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An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
This analysis was part of the carrot genome assembly publication In population 97837, root tissue was collected from plants with yellow (yyY2Y2) and white (YYY2Y2) genotypes, with two biological replications per genotype, at 80 days after planting (DAP). In population 70796, root tissue was collected from plants with dark orange (yyy2y2) and pale orange (YYy2y2) genotypes, with three biological replications per genotype, at 100 DAP. Total RNA was extracted from whole root tissue using the TRIzol® Plus RNA Purification Kit (Life Technologies, Carlsbad, CA) in accordance with the manufacturer’s protocol. RNA was treated for DNA contamination with the TurboDNA-free kit (Life Technologies, Carlsbad, CA). RNA quantity and integrity was confirmed with an Experion RNA StdSens Analysis kit (Bio-Rad, Hercules, CA). All samples had RQI values above 8.0. For each biological replicate, a 133 nt insert size paired-end library was prepared at the Biotechnology Center, UW-Madison (WI, USA). Libraries were sequenced on Illumina HiSeq2000 lanes using 2 × 100 nt reads. Reads were filtered using Trimmomatic version 0.32 with adapter trimming and using a sliding window of length ≥50 and quality ≥28, i.e. “ILLUMINACLIP:adapterfna:2:40:15 LEADING:28 TRAILING:28 SLIDINGWINDOW:10:28 MINLEN:50”. Filtered reads were aligned to the Daucus carota v2.0 genome assembly using the program TopHat v2.0.12 (ref. 30). Non-default parameters used were “--mate-inner-dist -67 --mate-std-dev 50 --min-intron-length 20 --max-intron-length 10000 --library-type fr-unstranded --num-threads 14”. The aligned read files were processed by Cufflinks v2.2.1 (ref. 61). Reads were assembled into transcripts with “cufflinks” using the carrot annotation v1.0 gene predictions as the reference gtf guide. Samples were combined with “cuffmerge”, and then differential expression analyzed with “cuffdiff”, using non-default parameters of “--multi-read-correct --min-alignment-count=5”. Using the abundance estimations, this performs tests for differential expression and regulation between the samples. Normalized counts of the mapped RNA sequences were used to calculate the relative abundances of transcripts expressed as Fragments Per Kilobase of exon per Million fragments mapped (FPKM). When testing for differential expression, biological replicates were included as a term in the mixed model analysis to account for experimental error. Testing for differential expression was done at the level of genes, isoforms, and promoters. Some of the data from this analysis were published in | |
This analysis was part of the carrot genome assembly publication In population 97837, root tissue was collected from plants with yellow (yyY2Y2) and white (YYY2Y2) genotypes, with two biological replications per genotype, at 80 days after planting (DAP). In population 70796, root tissue was collected from plants with dark orange (yyy2y2) and pale orange (YYy2y2) genotypes, with three biological replications per genotype, at 100 DAP. Total RNA was extracted from whole root tissue using the TRIzol® Plus RNA Purification Kit (Life Technologies, Carlsbad, CA) in accordance with the manufacturer’s protocol. RNA was treated for DNA contamination with the TurboDNA-free kit (Life Technologies, Carlsbad, CA). RNA quantity and integrity was confirmed with an Experion RNA StdSens Analysis kit (Bio-Rad, Hercules, CA). All samples had RQI values above 8.0. For each biological replicate, a 133 nt insert size paired-end library was prepared at the Biotechnology Center, UW-Madison (WI, USA). Libraries were sequenced on Illumina HiSeq2000 lanes using 2 × 100 nt reads. Reads were filtered using Trimmomatic version 0.32 with adapter trimming and using a sliding window of length ≥50 and quality ≥28, i.e. “ILLUMINACLIP:adapterfna:2:40:15 LEADING:28 TRAILING:28 SLIDINGWINDOW:10:28 MINLEN:50”. Filtered reads were aligned to the Daucus carota v2.0 genome assembly using the program TopHat v2.0.12 (ref. 30). Non-default parameters used were “--mate-inner-dist -67 --mate-std-dev 50 --min-intron-length 20 --max-intron-length 10000 --library-type fr-unstranded --num-threads 14”. The aligned read files were processed by Cufflinks v2.2.1 (ref. 61). Reads were assembled into transcripts with “cufflinks” using the carrot annotation v1.0 gene predictions as the reference gtf guide. Samples were combined with “cuffmerge”, and then differential expression analyzed with “cuffdiff”, using non-default parameters of “--multi-read-correct --min-alignment-count=5”. Using the abundance estimations, this performs tests for differential expression and regulation between the samples. Normalized counts of the mapped RNA sequences were used to calculate the relative abundances of transcripts expressed as Fragments Per Kilobase of exon per Million fragments mapped (FPKM). When testing for differential expression, biological replicates were included as a term in the mixed model analysis to account for experimental error. Testing for differential expression was done at the level of genes, isoforms, and promoters. Some of the data from this analysis were published in | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |
ATGAGCGCGGGAGTTTACAAATTCTGGAATTGTACACTATATCTGTTTCT
CATCTGCCATGCTCAAATCATCCCAACTTCTACTGCTACGGTAAAGGGAA
ACGAAACCGATCACCTAGCATTGTTGGCCTTCAAAGCAACAATTATCCAT
GATCCTCAAGGAGCTTTTAGCTCATGGAATATGTCTCTTAACTTCTGCAG
CTGGGCAGGCATAACATGCAGCAAACAGCACAAAAGAGTCACTAGCATAA
ACCTTGCATCAAAAGGTTTTGTGGGTTCTTTGCCGAGTGATATAGGAAAC
ATGAGTTTCCTGACTGAAATTGTTCTCACCAACAACAGTCTTCAAGGCAC
GATCCCTCAAGAAGTAGACCATCTTTTCAGGCTTAAAGTTCTAAGCTTAG
GACGAAATGCTTTGGAAGGAAATATACCAGATACCTTGGGACAAGTTAAT
AGGTTAGTCATTCTCGAACTTTTTTCCAATAACCTATCTGGTATGATCCC
CAATTCTGTTTTCAATCTCTCATCACTGAATGTCTTCAATTTGGCCAATA
ATCAACTTCAAGGAAGTATACCATCAGACTTTGGTCTGACCCATCACAAT
ATACAGAAGATTCAGCTATCTGATAACAGATTAAGTGGAAACATCCCTGT
ATCACTTTCTAATGCTTCAAAACTTCAAGTTATTCACTTGCAGTTCAACA
ATTTTAGTGGCCCCATATCAGTAGATTTTGGAAGGTTACTGTATCTTCAA
AATTTAAGTCTGGAATATAATAATTTCGGACTTGGGGAACAGGGTGACTT
GAAATTTATTGACTCGTTAGTAAATTGCAGGAGCTTAAAGATATTGAAAC
TAGGGGCCAATAACTTACAAGGATCATTGCCCCGTTCTATTGCAAATCTA
TCAATTAAGCTAACGATGATAAGCTTAGCAGATAACTGGATATCTGGAAG
CATCCCTCCTGACATATCCAAATTTATCAATTTGATCTTTTTATCTTTAG
AGCATAACAATTTCACGGGCATTATTCCCCCTGAAATCACCCAACTTGGA
AAGTTGCAACGCGTTCTTCTTTCTAACAACAGACTTACAGGGAACATTCC
GGCCTCCATAGGAAACCTATCTATGTTGGATGAAGTTCATTTAGAAAATA
ATGAATTGAACGGAACCATTCCTCCTAGTTTTGGAAATTGTCCAATGTTG
GTCCTCTTGGATTTGTCTCAAAATAACCTCAGTGGGACTATACCCAACGA
ATTTTTTCATGTTTCTCCTTTTTCTATCAAATTAAATCTTTCTCAAAACC
ATTTGGTTGGATCACTACCGGCAGGTATTGGTGGTTTGGACACCATAGTT
GAATTGGATATCTCTGAAAATGAATTTTCTGGTTTCTTTCCCACAGAGCT
TGGTAGATGCATTACCCTTGATACACTATACATGCAAGGCAACTTCTTTC
GGGGTCACATCTCCCACTCAATGCGAAATTTAAGAGGTATGCAAAATCTT
GATCTTTCTCGTAATAACTTATCCGGGGAAATCCCAGATTTTTTTGAGCT
GCCCTTGAAATATCTAAATCTAAGTTGGAATCATCTTGAGGGAGAAGTGC
ATACAAAAGGAGTATTCGCAAATGCAAGTGAATTTTCGATAGTAGGTAAC
AAAGAGCTCTGTGGAGGCATACCTGAACTGCGGCTACCAAGATGCAGTAG
TGACAGTGATAGATCACATAAACACAAAAGGTCCTGGGTACAAGATTTAT
TCATGATAGGTTTTATACTAGTTCTTCTCATTGTCGGTTACCATTTGTTG
CCTAGAGGGAAAAAAACTGAACCACCTGCTGTATCTATTAGACCTATATC
AGTATTACCCATCAGATTGTCATATAAGATTCTCCACCAAGCAACTGACG
GGTTCTCACGAGCCAACTTGGTCAGCGAGGGTGGTTTTGGGTCAGTGTAC
AAAGGAGAACTTGGTCCAGAGTACAATGGAAAAGCTGTTGCAATTAAAGT
ATTCAATCAAGAAGCAACCAACAGTTTCAGCACAGAGTGTGAAGCCCAAC
AACATATTCGCCACAGAAACATTGTTAAGATCAGAAGTACAAGTTCAAGC
ACTGCAAAGAAAGGCAAAATATTTAGAGCTATTGTTTACGACTTCATGGA
GAATGGGAGCTTAGATAGGTGGCAGCATTTAACATGTAGAACATCTCCTG
ATGAGCTTAGCATGCCTCAGATTCTTAACCTAAAGAACAGAATTAACATA
GCGATTGATGTGGCTAGTGCACTTGATTACCTTCATAATCAATTAGATTA
TCCCCTAATACATTGTAACCTAAGTCCAAGCAATATTTTCCTAGACACAG
AAATGAGCGCATATGTATCAAACTTTGGACTCGCAAAGTTTCTCATAGAT
CGAAGAATTTTTAATGGATTCGCAGGACCCTCCGAATATGCACCTCCAGA
GTACTACCAGGGAAGCATGGTGTCCACTAAGGGGGATGTCTACAGCTACG
GAATAATATTACTGGAGATGCTGACCGGGAAGAAGATTACTGGCCCTAGT
TTTCATGGATCGTTTAAGCTTCAGAACTTTGTTTCCAGTGTCTTGTCTAG
CGGGGTAAATGATGTTATTGACCCATTCAATCTACAAGAACTTAGTACAT
ATGATGCGGCAAAGGCAAATGATTGTCTAGTTATGCTTTTAGGCATTGGT
GTGCGATGTGCCCAGGAGTCACCAAAGTTCAGGCCGGATATTAAAGATAC
CTTAAGAGTTCTGGAAACAATTAGGAGCTTTTTCGAGGCCAATACAGGTT
GTACTCAGACGTATGCTATAGGAAGTTTGCAAGCTGCAGTCTCGATGGCT
CTAAGAAACAGGAGATCTCTACTTTGGAACAGGTTAGAAGTTTCAGAAAT
GCCCTCTCCAAATCTAGGGGAAACAAATTCAGGTAGAACCGACTGGAATG
TAGCCACAGCAAGGCAGTTGTTGGATCCTTTTCCTCAGATGGCTCCAAAT
TTAGCCACAGTGTCATACATGGATCTCCATAAAGCAACAAATGGGTTTTC
CTCAGCCAACTTAGTCGGTGCAGGTGGTTTTGGCTCTGTTTACAAAGGAA
CATTTAATCAAGGATATGTTCATCTTCTCTTAAGGGATAGTGAGATAGTA
GATGAGATTGGAATAGCTGTTGCAATCAAAGTATTTAACCTTCAACGTAG
AGGTGCAGTGAGAAGTTTCAACACTGAGTGTCAAATTTTAAACAACATTG
ACCACAAATATCTTGTAAAGCTTATAGCCACATGCACAAGTGTGGATCAA
GACGGTCACGATTTTCGAGCTATTCTTTTTGAGTTCATGGATCATGGGAA
GTATGGTCTAGGATGCAAGATGACAACCAAGGGAGACACATACAGCTTTG
GGATTCTATTACTGGAGATGCTGACAGGGAAGAAGCCTACTCATCGTATG
TTTCGGGGAGGTCTTACCCTTCACAACTTTGTTTCGCTGGCATTGCCTGA
CAATGTATTTAACATTACTGACACACTCATGAAGATTAAATGTTTTGCTT
CAGGCCAATACAGGTTGTACTCAGACGTATGCTATAGGAAGTTTGCAAGC
TGCAGTCTCGATGGCTCTAAGAAACAGGAGATCTCTACTTTGGAACAGTG
TTGTAGGTTAGAAGTTTCAGAAATGCCCTCTCCAAATCTAGGGGAAACAA
ATTCAGGTAGAACCGACTGGAATGTAGCCACAGCAAGGCAGTTGTTGGAT
CCTTTTCCTCAGATGGCTCCAAATTTAGCCACAGTGTCATACATGGATCT
CCATAAAGCAACAAATGGGTTTTCCTCAGCCAACTTAGTCGGTGCAGGTG
GTTTTGGCTCTGTTTACAAAGGAACATTTAATCAAGGATATGTTCATCTT
CTCTTAAGGGATAGTGAGATAGTAGATGAGATTGGAATAGCTGTTGCAAT
CAAAGTATTTAACCTTCAACGTAGAGGTGCAGTGAGAAGTTTCAACACTG
AGTGTCAAATTTTAAACAACATTGACCACAAATATCTTGTAAAGCTTATA
GCCACATGCACAAGTGTGGATCAAGACGGTCACGATTTTCGAGCTATTCT
TTTTGAGTTCATGGATCATGGGAAGTATGGTCTAGGATGCAAGATGACAA
CCAAGGGAGACACATACAGCTTTGGGATTCTATTACTGGAGATGCTGACA
GGGAAGAAGCCTACTCATCGTATGTTTCGGGGAGGTCTTACCCTTCACAA
CTTTGTTTCGCTGGCATTGCCTGACAATGTATTTAACATTACTGACACAC
TCATGAAGTCTACCACTAACTACATTGTTAATCTACATCTGTTAGTAGAA
TTTTCAGACAATTTTAGTCCAACCAAGTGCATGCACAATAATCTACACCA
ATCCTCGGCACTCAAAACATCTCACAGGATGAGAGCAGCAGGAGCTAACA
GATACTGGACTCATGTAATATCTTTGTTTCTCATCTCCACATCTCAAGCT
GCTGCATCAAGTGGAAACGAAACGGATCAACTAGCATTGTTAGCCTTCAA
AGCAAAGATTTCCCATGATCCCCTAGGAGCGTTTAGCTCCTGGAATATGT
CTCTCCATTTCTGCCTCTGGGAAGGCATTACATGCAGCCAACGCCACCAA
AGAGTCATCATCCTAGACCTCTCATCTAAAGGTCTGGTAGGTTCCTTGTC
TCCTCATATAGGAAACATGAGTTTTCTGACTAAACTTGTTCTCTACAACA
ACAATCTTCGAGGCTTGATTCCTCCAGAAGTAGACCGTCTTTCCAGGCTA
CAAGTCCTAAGCTTAGGCCGTAATGCTTTGGAAGGAAACATACCAGATAC
CTTGGGACGACTTAATAGGTTAGTCATTCTTGAACTTTTTTCCAGTAAGC
TATCTGGTATGATCACTACTTTTTTTAATCTCTCGTCACTGAAAGTCTAT
AATTTGGCCAATAATCAATTTCAAGGAAGTATACCATCAGAATTCGGATT
GGCCTATCCCAATCTACAAAAAATTCAGCTGTCTGATAACAATTTAAGTG
GAACCATTCCTGTATCACTATCTAATGCCTCCACACTTCAAGTTATTGAC
TTGCAGTTCAACCGTTTTAGCGGTCCCACACTTGTAGATTTTGGAAGGCT
ACCATATCTTCAGAAATTAGGCCTAGCAAATAATAATCTAGGAGTAGGGG
AACATGGTGACTTGAGTTTTTTCGACTCATTGGTCAACTGCAGCAGCTTA
AAGATACTGGAAGTAGGGGCCAATAATTTACAAGGATCATTGCCCTGTTC
TTTTGTCAATCTATCAACTGAGCTAACAATGATAAGCTTAGCAGATAATC
AGATTTTTGGAAGCATCCCTTCAGAGATATGTAAATTTACCAATTTGATC
TTTTTGTCATTAGGAGGTAACAAGTTCACAGGCATGATCCCCCCCGGAAT
TGTTAAGCTCAGGAAGCTTCAGCGAATTGTTCTTTCTAACAACAGACTTA
CAGGGAGCATTCCTGCCTCCATAGGGAACCTGTCTATGTTGGATGAAGTT
CATTTAGAAAATAATGAATTGAACGGAACCATTCCTTCGAGTTTTGGAAA
TTGTCCTATGTTGGTCCTCTTGGATTTGTCTCAAAATAACCTCAGTGGGA
CTATACCCAACCAATTTTTTCATGTTTCTCCTTTTTCTGTCAAATTGAAT
CTTTCTCAAAACCATTTGGTTGGATCACTACCAGCAGGTATTGGTGCTTT
GAAAACCTTGGTAAAGTTGGATGTTTCAGAAAATGAGCTGTCTGGCTTGA
TACCAACAGAGCTTTATGATTGCAGTGCCCTTGAATCACTCTACATGCGG
GGGAATTTTATTCAGGGTTTCAAGTCCCAACCAGTGAAAAAGTTAAGAGC
TCTGCAAAATATTGATCTTTCTCGTAATAACTTATCTGGAAAAATCCCAG
ATTTCTTTGAAACCCTACACTTGAAATATTTGAATCTTAGCTGGAATAAC
CTTGAGGGAGACGTACCTAAGAAAGGATTATTCGCAAATGCAAGTGCATA
TTCAATAGTAGGTAACAGGCTCTGTGGTGGGATACCTGAACTACAGCTAC
CAAGATGCAGTAGTTACAGTCCAGCTTATCAAATAATAGTGGTTCTACTT
GGGCTGGTTTTTATACTACTTATTTTCACTATTTTGTTACATATTTATGC
AAATATGGTGCTGCGTTCAGTATTAAATAGACCCATTTCAGTATCACCCA
TCACATTGTCATACAAGCAACTCCATCAAGCCACTAATGGTTTCTCAAAA
ACCAACTTAGTCAGCAAGGGTGCTTCCGGTTTAGTGTACAAAGGAAAAAT
TGGTCCACAATACAATAAAAGAGCTGTTGCAATTAAAGTTTTCAATGAAG
AAGCAACCAACTGTTTCATCACTGAGATTGAAGCCCTGCAGAATATTCAC
CACAGAAACATTGTTAAGATCATGAGTATATGCTCAACTATTGTAGAGAA
CCGCGGTTTTAAAGCTATTGTTTACAATGTCACTGAGCATAGGAGCTTGG
AGTGGTGGCTGCATTCAAAAAAGTACTGCCTGAGAAGCTCAATGTCAACC
AAGGGGGATGTGTACAGCTACGGACTAATTTTGCTGGAGATGCTGACAGG
CAAGAAGACTACTGACCCTATGTTTAACGAAGGTTATAAGCTGCAGAACT
TTGTCTCCAGTGCCTTGTCTGATGGTGTAAAGGATATTATTGACCCAGTC
AATCTACATGAACTTAGTAGAGATGCTGCAGCACATGCAGAAGACTCTCT
GAGTATGCTTTTCAACATTGGTGTGAAATGTACCCTGGAGATGCCACAGT
TTCGACCAGACATAAGGGATACCTTAAGCATGCTGAAAACAGTTCGGATC
AACTTTAAGGCTAATCATACTACTGAGGCATATGCTCGGACAAGCTCCCT
AGCTTCAGTCATTATGGCTCAAAGAAACATGAAATCTCTACCATTGTATG
GGATAACAAAAATGGCTCCAAACTCAAGATCAATGTCGACGTTATCATCA
GTCGTGGTGTCATATGTGGATCTCCACAAAGCAACTAATGGATTTTCCCT
TACTAATCTAGTCGGTGCAGGTGGCTATGGCTCTGTTTATAAAGGAAGAT
TTCTTTTAGGGAATAGTGGATTAGAAGACGGAACAGGAACAGCTGTTGCA
ATCAAAGTATTCAACCTTCAGCGTAGAGGTGCAGTGAAAAGTTTCAACAC
TGAATATGGAACCTTAAAAAACATTCACCACCCGAATCTTGTAAAGACTA
TAACTACTTGCACAAGTGTAGATAAGGAGGGTGGTGCTTTTCGAGCAATT
CTTTATGAGTTCATGGATCATGGAAAGTATGGCCTAGGATGCAAGATGAC
AACCAAGGGAGACACTTACAGCTTTGGGATTCTATTGCTGGAGATGCTGA
CAAGAATGAAGCCTACTCATCGTATGTTTCGTAAAGGTCTTAACCTTCAC
AACTTTGTTTGGATGGCCTTTCCTAACAACATTGTTGACATTACTGACCC
ACTTATGAAGATAAGATCATCTGCAAATGAAGGGGAATGCCTAACTAGAC
TGTTCAATATTGGAGAATTTTCAAACAAGCATCCACACCAGACCGCGGCA
CTCGAAACATCTTACAGGATGAGAGCAGCTGGAGTTAACAGATACTGGAC
TCATGTAATATCTTTGTTCCTCATCTCCACATCTGCTGCTGCTGCTGCAT
CTGGTGGAAATGAAACGGATCAACTAGCATTGTTGGCCTTCAAAGCAAAG
ATTTCCGATGATCCTCTAGGAGTGTTTAGCTCCTGGAATATGTCTCTCCA
TTTCTGCCTCTGGGAAGGTATTACATGCAACAAACAGCACCAAAGAGTCG
TCAGCCTAAACCTCTCCTCGACAGGTCTGGTAGGTTCTTTGTGTCCTGAT
ATAGGAAACATGAGTTTTCTGCGTGAAATCGATCTTTACAACAACAGTCT
TCAAGGCCCAATTCCTCAAGAAGTAGACCGTCTTTTCAGGCTAAAAGTTC
TAAGCTTAGGCCGTAATGATTTGGAAGGAAAGATACCAGGCACCATAGGA
CGTCTGAAAAGTTTATTCTTTGTCGATATCTCTTCAAATGAACTCTCTGG
TATGATCCCTACTTCAATTTTCAATATCTCGTCATTGTCTTCCTTCAATT
TGGCCAGTAATCAGCTTGAAGGAAGTATACCATCAGACATCGGATTGACT
CTTCCAAATCTGAAACTGATTCGGTTATCATATAACAAATTAACTGGAAG
CATTCCTGCATCGCTCTCTAATGTTTCCAAACTTCAAGCTATTGAATTCC
AGTCCAACAACTTAAGAGGCCCTGTATCTGTGGATTTTGGAAGATTGCCG
TATCTTCAATTACTACGCCTGTCGTATAATAATCTAGGATCTGGAGAAGA
GGGTAACTTGAGTTTTCTTGATCCACTCACCAATTGCAGCAGATTACTAA
TATTGGAACTAGAAAGCAATAATTTTCAAAGGTCATTGCCCAGTTCAATT
GCTAATCTATCAACTACGCTACAAATCATAAGCTTAGCATATAATCAGAT
TTCTGGAAGCATCCCTTCCGGGATAGGTAATTGTATCAGCTTGATTCTTT
TATCTTTACAAGGCAACAAGCTCACGGGCATTATTCCCTCTGAAATTGCT
AGGCTCGGGAAATTGCAACGAGTTATTCTTTCTAACAACATGCTTTCAGG
GAGCATTCCTGCATCCATTGGGAACTTATCTTTGCTGGACGAGATTCATT
TAGAAGATAATCAATTACAGGGAACAATTCCGGCTGGTTTTGGAAACTGT
CCAATGTTGGTTTTCTTGGATTTGTCTCGAAATAACCTCAGTGGAACTAT
ACCTCGTAACTTATTTTCTATTTCCCCATTTTCTGTGAAACTAAATCTTT
CTCGAAATCATTTAGTTGGATCACTACCAGCAGCTATTGGAGCTTTGGGA
ACTGTAGTTGAATTGGATGTCTCAGAAAATGAGTTTTCTGGTTTTATTCC
AGTAGAGCTTGGTCGATGCATCTCCCTCAGTTCACTATACATGCAAGGGA
ACTTTATTCAAGGTTACATCCCTGAATCATTGACAAATTTACGAGGTCTG
CAATATATTGATTTTTCTCGTAACAAATTATCTGAGAATATCCCCGATTT
CTTTGGAGACCTAATCTACGTGGAATATTTGAATCTGAGCTGCAATAATC
TCGCAGGAAAGGTGCCAATGATAGGGGTATTTTCAAATGCAAGTGCATTT
TCAATAGTAGGTAACAAAAAGCTATGTGGAGGGATACCAGAACTGCGGCT
CAAAAAATGCAGTAAAAAATATATTGGAACATATAACCAAATTATGGCTT
GGGGACAGGTTTTCATCTTGATAGGCTCTATACAAGATCTTCTTATCATC
AGTTGCGTATTTATGTCATGGCATAGAAGGAAAAGAAGGGAACAACTGAG
ATACTCAGCATCAAATGTAATGATATCCCCCAACAAAATGTCATATGAGC
TACTCCGTCAAGCAACCAAGGGATTTTCACGAACCAACTTAGTCAGCGAG
AGTGGTTTTGGGTTAGTGTATAAAGGAAAACTTGATCTTAAATACATTGG
GATACCTGAGGGGGAGAAAGCAGCTATAGCAGTAAAGATATTTAACACTT
TGAATGAAGAAGCGGCCAACTGTTTCATCACTGAGTGTGAAACCCTGAAA
AATATTCGCCACAGAAATATTGTTAGAATCATAAGTACATGTGAGAACCA
CAAATTTAAAGCTATTGTTTACGACTTCATGAAGAATGGGAGTTTAGAAG
GGTGGCTGCATTCAACAAAGTATGGTCTGGGAAGCATGGTGTCAACTAAG
GGGGATGTCTACAGCTATGGAATAATTTTACTGGAGATGCTGACAGGAAA
AAAGATAACTGACCCTATGTTTCAAAATGGTTTTAAGCTTCAGAACTTTG
TTTCCAATGCCTTGTCTGATGGTATAAAGGGAATTGTCAACCCAGTCAAT
CTATTTGAACTTACAAGAGAGAATGCAGCAGAGGCGGAGGATTGTCTAAG
TATACTTTTCAACATTGGTCTGAAATGTGCGCTGGACTTGCCACAGTTTC
GACCAGACATCAGTGATATCTTAAGCATGTTGGAAACAGTTAGGATCATC
TTCAAGATGCCTCCAGATTCAAGATCAATGTCGATATCACCATCGGTCAC
ACTGTCATTCATGGATATCCATAAAGCAACTAATGGACTGTCATCAACCA
ACTTACTAGGCGCAGGTGGTTTTGGCTCTGTTTATAAAGCAACATTTCAT
CAAGAGAATGTTCGTCTTCTCTTAAGGAATAGTGGGATAGAAGATAGGAT
TGGAACAGCTGTTGCAATTAAAGTATTTAACCTTCAGCGTAGAGGTGCAG
CAAGAAGTTTCAAGACCGAGTATAAAATCTTAAGAAACATTAGCCACATG
AATCTTGTAAAGGTTATAACTGCATGTACAAGTAAAGATCTGGAGGGCGA
TTCTGAACCTGTTAAGATGTTTGATAGAACCGCTAATTTAGCAAACAATC
AAATAATCAATTATAAATGTGATTCTTCAGAAAAGTGGTTGGTTTTGATT
GGAATTGCTCCTGGCTCACCAGAGATATCTCATAGGTATAGCTTGATCCA
TGTGATTACCAAGCTTGGACTTCTATACGTTTATGACCTCAAAACAGCTG
CAGCACTCAACAATCCTAAGCTAGCTGTCGATCTTTCCGGCGGAAACTTA
CCTGTGGCAGAGAATTTGGTTGGTTATACACCTCATGATTATCTGTTTCT
TCTGCAAACAATTCTTCGATCAGATTCTCGGGGGGCTGTTAAGTTTGCGC
TCATGATGGCTCACATGGAGGGAGGCTGTCCAGTTGATTACAACATCATA
ACCGACCTTTTCATCGAGGTTCCTACAATCACAATACTAAGTGGAAACGA
AAGAGATCAACAAGCATTATTGGCCTTCAGAGCAAAGATCTTTAATGATC
CCCTCGGAGTTTTTAGCTCATGGAATATGTCTCTCCACTTCTGCAGTTGG
GTAGGGATTACATGCAGTAAACAGCACAAAAGAGTCACCAGCATAAACCT
CTCATCTAAAGCTCTGGTAGGTTCTTTGTCTCCTGATGTAGGAAACATGA
GTTTTCTGAGTGAAATCATTCTCTACGACAACCATCTTCAAGGCGCAATT
CCTCAAGAAGTAGACCGGCTTTTCAGGCTAAAAGTTCTAAACTTAGGACA
AAATGCTTTTGAAGGAAGCATACCAAATACCTTGGGACGACTTAATAGGT
TGGTCATTCTCAAACTTTTTTCCAATAAACTCTCCGGTGTGATCCCAACT
TCTGTTTTCAATCTTTCGTCACTGAATGTCTTCAATTTGGCCAATAATCA
ACTTCAAGGAAGTATACCTTCAGGCTTTGGGTCGACTCTTCCCAATCTGC
AAAAGATTCAGTTATCAGATAACAAATTCAGTGGAAGCATTCCCATGTCA
CTTTATAATGCTTCTAAACTTCAAACTATTGATTTGCAGTTCAACAATTT
TAGTGGCCCCATATCTGTAGATTGTAGAAGCTTAAAGATATTGGAACTGG
GGGCCAACAATTTACAAGGATCACTATCCCGTTCTATTGCTAATCTATCA
GTTGAGCTAACAATGATAAGCTTAGCAGATAATCAGATCTCAGGAAGCAT
CCCTCCCGATATATCAAAATTTATCAACTTGATCTTTTTATCTTTAGAAG
GAAACAACTTCACCGGCATTATTCCCCCTGAAATCACCGAACTTGGAAAG
TTGCAACGTGTTCTTCTTTCTAACAATAGATTTTCAGGGAACATTCCAGC
CTCCATTGGGAACCTATCTATGTTGGATGAGATACATTTACAAAATAATG
ATTTGAACGGAATGATTCCTCCTAGTTTTGGAAATTGTCCAATGTTGGTC
CTCTTGGATTTGTCTCAAAATAACCTCAGCGGGACTATCCCTAACGAACT
TTTTCAGGTTACTCCTTTTTCTGTCAAATTAAATATTTCTCGAAACCATT
TGGTTGGATCACTACCGGCAGGTATTGGTGCTTTGAAAACCTTAGTTGAA
TTGGATATCTCTGAAAATGAGTTTGCTGGTTTCATTCCAGCAGAGCTTGG
CGACTGTGTCACCCTTGATTCACTATACATGCAAGGCAACTTCTTACAGG
GCAATATCTCCCAATCAATGAAAAAGTTGAGAGGTCTGCGGAATGTTGAT
CTTTCTCGCAATAATTTATCTGGGAAAATCCCAGATTTTTTTGAAAACCT
ATCCTTGAAATATCTAAATCTAAGCTGGAATCATTTTAAAGGAGAGTTAC
ATACAAAAGGAGTATTTGCAAATGCAAGTGCATTTTCAATAGTAGGCAAC
AAAGGGCTCTGTGGAGGCATACCTGAACTGCGGCTACGAAGATGCAGTAC
ACACAAAATGTCTTGGGTACAAATTTTAATCATGATAGGTTCTATACAAG
TTCTTCTCGTTGTCGGTTGCCTTTTGTGGCTTAGAAGGAAAAAAACTGAA
ACACGTGCAGTATCAATTGGACCTGCATCAGTATTTCTGATCAGATTGTC
ATATGAGCTTCTCCACCAAGCAACTGACGGGTTCTCACGGGCCAACTTGG
TCAGCGAGGGTGGTTTTGGGTCGGTGTACAAAGGAGAACTGGGTGCACAG
TACAGTGAAAACGCTGTTGCCATTAAAGTATTCAATGAAGGAGCAGCCAA
CAATTTCAACACGGAGTGTGGAGCCCTACAAAATATACGCCACAGAAACA
TTGTTAAGATCAGGAGTACATGCAAAAGCATCGAAAGAAGCGGCAAAGAG
TTTACAGCTATCATTTACGACTTTATGGAGAATGGAAGCTTAGATAGTCT
GGAGATGCTAACAGGGAAGAAGATTACTGACCCTATGTTTGATAGATCCT
ATAAGCTTCAGAACTTTGTTTCCAATGCTTTGTCGGACAGGGTAATTGAT
GTTATTGACCCATTCAATCTACATGAACTTAGTAGATATGATGCAGCAAT
GGCAATGGATTGTCTAGAGATGCTTTTAGACATTGGTGTGAGATGTGCCC
TGGAGTCACCGCAGTTTCGGCCGGACATTAAAGATACCTTGAGCATTCTG
GAAACAGTTAGAAGGGTTTTCGAGGACAATACAGGTGGTACTGAGACATA
TACTACAGGAAGTTTTCAAGATGCAGTCTTGGTGGCTCAAATAAATTCGA
GATCTCTACCATGGAACAGGTTAGAAGTTCCAGAAGTGCCCTCTCGAAAT
CTAGTAGAAAGAAATTCAGGTGGAGCCGACAGGAATGTAGCCGCAACAAG
GCAATCGATGGATCCTTCCCCTCAGATGGCTCCAAATTCAGTCACAGTGT
CATACACAAGGCAGTTGAGGGATCCTTCCCCTCAGATGGCTCCAAATTTA
GCCACAGTGTCATACATGGATCTCCATAAAGCAACTAATGGGTTTTCCTC
AGCCAACTTAGTCGGTGCAGGTGGTTTCGGTTCTGTTTACAAAGGAACAT
TTGATCAAGAATATGTTCGTCTTCTCTTAGGGGATAGTGGGATAGAAGAT
GAGATCGGAATAGCTATTGCAATCAAAGTATTTAACCTTCAACGTAGAGG
TGCAGTAAAAAGTTTCAACACCGAGTATCAAACTTTAAACGACATTGACC
ACACATATCTTGTCAAGCTTATAACTACATGCACAAGTGTGGATCAGGAT
GGTCACGACTTTCGAGCTATTCTTTTTGAGTTCATGGATCAAGGGAGTTT
AGAGATGTGGCTGCATCCAACATATAAAAAATATGGTCTAGGATGCAAGA
TGACAACCAAGGGAGACACATACAGCTTTGGGATTCTATTACTGGAGATG
CTGACAGGGAAGAAGCCTACTAATCGTATGTTTCGGGGAGGGCTTAACCT
TCACAATTTTGTTTCGCTGTCCTTACCTGACGACGTGATTAAAATTACTG
ACCCACTCATGAAGGTAATGACATCTGCAAATAAAGGGGATGACAAACGA
GTAGAGGATTGCCTGACTAGAATGTACAGTATTGGTCTAGCATGCTCCAA
GACATCAGCAAAGGACCGACCAGACATGCGTGATAACAGCGAGTTAGGGT
TTAAGCGAGAGCGAGAGCGAGGGTTTTGCCAGCAAGGAGGAAATCTCTTC
ATCACTGAATCTCAAGCTCGAGTACAAATCTCTTCGTTTTTTCAGGGTTT
TGATTTGTTGAAAATGGATGATGAGTTTAAAGTTCTAAGGTTTAATTGGA
GGGGTAAAATTGTTGATATTAGAGTGGAGATAGATAAGTGCACTTTGTTA
GATGTAGTGATGGATTACGAAGCTAAAGGGAAGGAGGAAGGGGTTAAGCT
TGATTATCATTTCCCAACATTTTATTATCGGTACAAGGATGATCAATTTC
AGTTAGTAGTTGACAAAGATATGATGACTATGTTTAAAAGATGTGCTGAG
AAAGCTTTGATTCCCATTGCTGTTGGAACAACCATAAAGCCAAGCCCCAT
TCACAAGTTGGTTCTTAATTTGAGGAGGAGTAATGAAGCAAAGCACCCCC
CTGCAGCTGCAATAGACAATGTAGTTGAAAATGTCCAGGAAAATGATGTT
GGACTTGAAGATTTGGAAGATAACTCAGTTGCCACTCTATATTTTCCGGC
GAGAATGGCCGGCGGTGGTCCTATCAATTCCGGTAGTGGAAAAGCTTATC
CCGGAGAGCTTACTACATATATTATGCTAACGTGCATTATTGCAGCCATG
GGTGCAGCGATGGGTGGTTTGATCTTCGGTTACGATATTGGAATTTCAGG
TGGTGTGACATCGATGGATCCCTTCTTGAAGAAGTTCTTTCCATCCCTGT
ACAGAAAACAAAAGGCTGATCATTCGCAGAACCAGTACTGTAAATTCGAC
AGCCAAATACTAACGATGTTTACATCATCGTTGTACTTGGCTGCTTTGTG
TTCCTCAGTGGTGGCTTCGTGGGTGACACGTAGATTGGGTCGGAAGCTAT
CTATGCTTTCCGGTGGTTCATTGTTTTGTGCAGGTGCTCTGATTAATGGC
TTTGCTCAGGACGTTGCTATGCTCATTATTGGTCGGATTTTGCTTGGTTT
TGGTATCGGATTCGCAAATCATGCAGTGCCACTCTACCTATCTGAAATGG
CTCCATACGAGTACAGAGGAGGGCTTAATATTATGTTCCAGTTATCAATC
ACTGTTGGTATCCTCGTGGCTAACGTCTTTAACTATGCCTTTGATAAGAT
CGAAGGTGGTTGGGGCTGGCGATTGAGTTTAGGAGGAGCTGTTGTCCCTG
CAGTTATCATGATTGCTGGATCTTTGTATCTCCCTGAAACTCCCAATTCA
TTGATTGAAAACGGGAAGCAAGATAAGGCCAAAAAACAATTAATTAAAAT
TCGCGGTGTTGATGATGTTGAGGAAGAGTTTAATGATCTTATTGCGGCTA
GTGAAGCCGAAGATCCCTGGAGAAACCTCTGGCAGCCAAAGTATAGGCCA
CAACTTGTAATGGGTATACTTATTCCATTCTTTCAGCAAGTTGCCGGAAT
TAATGTTATTATGTTTTATGCCCCTGTTTTGTTCAAGACTATTGGTTTTG
GGGGTGATGCATCTCTTGTGTCTGCTGTGATCACTGGCGGAGACGCGGAG
TTAATGTGA
MSAGVYKFWNCTLYLFLICHAQIIPTSTATVKGNETDHLALLAFKATIIH
DPQGAFSSWNMSLNFCSWAGITCSKQHKRVTSINLASKGFVGSLPSDIGN
MSFLTEIVLTNNSLQGTIPQEVDHLFRLKVLSLGRNALEGNIPDTLGQVN
RLVILELFSNNLSGMIPNSVFNLSSLNVFNLANNQLQGSIPSDFGLTHHN
IQKIQLSDNRLSGNIPVSLSNASKLQVIHLQFNNFSGPISVDFGRLLYLQ
NLSLEYNNFGLGEQGDLKFIDSLVNCRSLKILKLGANNLQGSLPRSIANL
SIKLTMISLADNWISGSIPPDISKFINLIFLSLEHNNFTGIIPPEITQLG
KLQRVLLSNNRLTGNIPASIGNLSMLDEVHLENNELNGTIPPSFGNCPML
VLLDLSQNNLSGTIPNEFFHVSPFSIKLNLSQNHLVGSLPAGIGGLDTIV
ELDISENEFSGFFPTELGRCITLDTLYMQGNFFRGHISHSMRNLRGMQNL
DLSRNNLSGEIPDFFELPLKYLNLSWNHLEGEVHTKGVFANASEFSIVGN
KELCGGIPELRLPRCSSDSDRSHKHKRSWVQDLFMIGFILVLLIVGYHLL
PRGKKTEPPAVSIRPISVLPIRLSYKILHQATDGFSRANLVSEGGFGSVY
KGELGPEYNGKAVAIKVFNQEATNSFSTECEAQQHIRHRNIVKIRSTSSS
TAKKGKIFRAIVYDFMENGSLDRWQHLTCRTSPDELSMPQILNLKNRINI
AIDVASALDYLHNQLDYPLIHCNLSPSNIFLDTEMSAYVSNFGLAKFLID
RRIFNGFAGPSEYAPPEYYQGSMVSTKGDVYSYGIILLEMLTGKKITGPS
FHGSFKLQNFVSSVLSSGVNDVIDPFNLQELSTYDAAKANDCLVMLLGIG
VRCAQESPKFRPDIKDTLRVLETIRSFFEANTGCTQTYAIGSLQAAVSMA
LRNRRSLLWNRLEVSEMPSPNLGETNSGRTDWNVATARQLLDPFPQMAPN
LATVSYMDLHKATNGFSSANLVGAGGFGSVYKGTFNQGYVHLLLRDSEIV
DEIGIAVAIKVFNLQRRGAVRSFNTECQILNNIDHKYLVKLIATCTSVDQ
DGHDFRAILFEFMDHGKYGLGCKMTTKGDTYSFGILLLEMLTGKKPTHRM
FRGGLTLHNFVSLALPDNVFNITDTLMKIKCFASGQYRLYSDVCYRKFAS
CSLDGSKKQEISTLEQCCRLEVSEMPSPNLGETNSGRTDWNVATARQLLD
PFPQMAPNLATVSYMDLHKATNGFSSANLVGAGGFGSVYKGTFNQGYVHL
LLRDSEIVDEIGIAVAIKVFNLQRRGAVRSFNTECQILNNIDHKYLVKLI
ATCTSVDQDGHDFRAILFEFMDHGKYGLGCKMTTKGDTYSFGILLLEMLT
GKKPTHRMFRGGLTLHNFVSLALPDNVFNITDTLMKSTTNYIVNLHLLVE
FSDNFSPTKCMHNNLHQSSALKTSHRMRAAGANRYWTHVISLFLISTSQA
AASSGNETDQLALLAFKAKISHDPLGAFSSWNMSLHFCLWEGITCSQRHQ
RVIILDLSSKGLVGSLSPHIGNMSFLTKLVLYNNNLRGLIPPEVDRLSRL
QVLSLGRNALEGNIPDTLGRLNRLVILELFSSKLSGMITTFFNLSSLKVY
NLANNQFQGSIPSEFGLAYPNLQKIQLSDNNLSGTIPVSLSNASTLQVID
LQFNRFSGPTLVDFGRLPYLQKLGLANNNLGVGEHGDLSFFDSLVNCSSL
KILEVGANNLQGSLPCSFVNLSTELTMISLADNQIFGSIPSEICKFTNLI
FLSLGGNKFTGMIPPGIVKLRKLQRIVLSNNRLTGSIPASIGNLSMLDEV
HLENNELNGTIPSSFGNCPMLVLLDLSQNNLSGTIPNQFFHVSPFSVKLN
LSQNHLVGSLPAGIGALKTLVKLDVSENELSGLIPTELYDCSALESLYMR
GNFIQGFKSQPVKKLRALQNIDLSRNNLSGKIPDFFETLHLKYLNLSWNN
LEGDVPKKGLFANASAYSIVGNRLCGGIPELQLPRCSSYSPAYQIIVVLL
GLVFILLIFTILLHIYANMVLRSVLNRPISVSPITLSYKQLHQATNGFSK
TNLVSKGASGLVYKGKIGPQYNKRAVAIKVFNEEATNCFITEIEALQNIH
HRNIVKIMSICSTIVENRGFKAIVYNVTEHRSLEWWLHSKKYCLRSSMST
KGDVYSYGLILLEMLTGKKTTDPMFNEGYKLQNFVSSALSDGVKDIIDPV
NLHELSRDAAAHAEDSLSMLFNIGVKCTLEMPQFRPDIRDTLSMLKTVRI
NFKANHTTEAYARTSSLASVIMAQRNMKSLPLYGITKMAPNSRSMSTLSS
VVVSYVDLHKATNGFSLTNLVGAGGYGSVYKGRFLLGNSGLEDGTGTAVA
IKVFNLQRRGAVKSFNTEYGTLKNIHHPNLVKTITTCTSVDKEGGAFRAI
LYEFMDHGKYGLGCKMTTKGDTYSFGILLLEMLTRMKPTHRMFRKGLNLH
NFVWMAFPNNIVDITDPLMKIRSSANEGECLTRLFNIGEFSNKHPHQTAA
LETSYRMRAAGVNRYWTHVISLFLISTSAAAAASGGNETDQLALLAFKAK
ISDDPLGVFSSWNMSLHFCLWEGITCNKQHQRVVSLNLSSTGLVGSLCPD
IGNMSFLREIDLYNNSLQGPIPQEVDRLFRLKVLSLGRNDLEGKIPGTIG
RLKSLFFVDISSNELSGMIPTSIFNISSLSSFNLASNQLEGSIPSDIGLT
LPNLKLIRLSYNKLTGSIPASLSNVSKLQAIEFQSNNLRGPVSVDFGRLP
YLQLLRLSYNNLGSGEEGNLSFLDPLTNCSRLLILELESNNFQRSLPSSI
ANLSTTLQIISLAYNQISGSIPSGIGNCISLILLSLQGNKLTGIIPSEIA
RLGKLQRVILSNNMLSGSIPASIGNLSLLDEIHLEDNQLQGTIPAGFGNC
PMLVFLDLSRNNLSGTIPRNLFSISPFSVKLNLSRNHLVGSLPAAIGALG
TVVELDVSENEFSGFIPVELGRCISLSSLYMQGNFIQGYIPESLTNLRGL
QYIDFSRNKLSENIPDFFGDLIYVEYLNLSCNNLAGKVPMIGVFSNASAF
SIVGNKKLCGGIPELRLKKCSKKYIGTYNQIMAWGQVFILIGSIQDLLII
SCVFMSWHRRKRREQLRYSASNVMISPNKMSYELLRQATKGFSRTNLVSE
SGFGLVYKGKLDLKYIGIPEGEKAAIAVKIFNTLNEEAANCFITECETLK
NIRHRNIVRIISTCENHKFKAIVYDFMKNGSLEGWLHSTKYGLGSMVSTK
GDVYSYGIILLEMLTGKKITDPMFQNGFKLQNFVSNALSDGIKGIVNPVN
LFELTRENAAEAEDCLSILFNIGLKCALDLPQFRPDISDILSMLETVRII
FKMPPDSRSMSISPSVTLSFMDIHKATNGLSSTNLLGAGGFGSVYKATFH
QENVRLLLRNSGIEDRIGTAVAIKVFNLQRRGAARSFKTEYKILRNISHM
NLVKVITACTSKDLEGDSEPVKMFDRTANLANNQIINYKCDSSEKWLVLI
GIAPGSPEISHRYSLIHVITKLGLLYVYDLKTAAALNNPKLAVDLSGGNL
PVAENLVGYTPHDYLFLLQTILRSDSRGAVKFALMMAHMEGGCPVDYNII
TDLFIEVPTITILSGNERDQQALLAFRAKIFNDPLGVFSSWNMSLHFCSW
VGITCSKQHKRVTSINLSSKALVGSLSPDVGNMSFLSEIILYDNHLQGAI
PQEVDRLFRLKVLNLGQNAFEGSIPNTLGRLNRLVILKLFSNKLSGVIPT
SVFNLSSLNVFNLANNQLQGSIPSGFGSTLPNLQKIQLSDNKFSGSIPMS
LYNASKLQTIDLQFNNFSGPISVDCRSLKILELGANNLQGSLSRSIANLS
VELTMISLADNQISGSIPPDISKFINLIFLSLEGNNFTGIIPPEITELGK
LQRVLLSNNRFSGNIPASIGNLSMLDEIHLQNNDLNGMIPPSFGNCPMLV
LLDLSQNNLSGTIPNELFQVTPFSVKLNISRNHLVGSLPAGIGALKTLVE
LDISENEFAGFIPAELGDCVTLDSLYMQGNFLQGNISQSMKKLRGLRNVD
LSRNNLSGKIPDFFENLSLKYLNLSWNHFKGELHTKGVFANASAFSIVGN
KGLCGGIPELRLRRCSTHKMSWVQILIMIGSIQVLLVVGCLLWLRRKKTE
TRAVSIGPASVFLIRLSYELLHQATDGFSRANLVSEGGFGSVYKGELGAQ
YSENAVAIKVFNEGAANNFNTECGALQNIRHRNIVKIRSTCKSIERSGKE
FTAIIYDFMENGSLDSLEMLTGKKITDPMFDRSYKLQNFVSNALSDRVID
VIDPFNLHELSRYDAAMAMDCLEMLLDIGVRCALESPQFRPDIKDTLSIL
ETVRRVFEDNTGGTETYTTGSFQDAVLVAQINSRSLPWNRLEVPEVPSRN
LVERNSGGADRNVAATRQSMDPSPQMAPNSVTVSYTRQLRDPSPQMAPNL
ATVSYMDLHKATNGFSSANLVGAGGFGSVYKGTFDQEYVRLLLGDSGIED
EIGIAIAIKVFNLQRRGAVKSFNTEYQTLNDIDHTYLVKLITTCTSVDQD
GHDFRAILFEFMDQGSLEMWLHPTYKKYGLGCKMTTKGDTYSFGILLLEM
LTGKKPTNRMFRGGLNLHNFVSLSLPDDVIKITDPLMKVMTSANKGDDKR
VEDCLTRMYSIGLACSKTSAKDRPDMRDNSELGFKRERERGFCQQGGNLF
ITESQARVQISSFFQGFDLLKMDDEFKVLRFNWRGKIVDIRVEIDKCTLL
DVVMDYEAKGKEEGVKLDYHFPTFYYRYKDDQFQLVVDKDMMTMFKRCAE
KALIPIAVGTTIKPSPIHKLVLNLRRSNEAKHPPAAAIDNVVENVQENDV
GLEDLEDNSVATLYFPARMAGGGPINSGSGKAYPGELTTYIMLTCIIAAM
GAAMGGLIFGYDIGISGGVTSMDPFLKKFFPSLYRKQKADHSQNQYCKFD
SQILTMFTSSLYLAALCSSVVASWVTRRLGRKLSMLSGGSLFCAGALING
FAQDVAMLIIGRILLGFGIGFANHAVPLYLSEMAPYEYRGGLNIMFQLSI
TVGILVANVFNYAFDKIEGGWGWRLSLGGAVVPAVIMIAGSLYLPETPNS
LIENGKQDKAKKQLIKIRGVDDVEEEFNDLIAASEAEDPWRNLWQPKYRP
QLVMGILIPFFQQVAGINVIMFYAPVLFKTIGFGGDASLVSAVITGGDAE
LM*
Relationships |
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The mRNA, DCAR_027544, is a part of gene, DCAR_027544. |
The polypeptide, DCAR_027544, derives from mRNA, DCAR_027544. |