XM_017363443.1

Resource Type: 
mRNA
Name: 
XM_017363443.1
Identifier: 
XM_017363443.1
Analysis: 
NameDescription

An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4.

There are a few different naming schemes for this assembly. First there is the
Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below.
These sequences can be viewed in JBrowse here.

Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0

LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926.

Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence.

RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records.

The NCBI Sequence report lists the correspondences between the various naming methods

Link to the LNRQ01000000.1 master record at NCBI

Raw Reads: Link to SRA accessions used for the genome assembly

This genome is available in the CarrotOmics Blast Search

The RefSeq genome records for Daucus carota subsp. sativus were annotated by the NCBI Eukaryotic Genome Annotation Pipeline, an automated pipeline that annotates genes, transcripts and proteins on draft and finished genome assemblies. This report presents statistics on the annotation products, the input data used in the pipeline and intermediate alignment results.

View the full report at https://www.ncbi.nlm.nih.gov/genome/annotation_euk/Daucus_carota_subsp._sativus/100/

Data from this analysis can be viewed in JBrowse here.

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Transcript Id: 
XM_017363443.1
Product: 
pyruvate, phosphate dikinase, chloroplastic, transcript variant X1
Sequence: 
CACAGTTTCATGTCTTGCAAGAAACACCATTTTTAATTGTTTCAAGAATC
AGTATATATATATTTATACCTGTACATATATCATCATTTGAAGCTGCACA
GTGAAGTGAAGTGAATGTCTTCATCTTATGGCAGCAGTATGCTTTCGAGG
TGTATTAGAAATACAGGGGATGTGTATACACAAAGATTGTTCAAGGAAAA
GTATGCAGACCAGCAGATTGATAGTGTTGGACCTTTACCTATTCCCGTGG
GGCAGAGGACGAGTCGAGTTCGACTCAGTTGCTGCCACTCGCCAAATAGT
CGTGAGACTCCAGCTCGGGGTCCAAACCGGTCTCAAGCTATTCTTACTCC
AATATCAGACCCTGCCAAGGTTTCCAAAAAGAGAGTATTCACATTTGGGA
AAGGAAATTGTGATGGCAACAAGGCTATGAAGTCTTTGCTTGGAGGCAAA
GGAGCAAATCTGGCTGAAATGGCAAGCATTGGTTTGTCAGTCCCTCCAGG
CTTCACTATTTCCACCGAAGCATGCCAGGAATATCAAAAAAATGGAAAGA
GTCTGCCACAAGGACTATGGGAAGAGATACTGGAAGGACTGGAGTTCGTG
CAGGAAGACATGGGTGCATTCCTTGGTGATTCATCTAAGCCTCTTCTCCT
TTCTGTTCGATCTGGTGCCGCAATATCGATGCCTGGTATGATGGACACTG
TCCTGAATCTAGGACTCAACGATGACGTAGTTGCTGGCTTGGCTTCCAAA
AGTGGAGAGCGCTTTGCGTATGATTCATATAGGCGTTTTCTTGACATGTT
TGGAAATGTTGTGATGGGAATTCCACACTCCTCTTTCGAGGAAGAACTAG
AAAATTTAAAAGCTGTGAAAGGGGTTAAGGAAGACACTGATCTAACTGCT
GCTGATCTTCAAGAACTTGTGGAGCAGTACAAGAAGGTCTACATCAAAGC
TACTGGTGGAAAGTTTCCTTCAGATCCAAAGAAACAGATGCAGTTGGCCA
TAAATGCAGTTTTTGACTCCTGGGACAGTCCAAGGGCCAACAAGTACAGA
AGCATCAATCAGATAACTGGATTAAAGGGAACGGCTGTGAATATTCAAAC
TATGGTGTTTGGGAACATGGGGAACACATCCGGTACAGGTGTCCTCTTCA
CAAGAAACCCAAGTACAGGTGAAAAGAAGCTCTATGGGGAGTTTCTAATC
AATGCTCAGGGAGAGGATGTGGTTGCTGGAATTAGAACACCAGAAGACCT
GAACACCATGAAAAATTGCATGCCTGATGCTTACAAGGAGCTCGTGGAGA
ATTGTGAAATCTTAGAGCGACACTATAAAGATATGATGGACATAGAATTC
ACTGTTCAAGAAAATAGGTTGTGGATGCTGCAATGTCGATCAGGCAAACG
CACAGGTCAAGGTGCAGTGAAAATTGCGGTAGACATGGTCAATGAAGGAC
TTGTTGATGCTCGCACTGCAATTAAGATGGTGGAGCCACAGCATCTGGAT
CAGCTTCTTCACCCGCAGTTTGAAAATCCTTCGGCTTACAAAGACAAAGT
GGTAGCCACGGGATTGCCTGCATCTCCAGGAGCCGCAATAGGGCAAGTTG
TATTTAGTGCTGAAGATGCTGAAGCTTGGCATGCACAAGGAAAAAGTGCC
ATCTTGGTAAGAACAGAAACTAGTCCAGAAGATGTTGGTGGTATGCATGC
TGCTGCAGGGATATTGACTGCTAGAGGGGGGATGACTTCTCATGCAGCTG
TCGTAGCCCGTGGCTGGGGAAAATGTTGTGTTTCTGGTTGTGCTGACATC
CGTGTGAATGATAGCGAAAAGTTGGTTGTGATTGGAGATTTGGTGATTTA
TGAAGGAGACTGGATCTCACTCAATGGTTCTACAGGTGAAGTGATAATGG
GAAAACAACCTCTTTCTCCTCCAGCTTTGAGTAGTGATTTAGAGACCTTC
ATGTCCTGGGCTGATGAAATAAGACAGCTCAAGGTTATGGCAAATGCTGA
TACGCCTGACGATGCATTGGCAGCACGGAAAAATGGTGCTGAAGGAATTG
GACTCTGTAGAACTGAGCACATGTTCTTTGCTTCTGATGAGAGGATAAAG
GCTGTAAGGAAGATGATAATGGCAGTTACACATGAGCAGAGGAAGGCAGC
CCTTGACTTGTTGTTGCCTTATCAAAGATCCGACTTTGAGGGCATTTTCC
GTGCAATGGATGGGCTTCCAGTGACAATCAGACTTTTGGACCCTCCACTT
CATGAATTTCTGCCAGAAGGTGACCTGGAAGAGATCGTTGGCGAACTAAC
TAGCGAAACTGGAATGAGTGAAGATGAAGTTTACTCGAGGATTGAGAAAT
TATCAGAAGTGAATCCCATGCTTGGCTTCCGTGGATGCAGGCTAGGAATT
TCTTACCCAGAGCTGACAGAAATGCAAGTGCGAGCAATTTTTCAGGCTTC
TTTGTCTCTGAGCAACCAGGGTTTTACAGTCTTTCCAGAGATTATGGTTC
CCCTCGTAGGAACTCCCCAGGAATTGAGACATCAAGTTAATCTTGTTCAC
ACTGTCGCAAAAACAGTATTCTCAGAGATGGGCTCATCCTTGAGCTACAA
GGTGGGCACCATGATAGAGATTCCTAGAGCTGCTCTTGTTGCGGATGAGA
TTGCAATGGAGGCAGAATTCTTCTCCTTTGGGACAAATGACCTCACACAG
ATGACATTTGGATATAGTAGAGATGATGTTGGCAAATTTCTTCCCATATA
CCTAGCCCAAGGCATTCTCCAGAGTGATCCATTTGAGGTACTTGACCAGA
AAGGTGTAGGTCAACTTGTCAAGATGGCAACTGAAAGAGGCCGAGCAGCT
AGGCCGAACTTGAAGGTTGGAATATGTGGGGAACATGGGGGAGAGCCATC
TTCCGTTGCTTTTTTTGCCCAGGCTGGGCTGGACTATGTCTCTTGTTCTC
CATTCCGGGTACCTATAGCGCGGCTAGCTGCAGCGCAAGTTGCTGTATAA
GAAGCAGTGTTTTGCAGTTTGGAATTGGGACTGAACTCTAAATTCGTCGA
AAATGTTGATCTTGAATTCTTGATCAGTTGTATATAAATAATCACGAATA
AACACATTCATCTAAAAACACACGTCATGCTGGATAGAAAAGAAACGGCA
AGAAAACTCTATCATAACATAAACAAACAGAGGATTGTGCATGGTTGTAA
ATTATCATCATTCAACTATAAGGCATGCTTGCGGAGCAACAGTATTGCTC
TCTGTACATGCTATGTGTGTTCTCTTAAACGCTAATAAATCAAAATGTGT
ATGTGTGTAATACTACGAATCTAGCTAATAAAAGTTATTTATAATTTTTC
ATTCTA
Sequence Length: 
3356
Sequence Checksum: 
345df0a402ef4b3eaca1682f2c0c1e8b
View location in JBrowse: 
Protein Sequence: 
MSSSYGSSMLSRCIRNTGDVYTQRLFKEKYADQQIDSVGPLPIPVGQRTS
RVRLSCCHSPNSRETPARGPNRSQAILTPISDPAKVSKKRVFTFGKGNCD
GNKAMKSLLGGKGANLAEMASIGLSVPPGFTISTEACQEYQKNGKSLPQG
LWEEILEGLEFVQEDMGAFLGDSSKPLLLSVRSGAAISMPGMMDTVLNLG
LNDDVVAGLASKSGERFAYDSYRRFLDMFGNVVMGIPHSSFEEELENLKA
VKGVKEDTDLTAADLQELVEQYKKVYIKATGGKFPSDPKKQMQLAINAVF
DSWDSPRANKYRSINQITGLKGTAVNIQTMVFGNMGNTSGTGVLFTRNPS
TGEKKLYGEFLINAQGEDVVAGIRTPEDLNTMKNCMPDAYKELVENCEIL
ERHYKDMMDIEFTVQENRLWMLQCRSGKRTGQGAVKIAVDMVNEGLVDAR
TAIKMVEPQHLDQLLHPQFENPSAYKDKVVATGLPASPGAAIGQVVFSAE
DAEAWHAQGKSAILVRTETSPEDVGGMHAAAGILTARGGMTSHAAVVARG
WGKCCVSGCADIRVNDSEKLVVIGDLVIYEGDWISLNGSTGEVIMGKQPL
SPPALSSDLETFMSWADEIRQLKVMANADTPDDALAARKNGAEGIGLCRT
EHMFFASDERIKAVRKMIMAVTHEQRKAALDLLLPYQRSDFEGIFRAMDG
LPVTIRLLDPPLHEFLPEGDLEEIVGELTSETGMSEDEVYSRIEKLSEVN
PMLGFRGCRLGISYPELTEMQVRAIFQASLSLSNQGFTVFPEIMVPLVGT
PQELRHQVNLVHTVAKTVFSEMGSSLSYKVGTMIEIPRAALVADEIAMEA
EFFSFGTNDLTQMTFGYSRDDVGKFLPIYLAQGILQSDPFEVLDQKGVGQ
LVKMATERGRAARPNLKVGICGEHGGEPSSVAFFAQAGLDYVSCSPFRVP
IARLAAAQVAV
Relationship: 
There are 2 relationships.
Relationships
The mRNA, XM_017363443.1, is a part of gene, LOC108196248.
The polypeptide, XM_017363443.1, derives from mRNA, XM_017363443.1.
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Cross Reference: 
NCBI GeneLoading content
NCBI NuccoreLoading content
Gene: 
LOC108196248
Model Evidence: 
Supporting evidence includes similarity to: 3 ESTs, 18 Proteins, and 100% coverage of the annotated genomic feature by RNAseq alignments, including 22 samples with support for all annotated introns
Gbkey: 
mRNA