MATTLNLHLFPAQQFLQSKIQHNRRNPTSTFTKLNGSYRFSGIKCSVSNG
KLPNSTEEDEEEEKKKRIRAELSARIASGEFTVEKSGFQSQLLDGLAKLG
APSEVLDVLSKWIGASEDYPKIPEAKGAISAIRSEAFFIPLYELYLTYGG
VFRLTFGPKSFLIVSDPTVAKHILRDNSKAYSKGILAEILEFVMGKGLIP
ADGEIWRVRRRAIVPALHQKYVTAMISMFGQATDRLCAKLDAAASDAEDV
EMESLFSRLTLDIIGKAVFNYDFDSLTNDTGIVEAVYTVLREAEDRSVSP
IPFWEIPIWKDISPKLKKVNSALELINGTLDDLIAICKRMVDEEELQFHE
EYMNETDPTILVSSKQLRDDLMTMLIAGHETTAAVLTWTFYLLSKEPSVM
LKLQNEVDSVLGDRIPTIEDMKKLKYTTRVINESLRLYPQPPVLIRRSLE
DDKLGEYPIKRNEDIFISIWNLHRCPQRWEDADKFNPERWPLDGPNPNET
NQAFSYLPFGGGPRKCVGDMFASFEAIVAVAMLVRRFNFQMALGAPPVKM
TTGATIHTTEGLNMTVTKRITPPAVTAETSSLKDDSSVNMSREAVADQKG
EVSVART*
Relationships |
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The polypeptide, DCAR_023843, derives from mRNA, DCAR_023843. |
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
Match: A0A059P9X5 ((Chloroplast cytochrome P450 monooxygenase 97A3 {ECO:0000313|EMBL:AFU10536.1}))
HSP 1 Score: 1245.72 bits (3222), Expect = 0.000e+0
Identity = 606/616 (98.38%), Postives = 607/616 (98.54%), Query Frame = 0
Query: 1 MATTLNLHLFPAQQFLQSKIQHNRRNPTSTFTKLNGSYRFSGIKCSVSNGKLPNSTEEDEEEEKKKRIRAELSARIASGEFTVEKSGFQSQLLDGLAKLGAPSEVLDVLSKWIGASEDYPKIPEAKGAISAIRSEAFFIPLYELYLTYGGVFRLTFGPKSFLIVSDPTVAKHILRDNSKAYSKGILAEILEFVMGKGLIPADGEIWRVRRRAIVPALHQKYVTAMISMFGQATDRLCAKLDAAASDAEDVEMESLFSRLTLDIIGKAVFNYDFDSLTNDTGIVEAVYTVLREAEDRSVSPIPFWEIPIWKDISPKLKKVNSALELINGTLDDLIAICKRMVDEEELQFHEEYMNETDPTIL---------VSSKQLRDDLMTMLIAGHETTAAVLTWTFYLLSKEPSVMLKLQNEVDSVLGDRIPTIEDMKKLKYTTRVINESLRLYPQPPVLIRRSLEDDKLGEYPIKRNEDIFISIWNLHRCPQRWEDADKFNPERWPLDGPNPNETNQAFSYLPFGGGPRKCVGDMFASFEAIVAVAMLVRRFNFQMALGAPPVKMTTGATIHTTEGLNMTVTKRITPPAVTAETSSLKDDSSVNMSREAVADQKGEVSVART 607 MATTLNLHLFPAQQFLQSKIQHNRRNPTSTFTKLNGSYRFSGIKCSVSNGKLPNSTEEDEEEEKKKRIRAELSARIASGEFTVEKSGFQSQLLDGLAKLGAPSEVLDVLSKWIGASEDYPKIPEAKGAISAIRSEAFFIPLYELYLTYGGVFRLTFGPKSFLIVSDPTVAKHILRDNSKAYSKGILAEILEFVMGKGLIPADGEIWRVRRRAIVPALHQKYVTAMISMFGQATDRLCAKLDAAASDAEDVEMESLFSRLTLDIIGKAVFNYDFDSLTNDTGIVEAVYTVLREAEDRSVSPIPFWEIPIWKDISPKLKKVNSALELINGTLDDLIAICKRMVDEEELQFHEEYMNETDP+IL VSSKQLRDDLMTMLIAGHETTAAVLTWTFYLLSKEPSVMLKLQNEVDSVLGDRIPTIEDMKKLKYTTRVINESLRLYPQPPVLIRRSLEDDKLGEYPIKRNEDIFISIWNLHRCPQRWEDADKFNPERWPLDGPNPNETNQAFSYLPFGGGPRKCVGDMFASFEAIVAVAMLVRRFNFQMALGAPPVKMTTGATIHTTEGLNMTVTKRITPPAVTAETSSLKDDSSVNMSREAVADQKGEVSVART Sbjct: 1 MATTLNLHLFPAQQFLQSKIQHNRRNPTSTFTKLNGSYRFSGIKCSVSNGKLPNSTEEDEEEEKKKRIRAELSARIASGEFTVEKSGFQSQLLDGLAKLGAPSEVLDVLSKWIGASEDYPKIPEAKGAISAIRSEAFFIPLYELYLTYGGVFRLTFGPKSFLIVSDPTVAKHILRDNSKAYSKGILAEILEFVMGKGLIPADGEIWRVRRRAIVPALHQKYVTAMISMFGQATDRLCAKLDAAASDAEDVEMESLFSRLTLDIIGKAVFNYDFDSLTNDTGIVEAVYTVLREAEDRSVSPIPFWEIPIWKDISPKLKKVNSALELINGTLDDLIAICKRMVDEEELQFHEEYMNETDPSILHFLLASGDDVSSKQLRDDLMTMLIAGHETTAAVLTWTFYLLSKEPSVMLKLQNEVDSVLGDRIPTIEDMKKLKYTTRVINESLRLYPQPPVLIRRSLEDDKLGEYPIKRNEDIFISIWNLHRCPQRWEDADKFNPERWPLDGPNPNETNQAFSYLPFGGGPRKCVGDMFASFEAIVAVAMLVRRFNFQMALGAPPVKMTTGATIHTTEGLNMTVTKRITPPAVTAETSSLKDDSSVNMSREAVADQKGEVSVART 616
Match: A0A5N5HWU8 ((Protein LUTEIN DEFICIENT 5 {ECO:0000313|EMBL:KAB2629960.1}))
HSP 1 Score: 959.133 bits (2478), Expect = 0.000e+0
Identity = 470/619 (75.93%), Postives = 525/619 (84.81%), Query Frame = 0
Query: 2 ATTLNLHLFPAQQFLQSKIQHNRRNPTSTFTKLNGSYRFSGIKCSVSNGKLPNSTE---EDEEEEKKKRIRAELSARIASGEFTVEKSGFQSQLLDGLAKLGAPSEVLDVLSKWIGASEDYPKIPEAKGAISAIRSEAFFIPLYELYLTYGGVFRLTFGPKSFLIVSDPTVAKHILRDNSKAYSKGILAEILEFVMGKGLIPADGEIWRVRRRAIVPALHQKYVTAMISMFGQATDRLCAKLDAAASDAEDVEMESLFSRLTLDIIGKAVFNYDFDSLTNDTGIVEAVYTVLREAEDRSVSPIPFWEIPIWKDISPKLKKVNSALELINGTLDDLIAICKRMVDEEELQFHEEYMNETDPTIL---------VSSKQLRDDLMTMLIAGHETTAAVLTWTFYLLSKEPSVMLKLQNEVDSVLGDRIPTIEDMKKLKYTTRVINESLRLYPQPPVLIRRSLEDDKLGEYPIKRNEDIFISIWNLHRCPQRWEDADKFNPERWPLDGPNPNETNQAFSYLPFGGGPRKCVGDMFASFEAIVAVAMLVRRFNFQMALGAPPVKMTTGATIHTTEGLNMTVTKRITPPAV-TAETSSLKDDSSVNMSR-EAVADQKGEVSVAR 606 AT L PA+ F Q+K NR PT K GS+ S I C+ SNG+ P+S + + E +++ RAELSARI+SGEFTVEKSGF SQL GL+K+G P+ VLD L W A E+YPKIPEAKGAISAI+SEAFF+PL+ELYLTYGG++RLTFGPKSFLIVSDP +AKHILR+NSKAYSKGILAEILEFVMG GLIPADGEIWR+RRRAIVPALHQKYVTAMI++FG+ATDRLC KLDAAASD EDVEMESLFSRLTLDIIGKA+FNYDFDSLTNDTGIVEAVYTVLREAEDRSV+PIP WEIPIWKDISP+ +KV +AL+LIN TLDDLIAICKRMVDEEELQFHEEYMNE DP+IL VSSKQLRDDLMTMLIAGHET+AAVLTWTFYLLS EP VM KLQ E DSVLGDR PTI+DMKKLKY TRVINESLRLYPQPPVLIRRSLEDDKLG+YPIKRNEDIFI IWNLHR P++W+DADKFNPERWPLDGPNPNETNQ F YLPFGGGPRKC+GDMFA+FE +VA++MLVRRFNFQMALGAP VKMTTGATIHTTEGL MTV++RI PP V T E + + D+ V + + +++ QKGEVS A Sbjct: 599 ATIPLLQFVPAKTF-QAKHHSNRLRPT----KQRGSFGLSMIICASSNGREPDSADGGVKSVERLLEEKRRAELSARISSGEFTVEKSGFPSQLKSGLSKVGVPTGVLDFLFSWADAPENYPKIPEAKGAISAIQSEAFFVPLFELYLTYGGIYRLTFGPKSFLIVSDPAIAKHILRENSKAYSKGILAEILEFVMGTGLIPADGEIWRLRRRAIVPALHQKYVTAMINLFGEATDRLCQKLDAAASDGEDVEMESLFSRLTLDIIGKALFNYDFDSLTNDTGIVEAVYTVLREAEDRSVAPIPLWEIPIWKDISPRQRKVATALKLINTTLDDLIAICKRMVDEEELQFHEEYMNEQDPSILHFLLASGDDVSSKQLRDDLMTMLIAGHETSAAVLTWTFYLLSTEPGVMAKLQEEADSVLGDRFPTIDDMKKLKYATRVINESLRLYPQPPVLIRRSLEDDKLGDYPIKRNEDIFICIWNLHRSPKQWDDADKFNPERWPLDGPNPNETNQNFRYLPFGGGPRKCLGDMFATFETVVALSMLVRRFNFQMALGAPEVKMTTGATIHTTEGLKMTVSRRIKPPIVPTLEMPAFEVDTPVGVPKGDSLVSQKGEVSSAH 1212
Match: A0A2C9VBY7 ((Uncharacterized protein {ECO:0000313|EMBL:OAY42483.1}))
HSP 1 Score: 956.822 bits (2472), Expect = 0.000e+0
Identity = 474/612 (77.45%), Postives = 524/612 (85.62%), Query Frame = 0
Query: 16 LQSKIQHNRR---NPTSTFTKLNGSYRFSGIKCSVSNGKLPNSTE------EDEEEEKKKRIRAELSARIASGEFTVEKSGFQSQLLDGLAKLGAPSEVLDVLSKWIGASEDYPKIPEAKGAISAIRSEAFFIPLYELYLTYGGVFRLTFGPKSFLIVSDPTVAKHILRDNSKAYSKGILAEILEFVMGKGLIPADGEIWRVRRRAIVPALHQKYVTAMISMFGQATDRLCAKLDAAASDAEDVEMESLFSRLTLDIIGKAVFNYDFDSLTNDTGIVEAVYTVLREAEDRSVSPIPFWEIPIWKDISPKLKKVNSALELINGTLDDLIAICKRMVDEEELQFHEEYMNETDPTIL---------VSSKQLRDDLMTMLIAGHETTAAVLTWTFYLLSKEPSVMLKLQNEVDSVLGDRIPTIEDMKKLKYTTRVINESLRLYPQPPVLIRRSLEDDKLGEYPIKRNEDIFISIWNLHRCPQRWEDADKFNPERWPLDGPNPNETNQAFSYLPFGGGPRKCVGDMFASFEAIVAVAMLVRRFNFQMALGAPPVKMTTGATIHTTEGLNMTVTKRITPPAVTA-ETSSLKDDSSVNM-SREAVADQKGEVSVART 607 QSK+Q + NP + + NG +FS I C+ SNG+ P S+E E EEK+ RAELSARIASGEFTV+ SGF S L +GL+K+G P+E+L+ L KW+ A EDY KIPEAKGAISAIRSEAFFIPLYELYLTYGG+FRLTFGPKSFLIVSDP++AKHIL+DNSKAYSKGILAEIL+FVMGKGLIPADGE+WRVRRRAIVPALHQKYV AMI +FGQATDRLC KLDAAASD EDVEMESLFSRLTLDIIGKAVFNY+FDSLTNDTGIVEAVYTVLREAEDRSV+PIPFWEIP+WKDISP+ +KV++AL+LIN LD+LIAICKRMVDEEEL FHEEYMNE DP+IL VSSKQLRDDLMTMLIAGHET+AAVLTWTFYLLSKEPSV+ KLQ+EVDSVLGDR PTIEDMKKLKYTTRVINESLRLYPQPPVLIRRSLE+D LG+YPI R EDIFIS+WNLHR P WEDA+KFNPERWPLDGPNPNETNQ F YLPFGGGPRKCVGDMFASFE++VAVAMLVRRFNFQMALGAPPVKMTTGATIHTTEGL MTVT RI PP + +T ++ D+ VN+ S E QKGEVS A + Sbjct: 23 FQSKLQLKKFKPINPCLSPSIQNGKCKFSVISCASSNGREPESSEGGIKGVEKILEEKR---RAELSARIASGEFTVKNSGFPSILKNGLSKIGVPNEILEFLFKWVDAGEDYSKIPEAKGAISAIRSEAFFIPLYELYLTYGGIFRLTFGPKSFLIVSDPSIAKHILKDNSKAYSKGILAEILDFVMGKGLIPADGEVWRVRRRAIVPALHQKYVAAMIRLFGQATDRLCKKLDAAASDGEDVEMESLFSRLTLDIIGKAVFNYEFDSLTNDTGIVEAVYTVLREAEDRSVAPIPFWEIPVWKDISPRQRKVSAALKLINEILDELIAICKRMVDEEELNFHEEYMNEQDPSILHFLLASGDDVSSKQLRDDLMTMLIAGHETSAAVLTWTFYLLSKEPSVLSKLQSEVDSVLGDRFPTIEDMKKLKYTTRVINESLRLYPQPPVLIRRSLENDVLGKYPITRGEDIFISVWNLHRSPHLWEDAEKFNPERWPLDGPNPNETNQNFCYLPFGGGPRKCVGDMFASFESVVAVAMLVRRFNFQMALGAPPVKMTTGATIHTTEGLKMTVTPRIKPPIIPKLDTPAVTVDAPVNVSSEEPQLGQKGEVSAAHS 631
Match: A0A1S4CL37 ((protein LUTEIN DEFICIENT 5, chloroplastic-like {ECO:0000313|RefSeq:XP_016501972.1}))
HSP 1 Score: 955.281 bits (2468), Expect = 0.000e+0
Identity = 470/620 (75.81%), Postives = 531/620 (85.65%), Query Frame = 0
Query: 1 MATTLNLHLFPAQQFLQSKIQHNRRNPTSTFTKLNGSYRFSG-IKCSVSNGKLPNSTEEDEEEEKK---KRIRAELSARIASGEFTVEKSGFQSQLLDGLAKLGAPSEVLDVLSKWIGASEDYPKIPEAKGAISAIRSEAFFIPLYELYLTYGGVFRLTFGPKSFLIVSDPTVAKHILRDNSKAYSKGILAEILEFVMGKGLIPADGEIWRVRRRAIVPALHQKYVTAMISMFGQATDRLCAKLDAAASDAEDVEMESLFSRLTLDIIGKAVFNYDFDSLTNDTGIVEAVYTVLREAEDRSVSPIPFWEIPIWKDISPKLKKVNSALELINGTLDDLIAICKRMVDEEELQFHEEYMNETDPTIL---------VSSKQLRDDLMTMLIAGHETTAAVLTWTFYLLSKEPSVMLKLQNEVDSVLGDRIPTIEDMKKLKYTTRVINESLRLYPQPPVLIRRSLEDDKLGEYPIKRNEDIFISIWNLHRCPQRWEDADKFNPERWPLDGPNPNETNQAFSYLPFGGGPRKCVGDMFASFEAIVAVAMLVRRFNFQMALGAPPVKMTTGATIHTTEGLNMTVTKRITPPAV-TAETSSLKDDSSVNMSR-EAVADQKGEVSVA 605 MA ++ L FP ++ + +R PT++ K NG+Y + I+C+ SNG+ P S E + +K ++ RAELSARIASGEFTVE++GF S + +GL+KLG P E S+W+G DYP+IPEAKG+ISA+R+EAFFIPLYEL+LTYGG+FRLTFGPKSFLIVSDP++AKHIL+DNSKAYSKGILAEIL+FVMGKGLIPADGEIWRVRRRAIVPALHQKYV AMI +FGQATDRLC KLDAAASD EDVEMESLFSRLTLDIIGKAVFNYDFDSLT DTGIVEAVYTVLREAEDRSV+PIP WEIPIWKDISPKLKKVN+AL+LIN TLDDLIA+CKRMVDEEELQFHEEYMNE DP+IL VSSKQLRDDLMTMLIAGHET+AAVLTWTFYLLSKEPSVM KLQ+EVDSVLGDR PTIED+KKL+YTTRVINESLRLYPQPPVLIRRS+++D LG YPIKR EDIFIS+WNLHRCP WE+ADKFNPERWPLDGPNPNETNQ +SYLPFGGGPRKCVGDMFA+FE IVAVAMLVRRF+FQMALGAPPVKMTTGATIHTTEGLNMTVT+R PP V E ++L+ DSSVN S +V++Q+ E + Sbjct: 1 MAASVPLLQFPTHSLHKTTL--SRFRPTTS-PKFNGNYGYCCTIRCNSSNGREPESVNEGVKTVEKLLEEKRRAELSARIASGEFTVEQTGFPSLVKNGLSKLGVPKEFFQFFSQWLG---DYPRIPEAKGSISAVRNEAFFIPLYELFLTYGGIFRLTFGPKSFLIVSDPSIAKHILKDNSKAYSKGILAEILDFVMGKGLIPADGEIWRVRRRAIVPALHQKYVAAMIGLFGQATDRLCKKLDAAASDGEDVEMESLFSRLTLDIIGKAVFNYDFDSLTVDTGIVEAVYTVLREAEDRSVAPIPVWEIPIWKDISPKLKKVNTALKLINDTLDDLIALCKRMVDEEELQFHEEYMNEKDPSILHFLLASGDEVSSKQLRDDLMTMLIAGHETSAAVLTWTFYLLSKEPSVMAKLQDEVDSVLGDRFPTIEDLKKLRYTTRVINESLRLYPQPPVLIRRSIDEDVLGGYPIKRGEDIFISVWNLHRCPNHWEEADKFNPERWPLDGPNPNETNQNYSYLPFGGGPRKCVGDMFATFENIVAVAMLVRRFDFQMALGAPPVKMTTGATIHTTEGLNMTVTRRTRPPVVPNLEMATLEVDSSVNSSDINSVSNQRAETEAS 614
Match: A0A1U7YTG1 ((protein LUTEIN DEFICIENT 5, chloroplastic {ECO:0000313|RefSeq:XP_009802494.1}))
HSP 1 Score: 954.51 bits (2466), Expect = 0.000e+0
Identity = 469/620 (75.65%), Postives = 531/620 (85.65%), Query Frame = 0
Query: 1 MATTLNLHLFPAQQFLQSKIQHNRRNPTSTFTKLNGSYRFSG-IKCSVSNGKLPNSTEEDEEEEKK---KRIRAELSARIASGEFTVEKSGFQSQLLDGLAKLGAPSEVLDVLSKWIGASEDYPKIPEAKGAISAIRSEAFFIPLYELYLTYGGVFRLTFGPKSFLIVSDPTVAKHILRDNSKAYSKGILAEILEFVMGKGLIPADGEIWRVRRRAIVPALHQKYVTAMISMFGQATDRLCAKLDAAASDAEDVEMESLFSRLTLDIIGKAVFNYDFDSLTNDTGIVEAVYTVLREAEDRSVSPIPFWEIPIWKDISPKLKKVNSALELINGTLDDLIAICKRMVDEEELQFHEEYMNETDPTIL---------VSSKQLRDDLMTMLIAGHETTAAVLTWTFYLLSKEPSVMLKLQNEVDSVLGDRIPTIEDMKKLKYTTRVINESLRLYPQPPVLIRRSLEDDKLGEYPIKRNEDIFISIWNLHRCPQRWEDADKFNPERWPLDGPNPNETNQAFSYLPFGGGPRKCVGDMFASFEAIVAVAMLVRRFNFQMALGAPPVKMTTGATIHTTEGLNMTVTKRITPPAV-TAETSSLKDDSSVNMSR-EAVADQKGEVSVA 605 MA ++ L FP ++ + +R PT++ K NG++ + I+CS SNG+ P S E + +K ++ RAELSARIASGEFTVE++GF S + +GL+KLG P E S+W+G DYP+IPEAKG+ISA+R+EAFFIPLYEL+LTYGG+FRLTFGPKSFLIVSDP++AKHIL+DNSKAYSKGILAEIL+FVMGKGLIPADGEIWRVRRRAIVPALHQKYV AMI +FGQATDRLC KLDAAASD EDVEMESLFSRLTLDIIGKAVFNYDFDSLT DTGIVEAVYTVLREAEDRSV+PIP WEIPIWKDISPKLKKVN+AL+LIN TLDDLIA+CKRMVDEEELQFHEEYMNE DP+IL VSSKQLRDDLMTMLIAGHET+AAVLTWTFYLLSKEPSVM KLQ+EVDSVLGDR PTIED+KKL+YTTRVINESLRLYPQPPVLIRRS+++D LG YPIKR EDIFIS+WNLHRCP WE+ADKFNPERWPLDGPNPNETNQ +SYLPFGGGPRKCVGDMFA+FE +VAVAMLVRRF+FQMALGAPPVKMTTGATIHTTEGLNMTVT+R PP V E ++L+ DSSVN S +V++Q+ E + Sbjct: 1 MAASVPLLQFPTHSLHKTTL--SRFRPTTS-PKFNGNFGYCCTIRCSSSNGREPESVNEGVKTVEKLLEEKRRAELSARIASGEFTVEQTGFPSLVKNGLSKLGVPKEFFQFFSQWLG---DYPRIPEAKGSISAVRNEAFFIPLYELFLTYGGIFRLTFGPKSFLIVSDPSIAKHILKDNSKAYSKGILAEILDFVMGKGLIPADGEIWRVRRRAIVPALHQKYVAAMIGLFGQATDRLCKKLDAAASDGEDVEMESLFSRLTLDIIGKAVFNYDFDSLTVDTGIVEAVYTVLREAEDRSVAPIPVWEIPIWKDISPKLKKVNTALKLINDTLDDLIALCKRMVDEEELQFHEEYMNEKDPSILHFLLASGDEVSSKQLRDDLMTMLIAGHETSAAVLTWTFYLLSKEPSVMAKLQDEVDSVLGDRFPTIEDLKKLRYTTRVINESLRLYPQPPVLIRRSIDEDVLGGYPIKRGEDIFISVWNLHRCPNHWEEADKFNPERWPLDGPNPNETNQNYSYLPFGGGPRKCVGDMFATFENVVAVAMLVRRFDFQMALGAPPVKMTTGATIHTTEGLNMTVTRRTRPPVVPNLEMATLEVDSSVNSSDINSVSNQRAETEAS 614
Match: A0A314LDR6 ((Protein lutein deficient 5, chloroplastic {ECO:0000313|EMBL:OIT39795.1}))
HSP 1 Score: 953.355 bits (2463), Expect = 0.000e+0
Identity = 468/620 (75.48%), Postives = 531/620 (85.65%), Query Frame = 0
Query: 1 MATTLNLHLFPAQQFLQSKIQHNRRNPTSTFTKLNGSYRFSG-IKCSVSNGKLPNSTEEDEEEEKK---KRIRAELSARIASGEFTVEKSGFQSQLLDGLAKLGAPSEVLDVLSKWIGASEDYPKIPEAKGAISAIRSEAFFIPLYELYLTYGGVFRLTFGPKSFLIVSDPTVAKHILRDNSKAYSKGILAEILEFVMGKGLIPADGEIWRVRRRAIVPALHQKYVTAMISMFGQATDRLCAKLDAAASDAEDVEMESLFSRLTLDIIGKAVFNYDFDSLTNDTGIVEAVYTVLREAEDRSVSPIPFWEIPIWKDISPKLKKVNSALELINGTLDDLIAICKRMVDEEELQFHEEYMNETDPTIL---------VSSKQLRDDLMTMLIAGHETTAAVLTWTFYLLSKEPSVMLKLQNEVDSVLGDRIPTIEDMKKLKYTTRVINESLRLYPQPPVLIRRSLEDDKLGEYPIKRNEDIFISIWNLHRCPQRWEDADKFNPERWPLDGPNPNETNQAFSYLPFGGGPRKCVGDMFASFEAIVAVAMLVRRFNFQMALGAPPVKMTTGATIHTTEGLNMTVTKRITPPAVTA-ETSSLKDDSSVNMSR-EAVADQKGEVSVA 605 MA ++ L FP ++ + +R PT++ K NG+Y + I+C+ SNG+ P S E + +K ++ RAELSARIASGEFTVE++GF S + +GL+KLG P E S+W+G DYP+IPEAKG+ISA+R+EAFFIPLYEL+LTYGG+FRLTFGPKSFLIVSDP++AKHIL+DNSKAYSKGILAEIL+FVMGKGLIPADGEIWRVRRRAIVPALHQKYV AMI +FGQATDRLC KLDAAASD EDVEMESLFSRLTLDIIGKAVFNYDFDSLT DTGIVEAVYTVLREAEDRSV+PIP WEIPIWKDISPKLKKVN+AL+LIN TLDDLIA+CKRMVDEEELQFHEEYMNE DP+IL VSSKQLRDDLMTMLIAGHET+AAVLTWTFYLLSKEPSVM KLQ+EVDSVLGDR PTIED+KKL+YTTRVINESLRLYPQPPVLIRRS+++D LG YPIKR EDIFIS+WNLHRCP WE+ADKFNPERWPLDGPNPNETNQ +SYLPFGGGPRKCVGDMFA+FE +VAVAMLVRRF+FQMA+GAPPVKMTTGATIHTTEGLNMTVT+R PP V E ++L+ DSSVN S +V++Q+ E + Sbjct: 1 MAASVPLLQFPTHSLHKTTL--SRFRPTTS-PKFNGNYGYCCTIRCNSSNGREPESVNEGVKTVEKLLEEKRRAELSARIASGEFTVEQTGFPSLVKNGLSKLGVPKEFFQFFSQWLG---DYPRIPEAKGSISAVRNEAFFIPLYELFLTYGGIFRLTFGPKSFLIVSDPSIAKHILKDNSKAYSKGILAEILDFVMGKGLIPADGEIWRVRRRAIVPALHQKYVAAMIGLFGQATDRLCKKLDAAASDGEDVEMESLFSRLTLDIIGKAVFNYDFDSLTVDTGIVEAVYTVLREAEDRSVAPIPVWEIPIWKDISPKLKKVNTALKLINDTLDDLIALCKRMVDEEELQFHEEYMNEKDPSILHFLLASGDEVSSKQLRDDLMTMLIAGHETSAAVLTWTFYLLSKEPSVMAKLQDEVDSVLGDRFPTIEDLKKLRYTTRVINESLRLYPQPPVLIRRSIDEDVLGGYPIKRGEDIFISVWNLHRCPNHWEEADKFNPERWPLDGPNPNETNQNYSYLPFGGGPRKCVGDMFATFENVVAVAMLVRRFDFQMAIGAPPVKMTTGATIHTTEGLNMTVTRRTRPPVVPKLEMATLEIDSSVNSSDINSVSNQRAEAEAS 614
Match: A0A059AXX5 ((Uncharacterized protein {ECO:0000313|EMBL:KCW58728.1}))
HSP 1 Score: 952.584 bits (2461), Expect = 0.000e+0
Identity = 466/609 (76.52%), Postives = 526/609 (86.37%), Query Frame = 0
Query: 16 LQSKIQHNR-RNPTSTFTKLNGSYRF-SGIKCSVSNGKLPNSTEE----DEEEEKKKRIRAELSARIASGEFTVEKSGFQSQLLDGLAKLGAPSEVLDVLSKWIGASEDYPKIPEAKGAISAIRSEAFFIPLYELYLTYGGVFRLTFGPKSFLIVSDPTVAKHILRDNSKAYSKGILAEILEFVMGKGLIPADGEIWRVRRRAIVPALHQKYVTAMISMFGQATDRLCAKLDAAASDAEDVEMESLFSRLTLDIIGKAVFNYDFDSLTNDTGIVEAVYTVLREAEDRSVSPIPFWEIPIWKDISPKLKKVNSALELINGTLDDLIAICKRMVDEEELQFHEEYMNETDPTIL---------VSSKQLRDDLMTMLIAGHETTAAVLTWTFYLLSKEPSVMLKLQNEVDSVLGDRIPTIEDMKKLKYTTRVINESLRLYPQPPVLIRRSLEDDKLGEYPIKRNEDIFISIWNLHRCPQRWEDADKFNPERWPLDGPNPNETNQAFSYLPFGGGPRKCVGDMFASFEAIVAVAMLVRRFNFQMALGAPPVKMTTGATIHTTEGLNMTVTKRITPPAV-TAETSSLKDDSSVNMSR-EAVADQKGEVSVART 607 Q+K++ R R P S KL G+YR+ + I C+ SNG+ P S ++ E+ +++ RAELSARIA+GEFT+E+SGF SQ+ +GL+K+G P EVLD +W +S+D+PKIPEAKGAI AI++EAFFIPLYELYLTYGG+FRLTFGPKSFLIVSDP++AKHIL+DNSKAYSKGILAEILEFVMGKGLIPADGE+WRVRRR IVPALHQKYV AMI++FGQ T RLC KLD AAS+ EDVEMESLFSRLTLD+IGKAVFNYDFDSLTNDTGI+EAVYTVLREAEDRSVSPIPFWEIPIWKDISPK KKVN AL+L+N L+DLIAICKRMVDEE+LQFHEEYMNE DP+IL VSSKQLRDDLMTMLIAGHET+AAVLTWTFYLLSKEP+VM KLQNEVDSVLGDRIPTIEDMKKLKYTTRVINES+RLYPQPPVLIRRSLE+D LG+YPIKR EDIFIS+WNLHR P W DADKFNPERWPLDGPNPNETNQ FSYLPFGGGPRKCVGDMFASFE +VAVAMLVRRFNFQMALGAP VKMTTGATIHTTEGL MTVT+R+ PP V T E L+ ++S + S+ ++V QKGEV+ A + Sbjct: 20 FQTKLRTTRFRQPASA--KLKGNYRWATAIACASSNGREPESVDDRGVKSAEQLLEEKRRAELSARIAAGEFTIERSGFPSQVRNGLSKVGVPPEVLDFFFQWFDSSKDHPKIPEAKGAIKAIQNEAFFIPLYELYLTYGGIFRLTFGPKSFLIVSDPSIAKHILKDNSKAYSKGILAEILEFVMGKGLIPADGEVWRVRRRTIVPALHQKYVAAMINLFGQTTQRLCDKLDIAASEREDVEMESLFSRLTLDVIGKAVFNYDFDSLTNDTGIIEAVYTVLREAEDRSVSPIPFWEIPIWKDISPKQKKVNEALKLVNDALNDLIAICKRMVDEEDLQFHEEYMNEEDPSILHFLLASGDDVSSKQLRDDLMTMLIAGHETSAAVLTWTFYLLSKEPTVMSKLQNEVDSVLGDRIPTIEDMKKLKYTTRVINESMRLYPQPPVLIRRSLENDFLGKYPIKRGEDIFISVWNLHRSPHHWIDADKFNPERWPLDGPNPNETNQNFSYLPFGGGPRKCVGDMFASFEMVVAVAMLVRRFNFQMALGAPAVKMTTGATIHTTEGLKMTVTRRLQPPIVPTLEMPLLQVENSASSSQGDSVLSQKGEVTSAHS 626
Match: A0A540KET6 ((Uncharacterized protein {ECO:0000313|EMBL:TQD72744.1}))
HSP 1 Score: 950.658 bits (2456), Expect = 0.000e+0
Identity = 475/622 (76.37%), Postives = 522/622 (83.92%), Query Frame = 0
Query: 2 ATTLNLHLFPAQQFLQSKIQHNRRNPTSTFTKLNGSYRFSGIKCSVSNGKLPNSTE------EDEEEEKKKRIRAELSARIASGEFTVEKSGFQSQLLDGLAKLGAPSEVLDVLSKWIGASEDYPKIPEAKGAISAIRSEAFFIPLYELYLTYGGVFRLTFGPKSFLIVSDPTVAKHILRDNSKAYSKGILAEILEFVMGKGLIPADGEIWRVRRRAIVPALHQKYVTAMISMFGQATDRLCAKLDAAASDAEDVEMESLFSRLTLDIIGKAVFNYDFDSLTNDTGIVEAVYTVLREAEDRSVSPIPFWEIPIWKDISPKLKKVNSALELINGTLDDLIAICKRMVDEEELQFHEEYMNETDPTIL---------VSSKQLRDDLMTMLIAGHETTAAVLTWTFYLLSKEPSVMLKLQNEVDSVLGDRIPTIEDMKKLKYTTRVINESLRLYPQPPVLIRRSLEDDKLGEYPIKRNEDIFISIWNLHRCPQRWEDADKFNPERWPLDGPNPNETNQAFSYLPFGGGPRKCVGDMFASFEAIVAVAMLVRRFNFQMALGAPPVKMTTGATIHTTEGLNMTVTKRITPPAV-TAETSSLKDDSSVNMSR-EAVADQKGEVSVAR 606 A T+ L F + LQ+K+ NR P K G + S I CS SNG+ P+S + E EEK+ RAELSA IASGEFTVEKSGF SQL GL+K+G P+ +LD L W A EDYPKIPEAKGAISAIRSEAFFIPL+ELYLTYGG++RL FGPKSFLIVSDP +AKHILR+NSKAYSKGILAEILEFVMG GLIPADGEIWR RRRAIVPALHQKYVTAMI++FG+ATDRLC KLDAAASD EDVEMESLFSRLTLDIIGKA+FNYDFDSLTNDTGIVEAVYTVLRE+EDRSVSPIP WEIPIWKDISP+ +KV++AL+LIN LDDLIAICKRMVDEEELQFHEEYMNE DP+IL VSSKQLRDDLMTMLIAGHET+AAVLTWTFYLLSKEP VM KLQ EVDSVLGDR PTIEDMKKLKY TRVINESLRLYPQPPVLIRRSLEDDKLG++PIKRNEDIFI IWNLHR P++W+DADKF PERWPLDGPNPNETNQ F YLPFGGGPRKCVGDMFA+FE +VA++MLVRRFNFQ+ALGAP VKMTTGATIHTTEGL MTVT+RI PP V T E S + D+ V + + ++V QKGEVS A Sbjct: 60 AATIPLLQFVPAKTLQAKLHSNRLRPI----KQRGRFGLSTITCSSSNGREPDSVDIGVKSVERLLEEKR---RAELSAGIASGEFTVEKSGFPSQLKSGLSKVGVPTGILDFLFSWADAPEDYPKIPEAKGAISAIRSEAFFIPLFELYLTYGGIYRLKFGPKSFLIVSDPAIAKHILRENSKAYSKGILAEILEFVMGTGLIPADGEIWRRRRRAIVPALHQKYVTAMINLFGEATDRLCQKLDAAASDGEDVEMESLFSRLTLDIIGKALFNYDFDSLTNDTGIVEAVYTVLRESEDRSVSPIPVWEIPIWKDISPRQRKVSTALKLINTALDDLIAICKRMVDEEELQFHEEYMNEQDPSILHFLLASGDDVSSKQLRDDLMTMLIAGHETSAAVLTWTFYLLSKEPRVMAKLQEEVDSVLGDRFPTIEDMKKLKYATRVINESLRLYPQPPVLIRRSLEDDKLGDFPIKRNEDIFICIWNLHRSPKQWDDADKFYPERWPLDGPNPNETNQNFRYLPFGGGPRKCVGDMFATFETVVALSMLVRRFNFQLALGAPEVKMTTGATIHTTEGLKMTVTRRIKPPIVPTLEMPSFEVDTPVGIPKGDSVVGQKGEVSSAH 674
Match: A0A251P439 ((Uncharacterized protein {ECO:0000313|EMBL:ONI05135.1}))
HSP 1 Score: 948.347 bits (2450), Expect = 0.000e+0
Identity = 464/604 (76.82%), Postives = 516/604 (85.43%), Query Frame = 0
Query: 1 MATTLNLHLFPAQQFLQSKIQHNRRNPTSTFTKLNGSYRFSGIKCSVSNGKLPNSTEEDEEEEK--KKRIRAELSARIASGEFTVEKSGFQSQLLDGLAKLGAPSEVLDVLSKWIGASEDYPKIPEAKGAISAIRSEAFFIPLYELYLTYGGVFRLTFGPKSFLIVSDPTVAKHILRDNSKAYSKGILAEILEFVMGKGLIPADGEIWRVRRRAIVPALHQKYVTAMISMFGQATDRLCAKLDAAASDAEDVEMESLFSRLTLDIIGKAVFNYDFDSLTNDTGIVEAVYTVLREAEDRSVSPIPFWEIPIWKDISPKLKKVNSALELINGTLDDLIAICKRMVDEEELQFHEEYMNETDPTIL---------VSSKQLRDDLMTMLIAGHETTAAVLTWTFYLLSKEPSVMLKLQNEVDSVLGDRIPTIEDMKKLKYTTRVINESLRLYPQPPVLIRRSLEDDKLGEYPIKRNEDIFISIWNLHRCPQRWEDADKFNPERWPLDGPNPNETNQAFSYLPFGGGPRKCVGDMFASFEAIVAVAMLVRRFNFQMALGAPPVKMTTGATIHTTEGLNMTVTKRITPPAV-TAETSSLKDDSSVNMSR 592 MA T+ L F + Q+KI NR PT K G++ S + CS SNG+ P+S + + E+ +++ RAELSARIASGEFTVEK+G+ SQL +GL+KLG P E+LD L W A + PKIPEAKGAI AI+SEAFFIPLYELYLTYGG++RLTFGPKSFLIVSDP++AKHILRDNSKAYSKGILAEILEFVMGKGLIPADGEIWRVRRRAIVPALHQKYV AMI++FG+AT+RLC KLDAAASD EDVEMESLFSRLTLDIIGKA+FNYDFDSLTNDTGIVEAVYTVLREAEDRSV+PIP WEIPIWKDISP+ +KV +AL+LIN TLDDLIAICKRMVDEEELQFHEEYMNE DP+IL VSSKQLRDDLMTMLIAGHET+AAVLTWTFYLLS EPSVM KLQ E DSVLGDR PTIED+KKLKY TRVINESLRLYPQPPVLIRRSLEDD+LG+Y IKRNEDIFI IWNLHR P++W+DADKF PERWPLDGPNPNETNQ F YLPFGGGPRKCVGDMFA++E +VA+AMLVRRFNFQMALGAP VKMTTGATIHTTEGL MTVT+RI PP V T E + + D+SV S+ Sbjct: 1 MAATIPLLQFVPTKTFQAKIHSNRLRPT----KQRGTFGLSMVTCSSSNGREPDSVDGVKSVERLLEEKRRAELSARIASGEFTVEKTGYPSQLKNGLSKLGVPREILDFLFSWADAHDGSPKIPEAKGAIGAIQSEAFFIPLYELYLTYGGIYRLTFGPKSFLIVSDPSIAKHILRDNSKAYSKGILAEILEFVMGKGLIPADGEIWRVRRRAIVPALHQKYVAAMINLFGEATERLCQKLDAAASDGEDVEMESLFSRLTLDIIGKALFNYDFDSLTNDTGIVEAVYTVLREAEDRSVAPIPVWEIPIWKDISPRQRKVATALKLINTTLDDLIAICKRMVDEEELQFHEEYMNEQDPSILHFLLASGDDVSSKQLRDDLMTMLIAGHETSAAVLTWTFYLLSTEPSVMSKLQEEADSVLGDRCPTIEDVKKLKYATRVINESLRLYPQPPVLIRRSLEDDRLGDYSIKRNEDIFICIWNLHRSPKQWDDADKFKPERWPLDGPNPNETNQNFRYLPFGGGPRKCVGDMFATYETVVALAMLVRRFNFQMALGAPEVKMTTGATIHTTEGLKMTVTRRIKPPIVPTLEMPTFEVDTSVGASK 600
Match: A0A5E4ECA4 ((Full=PREDICTED: cytochrome {ECO:0000313|EMBL:VVA13076.1}))
HSP 1 Score: 948.347 bits (2450), Expect = 0.000e+0
Identity = 466/613 (76.02%), Postives = 522/613 (85.15%), Query Frame = 0
Query: 1 MATTLNLHLFPAQQFLQSKIQHNRRNPTSTFTKLNGSYRFSGIKCSVSNGKLPNSTEEDEEEEK--KKRIRAELSARIASGEFTVEKSGFQSQLLDGLAKLGAPSEVLDVLSKWIGASEDYPKIPEAKGAISAIRSEAFFIPLYELYLTYGGVFRLTFGPKSFLIVSDPTVAKHILRDNSKAYSKGILAEILEFVMGKGLIPADGEIWRVRRRAIVPALHQKYVTAMISMFGQATDRLCAKLDAAASDAEDVEMESLFSRLTLDIIGKAVFNYDFDSLTNDTGIVEAVYTVLREAEDRSVSPIPFWEIPIWKDISPKLKKVNSALELINGTLDDLIAICKRMVDEEELQFHEEYMNETDPTIL---------VSSKQLRDDLMTMLIAGHETTAAVLTWTFYLLSKEPSVMLKLQNEVDSVLGDRIPTIEDMKKLKYTTRVINESLRLYPQPPVLIRRSLEDDKLGEYPIKRNEDIFISIWNLHRCPQRWEDADKFNPERWPLDGPNPNETNQAFSYLPFGGGPRKCVGDMFASFEAIVAVAMLVRRFNFQMALGAPPVKMTTGATIHTTEGLNMTVTKRITPPAV-TAETSSLKDDSSVNMSR-EAVADQKG 600 MA T+ L F + Q+KI NR PT K G++ S + CS SNG+ P+S + + E+ +++ RAELSARIASGEFTVEK+G+ SQL +GL+KLG P E+LD L W A + PKIPEAKGAI AI+SEAFFIPLYELYLTYGG++RLTFGPKSFLIVSDP++AKHILRDNSKAYSKGILAEILEFVMGKGLIPADGEIWRVRRRAIVPALHQKYV AMI++FG+AT+RLC KLDAAASD EDVEMESLFSRLTLDIIGKA+FNYDFDSLTNDTGIVEAVYTVLREAEDRSV+PIP WEIPIWKDISP+ +KV +AL+LIN TLDDLIAICKRMVDEEELQFHEEYMNE DP+IL VSSKQLRDDLMTMLIAGHET+AAVLTWTFYLLS EPSVM KLQ E DSVLGDR PTIED+KKLKY TRVINESLRLYPQPPVLIRRSLEDD+LG+Y IKRNEDIFI IWNLHR P++W+DADKF PERWPLDGPNPNETNQ F YLPFGGGPRKCVGDMFA++E +VA+AMLVRRFNFQMALGAP VKMTTGATIHTTEGL MTVT+RI PP V T E + + D+SV S+ +++ +KG Sbjct: 1 MAATIPLLQFVPTKTFQAKIHSNRLRPT----KQRGTFGLSMVTCSSSNGREPDSVDGVKSVERLLEEKRRAELSARIASGEFTVEKTGYPSQLKNGLSKLGVPREILDFLFSWADAHDGSPKIPEAKGAIGAIQSEAFFIPLYELYLTYGGIYRLTFGPKSFLIVSDPSIAKHILRDNSKAYSKGILAEILEFVMGKGLIPADGEIWRVRRRAIVPALHQKYVAAMINLFGEATERLCQKLDAAASDGEDVEMESLFSRLTLDIIGKALFNYDFDSLTNDTGIVEAVYTVLREAEDRSVAPIPVWEIPIWKDISPRQRKVATALKLINTTLDDLIAICKRMVDEEELQFHEEYMNEQDPSILHFLLASGDDVSSKQLRDDLMTMLIAGHETSAAVLTWTFYLLSTEPSVMSKLQEEADSVLGDRCPTIEDVKKLKYATRVINESLRLYPQPPVLIRRSLEDDRLGDYSIKRNEDIFICIWNLHRSPKQWDDADKFKPERWPLDGPNPNETNQNFRYLPFGGGPRKCVGDMFATYETVVALAMLVRRFNFQMALGAPEVKMTTGATIHTTEGLKMTVTRRIKPPIVPTLEMPTFEVDTSVGASKGDSLVGKKG 609
Match Name | Stats | Description |
---|---|---|
A0A059P9X5 | E-Value: 0.000e+0, PID: 98.38 | (Chloroplast cytochrome P450 monooxygenase 97A3 {E... [more] |
A0A5N5HWU8 | E-Value: 0.000e+0, PID: 75.93 | (Protein LUTEIN DEFICIENT 5 {ECO:0000313|EMBL:KAB2... [more] |
A0A2C9VBY7 | E-Value: 0.000e+0, PID: 77.45 | (Uncharacterized protein {ECO:0000313|EMBL:OAY4248... [more] |
A0A1S4CL37 | E-Value: 0.000e+0, PID: 75.81 | (protein LUTEIN DEFICIENT 5, chloroplastic-like {E... [more] |
A0A1U7YTG1 | E-Value: 0.000e+0, PID: 75.65 | (protein LUTEIN DEFICIENT 5, chloroplastic {ECO:00... [more] |
A0A314LDR6 | E-Value: 0.000e+0, PID: 75.48 | (Protein lutein deficient 5, chloroplastic {ECO:00... [more] |
A0A059AXX5 | E-Value: 0.000e+0, PID: 76.52 | (Uncharacterized protein {ECO:0000313|EMBL:KCW5872... [more] |
A0A540KET6 | E-Value: 0.000e+0, PID: 76.37 | (Uncharacterized protein {ECO:0000313|EMBL:TQD7274... [more] |
A0A251P439 | E-Value: 0.000e+0, PID: 76.82 | (Uncharacterized protein {ECO:0000313|EMBL:ONI0513... [more] |
A0A5E4ECA4 | E-Value: 0.000e+0, PID: 76.02 | (Full=PREDICTED: cytochrome {ECO:0000313|EMBL:VVA1... [more] |
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An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions. | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |