DCAR_000285

Resource Type: 
Polypeptide
Name: 
DCAR_000285
Identifier: 
DCAR_000285.mRNA-protein
Sequence: 
MGGNGKHRWKISFHSKSSSSSSSSFVSQPPVEFLCPISGSLMFEPVIVSS
GQTFEGTCVQVCRDLSFVPSLPDGTFPDFSVLIPNSALKSSILTWCKTAN
SPPPNPPDYLAIQSIVRSRMKHHSDSESDLLRGVGDKPRVLFSHAATEIN
PRVDHFYSSSSSQDSVIANVTVPATPLLPFATRPSCFSNSDDSSSEFEAS
VSEESQLITQLKSADVYDQEQVLISLRKKTRINEESRVSLCTPNLLIALK
SSISSKYSDVQVNAAATIVNLSLHKPNKVKIVRAGIVPSLIDILKGGLPE
SQEHAAGALFSLSLEDDNKTAIGVLGALPPLLHALRSDSERTRNDSALAL
YHLSLIQTNRVKLVKLGAVSTLLTLLKRGELLGRVLLVMCNLALCAEGKS
TMLDSNAVECLVQILRTELESESTRENCVAVLYSLSSGSLRFKGLAREVR
AAEVLRVVEENGSERGREKAKRMLMMLRERDEEAGEEVDWEGVLEGGLSR
YRVGRKVTGPNSTQF*
Sequence Length: 
516
Sequence Checksum: 
4670d1891c6cfa864c82685cd4d20dd5
View location in JBrowse: 
Relationship: 
There is 1 relationship.
Relationships
The polypeptide, DCAR_000285, derives from mRNA, DCAR_000285.
Loading content
Blast Results: 
The following BLAST results are available for this feature:
BLAST of DCAR_000285 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
BLAST of DCAR_000285 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B7B9P1 (RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483} (2.3.2.27 {ECO:0000256|ARBA:ARBA00012483}))

HSP 1 Score: 662.529 bits (1708), Expect = 0.000e+0
Identity = 347/540 (64.26%), Postives = 416/540 (77.04%), Query Frame = 0
 
Query:    1 MGGNGKHRWKISFHSKSSSSSSSSFVSQPPVEFLCPISGSLMFEPVIVSSGQTFEGTCVQVCRDLSFVPSLPDGTFPDFSVLIPNSALKSSILTWCKTANSPPPNPPDYLAIQSIVR-----SRMKHHSD--------SESDLLRGVGDKPRVLFSHAATEINPRVDHFYSSSSSQDSVIANVTVPATPLLPFATRPSCFSN--------SDDSSSEFEASVSEESQLITQLKSADVYDQEQVLISLRKKTRINEESRVSLCTPNLLIALKSSISSKYSDVQVNAAATIVNLSLHKPNKVKIVRAGIVPSLIDILKGGLPESQEHAAGALFSLSLEDDNKTAIGVLGALPPLLHALRSDSERTRNDSALALYHLSLIQTNRVKLVKLGAVSTLLTLLKRGELLGRVLLVMCNLALCAEGKSTMLDSNAVECLVQILR--TELESESTRENCVAVLYSLSSGSLRFKGLAREVRAAEVLRVVEENGSERGREKAKRMLMMLRERDEEAGEEVDWEGVLEGGLS--RYRVGRKVTGPNSTQF 515
            MGGNGKHRWKIS +      SSSS   +PP EFLCPI GSLMF+PV+VSSGQTFE + V+VCR+L F P+L DG+ P+F+  IPN ALKS+IL WC T+ +  P+PPDY ++++ V      SR +   D        SE +LLRGV ++P V+FSHAATE+N R +HFYSSSS ++SVIAN    ATPL PF TRP C+S+        SD++ +    S SE+ + + +LKS +V +QEQ +I+LRK TR  EE+RVSLCTP +L AL+S + S+Y+ VQ NA A +VNLSL K NKVKIVR+G+VP LID+LKGG+ ESQEHAAGALFSLSLEDDNKTAIGVLGAL PLLHALRSDSERTR DSA ALYHLSL+Q+NRVKL+KLGAV TLL +++  ++ GRVLLV+CNLA CAEG+STMLD+NAVECLV +LR  +ELESES RENCVA LYSLS GSLRFKGLAR+ RAAEVLR VEE GSER REKAKRML+MLR RDE   EEVDWEG+LEGG S  RYRVGR   G NST+F
Sbjct:    1 MGGNGKHRWKISIYR-----SSSSKFKEPPKEFLCPIFGSLMFDPVVVSSGQTFERSTVEVCRNLGFTPTLADGSKPNFTTEIPNLALKSTILKWCDTSGTEYPHPPDYSSVENTVHKLMESSRGEEREDDGDSMIRVSERELLRGVAERPPVMFSHAATELNHRANHFYSSSS-EESVIANAA--ATPLHPFTTRPLCYSSSSTSSEIASDETLNP--NSTSEDEEFVVKLKSQEVSEQEQGVITLRKITRTKEETRVSLCTPRVLSALRSLLISRYAGVQTNAVAALVNLSLEKVNKVKIVRSGVVPPLIDVLKGGVAESQEHAAGALFSLSLEDDNKTAIGVLGALQPLLHALRSDSERTRQDSAFALYHLSLVQSNRVKLLKLGAVPTLLAMVRSVDVAGRVLLVLCNLAACAEGRSTMLDANAVECLVGMLRRGSELESESARENCVAALYSLSHGSLRFKGLARDARAAEVLREVEERGSERAREKAKRMLVMLRGRDE---EEVDWEGMLEGGASRTRYRVGRNAYGANSTEF 527    
BLAST of DCAR_000285 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A4S4DN80 (RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483} (2.3.2.27 {ECO:0000256|ARBA:ARBA00012483}))

HSP 1 Score: 643.654 bits (1659), Expect = 0.000e+0
Identity = 341/542 (62.92%), Postives = 412/542 (76.01%), Query Frame = 0
 
Query:    1 MGGNGK-HRWKISFH--SKSSSSSSSSFVSQPPVEFLCPISGSLMFEPVIVSSGQTFEGTCVQVCRDLSFVPSLPDGTFPDFSVLIPNSALKSSILTWCKTANSPPPNPPDYLAIQSIVRSRM---KHHSD------SESDLLRGVGDKPRVLFSHAATEINPRVDHFYSSSSSQDSVIANVTVPATPLLPFATRPSCFSNSDDSSSEFEA---------SVSEESQLITQLKSADVYDQEQVLISLRKKTRINEESRVSLCTPNLLIALKSSISSKYSDVQVNAAATIVNLSLHKPNKVKIVRAGIVPSLIDILKGGLPESQEHAAGALFSLSLEDDNKTAIGVLGALPPLLHALRSDSERTRNDSALALYHLSLIQTNRVKLVKLGAVSTLLTLL--KRGELLGRVLLVMCNLALCAEGKSTMLDSNAVECLVQILRTELESESTRENCVAVLYSLSSGSLRFKGLAREVRAAEVLRVVEENGSERGREKAKRMLMMLRERDEEAG--EEVDWEGVLEGGLS--RYRVGRKVTGPNSTQF 515
            MGGNGK H+WKIS +     SSS S     +PP E++CPISGSLMF+PV+VSSGQTFE  CVQVCR L F P LPDG+ PDFS +IPN  LKS+IL WC ++ +  P  PDY ++++ VR+ M   +   D      SE +LL+GV + P VLFSHAATE+N R +HFYSSSS ++SVIANV   +TPLLPF TRP CFS S  +S+  E          S+SE+ + + +LKS DV++QEQ LI+LRK TR+ EE+RVSLCTP LL AL+  + S+Y  VQ NA A +VNLSL K NKVKIVR+GIV  LID+LKGG  ESQEHAAGALFSL++EDDN+TAIGVLGAL PLLH+LRS+SER R+DSALALYHLSL+Q+NRVKLVKLGAVSTLLT++  + G++ GRV+LV+CNLA CAEG+S MLD+NAVE LV +LR E ESESTRENCVA LYSLS GS RFKGLA+  R  EVLR VE+ GS+R REKAK+ML+MLR RDEE G  EEVDWE +LEGG S  RYRVG  + G NST+F
Sbjct:    1 MGGNGKLHKWKISIYRSPSPSSSKSKHKHKEPPTEYVCPISGSLMFDPVVVSSGQTFESMCVQVCRHLGFAPLLPDGSNPDFSTVIPNLNLKSTILNWCDSSRTERPRLPDYSSVETTVRALMVSAREDDDDSKIRVSERELLKGVAENPPVLFSHAATELNHRANHFYSSSS-EESVIANVQ--STPLLPFTTRPMCFSPSSSTSNSSEVAAAETLNPNSISEDEEFVVKLKSLDVFEQEQGLIALRKLTRMKEEARVSLCTPRLLSALRPLLISRYDAVQTNATAALVNLSLEKANKVKIVRSGIVLPLIDVLKGGFAESQEHAAGALFSLAVEDDNRTAIGVLGALQPLLHSLRSESERRRHDSALALYHLSLVQSNRVKLVKLGAVSTLLTMVVTRIGDMAGRVMLVLCNLAACAEGRSAMLDANAVEILVGMLRGESESESTRENCVAALYSLSHGSFRFKGLAKAARMEEVLREVEKRGSDRAREKAKKMLLMLRGRDEEEGAAEEVDWEALLEGGSSRTRYRVGSSMCGANSTEF 539    
BLAST of DCAR_000285 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A7J7HXQ7 (RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483} (2.3.2.27 {ECO:0000256|ARBA:ARBA00012483}))

HSP 1 Score: 643.654 bits (1659), Expect = 0.000e+0
Identity = 341/542 (62.92%), Postives = 412/542 (76.01%), Query Frame = 0
 
Query:    1 MGGNGK-HRWKISFH--SKSSSSSSSSFVSQPPVEFLCPISGSLMFEPVIVSSGQTFEGTCVQVCRDLSFVPSLPDGTFPDFSVLIPNSALKSSILTWCKTANSPPPNPPDYLAIQSIVRSRM---KHHSD------SESDLLRGVGDKPRVLFSHAATEINPRVDHFYSSSSSQDSVIANVTVPATPLLPFATRPSCFSNSDDSSSEFEA---------SVSEESQLITQLKSADVYDQEQVLISLRKKTRINEESRVSLCTPNLLIALKSSISSKYSDVQVNAAATIVNLSLHKPNKVKIVRAGIVPSLIDILKGGLPESQEHAAGALFSLSLEDDNKTAIGVLGALPPLLHALRSDSERTRNDSALALYHLSLIQTNRVKLVKLGAVSTLLTLL--KRGELLGRVLLVMCNLALCAEGKSTMLDSNAVECLVQILRTELESESTRENCVAVLYSLSSGSLRFKGLAREVRAAEVLRVVEENGSERGREKAKRMLMMLRERDEEAG--EEVDWEGVLEGGLS--RYRVGRKVTGPNSTQF 515
            MGGNGK H+WKIS +     SSS S     +PP E++CPISGSLMF+PV+VSSGQTFE  CVQVCR L F P LPDG+ PDFS +IPN  LKS+IL WC ++ +  P  PDY ++++ VR+ M   +   D      SE +LL+GV + P VLFSHAATE+N R +HFYSSSS ++SVIANV   +TPLLPF TRP CFS S  +S+  E          S+SE+ + + +LKS DV++QEQ LI+LRK TR+ EE+RVSLCTP LL AL+  + S+Y  VQ NA A +VNLSL K NKVKIVR+GIV  LID+LKGG  ESQEHAAGALFSL++EDDN+TAIGVLGAL PLLH+LRS+SER R+DSALALYHLSL+Q+NRVKLVKLGAVSTLLT++  + G++ GRV+LV+CNLA CAEG+S MLD+NAVE LV +LR E ESESTRENCVA LYSLS GS RFKGLA+  R  EVLR VE+ GS+R REKAK+ML+MLR RDEE G  EEVDWE +LEGG S  RYRVG  + G NST+F
Sbjct:    1 MGGNGKLHKWKISIYRSPSPSSSKSKHKHKEPPTEYVCPISGSLMFDPVVVSSGQTFESMCVQVCRHLGFAPLLPDGSNPDFSTVIPNLNLKSTILNWCDSSRTERPRLPDYSSVETTVRALMVSAREDDDDSKIRVSERELLKGVAENPPVLFSHAATELNHRANHFYSSSS-EESVIANVQ--STPLLPFTTRPMCFSPSSSTSNSSEVAAAETLNPNSISEDEEFVVKLKSLDVFEQEQGLIALRKLTRMKEEARVSLCTPRLLSALRPLLISRYDAVQTNATAALVNLSLEKANKVKIVRSGIVLPLIDVLKGGFAESQEHAAGALFSLAVEDDNRTAIGVLGALQPLLHSLRSESERRRHDSALALYHLSLVQSNRVKLVKLGAVSTLLTMVVTRIGDMAGRVMLVLCNLAACAEGRSAMLDANAVEILVGMLRGESESESTRENCVAALYSLSHGSFRFKGLAKAARMEEVLREVEKRGSDRAREKAKKMLLMLRGRDEEEGAAEEVDWEALLEGGSSRTRYRVGSSMCGANSTEF 539    
BLAST of DCAR_000285 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6A4L8F7 (RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483} (2.3.2.27 {ECO:0000256|ARBA:ARBA00012483}) (Fragment))

HSP 1 Score: 625.55 bits (1612), Expect = 0.000e+0
Identity = 335/541 (61.92%), Postives = 400/541 (73.94%), Query Frame = 0
 
Query:    1 MGGNGKHRWKISFHSKSSSSSSSSFVSQPPVEFLCPISGSLMFEPVIVSSGQTFEGTCVQVCRDLSFVPSLPDGTFPDFSVLIPNSALKSSILTWCKTANSPPPNPPDYLAIQSIVRSRMK---HHSD--------SESDLLRGVGDKPRVLFSHAATEINPRVDHFYSSSSSQDSVIANVTVPATPLLPFATRPSCFSN------------SDDSS-SEFEASVSEESQLITQLKSADVYDQEQVLISLRKKTRINEESRVSLCTPNLLIALKSSISSKYSDVQVNAAATIVNLSLHKPNKVKIVRAGIVPSLIDILKGGLPESQEHAAGALFSLSLEDDNKTAIGVLGALPPLLHALRSDSERTRNDSALALYHLSLIQTNRVKLVKLGAVSTLLTLLKRGELLGRVLLVMCNLALCAEGKSTMLDSNAVECLVQILRTELESESTRENCVAVLYSLSSGSLRFKGLAREVRAAEVLRVVEENGSERGREKAKRMLMMLRERDEEAGEEVDWEGVL--EGGLSRYRVGRKVTGPNSTQF 515
            MGGNGKHRWKIS    S  S   +   QPP EF+CPISGSLMF+PV+VSSGQTFE T VQVCRDL   P LPDG+ PDF++ IPNSALKS+IL WC  + +P P  PD  +++S VR+ M      +D        SE +LL GV +KP VLFSHAATE+N R  HFYSSSS ++SVIAN  +PATPLLPF TRP CFS+            SD+ + +    S SE+ + +++LKS D+Y+QEQ LISLRK TR  EE+RVSLCTP LL  ++  + S+Y+ VQ NA A IVN+SL K NKVKIVR+GIVP LID +KGG PESQEHAAGA+FSL+LED+NK AIGVLGA+ PLL ALRS+S R+R+DSALALYHLSL+Q+NRVKLVKLGAVSTLLTL+  GEL GR +LV+CNLA C EG+S MLD+NAVE LV +LR    SEST+ENCVA L+SLS GS+RFKGLAR    AEVLR VEE GSER REKA ++LM++R RDE A      EGVL   GG+SRYRVGR   G NST+F
Sbjct:    1 MGGNGKHRWKISIFRSSFPSKLQN--KQPPAEFICPISGSLMFDPVVVSSGQTFERTSVQVCRDLGLSPPLPDGSKPDFTIAIPNSALKSTILKWCDNSGAPRPAAPDRTSVESAVRTIMDSLIEAADDKDSRIRASERELLEGVAEKPDVLFSHAATELNHRATHFYSSSS-EESVIAN-AIPATPLLPFTTRPLCFSSSLSLSSSSSEVVSDEITINPNPNSASEDEEFVSKLKSPDIYEQEQGLISLRKLTRTKEEARVSLCTPRLLAVIRPLLVSRYAAVQTNAVAVIVNVSLDKVNKVKIVRSGIVPPLIDAMKGGFPESQEHAAGAIFSLALEDENKVAIGVLGAMQPLLLALRSESGRSRHDSALALYHLSLVQSNRVKLVKLGAVSTLLTLVS-GELAGRAMLVLCNLAACVEGRSAMLDANAVEVLVGMLRRREVSESTQENCVAALHSLSHGSMRFKGLARGAGLAEVLREVEERGSERAREKAGKILMVMRGRDEAAAAAT--EGVLLEGGGVSRYRVGRNACGVNSTEF 534    
BLAST of DCAR_000285 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: F6GSV2 (RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483} (2.3.2.27 {ECO:0000256|ARBA:ARBA00012483}))

HSP 1 Score: 617.461 bits (1591), Expect = 0.000e+0
Identity = 333/535 (62.24%), Postives = 407/535 (76.07%), Query Frame = 0
 
Query:    1 MGGNGKHRWKISFHSKSSSSSSSSFVSQPPVEFLCPISGSLMFEPVIVSSGQTFEGTCVQVCRDLSFVPSLPDGTFPDFSVLIPNSALKSSILTWCKTANSPPPNPPDYLAIQSIVRSRMKHHSD-----SESDLLRGVGDKPRVLFSHAATEINPRVDHFYSSSSSQDSVIANVTVPATPLLPFATRPSCFSNSDDSSSE-------FEASVSEESQLITQLKSADVYDQEQVLISLRKKTRINEESRVSLCTPNLLIALKSSISSKYSDVQVNAAATIVNLSLHKPNKVKIVRAGIVPSLIDILKGGLPESQEHAAGALFSLSLEDDNKTAIGVLGALPPLLHALRSDSERTRNDSALALYHLSLIQTNRVKLVKLGAVSTLLTLLKRGELLGRVLLVMCNLALCAEGKSTMLDSNAVECLVQILR-TELESESTRENCVAVLYSLSSGSLRFKGLAREVRAAEVLRVVEENGSERGREKAKRMLMMLRERDEEAGEEVDWEGVLE-GGLS--RYRVG----RKVTGPNSTQF 515
            MGGNGKHRWKISF S      SSS   Q P EF+CPISGSLM +PV+V+SGQTFE   VQVCR+L+FVP L DG+ PDF+V+IPN A+KS+IL WC  +    P  P+Y +++++VR+ M    D     SE +LLR V   P V+FSHAATE+N R +HFYSSSS ++SVIA   VPATPL P  TRPSC+S+   SS E        +   SEE +++ +LKS++V++QE+ LI LR  TR  E+ RVSLCTP +L AL+  ++S+Y  VQ NA A++VNLSL KPNK KIVR+GIVP LID+LKGGLPESQEHAAGALFSL++ED+NKTAIGV+GALPPLLH+LRS+SERTR+DSALALYHLSL Q+NRVKLVKLGA+ TLL ++K G+L  R LL++CN+A   +G+S MLD+NAV+CLV +LR  EL+SEST+ENCVAVLY LS GS+RFKGLARE RA EVLR VEE GS R REKAKRML M+R RDEE  EEVDWEGVLE G LS  RYRVG    + + G  ST+F
Sbjct:    1 MGGNGKHRWKISFRS------SSSMPKQSPKEFMCPISGSLMADPVVVASGQTFERISVQVCRNLAFVPVLGDGSRPDFTVVIPNLAMKSAILNWCAASRVDRPTEPEYGSVETLVRAAMGSDGDDRFETSEKELLRAVPGNPPVMFSHAATEVNHRPNHFYSSSS-EESVIA--AVPATPL-PLTTRPSCYSSFSSSSDEALSLAVTLDPISSEEEEILGKLKSSEVHEQEEGLILLRTITRTKEDLRVSLCTPGVLSALRLLLNSRYGVVQTNAVASVVNLSLEKPNKAKIVRSGIVPPLIDLLKGGLPESQEHAAGALFSLAIEDNNKTAIGVMGALPPLLHSLRSESERTRHDSALALYHLSLDQSNRVKLVKLGAIPTLLAMVKSGDLASRALLILCNMAASGDGRSAMLDANAVDCLVGLLRGKELDSESTQENCVAVLYLLSHGSMRFKGLAREARAVEVLREVEERGSGRAREKAKRMLQMMRGRDEE--EEVDWEGVLESGALSQPRYRVGGAGKQHIHGAYSTEF 523    
BLAST of DCAR_000285 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2R6PS64 (RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483} (2.3.2.27 {ECO:0000256|ARBA:ARBA00012483}))

HSP 1 Score: 610.142 bits (1572), Expect = 0.000e+0
Identity = 336/526 (63.88%), Postives = 406/526 (77.19%), Query Frame = 0
 
Query:    1 MGGNGKHRWKISFHSKSSSSSSSSFVSQPPVEFLCPISGSLMFEPVIVSSGQTFEGTCVQVCRDLSFVPSLPDGTFPDFSVLIPNSALKSSILTWCKTANSPPPNPPDYLAIQSIVRSRMKHHSD----SESDLLRGVGDKPRVLFSHAATEINPRVDHFYSSSSSQDSVIANVTVPATPLLPFATRPSCFSNSDDSSSEFEASV-----SEESQLITQLKSADVYDQEQVLISLRKKTRINEESRVSLCTPNLLIALKSSISSKYSDVQVNAAATIVNLSLHKPNKVKIVRAGIVPSLIDILKGGLPESQEHAAGALFSLSLEDDNKTAIGVLGALPPLLHALRSDSERTRNDSALALYHLSLIQTNRVKLVKLGAVSTLLTLLKRGELLGRVLLVMCNLALCAEGKSTMLDSNAVECLVQILRTELESESTRENCVAVLYSLSSGSLRFKGLAREVRAAEVLRVVEENGSERGREKAKRMLMMLRERDEEAGEEVDWEGVLEGGL--SRYRVGRKVTGPNSTQF 515
            MGGNGK+RWKIS +  SSS        +PP EF+CPISGSLM +PV+VSSGQTFE T VQVCR L F P LPDG+ PDF+V+IPNSALKS+IL WC  + +  P  PDY +++ IVR+ +   +     SE +LLRGV +KP VLF HAATE+N RV+HF  S+SS++SVIANV  PATPLLPFATRP C+S    SS   +  +     SE+ + + +LKS+DV++QEQ +I+LRK TR NEE+RVSLCT  LL +L+  + S+Y+ VQ NA A +VNLSL K NKVKIVR+GI P LID+LKGG  ESQEHAAGA+FSL+LEDDNKTA+GVLGAL PLLHALRS+S+RTR+D+A+ALYHLSL+Q+NRVKLVKLGAVSTLL+LL+ GEL GRV+LV+CNLA CAEG+S +LD+NAVE LV +LR    SESTRENCVA L SLS GSLRFKGLAR   AAEVLR VEE GSER REKA++ML++LR RDE A      E VLE GL  SR+RVGR   GPNST+F
Sbjct:    1 MGGNGKNRWKISIYRSSSSKKKLE-KKEPPTEFICPISGSLMSDPVVVSSGQTFERTSVQVCRALGFSPPLPDGSIPDFTVVIPNSALKSTILKWCDASGADRPTAPDYGSVEDIVRTVVGDGAPKIRVSERELLRGVTEKPAVLFFHAATELNHRVNHF-DSASSEESVIANV--PATPLLPFATRPMCYSEESSSSIASDEGINPSFSSEDEEFVVKLKSSDVFEQEQGVIALRKTTRSNEEARVSLCTSRLLSSLRPLLISRYAVVQTNAVAAVVNLSLEKANKVKIVRSGIAPPLIDVLKGGAAESQEHAAGAIFSLALEDDNKTAMGVLGALQPLLHALRSESDRTRHDAAMALYHLSLVQSNRVKLVKLGAVSTLLSLLRGGELAGRVMLVLCNLASCAEGRSALLDANAVEILVGMLRR--GSESTRENCVAALDSLSHGSLRFKGLARAAGAAEVLRDVEERGSERAREKARKMLLLLRGRDEAAAAAT--EEVLEVGLSRSRHRVGRNSFGPNSTEF 518    
BLAST of DCAR_000285 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2R6PUH6 (RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483} (2.3.2.27 {ECO:0000256|ARBA:ARBA00012483}))

HSP 1 Score: 607.06 bits (1564), Expect = 0.000e+0
Identity = 334/529 (63.14%), Postives = 405/529 (76.56%), Query Frame = 0
 
Query:    1 MGGNGKHRWKISFHSKSSSSSSSSFVSQPPVEFLCPISGSLMFEPVIVSSGQTFEGTCVQVCRDLSFVPSLPDGTFPDFSVLIPNSALKSSILTWCKTANSPPPNPPDYLAIQSIVRSRMKHHS----DSESDLLRGVGDKPRVLFSHAATEINPRVDHFYSSSSSQDSVIANVTVPATPLLPFATRPSCFSNSDDSSSEFEASV-----SEESQLITQLKSADVYDQEQVLISLRKKTRINEESRVSLCTPNLLIALKSSISSKYSDVQVNAAATIVNLSLHKPNKVKIVRAGIVPSLIDILKGGLPESQEHAAGALFSLSLEDDNKTAIGVLGALPPLLHALRSDSERTRNDSALALYHLSLIQTNRVKLVKLGAVSTLLTLLKRGELLGRVLLVMCNLALCAEGKSTMLDSNAVECLVQILRTE---LESESTRENCVAVLYSLSSGSLRFKGLAREVRAAEVLRVVEENGSERGREKAKRMLMMLRERDEEAGEEVDWEGVLEGGLS--RYRVGRKVTGPNSTQF 515
            MGGNGKHRWKIS +  SSS        +PP EF+CPISGSLM +PV+VSSGQTFE T VQVCRDL F P LPDG+ PDF+V+IPNSALKS+IL WC  +    P  PDY +++ +VR+ +         SE +LLRGV +KP VLFSHAATE++PRV+HF  S+SS++SVIANV  PATPLLPFATRP C+S    SS   +  +     SE+ + + +LKS+DV++QEQ LI+LRK TR NEE+RVSLCTP LL +L+  + S+Y+ VQ NA A +VNLSL K NKVKIVR+GI P LID+LKGG  ESQEHAAGA+FSL+LEDDN+ A+GVLGAL PLLHALRS+S+RTR+DSA+ALYHLSL+Q+NRVKLVKLGAVSTLL++L+  EL GRV+LV+CNLA C+EG+S MLD+NAVE LV +LR +    ESESTRENCV+ L SLS GSLRFKGLAR    A VLR VEE GSER REKA++ML++LR RDE A      E V EGG S  R+RVGR   GPNST+F
Sbjct:    1 MGGNGKHRWKISIYRSSSSKKLEK--KEPPSEFICPISGSLMSDPVVVSSGQTFERTSVQVCRDLGFSPPLPDGSIPDFTVVIPNSALKSTILKWCDASGVDCPTAPDYFSVEDVVRTFVGDGDLKIRASERELLRGVAEKPAVLFSHAATELSPRVNHF-DSASSEESVIANV--PATPLLPFATRPMCYSAESSSSIASDEGINPNFSSEDEEFVVKLKSSDVFEQEQSLIALRKITRSNEEARVSLCTPQLLSSLQPLLISRYAVVQTNAVAAVVNLSLEKVNKVKIVRSGIAPPLIDVLKGGAAESQEHAAGAIFSLALEDDNRMAMGVLGALQPLLHALRSESDRTRHDSAMALYHLSLVQSNRVKLVKLGAVSTLLSMLRGSELAGRVMLVLCNLAACSEGRSAMLDANAVEILVGMLRRQGSESESESTRENCVSALDSLSHGSLRFKGLARAAGVAAVLREVEERGSERAREKARKMLLLLRGRDEAAAAAE--EAVFEGGPSRARHRVGRNSYGPNSTEF 522    
BLAST of DCAR_000285 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A834G3W4 ((Uncharacterized protein {ECO:0000313|EMBL:KAF7124597.1}))

HSP 1 Score: 604.749 bits (1558), Expect = 0.000e+0
Identity = 315/503 (62.62%), Postives = 379/503 (75.35%), Query Frame = 0
 
Query:    1 MGGNGKHRWKISFHSKSSSSSSSSFVSQPPVEFLCPISGSLMFEPVIVSSGQTFEGTCVQVCRDLSFVPSLPDGTFPDFSVLIPNSALKSSILTWCKTANSPPPNPPDYLAIQSIVRSRMKHHSD-----------SESDLLRGVGDKPRVLFSHAATEINPRVDHFYSSSSSQDSVIANVTVPATPLLPFATRPSCFSNSDDSSSEFEA-----------SVSEESQLITQLKSADVYDQEQVLISLRKKTRINEESRVSLCTPNLLIALKSSISSKYSDVQVNAAATIVNLSLHKPNKVKIVRAGIVPSLIDILKGGLPESQEHAAGALFSLSLEDDNKTAIGVLGALPPLLHALRSDSERTRNDSALALYHLSLIQTNRVKLVKLGAVSTLLTLLKRGELLGRVLLVMCNLALCAEGKSTMLDSNAVECLVQILRTELESESTRENCVAVLYSLSSGSLRFKGLAREVRAAEVLRVVEENGSERGREKAKRMLMMLRERD 481
            MGGNGKHRWKIS    S   +      QPP EF+CPISGSLMF+PV+VSSGQTFE T VQVCRDL   P LPDG+ PDF++ IPNSALKS+IL WC  + +P P  PD  ++++ VR+ M   ++           SE +LL GV +KP VLFSHAATE+NPR +HFYSSSS ++SVIAN  +PATPLLPF+TRP CFS S  SSS               S +E+ + +++LKS D+Y+QEQ LISLRK TR NEE+RVSLCTP LL  ++  + S+Y+ VQ NA A IVN+SL K NKV IVR+GIVP LID +KGG PESQEHA GA+FSL+LED+NK AIGVLGAL PLLHALRS+SER+R+DSALALYHLSL+Q+NRVKLVKLGAVSTLLT++  GEL GR +LV+CNLA C EG+S MLD+NAVE LV +LR    SEST+ENCVA L+SLS GS+RFKGLAR    AEVLR VEE GSER REKA ++LM++R RD
Sbjct:    1 MGGNGKHRWKISIFRSSKLQNK-----QPPAEFICPISGSLMFDPVVVSSGQTFERTSVQVCRDLGLSPPLPDGSKPDFTIAIPNSALKSTILKWCDNSGTPRPAAPDRTSVENAVRTVMDSLNEAADDKDSRIRVSERELLEGVAEKPDVLFSHAATELNPRANHFYSSSS-EESVIAN-AIPATPLLPFSTRPLCFSASLSSSSSEVVSDEITINPNPNSATEDEEFVSKLKSRDIYEQEQGLISLRKLTRTNEEARVSLCTPRLLAVIRPLLVSRYAAVQTNAVAVIVNVSLDKVNKVNIVRSGIVPPLIDAMKGGCPESQEHATGAIFSLALEDENKVAIGVLGALQPLLHALRSESERSRHDSALALYHLSLVQSNRVKLVKLGAVSTLLTMVS-GELAGRAMLVLCNLAACVEGRSAMLDANAVEVLVGMLRRREVSESTQENCVAALHSLSHGSMRFKGLARGAGLAEVLREVEERGSERAREKAGKILMVMRGRD 495    
BLAST of DCAR_000285 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P5YD82 (RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483} (2.3.2.27 {ECO:0000256|ARBA:ARBA00012483}))

HSP 1 Score: 596.66 bits (1537), Expect = 0.000e+0
Identity = 332/546 (60.81%), Postives = 404/546 (73.99%), Query Frame = 0
 
Query:    1 MGGNGKHRWKISFHSKSSSSSSSSFVSQPPVEFLCPISGSLMFEPVIVSSGQTFEGTCVQVCRDLSFVPSLPDGTFPDFSVLIPNSALKSSILTWCKTANSPPPNPPDYLAIQSIVRSRMKHHSDS-----------ESDLLRGVGDKPRVLFSHAATEINPRVDHFY----SSSSSQDSVIANVTVPATPLLPFATRPSCFSNSDDSSSEFEASVSE-------------ESQLITQLKSADVYDQEQVLISLRKKTRINEESRVSLCTPNLLIALKSSISSKYSDVQVNAAATIVNLSLHKPNKVKIVRAGIVPSLIDILKGGLPESQEHAAGALFSLSLEDDNKTAIGVLGALPPLLHALRSDSERTRNDSALALYHLSLIQTNRVKLVKLGAVSTLLTLLKRGELLGRVLLVMCNLALCAEGKSTMLDSNAVECLVQILRT-ELESESTRENCVAVLYSLSSGSLRFKGLAREVRAAEVLRVVEENGSERGREKAKRMLMMLRERDEEAGEEVDWEGVLEGGLS--RYRVGRKVTGPNSTQF 515
            MGGNGKHRWKI F+ +SSSSS     +QPP+EFLCPISGSLMF+PV+V+SGQ+FE T +QVCRDL F P+LPDG+ PDFS +IPN A+K++ILTWC+   +  P PPDY +++ IVRS+++  S S           E +LL+ V + P VLFSHAATE+ PRV++ +    SSSSS +SVI   + P TPL P ATRP+CFSNS  S S  E + SE             E +L  +LKS+D+ +QE  LISLR+ TR  EE+RVSLC+P LL  L+  I S+YS VQ NA A++VNLSL K NKV IVR+G VP LID LK G  E+QEHAAGALFSL+LED++K AIGVLGAL PLLHALRSDSERT++DSALALYHLSLIQ+NRVKLVKLGAVSTLL ++K G+L  RVLL++CNLA C EGKS MLD+NAV  LV +LR  EL SE+TRENCVA L++LS GS+RFKGLA+E RA EVLR VEE GSER +EKAKR+L M+R R+EE  E      +  GGLS  R+RVGR V G NST F
Sbjct:    1 MGGNGKHRWKILFYHRSSSSSPKHPKTQPPLEFLCPISGSLMFDPVVVTSGQSFERTSIQVCRDLRFTPTLPDGSTPDFSTIIPNLAIKTTILTWCRDHQTQDPTPPDYTSVEKIVRSKVQQQSPSRLSSAPDIRVSERELLKAVAENPPVLFSHAATELGPRVNNLFCSTSSSSSSDESVIVTASSPHTPL-PLATRPACFSNSGSSYSSIEITESETLIPNSQSSSSREEQELFLKLKSSDISEQESGLISLRRITRTKEEARVSLCSPRLLSTLRCLIISRYSVVQTNAIASLVNLSLEKSNKVMIVRSGFVPLLIDALKAGSSEAQEHAAGALFSLALEDESKMAIGVLGALQPLLHALRSDSERTQHDSALALYHLSLIQSNRVKLVKLGAVSTLLLMVKSGDLASRVLLILCNLAACTEGKSAMLDANAVAILVGMLRERELVSEATRENCVAALFALSHGSMRFKGLAKEARAVEVLREVEERGSERAKEKAKRILQMMRGREEEDEEIDWEVALESGGLSRTRHRVGRDVFGANSTMF 545    
BLAST of DCAR_000285 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5N6RPG0 (RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483} (2.3.2.27 {ECO:0000256|ARBA:ARBA00012483}))

HSP 1 Score: 594.734 bits (1532), Expect = 0.000e+0
Identity = 321/543 (59.12%), Postives = 400/543 (73.66%), Query Frame = 0
 
Query:    1 MGGNGKHRWKISFHSKSSSSSSSSFVSQPPVEFLCPISGSLMFEPVIVSSGQTFEGTCVQVCRDLSFVPSLPDGTFPDFSVLIPNSALKSSILTWCKTANSPPPNPPDYLAIQSIVRSRMKHHSD--------SESDLLRGVGDKPRVLFSHAATEINPRVDHFYSSSSSQDSVIANVTVPATPLLPFATRPSCFSNSDDSSSEF-----------EASVSEESQLITQLKSADVYDQEQVLISLRKKTRINEESRVSLCTPNLLIALKSSISSKYSDVQVNAAATIVNLSLHKPNKVKIVRAGIVPSLIDILKGGLPESQEHAAGALFSLSLEDDNKTAIGVLGALPPLLHALRSDSERTRNDSALALYHLSLIQTNRVKLVKLGAVSTLLTLLKRGELLGRVLLVMCNLALCAEGKSTMLDSNAVECLVQILRT----ELESESTRENCVAVLYSLSSGSLRFKGLAREVRAAEVLRVVEENGSERGREKAKRMLMMLRER-DEEAGEEVDWEGVLEG----GLSRYRVGRKVTGPNSTQF 515
            MGGNGKHRWKISFH    S+S      QP  EFLCPISGSLM++PV+VSSGQTFE   VQVCRDL + P L DG+ PDF+ +I N A++S+I++WC+++ +  P  PDY +++ +VR+ M    +        SE +LL GV ++P V+FSHAATE+  RV+HFYSSSS +  VIA    PATPL P ATRP C+S+S  SS               +S  EE Q +++LKS +V++QE+ +++LRK TR  E+ RV LCTP LL A++S ISSKY+ VQ NA A ++NLSL K NK++IVR G VP LID L+GG  +SQEHAAGALFSL+LEDDNK AIGVLGALPPL+HALRSDSERTR+DSALALYHLSLIQ+NRVKLVKLGAV TLL + K G    RVLL++CNLA+CAEG+S+MLD+NAVECLV +LRT     ++SE+TRENCVA LY+LS GS+RF+GLA+E RAAEVLR +E+ GSER R KAKR+L M+R R +EE   + DWEGVL      G +RYRVG    GPNST F
Sbjct:    1 MGGNGKHRWKISFHR---SNSKPMEPKQPLKEFLCPISGSLMYDPVVVSSGQTFERVSVQVCRDLGYSPKLEDGSRPDFTAVIQNLAIRSTIISWCESSGTEQPRAPDYSSLERLVRAEMAAEQEKRSQEIRVSERELLNGVAERPPVMFSHAATELGHRVNHFYSSSSEESVVIA--ASPATPL-PLATRPLCYSSSSSSSEIIESETQARTINPSSSTPEEDQFLSKLKSPEVFEQEEAVVALRKLTRTREDLRVPLCTPRLLSAVRSLISSKYASVQTNAIALLLNLSLQKHNKLQIVRLGFVPLLIDALRGGFADSQEHAAGALFSLALEDDNKMAIGVLGALPPLMHALRSDSERTRHDSALALYHLSLIQSNRVKLVKLGAVPTLLAMAKSGNSASRVLLILCNLAVCAEGRSSMLDANAVECLVGMLRTGEEGAVDSEATRENCVAALYALSQGSMRFRGLAKEARAAEVLREIEDRGSERARAKAKRILQMMRGREEEEPAADEDWEGVLNSGRGVGRTRYRVG---YGPNSTTF 534    
Match NameStatsDescription
A0A5B7B9P1E-Value: 0.000e+0, PID: 64.26RING-type E3 ubiquitin transferase {ECO:0000256|AR... [more]
A0A4S4DN80E-Value: 0.000e+0, PID: 62.92RING-type E3 ubiquitin transferase {ECO:0000256|AR... [more]
A0A7J7HXQ7E-Value: 0.000e+0, PID: 62.92RING-type E3 ubiquitin transferase {ECO:0000256|AR... [more]
A0A6A4L8F7E-Value: 0.000e+0, PID: 61.92RING-type E3 ubiquitin transferase {ECO:0000256|AR... [more]
F6GSV2E-Value: 0.000e+0, PID: 62.24RING-type E3 ubiquitin transferase {ECO:0000256|AR... [more]
A0A2R6PS64E-Value: 0.000e+0, PID: 63.88RING-type E3 ubiquitin transferase {ECO:0000256|AR... [more]
A0A2R6PUH6E-Value: 0.000e+0, PID: 63.14RING-type E3 ubiquitin transferase {ECO:0000256|AR... [more]
A0A834G3W4E-Value: 0.000e+0, PID: 62.62(Uncharacterized protein {ECO:0000313|EMBL:KAF7124... [more]
A0A6P5YD82E-Value: 0.000e+0, PID: 60.81RING-type E3 ubiquitin transferase {ECO:0000256|AR... [more]
A0A5N6RPG0E-Value: 0.000e+0, PID: 59.12RING-type E3 ubiquitin transferase {ECO:0000256|AR... [more]

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Analysis: 
NameDescription

An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4.

There are a few different naming schemes for this assembly. First there is the
Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below.
These sequences can be viewed in JBrowse here.

Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0

LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926.

Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence.

RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records.

The NCBI Sequence report lists the correspondences between the various naming methods

Link to the LNRQ01000000.1 master record at NCBI

Raw Reads: Link to SRA accessions used for the genome assembly

This genome is available in the CarrotOmics Blast Search

This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions.

For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins.

Data from this analysis can be viewed in JBrowse here.

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