATATACTGCTTGGAACTCGTTATTTTGAATTC
TATCAATGCGTTATATGTGATTGCATCTTAAATTTCACATCTCGAAAGTC
AATCAAAATATCCATTAGCAAAATTGTCTGTTAAGTTAAACTAGTGCAGG
TTAATTACCTTGTATTAAAATTTTCTACTGCTTGCTGCTCAAACAGATGT
CAATGAAATACATGCAACCCTGTCATAAAGATCTGAACAAGCAGGAGAGC
AATAGAATCATGTTCAAAGAATACAAATACAAAACAATTTTATTACCAGA
AGCAATGAATAACTTACAAGTTACAAGGGGCTGGAAAGAGCTTGACTGAC
AACTAACTAATGCCAAATTGCCAATGCCAAGGCCTGACACTACAGTAAAA
GTTGTCAATTGGCTGCGTAGAAATAGCATCGATCACGGGCATACATGAAC
GCCAGCCGACATTTGAATCATAATCACGGGCATACATGAATGCCAGCTGA
CATTTGAATCATATTCACGACACAATAAAAGTGCACGACACGTTCATATA
TTTAAAAGTGATCTCATTATATTTAAATAGTATCGATCACTGGCGCACAT
GAAAGTACACGACACGATCACAAGAAAGTGTTTCATTTATAATTCAAATG
GCCATATATGTTTAATCATGCCCGACCTATTTAAAAGTATCTACTGTATT
TTTTAGCTAGTGTACTTGAAGATGATAATCATACCCGATCTATTTAAAAT
TCATGTATTTTATGTCAATCTCATTTCCGTTTCGAATGTGTCATGTACAA
TGAATATAAATATAATAATAAAATTTAATCAAAATATATTTTAAAATACA
TTTTCATATGTTATTTTATGAAATTTATATATTAAATCTTTATATTTGTA
TACAATTTATAATTTTTACATGAAAAATATACTTATTTATTTAAATATAT
ATATTTCTATGATGTACATTCGTATATGTTTGTATATGTAAAATGAAAAA
TCATATCCAACACTAAATACCTTCGTGTTAATGTATCAAAAAATGAAATC
TAAACACTAATTTTCGATATCTTGTCGTCGGTGTAGTAATGTTTTTTTTG
TTGTGTACCGAATCACTAGGTTGGATGTAAAAGTGCTAAATTGTGTTCCG
TCTTCCGTGTGATACGCGTGTGTGATAAACGGTTAAGATCGTTATTCCAG
AACGTTCCAAACAGTGGCGCGACCAAAACTCACTTTCATTTGACTCGCTC
ATTATTTTAAGTCAGCAGTCTATTACTACCTCGTCTTTGCTTTCTTAAAT
CTCAACGCTGTCTAGTCTCCACCAACAGATGATAAATGCTCTGACACGAG
CACGGCGACCCAAGTTTATTTCAGCTCTCTCAGTAAAGTGTTGACGAGGT
TTCGATATGGTAAGCGTCCGACAACATTCAATCTTAGCTTTCTTTCTTAT
TCGAACTGTTTTTATGTCAGCTCGAGTTTACAGTTAAACCTCGGTTAAGT
AATACGAGGTGGTGTATTTTTACTCTCGAAAAAGTAATATTTTTCTTGAT
AGTAATGTTGTTATTTTAAAGAGATTTAATTTGTGAAAAACAATTGCGAC
GCGTTGTCTAGTTTTGTCAACTGTTTTTCTGCTTCAATTCTATCTTGCTG
CCGTTTACTCGTTTCGCTTGCACAAAGTGTAAAAATTGGTTAAAATCTGG
GCGTTCTCATAACAGAATTTTTTGAATATTGCAGAACTACTGTTCTCTTG
TTATTTCTTGATTGAAAGGAGTTTTTTTTCGGTACCATCTTGTTGCTGAT
TTTTTCGGTACTTTCCTCTGCACAACCGTCAGATTCGACTAAAGAAGGTA
GCTCTTGTATTCTTATCATTTCTGTAGTGTTGGAAAAAAGGGAATCAGAC
TTAATCAGTGAAGTCACAGAACAAGTATTAATCAAAGATTAATTGGATTG
ATCGGGAATTAATCGGATGAACAACTTAACTTATTGAAAATTTTGTTTCG
CCTAGTTTCCATGAAGATGTACAACTTAACTTGATACTCTCCTCACAAAC
GCCTTCCTCGTGGGTGGAGGATTAAGAGCTGGATGGAAGTACGCTTCTCA
CTACTGACACTCTATTATTTACCTTTTAATTTTTGACTCTAATAGCTGTT
ATGTGGCCAGGGCGCAACAATCGCTGACTTGCCAGAGATAGTCCTGTTTA
CTGGTTTAATGTTTGCGATTTTGTGCTTCTACAACAAATGTGGATCTCTC
TTTACCAGTTTCTTGGTTGAGGAGTTTAACAACTTGACTGAGAAGTTTAA
AACCCTGGTTGAAAAGATAGGGATTGAGGTGTGTTTTAATTTTGCTGTGA
GTATCTGTGACTTCACATTTTACTGTAACCGCCTTGGTTTGAAATTTGGT
GCTCAATAATGAATTGTGATTGTGTTTAGTTGCTTCTTGTAAGACTTCGC
TAATGGTGTTAATCAAAGAACATGTAGGAATATATATAATCGCTATTGAT
TCTCTTCAATTCCAACAGTTATGATTATAAGCTTCTTCAGTTGTGTCTTT
CATATATGCTAGATGCGTGTTTTTTTGGCTAAATGCTCATTAATCCTTAT
AAAAAGTGAAAATTTCCCCCAAACAGCAATAATAAATTTGTTTTGTGTCT
GTATACTACAATCTTTTAACCTCTTGTTAGAAATGTCGGTGATGTAATTG
CCGAGCCAAAACCCACAATCTGCAAGTTGAGATATTGGTCTTGAGTTTAT
AAAAATATAGTGTAGTTTAATATGCAAAAAAAGTGTTGAAATCTAACCTT
TCCTTTTCAAGCTGGTTCTCCTATCTCAAGGAATGTGATATGTGTGTCTT
AAATACAATATGTTTTAATTTCATCGTTTACTTTTCACAGCTCAAGGAGA
ACAAGATCGAGTATTTGGGAGCTGTATTTCAGATATTAAACATAGCTTTT
TCATCCTCTCTTTTAAGGGATGCACAGATATGGAAATCTTACTTCATAGC
AGGCATAGGAATATTTACTTTTCTCTGTTACTGTGTTGAGGTCTCTGTGG
AACTGAGGAGACTTGAGGTTATTAGAACATATATCCGTCCCGAGAGGATG
AACAATATAGTAGTTTGGTTTTTTACTAAACAGCATGTTTTGCAGTGCCT
CATCCCTCTAGCAACATGTGTTTATAAAAGCCATCACATGGAAGATGCTC
AAGAAAGTGTTTCATTTATAATTCAAATGTCCATTCATGTTGTAGCTCAG
CTGGCATTCATGTATGCCCGTGATCGATACTATTTCTATGCAGCCAATTG
ACAACATTTCGCACAAAGGGAAAGAAACATTTGAGGAAGACAAACACGTC
GTTGCTGAGAAGTTGCAGATGCAATTGTAGTGAAGGGGCACAGAGACATG
GAGAAGAAATCTGCTGTGGCACTGACAGTGATGCCTGAGCTAGCCTGTTG
ACACGAGTACATGTAATTTACCAGTGTTACATAAGATTTGAGTTGTTTCT
GGAACATGACATCAATCAGAAACTTAAAGTTTGCTTTAGCTTTTTAGCAA
TAACTCCAAATTCATTCGTAAAATATATTAGGTTTAAATTTTGTGCTGAG
TTCTGACAATATCAATCTGAAATTGGCATCTTGAACTTTATGATAACAAG
TCTTCACTTTCTTGTTTGCAAATTTCTTTCTAATTGTCAAAATATTCTGC
AGAAGTGCAAGAGCTCTTTACATTTTCTACTGCTTGCTGCACAAACAGAT
GGCCAATGAATTACATGCACCCCTATCTATAAATGCAGACCGTATATGCC
TCACCAGCAACAATAGGCAGAACTATCATAAAGACCTCAACAATGGCCGA
TTTTGCTTAAATTTCCGTCTTCCCTGCAGAATAATCTGATCGAATCAGGA
AGCAGCCTCGTGTCTTCTGCAAATGATAGAAGAAATGATTAGCACGGTAA
CAGACGGGTTACATTGGCAGACTAAGTACACATAGCTTGTAGTTGAGTTA
TAATAGCGGATACTATACTTCATATCAAATTGATAAACTCATTCTTGCAG
GATGTACCCAGAACAAGCAGGAGAGCAATAGAATAATGTTTGAAGAATAC
AAATACAAAACAATTCTATTACCACAAGCAATGAATAATTTACAAGGGGC
CGAAAAAACTTGACTGAAGACTCACTAATGCCAAGGACTAGCAGTACAGC
TTTATTAAACGCCTAACATGCGTAATGGACTAGAGCTTGTATTAGACATT
AAAGAGCTTATTTACAGTGTTTGGATCATTGATTAGTTTTCGAGTTAGGC
ACCAGTGGCCTAGAGAAGGACTATTATGACATATGATTTTCAATCAGATC
TGGAGCTTGCGCAACCATAGCACCACCAAGGCACATCATCTGCGGTCTCT
GAGTCCTCAATTAGTTCTCTGGCTTGTGCAGCCAAAACAGCAAAACCGAT
AAAATTCCCAATTCCCATGAGAAATAAACCGTAAGATATGTTTTCTGGAT
CGTATAGGAAGAGGAGACGACAGCAATGTATGTCGGTGATCACCAGAAAA
GAAGCTAGAGCTAACAATGCAGCATATCTGAAAGCTATCCAGTTCTTACT
CCATACTGCAACATTCCTGCAAAAACCATGATAGGTAGACAAGTTAGGAA
AGATCAATCACAATAACTAACACATAGTAACTTTGCGTTTGCTACTGCAA
CGTGTTACAATAATATATGATCCTGATAAGCCCTCTTCCTAACAGCTCGA
GATTTTAGGAGAATTGGTGACTTTACATGGTATCAATTTTCCCGCCACCT
CTTATTTGTAAAAATGCTGACCTCAAGAAATAAGATTATAGCCAACATTT
CTAAGTTCCTGCTGTGCATCTGACCTTTATAACTTGTATGAGTCTTTTTA
CGCGCTTATTCATATTCATGTTTTACCGTTTACTCCCCGCGGGAATTGAT
ACAACTCCAAATATTTATAATTTATTGAGCTTTTAATAGATCAAATATAG
ATTACTGATTCCGTACACGTAAGTATTTGAGTTTTGTTAAAAGAAATTAA
AAGAAAGAATTATTTCTCTTAAGTGCGAAACCTTTTATGAACTTTTTTAA
TTTTTAATTATAATATTTTAACGGTGATTTTGGATGTTGGGGATGGTCTG
TCAGTTAAGATGATGATATAGGAGTATGAGTTTTTTACAGAAAATTTGTT
CTTGAATGCATGTTTTTAATTCATGGATGGACCGGTTATCCATTGACTAA
CGATGATTAGTCACCAATGAGGCAATATGACACATACAATTTTGCACCCA
CAAATATTTGTCGAAGAATTTTTTGATAAATCAGAGATTTTTAATCTGAC
TTGAATATAATTAATAAATTATTTTTTAGAAGTTAATTATAATTAATCGT
TGATTCTTAATTTTTTTTGACAAGATCGCTGATTCTTGATTAAATCAAAA
TAACGGCAGAAACACCTAAATACCTAAATAGTCAAGTATAAATAGTTCGT
TTTTTTTATCAGGAGCATAATTCATCAATCTTGTACTAACGTTACGGGGA
AAATTTATCCATGAGTCGTTACTATTTATCATCCGTAATTTCGCCGGTCT
TTTAAGGTTTCCGTGTTCAATATTCAGTTTACTATTTCCTATCAAGTTGT
CTGCATCCAATTAGTCCGTCAACCGTTTTGTACTTATAGCAAAGAGACCT
TCGGGAGATTTTCGATCATGACTTGGTAATTGATTTCGAGAAGGAGAAGA
AAATGAGTAATAATATTATAATGCCTTAAAATTATATGAAAAGGTCTAAC
ATCTCTTTTGTCAAGCAAACGACTCATTCTCGACTCTCGCTATATTACTT
CTTTATGGTTGAAATTATTTCATTTTTTTTAAAAAAATATATGATAGATG
AATAATATTACATATTTCAAATTTTATTCTCAATTTTTTATAAATGACTT
GATAGACAGATGACTAATTTTAATTGGATGATTCATGTCAAAAAAAATTG
AAAACACTCGGTTAGTGCTGTAATTCGATCCGGGCAGCTCGCGAGCTCGC
CCTGCTCGAACTCGTTTTGATGGGCTCGGCTCGTTTAGTTAAATGAGCTC
GAGTTCGGGCTGAGGTTTTGGCTCGTTTCGTTAAACGAGCTGGCTCGATT
CGACTCGTGAAAATTAACGAGTCGGATTCGGATAGCGTTTTAGGCTCGAT
TTAGTGTTAAATCGGCTCGCCCGACTCGTTAAAATAGGCTCGTTTAGTTA
AACGAGCCAGCTCGACTCGACTCTTGAAAATAAACGAGTCGAGTTCGGGG
AAAGATTTAGGCTCGTTTTTTTAAACGAGCCGCATCAGCTCGACTAAATA
AAACTCGGCTCGAATTATGCTCGGCTAGCCCGGACCGAATAACAGCCCTA
CGCTCCGTGGTATCTGGCACTTGTTAAAAAATGAAGCTAGGAGGGAGTAT
ATGATCTTGTCAACGCCTGTCACTTTGCTGGGTTACTTGACACTTTAGAC
GCTTGGTCTGGGTGGTGCATTTGACTTTGTATTAAAGAATCCATGATATG
CTTACCTGTATTCGTTAGGATATTACAAGCCCAAAACAAGTTCAGAAGTG
ATCAGAATTGAGATCAAAACTCTCAACTCTCAAGTAAAAGCAAAACTCAT
GCAACATTTGATTAATACCGAGTCAATCCTAATCACTTTTAATTATTTTA
CTGCAACATAAATTTAAAATTGTTGTAAAAAAGGCCCATTTAATCACTGA
CTGCACATCTCACAGTTTGTTATCAAACGTTAGCTAACATTTGGAATCTC
TACTGGTAATAAAATCTGTTATTGCAATGGGCTTAAGTCTTGGTCGCATG
TTGGCTGCTTCTCTAGAGCTGATCGAAACCGGTAGATCTGCGATTTCTAA
ATATTCAGCTTCTAGTCCTTTTGTACTGATGTTAAGATAGTGCTCAATTC
TTGAAAACTTTTTGTTGACCAGGCTTAGGCTGGATTGAAGAGACGCGTGA
TCGCTATGTTAATTACTTAGTAATCTGGTTTTATTTGAACAAGGAGCAGA
TCTGGATCAAGGTATAATTTTTTCTCATTGAGATATTGATATATAATTGT
TTTACTTTGTTTTCTACATGTACCGGCGTGTTTCGTGTATCACGGGTCCT
TGGCTTTCCAATTTCTTTGCTGAATATGTTTATATGTGTTAAACTTGTTA
AACTAGTGAGTAATTGCAGAGAAACAATGGAGTTTACACACACAGAGAGA
GAGAGAGAGAGAGAGAGAGAGAGATTGAGATTGAGAGAGATGTAAAGAGA
GAAAGAGATCAGCTATCATTCATACACCTTAACTATACAATCTGGAAATG
ATCAAGACTATATACAGAAAGCCAAATGAAAATAAAGTGAGAAAGTTTGT
TATGAAACTAACTAATTAACTGATCAACTAAAACTAATGACAGCTATGTA
CAGATGGATAAATATTGTTGCGTAATTCCAACAATATGTTCATTCCTGTC
GTGATTGAATACAAATCGAATTGCCTGGACAACAGATCTGTGTGGCGTGA
GCAAGTGAAAACTTAGTTGCTTTTGTTGCTCTGAATTTTCAAGTAGTCGG
TTCTTCCTAAGATTAATACTATTTTGTCAAGCACTCGACGTGAAGTCATA
AAATAAAAAATTAAGGTTCAACCCTTCTCACAGTGATGATCACTGAATTG
CTTGCTGAATTAATGAATTGTTGTATTTGAATTAGATTTGAATTGCTTTG
AGCTGCTTAATTACAAAGCTTCATCCTTAAAAAAATACTTTGAGTACAAT
TGTGATTGAGATATTGGATTGAGTCCAGTTCTAGGCTCTAACTCAAAGCT
TATATCATTTTGTTTGACTGATTGATCTGTTGCAATAATAATTCATATTT
CTGGGTTGATTCATCAAATCAACCCACTGTCAATTCCATTGTAGTTTGCA
AACTTGCCTCGTCGTTTGCATTAGTTAAGACTTTAGATTGTAGTGTTTGT
TGGACCCTAGCCCTTGGTTTCTACGGTTCTTCAAGCGTTTAGATCATCGG
GTTTTATTGTTAGATGCCTTTGAGACATCTATAAATTTAACCAAATCTTG
GGAGCAGATTGCTATGGTTTGTTACCTCAAACCTATGCAGTTTGGCTCAT
GTCTCCATTTCAGAAAAAGAGTTTGTCACAAGCTGGTGTACTGTACATGT
CTACTGGCTACAAAAAGACTGTCGCGAGGCAATAGTCTGCTGTGTGCTGG
TTACATGTCCTTAGACAGAGTCATTCTCTGATCACACAATAGAGCAACGT
GCTTTTGGCAAGGGCCGATAAAGATGTTGTTGGTAATCGGTGGATGCAGA
GAGTATCATGAATGGGTGCAGTTACACGCAACATGATAACACTAAGGTGG
ACTTATGGCGAGTTTGCTCCTGGACTCTTGGTTTCAATGGCATTGCTCTG
AAGAGTGTTGTGTGTATGCAAAATTATGGTGACACTTGCAAGTTTTATTT
CAAATTGCTTTTTCATTCTTCGTAATGCTGTCAGAACTTGTTTCCAGATT
GGTTTTATGTCTGCGTTTTAATATGTTCTTTTCTGCAGTTCAAGAAACAC
CCCGTTGTGTGTCTGGTAGTTGTTCTTCAGCTGGCACTAATATTTAAGAA
TAGCCCTCTTTTAGGGTATGAAGTGGCTGTACCGAGGTGGGAAGCGACCT
GGATAGCAGGGATGTCAATGTTTTCCCCAGCCTTTCTCACTTACAAAATT
TTTGTGGAAATAAAAAAATTCAAGTTATCATGGAAGAAAAACAGGAAGGT
ATTCTGTGTAGGTATGTTCTTGGACACCTGAGGACCCATAAGCAAGAAAT
CGCATTTCCAACAATACTAATTGAAGTGGTGGTGCACATACTGTAAACGT
GAAGAATATCTAAGTGTTTGTTAATATATCTGTATATGTTGTTGATACAA
TTTGAAGTAGTGTGATTTTAGTAATTCGATGAGTCATACAGTTTGCACAA
TTGTGTTTTTTTGGGACTAGAACACATTGTTTGTACATAATACATGAAGT
TATAAACTTTATGAGTATAAAGATTGTACTGTCAGATTTTCCCACCTTGT
TTGGTTTTTACTTCTCTTGCACACTCAGATGCAAATGTATGGGAAGAGTC
TCAGAGGGACATGGAGAATCAATTATTTGCAAATTTAAATGCTTCAGAAG
AGTCTCACAGCAATTCGGAGACTCAATCACTTGCAGATGCCAATGTATCA
GAAGAGATGCATAGGGATGCAGGAAATCAATCTGGTGCAAATGAAAATGT
ATCGGAGGAGGTGCAGAGATACGGGGAACTAATCATTTGCAGATGCAAAT
TTATTGGTACAAACGCGGAGAATCAATTATTTGGAGATGCAAATGTGTTA
AGTGACCAATTCTCCTAAAACCTCAAACTGTTAGGGGGAGAGCTTACCAG
GATCATATTCTAACATTCCCCCTCACTCGAAAGCCCATTTATGGGTGGAG
TATTGCAGATGCTTTAG