DCAR_028247

Resource Type: 
Polypeptide
Name: 
DCAR_028247
Identifier: 
DCAR_028247.mRNA-protein
Sequence: 
MEAVDLSLHVLDNSVAEDPVPVDSVLGSNCDVIEASKDGDEGNEGLVDPG
CSPAGIAMQVPGSPRLSPLLHSCDSDSDLSRKRKADEGEMCVGIEFELDQ
LEGIAVERESEGIVSGGVSGCVSSGRVLRSRKVEMSPALTQSRERRTQGR
KGIEDGLVSSSGKVLRSGKVAVSTVGLKRDDEDESGLIEFGDDQEGRQTP
VKVNNRPKAGNGKGSDSSSSKAHKQKSVAFFVGEPVPDVEAREKWNWRYQ
LKVMKDMASFHDKKRLFYSTLENDNPLDCLISKVNVIEITREPGFQLNAN
QSTCFYYDMEYCIEYSTFRNLLTDNSVMRHAISSLNNTDTFRTNASASTP
SLEEDLSNCRSQNPDFALLDLYAGCGGMSTGLCLGAELSGIKLVTKWAVD
YQKSACDSLILNHPETQVRNMPAEDFLGLLKEWEKLCNLYALAFDDSDTI
ESKISTDGLEDVEVPRGEYEVSSFVDITYGTISKSGKQGLKFKDFVRSGS
KSKILPVPGDVDVICGGPPCQGISGYNRFRNVDAPLTDERNHQIVVFMDI
VKFLKPKYVLMENVTDILKFDKGSLGRYALSRMVDMKYQARLGIMPAGCY
GLPQFRLRVFLWGALPSEKLPQFPLPTHDVVVKFWPPPEFEQNTVAYNEG
QPRDLEEAAILRDAISDLPAVTSQETREEMSYEMHPETEFQKYIRLSKYE
ITGYRSNIPESETPVLYDHRSYQLSEDDYLRICLIPRRKGANFRDLPGVV
VDQDNKVCRDPTKELEMLPSGKPLVPDYVFTFEKGKPFARLWWDETVGTV
VTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGN
AVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKFPYLVPERTEKPFARLW
WDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVK
ERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKFPYLVPERT
EKLESEH*
Sequence Length: 
1008
Sequence Checksum: 
04ac723141abdba458dec9caa78e4951
View location in JBrowse: 
Relationship: 
There is 1 relationship.
Relationships
The polypeptide, DCAR_028247, derives from mRNA, DCAR_028247.
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Blast Results: 
The following BLAST results are available for this feature:
BLAST of DCAR_028247 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
BLAST of DCAR_028247 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5J5A207 ((Uncharacterized protein {ECO:0000313|EMBL:KAA8525105.1}))

HSP 1 Score: 942.184 bits (2434), Expect = 0.000e+0
Identity = 468/690 (67.83%), Postives = 533/690 (77.25%), Query Frame = 0
 
Query:  242 REKWNWRYQLKVMKDMASFHDKKRLFYSTLENDNPLDCLISKVNVIEITREPGFQLNANQSTCFYYDMEYCIEYSTFRNLLTDNSVMRHAISSLNNTDTFRTNASASTPSLEEDLSNCRSQNPDFALLDLYAGCGGMSTGLCLGAELSGIKLVTKWAVDYQKSACDSLILNHPETQVRNMPAEDFLGLLKEWEKLCNLY-------ALAFDDSDTIESKISTDGLEDVEVPRGEYEVSSFVDITYGTISKSGKQGLKFK---------------------------DFVRSGSKSKILPVPGDVDVICGGPPCQGISGYNRFRNVDAPLTDERNHQIVVFMDIVKFLKPKYVLMENVTDILKFDKGSLGRYALSRMVDMKYQARLGIMPAGCYGLPQFRLRVFLWGALPSEKLPQFPLPTHDVVVKFWPPPEFEQNTVAYNEGQPRDLEEAAILRDAISDLPAVTSQETREEMSYEMHPETEFQKYIRLSKYEITGYR-SNIPESETPVLYDHRSYQLSEDDYLRICLIPRRKGANFRDLPGVVVDQDNKVCRDPTKELEMLPSGKPLVPDYVFTFEKGK---PFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKFPYLVPERTE 893
            R +W +R Q  VMK+ A+FHDK+RLF+ST+ NDN LDC+ISKV+VI++    G +LN+  S+ FYYDMEYC+EYSTFRNL T+NSV      S N+ +TF T+ +  TPS  +D+ +C     + ALLDLY+GCGGMSTGLCLGA+LS + LVT+WAVD+ KSACDSL LNHPETQVRN  AEDFL LLK WEKLC  Y        L     DT E+K +     DV+ P  EYEVS  VDI YG  SK+GK+GLKFK                           DFVRS  KSKILP+PGDVDVICGGPPCQGISGYNRFRNVD PL DERN QIVVFMDIV+FLKPKYVLMENVTDIL+FDK SLGRYALSR+V MKYQARLG + AGCYGLPQFRLRVFLWGALPSE+LPQFPLPTHDVVV++WPPPEFE+NTVAY+EGQPR+LEEA ILRDA SDLPAVTS E REEM YEM PETEFQKYIRL+KYE+ G+  S + E +  +LYDHR YQLSEDDYLR+C IP +KGANFRDLPGVVV  DN   RDPT E+ MLPSGKP+VPDY FT E+GK   PFARLWWDETV TVVTFP+ H+ A+LHPEQDRVLTLRE+ARLQGFPD+YRFCGTVKERYCQVGNAVAV V+RALGY LGMAF+K   D PL+ LPPKF +  P   E
Sbjct:  206 RVQWFYRAQDTVMKEEAAFHDKRRLFFSTVTNDNLLDCIISKVSVIQVAPTLGLELNSIPSSNFYYDMEYCVEYSTFRNLPTENSVESRYSCSPNDIETFHTSITM-TPS--KDMPSCEPHKAELALLDLYSGCGGMSTGLCLGAKLSNVNLVTRWAVDFDKSACDSLKLNHPETQVRNDRAEDFLELLKGWEKLCKWYVFNDLERTLQSRSKDTREAKSNKKSQSDVKTPAREYEVSCLVDICYGDPSKTGKRGLKFKVRWKGYGSSEDTWEPIEGLSNCQQRIQDFVRSRLKSKILPLPGDVDVICGGPPCQGISGYNRFRNVDTPLADERNRQIVVFMDIVEFLKPKYVLMENVTDILRFDKASLGRYALSRLVHMKYQARLGTIAAGCYGLPQFRLRVFLWGALPSERLPQFPLPTHDVVVRYWPPPEFERNTVAYDEGQPRELEEAVILRDAFSDLPAVTSHEVREEMPYEMPPETEFQKYIRLTKYEMMGFALSGVTEPKNSILYDHRPYQLSEDDYLRVCQIPHKKGANFRDLPGVVVGTDNVAHRDPTNEI-MLPSGKPMVPDYAFTLEQGKSKRPFARLWWDETVATVVTFPNLHSQAALHPEQDRVLTLREYARLQGFPDFYRFCGTVKERYCQVGNAVAVPVARALGYTLGMAFRKLTGDEPLMTLPPKFSFQRPSNEE 891    

HSP 2 Score: 186.037 bits (471), Expect = 9.234e-46
Identity = 93/166 (56.02%), Postives = 112/166 (67.47%), Query Frame = 0
 
Query:  844 RYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKFPYLVPE--------RTEKPFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKFPYLVPERTE 1001
            R CQ+ +          G  +G       D    + LP   P +VP+        ++++PFARLWWDETV TVVTFP+ H+ A+LHPEQDRVLTLRE+ARLQGFPD+YRFCGTVKERYCQVGNAVAV V+RALGY LGMAF+K   D PL+ LPPKF +  P   E
Sbjct:  727 RVCQIPHKKGANFRDLPGVVVGTDNVAHRDPTNEIMLPSGKP-MVPDYAFTLEQGKSKRPFARLWWDETVATVVTFPNLHSQAALHPEQDRVLTLREYARLQGFPDFYRFCGTVKERYCQVGNAVAVPVARALGYTLGMAFRKLTGDEPLMTLPPKFSFQRPSNEE 891    

HSP 3 Score: 64.6994 bits (156), Expect = 8.768e-8
Identity = 47/134 (35.07%), Postives = 66/134 (49.25%), Query Frame = 0
 
Query:  122 VSSGRVLRSRKVEMSPALTQSRERRTQGRKGIEDGLVSS---SGKVLRSGKVAVSTVGLKRDDEDESGLIEFGDDQEGRQTPVKVNNRPKAGNGKGSDSSSSKAHKQKSVAFFVGEPVPDVEAREKWNWRYQLK 252
            +S  + LRSR +     +  S + +T  +  I  G   S   S K LRS K+    VG++ + + ES  +E                   +G  +   SS  K  KQKS AFF+G+PVP+VEARE+W WRY LK
Sbjct:   23 ISGEKCLRSRTIPFH--VDDSVDDKTPAKIKITPGSCDSDMLSEKCLRSRKIPFRLVGIE-NVKTESASMEL------------------SGKIETFASSEKKQRKQKSKAFFIGDPVPEVEARERWGWRYDLK 135    
BLAST of DCAR_028247 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2R6Q374 (DNA (cytosine-5-)-methyltransferase {ECO:0000256|ARBA:ARBA00011975} (2.1.1.37 {ECO:0000256|ARBA:ARBA00011975}))

HSP 1 Score: 897.501 bits (2318), Expect = 0.000e+0
Identity = 442/679 (65.10%), Postives = 519/679 (76.44%), Query Frame = 0
 
Query:  242 REKWNWRYQLKVMKDMASFHDKKRLFYSTLENDNPLDCLISKVNVIEITREPGFQLNANQSTCFYYDMEYCIEYSTFRNLLTDNSVMRHAISSLNNTDTFRTNASASTPSLEEDLSNCRSQNPDFALLDLYAGCGGMSTGLCLGAELSGIKLVTKWAVDYQKSACDSLILNHPETQVRNMPAEDFLGLLKEWEKLCNLYALAFDDSDTIES------KISTDGLEDVEVPRGEYEVSSFVDITYGTISKSGKQGLKFK---------------------------DFVRSGSKSKILPVPGDVDVICGGPPCQGISGYNRFRNVDAPLTDERNHQIVVFMDIVKFLKPKYVLMENVTDILKFDKGSLGRYALSRMVDMKYQARLGIMPAGCYGLPQFRLRVFLWGALPSEKLPQFPLPTHDVVVKFWPPPEFEQNTVAYNEGQPRDLEEAAILRDAISDLPAVTSQETREEMSYEMHPETEFQKYIRLSKYEITGYRSNIPESETPVLYDHRSYQLSEDDYLRICLIPRRKGANFRDLPGVVVDQDNKVCRDPTKELEMLPSGKPLVPDYVFTFEKGK---PFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKF 884
            R +W +R +  VM++ A+FHD+ RLFYSTL NDN LDC+ISKVNVI+I    G Q N+   + FYYDMEYC+EYSTFR L T++    + +S  N  + FR   ++++     D+ +      D ALLDLY+GCGGMSTGLCLGA+LS + LVT+WAVD  +SACDSL LNHPETQVRN  AEDFL LLK WEKLC  Y  + +   T ES      ++ ++   D ++  GEYEV   VDI YG   K GK+GL+FK                           DFVR G KSKILP+PGDVDV+CGGPPCQGISGYNRFRN+DAPL+DERNHQIVVFMDIVKFL+PKYVLMENV DIL+FDK SL RYALSR+V MKYQARLG M AGCYGLPQFRLR FL GALPSEKLPQFP PTHDVVVK+WPP EFE+NTVAY+EG+ R+LEEA +LRDAISDLPAV S E RE+M+YE+ PETEFQKYIRL+K E+ G+   + + + P+LYDHR YQLSEDDY+R+C IP++KGANFRDLPGVVV  DN V RDPTKE  MLPSGKP+VPDYVFTFE+GK   PFAR+WWDETV TVVTFPS HN A+LHPEQDRVLT+RE+ARLQGFPD+YRFCG+VK RYCQVGNAVAV V+RALGY+LGMAF+K G   PL+ LPPKF
Sbjct:  370 RVQWFFRAEDTVMQNEAAFHDENRLFYSTLMNDNLLDCIISKVNVIQIAPTLGLQPNSIPPSDFYYDMEYCVEYSTFRTLPTES----YNLSIPNGIEGFRDMLTSTS---LRDMPSFEPYKGDLALLDLYSGCGGMSTGLCLGAKLSSVNLVTRWAVDANRSACDSLKLNHPETQVRNETAEDFLELLKGWEKLCQSYVFSNNLDRTAESTVEDTSEVKSNVQSDAKIRGGEYEVLRLVDICYGDHCKRGKRGLEFKVRWKGYSSDDDTWEPIEGLSNCQERIQDFVRRGFKSKILPLPGDVDVLCGGPPCQGISGYNRFRNIDAPLSDERNHQIVVFMDIVKFLRPKYVLMENVVDILRFDKASLARYALSRLVHMKYQARLGTMAAGCYGLPQFRLRAFLLGALPSEKLPQFPRPTHDVVVKYWPPSEFERNTVAYDEGEHRELEEAIVLRDAISDLPAVPSHEIREQMAYEIPPETEFQKYIRLTKDEMMGFAKGVEDGKKPILYDHRPYQLSEDDYIRVCQIPQKKGANFRDLPGVVVGPDNVVLRDPTKEKMMLPSGKPMVPDYVFTFEQGKSKRPFARIWWDETVPTVVTFPSLHNQAALHPEQDRVLTIREYARLQGFPDFYRFCGSVKRRYCQVGNAVAVPVARALGYSLGMAFRKQGGTEPLMTLPPKF 1041    

HSP 2 Score: 180.259 bits (456), Expect = 1.258e-43
Identity = 78/102 (76.47%), Postives = 92/102 (90.20%), Query Frame = 0
 
Query:  891 RTEKPFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKF 992
            ++++PFAR+WWDETV TVVTFPS HN A+LHPEQDRVLT+RE+ARLQGFPD+YRFCG+VK RYCQVGNAVAV V+RALGY+LGMAF+K G   PL+ LPPKF
Sbjct:  940 KSKRPFARIWWDETVPTVVTFPSLHNQAALHPEQDRVLTIREYARLQGFPDFYRFCGSVKRRYCQVGNAVAVPVARALGYSLGMAFRKQGGTEPLMTLPPKF 1041    

HSP 3 Score: 54.6842 bits (130), Expect = 1.071e-4
Identity = 35/90 (38.89%), Postives = 46/90 (51.11%), Query Frame = 0
 
Query:  163 KVLRSGKVAVSTVGLKRDDEDESGLIEFGDDQEGRQTPVKVNNRPKAGNGKGSDSSSSKAHKQKSVAFFVGEPVPDVEAREKWNWRYQLK 252
            K LRS K+    VG      DE+G  +  +D+           RPK    K    S  K + ++    FVGEPVP+ EAREKW+WRY+LK
Sbjct:  228 KSLRSRKIPFRVVG------DENGNTKLAEDR-----------RPKQKT-KTHVPSEEKENAEEITCIFVGEPVPEDEAREKWSWRYELK 299    
BLAST of DCAR_028247 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A4S4E0R5 (Cytosine-specific methyltransferase {ECO:0000256|RuleBase:RU000417} (2.1.1.37 {ECO:0000256|RuleBase:RU000417}))

HSP 1 Score: 886.715 bits (2290), Expect = 0.000e+0
Identity = 447/687 (65.07%), Postives = 517/687 (75.25%), Query Frame = 0
 
Query:  253 VMKDMASFHDKKRLFYSTLENDNPLDCLISKVNVIEITREPGFQLNANQSTCFYYDMEYCIEYSTFRNLLTDNSVMRHAISSLNNTDTFRTNASASTPSLEEDLSNCRSQNPDFALLDLYAGCGGMSTGLCLGAELSGIKLVTKWAVDYQKSACDSLILNHPETQVRNMPAEDFLGLLKEWEKLCNLYALAFDDS-------DTIESKISTDGLEDVEVPRGEYEVSSFVDITYGTISKSGKQGLKFK------------------------------------------DFVRSGSKSKILPVPGDVDVICGGPPCQGISGYNRFRNVDAPLTDERNHQIVVFMDIVKFLKPKYVLMENVTDILKFDKGSLGRYALSRMVDMKYQARLGIMPAGCYGLPQFRLRVFLWGALPSEKLPQFPLPTHDVVVKFWPPPEFEQNTVAYNEGQPRDLEEAAILRDAISDLPAVTSQETREEMSYEMHPETEFQKYIRLSKYEITGY-RSNIPESETPVLYDHRSYQLSEDDYLRICLIPRRKGANFRDLPGVVVDQDNKVCRDPTKELEMLPSGKPLVPDYVFTFEKGK---PFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKFPY 886
            VMK+ A+ HDKKRLFYSTL NDN LDC+ISKV VI++    G QLN+   + FYYDMEYC+EYSTFR LLTD SV    +S  ++ +T    A   TP   E++++C     + ALLDLY+GCGGMSTGLCLGA+LS + LVT+WAVD   SACDSL LNHPETQVRN  AEDFL LLK WEKLC  Y     D+       DT E K + +   DV++  GE+EV   VDI Y    KSGK+GLKFK                                          DFVRSG KSKILP+PGDVDV+CGGPPCQGISG+NR+RN+DAPL DERNHQIVVFMDIVKFL+PKYVLMENV DILKFDK SL RYALSR+V+MKYQARLG M AGCYGLPQFRLRVFLWGALPSEKLPQ+PLPTHDVVV+ WPP EFEQNTVAY+EG+PR+LEE  ILRDAISDLPAVTS E RE+M+Y M PETEFQKYIRL+K E+ G+ +  + +++  +L+DHR YQLSEDDYLR+C IP +KGANFRDLPG+VV  DN V RD TKE  MLPSGKP+VPDYV TFE+GK   PFARLWWDETV TVVTFP+  N A+LHPEQDRVLT+RE+ARLQGFPD+YRFCGTVK+RYCQVGNAVAV V+RALGY+LG+A QK G + PL+ LPPKF +
Sbjct:   16 VMKEEAACHDKKRLFYSTLMNDNLLDCIISKVIVIQVAPTLGLQLNSFPRSDFYYDMEYCVEYSTFRTLLTDYSVESCDLSLRDDIETLMITA---TPL--EEMTHCEPYKAELALLDLYSGCGGMSTGLCLGAKLSSVNLVTRWAVDADNSACDSLKLNHPETQVRNENAEDFLELLKGWEKLCQWYVFNNLDTTLKSREDDTREVKSNLNSPSDVKISAGEFEVLRLVDICYEDQGKSGKRGLKFKVRWKGYSSSEDTWEPIEALSDLVLHYYLQICICLSNCQERIQDFVRSGFKSKILPLPGDVDVLCGGPPCQGISGFNRYRNIDAPLDDERNHQIVVFMDIVKFLRPKYVLMENVVDILKFDKASLARYALSRLVNMKYQARLGTMAAGCYGLPQFRLRVFLWGALPSEKLPQYPLPTHDVVVRNWPPLEFEQNTVAYDEGEPRELEEPIILRDAISDLPAVTSHEIREQMTYGMPPETEFQKYIRLTKDEMMGFAQKGVKDAKKAILHDHRPYQLSEDDYLRVCQIPHKKGANFRDLPGLVVGHDNMVRRDATKEQMMLPSGKPMVPDYVLTFEQGKSKRPFARLWWDETVPTVVTFPNLRNQAALHPEQDRVLTIREYARLQGFPDFYRFCGTVKKRYCQVGNAVAVPVARALGYSLGIAVQKLGGNEPLMTLPPKFTF 697    

HSP 2 Score: 175.637 bits (444), Expect = 5.518e-43
Identity = 77/104 (74.04%), Postives = 93/104 (89.42%), Query Frame = 0
 
Query:  891 RTEKPFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKFPY 994
            ++++PFARLWWDETV TVVTFP+  N A+LHPEQDRVLT+RE+ARLQGFPD+YRFCGTVK+RYCQVGNAVAV V+RALGY+LG+A QK G + PL+ LPPKF +
Sbjct:  594 KSKRPFARLWWDETVPTVVTFPNLRNQAALHPEQDRVLTIREYARLQGFPDFYRFCGTVKKRYCQVGNAVAVPVARALGYSLGIAVQKLGGNEPLMTLPPKFTF 697    
BLAST of DCAR_028247 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5N6R441 (Cytosine-specific methyltransferase {ECO:0000256|RuleBase:RU000417} (2.1.1.37 {ECO:0000256|RuleBase:RU000417}))

HSP 1 Score: 886.33 bits (2289), Expect = 0.000e+0
Identity = 435/683 (63.69%), Postives = 517/683 (75.70%), Query Frame = 0
 
Query:  242 REKWNWRYQLKVMKDMASFHDKKRLFYSTLENDNPLDCLISKVNVIEITREPGFQLNANQSTCFYYDMEYCIEYSTFRNLLTDNSVMRHAISSLNNTDTFRTNASASTPSLEEDLSNCRSQNPDFALLDLYAGCGGMSTGLCLGAELSGIKLVTKWAVDYQKSACDSLILNHPETQVRNMPAEDFLGLLKEWEKLCNLYALAFDDSDTIESKIST--------DGLEDVEVPRGEYEVSSFVDITYGTISKSGKQGLKFK---------------------------DFVRSGSKSKILPVPGDVDVICGGPPCQGISGYNRFRNVDAPLTDERNHQIVVFMDIVKFLKPKYVLMENVTDILKFDKGSLGRYALSRMVDMKYQARLGIMPAGCYGLPQFRLRVFLWGALPSEKLPQFPLPTHDVVVKFWPPPEFEQNTVAYNEGQPRDLEEAAILRDAISDLPAVTSQETREEMSYEMHPETEFQKYIRLSKYEITGYRSNIPESETPVLYDHRSYQLSEDDYLRICLIPRRKGANFRDLPGVVVDQDNKVCRDPTKELEMLPSGKPLVPDYVFTFEKGK---PFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKFPY 886
            R +W +R +  V+K+  +FHDK+RLFYST+ NDN +DC+I+KVNV +IT   G +LN+   + F+ DMEYC++YSTFR+ +TD+SV  H +SS    D+  +    +TP+  E + NC +     ALLDL++GCGGMSTGLCLGA+LSG+ L T WAVD  +SAC+SL LNH ET VRN  AEDFL LLKEWEKLC  Y +  D   T +S+  +        + L D EVP GEYEVS  VDI YG  +K+GK GL FK                            FV+ G K KILP+PGDVDVICGGPPCQGISGYNRFRN D+PL D+RNHQIVVFMDIVK+LKPKYVLMENV DIL+FDK SLGRYALSR+V M YQARLG + AGCYGLPQFRLRVFLWGA PSEKLPQFPLPTHDV+V++WPPPEFE+NTVAY+EGQP  LE+A +L+DAISDLPAVT+ ETRE MSY+  PETEFQ+YIR ++YE+TG   +      P L+DHR Y LSEDDYLR+C IP+RKGANFRDLPGVVV  DN V RDP KE ++LPSGK LVPDY FTFE+GK   PFARLWWDETV TVVTFPSCHN A+LHPEQDRVLT+RE+ARLQGFPDYYRFCGT+KERYCQ+GNAV+V V+RALGYALG++F+K   D PL  LPP+F +
Sbjct:  404 RIQWFYRVEDTVIKEEGAFHDKRRLFYSTVMNDNLIDCIIAKVNVTQITPRVGLRLNSIPPSDFFCDMEYCVDYSTFRSFVTDDSVKSHDVSS---PDSVESVCPKATPTFLEKIFNCETFKDKLALLDLFSGCGGMSTGLCLGAKLSGVNLATSWAVDSDRSACESLRLNHRETHVRNETAEDFLKLLKEWEKLCKRYKVN-DLGRTHQSRAKSLRAARCNGNSLNDDEVPTGEYEVSRLVDICYGDPNKTGKHGLYFKVHWKGYSTSKDTWEPIEGLRNCQEMVQKFVKHGLKLKILPLPGDVDVICGGPPCQGISGYNRFRNHDSPLADDRNHQIVVFMDIVKYLKPKYVLMENVVDILRFDKASLGRYALSRLVHMNYQARLGTIAAGCYGLPQFRLRVFLWGAHPSEKLPQFPLPTHDVIVRYWPPPEFERNTVAYDEGQPHKLEKAVVLQDAISDLPAVTNFETREAMSYQKPPETEFQRYIRSTRYEMTGSTLSGSLETKPSLFDHRPYALSEDDYLRVCQIPKRKGANFRDLPGVVVGADNVVRRDP-KEQKLLPSGKALVPDYAFTFEQGKSRRPFARLWWDETVPTVVTFPSCHNQAALHPEQDRVLTIREYARLQGFPDYYRFCGTIKERYCQIGNAVSVPVARALGYALGLSFRKLSRDEPLTTLPPEFSH 1081    

HSP 2 Score: 183.726 bits (465), Expect = 1.087e-44
Identity = 84/124 (67.74%), Postives = 100/124 (80.65%), Query Frame = 0
 
Query:  879 KLPPKFPYLVPE--------RTEKPFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKFPY 994
            KL P    LVP+        ++ +PFARLWWDETV TVVTFPSCHN A+LHPEQDRVLT+RE+ARLQGFPDYYRFCGT+KERYCQ+GNAV+V V+RALGYALG++F+K   D PL  LPP+F +
Sbjct:  958 KLLPSGKALVPDYAFTFEQGKSRRPFARLWWDETVPTVVTFPSCHNQAALHPEQDRVLTIREYARLQGFPDYYRFCGTIKERYCQIGNAVSVPVARALGYALGLSFRKLSRDEPLTTLPPEFSH 1081    

HSP 3 Score: 44.669 bits (104), Expect = 1.331e-1
Identity = 26/72 (36.11%), Postives = 37/72 (51.39%), Query Frame = 0
 
Query:  184 ESGLIEFGDDQEGRQTPVKVNNRPKAGNGKGSDSSSSKAHKQKSVAFFVGEPVPDVEAREKWNWRYQLKVMK 255
            E  LIE  +  E +Q P K          K   S   K  K K+ + F+G+P+ D EARE+W WRY++K  +
Sbjct:  275 EYSLIELSESDE-KQPPKKF---------KTPASLEYKQKKYKNRSSFIGDPILDHEARERWRWRYEMKSQR 336    
BLAST of DCAR_028247 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A251P2J6 (Cytosine-specific methyltransferase {ECO:0000256|RuleBase:RU000417} (2.1.1.37 {ECO:0000256|RuleBase:RU000417}))

HSP 1 Score: 885.945 bits (2288), Expect = 0.000e+0
Identity = 452/803 (56.29%), Postives = 548/803 (68.24%), Query Frame = 0
 
Query:  163 KVLRSGKVAVSTVGLKRDDEDESGLIEFGDDQEGRQTPVKVNNRPKAGNGKGSDSSSSKAHKQKSV--AFFVGEPVPDVEAREKWNWRYQLK----------------------------------------------VMKDMASFHDKKRLFYSTLENDNPLDCLISKVNVIEITREPGFQLNANQSTCFYYDMEYCIEYSTFRNLLTDNSVMRHAISSLNNTDTFRTNASASTPSLEEDLSNCRSQNPDFALLDLYAGCGGMSTGLCLGAELSGIKLVTKWAVDYQKSACDSLILNHPETQVRNMPAEDFLGLLKEWEKLCNLYALA-FDDSDTIESKISTDGLEDVEVPRGEYEVSSFVDITYGTISKSGKQGLKFK---------------------------DFVRSGSKSKILPVPGDVDVICGGPPCQGISGYNRFRNVDAPLTDERNHQIVVFMDIVKFLKPKYVLMENVTDILKFDKGSLGRYALSRMVDMKYQARLGIMPAGCYGLPQFRLRVFLWGALPSEKLPQFPLPTHDVVVKFWPPPEFEQNTVAYNEGQPRDLEEAAILRDAISDLPAVTSQETREEMSYEMHPETEFQKYIRLSKYEITGYRSNIPESETPVLYDHRSYQLSEDDYLRICLIPRRKGANFRDLPGVVVDQDNKVCRDPTKELEMLPSGKPLVPDYVFTFEKGK---PFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKFPY 886
            K LR  +   + +      E E  LIE  +          V  +PK    K  DSS+++ +KQK+      +G+P+PDVEA+E+W WRYQ+K                                              V+KD+   HDK+RLFYST+ NDN +DC++SKVNV  ++   G + N+  S+ FYYDMEYC++YSTFR+L+TDNS+    + + N+ +     +  ST +L E++ +  + N + A+LDLY GCGGMSTGLCLGA+LS + LVT+WA+D  KSAC+SL LNHPETQVRN  AEDFL LLKEWEKLC  Y +   + +  + SK S       E+   EYEVS  VDI YG  +K+GK G+ FK                           DFVR G K KILP+PGDVDVICGGPPCQGISGYNR+RNVD+PL DERNHQIVVFMDIVKFLKPKYVLMENV DIL+FDK SLGRYALSR+V M YQARLGI+ AGCYGLPQFRLRVFLWGA P E LPQFPLPTHDV++++WPPPEFE+NTVAY+E QPR LEEA +L DAISDLPAVT+ ET EEMSY+  P T+FQ+YIR ++YE+ G   +        LYDHR Y L EDDYLR+C IP+RKGANFRDLPGVVV  DN   RD T++  +LPSGKPLVPDY FT+E+GK   PFARLWWDETV TV+TFP+CH+ A LHPEQDR+LTLRE ARLQGFPDYYRFCGTVKERYCQVGNAVAV V+RALGYALG+A +K   + PL+ LP  F +
Sbjct:  247 KYLRQSRKEATAMFENEGRETECSLIELPE--------TYVKQQPK--KRKTVDSSANRQNKQKNCKSTSLIGDPIPDVEAQERWGWRYQMKSQRSKSQSLKLNEDEEDEIILNVECHFSQAKIGNCIFSLGDCAYIKVIKDVGDVHDKRRLFYSTIMNDNLIDCIVSKVNVKHVSPRVGLKGNSILSSDFYYDMEYCVDYSTFRSLITDNSLKNQNLPAYNSIEAV---SILSTVNLLENMPSSETYNAELAVLDLYCGCGGMSTGLCLGAKLSCVNLVTRWALDSDKSACESLKLNHPETQVRNEAAEDFLELLKEWEKLCRRYTVNDVERTYPLRSKTSKIPKNSNEIATDEYEVSRIVDICYGDPNKTGKHGVNFKVHWKGYSSSEDTWEPIGGLSNCLGSIQDFVRYGKKVKILPLPGDVDVICGGPPCQGISGYNRYRNVDSPLDDERNHQIVVFMDIVKFLKPKYVLMENVVDILRFDKASLGRYALSRLVHMNYQARLGILAAGCYGLPQFRLRVFLWGAHPCENLPQFPLPTHDVIIRYWPPPEFERNTVAYDEDQPRVLEEAVVLHDAISDLPAVTNDETSEEMSYQKPPGTDFQRYIRSTQYEMMGSVLDGTMKTKVSLYDHRPYPLFEDDYLRVCQIPKRKGANFRDLPGVVVGNDNVARRDSTEKHLLLPSGKPLVPDYAFTYEQGKSKRPFARLWWDETVPTVLTFPTCHSQAILHPEQDRILTLRECARLQGFPDYYRFCGTVKERYCQVGNAVAVPVARALGYALGLAVRKLSGNEPLMTLPRNFSH 1036    

HSP 2 Score: 174.096 bits (440), Expect = 1.171e-41
Identity = 89/167 (53.29%), Postives = 111/167 (66.47%), Query Frame = 0
 
Query:  833 DYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLK----LPPKFPYLVPE-RTEKPFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKFPY 994
            DY R C   K +     +   V V         +A + S +   LL     L P + +   + ++++PFARLWWDETV TV+TFP+CH+ A LHPEQDR+LTLRE ARLQGFPDYYRFCGTVKERYCQVGNAVAV V+RALGYALG+A +K   + PL+ LP  F +
Sbjct:  875 DYLRVCQIPKRKGANFRDLPGVVVGND-----NVARRDSTEKHLLLPSGKPLVPDYAFTYEQGKSKRPFARLWWDETVPTVLTFPTCHSQAILHPEQDRILTLRECARLQGFPDYYRFCGTVKERYCQVGNAVAVPVARALGYALGLAVRKLSGNEPLMTLPRNFSH 1036    
BLAST of DCAR_028247 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A7J0GCT9 (Cytosine-specific methyltransferase {ECO:0000256|RuleBase:RU000417} (2.1.1.37 {ECO:0000256|RuleBase:RU000417}))

HSP 1 Score: 874.389 bits (2258), Expect = 0.000e+0
Identity = 435/675 (64.44%), Postives = 513/675 (76.00%), Query Frame = 0
 
Query:  221 KAHKQKSVAFFVGEPVPDVEAREKWNWRYQLKVMKDMASFHDKKRLFYSTLENDNPLDCLISKVNVIEITREPGFQLNANQSTCFYYDMEYCIEYSTFRNLLTDNSVMRHAISSLNNTDTFRTNASASTPSLEEDLSNCRSQNPDFALLDLYAGCGGMSTGLCLGAELSGIKLVTKWAVDYQKSACDSLILNHPETQVRNMPAEDFLGLLKEWEKLCNLYALAFDDSDTIES------KISTDGLEDVEVPRGEYEVSSFVDITYGTISKSGKQGLKFKDFVRSGSKSKILPVPGDVDVICGGPPCQGISGYNRFRNVDAPLTDERNHQIVVFMDIVKFLKPKYVLMENVTDILKFDKGSLGRYALSRMVDMKYQARLGIMPAGCYGLPQFRLRVFLWGALPSEKLPQFPLPTHDVVVKFWPPPEFEQNTVAYNEGQPRDLEEAAILRDAISDLPAVTSQETREEMSYEMHPETEFQKYIRLSKYEITGYRSNIPESETPVLYDHRSYQLSEDDYLRICLIPRRKGANFRDLPGVVVDQDNKVCRDPTKELEMLPSGKPLVPDYVFTFEKGK---PFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKFPY 886
            K H  K + FF      D   R +W +R +  VM++ A+FHD+ RLFYSTL NDN LDC+ISKVNVI+I    G Q N+   + FYYDMEYC+EYSTFR L T++    + +S  N  + FR   ++++     D+ +      D ALLDLY+GCGGMSTGLCLGA+LS + LVT+WAVD  +SACDSL LNHPETQVRN  A+DFL LLK WEKLC  Y  + +   T ES      ++ ++   D ++  GEYEV   VDI YG   K GK+GL+FK               GDVDV+CGGPPCQGISGYNRFRN+DAPL+DERNHQIVVFMDIVKFL+PKYVLMENV DIL+FDK SL RYALSR+V MKYQARLG M AGCYGLPQFRLR FL GALPSEKLPQFP PTHDVVVK+WPP EFE+NTVAY+EG+ R+LEEA +LRDAISDLPAV S E RE+M YE+ PETEFQKYIRL+K E+ G+   + + + P+LYDHR YQLSEDDY+R+C IP++KGANFRDLPGVVV  DN V RDPTKE  MLPSGKP+VPDYVFTFE+GK   PFAR+WWDETV TVVTFPS HN A+LHPEQDRVLT+RE+ARLQGFPD+YRFCG+VK RYCQVGNAVAV V+RALGY+LGMAF+K G   PL+ LPPKF +
Sbjct:  104 KKHVGKILEFFKTTDGEDY-FRVQWFFRAEDTVMQNEAAFHDENRLFYSTLMNDNLLDCIISKVNVIQIAPTLGLQPNSIPPSDFYYDMEYCVEYSTFRTLPTES----YNLSIPNGIEGFRDMLTSTS---LRDMPSFEPYKGDLALLDLYSGCGGMSTGLCLGAKLSSVNLVTRWAVDANRSACDSLKLNHPETQVRNETADDFLELLKGWEKLCQSYVFSNNLDRTAESTVEDTSEVKSNVQSDAKIRGGEYEVLRLVDICYGDHCKRGKRGLEFK---------------GDVDVLCGGPPCQGISGYNRFRNIDAPLSDERNHQIVVFMDIVKFLRPKYVLMENVVDILRFDKASLARYALSRLVHMKYQARLGTMAAGCYGLPQFRLRAFLLGALPSEKLPQFPRPTHDVVVKYWPPSEFERNTVAYDEGEHRELEEAIVLRDAISDLPAVPSHEIREQMVYEIPPETEFQKYIRLTKDEMMGFAKGVEDGKKPILYDHRPYQLSEDDYIRVCQIPQKKGANFRDLPGVVVGPDNVVLRDPTKEKMMLPSGKPMVPDYVFTFEQGKSKRPFARIWWDETVPTVVTFPSLHNQAALHPEQDRVLTIREYARLQGFPDFYRFCGSVKRRYCQVGNAVAVPVARALGYSLGMAFRKQGGTEPLMTLPPKFAF 755    

HSP 2 Score: 180.259 bits (456), Expect = 3.197e-44
Identity = 78/104 (75.00%), Postives = 93/104 (89.42%), Query Frame = 0
 
Query:  891 RTEKPFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKFPY 994
            ++++PFAR+WWDETV TVVTFPS HN A+LHPEQDRVLT+RE+ARLQGFPD+YRFCG+VK RYCQVGNAVAV V+RALGY+LGMAF+K G   PL+ LPPKF +
Sbjct:  652 KSKRPFARIWWDETVPTVVTFPSLHNQAALHPEQDRVLTIREYARLQGFPDFYRFCGSVKRRYCQVGNAVAVPVARALGYSLGMAFRKQGGTEPLMTLPPKFAF 755    
BLAST of DCAR_028247 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P9E470 ((DNA (Cytosine-5)-methyltransferase CMT2-like isoform X2 {ECO:0000313|RefSeq:XP_035542186.1}))

HSP 1 Score: 870.537 bits (2248), Expect = 0.000e+0
Identity = 427/674 (63.35%), Postives = 508/674 (75.37%), Query Frame = 0
 
Query:  242 REKWNWRYQLKVMKDMASFHDKKRLFYSTLENDNPLDCLISKVNVIEITREPGFQLNANQSTCFYYDMEYCIEYSTFRNLLTDNSVMRHAISSLNNTDTFRTNASASTPSLEEDLSNCRSQNPDFALLDLYAGCGGMSTGLCLGAELSGIKLVTKWAVDYQKSACDSLILNHPETQVRNMPAEDFLGLLKEWEKLCNLYALAFDDSDTIESKISTDGLEDVE-VPRGEYEVSSFVDITYGTISKSGKQGLKFK---------------------------DFVRSGSKSKILPVPGDVDVICGGPPCQGISGYNRFRNVDAPLTDERNHQIVVFMDIVKFLKPKYVLMENVTDILKFDKGSLGRYALSRMVDMKYQARLGIMPAGCYGLPQFRLRVFLWGALPSEKLPQFPLPTHDVVVKFWPPPEFEQNTVAYNEGQPRDLEEAAILRDAISDLPAVTSQETREEMSYEMHPETEFQKYIRLSKYEITGYRSNIPESETPVLYDHRSYQLSEDDYLRICLIPRRKGANFRDLPGVVVDQDNKVCRDPTKELEMLPSGKPLVPDYVFTFEKGK---PFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKF 884
            R +W ++ +  V+K+  +FH+K+RLFYST+ NDN +DC+ISKV+V +IT   G  LN    + FY+DMEYC++YSTFR+L+ DNSV  H +SS +      T A   TP+  +++ N  +     ALLDL++GCGGMSTG+CLGA+L+G+ L  +WA+D   SAC+SL LNHPET VRN  A DFL LL EWEKLC  Y L      T  S++ T G +    +P GEYEVS  VDI YG  +K+GK+GL FK                           DFV++G KSKILP+PGDVDVICGGPPCQGISGYNR+RN+D+PL+D+RN QIVVFMDIV FLKPK+VLMENV DIL+FDK SLGRYALSR+V M YQARLG + AGCYGLPQFRLRVFLWGA PSEKLPQFPLPTHDV+V++WPPPEFE+NTVAY E QPR+LEEA +LRDAISDLPAVT+ E REEMS E  PET+FQ+YIR ++YE+TG   N        LYDHR Y LSEDDYLR+C IP+RKGANFRDLPGVVV  DN   RD T E ++LPSGK LVPDY FTFE+GK   PFARLWWDETV TVVT PSCHN A+LHPEQDRVLT+RE+ARLQGFPDYYR CGT+KERYCQVGNAVA+ V+RALGYALG+AF+K   D PL+ LPPKF
Sbjct:  537 RVQWFYKVEDTVIKEEGAFHNKRRLFYSTIMNDNLIDCIISKVSVTQITPRVGRILNFILPSDFYFDMEYCVDYSTFRSLVIDNSVRSHDVSSPDGIKPVDTRA---TPTFSQNVPNSETYEAKLALLDLFSGCGGMSTGMCLGAKLTGVNLAMRWALDSDGSACESLKLNHPETLVRNETAADFLELLMEWEKLCGHYRLN-GAGRTHPSRVMTSGADSCNGIPTGEYEVSRLVDICYGDPNKTGKRGLNFKVHWKGYSTSEDTWEPIEGLSNCQERIQDFVQNGLKSKILPLPGDVDVICGGPPCQGISGYNRYRNIDSPLSDDRNRQIVVFMDIVTFLKPKFVLMENVVDILRFDKASLGRYALSRLVHMNYQARLGTIAAGCYGLPQFRLRVFLWGAHPSEKLPQFPLPTHDVIVRYWPPPEFERNTVAYEEDQPRELEEALVLRDAISDLPAVTNFEIREEMSREKPPETDFQRYIRSTRYEMTGSALNDSLETKHSLYDHRPYALSEDDYLRVCQIPKRKGANFRDLPGVVVGADNVARRDRT-EQKLLPSGKSLVPDYAFTFEQGKSRRPFARLWWDETVPTVVTSPSCHNQAALHPEQDRVLTIREYARLQGFPDYYRLCGTIKERYCQVGNAVAIPVARALGYALGLAFRKLSRDEPLMTLPPKF 1205    

HSP 2 Score: 184.882 bits (468), Expect = 6.034e-45
Identity = 92/157 (58.60%), Postives = 108/157 (68.79%), Query Frame = 0
 
Query:  844 RYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKFPYLVPE--------RTEKPFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKF 992
            R CQ+            G  +G A   +  D    KL P    LVP+        ++ +PFARLWWDETV TVVT PSCHN A+LHPEQDRVLT+RE+ARLQGFPDYYR CGT+KERYCQVGNAVA+ V+RALGYALG+AF+K   D PL+ LPPKF
Sbjct: 1050 RVCQIPKRKGANFRDLPGVVVG-ADNVARRDRTEQKLLPSGKSLVPDYAFTFEQGKSRRPFARLWWDETVPTVVTSPSCHNQAALHPEQDRVLTIREYARLQGFPDYYRLCGTIKERYCQVGNAVAIPVARALGYALGLAFRKLSRDEPLMTLPPKF 1205    

HSP 3 Score: 52.373 bits (124), Expect = 5.231e-4
Identity = 36/114 (31.58%), Postives = 52/114 (45.61%), Query Frame = 0
 
Query:  169 KVAVSTVGLKRDDEDESGLI---EFGDDQEG---RQTPVKVNNRPKAGNGK---------------------GSDSSSSKAHKQKSVAFFVGEPVPDVEAREKWNWRYQLKVMK 255
            K+  + + L   DE  S +I       D +G   RQ+P+   +   A NGK                       DSS  K +K K + FFVG+P+PD EA+E+W WRY+ K  +
Sbjct:  356 KLDCTLITLSSSDEQFSSIIMPSSEKSDLDGDCFRQSPITATSLFGANNGKRERTFIELPYSDEECSPKKFKSMDSSVDKWNKYKHMPFFVGDPIPDDEAQERWRWRYERKSQR 469    
BLAST of DCAR_028247 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5C7HAG9 ((Uncharacterized protein {ECO:0000313|EMBL:TXG54243.1}))

HSP 1 Score: 870.537 bits (2248), Expect = 0.000e+0
Identity = 425/682 (62.32%), Postives = 508/682 (74.49%), Query Frame = 0
 
Query:  242 REKWNWRYQLKVMKDMASFHDKKRLFYSTLENDNPLDCLISKVNVIEITREPGFQLNANQSTCFYYDMEYCIEYSTFRNLLTDNSVMRHAISSLNNTDTFRTNASASTPSLEEDLSNCRSQNPDFALLDLYAGCGGMSTGLCLGAELSGIKLVTKWAVDYQKSACDSLILNHPETQVRNMPAEDFLGLLKEWEKLCNLYAL-------AFDDSDTIESKISTDGLEDVEVPRGEYEVSSFVDITYGTISKSGKQGLKFK---------------------------DFVRSGSKSKILPVPGDVDVICGGPPCQGISGYNRFRNVDAPLTDERNHQIVVFMDIVKFLKPKYVLMENVTDILKFDKGSLGRYALSRMVDMKYQARLGIMPAGCYGLPQFRLRVFLWGALPSEKLPQFPLPTHDVVVKFWPPPEFEQNTVAYNEGQPRDLEEAAILRDAISDLPAVTSQETREEMSYEMHPETEFQKYIRLSKYEITGYRSNIPESETPVLYDHRSYQLSEDDYLRICLIPRRKGANFRDLPGVVVDQDNKVCRDPTKELEMLPSGKPLVPDYVFTFEKGK---PFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKFPY 886
            R +W ++ +  VMK+ A FHD +RLFYST+ NDNP+DC++SKV++ +I  + G + N+  S+ FY+DMEYC+EYSTFR L +D S+  H +S         T A A +    E++ NC +   +  LLDLY+GCGGMSTGLCLGA+LS I LVTKWAVD  KSAC+SL LNHPET+V N  AEDFL LLKEW+KLCN Y +           + +I  + +    +  + PRGEYEV S VD+ YG  +K+GK+ L FK                           DFVR G KSKILP+PGDVDVICGGPPCQGISGYNR+RNVD+PL DERN QIV+FM+IV+FLKP +VLMENV DIL+FDK SLGRYALSR+V MKYQARLGI+ AGCYGLPQFRLRVFLWGALP+ KLPQFPLPTHDV+V++WPPPEFE+NTVAY+E QPRDLE+A +L+DAISDLP VTS E REEMSY   PETEFQ+++R S+ E+TG   N        LYDHR Y LSEDDY+R+C IP+RKGANFRDLPGVVV  DN   RDPTKE  +LPSGKP+VPDY  +FE+GK   PFARLWWDETV T+VTFP+ H+MA LHPEQDRVLT+RE ARLQGFPDYYRFCGTVK RYCQVGNAV V V+R LGYALG+A+QK   D PL+ LPPKF +
Sbjct:  382 RVQWFYKAEDTVMKEAAEFHDSRRLFYSTVMNDNPVDCIVSKVSITQIPPKIGLKSNSIPSSGFYFDMEYCVEYSTFRTLPSDKSLKIHELSLPRCLKNVTTAAVAHS---LENMPNCETCKAELVLLDLYSGCGGMSTGLCLGAKLSSINLVTKWAVDSDKSACESLRLNHPETRVWNEKAEDFLQLLKEWQKLCNRYEVNNVEREHQLRSAASIVGRTNVTSTKGADAPRGEYEVGSLVDVCYGDPNKTGKRRLNFKVHWKGYSSSEDTWEPIEGLSNCQERIQDFVRKGYKSKILPLPGDVDVICGGPPCQGISGYNRYRNVDSPLDDERNRQIVIFMNIVEFLKPMFVLMENVVDILRFDKASLGRYALSRLVHMKYQARLGIIAAGCYGLPQFRLRVFLWGALPNVKLPQFPLPTHDVIVRYWPPPEFERNTVAYDEDQPRDLEKALVLQDAISDLPIVTSHEAREEMSYSKPPETEFQRFMRSSESEVTGSAFNGTVGANSKLYDHRPYPLSEDDYIRVCQIPKRKGANFRDLPGVVVGIDNVARRDPTKEQTLLPSGKPMVPDYALSFEQGKSKRPFARLWWDETVPTIVTFPALHSMAVLHPEQDRVLTIRECARLQGFPDYYRFCGTVKARYCQVGNAVPVFVARTLGYALGIAYQKLNGDEPLMTLPPKFSH 1060    

HSP 2 Score: 175.637 bits (444), Expect = 3.532e-42
Identity = 87/159 (54.72%), Postives = 105/159 (66.04%), Query Frame = 0
 
Query:  844 RYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKFPYLVPE--------RTEKPFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKFPY 994
            R CQ+            G  +G+      D      L P    +VP+        ++++PFARLWWDETV T+VTFP+ H+MA LHPEQDRVLT+RE ARLQGFPDYYRFCGTVK RYCQVGNAV V V+R LGYALG+A+QK   D PL+ LPPKF +
Sbjct:  902 RVCQIPKRKGANFRDLPGVVVGIDNVARRDPTKEQTLLPSGKPMVPDYALSFEQGKSKRPFARLWWDETVPTIVTFPALHSMAVLHPEQDRVLTIRECARLQGFPDYYRFCGTVKARYCQVGNAVPVFVARTLGYALGIAYQKLNGDEPLMTLPPKFSH 1060    

HSP 3 Score: 41.5874 bits (96), Expect = 1.114e+0
Identity = 22/56 (39.29%), Postives = 33/56 (58.93%), Query Frame = 0
 
Query:  199 TPVKVNNRPKAGNGKGSD-SSSSKAHKQK-SVAFFVGEPVPDVEAREKWNWRYQLK 252
            TP +    P A   K    +SS+K  K+K  V++ +G+P+   EA+E+W WRY LK
Sbjct:  112 TPKRPRRSPHAPTIKMETLTSSNKIQKKKVHVSYLIGDPIHAEEAQERWRWRYDLK 167    
BLAST of DCAR_028247 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P9EH73 ((DNA (Cytosine-5)-methyltransferase CMT2-like isoform X3 {ECO:0000313|RefSeq:XP_035542187.1}))

HSP 1 Score: 869.766 bits (2246), Expect = 0.000e+0
Identity = 427/674 (63.35%), Postives = 508/674 (75.37%), Query Frame = 0
 
Query:  242 REKWNWRYQLKVMKDMASFHDKKRLFYSTLENDNPLDCLISKVNVIEITREPGFQLNANQSTCFYYDMEYCIEYSTFRNLLTDNSVMRHAISSLNNTDTFRTNASASTPSLEEDLSNCRSQNPDFALLDLYAGCGGMSTGLCLGAELSGIKLVTKWAVDYQKSACDSLILNHPETQVRNMPAEDFLGLLKEWEKLCNLYALAFDDSDTIESKISTDGLEDVE-VPRGEYEVSSFVDITYGTISKSGKQGLKFK---------------------------DFVRSGSKSKILPVPGDVDVICGGPPCQGISGYNRFRNVDAPLTDERNHQIVVFMDIVKFLKPKYVLMENVTDILKFDKGSLGRYALSRMVDMKYQARLGIMPAGCYGLPQFRLRVFLWGALPSEKLPQFPLPTHDVVVKFWPPPEFEQNTVAYNEGQPRDLEEAAILRDAISDLPAVTSQETREEMSYEMHPETEFQKYIRLSKYEITGYRSNIPESETPVLYDHRSYQLSEDDYLRICLIPRRKGANFRDLPGVVVDQDNKVCRDPTKELEMLPSGKPLVPDYVFTFEKGK---PFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKF 884
            R +W ++ +  V+K+  +FH+K+RLFYST+ NDN +DC+ISKV+V +IT   G  LN    + FY+DMEYC++YSTFR+L+ DNSV  H +SS +      T A   TP+  +++ N  +     ALLDL++GCGGMSTG+CLGA+L+G+ L  +WA+D   SAC+SL LNHPET VRN  A DFL LL EWEKLC  Y L      T  S++ T G +    +P GEYEVS  VDI YG  +K+GK+GL FK                           DFV++G KSKILP+PGDVDVICGGPPCQGISGYNR+RN+D+PL+D+RN QIVVFMDIV FLKPK+VLMENV DIL+FDK SLGRYALSR+V M YQARLG + AGCYGLPQFRLRVFLWGA PSEKLPQFPLPTHDV+V++WPPPEFE+NTVAY E QPR+LEEA +LRDAISDLPAVT+ E REEMS E  PET+FQ+YIR ++YE+TG   N        LYDHR Y LSEDDYLR+C IP+RKGANFRDLPGVVV  DN   RD T E ++LPSGK LVPDY FTFE+GK   PFARLWWDETV TVVT PSCHN A+LHPEQDRVLT+RE+ARLQGFPDYYR CGT+KERYCQVGNAVA+ V+RALGYALG+AF+K   D PL+ LPPKF
Sbjct:  519 RVQWFYKVEDTVIKEEGAFHNKRRLFYSTIMNDNLIDCIISKVSVTQITPRVGRILNFILPSDFYFDMEYCVDYSTFRSLVIDNSVRSHDVSSPDGIKPVDTRA---TPTFSQNVPNSETYEAKLALLDLFSGCGGMSTGMCLGAKLTGVNLAMRWALDSDGSACESLKLNHPETLVRNETAADFLELLMEWEKLCGHYRLN-GAGRTHPSRVMTSGADSCNGIPTGEYEVSRLVDICYGDPNKTGKRGLNFKVHWKGYSTSEDTWEPIEGLSNCQERIQDFVQNGLKSKILPLPGDVDVICGGPPCQGISGYNRYRNIDSPLSDDRNRQIVVFMDIVTFLKPKFVLMENVVDILRFDKASLGRYALSRLVHMNYQARLGTIAAGCYGLPQFRLRVFLWGAHPSEKLPQFPLPTHDVIVRYWPPPEFERNTVAYEEDQPRELEEALVLRDAISDLPAVTNFEIREEMSREKPPETDFQRYIRSTRYEMTGSALNDSLETKHSLYDHRPYALSEDDYLRVCQIPKRKGANFRDLPGVVVGADNVARRDRT-EQKLLPSGKSLVPDYAFTFEQGKSRRPFARLWWDETVPTVVTSPSCHNQAALHPEQDRVLTIREYARLQGFPDYYRLCGTIKERYCQVGNAVAIPVARALGYALGLAFRKLSRDEPLMTLPPKF 1187    

HSP 2 Score: 184.882 bits (468), Expect = 6.647e-45
Identity = 92/157 (58.60%), Postives = 108/157 (68.79%), Query Frame = 0
 
Query:  844 RYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKFPYLVPE--------RTEKPFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKF 992
            R CQ+            G  +G A   +  D    KL P    LVP+        ++ +PFARLWWDETV TVVT PSCHN A+LHPEQDRVLT+RE+ARLQGFPDYYR CGT+KERYCQVGNAVA+ V+RALGYALG+AF+K   D PL+ LPPKF
Sbjct: 1032 RVCQIPKRKGANFRDLPGVVVG-ADNVARRDRTEQKLLPSGKSLVPDYAFTFEQGKSRRPFARLWWDETVPTVVTSPSCHNQAALHPEQDRVLTIREYARLQGFPDYYRLCGTIKERYCQVGNAVAIPVARALGYALGLAFRKLSRDEPLMTLPPKF 1187    

HSP 3 Score: 52.373 bits (124), Expect = 4.967e-4
Identity = 34/114 (29.82%), Postives = 51/114 (44.74%), Query Frame = 0
 
Query:  169 KVAVSTVGLKRDDEDESGLIEFGDDQEG------RQTPVKVNNRPKAGNGK---------------------GSDSSSSKAHKQKSVAFFVGEPVPDVEAREKWNWRYQLKVMK 255
            K+  + + L   DE  S +I    ++        RQ+P+   +   A NGK                       DSS  K +K K + FFVG+P+PD EA+E+W WRY+ K  +
Sbjct:  338 KLDCTLITLSSSDEQFSSIIMPSSEKSDLDGDCFRQSPITATSLFGANNGKRERTFIELPYSDEECSPKKFKSMDSSVDKWNKYKHMPFFVGDPIPDDEAQERWRWRYERKSQR 451    
BLAST of DCAR_028247 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2I4DHF4 ((DNA (Cytosine-5)-methyltransferase CMT2-like isoform X1 {ECO:0000313|RefSeq:XP_018806586.1}))

HSP 1 Score: 869.766 bits (2246), Expect = 0.000e+0
Identity = 427/674 (63.35%), Postives = 508/674 (75.37%), Query Frame = 0
 
Query:  242 REKWNWRYQLKVMKDMASFHDKKRLFYSTLENDNPLDCLISKVNVIEITREPGFQLNANQSTCFYYDMEYCIEYSTFRNLLTDNSVMRHAISSLNNTDTFRTNASASTPSLEEDLSNCRSQNPDFALLDLYAGCGGMSTGLCLGAELSGIKLVTKWAVDYQKSACDSLILNHPETQVRNMPAEDFLGLLKEWEKLCNLYALAFDDSDTIESKISTDGLEDVE-VPRGEYEVSSFVDITYGTISKSGKQGLKFK---------------------------DFVRSGSKSKILPVPGDVDVICGGPPCQGISGYNRFRNVDAPLTDERNHQIVVFMDIVKFLKPKYVLMENVTDILKFDKGSLGRYALSRMVDMKYQARLGIMPAGCYGLPQFRLRVFLWGALPSEKLPQFPLPTHDVVVKFWPPPEFEQNTVAYNEGQPRDLEEAAILRDAISDLPAVTSQETREEMSYEMHPETEFQKYIRLSKYEITGYRSNIPESETPVLYDHRSYQLSEDDYLRICLIPRRKGANFRDLPGVVVDQDNKVCRDPTKELEMLPSGKPLVPDYVFTFEKGK---PFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKF 884
            R +W ++ +  V+K+  +FH+K+RLFYST+ NDN +DC+ISKV+V +IT   G  LN    + FY+DMEYC++YSTFR+L+ DNSV  H +SS +      T A   TP+  +++ N  +     ALLDL++GCGGMSTG+CLGA+L+G+ L  +WA+D   SAC+SL LNHPET VRN  A DFL LL EWEKLC  Y L      T  S++ T G +    +P GEYEVS  VDI YG  +K+GK+GL FK                           DFV++G KSKILP+PGDVDVICGGPPCQGISGYNR+RN+D+PL+D+RN QIVVFMDIV FLKPK+VLMENV DIL+FDK SLGRYALSR+V M YQARLG + AGCYGLPQFRLRVFLWGA PSEKLPQFPLPTHDV+V++WPPPEFE+NTVAY E QPR+LEEA +LRDAISDLPAVT+ E REEMS E  PET+FQ+YIR ++YE+TG   N        LYDHR Y LSEDDYLR+C IP+RKGANFRDLPGVVV  DN   RD T E ++LPSGK LVPDY FTFE+GK   PFARLWWDETV TVVT PSCHN A+LHPEQDRVLT+RE+ARLQGFPDYYR CGT+KERYCQVGNAVA+ V+RALGYALG+AF+K   D PL+ LPPKF
Sbjct:  537 RVQWFYKVEDTVIKEEGAFHNKRRLFYSTIMNDNLIDCIISKVSVTQITPRVGRILNFILPSDFYFDMEYCVDYSTFRSLVIDNSVRSHDVSSPDGIKPVDTRA---TPTFSQNVPNSETYEAKLALLDLFSGCGGMSTGMCLGAKLTGVNLAMRWALDSDGSACESLKLNHPETLVRNETAADFLELLMEWEKLCGHYRLN-GAGRTHPSRVMTSGADSCNGIPTGEYEVSRLVDICYGDPNKTGKRGLNFKVHWKGYSTSEDTWEPIEGLSNCQERIQDFVQNGLKSKILPLPGDVDVICGGPPCQGISGYNRYRNIDSPLSDDRNRQIVVFMDIVTFLKPKFVLMENVVDILRFDKASLGRYALSRLVHMNYQARLGTIAAGCYGLPQFRLRVFLWGAHPSEKLPQFPLPTHDVIVRYWPPPEFERNTVAYEEDQPRELEEALVLRDAISDLPAVTNFEIREEMSREKPPETDFQRYIRSTRYEMTGSALNDSLETKHSLYDHRPYALSEDDYLRVCQIPKRKGANFRDLPGVVVGADNVARRDRT-EQKLLPSGKSLVPDYAFTFEQGKSRRPFARLWWDETVPTVVTSPSCHNQAALHPEQDRVLTIREYARLQGFPDYYRLCGTIKERYCQVGNAVAIPVARALGYALGLAFRKLSRDEPLMTLPPKF 1205    

HSP 2 Score: 184.882 bits (468), Expect = 6.403e-45
Identity = 92/157 (58.60%), Postives = 108/157 (68.79%), Query Frame = 0
 
Query:  844 RYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKFPYLVPE--------RTEKPFARLWWDETVGTVVTFPSCHNMASLHPEQDRVLTLREFARLQGFPDYYRFCGTVKERYCQVGNAVAVTVSRALGYALGMAFQKSGDDGPLLKLPPKF 992
            R CQ+            G  +G A   +  D    KL P    LVP+        ++ +PFARLWWDETV TVVT PSCHN A+LHPEQDRVLT+RE+ARLQGFPDYYR CGT+KERYCQVGNAVA+ V+RALGYALG+AF+K   D PL+ LPPKF
Sbjct: 1050 RVCQIPKRKGANFRDLPGVVVG-ADNVARRDRTEQKLLPSGKSLVPDYAFTFEQGKSRRPFARLWWDETVPTVVTSPSCHNQAALHPEQDRVLTIREYARLQGFPDYYRLCGTIKERYCQVGNAVAIPVARALGYALGLAFRKLSRDEPLMTLPPKF 1205    

HSP 3 Score: 52.373 bits (124), Expect = 5.331e-4
Identity = 36/114 (31.58%), Postives = 52/114 (45.61%), Query Frame = 0
 
Query:  169 KVAVSTVGLKRDDEDESGLI---EFGDDQEG---RQTPVKVNNRPKAGNGK---------------------GSDSSSSKAHKQKSVAFFVGEPVPDVEAREKWNWRYQLKVMK 255
            K+  + + L   DE  S +I       D +G   RQ+P+   +   A NGK                       DSS  K +K K + FFVG+P+PD EA+E+W WRY+ K  +
Sbjct:  356 KLDCTLITLSSSDEQFSSIIMPSSEKSDLDGDCFRQSPITATSLFGANNGKRERTFIELPYSDEECSPKKFKSMDSSVDKWNKYKHMPFFVGDPIPDDEAQERWRWRYERKSQR 469    
Match NameStatsDescription
A0A5J5A207E-Value: 9.234e-46, PID: 67.83(Uncharacterized protein {ECO:0000313|EMBL:KAA8525... [more]
A0A2R6Q374E-Value: 1.258e-43, PID: 65.10DNA (cytosine-5-)-methyltransferase {ECO:0000256|A... [more]
A0A4S4E0R5E-Value: 5.518e-43, PID: 65.07Cytosine-specific methyltransferase {ECO:0000256|R... [more]
A0A5N6R441E-Value: 1.087e-44, PID: 63.69Cytosine-specific methyltransferase {ECO:0000256|R... [more]
A0A251P2J6E-Value: 1.171e-41, PID: 56.29Cytosine-specific methyltransferase {ECO:0000256|R... [more]
A0A7J0GCT9E-Value: 3.197e-44, PID: 64.44Cytosine-specific methyltransferase {ECO:0000256|R... [more]
A0A6P9E470E-Value: 6.034e-45, PID: 63.35(DNA (Cytosine-5)-methyltransferase CMT2-like isof... [more]
A0A5C7HAG9E-Value: 3.532e-42, PID: 62.32(Uncharacterized protein {ECO:0000313|EMBL:TXG5424... [more]
A0A6P9EH73E-Value: 6.647e-45, PID: 63.35(DNA (Cytosine-5)-methyltransferase CMT2-like isof... [more]
A0A2I4DHF4E-Value: 6.403e-45, PID: 63.35(DNA (Cytosine-5)-methyltransferase CMT2-like isof... [more]

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Analysis: 
NameDescription

An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4.

There are a few different naming schemes for this assembly. First there is the
Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below.
These sequences can be viewed in JBrowse here.

Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0

LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926.

Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence.

RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records.

The NCBI Sequence report lists the correspondences between the various naming methods

Link to the LNRQ01000000.1 master record at NCBI

Raw Reads: Link to SRA accessions used for the genome assembly

This genome is available in the CarrotOmics Blast Search

This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions.

For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins.

Data from this analysis can be viewed in JBrowse here.

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