DCAR_008874

Resource Type: 
Polypeptide
Name: 
DCAR_008874
Identifier: 
DCAR_008874.mRNA-protein
Sequence: 
MAPSRVAGGLAGLANSSTNSGIFFQGDRQSHVVGNSHLSSFGNSINSNSG
NLRSNMGPNSGDVSNTLLNSVAASGPSVGASSLVTDANSGLSVGPNLQRS
ASFNTDSYMRGVIDELLYLDLPRECRLSSGVMMLEYGKAVQESVYEQLRV
VREGQLRVIFTPDLKILSWEFCARHHEELLPRRLVAPQVNQLLQVAQKCQ
STITESGSDGVSQQDSQNTSNMMVTAARQFAKSLESQSLNDLGFSKRYVR
CLQISEVVNSMKDLMDFCRDQKDGPMESLKKFPRLASPAKFQLQKMQELE
QMASINGLQTDRGTLNKLVAQNGGLNNQINCNNQMVGRAALSGSPQAAMA
LSNYQNMLMRQNSFNSNSNSAQQEATSSVSNVNQKASPSFQGSASVLSGT
MQNPQTSGFSSSHFLQSHQRALNVNGGLQQNHLQSPQNSQGLQQQMIQRL
LQDINSNNNTGGVQPQQQPLAGNSANVSAGSDGLGYGSNTSMGSAAQVNR
VATTNNPMPTRSNSFKGVSASDSSAAVGNNGNSQRACDIPQNLQLSDELA
QDIAQEFSEHGLFSDLEESMAYGGWKTGL*
Sequence Length: 
580
Sequence Checksum: 
b825ced0688ab7e2ca6751167eedb03d
View location in JBrowse: 
Relationship: 
There is 1 relationship.
Relationships
The polypeptide, DCAR_008874, derives from mRNA, DCAR_008874.
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Blast Results: 
The following BLAST results are available for this feature:
BLAST of DCAR_008874 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
BLAST of DCAR_008874 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B7AG60 ((Putative transcriptional regulator SLK2 {ECO:0000313|EMBL:MPA55699.1}) (1.1.3.15 {ECO:0000313|EMBL:MPA55699.1}))

HSP 1 Score: 595.119 bits (1533), Expect = 0.000e+0
Identity = 324/494 (65.59%), Postives = 380/494 (76.92%), Query Frame = 0
 
Query:   84 VTDANSGLSVGPNLQRSASFNTDSYMRGVIDELLYLDLPRECRLSSGVMMLEYGKAVQESVYEQLRVVREGQLRVIFTPDLKILSWEFCARHHEELLPRRLVAPQVNQLLQVAQKCQSTITESGSDGVSQQDSQNTSNMMVTAARQFAKSLESQSLNDLGFSKRYVRCLQISEVVNSMKDLMDFCRDQKDGPMESLKKFPRLASPAKFQLQKMQELEQMASINGLQTDRGTLNKLVAQNGGLNNQINCNNQMVGRAALSGSPQAAMALSNYQNMLMRQNSFNSNSNSAQQEATSSVSNVNQKASPSFQGSASVLSGTMQNPQTSGFSSSHFLQSHQRALNVNGGLQQNHLQSPQNSQGLQQQMIQRLLQDINSNNNTGGVQPQQQPLAGNSANVSAGSDGLGYGSNTSMGSAAQVNRVATTNNPMPTRSNSFKGVSASDSSAAVGNNGNSQRACDIPQNLQLSDELAQDIAQEFSEHGLF-SDLEESMAYGGWK 576
            +  A SG       +     N   +  GVIDELL+LD+PRECR SSG+MMLEYGKAVQESVYEQLRVVREGQLR+IFTPDLKILSWEFCARHHEELLPRRLVAPQVNQLLQVAQKCQ  I ESGSDGVSQQD Q  SNM++TA RQ  K+LE QSLNDLGFSKRYVRCLQISEVVNSMKDLMDFCR+ K GP+E LK +PR AS AK Q+QKMQE+EQ+A I GL TDR TLNKL+A + GLN+Q+  ++ MVGR ALSGS QAA+AL++YQN+LMRQNS NSNSNS QQEA+SS +N NQ +S +FQGS ++L GT+QN   SGFS+S  L   QR+LN  G LQQNH Q  Q SQ LQQ +IQ+LLQD+N+    GGV  QQQ L G +AN S   DGLG+G+ +S  +AA  N   +   P P+RSNSFK  S SDSSA  GNNG +++A D+ QNL LS+E+ QDIA +F+E+G F SDLE+SM Y GWK
Sbjct:   41 ICGAKSGRGFEATFEVLPRLNEIKFGSGVIDELLFLDMPRECRFSSGIMMLEYGKAVQESVYEQLRVVREGQLRIIFTPDLKILSWEFCARHHEELLPRRLVAPQVNQLLQVAQKCQRAIAESGSDGVSQQDLQTNSNMIMTAQRQLDKTLELQSLNDLGFSKRYVRCLQISEVVNSMKDLMDFCREHKVGPIEGLKNYPRHASAAKLQMQKMQEMEQLAGIQGLPTDRNTLNKLMALHPGLNSQMVNDHHMVGRGALSGSAQAALALTSYQNLLMRQNSMNSNSNSFQQEASSSFNNSNQNSSSAFQGSTALLPGTLQNLAASGFSNSR-LSQQQRSLNGIGLLQQNHAQPSQGSQALQQHVIQQLLQDMNTTTG-GGV--QQQSLGGQNANGSVARDGLGFGNTSSAATAASGNGQGSVMGPTPSRSNSFKAASHSDSSAGGGNNGFNRKAPDLSQNLHLSEEMVQDIAHDFTENGFFNSDLEDSMGY-GWK 529    
BLAST of DCAR_008874 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5J5ATT5 ((Uncharacterized protein {ECO:0000313|EMBL:KAA8533768.1}))

HSP 1 Score: 590.882 bits (1522), Expect = 0.000e+0
Identity = 314/470 (66.81%), Postives = 372/470 (79.15%), Query Frame = 0
 
Query:  111 GVIDELLYLDLPRECRLSSGVMMLEYGKAVQESVYEQLRVVREGQLRVIFTPDLKILSWEFCARHHEELLPRRLVAPQVNQLLQVAQKCQSTITESGSDGVSQQDSQNTSNMMVTAARQFAKSLESQSLNDLGFSKRYVRCLQISEVVNSMKDLMDFCRDQKDGPMESLKKFPRLASPAKFQLQKMQELEQMASINGLQTDRGTLNKLVAQNGGLNNQINCNNQMVGRAALSGSPQAAMALSNYQNMLMRQNSFNSNSNSAQQEATSSVSNVNQKASPSFQGSASVLSGTMQNPQTSGFSSS---HFLQSHQRALNVNGGLQQNHLQSPQNSQGLQQQMIQRLLQDINSNNNTGGVQPQQQPLAGNSANVSAGSDGLGYGSNTSMGSAAQVNRVATTNNPMPTRSNSFKGVSASDSSAAVGNNGNSQRACDIPQNLQLSDELAQDIAQEFSEHGLF-SDLEESMAYGGWK 576
            GVIDELL+LD+PRECR  SG+MMLEYGKAVQESVYEQLRVVREGQLR+IFTPDLKILSWEFCAR HEELLPRRLVAPQVNQ LQVAQKCQSTI ESGSDGVSQQD Q  SNM+VTA RQ A++LE QSLNDLGFSKRYVRCLQISEVVNSMKDLMDFC +QK GP+E LK +P+ A+ AK Q+QKMQE+EQ+A I GL TDR TL+KL+    GLN+Q+  N+ +VGR  LSGS QAA+A++NYQ++LMRQNS NSNSN   QEA+SS +N NQ  S +FQG A +L G +QN  +SGFS+S      Q  QR+LNVNG LQQNH Q  Q SQ LQQ MIQ+LLQD+  NN+ GGV  QQQ LAG + N S   D +G+GSNTS  +AA +N       P P+RSNSFK VS SDSSAA GN+G +Q+A D+PQ+L L +++ QDIA++F+E+G F SDL+++M Y GWK
Sbjct:   35 GVIDELLFLDMPRECRSPSGIMMLEYGKAVQESVYEQLRVVREGQLRIIFTPDLKILSWEFCARRHEELLPRRLVAPQVNQFLQVAQKCQSTIAESGSDGVSQQDLQTNSNMVVTAWRQLARTLELQSLNDLGFSKRYVRCLQISEVVNSMKDLMDFCGEQKVGPIEGLKNYPQHATAAKLQMQKMQEMEQLAGIQGLPTDRNTLSKLMTVYPGLNSQMGNNHHIVGRGTLSGSAQAALAMTNYQSLLMRQNSMNSNSNPLHQEASSSFNNSNQNPSSTFQGPAGLLQGMLQNLPSSGFSNSQFPQQQQLQQRSLNVNGLLQQNHPQPSQGSQTLQQHMIQQLLQDM-KNNSGGGV--QQQSLAGQNVNGSVRRDTIGFGSNTSTATAAPINGPGNIMGPTPSRSNSFKAVSNSDSSAAGGNSGFNQKAPDLPQDLHLPEDMVQDIARDFTENGFFNSDLDDNMGY-GWK 500    
BLAST of DCAR_008874 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B7AJM8 ((Putative transcriptional regulator SLK2 {ECO:0000313|EMBL:MPA55701.1}) (1.1.3.15 {ECO:0000313|EMBL:MPA55701.1}))

HSP 1 Score: 587.8 bits (1514), Expect = 0.000e+0
Identity = 321/479 (67.01%), Postives = 375/479 (78.29%), Query Frame = 0
 
Query:  103 FNTDSYMRGVIDELLYLDLPRECRLSSGVMMLEYGKAVQESVYEQLRVVREGQLRVIFTPDLKILSWEFCARHHEELLPRRLVAPQVNQLLQVAQKCQSTITESGSDGVSQQDSQNTSNMMVTAARQFAKSLESQSLNDLGFSKRYVRCLQISEVVNSMKDLMDFCRDQKDGPMESLKKFPRLASPAKFQLQKMQELEQMASINGLQTDRGTLNKLVAQNGGLNNQINCNNQMVGRAALSGSPQAAMALSNYQNMLMRQNSFNSNSNSAQQEATSSVSNVNQKASPSFQGSASVLSGTMQNPQTSGFSSSHF----LQSHQRALNVNGGLQQNHLQSPQNSQGLQQQMIQRLLQDINSNNNTGGVQPQQQPLAGNSANVSAGSDGLGYGSNTSMGSAAQVNRVATTNNPMPTRSNSFKGVSASDSSAAVGNNGNSQRACDIPQNLQLSDELAQDIAQEFSEHGLF-SDLEESMAYGGWK 576
             N   +  GVIDELL+LD+PRECR SSG+MMLEYGKAVQESVYEQLRVVREGQLR+IFTPDLKILSWEFCARHHEELLPRRLVAPQVNQLLQVAQKCQ  I ESGSDGVSQQD Q  SNM++TA RQ  K+LE QSLNDLGFSKRYVRCLQISEVVNSMKDLMDFCR+ K GP+E LK +PR AS AK Q+QKMQE+EQ+A I GL TDR TLNKL+A + GLN+Q+  ++ MVGR ALSGS QAA+AL++YQN+LMRQNS NSNSNS QQEA+SS +N NQ +S +FQGS ++L GT+QN   SGFS+S       Q  QR+LN  G LQQNH Q  Q SQ LQQ +IQ+LLQD+N+    GGV  QQQ L G +AN S   DGLG+G+ +S  +AA  N   +   P P+RSNSFK  S SDSSA  GNNG +++A D+ QNL LS+E+ QDIA +F+E+G F SDLE+SM Y GWK
Sbjct:  383 LNEIKFGSGVIDELLFLDMPRECRFSSGIMMLEYGKAVQESVYEQLRVVREGQLRIIFTPDLKILSWEFCARHHEELLPRRLVAPQVNQLLQVAQKCQRAIAESGSDGVSQQDLQTNSNMIMTAQRQLDKTLELQSLNDLGFSKRYVRCLQISEVVNSMKDLMDFCREHKVGPIEGLKNYPRHASAAKLQMQKMQEMEQLAGIQGLPTDRNTLNKLMALHPGLNSQMVNDHHMVGRGALSGSAQAALALTSYQNLLMRQNSMNSNSNSFQQEASSSFNNSNQNSSSAFQGSTALLPGTLQNLAASGFSNSRLSQQQQQQQQRSLNGIGLLQQNHAQPSQGSQALQQHVIQQLLQDMNTTTG-GGV--QQQSLGGQNANGSVARDGLGFGNTSSAATAASGNGQGSVMGPTPSRSNSFKAASHSDSSAGGGNNGFNRKAPDLSQNLHLSEEMVQDIAHDFTENGFFNSDLEDSMGY-GWK 857    

HSP 2 Score: 135.961 bits (341), Expect = 7.762e-31
Identity = 78/111 (70.27%), Postives = 91/111 (81.98%), Query Frame = 0
 
Query:    1 MAPSRVAGGLAGLANSSTNSGIFFQGDRQSHVVGNSHLSSFGNSINSN-SGNLRSNMGPNSGDVSNTLLNSVAASGPSVGASSLVTDANSGLSVGPNLQRSASFNTDSYMR 110
            M PSRVAGGL    +SS++SGI FQGD QS  VGNSHLSS   + +++  G  RSN+GP SGDVSNT+LNSVA+SGPSVGASSLVTDANSGLS GP+LQRSAS NT+SY+R
Sbjct:    1 MMPSRVAGGLT---HSSSSSGICFQGDGQSQAVGNSHLSSSFGNSSNSIPGTARSNLGPISGDVSNTILNSVASSGPSVGASSLVTDANSGLSGGPHLQRSASINTESYLR 108    
BLAST of DCAR_008874 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5B7AG29 ((Putative SEUSS-like 2 {ECO:0000313|EMBL:MPA55700.1}) (1.1.3.15 {ECO:0000313|EMBL:MPA55700.1}))

HSP 1 Score: 587.03 bits (1512), Expect = 0.000e+0
Identity = 320/497 (64.39%), Postives = 377/497 (75.86%), Query Frame = 0
 
Query:   84 VTDANSGLSVGPNLQRSASFNTDSYMRGVIDELLYLDLPRECRLSSGVMMLEYGKAVQESVYEQLRVVREGQLRVIFTPDLKILSWEFCARHHEELLPRRLVAPQVNQLLQVAQKCQSTITESGSDGVSQQDSQNTSNMMVTAARQFAKSLESQSLNDLGFSKRYVRCLQISEVVNSMKDLMDFCRDQKDGPMESLKKFPRLASPAKFQLQKMQELEQMASINGLQTDRGTLNKLVAQNGGLNNQINCNNQMVGRAALSGSPQAAMALSNYQNMLMRQNSFNSNSNSAQQEATSSVSNVNQKASPSFQGSASVLSGTMQNPQTSGFSSS---HFLQSHQRALNVNGGLQQNHLQSPQNSQGLQQQMIQRLLQDINSNNNTGGVQPQQQPLAGNSANVSAGSDGLGYGSNTSMGSAAQVNRVATTNNPMPTRSNSFKGVSASDSSAAVGNNGNSQRACDIPQNLQLSDELAQDIAQEFSEHGLF-SDLEESMAYGGWK 576
            +  A SG       +     N   +  GVIDELL+LD+PRECR SSG+MMLEYGKAVQESVYEQLRVVREGQLR+IFTPDLKILSWEFCARHHEELLPRRLVAPQVNQLLQVAQKCQ  I ESGSDGVSQQD Q  SNM+V A RQ +++LE QSLNDLGFSKRYVRCLQISEVVNSMKDLMDFCR+ K GP+E LK +P+ A+ AK Q+Q+M++L       GL TDR TLNKL+AQ+ G+N+Q+  ++QMVGR ALSGS QAA+ALS+YQN+LMRQNS NSNSNS  QEA+ S +N NQ  S +FQG  ++L GT+QN  +SGFS+S      Q  QR+LN NG LQQNH Q  Q SQ LQQ MIQ+LLQD+  NN  GGV  QQQ  AG +AN S G DG+G+GSN S  +AA VN   +   P P+RSNSFK  S  DSSAA GNNG +Q+A D+ QNLQLSDE+ QDIA + +E+G F SDLE++M+Y  WK
Sbjct:  364 ICGAKSGRGFEATFEVLPRLNEIKFGSGVIDELLFLDMPRECRFSSGIMMLEYGKAVQESVYEQLRVVREGQLRIIFTPDLKILSWEFCARHHEELLPRRLVAPQVNQLLQVAQKCQRAIAESGSDGVSQQDLQTNSNMVVAAWRQLSRTLELQSLNDLGFSKRYVRCLQISEVVNSMKDLMDFCREHKAGPIEGLKNYPQHATAAKLQMQEMEQL-------GLPTDRNTLNKLMAQHPGMNSQMGNSHQMVGRGALSGSAQAALALSSYQNLLMRQNSMNSNSNSLHQEASPSFNNSNQNPSSTFQGPTALLPGTLQNLPSSGFSNSQLPQQQQMQQRSLNGNGLLQQNHPQPSQGSQALQQHMIQQLLQDMK-NNTGGGV--QQQSHAGQNANGSVGRDGMGFGSNPSAATAASVNGPGSIMGPTPSRSNSFKAASNIDSSAAGGNNGFNQKAPDLSQNLQLSDEMVQDIAHDLTENGFFNSDLEDNMSY-CWK 849    

HSP 2 Score: 134.42 bits (337), Expect = 2.579e-30
Identity = 77/111 (69.37%), Postives = 91/111 (81.98%), Query Frame = 0
 
Query:    1 MAPSRVAGGLAGLANSSTNSGIFFQGDRQSHVVGNSHLSSFGNSINSN-SGNLRSNMGPNSGDVSNTLLNSVAASGPSVGASSLVTDANSGLSVGPNLQRSASFNTDSYMR 110
            M PSRVAGGL    +SS++SG+ FQGD QS  VGNSHLSS   + +++  G  RSN+GP SGDVSNT+LNSVA+SGPSVGASSLVTDANSGLS GP+LQRSAS NT+SY+R
Sbjct:    1 MMPSRVAGGLT---HSSSSSGLCFQGDGQSQAVGNSHLSSSFGNSSNSIPGTARSNLGPISGDVSNTILNSVASSGPSVGASSLVTDANSGLSGGPHLQRSASINTESYLR 108    
BLAST of DCAR_008874 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5J5BBV1 ((Uncharacterized protein {ECO:0000313|EMBL:KAA8539880.1}))

HSP 1 Score: 586.645 bits (1511), Expect = 0.000e+0
Identity = 317/475 (66.74%), Postives = 369/475 (77.68%), Query Frame = 0
 
Query:  103 FNTDSYMRGVIDELLYLDLPRECRLSSGVMMLEYGKAVQESVYEQLRVVREGQLRVIFTPDLKILSWEFCARHHEELLPRRLVAPQVNQLLQVAQKCQSTITESGSDGVSQQDSQNTSNMMVTAARQFAKSLESQSLNDLGFSKRYVRCLQISEVVNSMKDLMDFCRDQKDGPMESLKKFPRLASPAKFQLQKMQELEQMASINGLQTDRGTLNKLVAQNGGLNNQINCNNQMVGRAALSGSPQAAMALSNYQNMLMRQNSFNSNSNSAQQEATSSVSNVNQKASPSFQGSASVLSGTMQNPQTSGFSSSHFLQSHQRALNVNGGLQQNHLQSPQNSQGLQQQMIQRLLQDINSNNNTGGVQPQQQPLAGNSANVSAGSDGLGYGSNTSMGSAAQVNRVATTNNPMPTRSNSFKGVSASDSSAAVGNNGNSQRACDIPQNLQLSDELAQDIAQEFSEHGLF-SDLEESMAYGGWK 576
             N   +  GVIDELL+LDLPRECR  SG+MMLEYGKAVQESVYEQLRVVREGQLRVIFTPDLKILSWEFCAR HEELLPRRLVAPQVNQLLQVAQKCQ  I ESGSDGVSQQD Q  SNM+V A RQ  ++LE QSLNDLGFSKRYVRCLQISEVVNSMKD+MDFCR+ K GP+E LK +PR  + AK Q+QKMQE+EQ+A I GL TDR TLN+L+  + GLN+Q+  ++ M GR ALSG  QAA+AL++YQNMLMRQNS NSNSNS QQEA+SS +N NQ +SP+FQGS + L GT+QN   SGFS+S   Q  Q +LN  G  QQNH Q  Q SQ LQQ MIQ+LLQD+  NN TGG  PQQ  L+G +AN S   DGLGYG+N S  +AA  N   +   P P+R+NSFK  S SDSSAA GNNG +Q+  D+PQNL LS+E+ QDIA +F+E+G F SDLE++M+Y GWK
Sbjct:  381 LNEIKFGSGVIDELLFLDLPRECRFPSGIMMLEYGKAVQESVYEQLRVVREGQLRVIFTPDLKILSWEFCARRHEELLPRRLVAPQVNQLLQVAQKCQRAIAESGSDGVSQQDLQTNSNMIVAAQRQLDRTLELQSLNDLGFSKRYVRCLQISEVVNSMKDVMDFCREHKIGPIEGLKNYPRHGTAAKLQMQKMQEMEQLAGIQGLPTDRNTLNQLMTLHPGLNSQMGNDHHMAGRGALSGPAQAALALNSYQNMLMRQNSMNSNSNSLQQEASSSFNNPNQNSSPTFQGSTAQLPGTLQNLAASGFSNSRLPQQQQHSLNGIGLQQQNHAQPSQGSQALQQHMIQQLLQDM--NNKTGGGVPQQS-LSGQNANGSVARDGLGYGNNNSAATAASGNGQGSVMGPTPSRNNSFKAASHSDSSAAGGNNGFNQKESDVPQNLHLSEEMVQDIAHDFTENGFFNSDLEDNMSY-GWK 851    

HSP 2 Score: 127.487 bits (319), Expect = 4.673e-28
Identity = 74/111 (66.67%), Postives = 89/111 (80.18%), Query Frame = 0
 
Query:    1 MAPSRVAGGLAGLANSSTNSGIFFQGDRQSHVVGNSHLSSFGNSINSN-SGNLRSNMGPNSGDVSNTLLNSVAASGPSVGASSLVTDANSGLSVGPNLQRSASFNTDSYMR 110
            M PSR AGGL    +SS++SG+FFQGD QS  +GNS LSS   + +++  G  RSN+GP SGDVSN++LNSV +SGPSVGASSLVTDANSGLS GP+LQRSAS NT+SYMR
Sbjct:    1 MVPSRGAGGLT---HSSSSSGLFFQGDVQSQALGNSRLSSSFGNSSNSIPGTGRSNLGPVSGDVSNSVLNSVVSSGPSVGASSLVTDANSGLSGGPHLQRSASINTESYMR 108    
BLAST of DCAR_008874 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2K1YD61 ((Uncharacterized protein {ECO:0000313|EMBL:PNT10970.1}))

HSP 1 Score: 573.933 bits (1478), Expect = 0.000e+0
Identity = 311/481 (64.66%), Postives = 370/481 (76.92%), Query Frame = 0
 
Query:  103 FNTDSYMRGVIDELLYLDLPRECRLSSGVMMLEYGKAVQESVYEQLRVVREGQLRVIFTPDLKILSWEFCARHHEELLPRRLVAPQVNQLLQVAQKCQSTITESGSDGVSQQDSQNTSNMMVTAARQFAKSLESQSLNDLGFSKRYVRCLQISEVVNSMKDLMDFCRDQKDGPMESLKKFPRLASPAKFQLQKMQELEQMASINGLQTDRGTLNKLVAQNGGLNNQINCNNQMVGRAALSGSPQAAMALSNYQNMLMRQNSFNSNSNSAQQEATSSVSNVNQKASPSFQGSASVLSGTMQNPQTSGFSS-----SHFLQSHQRALNVNGGLQQNHLQSPQNSQGLQQQMIQRLLQDINSNNNTGGVQPQQQPLAGNSANVSAGSDGLGYGSNTSMGSAAQVNRVATT-NNPMPTRSNSFKGVSASDSSAAVGNNGNSQRACDIPQNLQLSDELAQDIAQEFSEHGLF-SDLEESMAYGGWK 576
             N   +  GVIDELL+LDLPRE RL SG+MMLEY KAVQESVYEQLRVVREGQLR+IFTPDLKILSWEFCAR HEELLPRR+VAPQVNQLLQVAQKCQSTI ESGSDGVSQQD Q  SNM++TA RQ AKSLE QSLNDLGFSKRYVRCLQISEVVNSMKDL+DFCR+QK GP+E LK +PR A+ AK Q+QKMQE+EQ+AS+ GL TDR T+NKL+A + G+NN +N NNQMVGR ALSGS QAA+AL+NYQN+LMRQNS NSNS S QQEA S  SN NQ  S +FQG+A+ + G+MQN   SGFSS         Q  QR+L+ N  LQQ+  +S   +Q LQ QMI +LLQ++ SNN+ GGV  QQ  ++  S N      GLG+GSN SM +A   + V+ +   P P++SNSFK  + SDSSAA GN+G +Q+  D+PQNL L D++  DIA EF+E+G F SDL+++M Y GWK
Sbjct:  263 LNEIKFGSGVIDELLFLDLPREFRLHSGIMMLEYAKAVQESVYEQLRVVREGQLRIIFTPDLKILSWEFCARRHEELLPRRVVAPQVNQLLQVAQKCQSTIAESGSDGVSQQDLQTNSNMVLTAGRQLAKSLELQSLNDLGFSKRYVRCLQISEVVNSMKDLIDFCREQKAGPIEGLKSYPRHATAAKLQMQKMQEMEQLASVQGLPTDRNTINKLMALHPGINNHVNSNNQMVGRGALSGSAQAALALTNYQNLLMRQNSMNSNSCSLQQEAASPFSNSNQSPSSNFQGAANFIQGSMQNLPVSGFSSPHPPPQQPQQLQQRSLSSNSLLQQSLPRSSHGNQTLQPQMIHQLLQEM-SNNSGGGV--QQHSISRQSGNGGVARMGLGFGSN-SMATAPTASTVSVSAGGPAPSQSNSFKAPANSDSSAAGGNSGFNQKVPDLPQNLHLQDDIVSDIAHEFTENGFFNSDLDDNMGY-GWK 738    
BLAST of DCAR_008874 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5N5KPY6 ((Uncharacterized protein {ECO:0000313|EMBL:KAB5532423.1}))

HSP 1 Score: 570.852 bits (1470), Expect = 0.000e+0
Identity = 312/491 (63.54%), Postives = 368/491 (74.95%), Query Frame = 0
 
Query:  103 FNTDSYMRGVIDELLYLDLPRECRLSSGVMMLEYGKAVQESVYEQLRVVREGQLRVIFTPDLKILSWEFCARHHEELLPRRLVAPQVNQLLQVAQKCQSTITESGSDGVSQQDSQNTSNMMVTAARQFAKSLESQSLNDLGFSKRYVRCLQISEVVNSMKDLMDFCRDQKDGPMESLKKFPRLASPAKFQLQKMQELEQMASING----------LQTDRGTLNKLVAQNGGLNNQINCNNQMVGRAALSGSPQAAMALSNYQNMLMRQNSFNSNSNSAQQEATSSVSNVNQKASPSFQGSASVLSGTMQNPQTSGFSSSH----FLQSHQRALNVNGGLQQNHLQSPQNSQGLQQQMIQRLLQDINSNNNTGGVQPQQQPLAGNSANVSAGSDGLGYGSNTSMGSAAQVNRVA-TTNNPMPTRSNSFKGVSASDSSAAVGNNGNSQRACDIPQNLQLSDELAQDIAQEFSEHGLF-SDLEESMAYGGWKT 577
             N   +  GVIDELL+LDLPRE RL SG+MMLEY KAVQESVYEQLRVVREGQLR+IFT DLKILSWEFCAR HEELLPRR+VAPQVNQLLQVAQKCQSTI+ESGSDGVSQQD Q  SNM++TA RQ AKSLE QSLNDLGFSKRYVRCLQISEVVNSMKDL+DFCR+QK GP+E LK +PR A+ AK QLQKMQE+EQ AS+ G          L TDR TLNKL+A + G NN +N NNQM GR ALSGS QAA+AL+NYQN+LMRQNS NSNS S QQEA S  SN NQ  S +FQG+A+ + G+MQN   SGFSS H      Q  QR+L+ N  LQQN  +S   +Q LQ QMIQ+LLQ+++SN+  G    QQ  ++G S N     +GLG+GSN  + +A   + V+ +T  P P RSNSFK  + SDSSAAVGN+G +Q+  D+PQNL L D +  D+A EF+E+G F SDL+++M Y GWK 
Sbjct:  380 LNEIKFGSGVIDELLFLDLPREFRLPSGIMMLEYAKAVQESVYEQLRVVREGQLRIIFTQDLKILSWEFCARRHEELLPRRVVAPQVNQLLQVAQKCQSTISESGSDGVSQQDLQANSNMVLTAGRQLAKSLELQSLNDLGFSKRYVRCLQISEVVNSMKDLIDFCREQKAGPIEGLKSYPRHATAAKLQLQKMQEMEQFASVQGLPTDRNGVQSLPTDRNTLNKLMALHPGANNHVNSNNQMAGRGALSGSAQAALALTNYQNLLMRQNSMNSNSCSLQQEAASPFSNSNQSPSSNFQGAANFIPGSMQNLSASGFSSPHLPQQPQQLQQRSLSSNSLLQQNLSRSSHGNQTLQPQMIQQLLQEMSSNSGAG---VQQHSISGQSGNDGVARNGLGFGSNC-LATAPTASAVSVSTGGPAPNRSNSFKAPANSDSSAAVGNSGFNQKVPDLPQNLHLQDVIVSDMAHEFTENGFFNSDLDDNMGY-GWKA 865    

HSP 2 Score: 122.479 bits (306), Expect = 2.040e-26
Identity = 73/111 (65.77%), Postives = 86/111 (77.48%), Query Frame = 0
 
Query:    1 MAPSRVAGGLAGLANSSTNSGIFFQGDRQSHVVGNSHLSSFGNSINSN-SGNLRSNMGPNSGDVSNTLLNSVAASGPSVGASSLVTDANSGLSVGPNLQRSASFNTDSYMR 110
            MAPSRVAG LA    SS++SGIFFQGD QS  + NSHLSS   + +++  G  R N+GP SGD++N + NSVA SGPSVGASSLVTDANS LS GP+LQRSAS NT+SYMR
Sbjct:    1 MAPSRVAGALA---QSSSSSGIFFQGDGQSQGLVNSHLSSSFGNSSNSIPGTGRPNLGPVSGDMNNAVFNSVANSGPSVGASSLVTDANSALSGGPHLQRSASINTESYMR 108    
BLAST of DCAR_008874 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A4V6A988 ((Uncharacterized protein {ECO:0000313|EMBL:TKS04486.1}))

HSP 1 Score: 570.852 bits (1470), Expect = 0.000e+0
Identity = 310/482 (64.32%), Postives = 369/482 (76.56%), Query Frame = 0
 
Query:  103 FNTDSYMRGVIDELLYLDLPRECRLSSGVMMLEYGKAVQESVYEQLRVVREGQLRVIFTPDLKILSWEFCARHHEELLPRRLVAPQVNQLLQVAQKCQSTITESGSDGVSQQDSQNTSNMMVTAARQFAKSLESQSLNDLGFSKRYVRCLQISEVVNSMKDLMDFCRDQKDGPMESLKKFPRLASPAKFQLQKMQELEQMASINGLQTDRGTLNKLVAQNGGLNNQINCNNQMVGRAALSGSPQAAMALSNYQNMLMRQNSFNSNSNSAQQEATSSVSNVNQKASPSFQGSASVLSGTMQNPQTSGFSS-----SHFLQSHQRALNVNGGLQQNHLQSPQNSQGLQQQMIQRLLQDINSNNNTGGVQPQQQPLAGNSANVSAGSDGLGYGSNTSMGSAAQVNRVATT-NNPMPTRSNSFKGVSASDSSAAVGNNGNSQRACDIPQNLQLSDELAQDIAQEFSEHGLF-SDLEESMAYGGWKT 577
             N   +  GVIDELL+LDLPRE RL SG+MMLEY KAVQESVYEQLRVVREGQLR+IFTP+LKILSWEFCAR HEELLPRR+VAPQVNQLLQVAQKCQSTI ESGSDGVSQQD Q  SNM++TA RQ AKSLE QSLNDLGFSKRYVRCLQISEVVNSMKDL+DFCR+QK GP+E LK +PR A+ AK Q+QKMQE+EQ+AS  GL TDR TLNKL+A + G+NN +N NNQMVGR ALSGS QAA+AL+NYQN+LMRQNS NSNS S QQEA S  SN NQ  S +FQG A+ + G+MQN   SGFSS         Q  QR+L+ N  LQQ+  +S   +Q LQ QMI +LLQ++ SNN+ GGV  QQ  ++  S N     +GLG+GSN S+ +A   + V+ +   P P++SNSFK  + SDSSAA GN+G +Q+  D+PQNL L D++  DIA EF+E+G F SDL+++M Y GWK 
Sbjct:  381 LNEIKFGSGVIDELLFLDLPREFRLPSGLMMLEYAKAVQESVYEQLRVVREGQLRIIFTPELKILSWEFCARRHEELLPRRVVAPQVNQLLQVAQKCQSTIAESGSDGVSQQDLQTNSNMVLTAGRQLAKSLELQSLNDLGFSKRYVRCLQISEVVNSMKDLIDFCREQKAGPIEGLKSYPRHATAAKLQMQKMQEMEQLASAQGLPTDRNTLNKLMALHPGINNHVNSNNQMVGRGALSGSAQAALALTNYQNLLMRQNSMNSNSCSLQQEAASPFSNSNQSPSSNFQGGANFIPGSMQNLPVSGFSSPHPPPQQPQQLQQRSLSSNSLLQQSLPRSSHGNQTLQPQMIHQLLQEM-SNNSGGGV--QQHSISRQSGNGGLARNGLGFGSN-SLATAPTASTVSVSAGGPAPSQSNSFKAPANSDSSAAGGNSGFNQKVPDLPQNLHLQDDIVSDIAHEFTENGFFNSDLDDNMGY-GWKA 857    

HSP 2 Score: 119.013 bits (297), Expect = 2.680e-25
Identity = 72/111 (64.86%), Postives = 85/111 (76.58%), Query Frame = 0
 
Query:    1 MAPSRVAGGLAGLANSSTNSGIFFQGDRQSHVVGNSHLSSFGNSINSN-SGNLRSNMGPNSGDVSNTLLNSVAASGPSVGASSLVTDANSGLSVGPNLQRSASFNTDSYMR 110
            M PSRVAG LA    SS++SGIFFQGD QS  + NS LSS   + +++  G  R N+GP SGD++N +LNSVA SGPSVGASSLVTDANS LS GP+LQRSAS NT+SYMR
Sbjct:    1 MVPSRVAGALA---QSSSSSGIFFQGDGQSQGLVNSRLSSSFGNSSNSIPGTGRPNLGPVSGDMNNAVLNSVANSGPSVGASSLVTDANSALSGGPHLQRSASINTESYMR 108    
BLAST of DCAR_008874 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A837AV31 ((Transcriptional regulator SLK {ECO:0000313|EMBL:KAG5234534.1}))

HSP 1 Score: 570.466 bits (1469), Expect = 0.000e+0
Identity = 314/491 (63.95%), Postives = 372/491 (75.76%), Query Frame = 0
 
Query:  103 FNTDSYMRGVIDELLYLDLPRECRLSSGVMMLEYGKAVQESVYEQLRVVREGQLRVIFTPDLKILSWEFCARHHEELLPRRLVAPQVNQLLQVAQKCQSTITESGSDGVSQQDSQNTSNMMVTAARQFAKSLESQSLNDLGFSKRYVRCLQISEVVNSMKDLMDFCRDQKDGPMESLKKFPRLASPAKFQLQKMQELEQMASING----------LQTDRGTLNKLVAQNGGLNNQINCNNQMVGRAALSGSPQAAMALSNYQNMLMRQNSFNSNSNSAQQEATSSVSNVNQKASPSFQGSASVLSGTMQNPQTSGFSSSH----FLQSHQRALNVNGGLQQNHLQSPQNSQGLQQQMIQRLLQDINSNNNTGGVQPQQQPLAGNSANVSAGSDGLGYGSNTSMGSAAQVNRVA-TTNNPMPTRSNSFKGVSASDSSAAVGNNGNSQRACDIPQNLQLSDELAQDIAQEFSEHGLF-SDLEESMAYGGWKT 577
             N   +  GVIDELL+LDLPRE RL SG+MMLEY KAVQESVYEQLRVVREGQLR+IFT DLKILSWEFCAR HEELLPRR+VAPQVNQLLQVAQKCQSTI+ESGSDGVSQQD Q  SNM++TA RQ AKSLE QSLNDLGFSKRYVRCLQISEVVNSMKDL+DFCR+QK GP+E LK +PR A+ AK QLQKMQE+EQ AS+ G          L TDR TLNKL+A + G NN +N NNQM GR ALSGS QAA+AL+NYQN+LMRQNS NSNS S QQEA S  SN NQ  S +FQG+A+ + G+MQN   SGFSS H      Q  QR+L+ N  LQQN  +S   +Q LQ QMIQ+LLQ+++SN+  GGV  QQ  ++G S N     +GLG+GSN  + +A+  + V+ +T  P P+RSNSFK  + SDSSAAVGN+G +Q+  D+ QNL L D +A D+A EF+E+G F SDL+++M Y GWK 
Sbjct:  383 LNEIKFGSGVIDELLFLDLPRELRLPSGIMMLEYAKAVQESVYEQLRVVREGQLRIIFTQDLKILSWEFCARRHEELLPRRVVAPQVNQLLQVAQKCQSTISESGSDGVSQQDLQANSNMVLTAGRQLAKSLELQSLNDLGFSKRYVRCLQISEVVNSMKDLIDFCREQKAGPIEGLKSYPRHATAAKLQLQKMQEMEQFASVQGLPTDRNGVQSLPTDRNTLNKLMALHPGANNHVNSNNQMAGRGALSGSAQAALALTNYQNLLMRQNSMNSNSCSLQQEAASPFSNSNQSPSSNFQGAANFIPGSMQNLSASGFSSPHLPQQPQQLQQRSLSSNSLLQQNLSRSSHGNQTLQPQMIQQLLQEMSSNSG-GGV--QQHSISGQSGNDGVARNGLGFGSNR-LATASTASAVSVSTGGPAPSRSNSFKAPANSDSSAAVGNSGFNQKLQDMSQNLHLQDVIASDMAHEFTENGFFNSDLDDNMGY-GWKA 868    

HSP 2 Score: 122.479 bits (306), Expect = 1.758e-26
Identity = 73/111 (65.77%), Postives = 86/111 (77.48%), Query Frame = 0
 
Query:    1 MAPSRVAGGLAGLANSSTNSGIFFQGDRQSHVVGNSHLSSFGNSINSN-SGNLRSNMGPNSGDVSNTLLNSVAASGPSVGASSLVTDANSGLSVGPNLQRSASFNTDSYMR 110
            MAPSRVAG LA    SS++SGIFFQGD QS  + NSHLSS   + +++  G  R N+GP SGD++N + NSVA SGPSVGASSLVTDANS LS GP+LQRSAS NT+SYMR
Sbjct:    1 MAPSRVAGALA---QSSSSSGIFFQGDGQSQGLVNSHLSSSFGNSSNSIPGTGRPNLGPVSGDMNNAVFNSVANSGPSVGASSLVTDANSALSGGPHLQRSASINTESYMR 108    
BLAST of DCAR_008874 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A835JP41 ((Uncharacterized protein {ECO:0000313|EMBL:KAF9671979.1}))

HSP 1 Score: 566.229 bits (1458), Expect = 0.000e+0
Identity = 314/491 (63.95%), Postives = 368/491 (74.95%), Query Frame = 0
 
Query:  103 FNTDSYMRGVIDELLYLDLPRECRLSSGVMMLEYGKAVQESVYEQLRVVREGQLRVIFTPDLKILSWEFCARHHEELLPRRLVAPQVNQLLQVAQKCQSTITESGSDGVSQQDSQNTSNMMVTAARQFAKSLESQSLNDLGFSKRYVRCLQISEVVNSMKDLMDFCRDQKDGPMESLKKFPRLASPAKFQLQKMQELEQMASING----------LQTDRGTLNKLVAQNGGLNNQINCNNQMVGRAALSGSPQAAMALSNYQNMLMRQNSFNSNSNSAQQEATSSVSNVNQKASPSFQGSASVLSGTMQNPQTSGFSSSH----FLQSHQRALNVNGGLQQNHLQSPQNSQGLQQQMIQRLLQDINSNNNTGGVQPQQQPLAGNSANVSAGSDGLGYGSNTSMGSAAQVNRVA-TTNNPMPTRSNSFKGVSASDSSAAVGNNGNSQRACDIPQNLQLSDELAQDIAQEFSEHGLF-SDLEESMAYGGWKT 577
             N   +  GVIDELL+LDLPRE RL SG+MMLEY KAVQESVYEQLRVVREGQLR+IFT DLKILSWEFCAR HEELLPRR+VAPQVNQLL VAQKCQSTI+ESGSDGVSQQD Q  SNM++TA RQ AKSLE QSLNDLGFSKRYVRCLQISEVVNSMKDL+DFCR+QK GP+E LK +PR A+ AK QLQKMQE+EQ AS+ G          L TDR TLNKL+A + G NN +N NNQM GR ALSGS QAA+AL+NYQN+LMRQNS NSNS S QQEA S  SN NQ  S +FQG+A+ + G+MQN   SGFSS H      Q  QR+L+ N  LQQN  +S   +Q LQ QMIQ+LLQ+++SN+  GGV  QQ  ++G S N     +GLG+GSN  + +A   + V+ +T  P P+RSNSFK  + SDSSAAV NNG +Q+  D+ QNL L D +  DIA EF+E+G F SDLE++M Y GWK 
Sbjct:  409 LNEIKFGSGVIDELLFLDLPRESRLPSGIMMLEYAKAVQESVYEQLRVVREGQLRIIFTQDLKILSWEFCARRHEELLPRRVVAPQVNQLLHVAQKCQSTISESGSDGVSQQDLQANSNMVLTAGRQLAKSLELQSLNDLGFSKRYVRCLQISEVVNSMKDLIDFCREQKAGPIEGLKSYPRHAT-AKLQLQKMQEMEQFASVQGLPTDRNGAQSLTTDRNTLNKLMALHPGANNHVNSNNQMAGRGALSGSAQAALALTNYQNLLMRQNSMNSNSCSLQQEAASPFSNSNQSPSSNFQGAANFIPGSMQNLSVSGFSSPHLPQQPQQLQQRSLSSNSLLQQNLPRSSHGNQTLQPQMIQQLLQEMSSNSG-GGV--QQHSISGQSGNDGVARNGLGFGSN-HLATAPTASTVSISTGGPAPSRSNSFKAPANSDSSAAVNNNGFNQKVPDLHQNLHLQDVIVNDIAHEFTENGFFNSDLEDNMGY-GWKA 893    

HSP 2 Score: 123.635 bits (309), Expect = 8.066e-27
Identity = 74/111 (66.67%), Postives = 87/111 (78.38%), Query Frame = 0
 
Query:    1 MAPSRVAGGLAGLANSSTNSGIFFQGDRQSHVVGNSHLSSFGNSINSN-SGNLRSNMGPNSGDVSNTLLNSVAASGPSVGASSLVTDANSGLSVGPNLQRSASFNTDSYMR 110
            MAPSRVAG LA    SS++SGIFFQGD QS  + NSHLSS   + +++  G  R N+GP SGD++N +LNSVA SGPSVGASSLVTDANS LS GP+LQRSAS NT+SYMR
Sbjct:   18 MAPSRVAGALA---QSSSSSGIFFQGDGQSQGLVNSHLSSSFGNSSNSIPGTGRPNLGPVSGDMNNAVLNSVANSGPSVGASSLVTDANSALSGGPHLQRSASINTESYMR 125    
Match NameStatsDescription
A0A5B7AG60E-Value: 0.000e+0, PID: 65.59(Putative transcriptional regulator SLK2 {ECO:0000... [more]
A0A5J5ATT5E-Value: 0.000e+0, PID: 66.81(Uncharacterized protein {ECO:0000313|EMBL:KAA8533... [more]
A0A5B7AJM8E-Value: 7.762e-31, PID: 67.01(Putative transcriptional regulator SLK2 {ECO:0000... [more]
A0A5B7AG29E-Value: 2.579e-30, PID: 64.39(Putative SEUSS-like 2 {ECO:0000313|EMBL:MPA55700.... [more]
A0A5J5BBV1E-Value: 4.673e-28, PID: 66.74(Uncharacterized protein {ECO:0000313|EMBL:KAA8539... [more]
A0A2K1YD61E-Value: 0.000e+0, PID: 64.66(Uncharacterized protein {ECO:0000313|EMBL:PNT1097... [more]
A0A5N5KPY6E-Value: 2.040e-26, PID: 63.54(Uncharacterized protein {ECO:0000313|EMBL:KAB5532... [more]
A0A4V6A988E-Value: 2.680e-25, PID: 64.32(Uncharacterized protein {ECO:0000313|EMBL:TKS0448... [more]
A0A837AV31E-Value: 1.758e-26, PID: 63.95(Transcriptional regulator SLK {ECO:0000313|EMBL:K... [more]
A0A835JP41E-Value: 8.066e-27, PID: 63.95(Uncharacterized protein {ECO:0000313|EMBL:KAF9671... [more]

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Analysis: 
NameDescription

An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4.

There are a few different naming schemes for this assembly. First there is the
Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below.
These sequences can be viewed in JBrowse here.

Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0

LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926.

Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence.

RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records.

The NCBI Sequence report lists the correspondences between the various naming methods

Link to the LNRQ01000000.1 master record at NCBI

Raw Reads: Link to SRA accessions used for the genome assembly

This genome is available in the CarrotOmics Blast Search

This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions.

For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins.

Data from this analysis can be viewed in JBrowse here.

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