DCAR_028276

Resource Type: 
Polypeptide
Name: 
DCAR_028276
Identifier: 
DCAR_028276.mRNA-protein
Sequence: 
MESYCIGGRNFTTMAVFSTCPTWRRPRRKRLRRSNVKVSCGRKSEMRCVK
EVSSCVAVVEDEEFADKEDFVKAGGSELLFVQMQRNKAMDTQSKLAHKLP
RIPIRDSVLDLVVIGCGPAGLALAAESAKLGLRVGLIGPDLPFTNNYGVW
EDEFIDLGLEGCIEHVWRDTIVYLDDGDPIMIGRAYGRVSRHLLHEELLK
RCVESGVSYLSSKVEKIIEAGDGHSLVECENNIVIPCRLATVASGAASGK
LLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDYTKQKVPGVE
AEYPTFLYVMPMSPTRIFFEETCLASKDAMPFDLLKKKLMSRLQTMGIRV
AKTYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAP
NYAAVIANILKSSQMNGMINYGRYTENISMQAWKTLWPQERKRQRAFFLF
GLALILQLDIDGIRTFFQTFFRLPTWMWQGFLGSSLSSVDLVLFAFYMFI
IAPHHLRMSLVRHLLSDPTGATMLNQCAKEQQQAEHSKASVPLSSGILAS
*
Sequence Length: 
551
Sequence Checksum: 
8f06a18d2eb7b58393e45091f003fb0e
View location in JBrowse: 
Relationship: 
There is 1 relationship.
Relationships
The polypeptide, DCAR_028276, derives from mRNA, DCAR_028276.
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Blast Results: 
The following BLAST results are available for this feature:
BLAST of DCAR_028276 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
BLAST of DCAR_028276 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: Q2VEX5 ((Putative lycopene epsilon cyclase {ECO:0000313|EMBL:ABB52073.1}))

HSP 1 Score: 1087.79 bits (2812), Expect = 0.000e+0
Identity = 520/525 (99.05%), Postives = 523/525 (99.62%), Query Frame = 0
 
Query:    1 MESYCIGGRNFTTMAVFSTCPTWRRPRRKRLRRSNVKVSCGRKSEMRCVKEVSSCVAVVEDEEFADKEDFVKAGGSELLFVQMQRNKAMDTQSKLAHKLPRIPIRDSVLDLVVIGCGPAGLALAAESAKLGLRVGLIGPDLPFTNNYGVWEDEFIDLGLEGCIEHVWRDTIVYLDDGDPIMIGRAYGRVSRHLLHEELLKRCVESGVSYLSSKVEKIIEAGDGHSLVECENNIVIPCRLATVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDYTKQKVPGVEAEYPTFLYVMPMSPTRIFFEETCLASKDAMPFDLLKKKLMSRLQTMGIRVAKTYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYAAVIANILKSSQMNGMINYGRYTENISMQAWKTLWPQERKRQRAFFLFGLALILQLDIDGIRTFFQTFFRLPTWMWQGFLGSSLSSVDLVLFAFYMFIIAPHHLRMSLVRHLLSDPTGATMLN 525
            MESYCIGGRNFTTMAVFSTCPTWRRPRRKRLRRSNVKVSCGRKSEMRCVKEV SCVAVVEDEEFAD+EDFVKAGGSELLFVQMQRNKAMDTQSKLAHKLPRIPIRDSVLDLVVIGCGPAGLALAAESAKLGLRVGLIGPDLPFTNNYGVWEDEFIDLGLEGCIEHVWRDTIVYLDDGDPIMIGRAYGRVSRHLLHEELLKRCVESGVSYLSSKVEKIIEAGDGHSLVECENNIVIPCRLATVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDYTKQKVPG+EAEYPTFLYVMPMSPTRIFFEETCLASKDAMPFDLLKKKLMSRLQTMGIRVAKTYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYAAVIANILKSSQMNGMINYGRYTENISMQAWKTLWPQERKRQRAFFLFGLALILQLDIDGIRTFFQTFFRLPTWMWQGFLGSSLSSVDLVLFAFYMFIIAPHHLRMSLVRHLLSDPTGATM+ 
Sbjct:    1 MESYCIGGRNFTTMAVFSTCPTWRRPRRKRLRRSNVKVSCGRKSEMRCVKEVISCVAVVEDEEFADEEDFVKAGGSELLFVQMQRNKAMDTQSKLAHKLPRIPIRDSVLDLVVIGCGPAGLALAAESAKLGLRVGLIGPDLPFTNNYGVWEDEFIDLGLEGCIEHVWRDTIVYLDDGDPIMIGRAYGRVSRHLLHEELLKRCVESGVSYLSSKVEKIIEAGDGHSLVECENNIVIPCRLATVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDYTKQKVPGMEAEYPTFLYVMPMSPTRIFFEETCLASKDAMPFDLLKKKLMSRLQTMGIRVAKTYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYAAVIANILKSSQMNGMINYGRYTENISMQAWKTLWPQERKRQRAFFLFGLALILQLDIDGIRTFFQTFFRLPTWMWQGFLGSSLSSVDLVLFAFYMFIIAPHHLRMSLVRHLLSDPTGATMVK 525    
BLAST of DCAR_028276 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5J4ZWV0 ((Uncharacterized protein {ECO:0000313|EMBL:KAA8523275.1}))

HSP 1 Score: 874.389 bits (2258), Expect = 0.000e+0
Identity = 425/525 (80.95%), Postives = 463/525 (88.19%), Query Frame = 0
 
Query:    5 CIGGRNFTTMAVFSTCPTWRRPRRKRLR----RSNVKVSCGRKS-EMRCVKEVSSCVAVVEDEEFADKEDFVKAGGSELLFVQMQRNKAMDTQSKLAHKLPRIPIRDSVLDLVVIGCGPAGLALAAESAKLGLRVGLIGPDLPFTNNYGVWEDEFIDLGLEGCIEHVWRDTIVYLDDGDPIMIGRAYGRVSRHLLHEELLKRCVESGVSYLSSKVEKIIEAGDGHSLVECENNIVIPCRLATVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDYTKQKVPGVEAEYPTFLYVMPMSPTRIFFEETCLASKDAMPFDLLKKKLMSRLQTMGIRVAKTYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYAAVIANILKSSQMNGMINYGRYTENISMQAWKTLWPQERKRQRAFFLFGLALILQLDIDGIRTFFQTFFRLPTWMWQGFLGSSLSSVDLVLFAFYMFIIAPHHLRMSLVRHLLSDPTGATML 524
            CIG RNF TM V STCPTW   RRKRLR    RS  + S    S ++R      SCVAV ED  FAD+ED++KAGGSELLFVQMQ+ K+MD QSKL  KLP+I I D++LDLVVIGCGPAGLALAAESA+LGL VGLIGPDLPFTNNYGVWEDEF DLGLEGCIEHVW DTIVYLD+GDPI+IGRAYGRVSRHLLHEELL RCVESGVSYLSSKVE+I+EA +G +L+ECE+NIVIPCRLATVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDY K+ V  +EA+YPTFLY MPMSPTRIFFEETCLASKDAMPFDLLKKKLMSRL+TMG+R+ KTYEEEWSYIPVGGSLPNT QKNLAFGAAASMVHPATGYSVVRSLSEAP YA+VIANILK      M+ +   TENISMQAW TLWPQERKRQRAFFLFGLALILQLDI+GIRTFF+TFFRLP WMWQGFLGS+LSS DLVLFAFYMF++AP ++RMSL+RHLLSDPTGATM+
Sbjct:    3 CIGARNFATMVV-STCPTWT-SRRKRLRNKQSRSMHRYSSHISSLQVRSSAGSESCVAVKED--FADEEDYIKAGGSELLFVQMQQKKSMDKQSKLTEKLPQIAIGDTLLDLVVIGCGPAGLALAAESARLGLNVGLIGPDLPFTNNYGVWEDEFKDLGLEGCIEHVWGDTIVYLDNGDPILIGRAYGRVSRHLLHEELLNRCVESGVSYLSSKVERIVEATNGLNLIECEHNIVIPCRLATVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDYIKRTVQCLEAQYPTFLYAMPMSPTRIFFEETCLASKDAMPFDLLKKKLMSRLETMGVRIIKTYEEEWSYIPVGGSLPNTAQKNLAFGAAASMVHPATGYSVVRSLSEAPKYASVIANILKRGHSKDMLTHITSTENISMQAWNTLWPQERKRQRAFFLFGLALILQLDIEGIRTFFRTFFRLPNWMWQGFLGSTLSSADLVLFAFYMFVVAPSNMRMSLIRHLLSDPTGATMI 523    
BLAST of DCAR_028276 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B6YMW7 ((Uncharacterized protein {ECO:0000313|EMBL:MPA33137.1}) (Fragment))

HSP 1 Score: 868.611 bits (2243), Expect = 0.000e+0
Identity = 425/526 (80.80%), Postives = 457/526 (86.88%), Query Frame = 0
 
Query:    5 CIGGRNFTTMAVFSTCPTWRRPRRKRLRRSNVKVSCGRKS------EMRCVKEVSSCVAVVEDEEFADKEDFVKAGGSELLFVQMQRNKAMDTQSKLAHKLPRIPIRDSVLDLVVIGCGPAGLALAAESAKLGLRVGLIGPDLPFTNNYGVWEDEFIDLGLEGCIEHVWRDTIVYLDDGDPIMIGRAYGRVSRHLLHEELLKRCVESGVSYLSSKVEKIIEAGDGHSLVECENNIVIPCRLATVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDYTKQKVPGVEAEYPTFLYVMPMSPTRIFFEETCLASKDAMPFDLLKKKLMSRLQTMGIRVAKTYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYAAVIANILKSSQMNGMINYGRYTENISMQAWKTLWPQERKRQRAFFLFGLALILQLDIDGIRTFFQTFFRLPTWMWQGFLGSSLSSVDLVLFAFYMFIIAPHHLRMSLVRHLLSDPTGATML 524
            CIG RNF  +AV STCPTWR  RRKRLR   V+ S  R S      ++R      SCVA  E E+FAD+ED++KAGGSELLFVQMQ++K MD QSKLA KLP+I I D++LDLVVIGCGPAGLALAAESAKLGL VGLIGPDLPFTNNYGVWEDEF DLGLEGCIEHVWRDTIVYLDD DPI+IGRAYGRVSRHLLHEELL RCVES VSYLSSKVE+IIEA +G SL+ECE+NIVIPCRLATVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRD  KQKV  + A YPTFLY MPMSPTR+FFEETCLAS DAMPFDLLKKKLMSRL+TMG+RV KTYEEEWSYIPVGGSLPNT QKNLAFGAAASMVHPATGYSVVRSLSEAP YA+VIANILK      M+      ENISMQAW  LWPQERKRQRAFFLFGLALIL+LDI+GIRTFF TFFRLP WMWQGFLGS+LSS DLVLFAFYMF+IAP+++RM LVRHLLSDPTGATM+
Sbjct:   58 CIGARNFAAVAV-STCPTWR-SRRKRLRNKQVR-SINRYSSYISSLQVRSSAGSESCVA--EKEDFADEEDYIKAGGSELLFVQMQQSKNMDKQSKLADKLPKISIGDTILDLVVIGCGPAGLALAAESAKLGLNVGLIGPDLPFTNNYGVWEDEFKDLGLEGCIEHVWRDTIVYLDDDDPILIGRAYGRVSRHLLHEELLNRCVESSVSYLSSKVERIIEATNGQSLIECEHNIVIPCRLATVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDCVKQKVQCLGALYPTFLYAMPMSPTRVFFEETCLASNDAMPFDLLKKKLMSRLETMGVRVIKTYEEEWSYIPVGGSLPNTAQKNLAFGAAASMVHPATGYSVVRSLSEAPKYASVIANILKRGHSKDMLTCSMSIENISMQAWNILWPQERKRQRAFFLFGLALILELDIEGIRTFFHTFFRLPDWMWQGFLGSTLSSADLVLFAFYMFVIAPNNMRMCLVRHLLSDPTGATMI 578    
BLAST of DCAR_028276 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2R6R480 ((Lycopene epsilon cyclase {ECO:0000313|EMBL:PSS20792.1}))

HSP 1 Score: 868.226 bits (2242), Expect = 0.000e+0
Identity = 414/525 (78.86%), Postives = 452/525 (86.10%), Query Frame = 0
 
Query:    5 CIGGRNFTTMAVFSTCPTWRRPRRKRLRRSNVKVS-----CGRKSEMRCVKEVSSCVAVVEDEEFADKEDFVKAGGSELLFVQMQRNKAMDTQSKLAHKLPRIPIRDSVLDLVVIGCGPAGLALAAESAKLGLRVGLIGPDLPFTNNYGVWEDEFIDLGLEGCIEHVWRDTIVYLDDGDPIMIGRAYGRVSRHLLHEELLKRCVESGVSYLSSKVEKIIEAGDGHSLVECENNIVIPCRLATVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDYTKQKVPGVEAEYPTFLYVMPMSPTRIFFEETCLASKDAMPFDLLKKKLMSRLQTMGIRVAKTYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYAAVIANILKSSQMNGMINYGRYTENISMQAWKTLWPQERKRQRAFFLFGLALILQLDIDGIRTFFQTFFRLPTWMWQGFLGSSLSSVDLVLFAFYMFIIAPHHLRMSLVRHLLSDPTGATML 524
            CIG RNF+ MAV S CP WR  R++   + N+ +      C    + RC     +   V   EEFAD+ED+VKAGGSELLFVQMQ+NK MD QSKL  KLP I + +++LDLVVIGCGPAGLALAAESAKLGL VGLIGPDLPFTNNYGVWEDEF DLGL GCIEH+W+DTIVYLDD DPIMIGRAYGRVSRHLLHEELLKRCVESGV YLSSKVE+IIEA +GHSL+ECE NIV+PCRLATVASGAASGKLLQYEVGGPRVSVQTAYG+EVEVENNPYDPSLMVFMDYRDY KQKV  +EA+YPTFLY MPMSPTR+FFEETCLASKDAMPFDLLKKKL+SRL+ MG+R+ K YEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAP YA+VI+NILK       I+  R TENISMQAW TLWPQERKRQRAFFLFGLALILQLDIDGIR FFQTFFRLPTWMWQGFLGS+LSS DL LFAFYMF+IAP+++R  L+RHLLSDPTGA ML
Sbjct:    3 CIGARNFSAMAV-SACPYWRSRRKRSGNKQNLLIHWYNSWCKSSLQARCSSSAGNDTCVAVKEEFADEEDYVKAGGSELLFVQMQQNKHMDKQSKLTDKLPGISVGNTILDLVVIGCGPAGLALAAESAKLGLSVGLIGPDLPFTNNYGVWEDEFRDLGLAGCIEHIWQDTIVYLDDNDPIMIGRAYGRVSRHLLHEELLKRCVESGVLYLSSKVERIIEATNGHSLIECEQNIVVPCRLATVASGAASGKLLQYEVGGPRVSVQTAYGIEVEVENNPYDPSLMVFMDYRDYVKQKVECLEAQYPTFLYAMPMSPTRVFFEETCLASKDAMPFDLLKKKLISRLEMMGVRILKFYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPKYASVISNILKQGHSKEKISQSRSTENISMQAWNTLWPQERKRQRAFFLFGLALILQLDIDGIREFFQTFFRLPTWMWQGFLGSTLSSADLALFAFYMFVIAPNNMRKCLIRHLLSDPTGAIML 526    
BLAST of DCAR_028276 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2C9UAD9 ((Uncharacterized protein {ECO:0000313|EMBL:OAY27099.1}))

HSP 1 Score: 864.759 bits (2233), Expect = 0.000e+0
Identity = 424/528 (80.30%), Postives = 455/528 (86.17%), Query Frame = 0
 
Query:    5 CIGGRNFTTMAVFSTCPTWRRPRRKRLRRSNVKVSCGRKSEMRC----VKEVS----SCVAVVEDEEFADKEDFVKAGGSELLFVQMQRNKAMDTQSKLAHKLPRIPIRDSVLDLVVIGCGPAGLALAAESAKLGLRVGLIGPDLPFTNNYGVWEDEFIDLGLEGCIEHVWRDTIVYLDDGDPIMIGRAYGRVSRHLLHEELLKRCVESGVSYLSSKVEKIIEAGDGHSLVECENNIVIPCRLATVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDYTKQKVPGVEAEYPTFLYVMPMSPTRIFFEETCLASKDAMPFDLLKKKLMSRLQTMGIRVAKTYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYAAVIANILKSSQMNGMINYGRYTENISMQAWKTLWPQERKRQRAFFLFGLALILQLDIDGIRTFFQTFFRLPTWMWQGFLGSSLSSVDLVLFAFYMFIIAPHHLRMSLVRHLLSDPTGATML 524
            CIG RNFT MAVFS      R R  R  R       G  S  RC    V+  S    SCVA+   EEFAD+ED++KAGGSEL++VQMQ+NK M+ QSKLA KLP IPI D VLDLVVIGCGPAGLALAAESAKLGL VGLIGPDLPFTNNYGVWEDEF DLGLEGCIEHVWRDTIVYLDD DPI+IGRAYGRVSRHLLHEELL+RCVESGVSYLSSKVE+IIEA DGHSLV CE +IV+ CRLATVASGAASGKLL YEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDY KQKVP +EAEYPTFLY MPMS TR+FFEETCLASKDAMPFDLLK+KLMSRL+TMGIR+ KTYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYA+ IANILK    N  +++ R   NISMQAW TLWPQERKRQRAFFLFGLALILQLDI+GIRTFF TFFRLP+WMWQGFLGS+LSS DLV+FAFYMF IAP+ +RM LVRHL+SDPTGATM+
Sbjct:    3 CIGARNFTAMAVFSFSAGTSRRRTLRTYRQ------GGLSNKRCHLYRVRASSAGTDSCVAI--KEEFADEEDYIKAGGSELVYVQMQQNKDMEQQSKLADKLPPIPIGDEVLDLVVIGCGPAGLALAAESAKLGLSVGLIGPDLPFTNNYGVWEDEFKDLGLEGCIEHVWRDTIVYLDDDDPILIGRAYGRVSRHLLHEELLRRCVESGVSYLSSKVERIIEASDGHSLVACERDIVVSCRLATVASGAASGKLLLYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDYMKQKVPCLEAEYPTFLYAMPMSSTRVFFEETCLASKDAMPFDLLKRKLMSRLETMGIRILKTYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYASTIANILKQDHPNAKLHHKRSNANISMQAWDTLWPQERKRQRAFFLFGLALILQLDIEGIRTFFHTFFRLPSWMWQGFLGSTLSSADLVVFAFYMFFIAPNDMRMCLVRHLVSDPTGATMI 522    
BLAST of DCAR_028276 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P5ZFW6 ((lycopene epsilon cyclase, chloroplastic isoform X2 {ECO:0000313|RefSeq:XP_022751743.1}))

HSP 1 Score: 861.677 bits (2225), Expect = 0.000e+0
Identity = 418/523 (79.92%), Postives = 452/523 (86.42%), Query Frame = 0
 
Query:    5 CIGGRNFTTMAVFSTCPTWRRPRRKRLRRSNVKVSCGRKSEMRCVKEVS---SCVAVVEDEEFADKEDFVKAGGSELLFVQMQRNKAMDTQSKLAHKLPRIPIRDSVLDLVVIGCGPAGLALAAESAKLGLRVGLIGPDLPFTNNYGVWEDEFIDLGLEGCIEHVWRDTIVYLDDGDPIMIGRAYGRVSRHLLHEELLKRCVESGVSYLSSKVEKIIEAGDGHSLVECENNIVIPCRLATVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDYTKQKVPGVEAEYPTFLYVMPMSPTRIFFEETCLASKDAMPFDLLKKKLMSRLQTMGIRVAKTYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYAAVIANILKSSQMNGMINYGRYTENISMQAWKTLWPQERKRQRAFFLFGLALILQLDIDGIRTFFQTFFRLPTWMWQGFLGSSLSSVDLVLFAFYMFIIAPHHLRMSLVRHLLSDPTGATML 524
            C+G RNF  MAV STC TWR  RR  +R      S  R    +     +   SCVAV ED  FAD+ED++KAGGSELLFVQMQ+NK MD QSKLA KLP I I D+VLDLVVIGCGPAGLALAAESAKLGL VGLIGPDLPFTNNYGVWEDEF DLGLE CIEHVW+DTIVYLDD  PIMIGRAYGRVSRHLLHEELL+RCVESGV YLSSKVE IIE  DGHSLV CE+N V+PCRL TVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDY KQ+ P +EA+YPTFLY MP+S TR+FFEETCLASKDAMPFDLLKKKL+SRL+TMGI+++K YEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYA+VIANILK     GM+N  R   NISMQAW TLWPQERKRQR+FFLFGLALILQLDI+GIRTFF TFFRLP+WMWQGFLGS+LSS DLVLFAFYMF++AP+ +RM LVRHLLSDPTGATM+
Sbjct:    5 CVGSRNFAAMAV-STCATWRSRRRNLMRTKTALRSHHRFISFKVRASGAGSESCVAVKED--FADEEDYIKAGGSELLFVQMQQNKEMDKQSKLADKLPPISIGDNVLDLVVIGCGPAGLALAAESAKLGLNVGLIGPDLPFTNNYGVWEDEFKDLGLERCIEHVWQDTIVYLDDDKPIMIGRAYGRVSRHLLHEELLRRCVESGVVYLSSKVETIIEGTDGHSLVACEDNRVVPCRLVTVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDYAKQEFPCLEAQYPTFLYAMPVSSTRVFFEETCLASKDAMPFDLLKKKLVSRLETMGIQISKVYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYASVIANILKKDHSKGMLNSVRNNGNISMQAWNTLWPQERKRQRSFFLFGLALILQLDIEGIRTFFHTFFRLPSWMWQGFLGSTLSSADLVLFAFYMFVLAPNDMRMCLVRHLLSDPTGATMI 524    
BLAST of DCAR_028276 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A061GLV2 ((Lycopene beta/epsilon cyclase protein {ECO:0000313|EMBL:EOY30388.1}))

HSP 1 Score: 861.292 bits (2224), Expect = 0.000e+0
Identity = 427/532 (80.26%), Postives = 451/532 (84.77%), Query Frame = 0
 
Query:    5 CIGGRNFTTMAVFSTCPTWRRPRRKRLRRSNVKVS------------CGRKSEMRCVKEVSSCVAVVEDEEFADKEDFVKAGGSELLFVQMQRNKAMDTQSKLAHKLPRIPIRDSVLDLVVIGCGPAGLALAAESAKLGLRVGLIGPDLPFTNNYGVWEDEFIDLGLEGCIEHVWRDTIVYLDDGDPIMIGRAYGRVSRHLLHEELLKRCVESGVSYLSSKVEKIIEAGDGHSLVECENNIVIPCRLATVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDYTKQKVPGVEAEYPTFLYVMPMSPTRIFFEETCLASKDAMPFDLLKKKLMSRLQTMGIRVAKTYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYAAVIANILKSSQMNGMINYGRYTENISMQAWKTLWPQERKRQRAFFLFGLALILQLDIDGIRTFFQTFFRLPTWMWQGFLGSSLSSVDLVLFAFYMFIIAPHHLRMSLVRHLLSDPTGATML 524
            C+G R F  MAV STC  WR  RR  L R+   +S             GR SE        SCVAV ED  FAD+EDF+KAGGSEL+FVQMQ+NK MD QSKLA KLP I I D+VLDLVVIGCGPAGLALAAESAKLGL VGLIGPDLPFTNNYGVWEDEF DLGLEGCIEHVWRDTIVYLDD  PIMIGRAYGRVSR LLHEELL+RCVESGV YLSSKVE IIEA DGH+LV CE+N V+PCRL TVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDY KQ+VP  EA+YPTFLY MP+S TR+FFEETCLASKDAMPFDLLKKKLMSRL+TMGI + K YEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYA+VIANILK     GM    R   NISMQAWKTLWPQERKRQRAFFLFGLALILQLDIDGIRTFF TFFRLP+WMWQGFLGS+LSS DLVLFAFYMF+IAP+ LRM LVRHLLSDPTGATM+
Sbjct:    5 CVGARYFAAMAV-STCAAWR-SRRGNLMRTKTVLSKNHGFISFKVRASGRGSE--------SCVAVKED--FADEEDFIKAGGSELVFVQMQQNKEMDKQSKLADKLPPISIGDNVLDLVVIGCGPAGLALAAESAKLGLNVGLIGPDLPFTNNYGVWEDEFKDLGLEGCIEHVWRDTIVYLDDDKPIMIGRAYGRVSRRLLHEELLRRCVESGVMYLSSKVETIIEATDGHNLVACEHNRVVPCRLVTVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDYAKQEVPCSEAQYPTFLYAMPLSSTRVFFEETCLASKDAMPFDLLKKKLMSRLETMGITILKVYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYASVIANILKKDHSKGMRTSERNNGNISMQAWKTLWPQERKRQRAFFLFGLALILQLDIDGIRTFFNTFFRLPSWMWQGFLGSNLSSADLVLFAFYMFVIAPNDLRMCLVRHLLSDPTGATMI 524    
BLAST of DCAR_028276 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A067KCI7 ((Uncharacterized protein {ECO:0000313|EMBL:KDP33847.1}))

HSP 1 Score: 860.522 bits (2222), Expect = 0.000e+0
Identity = 419/524 (79.96%), Postives = 457/524 (87.21%), Query Frame = 0
 
Query:    5 CIGGRNFTTMAVFSTCPTWRRPRRKRLRRSNVKVSCGRKSEMRCVKEVS----SCVAVVEDEEFADKEDFVKAGGSELLFVQMQRNKAMDTQSKLAHKLPRIPIRDSVLDLVVIGCGPAGLALAAESAKLGLRVGLIGPDLPFTNNYGVWEDEFIDLGLEGCIEHVWRDTIVYLDDGDPIMIGRAYGRVSRHLLHEELLKRCVESGVSYLSSKVEKIIEAGDGHSLVECENNIVIPCRLATVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDYTKQKVPGVEAEYPTFLYVMPMSPTRIFFEETCLASKDAMPFDLLKKKLMSRLQTMGIRVAKTYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYAAVIANILKSSQMNGMINYGRYTENISMQAWKTLWPQERKRQRAFFLFGLALILQLDIDGIRTFFQTFFRLPTWMWQGFLGSSLSSVDLVLFAFYMFIIAPHHLRMSLVRHLLSDPTGATML 524
            CIG RNF    VFS  PT R  RR+ LR S       ++S +  V+  S    SCVA+   EE+AD+ED++KAGGSEL+FV+MQ+NK M+ QSKLA KLP IPI D VLDLVVIGCGPAGLALAAESAKLGL VGLIGPDLPFTNNYGVWEDEF  LGL+GCIEHVW+DTIVYLD+ DPI+IGRAYGRVSRHLLHEELL+RCVESGVSYLSSKVE+IIEA +GHSLV CE++IV+PCRLATVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDY KQKVP +EAEYPTFLY MPMSPTR+FFEETCLASKDAMPFDLLKKKLMSRL+TM IR+ KTYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYA+VIANILK      M+ +     NISMQAW  LWPQERKRQRAFFLFGLALILQLDI+GIRTFF TFFRLP WMWQGFLGS+LSS DL+LFAFYMF+IAP+ +RM LVRHLLSDPTGATM+
Sbjct:    3 CIGARNFAATTVFS-FPTGRT-RRRILRTSKHVGISEKRSRLYQVRASSAGSESCVAI--KEEYADEEDYIKAGGSELVFVEMQQNKDMEKQSKLADKLPPIPIGDDVLDLVVIGCGPAGLALAAESAKLGLSVGLIGPDLPFTNNYGVWEDEFKGLGLDGCIEHVWQDTIVYLDNDDPILIGRAYGRVSRHLLHEELLRRCVESGVSYLSSKVERIIEAANGHSLVACEHDIVVPCRLATVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDYIKQKVPCLEAEYPTFLYAMPMSPTRVFFEETCLASKDAMPFDLLKKKLMSRLETMQIRILKTYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYASVIANILKQGHSKSMLTHKISNANISMQAWNALWPQERKRQRAFFLFGLALILQLDIEGIRTFFHTFFRLPNWMWQGFLGSTLSSADLILFAFYMFVIAPNDMRMCLVRHLLSDPTGATMI 522    
BLAST of DCAR_028276 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A7J7G232 ((Uncharacterized protein {ECO:0000313|EMBL:KAF5934783.1}))

HSP 1 Score: 857.055 bits (2213), Expect = 0.000e+0
Identity = 418/527 (79.32%), Postives = 459/527 (87.10%), Query Frame = 0
 
Query:    5 CIGGRNFTTMAVFSTCPTWRRPRRKRLRRSNVKVSCGRKSEMRCVKEVSS------CVAVVEDEEFADKEDFVKAGGSELLFVQMQRNKAMDTQSKLAHKLPRIPIRDSVLDLVVIGCGPAGLALAAESAKLGLRVGLIGPDLPFTNNYGVWEDEFIDLGLEGCIEHVWRDTIVYLDDGDPIMIGRAYGRVSRHLLHEELLKRCVESGVSYLSSKVEKIIEAGDGHSLVECENNIVIPCRLATVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDYTKQKVPGVEAEYPTFLYVMPMSPTRIFFEETCLASKDAMPFDLLKKKLMSRLQTMGIRVAKTYEE-EWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYAAVIANILKSSQMNGMINYGRYTENISMQAWKTLWPQERKRQRAFFLFGLALILQLDIDGIRTFFQTFFRLPTWMWQGFLGSSLSSVDLVLFAFYMFIIAPHHLRMSLVRHLLSDPTGATML 524
            CIG RNF  MAV STCPTWR  R+KR R +  +V     S    ++  SS      CVA    E FAD+ED++KAGGSELLFVQMQ+NK M+ QSKLA KLPRI + +++LDLVVIGCGPAGLALAAESAKLGL VGLIGPDLPFTNNYGVWEDEF DLGLEGCIEHVW DTIVYLDD DPI+IGRAYGRVSR+LLHEEL+KRCVESGVSYLSSKVE+IIE+ +GHSL+ECE N+V+PCRLATVASGAASGKLLQYEVGGPRVSVQTAYG+EVEVENNPYDP+LMVFMDYRDY K KV  +EA+YPTFLY MPMSPTR+FFEETCLASKDAMPFD+LKKKL+SRL+TMG+RV KTYEE EWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAP YA+VIANILK       ++    TENISM AW TLWPQERKRQRAFFLFGLALILQLDIDGIRTFF TFFRLPTWMWQGFLGS+LSS DL LFAFYMF+IAP+++RM LVRHLLSDPTGATM+
Sbjct:    3 CIGARNFAAMAV-STCPTWRY-RKKRQRNTLTRVIHRHSSYTSSLRVRSSSAGSDSCVAF--KEGFADEEDYIKAGGSELLFVQMQQNKLMEKQSKLADKLPRISVGNTILDLVVIGCGPAGLALAAESAKLGLSVGLIGPDLPFTNNYGVWEDEFRDLGLEGCIEHVWGDTIVYLDDNDPILIGRAYGRVSRYLLHEELVKRCVESGVSYLSSKVERIIESTNGHSLIECEQNVVVPCRLATVASGAASGKLLQYEVGGPRVSVQTAYGMEVEVENNPYDPNLMVFMDYRDYVKPKVQCLEAQYPTFLYAMPMSPTRVFFEETCLASKDAMPFDILKKKLISRLETMGVRVIKTYEEVEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPKYASVIANILKQGHSRDKLSRSWSTENISMLAWNTLWPQERKRQRAFFLFGLALILQLDIDGIRTFFHTFFRLPTWMWQGFLGSTLSSADLALFAFYMFVIAPNNMRMCLVRHLLSDPTGATMI 525    
BLAST of DCAR_028276 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6J0ZNX0 ((lycopene epsilon cyclase, chloroplastic {ECO:0000313|RefSeq:XP_021276527.1}))

HSP 1 Score: 855.514 bits (2209), Expect = 0.000e+0
Identity = 422/524 (80.53%), Postives = 449/524 (85.69%), Query Frame = 0
 
Query:    5 CIGGRNFTTMAVFSTCPTWRRPRRKRLRRSNVKVSCGRKS---EMRCVKEVS-SCVAVVEDEEFADKEDFVKAGGSELLFVQMQRNKAMDTQSKLAHKLPRIPIRDSVLDLVVIGCGPAGLALAAESAKLGLRVGLIGPDLPFTNNYGVWEDEFIDLGLEGCIEHVWRDTIVYLDDGDPIMIGRAYGRVSRHLLHEELLKRCVESGVSYLSSKVEKIIEAGDGHSLVECENNIVIPCRLATVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFMDYRDYTKQKVPGVEAEYPTFLYVMPMSPTRIFFEETCLASKDAMPFDLLKKKLMSRLQTMGIRVAKTYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYAAVIANILKSSQMNGMINYGRYTENISMQAWKTLWPQERKRQRAFFLFGLALILQLDIDGIRTFFQTFFRLPTWMWQGFLGSSLSSVDLVLFAFYMFIIAPHHLRMSLVRHLLSDPTGATML 524
            C+G R F  MAV STC  WR  RR  L ++   +S   +    ++R     S SCVAV ED  FAD+EDF+KAGGSELLFVQMQ+NK MD QSKLA KLP I I D+VLDLVVIGCGPAGLALAAESAK GL VGLIGPDLPFTNNYGVWEDEF DLGLEGCIEHVWRDTIVYLDD  PIMIGRAYGRVSRH LHEELL+RCVESGV YLSSKVE IIEA DGH+LV CE+N V+PCRL TVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVF  YRDY KQ+VPG EA+YPTFLY MP+S TR+FFEETCLASKDAMPFDLLKKKLMSRL+TMGIR+ K YEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYA+VIANILK     GM    R   NISMQAWKTLWPQERKRQRAFFLFGLALILQLDI GIRTFF TFFRLP+WMWQGFLGS+LSS DLVLFAFYMF+ AP+ LRM LVRHLLSDPTGATM+
Sbjct:    5 CVGARYFAAMAV-STCDAWRS-RRGNLMKTKTALSNNHRFISFKVRASGAGSKSCVAVKED--FADEEDFIKAGGSELLFVQMQQNKEMDKQSKLADKLPPISIGDNVLDLVVIGCGPAGLALAAESAKFGLNVGLIGPDLPFTNNYGVWEDEFKDLGLEGCIEHVWRDTIVYLDDDKPIMIGRAYGRVSRHSLHEELLRRCVESGVMYLSSKVETIIEATDGHNLVACEHNRVVPCRLVTVASGAASGKLLQYEVGGPRVSVQTAYGVEVEVENNPYDPSLMVFAIYRDYAKQEVPGSEAQYPTFLYAMPLSSTRVFFEETCLASKDAMPFDLLKKKLMSRLETMGIRILKVYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPNYASVIANILKKDHSKGMRTSERNNGNISMQAWKTLWPQERKRQRAFFLFGLALILQLDIGGIRTFFHTFFRLPSWMWQGFLGSNLSSADLVLFAFYMFVTAPNDLRMCLVRHLLSDPTGATMI 524    
Match NameStatsDescription
Q2VEX5E-Value: 0.000e+0, PID: 99.05(Putative lycopene epsilon cyclase {ECO:0000313|EM... [more]
A0A5J4ZWV0E-Value: 0.000e+0, PID: 80.95(Uncharacterized protein {ECO:0000313|EMBL:KAA8523... [more]
A0A5B6YMW7E-Value: 0.000e+0, PID: 80.80(Uncharacterized protein {ECO:0000313|EMBL:MPA3313... [more]
A0A2R6R480E-Value: 0.000e+0, PID: 78.86(Lycopene epsilon cyclase {ECO:0000313|EMBL:PSS207... [more]
A0A2C9UAD9E-Value: 0.000e+0, PID: 80.30(Uncharacterized protein {ECO:0000313|EMBL:OAY2709... [more]
A0A6P5ZFW6E-Value: 0.000e+0, PID: 79.92(lycopene epsilon cyclase, chloroplastic isoform X... [more]
A0A061GLV2E-Value: 0.000e+0, PID: 80.26(Lycopene beta/epsilon cyclase protein {ECO:000031... [more]
A0A067KCI7E-Value: 0.000e+0, PID: 79.96(Uncharacterized protein {ECO:0000313|EMBL:KDP3384... [more]
A0A7J7G232E-Value: 0.000e+0, PID: 79.32(Uncharacterized protein {ECO:0000313|EMBL:KAF5934... [more]
A0A6J0ZNX0E-Value: 0.000e+0, PID: 80.53(lycopene epsilon cyclase, chloroplastic {ECO:0000... [more]

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Analysis: 
NameDescription

An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4.

There are a few different naming schemes for this assembly. First there is the
Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below.
These sequences can be viewed in JBrowse here.

Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0

LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926.

Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence.

RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records.

The NCBI Sequence report lists the correspondences between the various naming methods

Link to the LNRQ01000000.1 master record at NCBI

Raw Reads: Link to SRA accessions used for the genome assembly

This genome is available in the CarrotOmics Blast Search

This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions.

For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins.

Data from this analysis can be viewed in JBrowse here.

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