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The mRNA, DCAR_001417, is a part of gene, DCAR_001417. |
The genetic_marker, MK001028 GSSR-029, is located in gene, DCAR_001417. |
Name | Description |
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An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |
Transcript Name | Identifier | Type | Location |
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DCAR_001417 | DCAR_001417.mRNA | mRNA | DCARv2_Chr1:14957285..14965621 |
ATGGTGGTGAAGGAGGAGAAGACCCAAATGGTTCACTCACCCATTCTCAC
TTACTTTTCCATGCTTTCCCTTCTTTCTTTGTGCCCCCCTTTTGTTATTT
TACTGTAAGTCCCTTTTCTCTGTATCTATATGTGTTCTATATGGCTTAAA
GTTTTGAACTTTTTGTGGGTTTGGCTTGTTTCAGATTGTTTATTGGTTTA
GATTAATGTAAGGCTTGAATCTTATTACAAATTTGTATATGTATGTACTT
TTGTGTGTGTACATGTCTTAAAGTTTTGATCTTTTTGTGGTTTAGCAGGT
TTTAGATTGTTTATTGGTTTTAGATCAATGTTGGGATTGAACCTTATCAC
AAATTGAATTTTTTTTGTTAAATTGTGTGTGTGTGTGTGTGTTTATGTGT
TTGATTTGGTCAATAAGTACAATAAATATGGAAGTAAGCTGGGTTGAAAA
TTAAAACACAACTAAATTGGTTGTGAATTTTGCGAAAGAATGGATCTTGA
TTTGCATCAGTCATTGCATATTAAATTCTTGAAATTTTGTTTTTTAAGGA
AGAATGAATCTGAATTTAGTGGTTTCCTGAAAGACAATGAAATTTGAAGT
GAATAATGAATTTTTTATTATCTAAAATGAAAATCATACTTATACAGTGC
ATATGATTGTATGCGATGCTTTATTATTTATTGAAGCCTTACTATATAAT
GAAATAAGAGAAAAATGAAGAAGGAATCTTATTTGTAAGAGAGCAATTTT
ATAAGCCTTTGGGATCATTGCTGCTTTTAGTTATTTCAAGTAGACTGTTT
AAATAAAGATAGCTGGGCATCATGTAGAATAATTTCAGAAGATCTTCTGT
TATCCTTGTGCATATTACAATACTCAAGGTGACAGATGGAACACTTGAAA
CTTAAAACTGTGAATATGCTCCAGGAAATTTTACACCTATATGAAACTGA
TTTTAATCTTTCATGGTTTTAGAGGTTCAGCTAATGATCAAAAAAGTACC
AAAAGCCTAATATTGTGCAGGGATGTATCTTTGAACAGTTCAAGCATCCC
GGTCATATTAAGTTCATTATTTTTTATCTGTTACAGTGCGGTTTAAATAA
GGTAATCTAATTTGAGTTCGATACTACATGTGCAGATGGTATACCATGGT
TCATGCTGATGGGTCTATATATCAGACATTTGATTTGTTCAGGCAGAATG
GGCTTCAGGCATTTTATGATATTTGGCCAAAACCCAGTATGACTGCGTGG
AAAATTATATTTTGTTATGGAGCTTTTGAGGCTGCACTTCAATTGTGGCT
ACCTGGAAAGAGAGTCGAAGGACCAATTTCTCCTGCTGGGAATAGACCTG
TCTATAAGGTAGATGAACAACAGATTCAGTAATGTAATTGATTTCATTTC
CAATGCATTAGTTACTAGTAGCTACACATCAACATTTGCAATTTTGTATA
TTCAATTTATTTAAAATTTATTTTTGCATTTGCTACTGAAATGAATATAC
AGTAATACCATTTTGCTAAAGATATCTAATTTCATAAAATTGAGGTCTTT
TGATGTCTACAACTTTTTTGGTTCTTTACTTAATTGTAGGCGAATGGCAT
GGCAGCATATGCAGTGACATTAATTACTTACCTTAGTCTTTGGTGGTAAG
GATTCTTCCCTTAACCATGTATTCAGATGATTAAGAAAGCTAGGATTCGT
ACATAGATGGTCTAAAGTTGCAAACCTTTATTCTATAAGTATGTAGTGAC
CTGGTATATGGTCTGCTGTTTTAGTTTTTCTGGGGATGATATAATAACTG
TTTACTGGTAAAAGAATACCCAGTATTGAAAAAACTTGTTTATTGTTCTG
TATCTTCTTGGACTTGCAGGAGAAAGTTGTCATTGTTTATTGTCTATATG
TATGGAAATTGGAAAGTATTCTTTACTCAAGTTTGCCCAAGCTAAAATAG
ATTATTGTTTTATTGTCAAATTCTTTGCTTCTTTGCCTAAACCATAAGCT
TATTATATCATTTCTAGCAATCGCCGACTTGTTCTCTATCCAACGCATAA
ATGGCTGAGATTTCACATTCTCAACCTCTTTGTTAAATCGGGCTCGAATT
AACAATGACGTTGATTTGCACAGATTACTTTTTCTTTTTGGGTGGGGTTG
GGGAAGAGGGGGGACTGAGCTTCCCCGTGTCTGTATAAGAAGGGACAAGT
TGATAGAGCATGTAACAGTATGTAGTTTAGACAGTTACAACTAGGATTAA
ACATGTTCCTCTGGCTTTGTGATTTTAGGTGTTAATGCTCTAGTATTTCG
GGAATATTTCCTGGATATCATAGAAATAAGGAACCTGCAGTAATACTACT
TCATGCTGCTTCACTTTAAGTAGGATCACATAAATTATTTATGCATTAGT
AAATGTCGGAGAAATCATGCTTAGTTTTGTCTGATGACGATTGGGAAGTG
GTCGGAAGTCATTTTCCTCTTTTCATTATTTATCTTCAGGCTTTGATAGT
TTAATTCTAACTGCCTTTACTGAATACTGGTTATTTGCTAAATGAGTGTT
TTCTTAACATTCATTATAATTATCAATTCAATGAACAACCTTAGGAGATA
CTGAGATAGGAACAAATCCCGATCGTCTGTATCTACATAAACATGTCAAC
TTCTGATTTCTGATTTCAGTATAGTAATATCAAGTGTCACACATAGTATT
TTTTTGAAGTATGCTCTAATTTGTGTACAGGTTTGGGATATTAAACCCTT
CCATTATCTATGATCATCTTGGAGAAATATATTCAGCCCTTATTTATGGC
AGCTTCATCTTTTGTATTTTGCTATACCTAAAGGTGAGATTAGATGCAGC
AGATATTTGACGTCCAGTCGTCTAGTATACATTTGCCATAGTAAATTATT
CGTATTTCTAATACTTGTGTTTCATCCCATACTAGGGTCATGTGGCACCA
TCGTCTACCGATTCTGGTTCATCAGGCAATGCAATAATTGATTTCTATTG
GGTGCGCATCCTTACTGGTCTATGTTCTCTTGTCGTCTGTCTGTTTCCTC
TTTCTTTATTCTAGAAAGACATTTATTGCTTCAATCAGTTTGTAAGTGCT
ATGTTAAATGAATTGATAGGTAAGCAAGAAATTCTGCGTATGAGGATGAA
CAACTATTGTTTATGATATTTTGAAGAAGATAAAACCTGTTATGTCTTGG
GAGCAATATATATGAGCTCCGAGCCTCTATGCATGTTTTTATTAATTATG
CTGCCACATGCAATCCTAAAATTGCTTGCCATGCTTCCAGTGAGCCCATA
TATGTGATAAAATCTTTCAACTTCTCTTACATTCTCCATTCTGAAATTTT
AATGAATTCTACATTGTATATTTAAATTCTCTACATGGGTTGTACTAATT
TGGAAGTGTTAGACCACTGGAATTTGTTAATTGTTCAATAAATCGAGAGG
TATCCAAGCGAGAAGCCCTGTGTAAGAGGTTGAACAAATATTTCTTGTTA
GATTTGAAGAAAATATAACCTTTTATGTTTTGGTAGCTATATATTTGACC
TCTGACCCTTTATGCATGTCATGTCTTTCATACCAGTGATGCAACTGTAA
AATTGCTCGTCATGCTAACAGTGAGCCCTTGTACACAAGAAAATCTTACA
TCTCTTACTTTCTCCATTATGAATTTTTGATGCACTCTCCATTGTAAATC
AAATTAATCTTCACGGGTTGTGGTCCTAATCAAAAAGTGTTGGATCTCAT
ATGATTGAATTTTTCTGTCCTTGCCATCCCCTTTTATTATGTTCATTAAA
GTGCTTTCTAGCATATATGATTTTCATGATGCCTGTAAAAGCATAAACAT
CAACTTGCGTTGCTTCTATCTACAAATTAAATGATATCTGAATCCTGAAA
TTACGGAGATGTATATTTTTCTAGCAAGCATCGCATGCATTAGAATGTGT
TGCAGACTACTTGATTATTCTTAAAACACCTACTATTCATCTGAAGATGC
ATAATGTGATTCATTTCTCCTGCTTATTATAGGGTATGGAGCTATATCCT
CGCATCGGCAAAAACTTTGATATCAAAGTGTTTACCAACTGCAGATTTGG
GATGATGTCTTGGGCGGTTCTTGCTTTGACTTATTGCATAAAGCAGGTAT
GTGCTCGAAGGCAATTTCTACTAGTTATATATGTTGCAAAGTGTATATGA
TACAATAACTTATCAAAATTCTTGTTAAATCTATGTGCATATAATTGGTT
GTGCACCACCCAAGCATCTTGCTATCTTGTACACCTGTTATACCAATAGT
TACATTCCCAAAAAAATTGTAACAATCTGGAAGTATAACATACTCAAATA
GGCTTCGAACTATTTTTTTCTGTCTTTACTCTCAGCAAGGGTATAATTGT
CAATAATTGACCTCTAAATATTCTAGTAGCTACTAGCTAGTAGATCTTTT
TCTGCTGGTAACTAAATTGAGTAGGATATCTCCTCCCAGAGAAGCCAGTA
GATTATGAATGGAGAATGGTTTACTTGTTTTCTATTTGATATATAAGCAA
TGAATGCATGCATTAAACATTTGCTGCATTTTTCTCTCTATAAAATGTCT
GATTCTATCCTTCCTCCCGATCTCTATTCTGAAAATTTTCCATCTTCTGT
TGCTTCTTTAGAATCTCGTCAGGCAAAGGTAAGCCTGAAAACTAAGTTTT
GTGTTCACTATGTAACAGTATGAAACAAATGGAAAAGCATCTGATTCAAT
GCTGGTAAACACTGCGCTAATGCTAATCTATATTACAAAATTTTTCTGGT
GGGAAGCTGGATACTGGAATACCATGGATATTGCACATGATCGAGGTATA
TATATGTATGCTTTAATCTTTCAGTTTAGTGTCTAGATCCAGTATTTGCT
ACTATGTCTGTTTCTAGAAGTAATACTAGTCTAGGGCTAGTTCTACTACT
TTTGAACACTCGATTATTTTAATTTCTTTATTTTACCATGAATTATATGT
TAAAAATTCACACCATATGGAGGACTGGTAATTTTTCTAGAAAATTCGGG
GACAAAATAATTTCTCTTGAATGGTTAAAGGATAAGAAATAGTCCAAAAC
AATATCTAAATGATTATGCGAGATTACAAGAGTTCTGGTCTTGCATAACC
TCTCTTATCCCAGCCCTTGCTTTTGGAGGCAAAATTGGCTTTGTCTGTTG
TTACTTACATTTTCCCTTTTTATTTCTGGACAGCCGGTTTTTATATATGT
TGGGGATGCTTGGTGTGGGTTCCATCTATCTACACTTCTCCGGGCATGTA
CCTTGTTAATCATCCCGTGAATCTTGGAATCCAAGTATGTAACTGGTCTG
GCACTGTTGATATGGTTCCAAAATGTTTAAAACCTAAGTTTTTTTTTTTT
TTACTTCTGTTCTTGCGCACCAAAAGTGTTGAAATTGCCCTTTTCCGGTT
GTCCTTGAGTCACTGTTGGATGATTATAACTTGAATAAAGGTGACAACTG
ACAAAATGGCGGTAGATAAAAATATTTCAACTAGATAGCTGGCTAGTGGC
AATGTTTAATTACTTGCCAGAGGGAATACTTTTGCGATTATTTAAATGTC
AGAGCCCTCTATAAATGAACTGAATTCCACCCCCTTCCCTCACAAACAAC
CTAAACGTGATAAATTAATAATAAGAAAATAAGTTACCGGGGTAAACCTG
TGAAAGTAATTTTTTCTTCTTCAAATTGCATGTATACACACAATTTAATG
ACATGAATTTTGAATTCACGAACTGACCAAAATTTTGACTTCCACGATGA
GACGAGTCATATATTTTGGAATGTTTGAAACACAAAGAAATATTTTGACT
GGAAAACTGTTTTGCATAGCACAATAAAATTTTAGTTGTCCCAGTAATTT
ATTTATGGCTCGTCATCTTATATTTTGGACCGTCATAGTCCCTTTTTAAA
TGTGTCAAATGTTTAGGCTGAGTTTGATACATGTAAATTGATTAGAATGA
GAAAACCAATTTTAAGAACTTACTTGATCTGATATGGACTGTGATTATAA
TAACCTAAGTGACCTGGGTGCCAAAGAGAGGCTAACAGATCTTCATTCTT
TCTACAGCTTTCTCTGTATATTCTTCTAGCAGGCATATTATGCATATATG
TCAATTATGATTGCGACAGGCAAAGGCAAGAGTTTCGTAGAACAAATGGG
AAGTGTGTGGTTTGGGGAAAAGCACCATCAAAGGTAACATATATGGAGGG
CAACTTGACTCGCACAAGATCACTTGCTGTGAAATATTGATTTTGTTTTC
TTCCATGTGAATTTTGTTACTCACTCTTGAAAAGGGATAATTTGTTCGGT
ATCTTACACAGATTACCGCTACATACACTACCAGTTCTGGTGAGACAAAA
ACCAGTCTTCTCCTGACTTCAGGATGGTGAGTATATATATATGCACCGCA
TTGTATCTGTCACACATAAATAAGGTCACATATGATTGAAGATACTAAAA
GTAATTTTTTTTATCGAAGAATAGTTTTTTATGTAATCTGGAAAGGAGTT
CTGTGTGAAGTAGGCACTATAATATATGTGCGAGTAACTAGCTTGCTACA
ATCTGTCTGTAATTTTTCTACGAGGAATTGGTAACTCTTCTTAAATTATA
TGGATCTTAAACAAGTAAACTAAGTAGTGAGAGGATAATTGTATATGAAT
AGTGTAAAAACAAACAATATCCTACAAGTTGTTGGTGAGGGATCTTTAAT
TCTCTAATCCCTAACTAGTAGATGCTAATGTAATTATTAGGTTAATACAA
TAATCTGTACTAAGACAAGACATAGGTATCTAAATATTATGTACAATCAA
GATTGCTTTGCTAAAGGACCTGTTGAAGAATCTACCTAGATAAAGTACGG
ATAATAGTAGAGTATGTAGTTGGACTTTTAATGGACCTCTGATAGATGCA
AACTATGTTCATAATCTCATCAACATGCTGTTATTAAGAATCTCATTTCA
ATATGAACAAGTTCAAATATATATTTGTTATTTCTGTTTTTTTTTCTAAG
AGATTATTGATGTTTACCATTATTAGCTAAAATAGAGTTGTCATTAATAA
GCGTCGTGCTTTTCTTGGTATTTAAAAGCAGGTGNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNGTTGTCATTAATAAGCGTCGTGTTTTTCTTGGTATT
TAAAAGCAGGTCTTATATTTGCCTAGTTTGAAGTTAAAGTATTTAGCATG
ATAACTATTGATTTAACCTGAACCTGACCAATTTATGTATCCATGAGGTG
ATTATAAATTTCTAATAAAGTATGAGTTTATTCATGCCCAGCACACTTTC
TGTGTTTTTCCCTTCAGGTGGGGCATATCTCGTCATTTTCATTACTTGCC
AGAAATATTGGCTGCCTTTTTTTGGACAGTACCAGCTCTTTTCAGCCATG
TAAGAATTAATTGCAATTTGAGAGAGAAGTTCTCCGTATTTTTTATACAG
GAGCATGCTAATACTATTTTTTTTCTTGCGATAATTCTTTTCTGCAGTTT
CTACCGTACTTTTATGTTGTCTTCCTTACCATCCTTCTTTTTGACCGAGC
CAAAAGAGATGATGATAGATGTCGGTCAAAGTAAGCAAATTTCTCATAAC
TCTAGTTTTTATCTAAAACTGTTGATACCTATCAGTTAATTAGCATAAGA
ACAGATAAATTGGAACAATCTTCTTCATCTTCTAGTAAAACAGCAATATT
ATCTCCCTATGATTCTACGCACATGATGATATAAGTTACCAAGTTGCAAA
TCGATCTTAAACAATTGGCAATGTTATTCACGGATCATATAACATCTAAA
ATTCATTTGATCAAACATCGGTAGACCGGAAGAAATGTGGTAAAGCTGGC
CACTTACTGTTTCTTCCTGGTGCATTATTTTGCAAACATCATTGTCCAAG
AATTGTGATTATATCGAACTGAGCTTTGTATTGGTAATATTAATGAACAT
CTCTCAAATCATATTGCAGGTACGGAAAATACTGGAAGTTATATTGCCAA
AAGGTTCCGTACAGGATCATACCAGGCATGTACTGA