DCAR_008908

Resource Type: 
Polypeptide
Name: 
DCAR_008908
Identifier: 
DCAR_008908.mRNA-protein
Sequence: 
MMHPKKLMKPSSRKPKKFLHKIVRVSVTDPDATDSSSDEEGGSFHKPRVK
KYVREIIVEATGVVSRRKVKENLKRVAAPAGRKFRGVRRRPWGKFAAEIR
DPVQRVRLWLGTFDTAEEAARVYDTAALRIRGPDALTNFIHPTEKPENKI
LDEISNVASPTSVLSLFEMEAEEYSKHENNYTSPCLTVDNSDVDHMSECQ
CREPFSFPNEPIFPNDDFSSQEYEIPSPFHEACLRYGFSFYQKTGIDDFD
IPIQLEEAGIYTDKDDSRIPYDLKLEIGSPLSSDVGDYFEENLSS*
Sequence Length: 
296
Sequence Checksum: 
8002335dea7e85f2d37a772cebcacf60
View location in JBrowse: 
Relationship: 
There is 1 relationship.
Relationships
The polypeptide, DCAR_008908, derives from mRNA, DCAR_008908.
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Blast Results: 
The following BLAST results are available for this feature:
BLAST of DCAR_008908 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
BLAST of DCAR_008908 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2R6Q955 ((Ethylene-responsive transcription factor {ECO:0000313|EMBL:PSS04425.1}))

HSP 1 Score: 180.259 bits (456), Expect = 5.257e-52
Identity = 119/303 (39.27%), Postives = 160/303 (52.81%), Query Frame = 0
 
Query:    5 KKLMKPSSRKPKK-------------FLHKIVRVSVTDPDATDSSSDEEGGSFHKPRVKKYVREIIVEATGVVSRRKVKENLKRVAAPAG---RKFRGVRRRPWGKFAAEIRDPVQRVRLWLGTFDTAEEAARVYDTAALRIRGPDALTNFIHPTEKPENKI-LDEIS---------NVASPTSVLSLFEMEAEEYSKHENNYTSPC------------------------LTVDNSDVDHMSECQCREPFSFPNEPIFPNDDFSSQEYEIPSPFHEACLRYGFS--------FYQKTGIDDF 249
            KKL+KPS+ KP+K                ++VR+SVTDPDATDSS DEE     + RVK+YV EI +E+    S  K ++ +K+ AA +G   +K+RGVRRRPWGKFAAEIRDP++R RLWLGT+DTAEEAA VYD AA+R+RGPDALTNF+ P  K    + +  +S         +++SPTSVL    +  EE    +     P                         L +D S VD     +  EP  F   P  PN D    +Y +   F ++   +G S        ++Q  G+D F
Sbjct:   21 KKLIKPSNLKPRKASGGPKVLDYTSGVAPRVVRISVTDPDATDSSGDEEAQFLGRQRVKRYVNEISIESAANKSGVK-RKGMKKAAAASGGGFQKYRGVRRRPWGKFAAEIRDPMRRTRLWLGTYDTAEEAAAVYDNAAIRLRGPDALTNFVTPPPKDAADVNMTSVSGYESGYESRSLSSPTSVLRFTSLSTEETKPSKPVQEDPVIPEPIQGSEEFQDETSLSTQIVDYLPMDLSLVDDFFNFESSEPILFDETPTLPN-DLLRDDY-LSDVFMDSGNDFGSSLSIWQANDYFQDLGVDLF 320    
BLAST of DCAR_008908 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5N6P685 (AP2/ERF domain-containing protein {ECO:0000259|PROSITE:PS51032})

HSP 1 Score: 178.718 bits (452), Expect = 4.416e-51
Identity = 128/314 (40.76%), Postives = 167/314 (53.18%), Query Frame = 0
 
Query:    6 KLMKPSSRKPKKFLHKIVRVSVTDPDATDSSSDEEGGSFHKPRVKKYVREIIVEATG--------------------VVSRRKVKENLKRVAAPA-GRKFRGVRRRPWGKFAAEIRDPVQRVRLWLGTFDTAEEAARVYDTAALRIRGPDALTNFIHPTEK---PENKI--------LDEISNVASPTSVLSLFEMEAEEYSKHE---------NNYTSPCLTVDNSDVDHMSECQCREPFSFPNEPIFPNDDFSSQEYEIPSPFHEACLRYGFSFYQKTGIDDFDI--PIQLEEAG----IYTDKDDSRIPYD 272
            K  K  +R+P+  + ++VR+S+TDPDATDSSSDEE   F + RVKKYV E+ ++ +G                    + +++KV ++      PA GRKFRGVR+RPWGK+AAEIRDP +RVRLWLGT+DTAEEAA VYD AA+++RGPDALTNF+ P  K   PEN +         DE  N  SPTSVL  FE E      HE         +N TSP    D   V+ +S  Q  +   F         D    + E+  PF    L   F +     IDDFD+  PI +E         TD   +  P+D
Sbjct:   11 KSKKHHNRQPEA-MPEVVRISMTDPDATDSSSDEEDELFVRRRVKKYVNEVKLQTSGKPAVAAVGCRKKFGKTGEGVLQAKQKVMKSTGATVLPANGRKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIKLRGPDALTNFVKPPAKETSPENSVPSTSGYESGDESINHPSPTSVLR-FESETNSKEDHEPVKEPDECQSNNTSPQEKPDWETVNVLSPVQNIQSGHF--------GDLGYDQLEMDIPF----LDNLFDYPPSNPIDDFDLPSPIHIENGAPSSEYATDDSFAATPFD 310    
BLAST of DCAR_008908 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A7J0DCR3 ((Cytokinin response factor 3 {ECO:0000313|EMBL:GFS32131.1}))

HSP 1 Score: 177.563 bits (449), Expect = 5.786e-51
Identity = 118/303 (38.94%), Postives = 160/303 (52.81%), Query Frame = 0
 
Query:    5 KKLMKPSSRKPKK-------------FLHKIVRVSVTDPDATDSSSDEEGGSFHKPRVKKYVREIIVEATGVVSRRKVKENLKRVAAPAG---RKFRGVRRRPWGKFAAEIRDPVQRVRLWLGTFDTAEEAARVYDTAALRIRGPDALTNFIHPTEKPENKI-LDEIS---------NVASPTSVLSLFEMEAEEYSKHENNYTSPC------------------------LTVDNSDVDHMSECQCREPFSFPNEPIFPNDDFSSQEYEIPSPFHEACLRYGFS--------FYQKTGIDDF 249
            KKL+KPS+ KP+K                ++VR+SVTDPDATDSS DEE     + RVK+YV EI +E+    S  K ++ +K+ AA +G   +K+RGVRRRPWGKFAAEIRDP++R RLWLGT+DTA+EAA VYD AA+R+RGPDALTNF+ P  K    + +  +S         +++SPTSVL    +  EE    +     P                         L +D S VD     +  EP  F   P  PN D    +Y +   F ++   +G S        ++Q  G+D F
Sbjct:   21 KKLIKPSNLKPRKASGGPKVLDYTSGVAPRVVRISVTDPDATDSSGDEEAQFLGRQRVKRYVNEISIESAANKSGVK-RKGMKKAAAASGGGFQKYRGVRRRPWGKFAAEIRDPMRRTRLWLGTYDTAKEAAAVYDNAAIRLRGPDALTNFVTPPPKDAADVNITSVSGYESGYESRSLSSPTSVLRFTSLSTEETEPSKPVQEDPVIPELIQGSEEFQDETSLSTQIVDYLPMDLSLVDDFFNFESSEPVLFDETPCLPN-DLLRDDY-LSDVFMDSGNDFGSSLSIWQADDYFQDLGVDLF 320    
BLAST of DCAR_008908 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A7J8V3C6 (AP2/ERF domain-containing protein {ECO:0000259|PROSITE:PS51032})

HSP 1 Score: 177.178 bits (448), Expect = 1.569e-50
Identity = 100/189 (52.91%), Postives = 121/189 (64.02%), Query Frame = 0
 
Query:   19 LHKIVRVSVTDPDATDSSSDEEGGSFHKPRVKKYVREI---IVEATGVVSRRKVKENLKRVAAPA----------------GRKFRGVRRRPWGKFAAEIRDPVQRVRLWLGTFDTAEEAARVYDTAALRIRGPDALTNFIHP-----TEKPENKILDEIS----------NVASPTSVLSLFEMEAEE 173
            + +IVRVSVTDPDATDSSSDE+   F + RVK+YV EI   +V A    +   V  N KR AA A                GRKFRGVR+RPWGK+AAEIRDP +RVRLWLGT+DTAEEAA VYD AA+++RGPDALTNF+ P      EKPEN  +  +S          N++SPTSVL+     +EE
Sbjct:   46 VRRIVRVSVTDPDATDSSSDEDADFFGRQRVKRYVNEINIEVVAAAVTTTSGAVNGNRKRTAAEAATGCRRPVKLSSTNGSGRKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIKLRGPDALTNFVTPPAKENNEKPENLNVSSVSGYESGDESAHNLSSPTSVLNFRTQSSEE 234    
BLAST of DCAR_008908 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A7J7CFB2 ((Putative Ethylene-responsive transcription factor {ECO:0000313|EMBL:KAF5732406.1}))

HSP 1 Score: 175.252 bits (443), Expect = 5.261e-50
Identity = 104/197 (52.79%), Postives = 124/197 (62.94%), Query Frame = 0
 
Query:    5 KKLMKPSSRKPKKF---------LHKIVRVSVTDPDATDSSSDEEGGSFHKPRVKKYVREIIVEATGVVSRRKVKENLKRVAAPA---------------GRKFRGVRRRPWGKFAAEIRDPVQRVRLWLGTFDTAEEAARVYDTAALRIRGPDALTNFIHP----TEKP-------ENKILDEISNVASPTSVLSL 166
            KK  KPS  KPKKF         + ++VR+SVTDPDATDSSSDEE   F + RVK+YV EI +EA+   S   V    KR AA                 GRKFRGVR+RPWGK+AAEIRDP +RVRLWLGT+DTAEEAA VYD AA+++RGPDALTNF+ P     EKP       E+    +  N+ SPTSVL+ 
Sbjct:   19 KKFRKPSF-KPKKFSDDRNPADQIPRVVRISVTDPDATDSSSDEEEDFFGRQRVKRYVNEINIEASCKASSTVVASGKKRSAAETLSHQPLKVQTTLTTNGRKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIKLRGPDALTNFVTPPARDEEKPSSIDSGYESADESQTHNLCSPTSVLNF 214    
BLAST of DCAR_008908 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A6J1G1E0 ((ethylene-responsive transcription factor CRF4-like {ECO:0000313|RefSeq:XP_022945589.1}))

HSP 1 Score: 174.096 bits (440), Expect = 1.094e-49
Identity = 135/325 (41.54%), Postives = 173/325 (53.23%), Query Frame = 0
 
Query:   11 SSRKPKKFLHKIVRVSVTDPDATDSSSDEEGGSFHKPRVKKYVREIIVE----------------ATGVVSRRKVKENLKRVAAPA--GRKFRGVRRRPWGKFAAEIRDPVQRVRLWLGTFDTAEEAARVYDTAALRIRGPDALTNF-IHPTEKPENKI---------------LDEISNVASPTSVLSLFEMEAEEYSKHENN--YTSPCLTVDNSDVDH-MSECQCREPFSFPNEPIFPNDDFSSQEYEIPSPFHEACLRYGFSFYQKTGIDDFDIPIQLEEAGIYTDKDDSRIPYDLKLEIGSPLS----SDVG-DYFEENL 293
            SSRK     +++VR++VTDPDATDSSSDEEG  F + RVK+YV EI ++                A G  SRR VK      AAP   G+KFRGVR+RPWGK+AAEIRDP +RVRLWLGT+DTAEEAA VYD AA+++RGPDALTNF I P   PE ++                +E  N++SP+SVL       EE  K +N+  +  P         DH + ECQ    F               +EY    P  E   +  FS   ++ +   D P+ LEE+ IY D        +L  + GSPL     S VG DYF++ L
Sbjct:   22 SSRKQPPSQNRVVRIAVTDPDATDSSSDEEGEFFERQRVKRYVNEIKIQTAPRNTLLPSARKRPAAEGSDSRRPVK------AAPVMNGKKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIPSVSGSYDDSCEESHNLSSPSSVLRFRTPSLEESEKPQNSSVFQKPPPPPLPPMADHQLRECQGETSF--------------YEEYYTDFPKFEDVFQLPFS---ESPMFLDDQPLYLEES-IYNDGFSDWF-TNLPDDFGSPLLSSSISQVGEDYFQDIL 321    
BLAST of DCAR_008908 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5D2WZX7 (AP2/ERF domain-containing protein {ECO:0000259|PROSITE:PS51032})

HSP 1 Score: 172.555 bits (436), Expect = 1.199e-49
Identity = 98/189 (51.85%), Postives = 120/189 (63.49%), Query Frame = 0
 
Query:   19 LHKIVRVSVTDPDATDSSSDEEGGSFHKPRVKKYVREIIVEATGVVSRRK---VKENLKRVAAPA----------------GRKFRGVRRRPWGKFAAEIRDPVQRVRLWLGTFDTAEEAARVYDTAALRIRGPDALTNFIHP-----TEKPENKILDEIS----------NVASPTSVLSLFEMEAEE 173
            + +IVRVSVTDPDATDSSSDE+   F + RVK+YV EI +E     +      +  N KR AA A                GRKFRGVR+RPWGK+AAEIRDP +RVRLWLGT+DTAEEAA VYD AA+++RGPDALTNF+ P      EKPEN  +  +S          N++SPTSVL+     +EE
Sbjct:   46 VRRIVRVSVTDPDATDSSSDEDADFFGRQRVKRYVNEINIEVVAAAATTTSGALNGNRKRTAAEAATGCRRPVKLPSTNGSGRKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIKLRGPDALTNFVTPPAKENNEKPENLNVSSVSGYESGDESAHNLSSPTSVLNFRTQSSEE 234    
BLAST of DCAR_008908 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5A7PNW1 ((Ethylene-responsive transcription factor {ECO:0000313|EMBL:GER34336.1}))

HSP 1 Score: 175.252 bits (443), Expect = 1.223e-49
Identity = 139/346 (40.17%), Postives = 176/346 (50.87%), Query Frame = 0
 
Query:    5 KKLMKPSSRKPKKFLHK------------IVRVSVTDPDATDSSSDEEGGSFHKPRVKKYVREIIVEATGVVSRRKVKENLKRVAAP---------------AGRKFRGVRRRPWGKFAAEIRDPVQRVRLWLGTFDTAEEAARVYDTAALRIRGPDALTNFIHPTEKPENK---------ILDEIS---NVASPTSVLSLFEMEAEEYSKHENNYTSPCLTVDNSDVDHMSECQ----CREPFSFPNEPIFPND---DFSSQEYE---IPSPFHEACLRYGFSFYQKTGIDDF------DIPIQLEEA-GIYTDKDDSRIPYD--LKLEIGSPLSSD-VGDYFEE 291
            KKL+KP  +KP+K   K             VR+SVTDPDATDSSSDEE   F + RVKKY+ EI +E+ G   R +  E+L+    P                 RKFRGVR+RPWGK+AAEIRDP +RVRLWLGT+DTAEEAA VYD+AA+++RGPDALTNF+ P+  PENK           DE S   N  SPTSVL      A   S  + + T  C     S V     C       EP   P+    PN    +F   E E   +P    +  L     F     +DDF      D P+  ++  G  T +D+ ++ +D    LEI  P   D V D F+E
Sbjct:   19 KKLIKPPKQKPRKQSPKSTGSSAAINGPRTVRISVTDPDATDSSSDEEDELFRRQRVKKYITEIRMESAGAACRTRASESLQARPKPMKSRTSPAPPSAADGGSRKFRGVRQRPWGKWAAEIRDPSRRVRLWLGTYDTAEEAAMVYDSAAIKLRGPDALTNFVTPS--PENKSSGAASGYSSGDESSHNRNHPSPTSVLRF----ATTRSGEDTDQTGLCSHSSESPVKGDEPCSGGFFTEEPVEEPSLRWNPNKLAREFEECEGETSTVPDYIVDDYLPMDIPF-----LDDFFNFQPQDGPVLFDDGPGFSTYQDEFKMCFDDFSALEIQGPSFGDVVHDSFQE 353    
BLAST of DCAR_008908 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A251SNY2 ((Putative AP2/ERF transcription factor ERF/PTI6 {ECO:0000313|EMBL:OTG00006.1}))

HSP 1 Score: 175.637 bits (444), Expect = 1.390e-49
Identity = 127/319 (39.81%), Postives = 169/319 (52.98%), Query Frame = 0
 
Query:    1 MMHPKKLMKPSSRKPKK--FLHKIVRVSVTDPDATDSSSDEEGGSFHKPRVKKYVREIIVEATGVV------------SRRKVKENLKRVAAPAG--RKFRGVRRRPWGKFAAEIRDPVQRVRLWLGTFDTAEEAARVYDTAALRIRGPDALTNFIHPTEK---PENKI--------LDEISNVASPTSVLSLFEMEAEEYSKHENNYTSPCLTVDNSDVDHMSECQ-CREPFSFPNEPIFPNDDFSSQEYEIPSPFHEACLRYGFSFYQKTGIDD-FDIPIQLEEAG---IYTDKDDSRIPYDLKLEIGSPLSSDVGD 287
            M+    L  P S+  ++   L K VR+S+TDPDATDSSSD+E   F + RVKKYV E+ ++ + VV            +++K  +     A PAG  RKFRGVR+RPWGK+AAEIRDP +RVRLWLGT+DTAEEAA VYD AA+++RGPDALTNF+ P  K   PE+ +         DE +N+ SPTSVL     E+E  SK E              ++ + E + C    S   E      D      +     H A L Y         +D+ FD P Q +E      Y D DD  +P  + +E G+P S  + D
Sbjct:   21 MVAVSSLTAPKSKNHRQPSSLPKTVRISMTDPDATDSSSDDEEELFGRRRVKKYVNEVNIQTSTVVKKVGRNGEGRLQAKQKAMKTAGAQAVPAGGVRKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIKLRGPDALTNFVTPPAKDSSPESNVPSTSGYESGDESNNLPSPTSVLGF---ESETTSKEE--------------LEPVKELEGCSSGCSSGQEIYGLQSDHDLHPVQDTESGHFADLVYDQLEMDVPFLDNLFDYPTQADEESPFLKYGDFDDIELPAPIHIENGAPSSEYITD 322    
BLAST of DCAR_008908 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5J5TKJ2 (AP2/ERF domain-containing protein {ECO:0000259|PROSITE:PS51032})

HSP 1 Score: 172.17 bits (435), Expect = 1.396e-49
Identity = 98/189 (51.85%), Postives = 120/189 (63.49%), Query Frame = 0
 
Query:   19 LHKIVRVSVTDPDATDSSSDEEGGSFHKPRVKKYVREIIVEATGVVSRRK---VKENLKRVAAPA----------------GRKFRGVRRRPWGKFAAEIRDPVQRVRLWLGTFDTAEEAARVYDTAALRIRGPDALTNFIHP-----TEKPENKILDEIS----------NVASPTSVLSLFEMEAEE 173
            + +IVRVSVTDPDATDSSSDE+   F + RVK+YV EI +E     +      +  N KR AA A                GRKFRGVR+RPWGK+AAEIRDP +RVRLWLGT+DTAEEAA VYD AA+++RGPDALTNF+ P      EKPEN  +  +S          N++SPTSVL+     +EE
Sbjct:   46 VRRIVRVSVTDPDATDSSSDEDADFFGRQRVKRYVNEINIEVVAAAATTTSGALNGNRKRTAAEAATGCRRPVKLPSTNGSGRKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIKLRGPDALTNFVTPPAKENNEKPENLNVSSVSGYESGDESAHNLSSPTSVLNFRTQSSEE 234    
Match NameStatsDescription
A0A2R6Q955E-Value: 5.257e-52, PID: 39.27(Ethylene-responsive transcription factor {ECO:000... [more]
A0A5N6P685E-Value: 4.416e-51, PID: 40.76AP2/ERF domain-containing protein {ECO:0000259|PRO... [more]
A0A7J0DCR3E-Value: 5.786e-51, PID: 38.94(Cytokinin response factor 3 {ECO:0000313|EMBL:GFS... [more]
A0A7J8V3C6E-Value: 1.569e-50, PID: 52.91AP2/ERF domain-containing protein {ECO:0000259|PRO... [more]
A0A7J7CFB2E-Value: 5.261e-50, PID: 52.79(Putative Ethylene-responsive transcription factor... [more]
A0A6J1G1E0E-Value: 1.094e-49, PID: 41.54(ethylene-responsive transcription factor CRF4-lik... [more]
A0A5D2WZX7E-Value: 1.199e-49, PID: 51.85AP2/ERF domain-containing protein {ECO:0000259|PRO... [more]
A0A5A7PNW1E-Value: 1.223e-49, PID: 40.17(Ethylene-responsive transcription factor {ECO:000... [more]
A0A251SNY2E-Value: 1.390e-49, PID: 39.81(Putative AP2/ERF transcription factor ERF/PTI6 {E... [more]
A0A5J5TKJ2E-Value: 1.396e-49, PID: 51.85AP2/ERF domain-containing protein {ECO:0000259|PRO... [more]

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Analysis: 
NameDescription

An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4.

There are a few different naming schemes for this assembly. First there is the
Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below.
These sequences can be viewed in JBrowse here.

Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0

LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926.

Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence.

RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records.

The NCBI Sequence report lists the correspondences between the various naming methods

Link to the LNRQ01000000.1 master record at NCBI

Raw Reads: Link to SRA accessions used for the genome assembly

This genome is available in the CarrotOmics Blast Search

This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions.

For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins.

Data from this analysis can be viewed in JBrowse here.

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