DCAR_016085

Resource Type: 
Polypeptide
Name: 
DCAR_016085
Identifier: 
DCAR_016085.mRNA-protein
Sequence: 
MSHHVSAVITNNTYTNNLNHLLDKFPLSFSATLSTTLKQQQQQQHPLSRS
TKFTRTRPRSHPLLKVSCVDYPRPDIDNTLPFLEAAYLSSFFSTASRPNK
PLNVVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDW
YETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFD
FPEVLPAPLNGNMRWHLSSITWFSLLTTFNYCTGIWAILKNNEMLTWPEK
VKFALGLLPAIIGGQAYVEAQDGLSVQDWMRKQGIPDRVTTEVFVAMSKS
LNFINPDELSMQCVLIALNRFLQEYYDPNKSMLELVFAPAEEWISRSDSD
IIDATMTELARLFPDEIAADQSKAKILKYHVVKTPRLFPDEIAADQSKAK
ILKYHVVKTPRSVYKTIPDCEPCRPLQKSPIEGFYLAGDYTKQRYLASME
GAVLSGKLCAQAILQNGDRACCNGLNEFCMTLVEKLNGVAAVLDGLSGDR
ALCDGLNEFA*
Sequence Length: 
511
Sequence Checksum: 
59db9640bf265414dbfe01b99fb38b09
View location in JBrowse: 
Relationship: 
There is 1 relationship.
Relationships
The polypeptide, DCAR_016085, derives from mRNA, DCAR_016085.
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Blast Results: 
The following BLAST results are available for this feature:
BLAST of DCAR_016085 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
BLAST of DCAR_016085 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: Q2VER7 (Phytoene dehydrogenase {ECO:0000256|RuleBase:RU368016} (1.3.5.5 {ECO:0000256|RuleBase:RU368016}))

HSP 1 Score: 620.542 bits (1599), Expect = 0.000e+0
Identity = 302/334 (90.42%), Postives = 305/334 (91.32%), Query Frame = 0
 
Query:    1 MSHHVSAVITNNTYTNNLNHLLDKFPLSFSATLSTTLKQQQQQQHPLSRSTKFTRTRPRSHPLLKVSCVDYPRPDIDNTLPFLEAAYLSSFFSTASRPNKPLNVVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPEVLPAPLNGNMRWHLSSITWFSLLTTFNYCTGIWAILKNNEMLTWPEKVKFALGLLPAIIGGQAYVEAQDGLSVQDWMRKQGIPDRVTTEVFVAMSKSLNFINPDELSMQCVLIALNRFLQEYYDPNKSMLE 334
            MSHHVSAVITNNTYTNNLNHLLDKFPLSFSATLSTTLKQQQQQQHPLSRSTKFTRTRPRSHPLLKVSCVDYPRPDIDNTLPFLEAAYLSSFFSTASRPNKPLNVVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPEVLPAPLN                       GIWAILKNNEMLTWPEKVKFALGLLPAIIGGQAYVEAQDGLSVQDWMRKQGIPDRVTTEVFVAMSKSLNFINPDELSMQCVLIALNRFLQE +    + L+
Sbjct:    1 MSHHVSAVITNNTYTNNLNHLLDKFPLSFSATLSTTLKQQQQQQHPLSRSTKFTRTRPRSHPLLKVSCVDYPRPDIDNTLPFLEAAYLSSFFSTASRPNKPLNVVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPEVLPAPLN-----------------------GIWAILKNNEMLTWPEKVKFALGLLPAIIGGQAYVEAQDGLSVQDWMRKQGIPDRVTTEVFVAMSKSLNFINPDELSMQCVLIALNRFLQEKHGSKMAFLD 311    

HSP 2 Score: 231.876 bits (590), Expect = 1.088e-66
Identity = 117/146 (80.14%), Postives = 120/146 (82.19%), Query Frame = 0
 
Query:  323 QEYYDPNKSMLELVFAPAEEWISRSDSDIIDATMTELARLFPDEIAADQSKAKILKYHVVKTPRLFPDEIAADQSKAKILKYHVVKTPRSVYKTIPDCEPCRPLQKSPIEGFYLAGDYTKQRYLASMEGAVLSGKLCAQAILQNGD 468
            +EYYDPNKSMLELVFAPAEEWISRSDSDIIDATMTELARLFP                         DEIAADQSKAKILKYHVVKTPRSVYKTIPDCEPCRPLQKSPIEGFYLAGDYTKQRYLASMEGAVLSGKLCAQAILQ+ +
Sbjct:  437 KEYYDPNKSMLELVFAPAEEWISRSDSDIIDATMTELARLFP-------------------------DEIAADQSKAKILKYHVVKTPRSVYKTIPDCEPCRPLQKSPIEGFYLAGDYTKQRYLASMEGAVLSGKLCAQAILQDHE 557    
BLAST of DCAR_016085 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A4S8K464 ((Uncharacterized protein {ECO:0000313|EMBL:THU69593.1}))

HSP 1 Score: 523.087 bits (1346), Expect = 1.044e-180
Identity = 274/453 (60.49%), Postives = 314/453 (69.32%), Query Frame = 0
 
Query:   64 LKVSCVDYPRPDIDNTLPFLEAAYLSSFFSTASRPNKPLNVVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPEVLPAPLNGNMRWHLSSITWFSLLTTFNYCTGIWAILKNNEMLTWPEKVKFALGLLPAIIGGQAYVEAQDGLSVQDWMRKQGIPDRVTTEVFVAMSKSLNFINPDELSMQC-----------------------VLIALNRFLQ-------------------------EYYDPNKSMLELVFAPAEEWISRSDSDIIDATMTELARLFPDEIAADQSKAKILKYHVVKTPRLFPDEIAADQSKAKILKYHVVKTPRSVYKTIPDCEPCRPLQKSPIEGFYLAGDYTKQRYLASMEGAVLSGKLCAQAILQNGD 468
            L+V C D+PRP+++NT+ FLEAA LSS F    RP KPL VVIAGAGLAGLSTAKYLADAGHKPI+LEARDVLGGKVAAWKD+DGDWYETGLHIFFGAYPN+QNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPE LPAP N                       GI+AIL+N+EMLTWPEKV+FALGLLPA++GGQAYVEAQDGL+V +WM++Q +   V  +        + F++ D L +                         V I  +R L+                         EYYDP++SMLELVFAPAE+WIS SD +I+DATM ELA+LFP+EIAADQSKAKILKYHV                         VKTPRSVYKT+PDCEPCRPLQ+SP++GFYLAGDYTKQ+YLASMEGAVLSGKLCAQAI Q+ D
Sbjct:   77 LQVFCKDFPRPELENTVNFLEAAQLSSSFLNGPRPRKPLKVVIAGAGLAGLSTAKYLADAGHKPIVLEARDVLGGKVAAWKDNDGDWYETGLHIFFGAYPNMQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPETLPAPFN-----------------------GIFAILRNSEMLTWPEKVRFALGLLPAMLGGQAYVEAQDGLTVTEWMKRQSLTYIVHQQ--EKHGSKMAFLDVDILKLLLPQEWKDILYFKKLEKLVGVPVINVHIWFDRKLKNTYDHLLFSRSPLLSVYADMSVACKEYYDPDRSMLELVFAPAEQWISCSDQEIVDATMQELAKLFPNEIAADQSKAKILKYHV-------------------------VKTPRSVYKTVPDCEPCRPLQRSPVKGFYLAGDYTKQKYLASMEGAVLSGKLCAQAITQDYD 479    
BLAST of DCAR_016085 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A103YB14 (Phytoene dehydrogenase {ECO:0000256|RuleBase:RU368016} (1.3.5.5 {ECO:0000256|RuleBase:RU368016}))

HSP 1 Score: 467.618 bits (1202), Expect = 6.093e-157
Identity = 238/331 (71.90%), Postives = 262/331 (79.15%), Query Frame = 0
 
Query:    7 AVITNNTYTNNLNHLLDKFPLSFSATLSTTLKQQQQQQHPLSRSTKFTRTRPRSHPLLKVSCVDYPRPDIDNTLPFLEAAYLSSFFSTASRPNKPLNVVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPEVLPAPLNGNMRWHLSSITWFSLL---TTFNYCTGIWAILKNNEMLTWPEKVKFALGLLPAIIGGQAYVEAQDGLSVQDWMRKQGIPDRVTTEVFVAMSKSLNFINPDELSMQCVLIALNRFLQEYYDPNKSMLE 334
            AV  ++    NL H+ D      S  +   L      Q P  RS   TRT+    PL KV CVDYPRP+++NT  FLEAAYLSS F T+ RP KPL +VIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFP+VLPAPLNG     L       LL    T +  +GIWAIL+NNEMLTWPEKVKFA+GLLPA++GGQAYVEAQDGLSV+DWMRKQGIPDRVTTEVF+AMSK+LNFINPDELSMQC+LIALNRFLQE +    + L+
Sbjct:   60 AVTVSSRDGRNLGHM-DVLAFGHSDAMGHRL------QFPGVRSL-ITRTKKNVSPL-KVVCVDYPRPELENTSNFLEAAYLSSTFRTSPRPAKPLEIVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPDVLPAPLNGKKFNDLKQDCQRMLLLKGLTMHTISGIWAILRNNEMLTWPEKVKFAIGLLPAMLGGQAYVEAQDGLSVKDWMRKQGIPDRVTTEVFIAMSKALNFINPDELSMQCILIALNRFLQEKHGSKMAFLD 381    

HSP 2 Score: 222.246 bits (565), Expect = 2.028e-62
Identity = 109/152 (71.71%), Postives = 120/152 (78.95%), Query Frame = 0
 
Query:  323 QEYYDPNKSMLELVFAPAEEWISRSDSDIIDATMTELARLFPDEIAADQSKAKILKYHVVKTPRLFPDEIAADQSKAKILKYHVVKTPRSVYKTIPDCEPCRPLQKSPIEGFYLAGDYTKQRYLASMEGAVLSGKLCAQAILQNGDRACCNG 474
            +EYYDPN+SMLELVFAPAEEWISRSDSDIIDATM+EL+RLFP                         DEIAADQSKAKILKYHVVKTPRSVYKT+PDCEPCRPLQ+SPI+GFYLAGDYTKQ+YLASMEGAVLSGK CAQAI+Q+ +     G
Sbjct:  507 KEYYDPNRSMLELVFAPAEEWISRSDSDIIDATMSELSRLFP-------------------------DEIAADQSKAKILKYHVVKTPRSVYKTVPDCEPCRPLQRSPIQGFYLAGDYTKQKYLASMEGAVLSGKFCAQAIVQDCELLAARG 633    
BLAST of DCAR_016085 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6H2AC76 (Phytoene dehydrogenase {ECO:0000256|RuleBase:RU368016} (1.3.5.5 {ECO:0000256|RuleBase:RU368016}))

HSP 1 Score: 462.996 bits (1190), Expect = 8.319e-156
Identity = 234/333 (70.27%), Postives = 262/333 (78.68%), Query Frame = 0
 
Query:    2 SHHVSAVITNNTYTNNLNHLLDKFPLSFSATLSTTLKQQQQQQHPLSRSTKFTRTRPRSHPLLKVSCVDYPRPDIDNTLPFLEAAYLSSFFSTASRPNKPLNVVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPEVLPAPLNGNMRWHLSSITWFSLLTTFNYCTGIWAILKNNEMLTWPEKVKFALGLLPAIIGGQAYVEAQDGLSVQDWMRKQGIPDRVTTEVFVAMSKSLNFINPDELSMQCVLIALNRFLQEYYDPNKSMLE 334
            S H+++  + N  ++   H +D   L+F  + +      Q+ Q P  RS   TRT+    PL KV C+DYPRP++DNT  FLEAAYLSS F T+ RP KPL +VIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFP+VLPAPLN                       GIWAILKNNEMLTWPEKVKFA+GLLPA++GGQAYVEAQDGLSV+DWMRKQGIPDRVTTEVF+AMSK+LNFINPDELSMQC+LIALNRFLQE +    + L+
Sbjct:   23 STHINSRDSFNISSSGGGHQIDH-ALAFGRSDAMG----QRLQFPSLRSF-ITRTKKNVSPL-KVVCIDYPRPELDNTSNFLEAAYLSSTFRTSPRPAKPLKIVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPDVLPAPLN-----------------------GIWAILKNNEMLTWPEKVKFAIGLLPAMLGGQAYVEAQDGLSVKDWMRKQGIPDRVTTEVFIAMSKALNFINPDELSMQCILIALNRFLQEKHGSKMAFLD 325    

HSP 2 Score: 218.394 bits (555), Expect = 1.648e-61
Identity = 108/152 (71.05%), Postives = 118/152 (77.63%), Query Frame = 0
 
Query:  323 QEYYDPNKSMLELVFAPAEEWISRSDSDIIDATMTELARLFPDEIAADQSKAKILKYHVVKTPRLFPDEIAADQSKAKILKYHVVKTPRSVYKTIPDCEPCRPLQKSPIEGFYLAGDYTKQRYLASMEGAVLSGKLCAQAILQNGDRACCNG 474
            +EYYDPN+SMLELVFAPAEEWIS SDSDIIDATM+EL+RLFP                         DEIAADQSKAKILKYHVVKTPRSVYKT+PDCEPCRPLQ+SP EGFYLAGDYTKQ+YLASMEGAVLSGK CAQAI+Q+ +     G
Sbjct:  451 KEYYDPNRSMLELVFAPAEEWISCSDSDIIDATMSELSRLFP-------------------------DEIAADQSKAKILKYHVVKTPRSVYKTVPDCEPCRPLQRSPFEGFYLAGDYTKQKYLASMEGAVLSGKFCAQAIVQDYELLAARG 577    
BLAST of DCAR_016085 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: Q2HXJ7 (Phytoene dehydrogenase {ECO:0000256|RuleBase:RU368016} (1.3.5.5 {ECO:0000256|RuleBase:RU368016}))

HSP 1 Score: 461.455 bits (1186), Expect = 1.850e-155
Identity = 224/292 (76.71%), Postives = 244/292 (83.56%), Query Frame = 0
 
Query:   43 QQHPLSRSTKFTRTRPRSHPLLKVSCVDYPRPDIDNTLPFLEAAYLSSFFSTASRPNKPLNVVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPEVLPAPLNGNMRWHLSSITWFSLLTTFNYCTGIWAILKNNEMLTWPEKVKFALGLLPAIIGGQAYVEAQDGLSVQDWMRKQGIPDRVTTEVFVAMSKSLNFINPDELSMQCVLIALNRFLQEYYDPNKSMLE 334
             Q PL+RS   T+ R R+   LKV CVDYPRPD+DNT  F+EAAYLSS F  + RP+KPLN+VIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKD DGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFP+VLPAPLN                       GIWAIL+NNEMLTWPEKVKFA+GLLPA++GGQ YVEAQDGLSVQDWMR+QGIPDRVTTEVF+AMSK+LNFINPDELSMQC+LIALNRFLQE +    + L+
Sbjct:   44 HQFPLARSYSSTK-RGRNVTPLKVVCVDYPRPDLDNTSNFMEAAYLSSIFRASPRPSKPLNIVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDKDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPDVLPAPLN-----------------------GIWAILRNNEMLTWPEKVKFAIGLLPAMLGGQPYVEAQDGLSVQDWMRQQGIPDRVTTEVFIAMSKALNFINPDELSMQCILIALNRFLQEKHGSKIAFLD 311    

HSP 2 Score: 222.246 bits (565), Expect = 4.664e-63
Identity = 110/152 (72.37%), Postives = 119/152 (78.29%), Query Frame = 0
 
Query:  323 QEYYDPNKSMLELVFAPAEEWISRSDSDIIDATMTELARLFPDEIAADQSKAKILKYHVVKTPRLFPDEIAADQSKAKILKYHVVKTPRSVYKTIPDCEPCRPLQKSPIEGFYLAGDYTKQRYLASMEGAVLSGKLCAQAILQNGDRACCNG 474
            +EYYDPN+SMLELVFAPAEEWISRSDSDIIDATM+EL+RLFP                         DEIAADQSKAKILKYHVVKTPRSVYKT+PDCEPCRPLQ+SPIEGFYLAGDYTKQ+YLASMEGAVLS K CAQAI+Q+ D     G
Sbjct:  437 KEYYDPNRSMLELVFAPAEEWISRSDSDIIDATMSELSRLFP-------------------------DEIAADQSKAKILKYHVVKTPRSVYKTVPDCEPCRPLQRSPIEGFYLAGDYTKQKYLASMEGAVLSEKFCAQAIVQDYDLLAARG 563    
BLAST of DCAR_016085 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: V5K782 (Phytoene dehydrogenase {ECO:0000256|RuleBase:RU368016} (1.3.5.5 {ECO:0000256|RuleBase:RU368016}) (Fragment))

HSP 1 Score: 460.685 bits (1184), Expect = 1.878e-155
Identity = 224/292 (76.71%), Postives = 245/292 (83.90%), Query Frame = 0
 
Query:   43 QQHPLSRSTKFTRTRPRSHPLLKVSCVDYPRPDIDNTLPFLEAAYLSSFFSTASRPNKPLNVVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPEVLPAPLNGNMRWHLSSITWFSLLTTFNYCTGIWAILKNNEMLTWPEKVKFALGLLPAIIGGQAYVEAQDGLSVQDWMRKQGIPDRVTTEVFVAMSKSLNFINPDELSMQCVLIALNRFLQEYYDPNKSMLE 334
             Q PL+RS  ++  R R+   LKV CVDYPRPD+DNT  FLEAAYLSS F  + RP+KPLN+VIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKD DGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFP+VLPAPLN                       GIWAIL+NNEMLTWPEKVKFA+GLLPA++GGQAYVEAQDGLSVQDWMR++GIPDRVTTEVF+AMSK+LNFINPDELSMQC+LIALNRFLQE +    + L+
Sbjct:   39 HQFPLARS--YSTKRGRNVTPLKVVCVDYPRPDLDNTSNFLEAAYLSSTFRASPRPSKPLNIVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDKDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPDVLPAPLN-----------------------GIWAILRNNEMLTWPEKVKFAIGLLPAMLGGQAYVEAQDGLSVQDWMRQRGIPDRVTTEVFIAMSKALNFINPDELSMQCILIALNRFLQEKHGSKMAFLD 305    

HSP 2 Score: 223.787 bits (569), Expect = 7.905e-64
Identity = 110/146 (75.34%), Postives = 119/146 (81.51%), Query Frame = 0
 
Query:  323 QEYYDPNKSMLELVFAPAEEWISRSDSDIIDATMTELARLFPDEIAADQSKAKILKYHVVKTPRLFPDEIAADQSKAKILKYHVVKTPRSVYKTIPDCEPCRPLQKSPIEGFYLAGDYTKQRYLASMEGAVLSGKLCAQAILQNGD 468
            +EYYDPN+SMLELVFAPAEEWISRSDSDIIDATM+EL+RLFP                         DEIAADQSKAKILKYHVVKTPRSVYKT+PDCEPCRPLQ+SPIEGFYLAGDYTKQ+YLASMEGAVLSGK CAQAI+Q+ D
Sbjct:  431 KEYYDPNRSMLELVFAPAEEWISRSDSDIIDATMSELSRLFP-------------------------DEIAADQSKAKILKYHVVKTPRSVYKTVPDCEPCRPLQRSPIEGFYLAGDYTKQKYLASMEGAVLSGKFCAQAIVQDYD 551    
BLAST of DCAR_016085 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2D3C328 (Phytoene dehydrogenase {ECO:0000256|RuleBase:RU368016} (1.3.5.5 {ECO:0000256|RuleBase:RU368016}))

HSP 1 Score: 460.685 bits (1184), Expect = 5.395e-155
Identity = 226/291 (77.66%), Postives = 244/291 (83.85%), Query Frame = 0
 
Query:   44 QHPLSRSTKFTRTRPRSHPLLKVSCVDYPRPDIDNTLPFLEAAYLSSFFSTASRPNKPLNVVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPEVLPAPLNGNMRWHLSSITWFSLLTTFNYCTGIWAILKNNEMLTWPEKVKFALGLLPAIIGGQAYVEAQDGLSVQDWMRKQGIPDRVTTEVFVAMSKSLNFINPDELSMQCVLIALNRFLQEYYDPNKSMLE 334
            Q P +RS   TRT+    PL KV C+DYPRP++DNT  FLEAAYLSS F T+ RP KPL +VIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFP+VLPAPLN                       GIWAIL+NNEMLTWPEKVKFA+GLLPA++GGQAYVEAQDGLSVQDWMRKQGIPDRVTTEVF+AMSK+LNFINPDELSMQC+LIALNRFLQE +    + L+
Sbjct:   55 QFPGARSF-ITRTKKNVCPL-KVVCIDYPRPELDNTSNFLEAAYLSSVFRTSPRPAKPLKIVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPDVLPAPLN-----------------------GIWAILRNNEMLTWPEKVKFAIGLLPAMLGGQAYVEAQDGLSVQDWMRKQGIPDRVTTEVFIAMSKALNFINPDELSMQCILIALNRFLQEKHGSKMAFLD 320    

HSP 2 Score: 218.009 bits (554), Expect = 2.037e-61
Identity = 107/152 (70.39%), Postives = 119/152 (78.29%), Query Frame = 0
 
Query:  323 QEYYDPNKSMLELVFAPAEEWISRSDSDIIDATMTELARLFPDEIAADQSKAKILKYHVVKTPRLFPDEIAADQSKAKILKYHVVKTPRSVYKTIPDCEPCRPLQKSPIEGFYLAGDYTKQRYLASMEGAVLSGKLCAQAILQNGDRACCNG 474
            +EYYDPN+SMLELVFAPAEEWISRSDSDIIDATM+EL++LFP                         DEIAAD SKAK+LKYHVVKTPRSVYKT+PDCEPCRPLQ+SPIEGFYLAGDYTKQ+YLASMEGAVLSGK CAQAI+Q+ +     G
Sbjct:  446 KEYYDPNRSMLELVFAPAEEWISRSDSDIIDATMSELSKLFP-------------------------DEIAADGSKAKLLKYHVVKTPRSVYKTVPDCEPCRPLQRSPIEGFYLAGDYTKQKYLASMEGAVLSGKFCAQAIVQDYNLLAARG 572    
BLAST of DCAR_016085 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2Z6N503 (Phytoene dehydrogenase {ECO:0000256|RuleBase:RU368016} (1.3.5.5 {ECO:0000256|RuleBase:RU368016}))

HSP 1 Score: 458.759 bits (1179), Expect = 2.080e-154
Identity = 231/336 (68.75%), Postives = 263/336 (78.27%), Query Frame = 0
 
Query:    1 MSHHVSAVITNNTYTNNLNHLLDKFPLSFSATLSTTLKQQQQQQHPLSRSTKFTRT-RPRSH-PLLKVSCVDYPRPDIDNTLPFLEAAYLSSFFSTASRPNKPLNVVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPEVLPAPLNGNMRWHLSSITWFSLLTTFNYCTGIWAILKNNEMLTWPEKVKFALGLLPAIIGGQAYVEAQDGLSVQDWMRKQGIPDRVTTEVFVAMSKSLNFINPDELSMQCVLIALNRFLQEYYDPNKSMLE 334
            +S   S++ +NNT +           LSFS ++S  L          S ST+FT++ R R+H   LKV C+DYPRP+IDNT+ F+EAAYLSS F    RP KPLNVVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKD+DGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMP+KPGEFSRFDFPEVLP+P+N                       GIWAIL+NNEMLTWPEK+KFA+GLLPAI+GGQAYVEAQDGLSV++WMRKQGIP+RVT EVF+AMSK+LNFINPDELSMQC+LIALNRFLQE +    + L+
Sbjct:   17 ISKFSSSINSNNTIS-----------LSFSGSVSMGLNLAS------SSSTRFTKSNRFRNHGSPLKVVCIDYPRPEIDNTVNFIEAAYLSSTFRANPRPTKPLNVVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDEDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPSKPGEFSRFDFPEVLPSPIN-----------------------GIWAILRNNEMLTWPEKIKFAIGLLPAILGGQAYVEAQDGLSVKEWMRKQGIPERVTDEVFIAMSKALNFINPDELSMQCILIALNRFLQEKHGSKMAFLD 312    

HSP 2 Score: 218.394 bits (555), Expect = 1.352e-61
Identity = 106/152 (69.74%), Postives = 120/152 (78.95%), Query Frame = 0
 
Query:  323 QEYYDPNKSMLELVFAPAEEWISRSDSDIIDATMTELARLFPDEIAADQSKAKILKYHVVKTPRLFPDEIAADQSKAKILKYHVVKTPRSVYKTIPDCEPCRPLQKSPIEGFYLAGDYTKQRYLASMEGAVLSGKLCAQAILQNGDRACCNG 474
            +EYY+PN+SMLELVFAPAEEWISRSD DIIDATM+ELA+LFPD                         EI+ADQSKAKILKYHVVKTPRSVYKT+P+CEPCRP+Q+SPIEGFYLAGDYTKQ+YLASMEGAVLSGKLCAQAI+Q+ +     G
Sbjct:  438 KEYYNPNQSMLELVFAPAEEWISRSDEDIIDATMSELAKLFPD-------------------------EISADQSKAKILKYHVVKTPRSVYKTVPNCEPCRPIQRSPIEGFYLAGDYTKQKYLASMEGAVLSGKLCAQAIVQDSELLSTRG 564    
BLAST of DCAR_016085 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2J6L7G7 (Phytoene dehydrogenase {ECO:0000256|RuleBase:RU368016} (1.3.5.5 {ECO:0000256|RuleBase:RU368016}))

HSP 1 Score: 458.759 bits (1179), Expect = 2.575e-154
Identity = 225/291 (77.32%), Postives = 244/291 (83.85%), Query Frame = 0
 
Query:   44 QHPLSRSTKFTRTRPRSHPLLKVSCVDYPRPDIDNTLPFLEAAYLSSFFSTASRPNKPLNVVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPEVLPAPLNGNMRWHLSSITWFSLLTTFNYCTGIWAILKNNEMLTWPEKVKFALGLLPAIIGGQAYVEAQDGLSVQDWMRKQGIPDRVTTEVFVAMSKSLNFINPDELSMQCVLIALNRFLQEYYDPNKSMLE 334
            Q P +RS  FT    ++   LKV CVDYPRPD+DNT  FLEAAYLSS F T+ RP+KPL +VIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFP+VLPAPLN                       GI+AIL+NNEMLTWPEKVKFA+GLLPA++GGQAYVEAQDGLSVQDWMRKQGIPDRVTTEVF+AMSK+LNFINPDELSMQC+LIALNRFLQE +    + L+
Sbjct:   55 QFPSARS--FTPRSKKNVSPLKVVCVDYPRPDLDNTSNFLEAAYLSSTFRTSPRPSKPLKIVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPDVLPAPLN-----------------------GIFAILRNNEMLTWPEKVKFAIGLLPAMLGGQAYVEAQDGLSVQDWMRKQGIPDRVTTEVFIAMSKALNFINPDELSMQCILIALNRFLQEKHGSKMAFLD 320    

HSP 2 Score: 218.779 bits (556), Expect = 1.226e-61
Identity = 108/152 (71.05%), Postives = 119/152 (78.29%), Query Frame = 0
 
Query:  323 QEYYDPNKSMLELVFAPAEEWISRSDSDIIDATMTELARLFPDEIAADQSKAKILKYHVVKTPRLFPDEIAADQSKAKILKYHVVKTPRSVYKTIPDCEPCRPLQKSPIEGFYLAGDYTKQRYLASMEGAVLSGKLCAQAILQNGDRACCNG 474
            +EYYDPNKSMLELVFAPAEEWISRSD+DIIDATM+EL+RLFP                         DEIAADQSKAKILKY VVKTPRSVYKT+PDCEPCRPLQ+SPI+GFYL+GDYTKQ+YLASMEGAVLSGK CAQAILQ+ +     G
Sbjct:  446 KEYYDPNKSMLELVFAPAEEWISRSDTDIIDATMSELSRLFP-------------------------DEIAADQSKAKILKYKVVKTPRSVYKTVPDCEPCRPLQRSPIQGFYLSGDYTKQKYLASMEGAVLSGKFCAQAILQDYEMLATRG 572    
BLAST of DCAR_016085 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2Z4L0R0 (Phytoene dehydrogenase {ECO:0000256|RuleBase:RU368016} (1.3.5.5 {ECO:0000256|RuleBase:RU368016}))

HSP 1 Score: 457.988 bits (1177), Expect = 3.160e-154
Identity = 221/285 (77.54%), Postives = 240/285 (84.21%), Query Frame = 0
 
Query:   50 STKFTRTRPRSHPLLKVSCVDYPRPDIDNTLPFLEAAYLSSFFSTASRPNKPLNVVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNVQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPEVLPAPLNGNMRWHLSSITWFSLLTTFNYCTGIWAILKNNEMLTWPEKVKFALGLLPAIIGGQAYVEAQDGLSVQDWMRKQGIPDRVTTEVFVAMSKSLNFINPDELSMQCVLIALNRFLQEYYDPNKSMLE 334
            +  FT    ++   LKV CVDYPRPD+DNT  FLEAAYLSS F T+ RP+KPL +VIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPN+QNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFP+VLPAPLN                       GIWAIL+NNEMLTWPEKVKFA+GLLPA++GGQAYVEAQDGLSVQDWMRKQGIPDRVTTEVF+AMSK+LNFINPDELSMQC+LIALNRFLQE +    + L+
Sbjct:   35 TRSFTPRSKKNVSPLKVVCVDYPRPDLDNTSNFLEAAYLSSTFRTSPRPSKPLKIVIAGAGLAGLSTAKYLADAGHKPILLEARDVLGGKVAAWKDDDGDWYETGLHIFFGAYPNIQNLFGELGINDRLQWKEHSMIFAMPNKPGEFSRFDFPDVLPAPLN-----------------------GIWAILRNNEMLTWPEKVKFAIGLLPAMLGGQAYVEAQDGLSVQDWMRKQGIPDRVTTEVFIAMSKALNFINPDELSMQCILIALNRFLQEKHGSKMAFLD 296    

HSP 2 Score: 211.075 bits (536), Expect = 5.955e-59
Identity = 104/152 (68.42%), Postives = 117/152 (76.97%), Query Frame = 0
 
Query:  323 QEYYDPNKSMLELVFAPAEEWISRSDSDIIDATMTELARLFPDEIAADQSKAKILKYHVVKTPRLFPDEIAADQSKAKILKYHVVKTPRSVYKTIPDCEPCRPLQKSPIEGFYLAGDYTKQRYLASMEGAVLSGKLCAQAILQNGDRACCNG 474
            +EYYDPN+SMLELVFAPAEEWISRSD+DIIDATM EL+RLFP                         DEI+AD SKAKILKY VVKTPRSVYKT+PDCEPCRPLQ+SPI+GFYLAGDYTKQ+YLASMEGAVLSGK CAQAI+++ +     G
Sbjct:  422 KEYYDPNRSMLELVFAPAEEWISRSDTDIIDATMNELSRLFP-------------------------DEISADGSKAKILKYKVVKTPRSVYKTVPDCEPCRPLQRSPIQGFYLAGDYTKQKYLASMEGAVLSGKFCAQAIVKDYELLASRG 548    
Match NameStatsDescription
Q2VER7E-Value: 1.088e-66, PID: 90.42Phytoene dehydrogenase {ECO:0000256|RuleBase:RU368... [more]
A0A4S8K464E-Value: 1.044e-180, PID: 60.49(Uncharacterized protein {ECO:0000313|EMBL:THU6959... [more]
A0A103YB14E-Value: 6.093e-157, PID: 71.90Phytoene dehydrogenase {ECO:0000256|RuleBase:RU368... [more]
A0A6H2AC76E-Value: 8.319e-156, PID: 70.27Phytoene dehydrogenase {ECO:0000256|RuleBase:RU368... [more]
Q2HXJ7E-Value: 1.850e-155, PID: 76.71Phytoene dehydrogenase {ECO:0000256|RuleBase:RU368... [more]
V5K782E-Value: 1.878e-155, PID: 76.71Phytoene dehydrogenase {ECO:0000256|RuleBase:RU368... [more]
A0A2D3C328E-Value: 5.395e-155, PID: 77.66Phytoene dehydrogenase {ECO:0000256|RuleBase:RU368... [more]
A0A2Z6N503E-Value: 2.080e-154, PID: 68.75Phytoene dehydrogenase {ECO:0000256|RuleBase:RU368... [more]
A0A2J6L7G7E-Value: 2.575e-154, PID: 77.32Phytoene dehydrogenase {ECO:0000256|RuleBase:RU368... [more]
A0A2Z4L0R0E-Value: 3.160e-154, PID: 77.54Phytoene dehydrogenase {ECO:0000256|RuleBase:RU368... [more]

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Analysis: 
NameDescription

An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4.

There are a few different naming schemes for this assembly. First there is the
Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below.
These sequences can be viewed in JBrowse here.

Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0

LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926.

Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence.

RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records.

The NCBI Sequence report lists the correspondences between the various naming methods

Link to the LNRQ01000000.1 master record at NCBI

Raw Reads: Link to SRA accessions used for the genome assembly

This genome is available in the CarrotOmics Blast Search

This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions.

For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins.

Data from this analysis can be viewed in JBrowse here.

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