MVKETEYYDVLGVIPAASEEEIRKAYYLKARQVHPDKNPNDPRAAERFQA
SEAYQVLSDPVQRDAYDRNGKYCISKDTMLDPTAVFALLFGSELFEDYIG
HLAVASMASSELASESDNPERLNEKLKASKSIKLKDTQVYRRKAVQKDRE
DKLAGKLKNILQQYVRGDREGFQHFAETEAERLSHTAFGVNILHTIGYIY
ERQAAQELGKKAIYLGVPFMAEWVRNKGHFWKSQITAARGAFQLLQLQED
IRRQFKMDGSGPENDLESHLRLNKDTLMNSLWKLNVVDIEVTLLHVILET
GVKKEELKLRASALKLLGKIFQRDKYAQTSGTSKKKVAETSSDSSDDEDT
PRALNYRAPLVTQKIDKGSEKPDNGGRLVFEEKKLEGSGEDEFAALMQSC
MASHCLHFASSAHNYNSTFKSCCSSPPSAVTFSDRGSSYNPLVFQAVRLL
GPPARFEASKLKVEFKGEEPHRYTRIVPRSYTLSHCDFTANLTLTISRLV
HLDQLKGWYNKDDVVAQWTEVNGNVCLDVHCYVSGPNQLLDLAAEFRYHI
FSKELPLVLMAVLHGDSAFFSEHQDLMNASVRVYFHSSSKKYNRVESWGA
LKDAAQERRGDHLKHSLLAAKEVPHHQKNCGTPKSLFQALVAFLL*
| Relationships |
|---|
| The polypeptide, DCAR_014396, derives from mRNA, DCAR_014396. |
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
Match: A0A1U8B2Y4 ((chaperone protein dnaJ 10 {ECO:0000313|RefSeq:XP_010270365.1}))
HSP 1 Score: 564.303 bits (1453), Expect = 0.000e+0
Identity = 280/378 (74.07%), Postives = 319/378 (84.39%), Query Frame = 0
Query: 1 MVKETEYYDVLGVIPAASEEEIRKAYYLKARQVHPDKNPNDPRAAERFQA-SEAYQVLSDPVQRDAYDRNGKYCISKDTMLDPTAVFALLFGSELFEDYIGHLAVASMASSELASESDNPERLNEKLKASKSIKLKDTQVYRRKAVQKDREDKLAGKLKNILQQYVRGDREGFQHFAETEAERLSHTAFGVNILHTIGYIYERQAAQELGKKAIYLGVPFMAEWVRNKGHFWKSQITAARGAFQLLQLQEDIRRQFKMDGSGPENDLESHLRLNKDTLMNSLWKLNVVDIEVTLLH----VILETGVKKEELKLRASALKLLGKIFQRDKYAQTSGTSKKKVAE------TSSDSSDDEDTPRALNYRAPLVTQKIDK 367
MVK+TEYYDVLGV P+ASEEEIRKAYYLKARQVHPDKNPNDP+AAERFQ EAYQVLSDPVQR+AYD+NGKYCIS++TMLDPTAVFALLFGSELFEDY+GHLAVASMASSELA+E DNPE+L+++LKA VQK+RE+KLA LK+ L +YV GD+EGF H A +EA+RLS AFG +ILHTIGYIYERQAAQ+LGKKAIYLGVPF+AEWVRNKGHFWKSQITAA+GAFQLLQLQED+RRQFKMDGSGPEN+LESH++LNKDTLMNSLWKLNVVDIEVTLLH V+ ET +KKEELK RA ALK+LGKIFQR+K +Q G SKKK +SSDS+ ED+PR L+YR PL+TQ I +
Sbjct: 1 MVKDTEYYDVLGVSPSASEEEIRKAYYLKARQVHPDKNPNDPQAAERFQVLGEAYQVLSDPVQREAYDQNGKYCISRETMLDPTAVFALLFGSELFEDYVGHLAVASMASSELATECDNPEKLHDRLKA----------------VQKEREEKLARILKDFLNRYVHGDKEGFIHHAASEAKRLSDAAFGADILHTIGYIYERQAAQQLGKKAIYLGVPFLAEWVRNKGHFWKSQITAAKGAFQLLQLQEDVRRQFKMDGSGPENNLESHMQLNKDTLMNSLWKLNVVDIEVTLLHVCQMVLQETNIKKEELKARALALKILGKIFQREKNSQNGGVSKKKNVSDAYEDGSSSDSTSVEDSPRTLSYRTPLITQGIGR 362
Match: A0A5J5B998 (J domain-containing protein {ECO:0000259|PROSITE:PS50076})
HSP 1 Score: 553.132 bits (1424), Expect = 0.000e+0
Identity = 282/381 (74.02%), Postives = 317/381 (83.20%), Query Frame = 0
Query: 1 MVKETEYYDVLGVIPAASEEEIRKAYYLKARQVHPDKNPNDPRAAERFQA-SEAYQVLSDPVQRDAYDRNGKYCISKDTMLDPTAVFALLFGSELFEDYIGHLAVASMASSELASESDNPERLNEKLKASKSIKLKDTQVYRRKAVQKDREDKLAGKLKNILQQYVRGDREGFQHFAETEAERLSHTAFGVNILHTIGYIYERQAAQELGKKAIYLGVPFMAEWVRNKGHFWKSQITAARGAFQLLQLQEDIRRQFKMDGSGPENDLESHLRLNKDTLMNSLWKLNVVDIEVTLLH----VILETGVKKEELKLRASALKLLGKIFQRDKYAQTSGTSKKKVAETSSDSSDD---------EDTPRALNYRAPLVTQKIDK 367
MVKET+YYD+LGV P+AS+E+IRKAYYLKARQVHPDKNP DP+AAERFQ EAYQVLSDPVQRD+YDRNGKYCIS++TMLDPTAVF LLFGSELFEDYIGHLAVASMASSELA ESDNPE+L+++LK VQK+RE+KLA LK+ L QYVRGDRE + AE+EAERLSH AFGV+ILHTIGYIY RQAAQELGKKAIYLGVPF+AEWVRNKGHFWKSQITAA+GAFQLLQLQE+ RRQFKMDGSGPEND+ESHLRLNKDTLM+SLWKLNVVDIEVTLLH V+ E+ VKKEELK RA ALK+LGKIFQR+K ++ GTSKKK+A SD + D ED+PR LNYR PL+TQ I +
Sbjct: 1 MVKETDYYDILGVSPSASDEDIRKAYYLKARQVHPDKNPYDPQAAERFQVLGEAYQVLSDPVQRDSYDRNGKYCISRETMLDPTAVFTLLFGSELFEDYIGHLAVASMASSELAGESDNPEKLHDRLKD----------------VQKEREEKLARTLKDFLHQYVRGDREEYLFRAESEAERLSHAAFGVDILHTIGYIYARQAAQELGKKAIYLGVPFLAEWVRNKGHFWKSQITAAKGAFQLLQLQEETRRQFKMDGSGPENDVESHLRLNKDTLMDSLWKLNVVDIEVTLLHVCQMVLKESNVKKEELKARAMALKILGKIFQREKQTRSGGTSKKKIA---SDINADGSSSDSSDEEDSPRTLNYRTPLITQGIGR 362
Match: E0CQK2 (J domain-containing protein {ECO:0000259|PROSITE:PS50076})
HSP 1 Score: 553.132 bits (1424), Expect = 0.000e+0
Identity = 287/386 (74.35%), Postives = 324/386 (83.94%), Query Frame = 0
Query: 1 MVKETEYYDVLGVIPAASEEEIRKAYYLKARQVHPDKNPNDPRAAERFQA-SEAYQVLSDPVQRDAYDRNGKYCISKDTMLDPTAVFALLFGSELFEDYIGHLAVASMASSELASESDNPERLNEKLKA--------SKSIKLKDTQVYRRKAVQKDREDKLAGKLKNILQQYVRGDREGFQHFAETEAERLSHTAFGVNILHTIGYIYERQAAQELGKKAIYLGVPFMAEWVRNKGHFWKSQITAARGAFQLLQLQEDIRRQFKMDGSGPENDLESHLRLNKDTLMNSLWKLNVVDIEVTLLH----VILETGVKKEELKLRASALKLLGKIFQRDKYAQTSGTSKKK------VAETSSDSSDDEDTPRALNYRAPLVTQKIDK 367
MVKETEYYDVLGV P ASEEEIRKAYYLKA+QVHPDKNP+DP AAERFQ EAYQVLSDP+QRDAYDRNGKYCISK+TMLDPTAVFALLFGSELFE+YIGHLAVASMASSELA ESDNPE+L++KLKA + I L + R+AVQK+RE++LA LK+ L QYV+GD+ GF H AE+EA+RLS FG +ILHTIGY+Y RQAAQELGKK IYLGVPF+AEWVRNKGHFWKSQITAA+GAFQLLQLQEDIRRQFKMDGSGPEND+ESH+R+NKDTLMNSLWKLNVVDIEVTL+H V+ E VKKEELK RA ALKLLGKIFQR+K A++ GTSK + +SSDSS +EDTPRAL+YR PL+TQ I +
Sbjct: 1 MVKETEYYDVLGVSPTASEEEIRKAYYLKAKQVHPDKNPSDPLAAERFQVLGEAYQVLSDPMQRDAYDRNGKYCISKETMLDPTAVFALLFGSELFENYIGHLAVASMASSELAEESDNPEKLHDKLKACLLGFCALTVCINL---LLCIREAVQKEREERLARLLKDFLNQYVQGDKRGFLHCAESEAKRLSDAVFGADILHTIGYVYARQAAQELGKKVIYLGVPFLAEWVRNKGHFWKSQITAAKGAFQLLQLQEDIRRQFKMDGSGPENDVESHIRMNKDTLMNSLWKLNVVDIEVTLIHVCQLVLQENNVKKEELKARALALKLLGKIFQREKLARSGGTSKSRNVSDINEEGSSSDSSGEEDTPRALSYRTPLITQGIGR 383
Match: A0A2G9H9D4 ((Molecular chaperone (DnaJ superfamily) {ECO:0000313|EMBL:PIN14125.1}))
HSP 1 Score: 550.051 bits (1416), Expect = 0.000e+0
Identity = 284/377 (75.33%), Postives = 321/377 (85.15%), Query Frame = 0
Query: 1 MVKETEYYDVLGVIPAASEEEIRKAYYLKARQVHPDKNPNDPRAAERFQA-SEAYQVLSDPVQRDAYDRNGKYCISKDTMLDPTAVFALLFGSELFEDYIGHLAVASMASSELASESDNPERLNEKLKASKSIKLKDTQVYRRKAVQKDREDKLAGKLKNILQQYVRGDREGFQHFAETEAERLSHTAFGVNILHTIGYIYERQAAQELGKKAIYLGVPFMAEWVRNKGHFWKSQITAARGAFQLLQLQEDIRRQFKMDGSGPENDLESHLRLNKDTLMNSLWKLNVVDIEVTLLH----VILETGVKKEELKLRASALKLLGKIFQRDKYAQTSGTSKKKVA-----ETSSDSSDDEDTPRALNYRAPLVTQKIDK 367
MVKETEYYD+LGV P+ASEEEIRKAYYLKARQVHPDKNPNDP+AAERFQ EAYQVLSDP+QRD YDRNGK+CISK+TMLDPTAVFALLFGSELFEDYIGHLAVASMAS+ELA ESDNPE+++++LK + L KAVQ++RE+KLA KL+N L QYV GDREGF AE+EA+RLSH AFG +LHTIGYIY RQAA +LGKKAIYLGVPF+AEWVRNKGHFWKSQ TAA+GAFQLLQLQED+RR FK+DGSGPEND ESHLRLNKDTLMNSLWKLNVVDIEVTLLH V+ E +KKEELKLRA ALK+LGK+FQ K Q +GTSKKK A ++SSDSSD+ED+PR+LNYR PL+TQ I +
Sbjct: 1 MVKETEYYDILGVSPSASEEEIRKAYYLKARQVHPDKNPNDPQAAERFQVLGEAYQVLSDPLQRDTYDRNGKHCISKETMLDPTAVFALLFGSELFEDYIGHLAVASMASTELAGESDNPEKVHDRLKFTHHSML-------LKAVQREREEKLARKLRNFLHQYVHGDREGFLKQAESEAQRLSHAAFGAEMLHTIGYIYTRQAALQLGKKAIYLGVPFVAEWVRNKGHFWKSQFTAAKGAFQLLQLQEDVRRHFKLDGSGPENDAESHLRLNKDTLMNSLWKLNVVDIEVTLLHVCQMVLQENCIKKEELKLRAVALKILGKVFQSQKDLQNAGTSKKKTALADADDSSSDSSDEEDSPRSLNYRTPLLTQGIGR 370
Match: A0A6I9T3D1 ((chaperone protein dnaJ 10 {ECO:0000313|RefSeq:XP_011074793.1}))
HSP 1 Score: 546.584 bits (1407), Expect = 0.000e+0
Identity = 278/377 (73.74%), Postives = 317/377 (84.08%), Query Frame = 0
Query: 1 MVKETEYYDVLGVIPAASEEEIRKAYYLKARQVHPDKNPNDPRAAERFQA-SEAYQVLSDPVQRDAYDRNGKYCISKDTMLDPTAVFALLFGSELFEDYIGHLAVASMASSELASESDNPERLNEKLKASKSIKLKDTQVYRRKAVQKDREDKLAGKLKNILQQYVRGDREGFQHFAETEAERLSHTAFGVNILHTIGYIYERQAAQELGKKAIYLGVPFMAEWVRNKGHFWKSQITAARGAFQLLQLQEDIRRQFKMDGSGPENDLESHLRLNKDTLMNSLWKLNVVDIEVTLLH----VILETGVKKEELKLRASALKLLGKIFQRDKYAQTSGTSKKKV-----AETSSDSSDDEDTPRALNYRAPLVTQKIDK 367
MVKETEYYD+LGV P+ASEEEIRKAYYLKARQVHPDKNPNDP+AAERFQ EAYQVLSDPVQRD YDRNGKYCISK+TMLDPTAVFALLFGSELFEDYIGHLAVASMAS+E A ESDNP++++++LK VQ++RE+KLA KL++ L YVRGD+EGF AE+EAERLSH AFG ILHTIGYIY RQAA +LGKKAIYLGVPF+AEWVRNKGHFWKSQ TAA+GAFQLLQLQEDIRRQFKMDGS PEND+ESHLRLNKDTLMNSLWKLNVVDIE+TLLH V+ + V+KEEL+LRA ALK+LGK+FQ K Q +GTSKKK+ ++SSDSSD++D+PR+LNYR PL+TQ I +
Sbjct: 1 MVKETEYYDILGVSPSASEEEIRKAYYLKARQVHPDKNPNDPQAAERFQVLGEAYQVLSDPVQRDTYDRNGKYCISKETMLDPTAVFALLFGSELFEDYIGHLAVASMASTEFAGESDNPDKVHDRLKV----------------VQREREEKLARKLRDFLHHYVRGDKEGFLKQAESEAERLSHAAFGAEILHTIGYIYTRQAALQLGKKAIYLGVPFVAEWVRNKGHFWKSQFTAAKGAFQLLQLQEDIRRQFKMDGSSPENDMESHLRLNKDTLMNSLWKLNVVDIEITLLHVCQMVLQDNSVRKEELRLRAVALKILGKVFQSQKNMQNAGTSKKKIASADADDSSSDSSDEDDSPRSLNYRTPLLTQGIGR 361
Match: A0A5N6RQ76 (J domain-containing protein {ECO:0000259|PROSITE:PS50076})
HSP 1 Score: 544.658 bits (1402), Expect = 0.000e+0
Identity = 283/375 (75.47%), Postives = 319/375 (85.07%), Query Frame = 0
Query: 1 MVKETEYYDVLGVIPAASEEEIRKAYYLKARQVHPDKNPNDPRAAERFQASEAYQVLSDPVQRDAYDRNGKYCISKDTMLDPTAVFALLFGSELFEDYIGHLAVASMASSELASESDNPERLNEKLKASKSIKLKDTQVYRRKAVQKDREDKLAGKLKNILQQYVRGDREGFQHFAETEAERLSHTAFGVNILHTIGYIYERQAAQELGKKAIYLGVPFMAEWVRNKGHFWKSQITAARGAFQLLQLQEDIRRQFKMDGSGPENDLESHLRLNKDTLMNSLWKLNVVDIEVTLLH----VILETGVKKEELKLRASALKLLGKIFQRDKYAQT--SGTSKKK------VAETSSDSSDDEDTPRALNYRAPLVTQ 363
MVKETEYYDVLGV P+ASEEEIRKAYYLKA+QVHPDKNPNDP+AAERFQASEAYQVLSDPVQ+DAYDRNGK CIS++TMLDP AVFALLFGSELFEDYIGHLAVASMASSELA ESDNPE+L+E+LKAS K+RE+KLA LK+ L QYV+GD+EGF AE+EA RLS TAFGV+ILHTIGYIY RQAAQELGKKAIYLGVPF+AEWVRNKGHFWKSQ+TAA+GAFQLLQLQEDI RQFK+DGSGPEND+ESH+RLNKDTLMNSLWKLNVVDIEVTL+H V+ E+ V+KEELK RA ALK+LGKIFQ +K+ Q+ GTSKKK +SSD+S D+D+P AL+YRAPL+TQ
Sbjct: 18 MVKETEYYDVLGVSPSASEEEIRKAYYLKAKQVHPDKNPNDPQAAERFQASEAYQVLSDPVQKDAYDRNGKNCISRETMLDPMAVFALLFGSELFEDYIGHLAVASMASSELAVESDNPEKLHERLKAS----------------SKEREEKLARLLKSFLNQYVQGDKEGFLQHAESEARRLSDTAFGVDILHTIGYIYSRQAAQELGKKAIYLGVPFLAEWVRNKGHFWKSQMTAAKGAFQLLQLQEDIHRQFKLDGSGPENDVESHIRLNKDTLMNSLWKLNVVDIEVTLMHVCQMVLRESHVRKEELKARAMALKILGKIFQ-EKHVQSGGGGTSKKKDGADTDDDGSSSDTSSDDDSPGALSYRAPLLTQ 375
Match: A0A6A4KUP0 (J domain-containing protein {ECO:0000259|PROSITE:PS50076} (Fragment))
HSP 1 Score: 544.273 bits (1401), Expect = 0.000e+0
Identity = 284/399 (71.18%), Postives = 318/399 (79.70%), Query Frame = 0
Query: 1 MVKETEYYDVLGVIPAASEEEIRKAYYLKARQVHPDKNPNDPRAAERFQAS---------------------EAYQVLSDPVQRDAYDRNGKYCISKDTMLDPTAVFALLFGSELFEDYIGHLAVASMASSELASESDNPERLNEKLKASKSIKLKDTQVYRRKAVQKDREDKLAGKLKNILQQYVRGDREGFQHFAETEAERLSHTAFGVNILHTIGYIYERQAAQELGKKAIYLGVPFMAEWVRNKGHFWKSQITAARGAFQLLQLQEDIRRQFKMDGSGPENDLESHLRLNKDTLMNSLWKLNVVDIEVTLLH----VILETGVKKEELKLRASALKLLGKIFQRDKYAQTSGTSKKKVAE-------TSSDSSDDEDTPRALNYRAPLVTQKIDK 367
MVKETEYYD LGV P+ASEEEIRKAYYLKARQVHPDKNPNDP+AAERFQAS EAYQVLSDP QRDAYDRNGKYCIS++TM+DPTAVFALLFGSELFEDY+GHLAVA+MASSEL+ E DNPE++++KLKAS Q+Y A QK+RE+KLA LK +LQQYVRGD+ GF AE+EAERLSH AFG +ILHTIGYIY RQAAQELGKKA YLGVPF+AEWVRNKGHFWKSQITAA+GAFQLLQLQED RRQFKMDGSGPENDL+SHLRLNKDTLMNSLWKLNVVDIEVTLLH V+ E+ V++E LK RA ALK LGKIFQR+K Q GTSKK + +S S D+ED+PR LNYR P++TQ I +
Sbjct: 1 MVKETEYYDALGVTPSASEEEIRKAYYLKARQVHPDKNPNDPQAAERFQASPLFPIHLCFIFLLVQISRVLGEAYQVLSDPGQRDAYDRNGKYCISRETMVDPTAVFALLFGSELFEDYVGHLAVATMASSELSGEIDNPEKVHDKLKASM------FQIYH--AFQKEREEKLARNLKGLLQQYVRGDKVGFVRCAESEAERLSHAAFGADILHTIGYIYARQAAQELGKKAKYLGVPFVAEWVRNKGHFWKSQITAAKGAFQLLQLQEDTRRQFKMDGSGPENDLQSHLRLNKDTLMNSLWKLNVVDIEVTLLHVCQMVLQESNVRREVLKARAMALKHLGKIFQREKDKQRGGTSKKTIGSHVSDDESSSDSSDDEEDSPRPLNYRTPMITQGIGR 391
Match: V4TD31 (J domain-containing protein {ECO:0000259|PROSITE:PS50076})
HSP 1 Score: 540.036 bits (1390), Expect = 0.000e+0
Identity = 278/378 (73.54%), Postives = 318/378 (84.13%), Query Frame = 0
Query: 1 MVKETEYYDVLGVIPAASEEEIRKAYYLKARQVHPDKNPNDPRAAERFQA-SEAYQVLSDPVQRDAYDRNGKYCISKDTMLDPTAVFALLFGSELFEDYIGHLAVASMASSELASESDNPERLNEKLKASKSIKLKDTQVYRRKAVQKDREDKLAGKLKNILQQYVRGDREGFQHFAETEAERLSHTAFGVNILHTIGYIYERQAAQELGKKAIYLGVPFMAEWVRNKGHFWKSQITAARGAFQLLQLQEDIRRQFKMDGSGPENDLESHLRLNKDTLMNSLWKLNVVDIEVTLLH----VILETGVKKEELKLRASALKLLGKIFQRDKYAQTSGTSKKKVA------ETSSDSSDDEDTPRALNYRAPLVTQKIDK 367
MVKETEYYD+LGV P+ASEEEIRKAYYLKARQVHPDKNPNDP+AAERFQ EAYQVLSDP QR+AYD+NGKY IS++TMLDPTAVFALLFGSELFEDYIGHLAVASMAS+ELA ESDNPE+LN+KLKAS VQ++RE++LA LK+ L QYVRGD++GF AE+E +R+S AFGV+ILHTIGYIY RQAAQELGKKA+YLGVPF+AEWVRNKGHFWKSQITAA+GAFQLLQLQEDIRRQFK+DGSGPEND+E+H+RLNK+TLMNSLWKLNVVDIEVTLLH V+ E V+KEELK RA ALK LGKIFQ DK + +GTS+KK A SSDSS ++D+PRAL+YR PL+TQ I +
Sbjct: 1 MVKETEYYDILGVNPSASEEEIRKAYYLKARQVHPDKNPNDPQAAERFQVLGEAYQVLSDPFQREAYDKNGKYSISRETMLDPTAVFALLFGSELFEDYIGHLAVASMASTELARESDNPEKLNDKLKAS--------------VVQREREERLARLLKDFLNQYVRGDKDGFLQRAESEGKRMSAVAFGVDILHTIGYIYSRQAAQELGKKALYLGVPFVAEWVRNKGHFWKSQITAAKGAFQLLQLQEDIRRQFKVDGSGPENDVETHIRLNKETLMNSLWKLNVVDIEVTLLHVCQMVLKENNVRKEELKARAVALKTLGKIFQ-DKQTENAGTSRKKNAADTDDDGNSSDSSSEDDSPRALSYRTPLLTQGIGR 363
Match: A0A067GXY2 (J domain-containing protein {ECO:0000259|PROSITE:PS50076})
HSP 1 Score: 540.036 bits (1390), Expect = 0.000e+0
Identity = 278/378 (73.54%), Postives = 318/378 (84.13%), Query Frame = 0
Query: 1 MVKETEYYDVLGVIPAASEEEIRKAYYLKARQVHPDKNPNDPRAAERFQA-SEAYQVLSDPVQRDAYDRNGKYCISKDTMLDPTAVFALLFGSELFEDYIGHLAVASMASSELASESDNPERLNEKLKASKSIKLKDTQVYRRKAVQKDREDKLAGKLKNILQQYVRGDREGFQHFAETEAERLSHTAFGVNILHTIGYIYERQAAQELGKKAIYLGVPFMAEWVRNKGHFWKSQITAARGAFQLLQLQEDIRRQFKMDGSGPENDLESHLRLNKDTLMNSLWKLNVVDIEVTLLH----VILETGVKKEELKLRASALKLLGKIFQRDKYAQTSGTSKKKVA------ETSSDSSDDEDTPRALNYRAPLVTQKIDK 367
MVKETEYYD+LGV P+ASEEEIRKAYYLKARQVHPDKNPNDP+AAERFQ EAYQVLSDP QR+AYD+NGKY IS++TMLDPTAVFALLFGSELFEDYIGHLAVASMAS+ELA ESDNPE+LN+KLKAS VQ++RE++LA LK+ L QYVRGD++GF AE+E +R+S AFGV+ILHTIGYIY RQAAQELGKKA+YLGVPF+AEWVRNKGHFWKSQITAA+GAFQLLQLQEDIRRQFK+DGSGPEND+E+H+RLNK+TLMNSLWKLNVVDIEVTLLH V+ E V+KEELK RA ALK LGKIFQ DK + +GTS+KK A SSDSS ++D+PRAL+YR PL+TQ I +
Sbjct: 1 MVKETEYYDILGVNPSASEEEIRKAYYLKARQVHPDKNPNDPQAAERFQVLGEAYQVLSDPFQREAYDKNGKYSISRETMLDPTAVFALLFGSELFEDYIGHLAVASMASTELARESDNPEKLNDKLKAS--------------VVQREREERLARLLKDFLNQYVRGDKDGFLQRAESEGKRMSAVAFGVDILHTIGYIYSRQAAQELGKKALYLGVPFVAEWVRNKGHFWKSQITAAKGAFQLLQLQEDIRRQFKVDGSGPENDVETHIRLNKETLMNSLWKLNVVDIEVTLLHVCQMVLKENNVRKEELKARAVALKTLGKIFQ-DKQTENAGTSRKKNAADTDDDGNSSDSSSEDDSPRALSYRTPLLTQGIGR 363
Match: A0A200Q562 ((DnaJ domain {ECO:0000313|EMBL:OVA05574.1}))
HSP 1 Score: 539.265 bits (1388), Expect = 0.000e+0
Identity = 274/379 (72.30%), Postives = 317/379 (83.64%), Query Frame = 0
Query: 1 MVKETEYYDVLGVIPAASEEEIRKAYYLKARQVHPDKNPNDPRAAERFQAS--EAYQVLSDPVQRDAYDRNGKYCISKDTMLDPTAVFALLFGSELFEDYIGHLAVASMASSELASESDNPERLNEKLKASKSIKLKDTQVYRRKAVQKDREDKLAGKLKNILQQYVRGDREGFQHFAETEAERLSHTAFGVNILHTIGYIYERQAAQELGKKAIYLGVPFMAEWVRNKGHFWKSQITAARGAFQLLQLQEDIRRQFKMDGSGPENDLESHLRLNKDTLMNSLWKLNVVDIEVTLLH----VILETGVKKEELKLRASALKLLGKIFQRDKYAQTSGTSKKK------VAETSSDSSDDEDTPRALNYRAPLVTQKIDK 367
MVKET+YYDVLGV P+ SEEEIRKAYYLKARQVHPDKNPNDP+AAERFQA+ EAYQVLSDPVQR+AYDRNGKYCIS++TMLDPTAVFALLFGSELFEDYIGH+AVASMASSELA E++ PE+L++++KA K AVQK+RE+KLA L++ L QYVRGD+ GF E+E +RLS TAFGV+ILHTIGYIYERQAAQELGKK IYLGVPF+AEWVRNKGHFWKSQITAA+GAFQLLQ+QED+RRQFKMDG+GPEN++ESH+ NKDTLMNSLWKLNVVDIEVTLLH V+ E VKKEEL+ RA ALK+LGKIFQR+K Q GTSK K +SSDSS ++D+P +L+YR PL+TQ I +
Sbjct: 1 MVKETQYYDVLGVSPSVSEEEIRKAYYLKARQVHPDKNPNDPQAAERFQANLREAYQVLSDPVQREAYDRNGKYCISRETMLDPTAVFALLFGSELFEDYIGHVAVASMASSELAGENEIPEKLHDRMKAIK-------------AVQKEREEKLASSLRDFLNQYVRGDKRGFVQRVESEVKRLSDTAFGVDILHTIGYIYERQAAQELGKKVIYLGVPFVAEWVRNKGHFWKSQITAAKGAFQLLQMQEDMRRQFKMDGTGPENNVESHILSNKDTLMNSLWKLNVVDIEVTLLHVCQMVLKENNVKKEELRARALALKILGKIFQREKNVQNGGTSKMKDVSNIDEDGSSSDSSSEKDSPHSLSYRTPLITQGIGR 366
| Match Name | Stats | Description |
|---|---|---|
| A0A1U8B2Y4 | E-Value: 0.000e+0, PID: 74.07 | (chaperone protein dnaJ 10 {ECO:0000313|RefSeq:XP_... [more] |
| A0A5J5B998 | E-Value: 0.000e+0, PID: 74.02 | J domain-containing protein {ECO:0000259|PROSITE:P... [more] |
| E0CQK2 | E-Value: 0.000e+0, PID: 74.35 | J domain-containing protein {ECO:0000259|PROSITE:P... [more] |
| A0A2G9H9D4 | E-Value: 0.000e+0, PID: 75.33 | (Molecular chaperone (DnaJ superfamily) {ECO:00003... [more] |
| A0A6I9T3D1 | E-Value: 0.000e+0, PID: 73.74 | (chaperone protein dnaJ 10 {ECO:0000313|RefSeq:XP_... [more] |
| A0A5N6RQ76 | E-Value: 0.000e+0, PID: 75.47 | J domain-containing protein {ECO:0000259|PROSITE:P... [more] |
| A0A6A4KUP0 | E-Value: 0.000e+0, PID: 71.18 | J domain-containing protein {ECO:0000259|PROSITE:P... [more] |
| V4TD31 | E-Value: 0.000e+0, PID: 73.54 | J domain-containing protein {ECO:0000259|PROSITE:P... [more] |
| A0A067GXY2 | E-Value: 0.000e+0, PID: 73.54 | J domain-containing protein {ECO:0000259|PROSITE:P... [more] |
| A0A200Q562 | E-Value: 0.000e+0, PID: 72.30 | (DnaJ domain {ECO:0000313|EMBL:OVA05574.1})[more] |
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An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions. | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |