MFSFVWWIIGFYWVSPDGQALIEQSPQLYWLCIIFLAFDVFFVVFCVALA
CIIGIAVCCCLPCIIAVLYAVVDQEGATKEDIEQLSKYKFRRLANTEKSN
GEIQVSFGGVMIECGTDSPTERILAAEDVDCCICLATYDDGAELRELPCA
HHFHCACIDKWLHINATCPLCKYDILKQSHTSIEEV*
| Relationships |
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| The polypeptide, DCAR_004666, derives from mRNA, DCAR_004666. |
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
Match: A0A2R6RCS6 ((E3 ubiquitin-protein like {ECO:0000313|EMBL:PSS26343.1}))
HSP 1 Score: 309.686 bits (792), Expect = 8.204e-104
Identity = 143/187 (76.47%), Postives = 159/187 (85.03%), Query Frame = 0
Query: 1 MFSFVWWIIGFYWVSPDGQALIEQSPQLYWLCIIFLAFDVFFVVFCVALACIIGIAVCCCLPCIIAVLYAVVDQEGATKEDIEQLSKYKFRRLANTEKSNGEIQVSFGGVMIECGTDSPTERILAAEDVDCCICLATYDDGAELRELPCAHHFHCACIDKWLHINATCPLCKYDILKQ-SHTSIEEV 186
MFSF+WWIIGFYWVS G+ L SPQLYWLCI FLAFDVFFVV CVA+ACIIG+AVCCCLPCIIA+LYAV DQEGATKEDI++L KYKFRRL EK NGEIQ SFGG+M EC TDSP E L+AED +CCICL TYDDG ELRELPC HHFHC+CIDKWL++NATCPLCK++ILK S +S EEV
Sbjct: 193 MFSFIWWIIGFYWVSAGGETLTRDSPQLYWLCITFLAFDVFFVVICVAVACIIGVAVCCCLPCIIAILYAVADQEGATKEDIDRLPKYKFRRLGEFEKQNGEIQESFGGIMTECDTDSPIEHALSAEDAECCICLCTYDDGTELRELPCRHHFHCSCIDKWLYMNATCPLCKFNILKNGSQSSSEEV 379
Match: A0A2R6QP61 ((E3 ubiquitin-protein like {ECO:0000313|EMBL:PSS11718.1}))
HSP 1 Score: 308.916 bits (790), Expect = 1.596e-103
Identity = 144/187 (77.01%), Postives = 159/187 (85.03%), Query Frame = 0
Query: 1 MFSFVWWIIGFYWVSPDGQALIEQSPQLYWLCIIFLAFDVFFVVFCVALACIIGIAVCCCLPCIIAVLYAVVDQEGATKEDIEQLSKYKFRRLANTEKSNGEIQVSFGGVMIECGTDSPTERILAAEDVDCCICLATYDDGAELRELPCAHHFHCACIDKWLHINATCPLCKYDILKQ-SHTSIEEV 186
MFSF+WWIIGFYWVS G+ L SPQLYWLCI FLAFDVFFVV CVA+ACIIGIAVCCCLPCIIA+LYAV DQEGATKEDI++L KYKFRRL EK NGEIQ SFGG+M EC TDSP E L+AED +CCICL TYDDG ELRELPC HHFHC+CIDKWL++NATCPLCK++ILK S +S EEV
Sbjct: 193 MFSFIWWIIGFYWVSAGGETLTHDSPQLYWLCITFLAFDVFFVVICVAVACIIGIAVCCCLPCIIAILYAVADQEGATKEDIDRLPKYKFRRLGEFEKQNGEIQESFGGLMTECDTDSPIEHALSAEDAECCICLCTYDDGTELRELPCRHHFHCSCIDKWLYMNATCPLCKFNILKNGSQSSSEEV 379
Match: A0A7J0EUZ0 ((Zinc finger, C3HC4 type (RING finger) family protein {ECO:0000313|EMBL:GFY90294.1}))
HSP 1 Score: 308.145 bits (788), Expect = 1.931e-103
Identity = 144/187 (77.01%), Postives = 159/187 (85.03%), Query Frame = 0
Query: 1 MFSFVWWIIGFYWVSPDGQALIEQSPQLYWLCIIFLAFDVFFVVFCVALACIIGIAVCCCLPCIIAVLYAVVDQEGATKEDIEQLSKYKFRRLANTEKSNGEIQVSFGGVMIECGTDSPTERILAAEDVDCCICLATYDDGAELRELPCAHHFHCACIDKWLHINATCPLCKYDILKQ-SHTSIEEV 186
MFSF+WWIIGFYWVS G+ L SPQLYWLCI FLAFDVFFVV CVA+ACIIGIAVCCCLPCIIA+LYAV DQEGATKEDI++L KYKFRRL EK NGEIQ SFGG+M EC TDSP E L+AED +CCICL TYDDG ELRELPC HHFHC+CIDKWL++NATCPLCK++ILK S +S EEV
Sbjct: 174 MFSFIWWIIGFYWVSAGGETLTHDSPQLYWLCITFLAFDVFFVVICVAVACIIGIAVCCCLPCIIAILYAVADQEGATKEDIDRLPKYKFRRLGEFEKQNGEIQESFGGLMTECDTDSPIEHALSAEDAECCICLCTYDDGTELRELPCRHHFHCSCIDKWLYMNATCPLCKFNILKNGSQSSSEEV 360
Match: A0A1U8BBD6 ((E3 ubiquitin-protein ligase At1g12760 isoform X2 {ECO:0000313|RefSeq:XP_010278591.1}))
HSP 1 Score: 308.145 bits (788), Expect = 4.611e-103
Identity = 140/188 (74.47%), Postives = 163/188 (86.70%), Query Frame = 0
Query: 1 MFSFVWWIIGFYWVSPDGQALIEQSPQLYWLCIIFLAFDVFFVVFCVALACIIGIAVCCCLPCIIAVLYAVVDQEGATKEDIEQLSKYKFRRLANTEKSNGEIQVSFGGVMIEC--GTDSPTERILAAEDVDCCICLATYDDGAELRELPCAHHFHCACIDKWLHINATCPLCKYDILKQSHTSIEEV 186
MFSF+WWIIGFYWVS GQALI SPQLYWLCIIFLAFDVFFV+FCVALAC+IGIAVCCCLPCIIAVLYAV DQEGA+KEDI+QL KYKF+R+ N EK GEIQ FGG+M +C T++P E +L+ ED +CCICL+TYDDGAELR+LPC+HHFHC+CIDKWL+INATCPLCKY+I++ + EEV
Sbjct: 199 MFSFIWWIIGFYWVSAGGQALIRDSPQLYWLCIIFLAFDVFFVIFCVALACVIGIAVCCCLPCIIAVLYAVADQEGASKEDIDQLPKYKFQRIGNFEKITGEIQGPFGGIMTKCVSDTETPIEHVLSLEDAECCICLSTYDDGAELRKLPCSHHFHCSCIDKWLYINATCPLCKYNIIRSGNQGREEV 386
Match: A0A1U8BB79 ((E3 ubiquitin-protein ligase At1g12760 isoform X1 {ECO:0000313|RefSeq:XP_010278589.1}))
HSP 1 Score: 308.145 bits (788), Expect = 4.863e-103
Identity = 140/188 (74.47%), Postives = 163/188 (86.70%), Query Frame = 0
Query: 1 MFSFVWWIIGFYWVSPDGQALIEQSPQLYWLCIIFLAFDVFFVVFCVALACIIGIAVCCCLPCIIAVLYAVVDQEGATKEDIEQLSKYKFRRLANTEKSNGEIQVSFGGVMIEC--GTDSPTERILAAEDVDCCICLATYDDGAELRELPCAHHFHCACIDKWLHINATCPLCKYDILKQSHTSIEEV 186
MFSF+WWIIGFYWVS GQALI SPQLYWLCIIFLAFDVFFV+FCVALAC+IGIAVCCCLPCIIAVLYAV DQEGA+KEDI+QL KYKF+R+ N EK GEIQ FGG+M +C T++P E +L+ ED +CCICL+TYDDGAELR+LPC+HHFHC+CIDKWL+INATCPLCKY+I++ + EEV
Sbjct: 200 MFSFIWWIIGFYWVSAGGQALIRDSPQLYWLCIIFLAFDVFFVIFCVALACVIGIAVCCCLPCIIAVLYAVADQEGASKEDIDQLPKYKFQRIGNFEKITGEIQGPFGGIMTKCVSDTETPIEHVLSLEDAECCICLSTYDDGAELRKLPCSHHFHCSCIDKWLYINATCPLCKYNIIRSGNQGREEV 387
Match: A0A7J0HEB5 ((Zinc finger, C3HC4 type (RING finger) family protein {ECO:0000313|EMBL:GFZ21064.1}))
HSP 1 Score: 306.99 bits (785), Expect = 1.149e-102
Identity = 142/187 (75.94%), Postives = 158/187 (84.49%), Query Frame = 0
Query: 1 MFSFVWWIIGFYWVSPDGQALIEQSPQLYWLCIIFLAFDVFFVVFCVALACIIGIAVCCCLPCIIAVLYAVVDQEGATKEDIEQLSKYKFRRLANTEKSNGEIQVSFGGVMIECGTDSPTERILAAEDVDCCICLATYDDGAELRELPCAHHFHCACIDKWLHINATCPLCKYDILKQ-SHTSIEEV 186
MFSF+WWIIGFYWVS + L SPQLYWLCI FLAFDVFFVV CVA+ACIIG+AVCCCLPCIIA+LYAV DQEGATKEDI++L KYKFRRL EK NGEIQ SFGG+M EC TDSP E L+AED +CCICL TYDDG ELRELPC HHFHC+CIDKWL++NATCPLCK++ILK S +S EEV
Sbjct: 193 MFSFIWWIIGFYWVSAGDETLTRDSPQLYWLCITFLAFDVFFVVICVAVACIIGVAVCCCLPCIIAILYAVADQEGATKEDIDRLPKYKFRRLGEFEKQNGEIQESFGGIMTECDTDSPIEHALSAEDAECCICLCTYDDGTELRELPCRHHFHCSCIDKWLYMNATCPLCKFNILKNGSQSSSEEV 379
Match: A0A7J7KUD3 (RING-type domain-containing protein {ECO:0000259|PROSITE:PS50089})
HSP 1 Score: 305.449 bits (781), Expect = 2.083e-102
Identity = 136/185 (73.51%), Postives = 159/185 (85.95%), Query Frame = 0
Query: 1 MFSFVWWIIGFYWVSPDGQALIEQSPQLYWLCIIFLAFDVFFVVFCVALACIIGIAVCCCLPCIIAVLYAVVDQEGATKEDIEQLSKYKFRRLANTEKSNGEIQVSFGGVMIECGTDSPTERILAAEDVDCCICLATYDDGAELRELPCAHHFHCACIDKWLHINATCPLCKYDILKQSHTSIEE 185
MFSF+WWIIGFYWVS GQ+ + SP+LYWLCIIFLAFDV FVVFCVALACIIGIAVCCCLPCIIA+LYAV +QEGA+KE+I+QL K+KFRR+ EK NGEIQ FGG+M EC TD P E++L+ ED +CCICL++YDDGAELRELPC HHFHCACIDKWLH+NATCPLCKY+I+K ++ E
Sbjct: 175 MFSFIWWIIGFYWVSAGGQSYTQDSPKLYWLCIIFLAFDVIFVVFCVALACIIGIAVCCCLPCIIAILYAVAEQEGASKEEIDQLPKFKFRRICEMEKLNGEIQGPFGGIMTECNTDPPVEQVLSPEDAECCICLSSYDDGAELRELPCGHHFHCACIDKWLHLNATCPLCKYNIVKSNNNRGRE 359
Match: A0A7J7C773 ((E3 ubiquitin-protein ligase {ECO:0000313|EMBL:KAF5729962.1}))
HSP 1 Score: 306.605 bits (784), Expect = 2.719e-102
Identity = 139/186 (74.73%), Postives = 157/186 (84.41%), Query Frame = 0
Query: 1 MFSFVWWIIGFYWVSPDGQALIEQSPQLYWLCIIFLAFDVFFVVFCVALACIIGIAVCCCLPCIIAVLYAVVDQEGATKEDIEQLSKYKFRRLANTEKSNGEIQVSFGGVMIECGTDSPTERILAAEDVDCCICLATYDDGAELRELPCAHHFHCACIDKWLHINATCPLCKYDILKQSHTSIEEV 186
MFSF+WWIIGFYWVS GQ L +SPQLYWLCIIFL DVFFVVFCVALAC IGIAVCCCLPCIIA+LYAV DQEGA+KEDI+ LSKYKFRR+ + EK G+ + S GG+M ECGTD+P ER L+ EDV+CCICL+ YDDG ELRELPC HHFHCAC+DKWL++NATCPLCKY+ILK S EEV
Sbjct: 211 MFSFIWWIIGFYWVSAGGQTLARESPQLYWLCIIFLGLDVFFVVFCVALACFIGIAVCCCLPCIIAILYAVADQEGASKEDIDMLSKYKFRRVGDDEKVTGDGRESIGGIMTECGTDAPFERSLSPEDVECCICLSAYDDGIELRELPCHHHFHCACVDKWLYLNATCPLCKYNILKSSSQDREEV 396
Match: A0A1U8PZB8 ((E3 ubiquitin-protein ligase At4g11680-like {ECO:0000313|RefSeq:XP_016755708.1}))
HSP 1 Score: 305.449 bits (781), Expect = 4.102e-102
Identity = 139/186 (74.73%), Postives = 162/186 (87.10%), Query Frame = 0
Query: 1 MFSFVWWIIGFYWVSPDGQALIEQSPQLYWLCIIFLAFDVFFVVFCVALACIIGIAVCCCLPCIIAVLYAVVDQEGATKEDIEQLSKYKFRRLANTEKSNGEIQVSFGGVMIECGTDSPTERILAAEDVDCCICLATYDDGAELRELPCAHHFHCACIDKWLHINATCPLCKYDILKQSHTSIEEV 186
MFSFVWWI GFYWVS GQAL SPQLYWLCIIFLAFDVFFVVFC+ALACIIGIAVCCCLPCIIA+LYAV DQEGA+KE+I+QLSK+KF+++ + EK G++Q GG+MIECGTDSP E++L+ +D +CCICL+ Y+DG ELRELPC HHFHCAC+DKWL+INATCPLCKY+ILK S S EEV
Sbjct: 199 MFSFVWWITGFYWVSLGGQALARGSPQLYWLCIIFLAFDVFFVVFCIALACIIGIAVCCCLPCIIAILYAVADQEGASKEEIDQLSKFKFQKIGDNEKPAGDVQGPVGGIMIECGTDSPMEQVLSQDDAECCICLSAYEDGVELRELPCRHHFHCACVDKWLYINATCPLCKYNILKSS--SQEEV 382
Match: A0A2G9HWV5 (RING-type domain-containing protein {ECO:0000259|PROSITE:PS50089})
HSP 1 Score: 305.064 bits (780), Expect = 7.312e-102
Identity = 138/186 (74.19%), Postives = 157/186 (84.41%), Query Frame = 0
Query: 1 MFSFVWWIIGFYWVSPDGQALIEQSPQLYWLCIIFLAFDVFFVVFCVALACIIGIAVCCCLPCIIAVLYAVVDQEGATKEDIEQLSKYKFRRLANTEKSNGEIQVSFGGVMIECGTDSPTERILAAEDVDCCICLATYDDGAELRELPCAHHFHCACIDKWLHINATCPLCKYDILKQSHTSIEEV 186
MFSF+WWIIGFYWVS GQ L +SPQLYWLCI FLAFDVFFVV CVA+A +IGIAVCCCLPCIIA+LYAV DQEGATKEDIE+L +YKF+++ + K NGEIQ SFGG+M +C TDSPTE +L ED +CCICL+ YDDG ELRELPC HHFH ACIDKWLHINATCPLCK++ILK + S EEV
Sbjct: 197 MFSFIWWIIGFYWVSAGGQDLPHESPQLYWLCITFLAFDVFFVVICVAVASVIGIAVCCCLPCIIAILYAVADQEGATKEDIERLPRYKFQKIGDFGKRNGEIQESFGGIMTKCDTDSPTEHVLPLEDAECCICLSAYDDGTELRELPCRHHFHSACIDKWLHINATCPLCKFNILKNGNQSSEEV 382
| Match Name | Stats | Description |
|---|---|---|
| A0A2R6RCS6 | E-Value: 8.204e-104, PID: 76.47 | (E3 ubiquitin-protein like {ECO:0000313|EMBL:PSS26... [more] |
| A0A2R6QP61 | E-Value: 1.596e-103, PID: 77.01 | (E3 ubiquitin-protein like {ECO:0000313|EMBL:PSS11... [more] |
| A0A7J0EUZ0 | E-Value: 1.931e-103, PID: 77.01 | (Zinc finger, C3HC4 type (RING finger) family prot... [more] |
| A0A1U8BBD6 | E-Value: 4.611e-103, PID: 74.47 | (E3 ubiquitin-protein ligase At1g12760 isoform X2 ... [more] |
| A0A1U8BB79 | E-Value: 4.863e-103, PID: 74.47 | (E3 ubiquitin-protein ligase At1g12760 isoform X1 ... [more] |
| A0A7J0HEB5 | E-Value: 1.149e-102, PID: 75.94 | (Zinc finger, C3HC4 type (RING finger) family prot... [more] |
| A0A7J7KUD3 | E-Value: 2.083e-102, PID: 73.51 | RING-type domain-containing protein {ECO:0000259|P... [more] |
| A0A7J7C773 | E-Value: 2.719e-102, PID: 74.73 | (E3 ubiquitin-protein ligase {ECO:0000313|EMBL:KAF... [more] |
| A0A1U8PZB8 | E-Value: 4.102e-102, PID: 74.73 | (E3 ubiquitin-protein ligase At4g11680-like {ECO:0... [more] |
| A0A2G9HWV5 | E-Value: 7.312e-102, PID: 74.19 | RING-type domain-containing protein {ECO:0000259|P... [more] |
Pages
back to top| Name | Description |
|---|---|
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions. | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |