DCAR_000702

Resource Type: 
Polypeptide
Name: 
DCAR_000702
Identifier: 
DCAR_000702.mRNA-protein
Sequence: 
MKRWGETITVVVLAVTMVIESAMSDPQANKISKTGRESSGYEVRSFFTEI
ELGTSSEVCADNKTVPAQAAFTLVAVQLLKDLADATPKIKNFFGAAKRQV
FDGGPSAKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSS
GGGSSASGGCFLRYSVTPFFSDNDITNIMPYLQKGSSSKKTAIIAGGVGA
AWKSYNNGTHEDLIDETIDSSEYNVENAKKMIEIALKCTQSPVSLRPTMS
EVVVMLVNDASPEPKQTN*
Sequence Length: 
269
Sequence Checksum: 
39f31232316a247688d60e718ffac731
View location in JBrowse: 
Relationship: 
There is 1 relationship.
Relationships
The polypeptide, DCAR_000702, derives from mRNA, DCAR_000702.
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Blast Results: 
The following BLAST results are available for this feature:
BLAST of DCAR_000702 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
BLAST of DCAR_000702 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2Z6NPU8 ((Uncharacterized protein {ECO:0000313|EMBL:GAU44113.1}))

HSP 1 Score: 152.91 bits (385), Expect = 4.856e-41
Identity = 96/261 (36.78%), Postives = 126/261 (48.28%), Query Frame = 0
 
Query:   41 YEVRSFFTEIELGTSSEVCADNKTVPAQAAFTLVAVQLLKDLADATPKIKNFFGAAKRQVFDGGPSAKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSASGGCFLRYSVTPFFSDNDITNIMPYLQKGSSSKKTA-------------------------------------IIAGGVGA--------AWKSYNNGTHEDLIDETIDSSEYNVENAKKMIEIALKCTQSPVSLRPTMSEVVVML 256
            YE   FF +  L  +S +C   KT      F   A Q+L DL  ATPKI  FF A K QV        A   +YA+AQC  TV++  C  CL  G++NIQ C   + G +        GCF+RYS   FF+DN   +I P+L++GSSSKK A                                     +I G  G         AWK Y    H +L+D+T++ S+Y+ E  KK+I IAL CTQ+  S RPTMSE+VV+L
Sbjct:  128 YESNGFFDQTTLPGNSVLCG-TKTANGATTFNAAAQQILLDLQTATPKIHGFFAATKTQV--------AGNAIYAIAQCAETVTESGCLDCLTVGFTNIQGCFPNTDGEA-----FDAGCFMRYSEKSFFADNQTIDITPFLKQGSSSKKGAIIGGVVGGVVLVLILLALFVWFRLKKKPKRHPRAVKKLILGHSGKMDEQFESEAWKLYEVDRHLELVDKTLNPSDYDAEEVKKIIAIALLCTQATASTRPTMSEIVVLL 374    

HSP 2 Score: 46.9802 bits (110), Expect = 1.842e-3
Identity = 27/70 (38.57%), Postives = 33/70 (47.14%), Query Frame = 0
 
Query:  101 FDGGPSAKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSASGGCFLRYSVTPFF 170
            F    SAK +  VYA+ QC   +S  DC  C     + I+ C   S GS+ G      GCFLRY    FF
Sbjct:   69 FATAQSAKGANPVYALFQCRNYLSNSDCSACFAVAAAQIRNC---SAGSN-GARVIYDGCFLRYESNGFF 134    
BLAST of DCAR_000702 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A103Y3T3 ((Gnk2-homologous domain-containing protein {ECO:0000313|EMBL:KVI01990.1}))

HSP 1 Score: 122.479 bits (306), Expect = 8.151e-30
Identity = 86/235 (36.60%), Postives = 120/235 (51.06%), Query Frame = 0
 
Query:   41 YEVRSFFTEIELGTSSEVCADNKTVPAQAAFTLVAVQLLKDLADATPKIKNFFGAAKRQVFDGGPSAKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSASGGCFLRYSVTPFFSDN-------DITNI-MPYLQKGSSSKKTAIIAGGVG------AAWKSYNNGTHEDLIDETIDSSEYNVENAKKMIEIALKCTQSPVSLRPTMSEVVVMLVNDAS 261
            YE  SF+ E     +  +C  N+T   Q  F      LL DL+ ATP+I  F+ AA   V  G  + K    VYA+AQC+  V++  C  CL   Y+NI+ C +        G +   GCF+R    P ++ +       D  +  +  L+  S  + T +     G       AW  Y    H  L+DET+D S+Y  ++ KK+IEIAL CTQSPVS+RPTMSE VVML++D S
Sbjct:  125 YESNSFYDETTNDGNVGLCG-NRTSSRQGVFETAVDGLLSDLSIATPRISGFYAAATAPVV-GTNTTK----VYAIAQCVERVTRNGCKDCLHVAYANIKICPT----VVTDGRAIDSGCFMRGYTAPEYAIHGQLSEKVDTYSFGIVVLEIVSGKRCTDVRNESAGEQYLLEHAWNLYETRMHLKLVDETLDPSKYREDDIKKVIEIALMCTQSPVSVRPTMSE-VVMLLSDRS 348    

HSP 2 Score: 41.2022 bits (95), Expect = 1.291e-1
Identity = 23/86 (26.74%), Postives = 35/86 (40.70%), Query Frame = 0
 
Query:   93 FGAAKRQV------FDGGPSAKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSASGGCFLRYSVTPFFSD 172
            FG  +RQ+      F      + S  VY + QC   +S  DC  C      +I++C       + G      GC+LRY    F+ +
Sbjct:   53 FGNVRRQLSNNKTYFVTAEQTRNSEPVYVLCQCRKYMSTRDCVACFDFAVRSIRSC-----APAKGARRVLDGCYLRYESNSFYDE 133    
BLAST of DCAR_000702 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A067LER0 ((Uncharacterized protein {ECO:0000313|EMBL:KDP46966.1}))

HSP 1 Score: 123.635 bits (309), Expect = 2.269e-29
Identity = 65/159 (40.88%), Postives = 90/159 (56.60%), Query Frame = 0
 
Query:   41 YEVRSFFTEIELGTSSEVCADNKTVPAQAAFTLVAVQLLKDLADATPKIKNFFGAAKRQVFDGGPSAKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSASGGCFLRYSVTPFFSDNDITNIMPYLQKGSSSKKTAIIAGGVG 199
            YE   F+ E     + E C +N+T    AAF   A  LL+DL  ATP+I  FF A+KR +        ++ +VY +AQC+  +    C  C+   Y+NIQ CL ++ G +        GCF+RYS  PFF+DN   N+ P+L+ GSSSKKTA+IAG  G
Sbjct:  109 YERNDFYGETTRDANREYC-ENQTTSLTAAFQTTADSLLRDLQIATPRINGFFTASKRDIV-----GVSNASVYGIAQCVQIIDMSGCLACMQVAYANIQRCLPKTDGRA-----VDSGCFMRYSNKPFFADNQTINLEPFLKTGSSSKKTALIAGVAG 256    

HSP 2 Score: 81.6481 bits (200), Expect = 7.232e-15
Identity = 37/65 (56.92%), Postives = 47/65 (72.31%), Query Frame = 0
 
Query:  201 AWKSYNNGTHEDLIDETIDSSEYNVENAKKMIEIALKCTQSPVSLRPTMSEVVVMLVNDASPEPK 265
            AWK Y NGTH +L+DE++   EY  E  KK+IEIAL CTQ+  +LRPTMSEVVV+L +  S E +
Sbjct:  524 AWKLYENGTHLELVDESLGPDEYEAEEVKKIIEIALMCTQASHALRPTMSEVVVLLKSTGSLEHR 588    

HSP 3 Score: 37.3502 bits (85), Expect = 3.364e+0
Identity = 17/60 (28.33%), Postives = 26/60 (43.33%), Query Frame = 0
 
Query:  113 VYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSASGGCFLRYSVTPFFSD 172
            V+A+ QC   +S  +C  C       +++C         GG     GCFLRY    F+ +
Sbjct:   63 VFAMVQCRDYMSTAECIACFRAASIEVRSC-----SPGNGGRVVYDGCFLRYERNDFYGE 117    
BLAST of DCAR_000702 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A1S3VJC3 ((cold-responsive protein kinase 1-like {ECO:0000313|RefSeq:XP_014518199.2}))

HSP 1 Score: 123.25 bits (308), Expect = 3.173e-29
Identity = 69/175 (39.43%), Postives = 97/175 (55.43%), Query Frame = 0
 
Query:   41 YEVRSFFTEI-ELGTSSEVCADNKTVPAQAAFTLVAVQLLKDLADATPKIKNFFGAAKRQVFDGGPSAKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSASGGCFLRYSVTPFFSDNDITNIMPYLQKGSSSKKTAIIAGGVGA---------AWKSY 205
            YE + FF +  ELG    +C  N +      F LV  Q++ D+  ATPK ++F+ A K QV +G         +YAVAQC+ T ++  C  C+  GY+N+Q+CL  + G +        GCF+RYS TPFF+DN   +I PYL+KG SS+K  IIA  VGA         AW+ +
Sbjct:  125 YESQMFFDQTNELGKGV-LCGKNTS--NATGFGLVGRQVIADIQTATPKTRDFYAATKTQVANG-------TAIYAVAQCVETATEDKCLSCMQAGYNNLQSCLPNTEGRA-----YDAGCFMRYSTTPFFADNQTIDIAPYLKKGDSSRKWIIIAASVGAVVALLCVLFAWRWF 284    

HSP 2 Score: 73.559 bits (179), Expect = 3.819e-12
Identity = 31/55 (56.36%), Postives = 42/55 (76.36%), Query Frame = 0
 
Query:  202 WKSYNNGTHEDLIDETIDSSEYNVENAKKMIEIALKCTQSPVSLRPTMSEVVVML 256
            WK Y  G H +L+D+ ID ++Y+ E+ KK+IEIAL CTQ+  + RPTMSEVVV+L
Sbjct:  537 WKLYERGMHLELVDKAIDPNDYDAEDVKKIIEIALLCTQASAATRPTMSEVVVLL 591    

HSP 3 Score: 41.2022 bits (95), Expect = 1.505e-1
Identity = 24/84 (28.57%), Postives = 33/84 (39.29%), Query Frame = 0
 
Query:   93 FGAAKRQV------FDGGPSAKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSASGGCFLRYSVTPFF 170
            F   +R++      F     A   V  YA+ QC   +S+ DC  C     + I+ C       + G      GCFLRY    FF
Sbjct:   53 FSNLRREISNNSKHFGTTQQAIGDVLTYAMFQCRNYLSKNDCLSCFNTASTQIRNC-----SQANGARVIYDGCFLRYESQMFF 131    
BLAST of DCAR_000702 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A0S3S5N9 ((Uncharacterized protein {ECO:0000313|EMBL:BAT88156.1}))

HSP 1 Score: 123.25 bits (308), Expect = 3.298e-29
Identity = 64/159 (40.25%), Postives = 90/159 (56.60%), Query Frame = 0
 
Query:   41 YEVRSFFTEIELGTSSEVCADNKTVPAQAAFTLVAVQLLKDLADATPKIKNFFGAAKRQVFDGGPSAKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSASGGCFLRYSVTPFFSDNDITNIMPYLQKGSSSKKTAIIAGGVG 199
            YE   FF + +      +C  N +    A F LV  Q++ D+  ATPK ++F+ A K QV +G         +YAVAQC+ T ++  C  C+  GY+N+Q+CL  + G +        GCF+RYS TPFF+DN   +I PYL+KG SS+K  IIA  VG
Sbjct:  128 YESEMFFDQTDELGKGVLCGKNTS--NAAGFGLVGQQVIADIQTATPKTRDFYAATKTQVANG-------TAIYAVAQCVETATEDKCLSCMQAGYNNLQSCLPNTEGRA-----YDAGCFMRYSTTPFFADNQTIDIAPYLKKGDSSRKWIIIAASVG 272    

HSP 2 Score: 73.559 bits (179), Expect = 3.584e-12
Identity = 31/55 (56.36%), Postives = 42/55 (76.36%), Query Frame = 0
 
Query:  202 WKSYNNGTHEDLIDETIDSSEYNVENAKKMIEIALKCTQSPVSLRPTMSEVVVML 256
            WK Y  G H +L+D+ ID ++Y+ E+ KK+IEIAL CTQ+  + RPTMSEVVV+L
Sbjct:  540 WKLYERGMHLELVDKAIDPNDYDAEDVKKIIEIALLCTQASAATRPTMSEVVVLL 594    

HSP 3 Score: 45.4394 bits (106), Expect = 7.597e-3
Identity = 23/74 (31.08%), Postives = 30/74 (40.54%), Query Frame = 0
 
Query:  101 FDGGPSAKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSASGGCFLRYSVTPFFSDND 174
            F     A+  V  YA+ QC   +S+ DC  C     + I+ C       + G      GCFLRY    FF   D
Sbjct:   70 FGTTQEAREDVLTYAMFQCRNYLSKNDCLSCFNNASTQIRNC-----SQANGARVIYDGCFLRYESEMFFDQTD 138    
BLAST of DCAR_000702 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A0L9VCJ6 ((Uncharacterized protein {ECO:0000313|EMBL:KOM52786.1}))

HSP 1 Score: 123.25 bits (308), Expect = 3.531e-29
Identity = 64/159 (40.25%), Postives = 90/159 (56.60%), Query Frame = 0
 
Query:   41 YEVRSFFTEIELGTSSEVCADNKTVPAQAAFTLVAVQLLKDLADATPKIKNFFGAAKRQVFDGGPSAKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSASGGCFLRYSVTPFFSDNDITNIMPYLQKGSSSKKTAIIAGGVG 199
            YE   FF + +      +C  N +    A F LV  Q++ D+  ATPK ++F+ A K QV +G         +YAVAQC+ T ++  C  C+  GY+N+Q+CL  + G +        GCF+RYS TPFF+DN   +I PYL+KG SS+K  IIA  VG
Sbjct:  149 YESEMFFDQTDELGKGVLCGKNTS--NAAGFGLVGQQVIADIQTATPKTRDFYAATKTQVANG-------TAIYAVAQCVETATEDKCLSCMQAGYNNLQSCLPNTEGRA-----YDAGCFMRYSTTPFFADNQTIDIAPYLKKGDSSRKWIIIAASVG 293    

HSP 2 Score: 73.559 bits (179), Expect = 4.020e-12
Identity = 31/55 (56.36%), Postives = 42/55 (76.36%), Query Frame = 0
 
Query:  202 WKSYNNGTHEDLIDETIDSSEYNVENAKKMIEIALKCTQSPVSLRPTMSEVVVML 256
            WK Y  G H +L+D+ ID ++Y+ E+ KK+IEIAL CTQ+  + RPTMSEVVV+L
Sbjct:  561 WKLYERGMHLELVDKAIDPNDYDAEDVKKIIEIALLCTQASAATRPTMSEVVVLL 615    

HSP 3 Score: 36.1946 bits (82), Expect = 7.815e+0
Identity = 23/79 (29.11%), Postives = 31/79 (39.24%), Query Frame = 0
 
Query:  107 AKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSASGGCFLRYSVTPFFSDN----DITNIMPY 181
            A+  V  YA+ QC   +S+ DC  C     + I+ C       + G      GCFL  S     S N       N+M Y
Sbjct:   76 AREDVLTYAMFQCRNYLSKNDCLSCFNNASTQIRNC-----SQANGARVIYDGCFLSVSYVEDLSINFNVGYYENVMRY 149    
BLAST of DCAR_000702 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A371GYB0 ((Cysteine-rich receptor-like protein kinase 2 {ECO:0000313|EMBL:RDX95534.1}) (Fragment))

HSP 1 Score: 122.094 bits (305), Expect = 1.032e-28
Identity = 70/172 (40.70%), Postives = 95/172 (55.23%), Query Frame = 0
 
Query:   41 YEVRSFFTEI-ELGTSSEVCADNKTVPAQAAFTLVAVQLLKDLADATPKIKNFFGAAKRQVFDGGPSAKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSASGGCFLRYSVTPFFSDNDITNIMPYLQKGSSSKKTAIIAGGVGA--------AWK 203
            YE   F+ +  ELG S  +C +  T+ +   F     Q L DL  ATPK K+F+ A K QV  G         +YA+AQC+ T ++ +C  C+  GY+N+Q+CL  + G S        GC++RYS TPFF+DN   +I PY + G SSKK AII GGVGA        AW+
Sbjct:  126 YESERFYNQTNELG-SGVLCGN--TISSVTGFGEAGQQALMDLQIATPKTKDFYAATKTQVVGGS-------AIYAIAQCVETATETNCLNCMQVGYNNLQSCLPYTEGRS-----YDAGCYMRYSTTPFFADNQTIDITPYFKHGDSSKKWAIIGGGVGAVVLILALFAWR 282    

HSP 2 Score: 114.39 bits (285), Expect = 5.341e-26
Identity = 59/129 (45.74%), Postives = 75/129 (58.14%), Query Frame = 0
 
Query:   71 FTLVAVQLLKDLADATPKIKNFFGAAKRQVFDGGPSAKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSASGGCFLRYSVTPFFSDNDITNIMPYLQKGSSSKKTAIIAGGVG 199
            F  V  Q L DL  ATPKIK F+ A K QV  G         +YA+AQC+ T S+ +C  CL  GY+N+Q+CL  + G +        GCF+RYS  PFF+DN   +I PYL+ G SSKK  II G +G
Sbjct:  718 FKAVGQQALMDLQIATPKIKGFYAATKTQVVAGS-------AIYAIAQCVETASEPNCLNCLQLGYNNLQSCLPNTEGRA-----YDAGCFMRYSTKPFFADNQTIDIAPYLKHGDSSKKWPIIGGVIG 834    

HSP 3 Score: 72.7886 bits (177), Expect = 7.814e-12
Identity = 31/55 (56.36%), Postives = 41/55 (74.55%), Query Frame = 0
 
Query:  202 WKSYNNGTHEDLIDETIDSSEYNVENAKKMIEIALKCTQSPVSLRPTMSEVVVML 256
            WK Y  G H +L+D+ ID ++Y+ E  KK+IEIAL CTQ+  + RPTMSEVVV+L
Sbjct: 1084 WKLYERGMHLELVDKAIDPNDYDAEEVKKIIEIALLCTQASAATRPTMSEVVVLL 1138    

HSP 4 Score: 70.8626 bits (172), Expect = 3.067e-11
Identity = 31/56 (55.36%), Postives = 39/56 (69.64%), Query Frame = 0
 
Query:  201 AWKSYNNGTHEDLIDETIDSSEYNVENAKKMIEIALKCTQSPVSLRPTMSEVVVML 256
            +WK +  G H +L+D+ ID +EY  E  KK+IEIAL CTQ   S RPTMSEVV +L
Sbjct:  535 SWKLHERGLHMELVDKAIDPNEYEAEEVKKIIEIALSCTQPSASTRPTMSEVVALL 590    

HSP 5 Score: 41.2022 bits (95), Expect = 1.834e-1
Identity = 21/68 (30.88%), Postives = 29/68 (42.65%), Query Frame = 0
 
Query:  107 AKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSASGGCFLRYSVTPFFSDND 174
            A+     YA+ QC    S+ DC  CL    + IQ C       + G      GCFLRY    F++  +
Sbjct:   74 ARGDALTYAMFQCRNYQSKNDCLVCLNTAITQIQNC-----SKANGARVIYDGCFLRYESERFYNQTN 136    

HSP 6 Score: 37.7354 bits (86), Expect = 2.496e+0
Identity = 22/65 (33.85%), Postives = 29/65 (44.62%), Query Frame = 0
 
Query:  107 AKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSA-SGGCFLRYSVTPFF 170
            A+  V  YA+ QC   +S  DC  C       I+ C      S+G G+     GCFLRY    F+
Sbjct:  638 ARGEVLTYAMFQCRNYLSIKDCLACFDVASIQIRNC------STGNGARVIYDGCFLRYESERFY 696    
BLAST of DCAR_000702 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2N9GWR1 ((Uncharacterized protein {ECO:0000313|EMBL:SPD06787.1}))

HSP 1 Score: 119.398 bits (298), Expect = 1.325e-28
Identity = 71/154 (46.10%), Postives = 87/154 (56.49%), Query Frame = 0
 
Query:   41 YEVRSFFTEIELGTSSEVCADNKTVPAQAAFTLVAVQLLKDLADATPKIKNFFGAAKRQVFDGGPSAKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSASGGCFLRYSVTPFFSDNDITNIMPYLQKGSSSKKTAII 194
            YE  SF+ +  L  S  +C  N+T     AF+    +LL DL  ATPKI  FF A+K++V  GG     +VTVYAVAQC+ T S+  C  CL    SN Q CL      S  G +   GCFLRYS TPFF+DN   +I  +L  G SSKK AII
Sbjct:  124 YESASFYDQTTLPGSEGICG-NQTTSQPTAFSTTGEELLNDLQTATPKINGFFAASKKEVVVGG-----NVTVYAVAQCVETASESGCQDCLKIASSNAQRCLP-----SADGRAYDAGCFLRYSDTPFFADNQTIDINQFLGNGGSSKKKAII 266    

HSP 2 Score: 43.5134 bits (101), Expect = 3.021e-2
Identity = 39/165 (23.64%), Postives = 65/165 (39.39%), Query Frame = 0
 
Query:   11 VVLAVTMV-----IESAMSDPQANKISKTGRESSGYEVRSFFTEIELGTSSEVCADNKTVPAQAAFTLVAVQLLKDLADATPKIKNFFGAAKRQVFDGGPSAKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSASGGCFLRYSVTPFF 170
            +VL VT +     +   ++DPQ N ++    + +  +V +F+T +                  A F+ +  QL  +         N + A  +QV    P+       YA+ QC   +S+ DC  C     S I+ C      ++ G      GCFLRY    F+
Sbjct:    4 MVLRVTFIWWLWGLRLGVADPQINLLNSGCSQYNVSDVSNFYTNL-----------------NATFSDLRTQLNNN---------NKYFATAQQVIGSEPA-------YAMVQCRNYLSKSDCIACFTTAVSQIRNC-----SAANGARVIYDGCFLRYESASFY 130    
BLAST of DCAR_000702 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A445I578 ((Cysteine-rich receptor-like protein kinase 2 {ECO:0000313|EMBL:RZB81152.1}))

HSP 1 Score: 121.324 bits (303), Expect = 1.712e-28
Identity = 71/155 (45.81%), Postives = 89/155 (57.42%), Query Frame = 0
 
Query:   41 YEVRSFFTEI-ELGTSSEVCADNKTVPAQAAFTLVAVQLLKDLADATPKIKNFFGAAKRQVFDGGPSAKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSASGGCFLRYSVTPFFSDNDITNIMPYLQKGSSSKKTAII 194
            YE   F+ +  E+G    V   NK+  A   F  V  Q L DL  ATPKIK F+ A K QV      A  S  +YA+AQC+ T SQ  C  C+  GY+N+Q+CL      S  GS+   GCF+R+S TPFF+DN   NI PYL++G SSKK AII
Sbjct:  131 YESERFYQQTNEIGGG--VTCGNKSTNA-TGFRAVGQQALLDLQKATPKIKGFYAATKTQV------AGGSANIYAIAQCVETASQQKCLDCMQVGYNNLQSCLP-----STDGSAYDAGCFMRFSTTPFFADNQTINIRPYLKEGGSSKKWAII 271    

HSP 2 Score: 71.2478 bits (173), Expect = 2.164e-11
Identity = 31/56 (55.36%), Postives = 41/56 (73.21%), Query Frame = 0
 
Query:  201 AWKSYNNGTHEDLIDETIDSSEYNVENAKKMIEIALKCTQSPVSLRPTMSEVVVML 256
            AWK Y  G   +L+D+ ID +EY+ E  KK+IEIAL CTQ+  + RPTMSE+VV+L
Sbjct:  542 AWKLYERGMQLELVDKDIDPNEYDAEEVKKIIEIALLCTQASAATRPTMSELVVLL 597    
BLAST of DCAR_000702 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: I1LM52 ((Uncharacterized protein {ECO:0000313|EMBL:KRH30805.1, ECO:0000313|EnsemblPlants:KRH30805}))

HSP 1 Score: 121.324 bits (303), Expect = 1.712e-28
Identity = 71/155 (45.81%), Postives = 89/155 (57.42%), Query Frame = 0
 
Query:   41 YEVRSFFTEI-ELGTSSEVCADNKTVPAQAAFTLVAVQLLKDLADATPKIKNFFGAAKRQVFDGGPSAKASVTVYAVAQCLGTVSQGDCGKCLMGGYSNIQTCLSQSGGSSGGGSSASGGCFLRYSVTPFFSDNDITNIMPYLQKGSSSKKTAII 194
            YE   F+ +  E+G    V   NK+  A   F  V  Q L DL  ATPKIK F+ A K QV      A  S  +YA+AQC+ T SQ  C  C+  GY+N+Q+CL      S  GS+   GCF+R+S TPFF+DN   NI PYL++G SSKK AII
Sbjct:  131 YESERFYQQTNEIGGG--VTCGNKSTNA-TGFRAVGQQALLDLQKATPKIKGFYAATKTQV------AGGSANIYAIAQCVETASQQKCLDCMQVGYNNLQSCLP-----STDGSAYDAGCFMRFSTTPFFADNQTINIRPYLKEGGSSKKWAII 271    

HSP 2 Score: 71.2478 bits (173), Expect = 2.164e-11
Identity = 31/56 (55.36%), Postives = 41/56 (73.21%), Query Frame = 0
 
Query:  201 AWKSYNNGTHEDLIDETIDSSEYNVENAKKMIEIALKCTQSPVSLRPTMSEVVVML 256
            AWK Y  G   +L+D+ ID +EY+ E  KK+IEIAL CTQ+  + RPTMSE+VV+L
Sbjct:  542 AWKLYERGMQLELVDKDIDPNEYDAEEVKKIIEIALLCTQASAATRPTMSELVVLL 597    
Match NameStatsDescription
A0A2Z6NPU8E-Value: 4.856e-41, PID: 36.78(Uncharacterized protein {ECO:0000313|EMBL:GAU4411... [more]
A0A103Y3T3E-Value: 8.151e-30, PID: 36.60(Gnk2-homologous domain-containing protein {ECO:00... [more]
A0A067LER0E-Value: 2.269e-29, PID: 40.88(Uncharacterized protein {ECO:0000313|EMBL:KDP4696... [more]
A0A1S3VJC3E-Value: 3.173e-29, PID: 39.43(cold-responsive protein kinase 1-like {ECO:000031... [more]
A0A0S3S5N9E-Value: 3.298e-29, PID: 40.25(Uncharacterized protein {ECO:0000313|EMBL:BAT8815... [more]
A0A0L9VCJ6E-Value: 3.531e-29, PID: 40.25(Uncharacterized protein {ECO:0000313|EMBL:KOM5278... [more]
A0A371GYB0E-Value: 1.032e-28, PID: 40.70(Cysteine-rich receptor-like protein kinase 2 {ECO... [more]
A0A2N9GWR1E-Value: 1.325e-28, PID: 46.10(Uncharacterized protein {ECO:0000313|EMBL:SPD0678... [more]
A0A445I578E-Value: 1.712e-28, PID: 45.81(Cysteine-rich receptor-like protein kinase 2 {ECO... [more]
I1LM52E-Value: 1.712e-28, PID: 45.81(Uncharacterized protein {ECO:0000313|EMBL:KRH3080... [more]

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Analysis: 
NameDescription

An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4.

There are a few different naming schemes for this assembly. First there is the
Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below.
These sequences can be viewed in JBrowse here.

Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0

LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926.

Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence.

RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records.

The NCBI Sequence report lists the correspondences between the various naming methods

Link to the LNRQ01000000.1 master record at NCBI

Raw Reads: Link to SRA accessions used for the genome assembly

This genome is available in the CarrotOmics Blast Search

This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions.

For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins.

Data from this analysis can be viewed in JBrowse here.

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