DCAR_026720

Resource Type: 
Polypeptide
Name: 
DCAR_026720
Identifier: 
DCAR_026720.mRNA-protein
Sequence: 
MAEKQLIVVVEGTAAMGPHWQTILTEYLDKVIKSFCGYDSTTQKPSVPVE
FSLVVFNAPGNYSACLVQRSGWTRDVDNFFQWLSAISFSGGGFSDVAISE
GLAEALMMCTPLNGKQPHQNVDKQRHCILVAASNPFPLPTPVFRPQIPKL
EQSENTESQKESRISDAETLAKFFPQLSVSLSVISPKQLPKLKAIYNAGK
RNPRAADPPIDNVKSPYHLVLISENFIEARAALSRNGIAPLVSNHSPMKV
DTTSVMKVDTSPVLPVSVSPPTSVPSANGSVMNRQPIIVGNVTPTIVKIE
PTTVTSMVSGPAYPHVSSVPRAASQGVPTLQTSSPLSAPQEMIPTTETVP
DLKPPVTGLSQPLRPLGPAAANVSILNNLSQARQVMNSAALAGGTSIGLQ
SMGGTHMAMHMSNMISSGMVSSVPPTQTVLSSGQSGMTSIAASGQPGMTS
MAGTGALAGNAQVALNTNSGSFASASSNLSGNSNLGMSQPMGNTQGGVNM
GQSVPVVSQANISSTQMAQNGIGMNTNMISGVSQSGISSANGTMMPTPGM
GPQGQPGPQSLGMANNAAANMPIPSQASNAMQSAPSQSKYVRVWEGNLSG
QRQGQPVFITRLEGYRSASASETLAANWPPTMQIVRLISQDHMNNKQYVG
KADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGML
FPGGRQMASQGQVTSQGPPSMPGGGSYLS*
Sequence Length: 
730
Sequence Checksum: 
d8d1e93d82135a35ffc4ec206e869319
View location in JBrowse: 
Relationship: 
There is 1 relationship.
Relationships
The polypeptide, DCAR_026720, derives from mRNA, DCAR_026720.
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Blast Results: 
The following BLAST results are available for this feature:
BLAST of DCAR_026720 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
BLAST of DCAR_026720 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5J5ANN6 (Mediator of RNA polymerase II transcription subunit 25 {ECO:0000256|ARBA:ARBA00019694})

HSP 1 Score: 994.186 bits (2569), Expect = 0.000e+0
Identity = 510/707 (72.14%), Postives = 577/707 (81.61%), Query Frame = 0
 
Query:    1 MAEKQLIVVVEGTAAMGPHWQTILTEYLDKVIKSFCGYDSTTQKPSVP-VEFSLVVFNAPGNYSACLVQRSGWTRDVDNFFQWLSAISFSGGGFSDVAISEGLAEALMMCTPLNGKQPHQNVDKQRHCILVAASNPFPLPTPVFRPQIPKLEQSENTESQKESRISDAETLAKFFPQLSVSLSVISPKQLPKLKAIYNAGKRNPRAADPPIDNVKSPYHLVLISENFIEARAALSRNGIAPLVSNHSPMKVDTTSVMKVDTSPVLPVSVSPPTSVPSANGSVMNRQPI---IVGNVTPTIVKIEPTTVTSMVSGPAYPHVSSVPRAASQGVPTLQTSSPLSAPQEMIPTTETVPDLKPPVTGLSQPLRPLGPAAANVSILNNLSQARQVMNSAALAGGTSIGLQSMGGTHMAMHMSNMISSGMVSSVPPTQTVLSSGQSGMTSIAASGQPGMTSMAGTGALAGNAQVALNTNSGSFASASSNLSGNSNLGMSQPMGNTQGGVNMGQSVPVVSQANISSTQMAQNGIGMNTNMISGVSQSGISSANGTMMPTPGMGPQGQPGPQSLGMANNAAANMPIPSQASNAMQSAPSQSKYVRVWEGNLSGQRQGQPVFITRLEGYRSASASETLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPG 703
            MA+KQL+V VEGTAA+GP WQ+I  +YL  ++++FCG +   QKPS   +E SLVVFNA G+YSACLVQRSGWT+DVD F QWLSA+ F+GGGF+D AI+EGLAEALMM + +NG Q   NVD QRHCILVAASNP+PLPTPV+RPQ+  LEQ EN E+Q ES +SDAE++AK F Q SVSLSVI PKQLPKL+AIY+AGKRN RAADPP+DNVK+P+ LVLISENF+EARAALSR G+    SN SP+K+D  SV      P   VS  P TS+PS NGS+MNRQPI    VGN+TP  VK+EP+TVTSM SGPA+PH+SSVPRA SQGVP+LQTSSP+SA QEMI   + V DLKP V+G+SQ LRP GPAAANVSILN+LSQARQVMNSAAL GGTSIGL SMGG  MAMHMSNMISSGM SSVP  QTVLSSGQSG+TS+           AG+G LAG  QVA N+  GSF SAS+N+S NSN G+SQPMGN QGG +MGQSV  + Q NI  TQM Q+GIGMN NMISG+  SG+SS  GTM+PTPGM  Q Q G  SLG+ NN+AANM +P Q S+A+QSA  QSKYV+VWEGNLSGQRQGQPVFITRLEGYRSASASETLAANWP TMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPG
Sbjct:    1 MAKKQLVVAVEGTAALGPFWQSISGDYLVDIVRTFCGNELAGQKPSAANIELSLVVFNAHGSYSACLVQRSGWTKDVDIFLQWLSALPFAGGGFNDAAIAEGLAEALMMFSTINGSQTQLNVDGQRHCILVAASNPYPLPTPVYRPQMQNLEQGENIETQSESCLSDAESVAKSFSQCSVSLSVICPKQLPKLRAIYSAGKRNARAADPPVDNVKNPHFLVLISENFMEARAALSRPGVTNFASNPSPVKMDIASV------PT--VSGPPSTSIPSVNGSIMNRQPISVGTVGNITPATVKVEPSTVTSMPSGPAFPHISSVPRATSQGVPSLQTSSPISASQEMISNNDNVQDLKPTVSGMSQSLRPGGPAAANVSILNSLSQARQVMNSAALTGGTSIGLSSMGGASMAMHMSNMISSGMASSVPAAQTVLSSGQSGITSV-----------AGSGTLAGTVQVAQNSAPGSFTSASNNVSANSNPGISQPMGNLQGGASMGQSVSAMGQGNIPGTQMVQSGIGMNPNMISGLGPSGMSSGTGTMIPTPGMTQQVQGGMPSLGVNNNSAANMSLPQQTSSALQSA--QSKYVKVWEGNLSGQRQGQPVFITRLEGYRSASASETLAANWPQTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPG 686    
BLAST of DCAR_026720 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B7ATQ3 (Mediator of RNA polymerase II transcription subunit 25 {ECO:0000256|ARBA:ARBA00019694})

HSP 1 Score: 986.867 bits (2550), Expect = 0.000e+0
Identity = 510/707 (72.14%), Postives = 576/707 (81.47%), Query Frame = 0
 
Query:    1 MAEKQLIVVVEGTAAMGPHWQTILTEYLDKVIKSFCGYDSTTQKPSVP-VEFSLVVFNAPGNYSACLVQRSGWTRDVDNFFQWLSAISFSGGGFSDVAISEGLAEALMMCTPLNGKQPHQNVDKQRHCILVAASNPFPLPTPVFRPQIPKLEQSENTESQKESRISDAETLAKFFPQLSVSLSVISPKQLPKLKAIYNAGKRNPRAADPPIDNVKSPYHLVLISENFIEARAALSRNGIAPLVSNHSPMKVDTTSVMKVDTSPVLPVSVSPPTSVPSANGSVMNRQPI---IVGNVTPTIVKIEPTTVTSMVSGPAYPHVSSVPRAASQGVPTLQTSSPLSAPQEMIPTTETVPDLKPPVTGLSQPLRPLGPAAANVSILNNLSQARQVMNSAALAGGTSIGLQSMGGTHMAMHMSNMISSGMVSSVPPTQTVLSSGQSGMTSIAASGQPGMTSMAGTGALAGNAQVALNTNSGSFASASSNLSGNSNLGMSQPMGNTQGGVNMGQSVPVVSQANISSTQMAQNGIGMNTNMISGVSQSGISSANGTMMPTPGMGPQGQPGPQSLGMANNAAANMPIPSQASNAMQSAPSQSKYVRVWEGNLSGQRQGQPVFITRLEGYRSASASETLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPG 703
            MA+KQLIV VEGTAA+GP W +I  +YL  ++++FCG + T QKPS P +E SLV+FNA G+YSACLVQ+SGWT+DVD F QWLSAI F+GGGF+D AI+EGLAEALMM + +NG Q   NVD QRHCILVAASNP+PLPTPV+RPQ+  LEQSEN E+Q ES + DAET+AK F Q SVSLSVI PKQLPKLKAIYNAGKRN R ADPP+DNVK+P+ LVLISENF+EARAALSR G+    SN SP+K+D  +        V PVS  P TS+ S NGS+MNRQPI    VGN+TP  VK+EP+TVTSMVSGPA+PH+ SVPRA SQGVP+LQTSSP+SA QEMI   + V DLKP V+G+SQPLRP GPAAANVSILN+LSQARQ     AL+GGTSIGL SMGGT MAMHMSNMISSGMVSSVP  QTVLSSGQSG+TS+           AG+G LAG  QVA N+  GSF SA+S+L GN N GMSQPMGN QGGV+MGQSV  +SQ N+  TQM Q+GIGMN NMISG+  SG+SS  GTM+PTPGM  Q Q G  SLG+ NN+AANM +P Q S+A+QSA  QSKYV+VWEGNLSGQRQGQPVFITRLEGYRSASASETLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKA RLIGMLFPG
Sbjct:    1 MAKKQLIVAVEGTAALGPFWHSISADYLGDIVRTFCGNELTGQKPSAPNIELSLVMFNAHGSYSACLVQQSGWTKDVDIFLQWLSAIPFAGGGFNDAAIAEGLAEALMMFSTINGSQTQLNVDGQRHCILVAASNPYPLPTPVYRPQMQNLEQSENIETQTESCLYDAETVAKSFAQSSVSLSVICPKQLPKLKAIYNAGKRNTRVADPPVDNVKNPHFLVLISENFMEARAALSRPGVTSFASNQSPVKMDIAT--------VPPVSGPPSTSITSVNGSIMNRQPISVGTVGNMTPATVKVEPSTVTSMVSGPAFPHIPSVPRATSQGVPSLQTSSPISASQEMISNNDNVQDLKPTVSGMSQPLRPGGPAAANVSILNSLSQARQ-----ALSGGTSIGLPSMGGTSMAMHMSNMISSGMVSSVPAAQTVLSSGQSGITSV-----------AGSGTLAGTVQVAQNSAPGSFTSATSSLPGNPNPGMSQPMGNLQGGVSMGQSVSGMSQGNLPGTQMVQSGIGMNPNMISGLGPSGMSSGTGTMIPTPGMSQQVQQGMPSLGVNNNSAANMSLPQQTSSALQSA--QSKYVKVWEGNLSGQRQGQPVFITRLEGYRSASASETLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKAYRLIGMLFPG 681    

HSP 2 Score: 39.2762 bits (90), Expect = 3.039e+0
Identity = 18/24 (75.00%), Postives = 20/24 (83.33%), Query Frame = 0
 
Query:  702 PGGRQMASQGQVTSQGPPSMPGGG 725
            P   Q+ SQGQV+SQGPPSMPGGG
Sbjct:  787 PSRSQLVSQGQVSSQGPPSMPGGG 810    
BLAST of DCAR_026720 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B7AVZ0 (Mediator of RNA polymerase II transcription subunit 25 {ECO:0000256|ARBA:ARBA00019694})

HSP 1 Score: 986.097 bits (2548), Expect = 0.000e+0
Identity = 510/707 (72.14%), Postives = 576/707 (81.47%), Query Frame = 0
 
Query:    1 MAEKQLIVVVEGTAAMGPHWQTILTEYLDKVIKSFCGYDSTTQKPSVP-VEFSLVVFNAPGNYSACLVQRSGWTRDVDNFFQWLSAISFSGGGFSDVAISEGLAEALMMCTPLNGKQPHQNVDKQRHCILVAASNPFPLPTPVFRPQIPKLEQSENTESQKESRISDAETLAKFFPQLSVSLSVISPKQLPKLKAIYNAGKRNPRAADPPIDNVKSPYHLVLISENFIEARAALSRNGIAPLVSNHSPMKVDTTSVMKVDTSPVLPVSVSPPTSVPSANGSVMNRQPI---IVGNVTPTIVKIEPTTVTSMVSGPAYPHVSSVPRAASQGVPTLQTSSPLSAPQEMIPTTETVPDLKPPVTGLSQPLRPLGPAAANVSILNNLSQARQVMNSAALAGGTSIGLQSMGGTHMAMHMSNMISSGMVSSVPPTQTVLSSGQSGMTSIAASGQPGMTSMAGTGALAGNAQVALNTNSGSFASASSNLSGNSNLGMSQPMGNTQGGVNMGQSVPVVSQANISSTQMAQNGIGMNTNMISGVSQSGISSANGTMMPTPGMGPQGQPGPQSLGMANNAAANMPIPSQASNAMQSAPSQSKYVRVWEGNLSGQRQGQPVFITRLEGYRSASASETLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPG 703
            MA+KQLIV VEGTAA+GP W +I  +YL  ++++FCG + T QKPS P +E SLV+FNA G+YSACLVQ+SGWT+DVD F QWLSAI F+GGGF+D AI+EGLAEALMM + +NG Q   NVD QRHCILVAASNP+PLPTPV+RPQ+  LEQSEN E+Q ES + DAET+AK F Q SVSLSVI PKQLPKLKAIYNAGKRN R ADPP+DNVK+P+ LVLISENF+EARAALSR G+    SN SP+K+D  +        V PVS  P TS+ S NGS+MNRQPI    VGN+TP  VK+EP+TVTSMVSGPA+PH+ SVPRA SQGVP+LQTSSP+SA QEMI   + V DLKP V+G+SQPLRP GPAAANVSILN+LSQARQ     AL+GGTSIGL SMGGT MAMHMSNMISSGMVSSVP  QTVLSSGQSG+TS+           AG+G LAG  QVA N+  GSF SA+S+L GN N GMSQPMGN QGGV+MGQSV  +SQ N+  TQM Q+GIGMN NMISG+  SG+SS  GTM+PTPGM  Q Q G  SLG+ NN+AANM +P Q S+A+QSA  QSKYV+VWEGNLSGQRQGQPVFITRLEGYRSASASETLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKA RLIGMLFPG
Sbjct:    1 MAKKQLIVAVEGTAALGPFWHSISADYLGDIVRTFCGNELTGQKPSAPNIELSLVMFNAHGSYSACLVQQSGWTKDVDIFLQWLSAIPFAGGGFNDAAIAEGLAEALMMFSTINGSQTQLNVDGQRHCILVAASNPYPLPTPVYRPQMQNLEQSENIETQTESCLYDAETVAKSFAQSSVSLSVICPKQLPKLKAIYNAGKRNTRVADPPVDNVKNPHFLVLISENFMEARAALSRPGVTSFASNQSPVKMDIAT--------VPPVSGPPSTSITSVNGSIMNRQPISVGTVGNMTPATVKVEPSTVTSMVSGPAFPHIPSVPRATSQGVPSLQTSSPISASQEMISNNDNVQDLKPTVSGMSQPLRPGGPAAANVSILNSLSQARQ-----ALSGGTSIGLPSMGGTSMAMHMSNMISSGMVSSVPAAQTVLSSGQSGITSV-----------AGSGTLAGTVQVAQNSAPGSFTSATSSLPGNPNPGMSQPMGNLQGGVSMGQSVSGMSQGNLPGTQMVQSGIGMNPNMISGLGPSGMSSGTGTMIPTPGMSQQVQQGMPSLGVNNNSAANMSLPQQTSSALQSA--QSKYVKVWEGNLSGQRQGQPVFITRLEGYRSASASETLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKAYRLIGMLFPG 681    

HSP 2 Score: 39.2762 bits (90), Expect = 3.127e+0
Identity = 18/24 (75.00%), Postives = 20/24 (83.33%), Query Frame = 0
 
Query:  702 PGGRQMASQGQVTSQGPPSMPGGG 725
            P   Q+ SQGQV+SQGPPSMPGGG
Sbjct:  766 PSRSQLVSQGQVSSQGPPSMPGGG 789    
BLAST of DCAR_026720 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2R6PH51 (Mediator of RNA polymerase II transcription subunit 25 {ECO:0000256|ARBA:ARBA00019694})

HSP 1 Score: 962.214 bits (2486), Expect = 0.000e+0
Identity = 494/706 (69.97%), Postives = 574/706 (81.30%), Query Frame = 0
 
Query:    1 MAEKQLIVVVEGTAAMGPHWQTILTEYLDKVIKSFCGYDSTTQKPSVP-VEFSLVVFNAPGNYSACLVQRSGWTRDVDNFFQWLSAISFSGGGFSDVAISEGLAEALMMCTPLNGKQPHQNVDKQRHCILVAASNPFPLPTPVFRPQIPKLEQSENTESQKESRISDAETLAKFFPQLSVSLSVISPKQLPKLKAIYNAGKRNPRAADPPIDNVKSPYHLVLISENFIEARAALSRNGIAPLVSNHSPMKVDTTSVMKVDTSPVLPVSVSPPTSVPSANGSVMNRQPIIVGNVTPTIVKIEPTTVTSMVSGPAYPHVSSVPRAASQGVPTLQTSSPLSAPQEMIPTTETVPDLKPPVTGLSQPLRPLGPAAANVSILNNLSQARQVMNSAALAGGTSIGLQSMGGTHMAMHMSNMISSGMVSSVPPTQTVLSSGQSGMTSIAASGQPGMTSMAGTGALAGNAQVALNTNSGSFASASSNLSGNSNLGMSQPMGNTQGGVNMGQSVPVVSQANISSTQMAQNGIGMNTNMISGVSQSGISSANGTMMPTPGMGPQGQPGPQSLGMANNAAANMPIPS--QASNAMQSAPSQSKYVRVWEGNLSGQRQGQPVFITRLEGYRSASASETLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPG 703
            MA+KQLIV +EGTA+MGPHWQ+I+++YLDK+I++FC      QK SV  VEF+LV FNA G+YSACLVQRSGWTRDVD F QWL+AI F+GGGF+D AI+EGL EA+MM + ++G Q  QNV  QRHCILVAASNP+PLPTPV+RP +  LEQ EN E Q +SR+SDAET+AK F Q SVSLSVI PKQLPKL+AIYNAGKRNPRAADPP+D+VK+P+ LVLISE+F+EARA+LSR GI  L S+ SP+K+D  SV  V   P         TS+   NGS+ NRQPI VGN+ P  VK+EP+TV SMVSGP++PH+ +VPRAASQGVP+LQTSSP+S+ QEMI   E V DLKP V+ ++QPLRP+GPAAANV+ILNNLSQARQVMNSAAL GGTSIGLQSMGGT MAMHMSNMISSGM SSVP  QTV+SSGQS + S           M+G+G+L G AQ A N+  GSF S++SN+S  SNLG+SQ +GN Q GV+MGQS P ++Q N+   QM QNGI MN NM+SG+  SG+SS  GTMMPTPGM  Q QPG QSLG+ N++A N+P+    Q S+ +QSA  QSKYV+VWEGNLSGQRQGQPVFITRLEGYRSASASETLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPG
Sbjct:    1 MADKQLIVAIEGTASMGPHWQSIVSDYLDKIIRTFCSNKLAGQKSSVSNVEFALVAFNAHGSYSACLVQRSGWTRDVDIFLQWLAAIPFAGGGFNDAAIAEGLGEAIMMFSGVSGSQTQQNVAGQRHCILVAASNPYPLPTPVYRPPLQNLEQGENIEVQTQSRLSDAETVAKSFGQCSVSLSVICPKQLPKLRAIYNAGKRNPRAADPPVDSVKNPFFLVLISEDFMEARASLSRPGITGLASSQSPVKMDIASVPPVSGPPP--------TSILPVNGSITNRQPISVGNIPPATVKVEPSTVGSMVSGPSFPHIPAVPRAASQGVPSLQTSSPMSS-QEMISNNENVQDLKPMVSSMAQPLRPVGPAAANVNILNNLSQARQVMNSAALTGGTSIGLQSMGGTPMAMHMSNMISSGMASSVPAAQTVISSGQSAIPS-----------MSGSGSLTGTAQPAPNSAPGSFTSSASNMSATSNLGISQTLGNLQSGVSMGQSAPGMNQGNLPGAQMVQNGISMNQNMMSGLGPSGMSSGTGTMMPTPGMTQQVQPGMQSLGVNNSSATNIPMSQVQQTSSGLQSA--QSKYVKVWEGNLSGQRQGQPVFITRLEGYRSASASETLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPG 684    

HSP 2 Score: 40.817 bits (94), Expect = 1.204e+0
Identity = 18/24 (75.00%), Postives = 21/24 (87.50%), Query Frame = 0
 
Query:  702 PGGRQMASQGQVTSQGPPSMPGGG 725
            PG  Q+ SQGQV+SQGPP+MPGGG
Sbjct:  802 PGRSQLVSQGQVSSQGPPNMPGGG 825    
BLAST of DCAR_026720 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P6BIP3 (Mediator of RNA polymerase II transcription subunit 25 {ECO:0000256|ARBA:ARBA00019694})

HSP 1 Score: 952.584 bits (2461), Expect = 0.000e+0
Identity = 493/706 (69.83%), Postives = 572/706 (81.02%), Query Frame = 0
 
Query:    1 MAEKQLIVVVEGTAAMGPHWQTILTEYLDKVIKSFCGYDSTTQKPSVP-VEFSLVVFNAPGNYSACLVQRSGWTRDVDNFFQWLSAISFSGGGFSDVAISEGLAEALMMCTPL--NGKQPHQNVDKQRHCILVAASNPFPLPTPVFRPQIPKLEQSENTESQKESRISDAETLAKFFPQLSVSLSVISPKQLPKLKAIYNAGKRNPRAADPPIDNVKSPYHLVLISENFIEARAALSRNGIAPLVSNHSPMKVDTTSVMKVDTSPVLPVSVSPPTSVPSANGSVMNRQPIIVGNVTPTIVKIEPTTVTSMVSGPAYPHVSSVPRAASQGVPTLQTSSPLSAPQEMIPTTETVPDLKPPVTGLSQPLRPLGPAAANVSILNNLSQARQVMNSAALAGGTSIGLQSMGGTHMAMHMSNMISSGMVSSVPPTQTVLSSGQSGMTSIAASGQPGMTSMAGTGALAGNAQVALNTNSGSFASASSNLSGNSNLGMSQPMGNTQGGVNMGQSVPVVSQANISSTQMAQNGIGMNTNMISGVSQSGISSANGTMMPTPGMGPQGQPGPQSLGMANNAAANMPIPSQASNAMQSAPSQSKYVRVWEGNLSGQRQGQPVFITRLEGYRSASASETLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPG 703
            MA+KQLIV VEGTAAMGP+WQ ++++YLDK+I+ FC  + T QK S   VE SLV FN  G+Y ACLVQRSGWT+DVD F QWLSAI FSGGGF+D AI+EGL+EALMM  P+  NG Q  QNVD QRHCILVAASNP+PLPTPV+RPQI  LEQSEN E+Q ESR+SDAET+AK F Q SVSLSVI PK L KLKAIY+AGKRNPRA DPPIDNV++P +LVLISENF+E RAALSR+G+  L +N SP+K+D  SV  V T P        PT VPS NGS+M+RQP+ VGNV    VK+EPTT+TSM +GPA+ H+ SVPRA SQ VPTLQTSSP +  QE++ + + V +LKP V+G++QPLRP+ PAAANV+ILNNLSQAR VMNSAAL GGTSIGLQSMG + +AMHMSNMISSGM SSVPP QTV SSGQSGMTS           + G+GAL G AQV  N+   SF SA+SN++GNSN+G+SQPMGN QG V+MGQSVP +SQ N S  QM Q+G+GM+ NM SG+ QS +SS  G M+PTPGM  Q Q G Q+LG+++++AA+MP+  Q S+A+QSA  QSKYV+VWEGNLSGQRQGQPVFITRLEGYRSASASETLAA+WP TMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKA RLIGMLFPG
Sbjct:    1 MADKQLIVAVEGTAAMGPYWQIVVSDYLDKIIRCFCSGELTGQKNSTSNVELSLVTFNTHGSYCACLVQRSGWTKDVDIFLQWLSAIPFSGGGFNDAAIAEGLSEALMMF-PISPNGNQTQQNVDGQRHCILVAASNPYPLPTPVYRPQIQNLEQSENVEAQTESRLSDAETVAKSFAQCSVSLSVICPKLLSKLKAIYSAGKRNPRAPDPPIDNVRNPQYLVLISENFMEGRAALSRSGVPSLAANQSPVKMDMASVTSV-TGPA-------PTPVPSVNGSIMSRQPVSVGNVPTATVKVEPTTITSMATGPAFQHIPSVPRAPSQAVPTLQTSSPSTTTQEVMTSGDNVQELKPSVSGMTQPLRPVPPAAANVNILNNLSQAR-VMNSAALTGGTSIGLQSMGQSPVAMHMSNMISSGMASSVPPAQTVFSSGQSGMTS-----------LTGSGALTGTAQVPPNSGLSSFPSATSNVAGNSNIGISQPMGNVQGAVSMGQSVPGMSQGNHSGAQMVQSGVGMSQNM-SGLGQSAVSSGTGIMIPTPGMSQQVQSGMQTLGVSHSSAASMPLSQQTSSALQSA--QSKYVKVWEGNLSGQRQGQPVFITRLEGYRSASASETLAAHWPQTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKAYRLIGMLFPG 682    

HSP 2 Score: 40.0466 bits (92), Expect = 1.953e+0
Identity = 18/24 (75.00%), Postives = 20/24 (83.33%), Query Frame = 0
 
Query:  702 PGGRQMASQGQVTSQGPPSMPGGG 725
            PG  Q+ SQGQVTSQ PP+MPGGG
Sbjct:  865 PGRSQLVSQGQVTSQAPPNMPGGG 888    
BLAST of DCAR_026720 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A1R3J5Z7 (Mediator of RNA polymerase II transcription subunit 25 {ECO:0000256|ARBA:ARBA00019694})

HSP 1 Score: 947.192 bits (2447), Expect = 0.000e+0
Identity = 493/718 (68.66%), Postives = 573/718 (79.81%), Query Frame = 0
 
Query:    1 MAEKQLIVVVEGTAAMGPHWQTILTEYLDKVIKSFCGYDSTTQKPSVP-VEFSLVVFNAPGNYSACLVQRSGWTRDVDNFFQWLSAISFSGGGFSDVAISEGLAEALMMCT-PLNGKQPHQNVDKQRHCILVAASNPFPLPTPVFRPQIPKLEQSENTESQKESRISDAETLAKFFPQLSVSLSVISPKQLPKLKAIYNAGKRNPRAADPPIDNVKSPYHLVLISENFIEARAALSRNGIAPLVSNHSPMKVDTTSVMKVDTSPVLPVSVSPPTSVPSANGSVMNRQPIIVGNVTPTIVKIEPTTVTSMVSGPAYPHVSSVPRAASQGVPTLQTSSPLSAPQEMIPTTETVPDLKPPVTGLSQPLRPLGPAAANVSILNNLSQARQVMNSAALAGGTSIGLQSMGGTHMAMHMSNMISSGMVSSVPPTQTVLSSGQSGMTSIAASGQPGMTSMAGTGALAGNAQVALNTNSGSFASASSNLSGNSNLGMSQPMGNTQGGVNMGQSVPVVSQANISSTQMAQNGIGMNTNMISGVSQSGISSANGTMMPTPGMGPQGQPGPQSLGMANNAAANMPIPSQASNAMQSAPSQSKYVRVWEGNLSGQRQGQPVFITRLEGYRSASASET-------------LAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPG 703
            MAEKQLIV VEGTAAMGP+W T++++YLDK+I+ FC  +   QK S   VE SLV FN  G+Y ACLVQRSGWT+DVD F QWLSAI F+GGGF+D AI+EGL+EALMM +   NG Q  QNVD QRHCILV+ASNP+PLPTPV+RPQI  LEQ+EN E+Q ESR+SDAET+AK F Q S+SLSVI PKQL KLKAI++AGKRNPRA DPP+DN ++P  LVLISENF+E RAA SR G+  L SN SP+K+D  SV          V+V PPTSVPSANGS+M RQP+ VG+V    VK+EPTT+TSM +GPAYPH+ SVPRA SQ VPTLQTSSP +  QE+I + + V ++KP V+G++QPLRP+ P AANV+ILNNLSQAR VMNSAAL GG+SIGLQSMG T +AMHMSNMISSGM SSVPPTQTV S+GQS           GMTS+ G+GAL G AQV  N+   SFASASSN++GNSN+G+SQPMGN QGGV+MGQSVP +SQ N+S  QM Q+G+GM+ NM SG+  S +SS  GTM+PTPGM  Q Q   QSLG++N++AA+MP+  Q S+A+QSA  QSKYV+VWEGNLSGQRQGQPVFITRLEGYRSASASET             LAA+WP TMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPG
Sbjct:    1 MAEKQLIVAVEGTAAMGPYWHTVVSDYLDKIIRCFCSGELNGQKTSTSNVELSLVTFNTHGSYCACLVQRSGWTKDVDIFLQWLSAIPFAGGGFNDAAIAEGLSEALMMFSVASNGNQTQQNVDGQRHCILVSASNPYPLPTPVYRPQIQNLEQTENIEAQTESRLSDAETVAKSFGQCSISLSVICPKQLSKLKAIFSAGKRNPRAPDPPVDNSRNPQFLVLISENFMEGRAAFSRPGVPSLPSNQSPVKMDMASVTS--------VTVPPPTSVPSANGSMMARQPVSVGSVPTATVKVEPTTITSMGTGPAYPHIPSVPRAPSQAVPTLQTSSPSTTTQEVITSGDNVQEIKPSVSGMTQPLRPV-PPAANVNILNNLSQAR-VMNSAALTGGSSIGLQSMGQTPVAMHMSNMISSGMASSVPPTQTVFSNGQS-----------GMTSLTGSGALTGTAQVPPNSGLSSFASASSNMAGNSNIGISQPMGNVQGGVSMGQSVPGMSQGNVSGAQMVQSGVGMSQNM-SGLGPSTVSSGTGTMIPTPGMSQQVQSNMQSLGVSNSSAASMPLSQQTSSALQSA--QSKYVKVWEGNLSGQRQGQPVFITRLEGYRSASASETFIEPEDFGFCSFRLAAHWPQTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPG 694    

HSP 2 Score: 38.5058 bits (88), Expect = 5.619e+0
Identity = 17/24 (70.83%), Postives = 20/24 (83.33%), Query Frame = 0
 
Query:  702 PGGRQMASQGQVTSQGPPSMPGGG 725
            PG  Q+ SQGQV+SQ PP+MPGGG
Sbjct:  866 PGRSQLVSQGQVSSQAPPNMPGGG 889    
BLAST of DCAR_026720 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B6X4W5 (Mediator of RNA polymerase II transcription subunit 25 {ECO:0000256|ARBA:ARBA00019694})

HSP 1 Score: 946.421 bits (2445), Expect = 0.000e+0
Identity = 502/757 (66.31%), Postives = 587/757 (77.54%), Query Frame = 0
 
Query:    1 MAEKQLIVVVEGTAAMGPHWQTILTEYLDKVIKSFCGYDSTTQKPSVP-VEFSLVVFNAPGNYSACLVQRSGWTRDVDNFFQWLSAISFSGGGFSDVAISEGLAEALMMCTPL--NGKQPHQNVDKQRHCILVAASNPFPLPTPVFRPQIPKLEQSENTESQKESRISDAETLAKFFPQLSVSLSVISPKQLPKLKAIYNAGKRNPRAADPPIDNVKSPYHLVLISENFIEARAALSRNGIAPLVSNHSPMKVDTTSVMKVDTSPVLPVSVSPPTS---VPSANGSVMNRQPIIVGNVTPTIVKIEPTTVTSMVSGPAYPHVSSVPRAASQGVPTLQTSSPLSAPQEMIPTTETVPDLKPPVTGLSQPLRPLGPAAANVSILNNLSQARQVMNSAALAGGTSIGLQSMGGTHMAMHMSNMISSGMVSSVPPTQTVLSSGQSGMTSIAASGQPGMTSMAGTGALAGNAQVALNTNSGSFASASSNLSGNSNLGMSQPMGNTQGGVNMGQSVPVVSQANISSTQMAQNGIGMNTNMISGVSQSGISSANGTMMPTPGMGPQGQPGPQSLGMANNAAANMPIPSQASNAMQSAPSQSKYVRVWE----GNLSGQRQGQPVFITRLEGYRSASASETLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPG---------------------GR-QMASQGQVTSQGPPSMPGGG 725
            MAEKQLIV VEGTAAMGP+W  ++++YLDK+I+ FC  + T QK S   VEFSLV FN  G+Y ACLVQRSGWT+DVD F QWLSAI FSGGGF+D AI+EGL+EALMM  P+  NG Q  QNVD QRHCILVAASNP+PLPTPV+RPQI  LEQ E++E+Q ESR+SDAET+A+ F Q SVSLSVI PKQL KLKAIY+AGKRNPRAADPP+DNVK+   LVLISENF+E RAA SR+G+  L +N SP+K+D  SV  V T P       PP+S       NGS++ R P+ +GNV    VK+EPTT+TSM +GPA+ HV SVPRA SQ +PTLQTSSPL+  QE + + + + +LKP V+G++QP R + PAAANV+ILNNLSQAR VM+SAAL GGTSIGLQSMG T +AMHMSNMISSGM SSVPP  TVLSSGQ           P MTS+ G+GAL G AQV  N+   SF+SA+SN++GNSN+G+SQ MGN QG VN+GQSVP +SQ N S TQM Q+G+GM+ NM S + QS +SS NGTM+PTPGM  Q Q G Q+LG++N++AA+MP+  Q S+A+QSA  QSKYV+VWE    GNLSGQRQGQPVFITRLEGYRSASASETLAA+WP TMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLP QTLLLSVSDKACRLIGMLFPG                     GR Q+ SQGQV+SQ PP+MPGGG
Sbjct:    1 MAEKQLIVAVEGTAAMGPYWHIVVSDYLDKIIRCFCSSELTGQKNSTSNVEFSLVTFNTHGSYCACLVQRSGWTKDVDIFLQWLSAIPFSGGGFNDAAIAEGLSEALMMF-PIASNGNQTPQNVDGQRHCILVAASNPYPLPTPVYRPQIQNLEQVEHSEAQTESRLSDAETVARSFAQCSVSLSVICPKQLAKLKAIYSAGKRNPRAADPPVDNVKNSQFLVLISENFMEGRAAFSRSGVPSLATNQSPVKMDMASVTSV-TGP-------PPSSGGLCRILNGSMVGRPPVSIGNVATATVKVEPTTITSMATGPAFSHVPSVPRAPSQAIPTLQTSSPLTNTQEGMTSGDNMQELKPSVSGMTQPSRTVPPAAANVNILNNLSQAR-VMSSAALTGGTSIGLQSMGQTPVAMHMSNMISSGMASSVPPAHTVLSSGQ-----------PTMTSLTGSGALTGTAQVPPNSGLSSFSSATSNVAGNSNIGISQSMGNVQGAVNIGQSVPGMSQGNHSGTQMMQSGVGMSQNM-SALGQSTVSSGNGTMIPTPGMSQQVQSGMQTLGVSNSSAASMPLSQQTSSALQSA--QSKYVKVWEEEELGNLSGQRQGQPVFITRLEGYRSASASETLAAHWPQTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPQQTLLLSVSDKACRLIGMLFPGQLSQQQQMVGSGMGPAYVQGPGRSQLVSQGQVSSQAPPNMPGGG 733    
BLAST of DCAR_026720 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P4MG19 (Mediator of RNA polymerase II transcription subunit 25 {ECO:0000256|ARBA:ARBA00019694})

HSP 1 Score: 941.414 bits (2432), Expect = 0.000e+0
Identity = 489/706 (69.26%), Postives = 570/706 (80.74%), Query Frame = 0
 
Query:    1 MAEKQLIVVVEGTAAMGPHWQTILTEYLDKVIKSFCGYDSTTQKPSVP-VEFSLVVFNAPGNYSACLVQRSGWTRDVDNFFQWLSAISFSGGGFSDVAISEGLAEALMMCTPL--NGKQPHQNVDKQRHCILVAASNPFPLPTPVFRPQIPKLEQSENTESQKESRISDAETLAKFFPQLSVSLSVISPKQLPKLKAIYNAGKRNPRAADPPIDNVKSPYHLVLISENFIEARAALSRNGIAPLVSNHSPMKVDTTSVMKVDTSPVLPVSVSPPTSVPSANGSVMNRQPIIVGNVTPTIVKIEPTTVTSMVSGPAYPHVSSVPRAASQGVPTLQTSSPLSAPQEMIPTTETVPDLKPPVTGLSQPLRPLGPAAANVSILNNLSQARQVMNSAALAGGTSIGLQSMGGTHMAMHMSNMISSGMVSSVPPTQTVLSSGQSGMTSIAASGQPGMTSMAGTGALAGNAQVALNTNSGSFASASSNLSGNSNLGMSQPMGNTQGGVNMGQSVPVVSQANISSTQMAQNGIGMNTNMISGVSQSGISSANGTMMPTPGMGPQGQPGPQSLGMANNAAANMPIPSQASNAMQSAPSQSKYVRVWEGNLSGQRQGQPVFITRLEGYRSASASETLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPG 703
            MAEKQLIV VEGTAAMGP+W  ++++YLDK+I+ FC  + T QK S   VEFSLV FN  G+Y ACLVQRSGWT+DVD F QWLSAI FSGGGF+D AI+EGL+EALMM  P+  NG Q  QNVD QRHCILVAASNP+PLPTPV+RPQI  LEQ EN+E+Q ESR+SDAET+A+ F Q SVSLSVI PKQL KLKAIY+AGK+NPRAADPP+DNVK+   LVLISENF+E RAALSR+G+  L +N SP+K+D  SV  V T P       PP+SV   NGS++ R P+ VGNV    VK+EPTT+TSM +GPA+ HV SVPRA SQ +PTLQTSSPL+  QE + + + + +LKP V+G++QP RP+ PAAANV+ILNNLSQAR VM+SAAL GGTSIGLQSM  T +AMHMSNMISSGM SSVPP  TVLSSGQ           P MTS+ G+GAL G AQV  N+   SF+SA+SN++GNSN+G+SQPMGN QG VN+GQSVP +SQ N S TQM Q+G+GM+ NM S + QS +SS NGTM+PTPGM  Q Q G Q+LG++N++AA+MP+  Q S+A+QSA  QSKYV+VWEGNLSGQRQGQPVFITRLEGYRSASASETLAA+WP TMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLP QTLLLSVSDKACRLIGMLFPG
Sbjct:    1 MAEKQLIVAVEGTAAMGPYWHIVVSDYLDKIIRCFCSSELTGQKNSTSNVEFSLVTFNTHGSYCACLVQRSGWTKDVDIFLQWLSAIPFSGGGFNDAAIAEGLSEALMMF-PIASNGNQTPQNVDGQRHCILVAASNPYPLPTPVYRPQIQNLEQVENSEAQTESRLSDAETVARSFAQCSVSLSVICPKQLAKLKAIYSAGKQNPRAADPPVDNVKNSQFLVLISENFMEGRAALSRSGLPSLATNQSPVKMDMASVTSV-TGP-------PPSSV---NGSMVGRLPVSVGNVATATVKVEPTTITSMATGPAFSHVPSVPRAPSQAIPTLQTSSPLTNTQEGMTSGDNMQELKPSVSGMTQPSRPVPPAAANVNILNNLSQAR-VMSSAALTGGTSIGLQSMVQTPVAMHMSNMISSGMASSVPPAHTVLSSGQ-----------PTMTSLTGSGALTGTAQVLPNSGLSSFSSATSNVAGNSNIGISQPMGNVQGAVNIGQSVPGMSQGNHSGTQMMQSGVGMSQNM-SALGQSTVSSGNGTMIPTPGMSQQVQSGMQALGVSNSSAASMPLSQQTSSALQSA--QSKYVKVWEGNLSGQRQGQPVFITRLEGYRSASASETLAAHWPQTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPQQTLLLSVSDKACRLIGMLFPG 679    

HSP 2 Score: 38.891 bits (89), Expect = 4.315e+0
Identity = 17/24 (70.83%), Postives = 20/24 (83.33%), Query Frame = 0
 
Query:  702 PGGRQMASQGQVTSQGPPSMPGGG 725
            PG  Q+ SQGQV+SQ PP+MPGGG
Sbjct:  791 PGRSQLVSQGQVSSQAPPNMPGGG 814    
BLAST of DCAR_026720 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2C9UKC2 (Mediator of RNA polymerase II transcription subunit 25 {ECO:0000256|ARBA:ARBA00019694})

HSP 1 Score: 940.258 bits (2429), Expect = 0.000e+0
Identity = 496/706 (70.25%), Postives = 567/706 (80.31%), Query Frame = 0
 
Query:    1 MAEKQLIVVVEGTAAMGPHWQTILTEYLDKVIKSFCGYDSTTQKPS-VPVEFSLVVFNAPGNYSACLVQRSGWTRDVDNFFQWLSAISFSGGGFSDVAISEGLAEALMM--CTPLNGKQPHQNVDKQRHCILVAASNPFPLPTPVFRPQIPKLEQSENTESQKESRISDAETLAKFFPQLSVSLSVISPKQLPKLKAIYNAGKRNPRAADPPIDNVKSPYHLVLISENFIEARAALSRNGIAPLVSNHSPMKVDTTSVMKVDTSPVLPVSVSPPTSVPSANGSVMNRQPIIVGNVTPTIVKIEPTTVTSMVSGPAYPHVSSVPRAASQGVPTLQTSSPLSAPQEMIPTTETVPDLKPPVTGLSQPLRPLGPAAANVSILNNLSQARQVMNSAALAGGTSIGLQSMGGTHMAMHMSNMISSGMVSSVPPTQTVLSSGQSGMTSIAASGQPGMTSMAGTGALAGNAQVALNTNSGSFASASSNLSGNSNLGMSQPMGNTQGGVNMGQSVPVVSQANISSTQMAQNGIGMNTNMISGVSQSGISSANGTMMPTPGMGPQGQPGPQSLGMANNAAANMPIPSQASNAMQSAPSQSKYVRVWEGNLSGQRQGQPVFITRLEGYRSASASETLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPG 703
            MAEKQLIV VEG+AAMGP WQTIL+EYL+K+I+SFCG D T QKPS    E SLV F + G+Y ACLVQRSGWTRDVD F QWLSAI F+GGGF+D AI+EGL+EALMM  CT  NG Q  QN++ QRHC+L+AASNP PLPTPV+RPQI  LEQSEN + Q ESR+SDAET+AK FPQ SVSLSVI PKQLPK++AIY AGKRN RAADP +DNVK+P+ LVLISENF+EARA+LSR G+A L SN SP+K+D  SV     S   P     P+S+PS NGS+MNR P+ VG V    VK+EPTTVTSM  GPA+PH++SV R ASQ VP+LQTSSP +  QEMI + E VPDLKP V+G+ Q +RP+ P  ANVSILNNLSQARQVMNSAAL GGT+IGLQS+  T +AMHMSNMISSGM SSVP  Q V SSGQ G+TSIA SG                 QVA N+  GSF SA+SN+SGNSNLG+SQP+ N QGGV+MG SVP +SQ ++S  QM Q+GIGMN NM+SG+  SG+SS + TM+PTPG+  Q Q G QSLG+ NN+AANMP+  Q ++A+QSA  QSKYV+VWEGNLSGQRQGQPVFITRLEGYRSASASETLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPG
Sbjct:    1 MAEKQLIVAVEGSAAMGPFWQTILSEYLEKMIRSFCGSDLTGQKPSPSNAELSLVTFYSHGSYCACLVQRSGWTRDVDIFLQWLSAIPFAGGGFNDAAIAEGLSEALMMFPCT-QNGSQAQQNIEGQRHCVLIAASNPHPLPTPVYRPQIQNLEQSENIDPQTESRLSDAETVAKSFPQCSVSLSVICPKQLPKIRAIYTAGKRNIRAADPAVDNVKNPHFLVLISENFMEARASLSRPGVANLPSNQSPVKLDVASV----ASAAGPA----PSSIPSVNGSIMNRPPMSVGTVPTATVKVEPTTVTSMAPGPAFPHITSV-RPASQPVPSLQTSSPPTTTQEMIASGENVPDLKPSVSGMQQSVRPVPPGTANVSILNNLSQARQVMNSAALTGGTTIGLQSVTQTPVAMHMSNMISSGMASSVPAAQNVFSSGQPGITSIAGSG---------------TTQVAANSGLGSFTSATSNISGNSNLGISQPISNLQGGVSMGPSVPGMSQGSLSGAQMVQSGIGMNQNMMSGLGPSGVSSGSSTMIPTPGIPQQAQSGMQSLGVNNNSAANMPLSQQTTSALQSA--QSKYVKVWEGNLSGQRQGQPVFITRLEGYRSASASETLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPG 679    
BLAST of DCAR_026720 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6P4N5F1 (Mediator of RNA polymerase II transcription subunit 25 {ECO:0000256|ARBA:ARBA00019694})

HSP 1 Score: 940.258 bits (2429), Expect = 0.000e+0
Identity = 489/706 (69.26%), Postives = 570/706 (80.74%), Query Frame = 0
 
Query:    1 MAEKQLIVVVEGTAAMGPHWQTILTEYLDKVIKSFCGYDSTTQKPSVP-VEFSLVVFNAPGNYSACLVQRSGWTRDVDNFFQWLSAISFSGGGFSDVAISEGLAEALMMCTPL--NGKQPHQNVDKQRHCILVAASNPFPLPTPVFRPQIPKLEQSENTESQKESRISDAETLAKFFPQLSVSLSVISPKQLPKLKAIYNAGKRNPRAADPPIDNVKSPYHLVLISENFIEARAALSRNGIAPLVSNHSPMKVDTTSVMKVDTSPVLPVSVSPPTSVPSANGSVMNRQPIIVGNVTPTIVKIEPTTVTSMVSGPAYPHVSSVPRAASQGVPTLQTSSPLSAPQEMIPTTETVPDLKPPVTGLSQPLRPLGPAAANVSILNNLSQARQVMNSAALAGGTSIGLQSMGGTHMAMHMSNMISSGMVSSVPPTQTVLSSGQSGMTSIAASGQPGMTSMAGTGALAGNAQVALNTNSGSFASASSNLSGNSNLGMSQPMGNTQGGVNMGQSVPVVSQANISSTQMAQNGIGMNTNMISGVSQSGISSANGTMMPTPGMGPQGQPGPQSLGMANNAAANMPIPSQASNAMQSAPSQSKYVRVWEGNLSGQRQGQPVFITRLEGYRSASASETLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPG 703
            MAEKQLIV VEGTAAMGP+W  ++++YLDK+I+ FC  + T QK S   VEFSLV FN  G+Y ACLVQRSGWT+DVD F QWLSAI FSGGGF+D AI+EGL+EALMM  P+  NG Q  QNVD QRHCILVAASNP+PLPTPV+RPQI  LEQ EN+E+Q ESR+SDAET+A+ F Q SVSLSVI PKQL KLKAIY+AGK+NPRAADPP+DNVK+   LVLISENF+E RAALSR+G+  L +N SP+K+D  SV  V T P       PP+SV   NGS++ R P+ VGNV    VK+EPTT+TSM +GPA+ HV SVPRA SQ +PTLQTSSPL+  QE + + + + +LKP V+G++QP RP+ PAAANV+ILNNLSQAR VM+SAAL GGTSIGLQSM  T +AMHMSNMISSGM SSVPP  TVLSSGQ           P MTS+ G+GAL G AQV  N+   SF+SA+SN++GNSN+G+SQPMGN QG VN+GQSVP +SQ N S TQM Q+G+GM+ NM S + QS +SS NGTM+PTPGM  Q Q G Q+LG++N++AA+MP+  Q S+A+QSA  QSKYV+VWEGNLSGQRQGQPVFITRLEGYRSASASETLAA+WP TMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLP QTLLLSVSDKACRLIGMLFPG
Sbjct:    1 MAEKQLIVAVEGTAAMGPYWHIVVSDYLDKIIRCFCSSELTGQKNSTSNVEFSLVTFNTHGSYCACLVQRSGWTKDVDIFLQWLSAIPFSGGGFNDAAIAEGLSEALMMF-PIASNGNQTPQNVDGQRHCILVAASNPYPLPTPVYRPQIQNLEQVENSEAQTESRLSDAETVARSFAQCSVSLSVICPKQLAKLKAIYSAGKQNPRAADPPVDNVKNSQFLVLISENFMEGRAALSRSGLPSLATNQSPVKMDMASVTSV-TGP-------PPSSV---NGSMVGRLPVSVGNVATATVKVEPTTITSMATGPAFSHVPSVPRAPSQAIPTLQTSSPLTNTQEGMTSGDNMQELKPSVSGMTQPSRPVPPAAANVNILNNLSQAR-VMSSAALTGGTSIGLQSMVQTPVAMHMSNMISSGMASSVPPAHTVLSSGQ-----------PTMTSLTGSGALTGTAQVLPNSGLSSFSSATSNVAGNSNIGISQPMGNVQGAVNIGQSVPGMSQGNHSGTQMMQSGVGMSQNM-SALGQSTVSSGNGTMIPTPGMSQQVQSGMQALGVSNSSAASMPLSQQTSSALQSA--QSKYVKVWEGNLSGQRQGQPVFITRLEGYRSASASETLAAHWPQTMQIVRLISQDHMNNKQYVGKADFLVFRAMNQHGFLGQLQEKKLCAVIQLPQQTLLLSVSDKACRLIGMLFPG 679    

HSP 2 Score: 38.891 bits (89), Expect = 4.339e+0
Identity = 17/24 (70.83%), Postives = 20/24 (83.33%), Query Frame = 0
 
Query:  702 PGGRQMASQGQVTSQGPPSMPGGG 725
            PG  Q+ SQGQV+SQ PP+MPGGG
Sbjct:  814 PGRSQLVSQGQVSSQAPPNMPGGG 837    
Match NameStatsDescription
A0A5J5ANN6E-Value: 0.000e+0, PID: 72.14Mediator of RNA polymerase II transcription subuni... [more]
A0A5B7ATQ3E-Value: 3.039e+0, PID: 72.14Mediator of RNA polymerase II transcription subuni... [more]
A0A5B7AVZ0E-Value: 3.127e+0, PID: 72.14Mediator of RNA polymerase II transcription subuni... [more]
A0A2R6PH51E-Value: 1.204e+0, PID: 69.97Mediator of RNA polymerase II transcription subuni... [more]
A0A6P6BIP3E-Value: 1.953e+0, PID: 69.83Mediator of RNA polymerase II transcription subuni... [more]
A0A1R3J5Z7E-Value: 5.619e+0, PID: 68.66Mediator of RNA polymerase II transcription subuni... [more]
A0A5B6X4W5E-Value: 0.000e+0, PID: 66.31Mediator of RNA polymerase II transcription subuni... [more]
A0A6P4MG19E-Value: 4.315e+0, PID: 69.26Mediator of RNA polymerase II transcription subuni... [more]
A0A2C9UKC2E-Value: 0.000e+0, PID: 70.25Mediator of RNA polymerase II transcription subuni... [more]
A0A6P4N5F1E-Value: 4.339e+0, PID: 69.26Mediator of RNA polymerase II transcription subuni... [more]

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Analysis: 
NameDescription

An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4.

There are a few different naming schemes for this assembly. First there is the
Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below.
These sequences can be viewed in JBrowse here.

Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0

LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926.

Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence.

RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records.

The NCBI Sequence report lists the correspondences between the various naming methods

Link to the LNRQ01000000.1 master record at NCBI

Raw Reads: Link to SRA accessions used for the genome assembly

This genome is available in the CarrotOmics Blast Search

This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions.

For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins.

Data from this analysis can be viewed in JBrowse here.

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