MTVLEFPTKESRRTSGTTMIQDNVLAITDHVILPISVVLNAIKEIKTEGI
EQTDPLFSRVLRPGGQILFQTSQSTLDQVTTSSLQGKLLVAGYVDVQIGT
MTLTVPSEVSQPITVKAKRPSWKVGSSFSIKKAMKNLPKLQIDDDMDLID
EDSLLSEDDLKKPQIPPVGDCEVSNTRKACKNCSCGRAEAEQKVQKIGPT
MDQLENPQSACGSCGLGDAFRCGSCPYKGLPPFKLGEKVSLSGNFLAADI
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| Relationships |
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| The polypeptide, DCAR_030401, derives from mRNA, DCAR_030401. |
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
Match: A0A5B7A054 (Anamorsin homolog {ECO:0000256|HAMAP-Rule:MF_03115} (Fe-S cluster assembly protein DRE2 homolog {ECO:0000256|HAMAP-Rule:MF_03115}))
HSP 1 Score: 317.39 bits (812), Expect = 2.895e-107
Identity = 176/273 (64.47%), Postives = 202/273 (73.99%), Query Frame = 0
Query: 17 TTMIQDNVLAITDHVILPISVVLNAIKEIKTEGIEQTDPLF--------------------------------------SRVLRPGGQILF-QTSQSTLDQVTTSSLQGKLLVAGYVDVQIGTMTLTVPSEVSQPITVKAKRPSWKVGSSFSIKKAMKNLPKLQIDDDMDLIDEDSLLSEDDLKKPQIPPVGDCEVSNTRKACKNCSCGRAEAEQKVQKIGPTMDQLENPQSACGSCGLGDAFRCGSCPYKGLPPFKLGEKVSLSGNFLAADI 250
TT +Q NVLA+TDH I+PIS VLNAI+ IK EG+EQ+DPL SRVL+PGG IL QTSQST Q+TTSS + KLLVAG+VDVQ+ M VPSEV + +KAK+ SWK+GSSFSIKKA K+LPK+Q+DDDMDLIDEDSLL+E+DLKKPQ+P VGDCEV +TRKACKNC CGRAEAE+KVQK+G TM+QL PQSACGSCGLGDAFRC +CPYKGLPPFKLGEKVSL GNFLAADI
Sbjct: 3 TTTMQGNVLALTDHAIVPISAVLNAIRVIKNEGVEQSDPLIITQASSLSQLPLDSSSVDIVISICRSFDFPCDQLFGEISRVLKPGGTILLHQTSQSTTGQMTTSSFERKLLVAGFVDVQVIQMKPVVPSEVVKDFGIKAKKLSWKIGSSFSIKKATKHLPKVQVDDDMDLIDEDSLLTEEDLKKPQLPLVGDCEVGSTRKACKNCICGRAEAEEKVQKLGLTMEQLNTPQSACGSCGLGDAFRCSTCPYKGLPPFKLGEKVSLPGNFLAADI 275
Match: A5AI62 (Anamorsin homolog {ECO:0000256|HAMAP-Rule:MF_03115} (Fe-S cluster assembly protein DRE2 homolog {ECO:0000256|HAMAP-Rule:MF_03115}))
HSP 1 Score: 315.079 bits (806), Expect = 2.647e-106
Identity = 157/257 (61.09%), Postives = 195/257 (75.88%), Query Frame = 0
Query: 17 TTMIQDNVLAITDHVILPISVVLNAIKEIKTEGIEQTDPLF---------------------SRVLRPGGQILFQ-TSQSTLD-QVTTSSLQGKLLVAGYVDVQIGTMTLTVPSEVSQPITVKAKRPSWKVGSSFSIKKAMKNLPKLQIDDDMDLIDEDSLLSEDDLKKPQIPPVGDCEVSNTRKACKNCSCGRAEAEQKVQKIGPTMDQLENPQSACGSCGLGDAFRCGSCPYKGLPPFKLGEKVSLSGNFLAADI 250
T M+Q++ LA+TDH +L IS VL+A++++ G++Q DPL SRVL+PGG +L Q TSQS + +SL+ KLL+AG+++ Q + VPSE +Q +KAK+PSWK+GSSFSIKKA K+LPK QI+DDMDLIDEDSLL+E+DLKKPQ+P GDCEV +TRKACKNC+CGRAE E+KV+K+G TMDQL NPQSACGSCGLGDAFRCG+CPYKGLPPFKLG+KVSLSGNFL ADI
Sbjct: 23 TKMLQNSTLALTDHEVLXISTVLDAVRKLDNAGVDQCDPLIITQASSLNHLNFLVISCWQKSSRVLKPGGTVLIQKTSQSVAGIKDGETSLERKLLMAGFLEAQAXQLKPVVPSEGAQSFGIKAKKPSWKIGSSFSIKKAEKSLPKFQIEDDMDLIDEDSLLTEEDLKKPQLPVDGDCEVGSTRKACKNCTCGRAEEEEKVEKLGLTMDQLNNPQSACGSCGLGDAFRCGTCPYKGLPPFKLGDKVSLSGNFLVADI 279
Match: A0A2R6QX89 ((Anamorsin like {ECO:0000313|EMBL:PSS16361.1}) (Fragment))
HSP 1 Score: 306.605 bits (784), Expect = 4.862e-103
Identity = 158/274 (57.66%), Postives = 196/274 (71.53%), Query Frame = 0
Query: 17 TTMIQDNVLAITDHVILPISVVLNAIKEIKTEGIEQTDPL--------------------------------------FSRVLRPGGQILF-QTSQSTLDQVTTSSLQGKLLVAGYVDVQIGTMTLTVPSEVSQPITVKAKRPSWKVGSSFSIKKA-MKNLPKLQIDDDMDLIDEDSLLSEDDLKKPQIPPVGDCEVSNTRKACKNCSCGRAEAEQKVQKIGPTMDQLENPQSACGSCGLGDAFRCGSCPYKGLPPFKLGEKVSLSGNFLAADI 250
T +Q +VLA+TD +LPIS VLNAIK IK EG+E+ DPL SRVL+PGG +L Q+S+ + + TS L+ KLLVAG++DVQ + + S V Q + +KA++PSW++GSSF++KK K LPK+QID+DMDLIDEDSLL+E+DLKKPQ+PPVGDCEV +TRKACKNC+CGRAEAE+KVQK+ TMDQ+ NPQSACGSCGLGDAFRCG+CPY+GL PFKLGEKV+LSGNFLAADI
Sbjct: 2 TVKMQSSVLALTDLAVLPISAVLNAIKIIKNEGVEKIDPLVITQASLLGKLPVDLSSVDIIVFICRSSEFPGDQLFGEISRVLKPGGIVLLHQSSEFSAGEKITSPLERKLLVAGFLDVQAFPVKSVLQSNVVQSVGIKARKPSWQLGSSFALKKKPTKILPKVQIDEDMDLIDEDSLLTEEDLKKPQLPPVGDCEVGSTRKACKNCTCGRAEAEEKVQKLELTMDQMNNPQSACGSCGLGDAFRCGTCPYRGLAPFKLGEKVALSGNFLAADI 275
Match: D1I234 (Anamorsin homolog {ECO:0000255|HAMAP-Rule:MF_03115} (Fe-S cluster assembly protein DRE2 homolog {ECO:0000255|HAMAP-Rule:MF_03115}))
HSP 1 Score: 305.834 bits (782), Expect = 9.864e-103
Identity = 156/272 (57.35%), Postives = 194/272 (71.32%), Query Frame = 0
Query: 19 MIQDNVLAITDHVILPISVVLNAIKEIKTEGIEQTDPLF--------------------------------------SRVLRPGGQILFQ-TSQSTLD-QVTTSSLQGKLLVAGYVDVQIGTMTLTVPSEVSQPITVKAKRPSWKVGSSFSIKKAMKNLPKLQIDDDMDLIDEDSLLSEDDLKKPQIPPVGDCEVSNTRKACKNCSCGRAEAEQKVQKIGPTMDQLENPQSACGSCGLGDAFRCGSCPYKGLPPFKLGEKVSLSGNFLAADI 250
M+Q++ LA+TDH +L IS VL+A++++ G++Q DPL SRVL+PGG +L Q TSQS + +SL+ KLL+AG+++ Q + VPSE +Q +KAK+PSWK+GSSFSIKKA K+LPK QI+DDMDLIDEDSLL+E+DLKKPQ+P GDCEV +TRKACKNC+CGRAE E+KV+K+G TMDQL NPQSACGSCGLGDAFRCG+CPYKGLPPFKLG+KVSLSGNFL ADI
Sbjct: 1 MLQNSTLALTDHEVLSISTVLDAVRKLDNAGVDQCDPLIITQASSLSQLPVESSSLDIVISICRSLEFPCDKLLAEISRVLKPGGTVLIQKTSQSVAGIKDGETSLERKLLMAGFLEAQAIQLKPVVPSEGAQSFGIKAKKPSWKIGSSFSIKKAEKSLPKFQIEDDMDLIDEDSLLTEEDLKKPQLPVDGDCEVGSTRKACKNCTCGRAEEEEKVEKLGLTMDQLNNPQSACGSCGLGDAFRCGTCPYKGLPPFKLGDKVSLSGNFLVADI 272
Match: A0A7J7G4J9 (Anamorsin homolog {ECO:0000256|HAMAP-Rule:MF_03115} (Fe-S cluster assembly protein DRE2 homolog {ECO:0000256|HAMAP-Rule:MF_03115}))
HSP 1 Score: 305.449 bits (781), Expect = 2.471e-102
Identity = 167/274 (60.95%), Postives = 202/274 (73.72%), Query Frame = 0
Query: 17 TTMIQDNVLAITDHVILPISVVLNAIKEIKTEGIEQTDPLF--------------------------------------SRVLRPGGQILFQ-TSQSTLDQVTTSSLQGKLLVAGYVDVQIGTMTLTVPSEVSQPITVKAKRPSWKVGSSFSIK-KAMKNLPKLQIDDDMDLIDEDSLLSEDDLKKPQIPPVGDCEVSNTRKACKNCSCGRAEAEQKVQKIGPTMDQLENPQSACGSCGLGDAFRCGSCPYKGLPPFKLGEKVSLSGNFLAADI 250
TT +Q +VLA+TD +LPIS VLNAIK IK EG+EQ DPL SRVL+P G +L Q S+S Q+ TSSL+ KLLVAG++DVQ+ + +PSEV Q + +KA++PSWK+GSSFS+K K K+LPK+QIDDDMDLIDEDSLL+E+DLKKPQ+PP+GDCEV +TRKACKNCSCGRAE E+KVQK+G TMDQL+NPQSACGSCGLGDAFRCG+CPY+GLPPFKLG+KV+LSGNFLAAD
Sbjct: 12 TTAMQSSVLALTDLAVLPISAVLNAIKMIKNEGVEQIDPLIITQASLLSQLPVDLSSVDTIIFICRSFEFPGDQLFGEISRVLKPAGMVLLQQYSESAKGQMITSSLERKLLVAGFLDVQVFPVKSALPSEVVQSVGIKARKPSWKIGSSFSLKSKPTKSLPKVQIDDDMDLIDEDSLLTEEDLKKPQLPPIGDCEVGSTRKACKNCSCGRAEEEEKVQKLGLTMDQLDNPQSACGSCGLGDAFRCGTCPYRGLPPFKLGDKVTLSGNFLAADF 285
Match: A0A5J5BMX8 (Anamorsin homolog {ECO:0000256|HAMAP-Rule:MF_03115} (Fe-S cluster assembly protein DRE2 homolog {ECO:0000256|HAMAP-Rule:MF_03115}))
HSP 1 Score: 303.523 bits (776), Expect = 9.397e-102
Identity = 166/273 (60.81%), Postives = 198/273 (72.53%), Query Frame = 0
Query: 17 TTMIQDNVLAITDHVILPISVVLNAIKEIKTEGIEQTDPLF--------------------------------------SRVLRPGGQILF-QTSQSTLDQVTTSSLQGKLLVAGYVDVQIGTMTLTVPSEVSQPITVKAKRPSWKVGSSFSIKKAMKNLPKLQIDDDMDLIDEDSLLSEDDLKKPQIPPVGDCEVSNTRKACKNCSCGRAEAEQKVQKIGPTMDQLENPQSACGSCGLGDAFRCGSCPYKGLPPFKLGEKVSLSGNFLAADI 250
TTM Q +VLA+TDH ++PIS VLNAI+ IK E ++ +DPL SRVL+PGG ILF QTSQS ++TTSS + KLLVAG++D+Q+ M VPSEV Q +KAK+PSWK+GSSFSI+K K+LPK+ IDDDMDLIDEDSLL+E+DLKKPQ+P VGDCEV +TRKACKNC CGRAE E+KVQK+G +MDQL NPQSACG+CGLGDAFRC +CPYKGLP FKLGEKV+LSGNFL ADI
Sbjct: 3 TTMTQGSVLALTDHAMVPISAVLNAIRVIKNEAVDNSDPLIITQASSLSQLPLDSSSVDSVISICRSFEFPGDKLFGEISRVLKPGGIILFHQTSQSASGKMTTSSFERKLLVAGFLDIQVIQMKPVVPSEVVQDFGIKAKKPSWKIGSSFSIRKTTKSLPKVHIDDDMDLIDEDSLLTEEDLKKPQLPLVGDCEVGSTRKACKNCICGRAEEEEKVQKLGLSMDQLNNPQSACGNCGLGDAFRCSTCPYKGLPTFKLGEKVALSGNFLVADI 275
Match: A0A7J0DYY6 (Anamorsin homolog {ECO:0000256|HAMAP-Rule:MF_03115} (Fe-S cluster assembly protein DRE2 homolog {ECO:0000256|HAMAP-Rule:MF_03115}))
HSP 1 Score: 302.368 bits (773), Expect = 2.449e-101
Identity = 156/271 (57.56%), Postives = 194/271 (71.59%), Query Frame = 0
Query: 20 IQDNVLAITDHVILPISVVLNAIKEIKTEGIEQTDPL--------------------------------------FSRVLRPGGQILF-QTSQSTLDQVTTSSLQGKLLVAGYVDVQIGTMTLTVPSEVSQPITVKAKRPSWKVGSSFSIKKA-MKNLPKLQIDDDMDLIDEDSLLSEDDLKKPQIPPVGDCEVSNTRKACKNCSCGRAEAEQKVQKIGPTMDQLENPQSACGSCGLGDAFRCGSCPYKGLPPFKLGEKVSLSGNFLAADI 250
+Q +VLA+TD +LPIS VLNAIK IK G+E+ DPL SRVL+PGG +L Q+S+ + + TS L+ KLLVAG++DVQ + + S V Q + +KA++PSW++GSSF++KK K LPK+QID+DMDLIDEDSLL+E+DLKKPQ+PPVGDCEV +TRKACKNC+CGRAEAE+KVQK+ TMDQ+ NPQSACGSCGLGDAFRCG+CPY+GL PFKLGEKV+LSGNFLAADI
Sbjct: 1 MQSSVLALTDLAVLPISAVLNAIKIIKNGGVEKIDPLVITQASLLGKLPVDLSSVDIIVFICRSSEFPGDQLFGEISRVLKPGGIVLLHQSSEFSAGEKITSPLERKLLVAGFLDVQAFPVKSVLQSNVVQSVGIKARKPSWQLGSSFALKKKPTKILPKVQIDEDMDLIDEDSLLTEEDLKKPQLPPVGDCEVGSTRKACKNCTCGRAEAEEKVQKLELTMDQMNNPQSACGSCGLGDAFRCGTCPYRGLAPFKLGEKVALSGNFLAADI 271
Match: A0A5B6ZXH5 (Methyltransf_11 domain-containing protein {ECO:0000259|Pfam:PF08241} (Fragment))
HSP 1 Score: 305.064 bits (780), Expect = 4.827e-101
Identity = 169/273 (61.90%), Postives = 199/273 (72.89%), Query Frame = 0
Query: 17 TTMIQDNVLAITDHVILPISVVLNAIKEIKTEGIEQTDPLF--------------------------------------SRVLRPGGQILF-QTSQSTLDQVTTSSLQGKLLVAGYVDVQIGTMTLTVPSEVSQPITVKAKRPSWKVGSSFSIKKAMKNLPKLQIDDDMDLIDEDSLLSEDDLKKPQIPPVGDCEVSNTRKACKNCSCGRAEAEQKVQKIGPTMDQLENPQSACGSCGLGDAFRCGSCPYKGLPPFKLGEKVSLSGNFLAADI 250
T +Q +VLA+TDH ++PIS VLNAI+ IKTE +EQ+DPL SRVL+PGG ILF QTSQS ++TTSS + KLLVAG++D+Q+ M VPSEV Q VKAK+PSWK+GSSFSIKKA K+LPK+QIDDDMDLIDEDSLL+E+DLKKPQ+P V DCEV +TRKACKNC+CGRAE E+KV K+G TMDQL NPQSACG+CGLGDAFRC +CPYKGLP F LGEKV+LSGNFL ADI
Sbjct: 101 TAKMQGSVLALTDHAMVPISAVLNAIRVIKTEAVEQSDPLIITQASSLSQLPLDSSSLDIVISICRSFEFPGDQLFGEISRVLKPGGIILFHQTSQSATGKMTTSSFERKLLVAGFLDIQVIQMKPVVPSEVGQDFGVKAKKPSWKIGSSFSIKKASKSLPKVQIDDDMDLIDEDSLLTEEDLKKPQLPLVDDCEVGSTRKACKNCTCGRAEEEEKVLKLGLTMDQLSNPQSACGNCGLGDAFRCSTCPYKGLPRFNLGEKVTLSGNFLDADI 373
Match: A0A103XR95 ((Cytokine-induced anti-apoptosis inhibitor 1 {ECO:0000313|EMBL:KVH95428.1}) (Fragment))
HSP 1 Score: 302.753 bits (774), Expect = 1.405e-100
Identity = 150/272 (55.15%), Postives = 192/272 (70.59%), Query Frame = 0
Query: 17 TTMIQDNVLAITDHVILPISVVLNAIKEIKTEGIEQTDPL--------------------------------------FSRVLRPGGQILFQTSQSTLDQVTTSSLQGKLLVAGYVDVQIGTMTLTVPSEVSQPITVKAKRPSWKVGSSFSIKKAMKNLPKLQIDDDMDLIDEDSLLSEDDLKKPQIPPVGDCEVSNTRKACKNCSCGRAEAEQKVQKIGPTMDQLENPQSACGSCGLGDAFRCGSCPYKGLPPFKLGEKVSLSGNFLAADI 250
T ++Q +VLAITD V +P++ VLNA++ +KTE IE+ +PL FSRVL+PGG+I + + +++ SSL+ KLLV G+ D+Q+ M + +K K+P+WK+GSSFS+KK +K+LPK+QID+DMDLIDEDSLLSE+DLKKP++PPVGDCEV +TRKACKNCSCGRAE E+KVQK+G TMDQLENPQSACGSCGLGDAFRC +CPYKGLPPF+LG+KV+LS NFL ADI
Sbjct: 75 TKVLQGSVLAITDSVAVPLNDVLNAVRVVKTEAIEEINPLVITQASSLSSLPVDTSSTDLIISISKSSVFSSDQLFVEFSRVLKPGGEIFIHQNSAVDKEMSKSSLERKLLVLGFSDIQVVQMA------EPESFGIKGKKPNWKIGSSFSLKKPVKSLPKVQIDNDMDLIDEDSLLSEEDLKKPELPPVGDCEVGSTRKACKNCSCGRAEEEEKVQKLGVTMDQLENPQSACGSCGLGDAFRCSTCPYKGLPPFQLGQKVTLSENFLVADI 340
Match: A0A5N6MML9 (Anamorsin homolog {ECO:0000256|HAMAP-Rule:MF_03115} (Fe-S cluster assembly protein DRE2 homolog {ECO:0000256|HAMAP-Rule:MF_03115}))
HSP 1 Score: 295.049 bits (754), Expect = 1.317e-98
Identity = 147/270 (54.44%), Postives = 186/270 (68.89%), Query Frame = 0
Query: 19 MIQDNVLAITDHVILPISVVLNAIKEIKTEGIEQTDPL--------------------------------------FSRVLRPGGQILFQTSQSTLDQVTTSSLQGKLLVAGYVDVQIGTMTLTVPSEVSQPITVKAKRPSWKVGSSFSIKKAMKNLPKLQIDDDMDLIDEDSLLSEDDLKKPQIPPVGDCEVSNTRKACKNCSCGRAEAEQKVQKIGPTMDQLENPQSACGSCGLGDAFRCGSCPYKGLPPFKLGEKVSLSGNFLAADI 250
M+Q VLAITD+V+L ++ VLN ++ +K E +E+ +P+ FSRVL+PGG+I + + SSL+ KLLV G+ D+Q+ V Q +K K+P+WK+GSSFS+KK +K+LPK+QI DDMDLIDEDSLLSE+DLKKPQ+PPVGDCEV +TRKACKNC+CGRAE E+KVQK+G TMD LENP+SACG+CGLGDAFRCG+CPYKGLPPFKLG+KV+LS NFLAADI
Sbjct: 1 MLQGGVLAITDNVVLSLNEVLNVVRVVKNEAVEEINPMIVTQASSLSSLPVDSLSIDLVTLISKSSDFSGDKLFEEFSRVLKPGGEIFIYQTSDVAKETVKSSLERKLLVTGFSDIQV------VQKAEIQSFGIKGKKPAWKIGSSFSLKKPVKSLPKVQIADDMDLIDEDSLLSEEDLKKPQLPPVGDCEVGSTRKACKNCTCGRAEEEEKVQKLGVTMD-LENPKSACGNCGLGDAFRCGTCPYKGLPPFKLGQKVTLSNNFLAADI 263
| Match Name | Stats | Description |
|---|---|---|
| A0A5B7A054 | E-Value: 2.895e-107, PID: 64.47 | Anamorsin homolog {ECO:0000256|HAMAP-Rule:MF_03115... [more] |
| A5AI62 | E-Value: 2.647e-106, PID: 61.09 | Anamorsin homolog {ECO:0000256|HAMAP-Rule:MF_03115... [more] |
| A0A2R6QX89 | E-Value: 4.862e-103, PID: 57.66 | (Anamorsin like {ECO:0000313|EMBL:PSS16361.1}) (Fr... [more] |
| D1I234 | E-Value: 9.864e-103, PID: 57.35 | Anamorsin homolog {ECO:0000255|HAMAP-Rule:MF_03115... [more] |
| A0A7J7G4J9 | E-Value: 2.471e-102, PID: 60.95 | Anamorsin homolog {ECO:0000256|HAMAP-Rule:MF_03115... [more] |
| A0A5J5BMX8 | E-Value: 9.397e-102, PID: 60.81 | Anamorsin homolog {ECO:0000256|HAMAP-Rule:MF_03115... [more] |
| A0A7J0DYY6 | E-Value: 2.449e-101, PID: 57.56 | Anamorsin homolog {ECO:0000256|HAMAP-Rule:MF_03115... [more] |
| A0A5B6ZXH5 | E-Value: 4.827e-101, PID: 61.90 | Methyltransf_11 domain-containing protein {ECO:000... [more] |
| A0A103XR95 | E-Value: 1.405e-100, PID: 55.15 | (Cytokine-induced anti-apoptosis inhibitor 1 {ECO:... [more] |
| A0A5N6MML9 | E-Value: 1.317e-98, PID: 54.44 | Anamorsin homolog {ECO:0000256|HAMAP-Rule:MF_03115... [more] |
Pages
back to top| Name | Description |
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An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions. | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |