DCAR_000307

Resource Type: 
Polypeptide
Name: 
DCAR_000307
Identifier: 
DCAR_000307.mRNA-protein
Sequence: 
MPNNVNSPRDSPRASPSPSGSPRIGFTVPAHKPKRPLTSAEIMRQQMQVS
EQSDNRLRKTLMRTLVGQTGRRAETIILPLELLRHLKPSEFKTGGEYHLW
QKRQLKLLEAGLLLHPSIPVEKSNKFAMKLNEIITSSEIKPIDTGKNSEQ
MKTLCNCIAALAWRTSDNESPTDACHWADGYPLNVHIYIALLRTIFDTKD
QTMVLDEVDELVELMKKTWSTLGINKLVHNMCFTWVLFEQYILTREVEID
LLAASLAMMSEIAHDVKTVDRETVVHVKMLKRALSSIKRWCEKKLLDYHA
NFNESRLGVMEILLPLASAVTKISEEVPGYATLVQDDGDIATDLTGNRVD
LYIRSSLRNAFAKMLDDGNMSSDIIEVQDVTDALLQIAQGTHELAAKERE
TYSSILKKWHPVAAGAAAVTLHTCYGTLLKQYLTGTSHLLSNEAIEILHT
AGKLEKFLVQMAVEDSAECSDGGKAIVREMVPYEVDAIITRSLKQWIQER
LKSVKEALQTAKESEVTALSFHFIWH*
Sequence Length: 
527
Sequence Checksum: 
5adf3d9dc328ba58c9099119d92f014c
View location in JBrowse: 
Relationship: 
There is 1 relationship.
Relationships
The polypeptide, DCAR_000307, derives from mRNA, DCAR_000307.
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Blast Results: 
The following BLAST results are available for this feature:
BLAST of DCAR_000307 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
BLAST of DCAR_000307 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5J5C0D0 ((Uncharacterized protein {ECO:0000313|EMBL:KAA8548863.1}))

HSP 1 Score: 711.449 bits (1835), Expect = 0.000e+0
Identity = 341/502 (67.93%), Postives = 414/502 (82.47%), Query Frame = 0
 
Query:   17 SPSGSPRIGFTVPAH--KPKRPLTSAEIMRQQMQVSEQSDNRLRKTLMRTLVGQTGRRAETIILPLELLRHLKPSEFKTGGEYHLWQKRQLKLLEAGLLLHPSIPVEKSNKFAMKLNEIITSSEIKPIDTGKNSEQMKTLCNCIAALAWRTSDNESPTDACHWADGYPLNVHIYIALLRTIFDTKDQTMVLDEVDELVELMKKTWSTLGINKLVHNMCFTWVLFEQYILTREVEIDLLAASLAMMSEIAHDVKTVDRETVVHVKMLKRALSSIKRWCEKKLLDYHANFNESRLGVMEILLPLASAVTKISEE-VPGYATLVQDDGDIATDLTGNRVDLYIRSSLRNAFAKMLDDGNMSSDIIEVQDVTDALLQIAQGTHELAAKERETYSSILKKWHPVAAGAAAVTLHTCYGTLLKQYLTGTSHLLSNEAIEILHTAGKLEKFLVQMAVEDSAECSDGGKAIVREMVPYEVDAIITRSLKQWIQERLKSVKEALQTAKESE 515
            +P  SP I FT+P+   + +RP+TSAEIMRQQM+V+EQSDNRLRKTLMRTLVGQ GRR ETIILPLELLRHLKPSEF +  EYHLWQKRQLK+LEAGL+LHPSIP++KSN FAM+L +II ++E KPIDTGKNSE M+TLCNC+ +L+WR+ D  SPTD CHWADG+PLNVH+Y  LL +IFD +D+T+VLDEVDELVELMKKTWSTLGIN+ +HN+C TWVLFEQY++T +VE +L+ A+LAM++E+A+D K  DRE  ++VKML   L++IKRW EK+LLDYH NFN   +G+ME LLPL  A TKI EE V G A   Q+ G++A D TGNRVD YIRSSLRNAFAK+L++GN++S I+EVQ+VT+ALL++A+ T ELA KE+E +S +LKKWHP+AAG AAVTLH CYGTLLKQYL G S  LS E + IL  A +LEK LVQM VEDS EC DGGK IVREMVPYEVD+II + LK+WIQERLK+ KE LQ AKE+E
Sbjct:  137 NPGSSPGISFTLPSPHVRSRRPMTSAEIMRQQMRVTEQSDNRLRKTLMRTLVGQMGRRVETIILPLELLRHLKPSEFNSSIEYHLWQKRQLKILEAGLILHPSIPLDKSNSFAMRLRDIIRAAETKPIDTGKNSESMRTLCNCVVSLSWRSPDG-SPTDVCHWADGFPLNVHLYTMLLHSIFDLRDETLVLDEVDELVELMKKTWSTLGINRSIHNLCLTWVLFEQYVVTGKVEENLIVATLAMLAEVANDAKRADREP-IYVKMLSSVLTAIKRWTEKRLLDYHGNFNGVTVGLMENLLPLLFAATKILEEDVTGIAVEGQEKGEVAADSTGNRVDQYIRSSLRNAFAKVLENGNVNSMIVEVQEVTEALLKLAEETKELAMKEKEIFSPVLKKWHPIAAGVAAVTLHNCYGTLLKQYLVGVSS-LSRETVPILQKAAQLEKILVQMVVEDSVECEDGGKTIVREMVPYEVDSIICKLLKEWIQERLKTGKECLQRAKETE 635    
BLAST of DCAR_000307 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B7A1W9 ((Uncharacterized protein {ECO:0000313|EMBL:MPA49716.1}) (Fragment))

HSP 1 Score: 706.057 bits (1821), Expect = 0.000e+0
Identity = 340/503 (67.59%), Postives = 413/503 (82.11%), Query Frame = 0
 
Query:   17 SPSGS-PRIGFTVPAH--KPKRPLTSAEIMRQQMQVSEQSDNRLRKTLMRTLVGQTGRRAETIILPLELLRHLKPSEFKTGGEYHLWQKRQLKLLEAGLLLHPSIPVEKSNKFAMKLNEIITSSEIKPIDTGKNSEQMKTLCNCIAALAWRTSDNESPTDACHWADGYPLNVHIYIALLRTIFDTKDQTMVLDEVDELVELMKKTWSTLGINKLVHNMCFTWVLFEQYILTREVEIDLLAASLAMMSEIAHDVKTVDRETVVHVKMLKRALSSIKRWCEKKLLDYHANFNESRLGVMEILLPLASAVTKISEE-VPGYATLVQDDGDIATDLTGNRVDLYIRSSLRNAFAKMLDDGNMSSDIIEVQDVTDALLQIAQGTHELAAKERETYSSILKKWHPVAAGAAAVTLHTCYGTLLKQYLTGTSHLLSNEAIEILHTAGKLEKFLVQMAVEDSAECSDGGKAIVREMVPYEVDAIITRSLKQWIQERLKSVKEALQTAKESE 515
            +P+GS P I FTVP    + +RP+TSAEIMRQQM+V+EQSDNRLRKTLMRTLVGQ GRRAETIILPLELLRHLKPSEF +  EYHLWQKRQLK+LEAGL+LHPSIP++KSN FAM+L +II + E KP DTGKNSE M+TLCNC+ +L+WR+ D  SPTD CHWADG+PLN+HIY  LL +IFD KD+T+VLDEVDELVELMKKTWSTLGIN+ +HN+CFTWVLFEQY++T +VE+DLL A+LAM++E+A+D K  DRE  ++VKML   L++IKRW EK+LLDYH +FN   +G+ME LLPL    TKI EE V G A   ++ G++  D TGNRVD YIRSSLRNAFAK L++G+++  I+EVQ+V++AL+++A+ T ELA KE+E +S +LKKWHP+AAG AAVTLH+CYGTLLKQYL G S  LS+E I IL  AGKLEK LVQM VEDS EC DGGK IVREMVPYEVD+II   LK+W++ERLK  KE LQ AKE+E
Sbjct:  189 NPAGSSPGISFTVPGPQVRSRRPMTSAEIMRQQMRVTEQSDNRLRKTLMRTLVGQMGRRAETIILPLELLRHLKPSEFNSSHEYHLWQKRQLKILEAGLILHPSIPLDKSNTFAMRLRDIIRACETKPFDTGKNSESMRTLCNCVVSLSWRSPDG-SPTDVCHWADGFPLNLHIYTVLLHSIFDLKDETLVLDEVDELVELMKKTWSTLGINRSIHNLCFTWVLFEQYVVTGKVEVDLLCATLAMLNEVANDAKRADREP-IYVKMLLSVLTAIKRWIEKRLLDYHGSFNRVTVGLMENLLPLVFEATKILEEDVTGIAAPGEEKGEVTADSTGNRVDHYIRSSLRNAFAKTLENGSVNGMIVEVQEVSEALIKLAEETKELALKEKEIFSPVLKKWHPIAAGVAAVTLHSCYGTLLKQYLAGASS-LSSETISILQRAGKLEKVLVQMVVEDSVECEDGGKTIVREMVPYEVDSIILNLLKEWVEERLKKGKECLQRAKETE 688    
BLAST of DCAR_000307 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5J5ACH1 ((Uncharacterized protein {ECO:0000313|EMBL:KAA8527798.1}))

HSP 1 Score: 695.271 bits (1793), Expect = 0.000e+0
Identity = 341/515 (66.21%), Postives = 411/515 (79.81%), Query Frame = 0
 
Query:    3 NNVNSPRDSPRASPS-PSGSPRIGFTVPAHKPKRPLTSAEIMRQQMQVSEQSDNRLRKTLMRTLVGQTGRRAETIILPLELLRHLKPSEFKTGGEYHLWQKRQLKLLEAGLLLHPSIPVEKSNKFAMKLNEIITSSEIKPIDTGKNSEQMKTLCNCIAALAWRTSDNESPTDACHWADGYPLNVHIYIALLRTIFDTKDQTMVLDEVDELVELMKKTWSTLGINKLVHNMCFTWVLFEQYILTREVEIDLLAASLAMMSEIAHDVKTVDRETVVHVKMLKRALSSIKRWCEKKLLDYHANFNESRLGVMEILLPLASAVTKISEE-VPGYATLVQDDGDIATDLTGNRVDLYIRSSLRNAFAKMLDDGNMSSDIIEVQDVTDALLQIAQGTHELAAKERETYSSILKKWHPVAAGAAAVTLHTCYGTLLKQYLTGTSHLLSNEAIEILHTAGKLEKFLVQMAVEDSAECSDGGKAIVREMVPYEVDAIITRSLKQWIQERLKSVKEALQTAKESE 515
            ++  S   SP A  S P  SP + FTVP  + +RP+TSAEIMRQQM+V+EQSDNRLRKTLMRTL+G   RRAETIILPLELLRHLKPSEF +  EYHLWQKR LK+LE GL+LHPSIP++KSN FAM+L E I + E KPIDTGKNSE M+TLCNC+ +L+WR+ D  SPTD CHWADG+PLNVHIY  LL +IFD KD+T+VLDE+DEL+ELMKKTWSTLGIN+ +HN+CFTWVLFEQY+ T +VE+DLL A+LAMM+E+A+D K  DRE  ++VKML   L+SIKRW EK+LLDYH +FN+  +G+ME LLPL  + TKI EE V G +    + G+   D TGNRVD YIRSSLRNAFAK L++G ++  I+EV++V++ALL++A  T ELA KE+E +S +LKKWHP++AGAAAVTLHTCYGTLLKQYL G+S  LSNE I IL  AGKLEK LVQM VEDS EC DGGK IVREMVPYEVD+II   LKQWIQ+ LK  +E LQ AKE+E
Sbjct:  120 SSCGSNPSSPNAHNSNPGSSPGMAFTVPQVRSRRPMTSAEIMRQQMKVTEQSDNRLRKTLMRTLMG---RRAETIILPLELLRHLKPSEFNSSHEYHLWQKRLLKILETGLILHPSIPLDKSNTFAMRLREFIRACETKPIDTGKNSESMRTLCNCVVSLSWRSPDG-SPTDVCHWADGFPLNVHIYNVLLHSIFDLKDETLVLDEIDELIELMKKTWSTLGINRSMHNLCFTWVLFEQYVSTGQVEVDLLCATLAMMAEVANDAKRADREP-IYVKMLASVLTSIKRWIEKRLLDYHGSFNKVTVGLMENLLPLVFSATKILEEDVTGISAAGVERGEERADSTGNRVDHYIRSSLRNAFAKTLENGQVNGMIVEVKEVSEALLKLADETEELAMKEKEIFSPMLKKWHPISAGAAAVTLHTCYGTLLKQYLAGSSS-LSNETISILQRAGKLEKVLVQMVVEDSVECEDGGKTIVREMVPYEVDSIILNLLKQWIQQMLKKGRECLQRAKETE 628    
BLAST of DCAR_000307 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A022R198 ((Uncharacterized protein {ECO:0000313|EMBL:EYU32575.1}))

HSP 1 Score: 689.108 bits (1777), Expect = 0.000e+0
Identity = 338/499 (67.74%), Postives = 402/499 (80.56%), Query Frame = 0
 
Query:   23 RIGFTVP-AHKPKRPLTSAEIMRQQMQVSEQSDNRLRKTLMRTLVGQTGRRAETIILPLELLRHLKPSEFKTGGEYHLWQKRQLKLLEAGLLLHPSIPVEKSNKFAMKLNEIITSSEIKPIDTGKNSEQMKTLCNCIAALAWRTSDNESPTDACHWADGYPLNVHIYIALLRTIFDTKDQTMVLDEVDELVELMKKTWSTLGINKLVHNMCFTWVLFEQYILTREVEIDLLAASLAMMSEIAHDVKTVDRETVVHVKMLKRALSSIKRWCEKKLLDYHANFNESRLGVMEILLPLASAVTKISEE-VPGYATLVQDDGDIATD--LTGNRVDLYIRSSLRNAFAKMLDDGNM--SSDIIEVQDVTDALLQIAQGTHELAAKERETYSSILKKWHPVAAGAAAVTLHTCYGTLLKQYLTGTSHLLSNEAIEILHTAGKLEKFLVQMAVEDSAECSDGGKAIVREMVPYEVDAIITRSLKQWIQERLKSVKEALQTAKESE 515
            R+GFTVP A + +RPLTSAEIMRQQM+V+EQSDNRLRKTLMRTLVGQ GRRAETIILPLELLRHLKPSEF    EYHLWQKRQLK++EAGLLLHPSIP+EKS+ FA KL +II SSE K IDTGK S  M+TLCNCI +LAWR+ D  + TD CHWADGYPLNVHIY ALL ++FD KD+T+VLDE+DEL+ELMKKTWS LGIN+ +HN+CFTWVLFEQY+LT +VE DLL AS AM++E+A D K VDR+  V++KML   LS+IK+WCEK+LLDYH NFN   +G+ME +LPL  + TKI EE VP Y T  Q+ G+   D   TGNRVD YIRSSLRNAFAKML+D N+  ++   E Q+V++ L++IA+ T EL  KE++ +S +LKKWHP+AAG AAVTLHTCYGTLLKQYLTG S +L NE + +L  AGKLEK LVQ+ VEDS EC DGGK IVREMVPYEV++ I R LKQWIQERL+  KE L  AK++E
Sbjct:  145 RVGFTVPHAGRMRRPLTSAEIMRQQMRVTEQSDNRLRKTLMRTLVGQMGRRAETIILPLELLRHLKPSEFNDPHEYHLWQKRQLKIIEAGLLLHPSIPLEKSDTFAAKLRDIIDSSETKVIDTGKTSPTMRTLCNCIVSLAWRSPDGGT-TDICHWADGYPLNVHIYTALLSSVFDLKDETLVLDEIDELIELMKKTWSMLGINRSMHNLCFTWVLFEQYVLTGQVEPDLLGASFAMLTEVASDAKKVDRDP-VYLKMLGCCLSAIKKWCEKRLLDYHGNFNRGNIGIMENMLPLLFSATKILEEDVPCYVTSSQEKGEAVVDEHSTGNRVDHYIRSSLRNAFAKMLEDQNVNGTTKYTEAQEVSEVLIKIARKTEELGIKEKDIFSPVLKKWHPIAAGVAAVTLHTCYGTLLKQYLTGKSSIL-NETVLVLQRAGKLEKALVQLVVEDSVECEDGGKTIVREMVPYEVESFIARLLKQWIQERLQKGKEYLLRAKQTE 640    
BLAST of DCAR_000307 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P6WPK2 ((protein unc-13 homolog isoform X2 {ECO:0000313|RefSeq:XP_027116621.1}))

HSP 1 Score: 687.567 bits (1773), Expect = 0.000e+0
Identity = 334/507 (65.88%), Postives = 405/507 (79.88%), Query Frame = 0
 
Query:   14 ASPSPSG----SPRIGFTVPAHKPKRPLTSAEIMRQQMQVSEQSDNRLRKTLMRTLVGQTGRRAETIILPLELLRHLKPSEFKTGGEYHLWQKRQLKLLEAGLLLHPSIPVEKSNKFAMKLNEIITSSEIKPIDTGKNSEQMKTLCNCIAALAWRTSDNESPTDACHWADGYPLNVHIYIALLRTIFDTKDQTMVLDEVDELVELMKKTWSTLGINKLVHNMCFTWVLFEQYILTREVEIDLLAASLAMMSEIAHDVKTVDRETVVHVKMLKRALSSIKRWCEKKLLDYHANFNESRLGVMEILLPLASAVTKISEE-VPGYATLVQDDGDIATDLTGNRVDLYIRSSLRNAFAKMLDDGNMSSDIIEVQDVTDALLQIAQGTHELAAKERETYSSILKKWHPVAAGAAAVTLHTCYGTLLKQYLTGTSHLLSNEAIEILHTAGKLEKFLVQMAVEDSAECSDGGKAIVREMVPYEVDAIITRSLKQWIQERLKSVKEALQTAKESE 515
            A  SP+G    SPR  F+    +P+RP+TSAEIMRQQM+V+EQSDNRLRKTLMRTLVGQ GRRAETIILPLELLRHLKPSEF    EYH WQKRQLK+ E GLLLHPSIP+EKSN+FA++L EII +SE K IDTGKNSE MK+L NC+ +L+WRT+D  SPTD CHWADGYP+NVHIYIALL +IFD K++T+VLDEVDEL+ELMKKTWSTLGIN+ +HN+CFTWVLFE YI+T +VE DLL ASL M++E+A+D K VDRE  ++VKML  AL+S+KRW E +LLDYH NF++  +G+ME ++PL  +  KI EE VPGY  +  + G++A   +GNRVD Y+RSSLRNAFAK+L D N+   ++E+++ ++ L+++A  T  LAA E+E +S +LKKWHPVAAG AAVTLHTCYGTLLKQYL  TS  L+ E   +LH AGKLEK LVQM VEDS EC DGGKAIVREMVPYEVD+II   +KQWIQE LK  KE L  AKE+E
Sbjct:  126 APSSPNGGAGSSPRSAFSSLPSRPRRPMTSAEIMRQQMRVTEQSDNRLRKTLMRTLVGQMGRRAETIILPLELLRHLKPSEFNDPQEYHFWQKRQLKIFEVGLLLHPSIPLEKSNEFAIRLREIIQASEHKAIDTGKNSETMKSLVNCVVSLSWRTADG-SPTDICHWADGYPINVHIYIALLYSIFDIKEETLVLDEVDELLELMKKTWSTLGINRSIHNLCFTWVLFEHYIMTGQVEGDLLGASLTMLAEVANDAKKVDREP-LYVKMLASALTSLKRWAEMRLLDYHQNFHKGTIGLMESIVPLVFSAAKILEEDVPGYLAVELEKGEMANISSGNRVDYYVRSSLRNAFAKILKDRNIDGAVVEMEEASEVLIKLANETERLAAMEKEIFSPVLKKWHPVAAGVAAVTLHTCYGTLLKQYLVATS-ALTEETTTLLHRAGKLEKVLVQMVVEDSVECEDGGKAIVREMVPYEVDSIILNRMKQWIQEILKKGKELLLRAKETE 629    
BLAST of DCAR_000307 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6I9STB7 ((uncharacterized protein LOC105158480 {ECO:0000313|RefSeq:XP_011073565.1}))

HSP 1 Score: 684.871 bits (1766), Expect = 0.000e+0
Identity = 333/496 (67.14%), Postives = 397/496 (80.04%), Query Frame = 0
 
Query:   23 RIGFTVP-AHKPKRPLTSAEIMRQQMQVSEQSDNRLRKTLMRTLVGQTGRRAETIILPLELLRHLKPSEFKTGGEYHLWQKRQLKLLEAGLLLHPSIPVEKSNKFAMKLNEIITSSEIKPIDTGKNSEQMKTLCNCIAALAWRTSDNESPTDACHWADGYPLNVHIYIALLRTIFDTKDQTMVLDEVDELVELMKKTWSTLGINKLVHNMCFTWVLFEQYILTREVEIDLLAASLAMMSEIAHDVKTVDRETVVHVKMLKRALSSIKRWCEKKLLDYHANFNESRLGVMEILLPLASAVTKISEE-VPGYATLVQDDGDIATDLTGNRVDLYIRSSLRNAFAKMLDDGNMS-SDIIEVQDVTDALLQIAQGTHELAAKERETYSSILKKWHPVAAGAAAVTLHTCYGTLLKQYLTGTSHLLSNEAIEILHTAGKLEKFLVQMAVEDSAECSDGGKAIVREMVPYEVDAIITRSLKQWIQERLKSVKEALQTAKESE 515
            R+GFTVP   K +RPLTSAEIMRQQM V+EQSDNRLRKTLMRTLVGQ GRRAETIILPLELLRHLKPSEF    EYHLWQKRQ ++LEAGLLLHPSIP+EKS+  A K  EII SSE+KPIDTGKNS  MKTLCNC+ ALAWR+ D  + TD CHWADG PLNV IY ALL +IFD+KD+T+VLDE+DEL+ELMKKTWSTLGIN+ +HN+CFTWVLFEQY++T+EVE DLL AS  M++E+A D K  DRE  +++KML   L+ +K+WC+K+LLDYH NFN   +G+ME +LPL  + TKI EE VP Y    Q+ GD   + TGNRVDLY+RSSLRNAFA+ML+D N++ +   E Q+V++ L+++A+ T EL  KER+ +S ILKKWHPVAAG AAVTLHTCYGTLLKQYLT  S L  NE + +L  AGKLEK LVQ+ VEDS EC DGGK IVREMVPYEVDA+I R +KQWIQERL+  KE +  AK++E
Sbjct:  151 RVGFTVPQGGKLRRPLTSAEIMRQQMNVTEQSDNRLRKTLMRTLVGQMGRRAETIILPLELLRHLKPSEFNDPHEYHLWQKRQFRILEAGLLLHPSIPLEKSDASAAKFREIIESSEMKPIDTGKNSAVMKTLCNCVVALAWRSPDGGT-TDVCHWADGNPLNVLIYTALLSSIFDSKDETLVLDEIDELLELMKKTWSTLGINRPIHNLCFTWVLFEQYVMTQEVEPDLLGASFVMLTEVATDAKKPDREP-IYMKMLASVLNVMKKWCDKRLLDYHRNFNRETIGLMENMLPLLFSATKILEEDVPYYVNAPQEKGDAGEEPTGNRVDLYVRSSLRNAFAQMLEDLNINGTKTTEPQEVSEVLIKVARKTEELGVKERDIFSPILKKWHPVAAGVAAVTLHTCYGTLLKQYLTSKSSL--NETVLVLQRAGKLEKALVQLVVEDSVECEDGGKMIVREMVPYEVDALIVRLMKQWIQERLQKGKEYILRAKQTE 642    
BLAST of DCAR_000307 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A068TVB0 ((Uncharacterized protein {ECO:0000313|EMBL:CDO99278.1}))

HSP 1 Score: 683.33 bits (1762), Expect = 0.000e+0
Identity = 334/508 (65.75%), Postives = 405/508 (79.72%), Query Frame = 0
 
Query:   14 ASPSPSG----SPRIGFTVPAHKPKRPLTSAEIMRQQMQVSEQSDNRLRKTLMRTLVGQT-GRRAETIILPLELLRHLKPSEFKTGGEYHLWQKRQLKLLEAGLLLHPSIPVEKSNKFAMKLNEIITSSEIKPIDTGKNSEQMKTLCNCIAALAWRTSDNESPTDACHWADGYPLNVHIYIALLRTIFDTKDQTMVLDEVDELVELMKKTWSTLGINKLVHNMCFTWVLFEQYILTREVEIDLLAASLAMMSEIAHDVKTVDRETVVHVKMLKRALSSIKRWCEKKLLDYHANFNESRLGVMEILLPLASAVTKISEE-VPGYATLVQDDGDIATDLTGNRVDLYIRSSLRNAFAKMLDDGNMSSDIIEVQDVTDALLQIAQGTHELAAKERETYSSILKKWHPVAAGAAAVTLHTCYGTLLKQYLTGTSHLLSNEAIEILHTAGKLEKFLVQMAVEDSAECSDGGKAIVREMVPYEVDAIITRSLKQWIQERLKSVKEALQTAKESE 515
            A  SP+G    SPR  F+    +P+RP+TSAEIMRQQM+V+EQSDNRLRKTLMRTLVGQ  GRRAETIILPLELLRHLKPSEF    EYH WQKRQLK+ E GLLLHPSIP+EKSN+FA++L EII +SE K IDTGKNSE MK+L NC+ +L+WRT+D  SPTD CHWADGYP+NVHIYIALL +IFD K++T+VLDEVDEL+ELMKKTWSTLGIN+ +HN+CFTWVLFE YI+T +VE DLL ASL M++E+A+D K VDRE  ++VKML  AL+S+KRW E +LLDYH NF++  +G+ME ++PL  +  KI EE VPGY  +  + G++A   +GNRVD Y+RSSLRNAFAK+L D N+   ++E+++ ++ L+++A  T  LAA E+E +S +LKKWHPVAAG AAVTLHTCYGTLLKQYL  TS  L+ E   +LH AGKLEK LVQM VEDS EC DGGKAIVREMVPYEVD+II   +KQWIQE LK  KE L  AKE+E
Sbjct:  126 APSSPNGGAGSSPRSAFSSLPSRPRRPMTSAEIMRQQMRVTEQSDNRLRKTLMRTLVGQQMGRRAETIILPLELLRHLKPSEFNDPQEYHFWQKRQLKIFEVGLLLHPSIPLEKSNEFAIRLREIIQASEHKAIDTGKNSETMKSLVNCVVSLSWRTADG-SPTDICHWADGYPINVHIYIALLYSIFDIKEETLVLDEVDELLELMKKTWSTLGINRSIHNLCFTWVLFEHYIMTGQVEGDLLGASLTMLAEVANDAKKVDREP-LYVKMLASALTSLKRWAEMRLLDYHQNFHKGTIGLMESIVPLVFSAAKILEEDVPGYLAVELEKGEMANISSGNRVDYYVRSSLRNAFAKILKDRNIDGAVVEMEEASEVLIKLANETERLAAMEKEIFSPVLKKWHPVAAGVAAVTLHTCYGTLLKQYLVATS-ALTEETTTLLHRAGKLEKVLVQMVVEDSVECEDGGKAIVREMVPYEVDSIILNRMKQWIQEILKKGKELLLRAKETE 630    
BLAST of DCAR_000307 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P6WXD9 ((protein unc-13 homolog isoform X2 {ECO:0000313|RefSeq:XP_027120104.1}))

HSP 1 Score: 682.559 bits (1760), Expect = 0.000e+0
Identity = 331/507 (65.29%), Postives = 404/507 (79.68%), Query Frame = 0
 
Query:   14 ASPSPSG----SPRIGFTVPAHKPKRPLTSAEIMRQQMQVSEQSDNRLRKTLMRTLVGQTGRRAETIILPLELLRHLKPSEFKTGGEYHLWQKRQLKLLEAGLLLHPSIPVEKSNKFAMKLNEIITSSEIKPIDTGKNSEQMKTLCNCIAALAWRTSDNESPTDACHWADGYPLNVHIYIALLRTIFDTKDQTMVLDEVDELVELMKKTWSTLGINKLVHNMCFTWVLFEQYILTREVEIDLLAASLAMMSEIAHDVKTVDRETVVHVKMLKRALSSIKRWCEKKLLDYHANFNESRLGVMEILLPLASAVTKISEE-VPGYATLVQDDGDIATDLTGNRVDLYIRSSLRNAFAKMLDDGNMSSDIIEVQDVTDALLQIAQGTHELAAKERETYSSILKKWHPVAAGAAAVTLHTCYGTLLKQYLTGTSHLLSNEAIEILHTAGKLEKFLVQMAVEDSAECSDGGKAIVREMVPYEVDAIITRSLKQWIQERLKSVKEALQTAKESE 515
            A  SP+G    SPR  F+    +P+RP+TSAEIMRQQM+V+EQSDNRLRKTLMRTLVGQ GRRAETIILPLELLRHLKPSEF    EYH WQKRQLK+ E GLLLHPSIP+EKSN+FA++L EII +SE K IDTGKNSE MK+L NC+ +L+WR++D  SPTD CHWADGYP+NVHIYIALL +IFD K++T+VLDEVDEL+ELMKKTWSTLGIN+ +HN+CFTWVLFE YI+T +VE DLL ASL M++E+A+D K VDRE  ++VKML  AL+S+KRW E++LLDYH NF++  +G+ME ++PL  +  KI EE VPGY  +  +  ++    +GNRVD Y+RSSLRNAFAK+L D N+   ++E+++ ++ L+++A  T  LAA E+E +S +LKKWHPVAAG AAVTLHTCYGTLLKQYL  TS  L+ E   +LH AGKLEK LVQM VEDS EC DGGKAIVREMVPYEVD+II   +KQWIQE LK  KE L  AKE+E
Sbjct:  126 APSSPNGGAGTSPRSAFSSLPSRPRRPMTSAEIMRQQMRVTEQSDNRLRKTLMRTLVGQMGRRAETIILPLELLRHLKPSEFNDPQEYHFWQKRQLKIFEVGLLLHPSIPLEKSNEFAIRLREIIQASEHKAIDTGKNSETMKSLVNCVVSLSWRSADG-SPTDICHWADGYPINVHIYIALLYSIFDIKEETLVLDEVDELLELMKKTWSTLGINRSIHNLCFTWVLFEHYIVTGQVEGDLLGASLTMLAEVANDAKKVDREP-LYVKMLASALTSMKRWAERRLLDYHQNFHKGTIGLMESIVPLVFSAAKILEEDVPGYLAVELETEEMTKISSGNRVDYYVRSSLRNAFAKILKDRNIDGAVVEMEEASEVLIKLANETERLAAMEKEIFSPVLKKWHPVAAGVAAVTLHTCYGTLLKQYLVATS-ALTEETTTLLHRAGKLEKVLVQMVVEDSVECEDGGKAIVREMVPYEVDSIILNRMKQWIQEILKKGKELLLRAKETE 629    
BLAST of DCAR_000307 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P6WMY2 ((protein unc-13 homolog isoform X1 {ECO:0000313|RefSeq:XP_027116620.1}))

HSP 1 Score: 681.789 bits (1758), Expect = 0.000e+0
Identity = 334/514 (64.98%), Postives = 405/514 (78.79%), Query Frame = 0
 
Query:   14 ASPSPSG----SPRIGFTVPAHKPKRPLTSAEIMRQQMQVSEQSDNRLRKTLMRTLVGQ-------TGRRAETIILPLELLRHLKPSEFKTGGEYHLWQKRQLKLLEAGLLLHPSIPVEKSNKFAMKLNEIITSSEIKPIDTGKNSEQMKTLCNCIAALAWRTSDNESPTDACHWADGYPLNVHIYIALLRTIFDTKDQTMVLDEVDELVELMKKTWSTLGINKLVHNMCFTWVLFEQYILTREVEIDLLAASLAMMSEIAHDVKTVDRETVVHVKMLKRALSSIKRWCEKKLLDYHANFNESRLGVMEILLPLASAVTKISEE-VPGYATLVQDDGDIATDLTGNRVDLYIRSSLRNAFAKMLDDGNMSSDIIEVQDVTDALLQIAQGTHELAAKERETYSSILKKWHPVAAGAAAVTLHTCYGTLLKQYLTGTSHLLSNEAIEILHTAGKLEKFLVQMAVEDSAECSDGGKAIVREMVPYEVDAIITRSLKQWIQERLKSVKEALQTAKESE 515
            A  SP+G    SPR  F+    +P+RP+TSAEIMRQQM+V+EQSDNRLRKTLMRTLVGQ        GRRAETIILPLELLRHLKPSEF    EYH WQKRQLK+ E GLLLHPSIP+EKSN+FA++L EII +SE K IDTGKNSE MK+L NC+ +L+WRT+D  SPTD CHWADGYP+NVHIYIALL +IFD K++T+VLDEVDEL+ELMKKTWSTLGIN+ +HN+CFTWVLFE YI+T +VE DLL ASL M++E+A+D K VDRE  ++VKML  AL+S+KRW E +LLDYH NF++  +G+ME ++PL  +  KI EE VPGY  +  + G++A   +GNRVD Y+RSSLRNAFAK+L D N+   ++E+++ ++ L+++A  T  LAA E+E +S +LKKWHPVAAG AAVTLHTCYGTLLKQYL  TS  L+ E   +LH AGKLEK LVQM VEDS EC DGGKAIVREMVPYEVD+II   +KQWIQE LK  KE L  AKE+E
Sbjct:  126 APSSPNGGAGSSPRSAFSSLPSRPRRPMTSAEIMRQQMRVTEQSDNRLRKTLMRTLVGQLIQFFEQMGRRAETIILPLELLRHLKPSEFNDPQEYHFWQKRQLKIFEVGLLLHPSIPLEKSNEFAIRLREIIQASEHKAIDTGKNSETMKSLVNCVVSLSWRTADG-SPTDICHWADGYPINVHIYIALLYSIFDIKEETLVLDEVDELLELMKKTWSTLGINRSIHNLCFTWVLFEHYIMTGQVEGDLLGASLTMLAEVANDAKKVDREP-LYVKMLASALTSLKRWAEMRLLDYHQNFHKGTIGLMESIVPLVFSAAKILEEDVPGYLAVELEKGEMANISSGNRVDYYVRSSLRNAFAKILKDRNIDGAVVEMEEASEVLIKLANETERLAAMEKEIFSPVLKKWHPVAAGVAAVTLHTCYGTLLKQYLVATS-ALTEETTTLLHRAGKLEKVLVQMVVEDSVECEDGGKAIVREMVPYEVDSIILNRMKQWIQEILKKGKELLLRAKETE 636    
BLAST of DCAR_000307 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A830BI28 ((Uncharacterized protein {ECO:0000313|EMBL:GFP85832.1}))

HSP 1 Score: 681.404 bits (1757), Expect = 0.000e+0
Identity = 330/484 (68.18%), Postives = 394/484 (81.40%), Query Frame = 0
 
Query:   34 KRPLTSAEIMRQQMQVSEQSDNRLRKTLMRTLVGQTGRRAETIILPLELLRHLKPSEFKTGGEYHLWQKRQLKLLEAGLLLHPSIPVEKSNKFAMKLNEIITSSEIKPIDTGKNSEQMKTLCNCIAALAWRTSDNESPTDACHWADGYPLNVHIYIALLRTIFDTKDQTMVLDEVDELVELMKKTWSTLGINKLVHNMCFTWVLFEQYILTREVEIDLLAASLAMMSEIAHDVKTVDRETVVHVKMLKRALSSIKRWCEKKLLDYHANFNESRLGVMEILLPLASAVTKISEE-VPGYATLVQDDGDIATDLTGNRVDLYIRSSLRNAFAKMLDDGNMS-SDIIEVQDVTDALLQIAQGTHELAAKERETYSSILKKWHPVAAGAAAVTLHTCYGTLLKQYLTGTSHLLSNEAIEILHTAGKLEKFLVQMAVEDSAECSDGGKAIVREMVPYEVDAIITRSLKQWIQERLKSVKEALQTAKESE 515
            +RP+TSAEIMRQQM+V+EQSDNRLRKTLMRTLVGQ GRRAETIILPLELLRHLKPSEF    EYHLWQKRQLK+LEAGLLLHPSIP +KS+ FA KL +II + +IK IDTGKNS  M+TLCNCI +LAWR S N   TD CHWADGYPLNVHIY  LL ++FD KD+T+VLDE+DEL+ELMKKTWSTLGIN+ +HN+CFTWVLFEQY++T +VE DLL+AS AM+SE+AHD K VDRE  +++KML   L S+K+WCEK+LLDYH +FN + + VME +LPL  + TKI EE VP Y T  Q+ G+   D TGNRVD YIRSSLRNAFAKML+D N++ +   E  +V++ L+++A+ T ELAAKE++ +S ILKKWHPVAAG AAVTLHTCYGTLLKQYLTG S  L NE + +L  AGKLEK LVQ+ VEDS EC DGGKA+VREM PYEVDA+I R LKQW+QERL+  KE L  +K++E
Sbjct:    2 RRPMTSAEIMRQQMRVTEQSDNRLRKTLMRTLVGQMGRRAETIILPLELLRHLKPSEFNDPQEYHLWQKRQLKILEAGLLLHPSIPFDKSDTFAAKLRDIINACDIKAIDTGKNSTTMRTLCNCIVSLAWR-SPNGGTTDVCHWADGYPLNVHIYTTLLSSVFDMKDETLVLDEIDELMELMKKTWSTLGINRSIHNLCFTWVLFEQYVVTGQVEPDLLSASFAMLSEVAHDAKKVDREP-IYLKMLASVLVSLKKWCEKRLLDYHGSFNRATVCVMENMLPLLFSATKILEEDVPCYVTSSQEKGEAGDDSTGNRVDYYIRSSLRNAFAKMLEDQNVNGTKYTEGLEVSELLIKLAKKTEELAAKEKDIFSPILKKWHPVAAGVAAVTLHTCYGTLLKQYLTGKSSFL-NETVLVLQRAGKLEKSLVQLVVEDSVECEDGGKAVVREMAPYEVDAMILRLLKQWLQERLQKGKEYLLRSKQTE 482    
Match NameStatsDescription
A0A5J5C0D0E-Value: 0.000e+0, PID: 67.93(Uncharacterized protein {ECO:0000313|EMBL:KAA8548... [more]
A0A5B7A1W9E-Value: 0.000e+0, PID: 67.59(Uncharacterized protein {ECO:0000313|EMBL:MPA4971... [more]
A0A5J5ACH1E-Value: 0.000e+0, PID: 66.21(Uncharacterized protein {ECO:0000313|EMBL:KAA8527... [more]
A0A022R198E-Value: 0.000e+0, PID: 67.74(Uncharacterized protein {ECO:0000313|EMBL:EYU3257... [more]
A0A6P6WPK2E-Value: 0.000e+0, PID: 65.88(protein unc-13 homolog isoform X2 {ECO:0000313|Re... [more]
A0A6I9STB7E-Value: 0.000e+0, PID: 67.14(uncharacterized protein LOC105158480 {ECO:0000313... [more]
A0A068TVB0E-Value: 0.000e+0, PID: 65.75(Uncharacterized protein {ECO:0000313|EMBL:CDO9927... [more]
A0A6P6WXD9E-Value: 0.000e+0, PID: 65.29(protein unc-13 homolog isoform X2 {ECO:0000313|Re... [more]
A0A6P6WMY2E-Value: 0.000e+0, PID: 64.98(protein unc-13 homolog isoform X1 {ECO:0000313|Re... [more]
A0A830BI28E-Value: 0.000e+0, PID: 68.18(Uncharacterized protein {ECO:0000313|EMBL:GFP8583... [more]

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Analysis: 
NameDescription

An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4.

There are a few different naming schemes for this assembly. First there is the
Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below.
These sequences can be viewed in JBrowse here.

Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0

LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926.

Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence.

RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records.

The NCBI Sequence report lists the correspondences between the various naming methods

Link to the LNRQ01000000.1 master record at NCBI

Raw Reads: Link to SRA accessions used for the genome assembly

This genome is available in the CarrotOmics Blast Search

This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions.

For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins.

Data from this analysis can be viewed in JBrowse here.

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