Resource Type:
Polypeptide
Name:
DCAR_004575
Identifier:
DCAR_004575.mRNA-protein
Sequence:
MGSKVVDLFLVILVLSITRVVAAKAKVAGDECRTKRCSRHGPGIRFPFHL
KDRQPEHCGLPGFRVSCDGGETLLELQYLANTSLQGIQLFLSTGTTVDSI
NYKSQKIILSNFSFQPKINNVTLVSTSTSWTSAIVPPPFGGERSYTNINT
TFASCSSGVVGQLDLLLTSLSGQAFQVYYFDGLVGFDQPSITSCTKVFNS
SFPFYMLGGGTATLNWSAPNCGKCEAKGEYCKLTENSTSSNVGIADYSTV
CLSRGHGSIKAIAGIIPGATLTVLVLVVLLYYIIRSYRRKKYDALKIEMF
LRDYKAMKPTRYSYADIKKITRQFSNKLGQGGFGSVYKGHITKDIIVAVK
ILNNDPKANGEDFINEVGTIGRIYHVNVVRLVGYCADGCNRALVYEFQPN
NSLEKFTYSGQNHKNNFLGWEKLQEIAIGIAKGIEYLHEGCAQQILHFDI
KPHNILLDQNFTPKISDFGLAKLCSKDQSIVSMTMARGTIGYIAPEIFSR
NFGKASSKSDIYSFGMLLLEMVGARNNTTVENSTETYFPEWIYHRLEEGG
EVAIQIEKQEDSNIAKKLTIVGLWCIGWHPVDRPSIKHVIHMLERPECPA
MPPNPFGPSNVRSFKSDLEVISESE*
Sequence Length:
626
Sequence Checksum:
d51327a4282af6086c9747f44ee4c73e
View location in JBrowse:
Organism:
Relationship:
| Relationships |
|---|
| The polypeptide, DCAR_004575, derives from mRNA, DCAR_004575. |
Blast Results:
The following BLAST results are available for this feature:
BLAST of DCAR_004575 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
BLAST of DCAR_004575 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P6VYH8 ((rust resistance kinase Lr10-like {ECO:0000313|RefSeq:XP_027106957.1, ECO:0000313|RefSeq:XP_027106958.1}))
HSP 1 Score: 592.808 bits (1527), Expect = 0.000e+0
Identity = 317/627 (50.56%), Postives = 410/627 (65.39%), Query Frame = 0
Match: A0A6P6VYH8 ((rust resistance kinase Lr10-like {ECO:0000313|RefSeq:XP_027106957.1, ECO:0000313|RefSeq:XP_027106958.1}))
HSP 1 Score: 592.808 bits (1527), Expect = 0.000e+0
Identity = 317/627 (50.56%), Postives = 410/627 (65.39%), Query Frame = 0
Query: 5 VVDLFLVILVLSITRVVAAKAKVAGDECRTKRCSRHGPGIRFPFHLKDRQPEHCGLPGFRVSC-DGGETLLELQYLANTSLQGIQLFLSTGTTVDSINYKSQKIILSNFS-----FQPKINNVTLVSTSTSWTSAIVPPPFGGERSYTNINTTFASCSSGVVGQLDLLLTSLSGQAFQVYYFDGLVGFDQPSITSCTKVFNSSFPFYMLGGGTA--TLNWSAPNCGKCEAKGEYCKLTENSTSSNVGIADYSTVCLSRGHGSIKAI--AGIIPGATLTVLVLVVLLYYIIRSYRRKKYDALKIEMFLRDYKAMKPTRYSYADIKKITRQFSNKLGQGGFGSVYKGHITKDIIVAVKILNNDPKANGEDFINEVGTIGRIYHVNVVRLVGYCADGCNRALVYEFQPNNSLEKFTYSGQNHKNNFLGWEKLQEIAIGIAKGIEYLHEGCAQQILHFDIKPHNILLDQNFTPKISDFGLAKLCSKDQSIVSMTMARGTIGYIAPEIFSRNFGKASSKSDIYSFGMLLLEMVGARNNTTV---ENSTETYFPEWIYHRLEEGGEVAIQIEKQEDSNIAKKLTIVGLWCIGWHPVDRPSIKHVIHMLERPECPAMPPNPFGPSNVRSFKSDL 618
+V L +V L+ T K+ D C +RCS GP IRFPF LKDRQP+HCG PGF +SC ET+LE + SL + S ++ I+Y+S+ + ++ PK++ ++++ P + + + +F CSS + + LS ++QVY S+ SCTK+F Y L L+WSAPNCG CEA+ ++C+L ++S+S+ + CL G G +K I G++ G L VL+ + Y I RS + KK D LKI FL DYKA+KP+RYSY+DIKKIT FS KLG+GG+G+VYKG ++ ++ VAVK+LNN K GEDFINEVGTIG+I+HVN+VRLVGYCADG LVYEF PN+SLEKFT S ++ N LG EKL IA+GIAKGIEYLH+GC Q+ILHFDIKPHNILLDQNF PKISDFG AKLCSK+QS VSMT ARGT+GYIAPE+FSRNFGK S KSD+YSFGMLL+EMVG R N + +NS E YFPEWIY++L++G E+ IQI+ ++D AKKL I+GLWCI W+PVDRPS+K VI MLE E P+MP NPFG +N + ++ L
Sbjct: 1 MVILNVVRLIFLATLTALGSCKIH-DNCTVRRCSDQGPAIRFPFRLKDRQPQHCGFPGFELSCTQSQETVLENPFRVKASLNQTKPPFSVKFVINKIDYESRLLYVAKLDGCLPGLLPKLD----------LSASLFQIP-----DFNDYDFSFFKCSSKKWYMIPI--PCLSNSSYQVYATQSTDSILFASLVSCTKMFEIPSVSYALIEPQIPLVLSWSAPNCGYCEAEDKFCRLRKSSSSAGIQ-------CLENGTGPLKKIKVTGMVLGPALIVLIGIAS-YRIFRSKKLKKEDQLKIGQFLEDYKALKPSRYSYSDIKKITGNFSEKLGEGGYGTVYKGKLSNEVFVAVKVLNNS-KGGGEDFINEVGTIGQIHHVNIVRLVGYCADGYRGVLVYEFLPNHSLEKFTSS--ENERNLLGLEKLHNIALGIAKGIEYLHQGCEQRILHFDIKPHNILLDQNFNPKISDFGQAKLCSKEQSAVSMTAARGTMGYIAPEVFSRNFGKVSHKSDVYSFGMLLIEMVGGRKNIKIGGQDNSAEAYFPEWIYNKLDQGEEITIQIDNEDDKITAKKLMIIGLWCIQWYPVDRPSMKAVIQMLEAEEPPSMPRNPFGSTNTINERATL 598
BLAST of DCAR_004575 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5J4ZHM3 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
HSP 1 Score: 591.652 bits (1524), Expect = 0.000e+0
Identity = 322/615 (52.36%), Postives = 407/615 (66.18%), Query Frame = 0
Match: A0A5J4ZHM3 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
HSP 1 Score: 591.652 bits (1524), Expect = 0.000e+0
Identity = 322/615 (52.36%), Postives = 407/615 (66.18%), Query Frame = 0
Query: 30 DECRTKRCSRHGPGIRFPFHLKDRQPEHCGLPGFRVSC-DGGETLLELQYLANTSLQGIQLFLSTGTTVDSINYKSQKIILSNF--SFQPKINNVTLVSTSTSWTSAIVPPPFGGERSYTNINTTFASCSSGVVGQLDLL-LTSLSGQAFQVYYFDGLVGFDQPSITSCTKVFN-SSFPFYMLGGG-TATLNWSAPNCGKCEAKGEYCKLTENSTSSNVGIADYSTVCLSR---GHGSIKAIAGIIPGATLTVLVLVVLLYYIIRSYRRKKYDALKIEMFLRDYKAMKPTRYSYADIKKITRQFSNKLGQGGFGSVYKGHITKDIIVAVKILNNDPKANGEDFINEVGTIGRIYHVNVVRLVGYCADGCNRALVYEFQPNNSLEKFTYSGQNHKNNFLGWEKLQEIAIGIAKGIEYLHEGCAQQILHFDIKPHNILLDQNFTPKISDFGLAKLCSKDQSIVSMTMARGTIGYIAPEIFSRNFGKASSKSDIYSFGMLLLEMVGARNNTTV--ENSTETYFPEWIYHRLEEGGEVAIQIEKQEDSNIAKKLTIVGLWCIGWHPVDRPSIKHVIHMLE-RPECPAMPPNPFGPSNV---------RSFKSDLEVISE 623
DEC KRC + GP IR PF LKD P+HCG PGF +SC G+T++EL LS +V I+YK Q I + + +I N+ L ++ PF + +Y N T +CSS G +L L LSG +QVY + SC+K++N S P+ + L+WS P CGKCE++G+YC+ +N + + T C + G + G+I G +L L ++ LY + S++ KK D +KIE FL DY+A+KP RYSYAD+KKIT QF +KLGQGG+G+V+KG ++ DI+VAVK+LNN K NGE+FINEVG IGRI+H NVVRLVGYCADG RAL+YEF PN+SLEKFT S +N KN+ LGWEKLQ+IA+GIAKGIEYLH+GC Q+ILHFDIKP NILLD NF PKI+DFG AKLCSK+QS+VSMT+ARGT GYIAPE+FSRNFG S KSDIYSFGMLLLE+VG + N V +N++E YFPEWIY+ L++G E+ IQIEK+ D I KKLTIVGLWCI W+PVDRPS+K VI MLE + ++PPNPF SN RSF +L VISE
Sbjct: 22 DECTDKRCRQDGPAIRSPFRLKDSHPDHCGYPGFDLSCTQNGDTVIELP-------------LSVKASVKEIDYKFQLIHIYDLLGCLPQQIRNLNLSAS-----------PFQLQENYFNF--TLINCSSAKSGLYELTPLPCLSGPGYQVYAIPSGYYLYWLPLFSCSKMYNILSVPYQIFDQKYDLQLHWSIPVCGKCESQGKYCRWKDNGSK-------HETECFKQHAAGASKRLKVTGVILGPSLLALTIIFTLYCVHISHKLKKEDEVKIEKFLEDYRALKPARYSYADVKKITSQFKDKLGQGGYGTVFKGKLSNDILVAVKVLNNS-KGNGEEFINEVGIIGRIHHFNVVRLVGYCADGFKRALIYEFLPNDSLEKFT-SVENCKNHSLGWEKLQDIALGIAKGIEYLHQGCDQRILHFDIKPSNILLDHNFDPKIADFGQAKLCSKEQSVVSMTVARGTKGYIAPEVFSRNFGNVSYKSDIYSFGMLLLEIVGGKKNIDVRAQNTSEVYFPEWIYNCLDQGEELGIQIEKEGDMKIVKKLTIVGLWCIQWYPVDRPSMKIVIQMLEGNGDTLSLPPNPFANSNPMTSSAINHGRSFLRELSVISE 601
BLAST of DCAR_004575 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A068VAS2 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
HSP 1 Score: 589.341 bits (1518), Expect = 0.000e+0
Identity = 318/627 (50.72%), Postives = 411/627 (65.55%), Query Frame = 0
Match: A0A068VAS2 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
HSP 1 Score: 589.341 bits (1518), Expect = 0.000e+0
Identity = 318/627 (50.72%), Postives = 411/627 (65.55%), Query Frame = 0
Query: 5 VVDLFLVILVLSITRVVAAKAKVAGDECRTKRCSRHGPGIRFPFHLKDRQPEHCGLPGFRVSC-DGGETLLELQYLANTSLQGIQLFLSTGTTVDSINYKSQKIILSNFS-----FQPKINNVTLVSTSTSWTSAIVPPPFGGERSYTNINTTFASCSSGVVGQLDLLLTSLSGQAFQVYYFDGLVGFDQPSITSCTKVFNSSFPFYML--GGGTATLNWSAPNCGKCEAKGEYCKLTENSTSSNVGIADYSTVCLSRGHGSIKAI--AGIIPGATLTVLVLVVLLYYIIRSYRRKKYDALKIEMFLRDYKAMKPTRYSYADIKKITRQFSNKLGQGGFGSVYKGHITKDIIVAVKILNNDPKANGEDFINEVGTIGRIYHVNVVRLVGYCADGCNRALVYEFQPNNSLEKFTYSGQNHKNNFLGWEKLQEIAIGIAKGIEYLHEGCAQQILHFDIKPHNILLDQNFTPKISDFGLAKLCSKDQSIVSMTMARGTIGYIAPEIFSRNFGKASSKSDIYSFGMLLLEMVGARNNTTV---ENSTETYFPEWIYHRLEEGGEVAIQIEKQEDSNIAKKLTIVGLWCIGWHPVDRPSIKHVIHMLERPECPAMPPNPFGPSNVRSFKSDL 618
++++ +I + ++T + + K D C +RCS GP IRFPF LKD QP+HCG PGF +SC ET+LE + SL + S ++ I+Y+SQ + +S PK++ +S S + + + +F CSS + + + LS ++QVY V S+ SCTK+F FY L L+WSAPNCG CEA+G++C+L+ SS G+ CL G G +K I G++ G L VL+ + Y I RS + KK D LKI FL DYKA+KP+RYSY+DIKKIT FS KLG+GG+G+VYKG ++ ++ VAVK+LNN K GEDFINEVGTIG+I+H+N+VRLVGYCADG LVYEF PN+SLEKFT S ++ N LG EKL IA+G AKGIEYLH+GC Q+ILHFDIKPHNILLDQNF PKISDFG AKLCSK+QS VSMT ARGT+GYIAPE+FSRNFGK S KSD+YSFGMLL+EMVG R N + +NS E YFPEWIY++L++G E+ IQI+ ++D AKKL I+GLWCI W+PVDRPS+K VI MLE E P+MP NPFG +N + ++ L
Sbjct: 3 ILNVLRLIFLATLTALGSCKIH---DNCTMRRCSDRGPAIRFPFRLKDYQPQHCGFPGFELSCTQSQETVLENPFRVKASLNQTKPPFSVNFVINKIDYESQLLYVSKLDGCLPGLLPKLD----LSASLF------------QIQDFDDDFSFFKCSSKKWYAM-IPIPCLSNSSYQVYAIHSSVDLRDASLVSCTKMFEIPSLFYALIVPQIPLVLSWSAPNCGFCEAEGKFCRLS----SSLAGVQ-----CLENGTGPLKKIKVTGMVVGPALIVLIGIAS-YRIFRSKKLKKEDQLKIGQFLEDYKALKPSRYSYSDIKKITGNFSEKLGEGGYGTVYKGKLSNEVFVAVKVLNNS-KGGGEDFINEVGTIGQIHHINIVRLVGYCADGYRGVLVYEFLPNHSLEKFTSS--ENERNLLGLEKLHNIALGTAKGIEYLHQGCEQRILHFDIKPHNILLDQNFNPKISDFGQAKLCSKEQSAVSMTAARGTMGYIAPEVFSRNFGKVSHKSDVYSFGMLLIEMVGGRKNIKIGGQDNSAEAYFPEWIYNKLDQGEEITIQIDNEDDKITAKKLMIIGLWCIQWYPVDRPSMKAVIQMLEAEEPPSMPRNPFGSTNTINERATL 596
BLAST of DCAR_004575 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P6WH70 ((rust resistance kinase Lr10-like {ECO:0000313|RefSeq:XP_027113851.1}))
HSP 1 Score: 588.956 bits (1517), Expect = 0.000e+0
Identity = 318/627 (50.72%), Postives = 407/627 (64.91%), Query Frame = 0
Match: A0A6P6WH70 ((rust resistance kinase Lr10-like {ECO:0000313|RefSeq:XP_027113851.1}))
HSP 1 Score: 588.956 bits (1517), Expect = 0.000e+0
Identity = 318/627 (50.72%), Postives = 407/627 (64.91%), Query Frame = 0
Query: 5 VVDLFLVILVLSITRVVAAKAKVAGDECRTKRCSRHGPGIRFPFHLKDRQPEHCGLPGFRVSC-DGGETLLELQYLANTSLQGIQLFLSTGTTVDSINYKSQKIILSNFS-----FQPKINNVTLVSTSTSWTSAIVPPPFGGERSYTNINTTFASCSSGVVGQLDLLLTSLSGQAFQVYYFDGLVGFDQPSITSCTKVFNSSFPFYMLGGGT--ATLNWSAPNCGKCEAKGEYCKLTENSTSSNVGIADYSTVCLSRGHGSIKAI--AGIIPGATLTVLVLVVLLYYIIRSYRRKKYDALKIEMFLRDYKAMKPTRYSYADIKKITRQFSNKLGQGGFGSVYKGHITKDIIVAVKILNNDPKANGEDFINEVGTIGRIYHVNVVRLVGYCADGCNRALVYEFQPNNSLEKFTYSGQNHKNNFLGWEKLQEIAIGIAKGIEYLHEGCAQQILHFDIKPHNILLDQNFTPKISDFGLAKLCSKDQSIVSMTMARGTIGYIAPEIFSRNFGKASSKSDIYSFGMLLLEMVGARNNTTV---ENSTETYFPEWIYHRLEEGGEVAIQIEKQEDSNIAKKLTIVGLWCIGWHPVDRPSIKHVIHMLERPECPAMPPNPFGPSNVRSFKSDL 618
+V L +V L+ T K+ D C +RC GP IRFPF L+ RQP+HCG PGF +SC ET+LE + SL + S ++ I+Y+SQ + +S PK++ +S S + + + +F CSS + + + + LS ++QVY S+ SCTK+F FY L L+WSAPNCG CEA+G++C+L +SS+ GI C G G +K I G++ G L VL+ + Y + RS + KK D LKI FL DYKA+KP+RYSY+DIKKIT FS KLG+GG+G+VYKG ++ ++ VAVK+LNN K GEDFINEVGTIG+I+HVN+VRLVGYCADG LVYEF PN+SLEKFT S ++ N LG EKL IA+G AKGIEYLH+GC Q+ILHFDIKPHNILLDQNF PKISDFG AKLCSK+QS VSMT ARGT+GYIAPE+FSRNFGK S KSD+YSFGMLL+EMVG R N + +NS E YFPEWIY++L++G E+ IQI+ ++D AKKL I+GLWCI W+PVDRPS+K VI MLE E P+MP NPFG +N + ++ L
Sbjct: 1 MVILNVVRLIFLATLTALGSCKIH-DNCTVRRCGDQGPAIRFPFRLEGRQPQHCGFPGFELSCTQSQETVLENPFRVKASLNQTKPPFSVKFVINKIDYESQLLYVSKLDGCLPGLLPKLD----LSASLFQIQDV------------DDDFSFFKCSSKKLYGM-IPIPCLSNSSYQVYATQSSGDLRYASLVSCTKMFEIPSLFYALIEPQIPLVLSWSAPNCGYCEAEGKFCRL----SSSSAGIQ-----CFENGTGPLKKIKVTGLVVGPALIVLIGIA-SYRMFRSKKLKKEDQLKIGQFLEDYKALKPSRYSYSDIKKITGNFSEKLGEGGYGTVYKGKLSNEVFVAVKVLNNS-KGGGEDFINEVGTIGQIHHVNIVRLVGYCADGYRGVLVYEFLPNHSLEKFTSS--ENERNLLGLEKLHNIALGTAKGIEYLHQGCEQRILHFDIKPHNILLDQNFNPKISDFGQAKLCSKEQSAVSMTAARGTMGYIAPEVFSRNFGKVSHKSDVYSFGMLLIEMVGGRKNIKIGGQDNSAEAYFPEWIYNKLDQGEEITIQIDNEDDKITAKKLMIIGLWCIQWYPVDRPSMKAVIQMLEAEEPPSMPRNPFGSTNTINKRATL 596
BLAST of DCAR_004575 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P3ZS05 ((rust resistance kinase Lr10-like isoform X1 {ECO:0000313|RefSeq:XP_015878214.1}))
HSP 1 Score: 588.956 bits (1517), Expect = 0.000e+0
Identity = 322/619 (52.02%), Postives = 401/619 (64.78%), Query Frame = 0
Match: A0A6P3ZS05 ((rust resistance kinase Lr10-like isoform X1 {ECO:0000313|RefSeq:XP_015878214.1}))
HSP 1 Score: 588.956 bits (1517), Expect = 0.000e+0
Identity = 322/619 (52.02%), Postives = 401/619 (64.78%), Query Frame = 0
Query: 28 AGDECRTKRCSRHGPGIRFPFHLKDRQPEHCGLPGFRVSC-DGGETLLELQYLANTSLQGIQLFLSTGTTVDSINYKSQKIILS---NFSFQPKINNVTLVSTSTSWTSAIVPPPFGGERSYTNINTTFASCSSGVVGQLDLLLTSLSGQAFQVYYFDGLVGFDQPSITSCTKVFNSS---FPFYMLGGGTATLNWSAPNCGKCEAKGEYCKLTENSTSSNVGIADYSTVCLSRGHGSIKAIAGIIPGATLT--VLVLVVLLYYIIRSYRR-KKYDALKIEMFLRDYKAMKPTRYSYADIKKITRQFSNKLGQGGFGSVYKGHITKDIIVAVKILNNDPKANGEDFINEVGTIGRIYHVNVVRLVGYCADGCNRALVYEFQPNNSLEKFTYSGQNHKNNFLGWEKLQEIAIGIAKGIEYLHEGCAQQILHFDIKPHNILLDQNFTPKISDFGLAKLCSKDQSIVSMTMARGTIGYIAPEIFSRNFGKASSKSDIYSFGMLLLEMVGARNNT--TVENSTETYFPEWIYHRLEEGGEVAIQIEKQEDSNIAKKLTIVGLWCIGWHPVDRPSIKHVIHMLERPECPAMPPNPFGPSN---------VRSFKSDLEVISESE 625
A ++C+ RC + P IRFPF +KD+QP +CG PGF +SC D ET+LEL A S V I+Y+SQ+I + N + N+ L ++ PF + + T F +CS+ LD L+ LSG A+Q+Y D + + CTK++N+S F Y L W PNCG CEAKG C L +N T D L + G I + GATL VL L+V++ Y + S+ + +K + ++IE FL DYKA+KP+RYSYADIK+IT QF +KLGQG +G+V+KG +T D VAVKILNN K NGE+FINEVGT+GRI+HVNVVRLVG+CADG RALVYEF P+ SL+K Y N+FLGW KLQEIA GIAKGIEYLH+GC Q+ILHFDIKPHN+LLD NFTPKISDFGLAKLC+KDQS VSMT ARGT+GYIAPE+FSRNFG S KSD+YSFG+LLLEMVG R NT T +N +E Y+PEWIY+ LEEG ++ I IEK+ED+ IAK+L IVGLWCI WHPVDRPS+K V+ MLE E MPPNPFG + R +LE I+E E
Sbjct: 25 AQNQCKELRCGHNSPPIRFPFRVKDQQPNYCGYPGFDLSCSDKHETVLELPTPAKFS-------------VRKIDYESQEIEVYDPYNCLLGKLLKNLDLTNS-----------PFHVPKDIPSDYTLF-NCSTLDRKNLDGLVPCLSGSAYQIYMVDSYTSIEYLPLVYCTKMYNASNVPFSIYRSSYDNLLLKWYEPNCGPCEAKGNQCGLKKNGTKLETECLD-----LKKSKGDSDKI--VATGATLGAFVLSLLVIVAYRVYSFNKLEKENQVRIERFLEDYKALKPSRYSYADIKRITNQFKDKLGQGAYGTVFKGKLTADFFVAVKILNNS-KGNGEEFINEVGTMGRIHHVNVVRLVGFCADGFRRALVYEFLPSGSLQK--YISLPDSNSFLGWGKLQEIAFGIAKGIEYLHQGCDQRILHFDIKPHNVLLDNNFTPKISDFGLAKLCAKDQSTVSMTTARGTMGYIAPEVFSRNFGSVSYKSDVYSFGILLLEMVGGRKNTDVTADNISEIYYPEWIYNLLEEGEDLRIHIEKEEDAKIAKRLAIVGLWCIQWHPVDRPSMKLVVQMLESGESLIMPPNPFGSTGQTKSNASKPARRMNLELETIAEVE 608
BLAST of DCAR_004575 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P3ZFU1 ((rust resistance kinase Lr10-like isoform X2 {ECO:0000313|RefSeq:XP_015878215.1}))
HSP 1 Score: 585.104 bits (1507), Expect = 0.000e+0
Identity = 321/617 (52.03%), Postives = 399/617 (64.67%), Query Frame = 0
Match: A0A6P3ZFU1 ((rust resistance kinase Lr10-like isoform X2 {ECO:0000313|RefSeq:XP_015878215.1}))
HSP 1 Score: 585.104 bits (1507), Expect = 0.000e+0
Identity = 321/617 (52.03%), Postives = 399/617 (64.67%), Query Frame = 0
Query: 28 AGDECRTKRCSRHGPGIRFPFHLKDRQPEHCGLPGFRVSC-DGGETLLELQYLANTSLQGIQLFLSTGTTVDSINYKSQKIILS---NFSFQPKINNVTLVSTSTSWTSAIVPPPFGGERSYTNINTTFASCSSGVVGQLDLLLTSLSGQAFQVYYFDGLVGFDQPSITSCTKVFNSS---FPFYMLGGGTATLNWSAPNCGKCEAKGEYCKLTENSTSSNVGIADYSTVCLSRGHGSIKAIAGIIPGATLTVLVLVVLLYYIIRSYRR-KKYDALKIEMFLRDYKAMKPTRYSYADIKKITRQFSNKLGQGGFGSVYKGHITKDIIVAVKILNNDPKANGEDFINEVGTIGRIYHVNVVRLVGYCADGCNRALVYEFQPNNSLEKFTYSGQNHKNNFLGWEKLQEIAIGIAKGIEYLHEGCAQQILHFDIKPHNILLDQNFTPKISDFGLAKLCSKDQSIVSMTMARGTIGYIAPEIFSRNFGKASSKSDIYSFGMLLLEMVGARNNT--TVENSTETYFPEWIYHRLEEGGEVAIQIEKQEDSNIAKKLTIVGLWCIGWHPVDRPSIKHVIHMLERPECPAMPPNPFGPSN---------VRSFKSDLEVISESE 625
A ++C+ RC + P IRFPF +KD+QP +CG PGF +SC D ET+LEL A S V I+Y+SQ+I + N + N+ L ++ PF + + T F +CS+ LD L+ LSG A+Q+Y D + + CTK++N+S F Y L W PNCG CEAKG C L +N T T CL +K G GA VL L+V++ Y + S+ + +K + ++IE FL DYKA+KP+RYSYADIK+IT QF +KLGQG +G+V+KG +T D VAVKILNN K NGE+FINEVGT+GRI+HVNVVRLVG+CADG RALVYEF P+ SL+K Y N+FLGW KLQEIA GIAKGIEYLH+GC Q+ILHFDIKPHN+LLD NFTPKISDFGLAKLC+KDQS VSMT ARGT+GYIAPE+FSRNFG S KSD+YSFG+LLLEMVG R NT T +N +E Y+PEWIY+ LEEG ++ I IEK+ED+ IAK+L IVGLWCI WHPVDRPS+K V+ MLE E MPPNPFG + R +LE I+E E
Sbjct: 25 AQNQCKELRCGHNSPPIRFPFRVKDQQPNYCGYPGFDLSCSDKHETVLELPTPAKFS-------------VRKIDYESQEIEVYDPYNCLLGKLLKNLDLTNS-----------PFHVPKDIPSDYTLF-NCSTLDRKNLDGLVPCLSGSAYQIYMVDSYTSIEYLPLVYCTKMYNASNVPFSIYRSSYDNLLLKWYEPNCGPCEAKGNQCGLKKNGTK-------LETECLD-----LKKSKGATLGAF--VLSLLVIVAYRVYSFNKLEKENQVRIERFLEDYKALKPSRYSYADIKRITNQFKDKLGQGAYGTVFKGKLTADFFVAVKILNNS-KGNGEEFINEVGTMGRIHHVNVVRLVGFCADGFRRALVYEFLPSGSLQK--YISLPDSNSFLGWGKLQEIAFGIAKGIEYLHQGCDQRILHFDIKPHNVLLDNNFTPKISDFGLAKLCAKDQSTVSMTTARGTMGYIAPEVFSRNFGSVSYKSDVYSFGILLLEMVGGRKNTDVTADNISEIYYPEWIYNLLEEGEDLRIHIEKEEDAKIAKRLAIVGLWCIQWHPVDRPSMKLVVQMLESGESLIMPPNPFGSTGQTKSNASKPARRMNLELETIAEVE 599
BLAST of DCAR_004575 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5J5ARI6 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
HSP 1 Score: 583.178 bits (1502), Expect = 0.000e+0
Identity = 320/614 (52.12%), Postives = 403/614 (65.64%), Query Frame = 0
Match: A0A5J5ARI6 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
HSP 1 Score: 583.178 bits (1502), Expect = 0.000e+0
Identity = 320/614 (52.12%), Postives = 403/614 (65.64%), Query Frame = 0
Query: 30 DECRTKRCSRHGPGIRFPFHLKDRQPEHCGLPGFRVSC-DGGETLLELQYLANTSLQGIQLFLSTGTTVDSINYKSQKIILSNFS--FQPKINNVTLVSTSTSWTSAIVPPPFGGERSYTNINTTFASCSSGVVGQLDLLLTSLSGQAFQVYYFDGLVGFDQPSITSCTKVFN-SSFPFYMLGGG-TATLNWSAPNCGKCEAKGEYCKLTENSTSSNVGIADYSTVC---LSRGHGSIKAIAGIIPGATLTVLVLVVLLYYIIRSYRRKKYDALKIEMFLRDYKAMKPTRYSYADIKKITRQFSNKLGQGGFGSVYKGHITKDIIVAVKILNNDPKANGEDFINEVGTIGRIYHVNVVRLVGYCADGCNRALVYEFQPNNSLEKFTYSGQNHKNNFLGWEKLQEIAIGIAKGIEYLHEGCAQQILHFDIKPHNILLDQNFTPKISDFGLAKLCSKDQSIVSMTMARGTIGYIAPEIFSRNFGKASSKSDIYSFGMLLLEMVGARNNTTV--ENSTETYFPEWIYHRLEEGGEVAIQIEKQEDSNIAKKLTIVGLWCIGWHPVDRPSIKHVIHMLE-RPECPAMPPNPFGPSNV---------RSFKSDLEVISE 623
DEC KRC GP IRFPF L+DRQP++CG PGF +SC +TLL L + S +V I+YKSQ I+L +I N+ L S+ P FGGE +N T +C+S + + +QVY + SC+K+++ S P+ + LNWS P C CE++G+YC+ +NS+ Y C L+ G G+I G +L VL ++ L+ I S++ KK D +KIE FL+DYKA+KP RYSYAD+KKIT Q +KLGQGGFG+V+KG ++ DI+VAVK+LNN NGE+FINEVGTIGRI+HVNVVRLVGYCADG RAL+YEF PN SLEKFT S +N KN+ LGWEKLQ+IA+GIAKGIEYLH+GC Q+ILHFDIKP+NILLD NF PKI+DFG AKLCSK+QS+VSMT ARGT GYIAPE+FSRNFG S KSD+YSFGMLLLE+VG + N V +N+ + YFPEWIY+ L++G E+ IQI K+ D+ IAKKLTIVGLWCI W+PVDRPS+K V+ MLE E ++PPNPF SN R F ++L VISE
Sbjct: 22 DECMVKRCRHQGPAIRFPFRLRDRQPDYCGYPGFDLSCSQSNDTLLHLPF-------------SVKASVKEIDYKSQSILLDYLDGCLPQQILNLHLDSS---------PFRFGGEHY---VNYTLINCTS-------------TKRYYQVYAVRSDWDLYYWTSFSCSKMYDILSVPYSIFDQPYDFRLNWSKPECRFCESQGKYCRRKDNSSK-------YEIECFNQLTEGVSERLKDTGVILGPSLLVLGIIFTLHRIHISHKLKKEDDVKIENFLKDYKALKPARYSYADVKKITNQLKDKLGQGGFGTVFKGKLSNDILVAVKVLNNS-MGNGEEFINEVGTIGRIHHVNVVRLVGYCADGFKRALIYEFLPNGSLEKFT-SAENCKNHSLGWEKLQDIALGIAKGIEYLHQGCDQRILHFDIKPNNILLDHNFDPKIADFGQAKLCSKEQSVVSMTAARGTKGYIAPEVFSRNFGNVSYKSDVYSFGMLLLEIVGGKKNIDVRAQNTNQVYFPEWIYNHLDQGEELGIQIGKEGDTKIAKKLTIVGLWCIQWYPVDRPSMKFVVQMLEGDGETLSLPPNPFVSSNPTTSSATKHGRPFNNELSVISE 588
BLAST of DCAR_004575 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B7AJA7 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
HSP 1 Score: 581.252 bits (1497), Expect = 0.000e+0
Identity = 330/615 (53.66%), Postives = 410/615 (66.67%), Query Frame = 0
Match: A0A5B7AJA7 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
HSP 1 Score: 581.252 bits (1497), Expect = 0.000e+0
Identity = 330/615 (53.66%), Postives = 410/615 (66.67%), Query Frame = 0
Query: 30 DECRTKRCSR-HGPGIRFPFHLKDRQPEHCGLPGFRVSC-DGGETLLELQYLANTSLQGIQLFLSTGTTVDSINYKSQKIILSNFSFQPKINNVTLVSTSTSWTSAIVPPPFGGERSYTNINTTFASCSSGVVGQLDLL-LTSLSGQA-FQVYYFDGLVGFDQPSITSCTKVFN-SSFPFYMLGGG-TATLNWSAPNCGKCEAKGEYCKLTENSTSSNVGIADYSTVCLSRGHGSIKAIAGIIPGATLTVLVLVVLLYYIIRSYRRKKYDALKIEMFLRDYKAMKPTRYSYADIKKITRQFSNKLGQGGFGSVYKGHITKDIIVAVKIL-NNDPKANGEDFINEVGTIGRIYHVNVVRLVGYCADGCNRALVYEFQPNNSLEKFTYSGQNHKNNFLGWEKLQEIAIGIAKGIEYLHEGCAQQILHFDIKPHNILLDQNFTPKISDFGLAKLCSKDQSIVSMTMARGTIGYIAPEIFSRNFGKASSKSDIYSFGMLLLEMVGARNNTTV--ENSTETYFPEWIYHRLEEGGEVAIQIEKQEDSNIAKKLTIVGLWCIGWHPVDRPSIKHVIHMLE-RPECPAMPPNPFGPSNV---------RSFKSDLEVISESE 625
DEC CS+ HGP IRFPF LKD QP+HCG PGF +SC T+LEL LS V I+YKSQ I + + + ++ S S PF + Y +N T +CSS + L + LS A +QVY + SCTK++ SS P+ + LNWS P CG CEA+G+YC+L N+T D + +KA AG+I G +L L LV+ LY + RS + KK D +KIE FL DYKA+KP+RYSYADIKKIT QF +KLGQGG+G+V+KG ++ ++ VAVK+L NN+ K NGE+FINEVGTIGRI+HVNVVRLVGYCADG RALVYEF PN+SLEKF S +NHKN+ L WEKLQ+IA+GIAKGIEYLH+GC Q+ILHFDIKPHNILLDQN PKI+DFGLAKLCSK+QS+VSMT ARGT+GYIAPE+FSRNFG S K+DIYSFGMLLLEMVG + N V +N+++ YFPEW+Y+RL +G E+ IQIE++ED+ I KKLTIVGLWCI W+P+DRPSIK+ + MLE + MPPNPF +N R+ S+L ISE E
Sbjct: 23 DECMETWCSQDHGPAIRFPFRLKDHQPDHCGYPGFDLSCTQSNHTVLELP-------------LSVKVLVQEIDYKSQSIHIDDLYHGCLLRQFPNLTISAS--------PFQIQEKYI-LNFTLFNCSSTKRISVYLFPVICLSSPAGYQVYATSSSRFISDVPLMSCTKMYKISSVPYQIFYKEYDLQLNWSKPICGHCEAQGKYCRLKNNNTQDETECFDIPKQPHTGAWKKLKA-AGLILGVSLLAL-LVIALYRVHRSKKLKKEDEVKIENFLEDYKALKPSRYSYADIKKITNQFKDKLGQGGYGTVFKGKLSNEVHVAVKVLDNNNFKGNGEEFINEVGTIGRIHHVNVVRLVGYCADGFRRALVYEFLPNDSLEKFI-SSENHKNHSLRWEKLQDIALGIAKGIEYLHQGCDQRILHFDIKPHNILLDQNLNPKIADFGLAKLCSKEQSVVSMTTARGTMGYIAPEVFSRNFGNVSYKADIYSFGMLLLEMVGGKKNIDVRAQNTSQAYFPEWVYNRLNQGEELEIQIEEEEDTIIVKKLTIVGLWCIQWYPIDRPSIKNAVQMLEGDGDTLTMPPNPFASTNPTRTNATKHGRALNSELAAISELE 612
BLAST of DCAR_004575 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: F6H6L7 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
HSP 1 Score: 577.4 bits (1487), Expect = 0.000e+0
Identity = 313/625 (50.08%), Postives = 406/625 (64.96%), Query Frame = 0
Match: F6H6L7 (Protein kinase domain-containing protein {ECO:0000259|PROSITE:PS50011})
HSP 1 Score: 577.4 bits (1487), Expect = 0.000e+0
Identity = 313/625 (50.08%), Postives = 406/625 (64.96%), Query Frame = 0
Query: 22 AAKAKVAGDECRTKRCSRHGPGIRFPFHLKDRQPEHCGLPGFRVSC-DGGETLLELQYLANTSLQGIQLFLSTGTTVDSINYKSQKIILSN--FSFQPKINNVTLVSTSTSWTSAIVPPPFGGERSYTNINT-----TFASCSSGVVG-QLDLLLTSLSGQAFQ---VYYFDGLVGFDQPSITSCTKVFNSSFPFYMLGGGTATLNWSAPNCGKCEAKGEYCKLTENSTSSNVGIADYSTVCLSRGHGSIKAIAGIIPGATLTVLVLVVLLYYIIRSYRRKKYDALKIEMFLRDYKAMKPTRYSYADIKKITRQFSNKLGQGGFGSVYKGHITKDIIVAVKILNNDPKANGEDFINEVGTIGRIYHVNVVRLVGYCADGCNRALVYEFQPNNSLEKFTYSGQNHKNNFLGWEKLQEIAIGIAKGIEYLHEGCAQQILHFDIKPHNILLDQNFTPKISDFGLAKLCSKDQSIVSMTMARGTIGYIAPEIFSRNFGKASSKSDIYSFGMLLLEMVGARNNTTVENST-ETYFPEWIYHRLEEGGEVAIQIEKQEDSNIAKKLTIVGLWCIGWHPVDRPSIKHVIHMLERP-ECPAMPPNPFGP-----SNVR----SFKSDLEVISE 623
+A+ DEC+ CS HGP I+FPF KD QP HCG GF +SC + +T+LEL Y S +VD INYKS++I++ + F Q ++ N+TL ++ PF + +N + TF SCSS + L + VY + D ++SC K++N S P ++ G +L WS CG CE G+ C+L NS T C+ K + G+I G + V V+++L + + RS K+ + ++IE FL DYKA+KP+RYSY DIKK+T F +KLGQGG+G+VYKG ++ +++VA+KILN D K NGE+FINEVGTIGRI+HVNVVRLVG+CADG RAL+YEF PN SLEKF +S + LGWEKLQ+IAIGIAKGIEYLH+GC Q+ILHFDIKPHNILLD+NF PKISDFGLAKLCSKDQS VSMT+ARGT+GYIAPE+ SRNFG AS KSD+YSFGMLLLEMVG R N V T + YFPEW+Y++L++G +V I+IE++ D IAKKLTIVGLWCI W+P+DRPS+K V+ MLER + +PPNPF N+R S + +L +ISE
Sbjct: 13 SAEIGANQDECKVPSCSPHGPAIKFPFRRKD-QPYHCGYLGFEISCTEKKQTILELPY-------------SVKLSVDKINYKSREIVVHDPDFCLQRQLQNLTLSAS-----------PFQFKVDSSNFESWTDDYTFFSCSSDTREYEFPFTLIPCRSRFLHENPVYAVRSFMQLDWLDLSSCHKLYNLSLPDGIVDGKKFSLTWSESICGNCEKAGQKCRLKSNSKGP-------ETECIP------KQVKGVILGFFVLVFVIIIL-HRVYRSNESKRENIVRIEKFLEDYKALKPSRYSYNDIKKLTNHFKDKLGQGGYGTVYKGKLSNEVLVAIKILN-DSKGNGEEFINEVGTIGRIHHVNVVRLVGFCADGVKRALIYEFLPNESLEKFIFSTSVETYS-LGWEKLQDIAIGIAKGIEYLHQGCDQRILHFDIKPHNILLDENFNPKISDFGLAKLCSKDQSAVSMTVARGTMGYIAPEVLSRNFGNASYKSDVYSFGMLLLEMVGGRKNIDVTMETNQVYFPEWVYNQLDQGEDVHIRIEEEGDIKIAKKLTIVGLWCIQWYPIDRPSMKVVVQMLEREGDNLTIPPNPFASIGSTGGNIRRRKKSLQQELTIISE 596
BLAST of DCAR_004575 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A438ICV5 ((Rust resistance kinase Lr10 {ECO:0000313|EMBL:RVW94538.1}))
HSP 1 Score: 577.4 bits (1487), Expect = 0.000e+0
Identity = 310/618 (50.16%), Postives = 405/618 (65.53%), Query Frame = 0
Match: A0A438ICV5 ((Rust resistance kinase Lr10 {ECO:0000313|EMBL:RVW94538.1}))
HSP 1 Score: 577.4 bits (1487), Expect = 0.000e+0
Identity = 310/618 (50.16%), Postives = 405/618 (65.53%), Query Frame = 0
Query: 22 AAKAKVAGDECRTKRCSRHGPGIRFPFHLKDRQPEHCGLPGFRVSC-DGGETLLELQYLANTSLQGIQLFLSTGTTVDSINYKSQKIILSN--FSFQPKINNVTLVSTSTSWTSAIVPPPFGGERSYTNINTTFASCSSGVVGQLDLL-LTSLSGQAFQVYYFDGLVGFDQPSITSCTKVFNSSFPFYMLGGG-TATLNWSAPNCGKCEAKGEYCKLTENSTSSNVGIADYSTVCLSRGHGSIKAIAGIIPGATLTVLVLVVLLYYIIRSYRRKKYDALKIEMFLRDYKAMKPTRYSYADIKKITRQFSNKLGQGGFGSVYKGHITKDIIVAVKILNNDPKANGEDFINEVGTIGRIYHVNVVRLVGYCADGCNRALVYEFQPNNSLEKFTYSGQNHKNNFLGWEKLQEIAIGIAKGIEYLHEGCAQQILHFDIKPHNILLDQNFTPKISDFGLAKLCSKDQSIVSMTMARGTIGYIAPEIFSRNFGKASSKSDIYSFGMLLLEMVGARNNTTVENST-ETYFPEWIYHRLEEGGEVAIQIEKQEDSNIAKKLTIVGLWCIGWHPVDRPSIKHVIHMLERP-ECPAMPPNPFGP---------SNVRSFKSDLEVISE 623
+A+ DEC+ CS HGP I+FPF KD QP HCG PGF +SC + +T+LEL Y S +VD INYKS++I++ + F Q ++ N+TL +++ ++P R Y+ + TF SCS + + + VY D+ ++SC K+++ S P+ M G WS C CE G+ C+L NS S T C+ + G+I G + V V+++L + + RS K+ + ++IE FL DYKA+KP+RYSY DIKK+T F +KLGQGG+G+VYKG ++ +++VA+KILN D K NGE+FINEVGTIGRI+HVNVVRLVG+CADG RAL+YEF PN SLEKF +S + ++ LGWEKLQ+IAIGIAKGIEYLH+GC Q+ILHFDIKPHNILLD+NF PKISDFGLAKLCSKDQS VSMT+ARGT+GYIAPE+ SRNFG AS KSD+YSFGMLLLEMVG R N V T + YFPEW+Y++L++G +V I+IE++ D IAKKLTIVGLWCI W+PVDRPS+K V+ MLER + +PPNPF RS + +L +ISE
Sbjct: 13 SAQIGANQDECKVPSCSPHGPAIKFPFRRKD-QPYHCGYPGFVISCTEKNQTILELPY-------------SVKLSVDQINYKSREIVVHDPEFCLQRQLQNLTL----SAYPFQLIPDSSNSLRVYSIHDFTFFSCSPNKEYRYPFMSIPCRFPHGNPVYAVSSNSYLDELDLSSCHKLYSLSLPYSMFNGEYNFYFTWSESICRNCEKTGKNCRLKSNSNSKRP-----ETECIP------IPVKGVILGFFVLVFVIIIL-HRVYRSNESKRENIVRIEKFLEDYKALKPSRYSYNDIKKLTNHFKDKLGQGGYGTVYKGKLSNEVLVAIKILN-DSKGNGEEFINEVGTIGRIHHVNVVRLVGFCADGVKRALIYEFLPNESLEKFIFSTSVNTHS-LGWEKLQDIAIGIAKGIEYLHQGCDQRILHFDIKPHNILLDENFNPKISDFGLAKLCSKDQSAVSMTVARGTMGYIAPEVLSRNFGNASYKSDVYSFGMLLLEMVGGRKNIDVTVETNQVYFPEWVYNQLDQGEDVHIRIEEEGDIKIAKKLTIVGLWCIQWYPVDRPSMKVVVQMLEREGDNLTIPPNPFASISSTGGNIRRRKRSLQQELTIISE 598
| Match Name | Stats | Description |
|---|---|---|
| A0A6P6VYH8 | E-Value: 0.000e+0, PID: 50.56 | (rust resistance kinase Lr10-like {ECO:0000313|Ref... [more] |
| A0A5J4ZHM3 | E-Value: 0.000e+0, PID: 52.36 | Protein kinase domain-containing protein {ECO:0000... [more] |
| A0A068VAS2 | E-Value: 0.000e+0, PID: 50.72 | Protein kinase domain-containing protein {ECO:0000... [more] |
| A0A6P6WH70 | E-Value: 0.000e+0, PID: 50.72 | (rust resistance kinase Lr10-like {ECO:0000313|Ref... [more] |
| A0A6P3ZS05 | E-Value: 0.000e+0, PID: 52.02 | (rust resistance kinase Lr10-like isoform X1 {ECO:... [more] |
| A0A6P3ZFU1 | E-Value: 0.000e+0, PID: 52.03 | (rust resistance kinase Lr10-like isoform X2 {ECO:... [more] |
| A0A5J5ARI6 | E-Value: 0.000e+0, PID: 52.12 | Protein kinase domain-containing protein {ECO:0000... [more] |
| A0A5B7AJA7 | E-Value: 0.000e+0, PID: 53.66 | Protein kinase domain-containing protein {ECO:0000... [more] |
| F6H6L7 | E-Value: 0.000e+0, PID: 50.08 | Protein kinase domain-containing protein {ECO:0000... [more] |
| A0A438ICV5 | E-Value: 0.000e+0, PID: 50.16 | (Rust resistance kinase Lr10 {ECO:0000313|EMBL:RVW... [more] |
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back to topAnalysis:
| Name | Description |
|---|---|
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions. | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |