MATYATWAKPKILTSCPQREVKSQKAYRKNNVNCSLQSPSILHFPKQSPH
IYQTPCVPTTIAQPKHQDSSSSNQWNFLQKAASMALDAVENVIAAREKEH
PLPKTADPQVQIAGNFAPVPEQPVRHRLPVTGKIPECISGVYVRNGANPH
LEPTAGHHFFDGDGMVHAVQFKEGAASYACRFTETERLIQERNLGKPVFP
KAIGELHGHSGIARLALFYARGLFGLVDHSQGTGVANAGLVYFNNRLLAM
SEDDLPYHVEITSSGDLKTVGRYNFDGQLDTTMIAHPKIDPDTKELIALS
YNVIQKPYLKYFRVSPDGVKSDDVDIELADPTMMHDFAITENFVVIPDQQ
VVFKISEMIKGGSPVVYDKNKVSRFGVLDKYAKDGEGIKWVEVPDCFCFH
LWNAWEDEESDEIVVIGSCMTPADSIFNECDEGLQSVLSEIRLNLKTGKS
TRKPIMSSEDQVNLEAGMVNKNKLGRKTQFAYLAIAEPWPKVSGFAKVDL
FTGQVQKFFYGDHKYGGEPLFLPKNPNSENEDDGYILAFVHDEKAWKSEL
QIVNAMTLELEATVQLPSRVPYGFHGTFISAKDLESQA*
Relationships |
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The polypeptide, DCAR_017563, derives from mRNA, DCAR_017563. |
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
Match: Q2VEW9 ((Putative 9-cis epoxycarotenoid dioxygenase {ECO:0000313|EMBL:ABB52079.1}))
HSP 1 Score: 1227.62 bits (3175), Expect = 0.000e+0
Identity = 586/588 (99.66%), Postives = 587/588 (99.83%), Query Frame = 0
Query: 1 MATYATWAKPKILTSCPQREVKSQKAYRKNNVNCSLQSPSILHFPKQSPHIYQTPCVPTTIAQPKHQDSSSSNQWNFLQKAASMALDAVENVIAAREKEHPLPKTADPQVQIAGNFAPVPEQPVRHRLPVTGKIPECISGVYVRNGANPHLEPTAGHHFFDGDGMVHAVQFKEGAASYACRFTETERLIQERNLGKPVFPKAIGELHGHSGIARLALFYARGLFGLVDHSQGTGVANAGLVYFNNRLLAMSEDDLPYHVEITSSGDLKTVGRYNFDGQLDTTMIAHPKIDPDTKELIALSYNVIQKPYLKYFRVSPDGVKSDDVDIELADPTMMHDFAITENFVVIPDQQVVFKISEMIKGGSPVVYDKNKVSRFGVLDKYAKDGEGIKWVEVPDCFCFHLWNAWEDEESDEIVVIGSCMTPADSIFNECDEGLQSVLSEIRLNLKTGKSTRKPIMSSEDQVNLEAGMVNKNKLGRKTQFAYLAIAEPWPKVSGFAKVDLFTGQVQKFFYGDHKYGGEPLFLPKNPNSENEDDGYILAFVHDEKAWKSELQIVNAMTLELEATVQLPSRVPYGFHGTFISAKDLESQA 588 MATYATWAKPKILTSCPQREVKSQKAYRKNNVNCSLQSPSILHFPKQSPHIYQTPCVPTTIAQPKHQDSSSSNQWNFLQKAASMALDAVENVIAAREKEHPLPKTADPQVQIAGNFAPVPEQPVRHRLPVTGKIPECISGVYVRNGANPHLEPTAGHHFFDGDGMVHAVQFKEGAASYACRFTETERLIQERNLGKPVFPKAIGELHGHSGIARLALFYARGLFGLVDHSQGTGVANAGLVYFNNRLLAMSEDDLPYHVEITSSGDLKTVGRYNFDGQLDTTMIAHPKIDPDTKELIALSYNVIQKPYLKYFRVSPDGVKSDDVDIELADPTMMHDFAITENFVVIPDQQVVFKISEMI+GGSPVVYDKNKVSRFGVLDKYAKDG GIKWVEVPDCFCFHLWNAWEDEESDEIVVIGSCMTPADSIFNECDEGLQSVLSEIRLNLKTGKSTRKPIMSSEDQVNLEAGMVNKNKLGRKTQFAYLAIAEPWPKVSGFAKVDLFTGQVQKFFYGDHKYGGEPLFLPKNPNSENEDDGYILAFVHDEKAWKSELQIVNAMTLELEATVQLPSRVPYGFHGTFISAKDLESQA Sbjct: 1 MATYATWAKPKILTSCPQREVKSQKAYRKNNVNCSLQSPSILHFPKQSPHIYQTPCVPTTIAQPKHQDSSSSNQWNFLQKAASMALDAVENVIAAREKEHPLPKTADPQVQIAGNFAPVPEQPVRHRLPVTGKIPECISGVYVRNGANPHLEPTAGHHFFDGDGMVHAVQFKEGAASYACRFTETERLIQERNLGKPVFPKAIGELHGHSGIARLALFYARGLFGLVDHSQGTGVANAGLVYFNNRLLAMSEDDLPYHVEITSSGDLKTVGRYNFDGQLDTTMIAHPKIDPDTKELIALSYNVIQKPYLKYFRVSPDGVKSDDVDIELADPTMMHDFAITENFVVIPDQQVVFKISEMIQGGSPVVYDKNKVSRFGVLDKYAKDGSGIKWVEVPDCFCFHLWNAWEDEESDEIVVIGSCMTPADSIFNECDEGLQSVLSEIRLNLKTGKSTRKPIMSSEDQVNLEAGMVNKNKLGRKTQFAYLAIAEPWPKVSGFAKVDLFTGQVQKFFYGDHKYGGEPLFLPKNPNSENEDDGYILAFVHDEKAWKSELQIVNAMTLELEATVQLPSRVPYGFHGTFISAKDLESQA 588
Match: Q2VEW8 ((Putative 9-cis epoxycarotenoid dioxygenase {ECO:0000313|EMBL:ABB52080.1}))
HSP 1 Score: 1067.76 bits (2760), Expect = 0.000e+0
Identity = 511/587 (87.05%), Postives = 546/587 (93.02%), Query Frame = 0
Query: 5 ATWAKPKILTSCPQREVKSQKAYRKNNVNCSLQS-PSILHFPKQSPHIYQTPCVPTTIAQPKHQDSSSSN--QWNFLQKAASMALDAVENVIAAREKEHPLPKTADPQVQIAGNFAPVPEQPVRHRLPVTGKIPECISGVYVRNGANPHLEPTAGHHFFDGDGMVHAVQFKEGAASYACRFTETERLIQERNLGKPVFPKAIGELHGHSGIARLALFYARGLFGLVDHSQGTGVANAGLVYFNNRLLAMSEDDLPYHVEITSSGDLKTVGRYNFDGQLDTTMIAHPKIDPDTKELIALSYNVIQKPYLKYFRVSPDGVKSDDVDIELADPTMMHDFAITENFVVIPDQQVVFKISEMIKGGSPVVYDKNKVSRFGVLDKYAKDGEGIKWVEVPDCFCFHLWNAWEDEESDEIVVIGSCMTPADSIFNECDEGLQSVLSEIRLNLKTGKSTRKPIMSSEDQVNLEAGMVNKNKLGRKTQFAYLAIAEPWPKVSGFAKVDLFTGQVQKFFYGDHKYGGEPLFLPKNPNSENEDDGYILAFVHDEKAWKSELQIVNAMTLELEATVQLPSRVPYGFHGTFISAKDLESQA 588 +TWAKP I +S QR+ + RK NVNC++QS PSILHFPKQS YQTP +PTTI QPKHQDSSSS +WNFLQKAASMALDAVEN+I ++E+ HPLPKTADPQVQI+GNFAPV EQPV+H LPVTGKIPECISGVYVRNGANPH EPT GHHFFDGDGMVHAV+FK+G ASYACR+TETERLIQERNLG+ VFPKAIGELHGHSGIARLALFYARGLFGLVDHSQGTGVANAGLVYFNNRLLAMSEDDLPYHV+IT GDL+TV RY+FDGQLD+TMIAHPKIDP+T ELIAL+YNVIQKPYLKYFRVSPDGVKS DVDIELADPTMMHDFAITENFVVIPDQQVVFKISEMI+GGSPVVYDK KVSRFGVLDKYAKDG GIKWVEVPDCFCFHLWNAWED+ESDEIVVIGSCMTPADSIFNECDEGL+SVLSEIRLNLKTGKSTR+ I+S EDQVNLEAGMVN NKLGRKTQFAYLAIAEPWPKVSGFAKVDLF G+++KFFYGD+KYGGEPLFLP+NPNSENEDDGYILAFVHDEKAWKSELQIVNAMTLELEATV+LPSRVPYGFHGTFIS KDLE QA Sbjct: 9 STWAKPNIYSS--QRD-----SPRKTNVNCAIQSSPSILHFPKQSKQKYQTPSIPTTIVQPKHQDSSSSATPKWNFLQKAASMALDAVENIIISQEQSHPLPKTADPQVQISGNFAPVQEQPVQHHLPVTGKIPECISGVYVRNGANPHFEPTGGHHFFDGDGMVHAVKFKDGEASYACRYTETERLIQERNLGRTVFPKAIGELHGHSGIARLALFYARGLFGLVDHSQGTGVANAGLVYFNNRLLAMSEDDLPYHVQITPKGDLRTVERYSFDGQLDSTMIAHPKIDPETNELIALTYNVIQKPYLKYFRVSPDGVKSSDVDIELADPTMMHDFAITENFVVIPDQQVVFKISEMIQGGSPVVYDKEKVSRFGVLDKYAKDGSGIKWVEVPDCFCFHLWNAWEDKESDEIVVIGSCMTPADSIFNECDEGLESVLSEIRLNLKTGKSTRRAIISPEDQVNLEAGMVNINKLGRKTQFAYLAIAEPWPKVSGFAKVDLFNGEIKKFFYGDNKYGGEPLFLPRNPNSENEDDGYILAFVHDEKAWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISEKDLEKQA 588
Match: A0A1U7Y7N0 ((9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic-like {ECO:0000313|RefSeq:XP_009797946.1}))
HSP 1 Score: 966.452 bits (2497), Expect = 0.000e+0
Identity = 462/601 (76.87%), Postives = 524/601 (87.19%), Query Frame = 0
Query: 6 TWAKPKI-------LTSCPQREVKSQKAYRKNNVNCSLQSPSILHFPKQSPHIYQTPCV-------PTTIAQPKHQDSSSSN---QWNFLQKAASMALDAVENVIAAREKEHPLPKTADPQVQIAGNFAPVPEQPVRHRLPVTGKIPECISGVYVRNGANPHLEPTAGHHFFDGDGMVHAVQFKEGAASYACRFTETERLIQERNLGKPVFPKAIGELHGHSGIARLALFYARGLFGLVDHSQGTGVANAGLVYFNNRLLAMSEDDLPYHVEITSSGDLKTVGRYNFDGQLDTTMIAHPKIDPDTKELIALSYNVIQKPYLKYFRVSPDGVKSDDVDIELADPTMMHDFAITENFVVIPDQQVVFKISEMIKGGSPVVYDKNKVSRFGVLDKYAKDGEGIKWVEVPDCFCFHLWNAWEDEESDEIVVIGSCMTPADSIFNECDEGLQSVLSEIRLNLKTGKSTRKPIMSS-EDQVNLEAGMVNKNKLGRKTQFAYLAIAEPWPKVSGFAKVDLFTGQVQKFFYGDHKYGGEPLFLPKNPNSENEDDGYILAFVHDEKAWKSELQIVNAMTLELEATVQLPSRVPYGFHGTFISAKDLESQA 588 TW KPKI +S V Q + ++N SLQ+P ILHFPKQSP+ YQTP PTTI+ PK +++SSS QWN +QKAA+MALDAVE+ + E EHPLPKTADP+VQI+GNFAPVPE PV H LPVTGKIP+C+ GVYVRNGANP EPTAGHHFFDGDGMVHAVQFK G+ASYACRFTETERL+QE+ LG+PVFPKAIGELHGHSGIARL LFYARGLFGLVDHS+GTGVANAGLVYFNNRLLAMSEDDLPYHV +T +GDLKTVGR++FDGQL +TMIAHPK+DP + EL ALSY+VIQKPYLKYFR S +G KS+DV+I + DPTMMHDFAIT+ +V+IPDQQVVFK+SEMI+GGSPVVYDKNKVSRFGVLDKYAKDGEG+KWVEVPDCFCFHLWNAWE+ E+DEIVVIGSCMTP DSIFNECDEGL+SVLSEIRLNLKTGKSTR+ I+ EDQVNLEAGMVN+NKLGRKT+FAYLAIAEPWPKVSGFAKVDLFTG+V+KF YGD+K+GGEPLFLP++PNS+ EDDGYILAF H+EK WKSELQIVNAMT++LEATV+LP+RVPYGFHGTFI+AKDL +QA Sbjct: 10 TWIKPKISMPRDLAYSSNSISLVNKQPNRQLPSINSSLQAPPILHFPKQSPN-YQTPKTSTISTSKPTTISYPKQENNSSSTTHPQWNLVQKAAAMALDAVESALTKHELEHPLPKTADPRVQISGNFAPVPENPVSHSLPVTGKIPKCVQGVYVRNGANPLFEPTAGHHFFDGDGMVHAVQFKNGSASYACRFTETERLVQEKALGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSKGTGVANAGLVYFNNRLLAMSEDDLPYHVRVTPTGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGELFALSYDVIQKPYLKYFRFSKNGEKSNDVEIPVEDPTMMHDFAITDKYVIIPDQQVVFKMSEMIRGGSPVVYDKNKVSRFGVLDKYAKDGEGLKWVEVPDCFCFHLWNAWEEPETDEIVVIGSCMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRRAIIQKEEDQVNLEAGMVNRNKLGRKTRFAYLAIAEPWPKVSGFAKVDLFTGEVEKFIYGDNKFGGEPLFLPRDPNSKAEDDGYILAFAHNEKEWKSELQIVNAMTMKLEATVKLPTRVPYGFHGTFINAKDLANQA 609
Match: A0A0N7CTG4 ((9-cis-epoxycarotenoid dioxygenase 3-2 {ECO:0000313|EMBL:AKO22634.1}) (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic-like {ECO:0000313|RefSeq:XP_016510306.1}))
HSP 1 Score: 966.452 bits (2497), Expect = 0.000e+0
Identity = 462/601 (76.87%), Postives = 524/601 (87.19%), Query Frame = 0
Query: 6 TWAKPKI-------LTSCPQREVKSQKAYRKNNVNCSLQSPSILHFPKQSPHIYQTPCV-------PTTIAQPKHQDSSSSN---QWNFLQKAASMALDAVENVIAAREKEHPLPKTADPQVQIAGNFAPVPEQPVRHRLPVTGKIPECISGVYVRNGANPHLEPTAGHHFFDGDGMVHAVQFKEGAASYACRFTETERLIQERNLGKPVFPKAIGELHGHSGIARLALFYARGLFGLVDHSQGTGVANAGLVYFNNRLLAMSEDDLPYHVEITSSGDLKTVGRYNFDGQLDTTMIAHPKIDPDTKELIALSYNVIQKPYLKYFRVSPDGVKSDDVDIELADPTMMHDFAITENFVVIPDQQVVFKISEMIKGGSPVVYDKNKVSRFGVLDKYAKDGEGIKWVEVPDCFCFHLWNAWEDEESDEIVVIGSCMTPADSIFNECDEGLQSVLSEIRLNLKTGKSTRKPIMSS-EDQVNLEAGMVNKNKLGRKTQFAYLAIAEPWPKVSGFAKVDLFTGQVQKFFYGDHKYGGEPLFLPKNPNSENEDDGYILAFVHDEKAWKSELQIVNAMTLELEATVQLPSRVPYGFHGTFISAKDLESQA 588 TW KPKI +S V Q + ++N SLQ+P ILHFPKQSP+ YQTP PTTI+ PK +++SSS QWN +QKAA+MALDAVE+ + E EHPLPKTADP+VQI+GNFAPVPE PV H LPVTGKIP+C+ GVYVRNGANP EPTAGHHFFDGDGMVHAVQFK G+ASYACRFTETERL+QE+ LG+PVFPKAIGELHGHSGIARL LFYARGLFGLVDHS+GTGVANAGLVYFNNRLLAMSEDDLPYHV +T +GDLKTVGR++FDGQL +TMIAHPK+DP + EL ALSY+VIQKPYLKYFR S +G KS+DV+I + DPTMMHDFAIT+ +V+IPDQQVVFK+SEMI+GGSPVVYDKNKVSRFGVLDKYAKDGEG+KWVEVPDCFCFHLWNAWE+ E+DEIVVIGSCMTP DSIFNECDEGL+SVLSEIRLNLKTGKSTR+ I+ EDQVNLEAGMVN+NKLGRKT+FAYLAIAEPWPKVSGFAKVDLFTG+V+KF YGD+K+GGEPLFLP++PNS+ EDDGYILAF H+EK WKSELQIVNAMT++LEATV+LP+RVPYGFHGTFI+AKDL +QA Sbjct: 10 TWIKPKISMPRDLAYSSNSISLVNKQPNRQLPSINSSLQAPPILHFPKQSPN-YQTPKTSTISTSKPTTISYPKQENNSSSTTHPQWNLVQKAAAMALDAVESALTKHELEHPLPKTADPRVQISGNFAPVPENPVSHSLPVTGKIPKCVQGVYVRNGANPLFEPTAGHHFFDGDGMVHAVQFKNGSASYACRFTETERLVQEKALGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSKGTGVANAGLVYFNNRLLAMSEDDLPYHVRVTPTGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGELFALSYDVIQKPYLKYFRFSKNGEKSNDVEIPVEDPTMMHDFAITDKYVIIPDQQVVFKMSEMIRGGSPVVYDKNKVSRFGVLDKYAKDGEGLKWVEVPDCFCFHLWNAWEEPETDEIVVIGSCMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRRAIIQKEEDQVNLEAGMVNRNKLGRKTRFAYLAIAEPWPKVSGFAKVDLFTGEVEKFIYGDNKFGGEPLFLPRDPNSKAEDDGYILAFAHNEKEWKSELQIVNAMTMKLEATVKLPTRVPYGFHGTFINAKDLANQA 609
Match: A0A0A7DW45 ((9-cis-epoxycarotenoid dioxygenase 1 {ECO:0000313|EMBL:AIX87507.1}))
HSP 1 Score: 964.911 bits (2493), Expect = 0.000e+0
Identity = 460/598 (76.92%), Postives = 523/598 (87.46%), Query Frame = 0
Query: 6 TWAKPKILTSCPQREVKSQKAY-----RKNNVNCSLQSPSILHFPKQSPHIYQTP---CVPTT-----IAQPKHQD-SSSSNQWNFLQKAASMALDAVENVIAAREKEHPLPKTADPQVQIAGNFAPVPEQPVRHRLPVTGKIPECISGVYVRNGANPHLEPTAGHHFFDGDGMVHAVQFKEGAASYACRFTETERLIQERNLGKPVFPKAIGELHGHSGIARLALFYARGLFGLVDHSQGTGVANAGLVYFNNRLLAMSEDDLPYHVEITSSGDLKTVGRYNFDGQLDTTMIAHPKIDPDTKELIALSYNVIQKPYLKYFRVSPDGVKSDDVDIELADPTMMHDFAITENFVVIPDQQVVFKISEMIKGGSPVVYDKNKVSRFGVLDKYAKDGEGIKWVEVPDCFCFHLWNAWEDEESDEIVVIGSCMTPADSIFNECDEGLQSVLSEIRLNLKTGKSTRKPIM-SSEDQVNLEAGMVNKNKLGRKTQFAYLAIAEPWPKVSGFAKVDLFTGQVQKFFYGDHKYGGEPLFLPKNPNSENEDDGYILAFVHDEKAWKSELQIVNAMTLELEATVQLPSRVPYGFHGTFISAKDLESQA 588 TW KPKI + S + + N + CSLQ+P ILHFPKQ YQTP +PT+ I+ PK ++ SSSS+ WN +QKAA+MALDAVE+V+ E EHPLPKTADP+VQI+GNFAPVPE PV LPVTGKIP+C++GVYVRNGANP EPTAGHHFFDGDGMVHAV+FK G+ASYACRFTETERL+QE+ LG+PVFPKAIGELHGHSGIARL LFYARG+FGLVDHS GTGVANAGLVYFNNRLLAMSEDDLPYHV +T++GDL+T GR++FDGQL +TMIAHPK+DP + EL ALSY+VIQKPYLKYFR S +G KS+DV+I + DPTMMHDFAITE FVVIPDQQVVFK+SEMI+GGSPVVYDKNKVSRFG+LDKYAKDG G+KWVEVPDCFCFHLWNAWE+ E+DEIVVIGSCMTP DSIFNECDEGL+SVLSEIRLNLKTGKSTRK I+ + E+QVNLEAGMVN+NKLGRKTQ+AYLAIAEPWPKVSGFAKVDLFTG++ KFFYGD+KYGGEPLFLP++PNS++EDDGYILAFVHDEK WKSELQIVNAMTL+LEATV+LPSRVPYGFHGTFI+AKDL +QA Sbjct: 10 TWIKPKISMPSSREFGHSSNSISLLKNKPNKITCSLQAPPILHFPKQQSSNYQTPKTNTIPTSKPTTKISHPKQENKSSSSSPWNLVQKAAAMALDAVESVLTKHELEHPLPKTADPRVQISGNFAPVPENPVTQSLPVTGKIPKCVNGVYVRNGANPLFEPTAGHHFFDGDGMVHAVEFKNGSASYACRFTETERLVQEKALGRPVFPKAIGELHGHSGIARLMLFYARGVFGLVDHSNGTGVANAGLVYFNNRLLAMSEDDLPYHVRVTANGDLETDGRFDFDGQLKSTMIAHPKLDPVSGELFALSYDVIQKPYLKYFRFSKNGEKSNDVEIPVEDPTMMHDFAITEKFVVIPDQQVVFKMSEMIRGGSPVVYDKNKVSRFGILDKYAKDGSGLKWVEVPDCFCFHLWNAWEEPETDEIVVIGSCMTPPDSIFNECDEGLESVLSEIRLNLKTGKSTRKAIIQNEEEQVNLEAGMVNRNKLGRKTQYAYLAIAEPWPKVSGFAKVDLFTGEINKFFYGDNKYGGEPLFLPRDPNSKDEDDGYILAFVHDEKEWKSELQIVNAMTLKLEATVKLPSRVPYGFHGTFINAKDLANQA 607
Match: A0A0N7CUA2 ((9-cis-epoxycarotenoid dioxygenase 3-1 {ECO:0000313|EMBL:AKO22633.1}) (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic-like {ECO:0000313|RefSeq:XP_016478117.1}))
HSP 1 Score: 963.37 bits (2489), Expect = 0.000e+0
Identity = 464/605 (76.69%), Postives = 527/605 (87.11%), Query Frame = 0
Query: 6 TWAKPKI---------LTSCPQREVKSQKAYRKNNVNCSLQSPSILHFPKQSPHIYQTPCV-------PTTIAQPKHQDSSSSN-----QWNFLQKAASMALDAVENVIAAREKEHPLPKTADPQVQIAGNFAPVPEQPVRHRLPVTGKIPECISGVYVRNGANPHLEPTAGHHFFDGDGMVHAVQFKEGAASYACRFTETERLIQERNLGKPVFPKAIGELHGHSGIARLALFYARGLFGLVDHSQGTGVANAGLVYFNNRLLAMSEDDLPYHVEITSSGDLKTVGRYNFDGQLDTTMIAHPKIDPDTKELIALSYNVIQKPYLKYFRVSPDGVKSDDVDIELADPTMMHDFAITENFVVIPDQQVVFKISEMIKGGSPVVYDKNKVSRFGVLDKYAKDGEGIKWVEVPDCFCFHLWNAWEDEESDEIVVIGSCMTPADSIFNECDEGLQSVLSEIRLNLKTGKSTRKPIMSS-EDQVNLEAGMVNKNKLGRKTQFAYLAIAEPWPKVSGFAKVDLFTGQVQKFFYGDHKYGGEPLFLPKNPNSENEDDGYILAFVHDEKAWKSELQIVNAMTLELEATVQLPSRVPYGFHGTFISAKDLESQA 588 TW KPK+ +S VK Q + ++N SLQ+P ILHFPKQSP+ YQTP PTTI+ PK + ++SS+ QWN +QKAA+MALDAVE+V+ E EHPLPKTADP+VQI+GNFAPVPE PV H LPVTGKIP+C+ GVYVRNGANP EPTAGHHFFDGDGMVHAVQFK G+ASYACRFTETERL+QE+ LG+PVFPKAIGELHGHSGIARL LFYARGLFGLVDHS+GTGVANAGLVYFNNRLLAMSEDDLPYHV +T +GDLKTVGR++FDGQL +TMIAHPK+DP + EL ALSY+VIQKPYLKYFR S +G KS+DV+I + DPTMMHDFAITE FV+IPDQQVVFK+SEMI+GGSPVVYDKNKVSRFGVLDKYAKDGEG+KWVEVPDCFCFHLWNAWE+ E+DEIVVIGSCMTP DSIFNECDEGL+SVLSEIRLNLKTGKSTR+ I+ EDQVNLEAGMVN+NKLGRKT+FAYLAIAEPWPKVSGFAKVDLFTG+V+KF YGD+K+GGEPLFLP++PNS+ EDDGYILAFVH+EK WKSELQIVNAMT++LEATV+LP+RVPYGFHGTFI+AKD+ +QA Sbjct: 10 TWIKPKLSMPSSRDLAYSSNSISLVKKQPNRQLPSINSSLQAPPILHFPKQSPN-YQTPKTTTIPTSKPTTISYPKQESTNSSSSTTSPQWNLVQKAAAMALDAVESVLTKHELEHPLPKTADPRVQISGNFAPVPENPVSHSLPVTGKIPKCVQGVYVRNGANPLFEPTAGHHFFDGDGMVHAVQFKNGSASYACRFTETERLVQEKALGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSRGTGVANAGLVYFNNRLLAMSEDDLPYHVRVTPTGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGELFALSYDVIQKPYLKYFRFSKNGEKSNDVEIPVEDPTMMHDFAITEKFVIIPDQQVVFKMSEMIRGGSPVVYDKNKVSRFGVLDKYAKDGEGLKWVEVPDCFCFHLWNAWEEPETDEIVVIGSCMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRRAIIQKEEDQVNLEAGMVNRNKLGRKTRFAYLAIAEPWPKVSGFAKVDLFTGEVEKFIYGDNKFGGEPLFLPRDPNSKAEDDGYILAFVHNEKEWKSELQIVNAMTMKLEATVKLPTRVPYGFHGTFINAKDMANQA 613
Match: A0A2G2WEP9 ((9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic {ECO:0000313|EMBL:PHT43711.1}))
HSP 1 Score: 962.214 bits (2486), Expect = 0.000e+0
Identity = 463/605 (76.53%), Postives = 524/605 (86.61%), Query Frame = 0
Query: 1 MATYATWAKPKILTSCPQREVKSQKAYR--KN-----NVNCSLQSPSILHFPKQSPHIYQTPCV-------PTTIAQPKHQDSS--SSNQWNFLQKAASMALDAVENVIAAREKEHPLPKTADPQVQIAGNFAPVPEQPVRHRLPVTGKIPECISGVYVRNGANPHLEPTAGHHFFDGDGMVHAVQFKEGAASYACRFTETERLIQERNLGKPVFPKAIGELHGHSGIARLALFYARGLFGLVDHSQGTGVANAGLVYFNNRLLAMSEDDLPYHVEITSSGDLKTVGRYNFDGQLDTTMIAHPKIDPDTKELIALSYNVIQKPYLKYFRVSPDGVKSDDVDIELADPTMMHDFAITENFVVIPDQQVVFKISEMIKGGSPVVYDKNKVSRFGVLDKYAKDGEGIKWVEVPDCFCFHLWNAWEDEESDEIVVIGSCMTPADSIFNECDEGLQSVLSEIRLNLKTGKSTRKPIMSS-EDQVNLEAGMVNKNKLGRKTQFAYLAIAEPWPKVSGFAKVDLFTGQVQKFFYGDHKYGGEPLFLPKNPNSENEDDGYILAFVHDEKAWKSELQIVNAMTLELEATVQLPSRVPYGFHGTFISAKDLESQA 588 MAT TW KPK+ + V S ++ KN N+NCSLQ+P +LHFPKQS YQTP PTT +QPK ++++ S+ +WN +QKAA+MALDAVE+ + E EHPLPKTADP+VQI+GNFAPVPE+PV L VTGKIP+C+ GVYVRNGANP EPTAGHHFFDGDGMVHAVQFK+G+ASYACRFTETER +QE+ LG+PVFPKAIGELHGHSGIARL LFYARGLFG+VDHS+GTGVANAGLVYFNNRLLAMSEDDLPYHV++T +GDLKT GR++F+GQL +TMIAHPK+DP + EL ALSY+VIQKPYLKYFR S DG KSDDV+I + DPTMMHDFAITE FVVIPDQQVVFK+SEMI+GGSPVVYDK KVSRFGVLDKYAKDG G+KW+EVPDCFCFHLWNAWEDE+ DEIVVIGSCMTP DSIFNECDEGL+SVLSEIRLNLKTGKSTRK I+ + E+QVNLEAGMVN+NKLGRKTQFAYLAIAEPWPKVSGFAKVDL TG+V KF YGD+KYGGEPLFLP++ NS+ EDDGYILAFVHDEK WKSELQIVNAMTL+LEATV+LPSRVPYGFHGTFI+AKDL +QA Sbjct: 1 MAT-NTWIKPKLSMPSSKEFVFSSNSFPLIKNQRQIPNINCSLQAPPLLHFPKQSTTTYQTPKTNTISTPKPTTTSQPKQENNTNTSTCKWNLVQKAAAMALDAVESALTKHELEHPLPKTADPRVQISGNFAPVPEKPVTQSLQVTGKIPKCVQGVYVRNGANPLFEPTAGHHFFDGDGMVHAVQFKDGSASYACRFTETERFVQEKALGRPVFPKAIGELHGHSGIARLMLFYARGLFGIVDHSKGTGVANAGLVYFNNRLLAMSEDDLPYHVKVTPTGDLKTEGRFDFNGQLKSTMIAHPKLDPVSGELFALSYDVIQKPYLKYFRFSKDGEKSDDVEIPVEDPTMMHDFAITEKFVVIPDQQVVFKMSEMIRGGSPVVYDKEKVSRFGVLDKYAKDGSGLKWIEVPDCFCFHLWNAWEDEDCDEIVVIGSCMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRKTIIQNEEEQVNLEAGMVNRNKLGRKTQFAYLAIAEPWPKVSGFAKVDLLTGEVHKFIYGDNKYGGEPLFLPRDSNSQAEDDGYILAFVHDEKEWKSELQIVNAMTLKLEATVKLPSRVPYGFHGTFINAKDLANQA 604
Match: A0A0A7EBR7 ((9-cis epoxycarotenoid dioxygenase {ECO:0000313|EMBL:AIY62759.1}))
HSP 1 Score: 961.059 bits (2483), Expect = 0.000e+0
Identity = 459/598 (76.76%), Postives = 521/598 (87.12%), Query Frame = 0
Query: 6 TWAKPKILTSCPQREVKSQKAY-----RKNNVNCSLQSPSILHFPKQSPHIYQTP---CVPTT-----IAQPKHQD-SSSSNQWNFLQKAASMALDAVENVIAAREKEHPLPKTADPQVQIAGNFAPVPEQPVRHRLPVTGKIPECISGVYVRNGANPHLEPTAGHHFFDGDGMVHAVQFKEGAASYACRFTETERLIQERNLGKPVFPKAIGELHGHSGIARLALFYARGLFGLVDHSQGTGVANAGLVYFNNRLLAMSEDDLPYHVEITSSGDLKTVGRYNFDGQLDTTMIAHPKIDPDTKELIALSYNVIQKPYLKYFRVSPDGVKSDDVDIELADPTMMHDFAITENFVVIPDQQVVFKISEMIKGGSPVVYDKNKVSRFGVLDKYAKDGEGIKWVEVPDCFCFHLWNAWEDEESDEIVVIGSCMTPADSIFNECDEGLQSVLSEIRLNLKTGKSTRKP-IMSSEDQVNLEAGMVNKNKLGRKTQFAYLAIAEPWPKVSGFAKVDLFTGQVQKFFYGDHKYGGEPLFLPKNPNSENEDDGYILAFVHDEKAWKSELQIVNAMTLELEATVQLPSRVPYGFHGTFISAKDLESQA 588 TW KPKI + S + + N + CSLQ+P ILHFPKQ YQTP +PT+ I+ PK ++ SSSS+ N +QKAA+MALDAVE+V+ E EHPLPKTADP+VQI+GNFAPVPE PV LPVTGKIP+C++GVYVRNGANP EPTAGHHFFDGDGMVHAVQFK G+ASYACRFTETERL+QE+ LG+PVFPKAIGELHGHSGIARL LFYARG+FGLVDHS GTGVANAGLVYFNNRLLAMSEDDLPYHV +T++GDL+T GR++FDGQL +TMIAHPK+DP + EL ALSY+VIQKPYLKYFR S +G KS+DV+I + DPTMMHDFAITE FVVIPDQQVVFK+SEMI+GGSPVVYDKNKVSRFG+LDKYAKDG G+KWVEVPDCFCFHLWNAWE+ E+DE+VVIGSCMTP DSIFNECDEGL+SVLSEIRLNLKTGKSTRK I + E+QVNLEAGMVN+NKLGRKTQ+AYLAIAEPWPKVSGFAKVDLFTG++ KFFYGD+KYGGEPLFLP++PNS++EDDGYILAFVHDEK WKSELQIVNAMTL+LEATV+LPSRVPYGFHGTFI+AKDL +QA Sbjct: 11 TWIKPKISMPSSREFGHSSNSISLLKNKPNKITCSLQAPPILHFPKQQSSNYQTPKTNTIPTSKPTTKISHPKQENKSSSSSPLNLVQKAAAMALDAVESVLTKHELEHPLPKTADPRVQISGNFAPVPENPVTQSLPVTGKIPKCVNGVYVRNGANPLFEPTAGHHFFDGDGMVHAVQFKNGSASYACRFTETERLVQEKALGRPVFPKAIGELHGHSGIARLMLFYARGVFGLVDHSNGTGVANAGLVYFNNRLLAMSEDDLPYHVRVTANGDLETDGRFDFDGQLKSTMIAHPKLDPVSGELFALSYDVIQKPYLKYFRFSKNGEKSNDVEIPVEDPTMMHDFAITEKFVVIPDQQVVFKMSEMIRGGSPVVYDKNKVSRFGILDKYAKDGSGLKWVEVPDCFCFHLWNAWEEPETDELVVIGSCMTPPDSIFNECDEGLESVLSEIRLNLKTGKSTRKALIQNEEEQVNLEAGMVNRNKLGRKTQYAYLAIAEPWPKVSGFAKVDLFTGEINKFFYGDNKYGGEPLFLPRDPNSKDEDDGYILAFVHDEKEWKSELQIVNAMTLKLEATVKLPSRVPYGFHGTFINAKDLANQA 608
Match: W5QL26 ((9-cis-epoxy-carotenoid dioxygenase {ECO:0000313|EMBL:AFO85470.1}))
HSP 1 Score: 960.674 bits (2482), Expect = 0.000e+0
Identity = 459/598 (76.76%), Postives = 521/598 (87.12%), Query Frame = 0
Query: 6 TWAKPKILTSCPQREVKSQKAY-----RKNNVNCSLQSPSILHFPKQSPHIYQTP---CVPTT-----IAQPKHQD-SSSSNQWNFLQKAASMALDAVENVIAAREKEHPLPKTADPQVQIAGNFAPVPEQPVRHRLPVTGKIPECISGVYVRNGANPHLEPTAGHHFFDGDGMVHAVQFKEGAASYACRFTETERLIQERNLGKPVFPKAIGELHGHSGIARLALFYARGLFGLVDHSQGTGVANAGLVYFNNRLLAMSEDDLPYHVEITSSGDLKTVGRYNFDGQLDTTMIAHPKIDPDTKELIALSYNVIQKPYLKYFRVSPDGVKSDDVDIELADPTMMHDFAITENFVVIPDQQVVFKISEMIKGGSPVVYDKNKVSRFGVLDKYAKDGEGIKWVEVPDCFCFHLWNAWEDEESDEIVVIGSCMTPADSIFNECDEGLQSVLSEIRLNLKTGKSTRKP-IMSSEDQVNLEAGMVNKNKLGRKTQFAYLAIAEPWPKVSGFAKVDLFTGQVQKFFYGDHKYGGEPLFLPKNPNSENEDDGYILAFVHDEKAWKSELQIVNAMTLELEATVQLPSRVPYGFHGTFISAKDLESQA 588 TW KPKI + S + + N + CSLQ+P ILHFPKQ YQTP +PT+ I+ PK ++ SSSS+ N +QKAA+MALDAVE+V+ E EHPLPKTADP+VQI+GNFAPVPE PV LPVTGKIP+C++GVYVRNGANP EPTAGHHFFDGDGMVHAVQFK G+ASYACRFTETERL+QE+ LG+PVFPKAIGELHGHSGIARL LFYARG+FGLVDHS GTGVANAGLVYFNNRLLAMSEDDLPYHV +T++GDL+T GR++FDGQL +TMIAHPK+DP + EL ALSY+VIQKPYLKYFR S +G KS+DV+I + DPTMMHDFAITE FVVIPDQQVVFK+SEMI+GGSPVVYDKNKVSRFG+LDKYAKDG G+KWVEVPDCFCFHLWNAWE+ E+DE+VVIGSCMTP DSIFNECDEGL+SVLSEIRLNLKTGKSTRK I + E+QVNLEAGMVN+NKLGRKTQ+AYLAIAEPWPKVSGFAKVDLFTG++ KFFYGD+KYGGEPLFLP++PNS++EDDGYILAFVHDEK WKSELQIVNAMTL+LEATV+LPSRVPYGFHGTFI+AKDL +QA Sbjct: 10 TWIKPKISMPSSREFGHSSNSISLLKNKPNKITCSLQAPPILHFPKQQSSNYQTPKTNTIPTSKPTTKISHPKQENKSSSSSPLNLVQKAAAMALDAVESVLTKHELEHPLPKTADPRVQISGNFAPVPENPVTQSLPVTGKIPKCVNGVYVRNGANPLFEPTAGHHFFDGDGMVHAVQFKNGSASYACRFTETERLVQEKALGRPVFPKAIGELHGHSGIARLMLFYARGVFGLVDHSNGTGVANAGLVYFNNRLLAMSEDDLPYHVRVTANGDLETDGRFDFDGQLKSTMIAHPKLDPVSGELFALSYDVIQKPYLKYFRFSKNGEKSNDVEIPVEDPTMMHDFAITEKFVVIPDQQVVFKMSEMIRGGSPVVYDKNKVSRFGILDKYAKDGSGLKWVEVPDCFCFHLWNAWEEPETDELVVIGSCMTPPDSIFNECDEGLESVLSEIRLNLKTGKSTRKALIQNEEEQVNLEAGMVNRNKLGRKTQYAYLAIAEPWPKVSGFAKVDLFTGEINKFFYGDNKYGGEPLFLPRDPNSKDEDDGYILAFVHDEKEWKSELQIVNAMTLKLEATVKLPSRVPYGFHGTFINAKDLANQA 607
Match: A0A0A7DVZ4 ((9-cis-epoxycarotenoid dioxygenase 1 {ECO:0000313|EMBL:AIX87531.1}))
HSP 1 Score: 959.518 bits (2479), Expect = 0.000e+0
Identity = 460/598 (76.92%), Postives = 518/598 (86.62%), Query Frame = 0
Query: 6 TWAKPKILTSCPQREVKSQKAY-----RKNNVNCSLQSPSILHFPKQSPHIYQTP---CVPTT-----IAQPKHQD-SSSSNQWNFLQKAASMALDAVENVIAAREKEHPLPKTADPQVQIAGNFAPVPEQPVRHRLPVTGKIPECISGVYVRNGANPHLEPTAGHHFFDGDGMVHAVQFKEGAASYACRFTETERLIQERNLGKPVFPKAIGELHGHSGIARLALFYARGLFGLVDHSQGTGVANAGLVYFNNRLLAMSEDDLPYHVEITSSGDLKTVGRYNFDGQLDTTMIAHPKIDPDTKELIALSYNVIQKPYLKYFRVSPDGVKSDDVDIELADPTMMHDFAITENFVVIPDQQVVFKISEMIKGGSPVVYDKNKVSRFGVLDKYAKDGEGIKWVEVPDCFCFHLWNAWEDEESDEIVVIGSCMTPADSIFNECDEGLQSVLSEIRLNLKTGKSTRKPIM-SSEDQVNLEAGMVNKNKLGRKTQFAYLAIAEPWPKVSGFAKVDLFTGQVQKFFYGDHKYGGEPLFLPKNPNSENEDDGYILAFVHDEKAWKSELQIVNAMTLELEATVQLPSRVPYGFHGTFISAKDLESQA 588 TW KPKI + S + + N + CSLQ+P ILHFPKQ YQTP +PT+ I+ PK ++ SSSS+ WN +QKAA+MALDAVE+V+ E EHPLPKTADP+VQI+GNFAPVPE PV LPVTGKIP+C++GVYVRNGANP EPTAGHHFFDGDGMVHAVQFK G+ASYACRFTETERL QE+ LG+PVFPKAIGELHGHSGIARL LFYARG+FGLVDHS GTGVANAGLVYFNNRLLAMSEDDLPYHV +T++GDL+T GR +FDGQL +TMI HPK+DP + EL ALSY+VIQKPYLKYFR S +G KS+DV+I + DPTMMHDFAITE FVVIPDQQVVFK+SEMI GGSPVVYDKNKVSRFG+LDKYAKDG G+KWVEVPDCFCFHLWNAWE+ E+DEIVVIGSCMTP DSIFNECDEGL+SVLSEIRLNLKTGKSTRK I+ + E+QVNLEAGMVN+NKLGRKTQ+AYLAIAEPWPKVSGFAKVDLFTG++ KFFYGD+KYGGEPLFLP++PNS++EDDGYILAFVHDEK WKSELQIVNAMTL LEATV+LPSRVPYGFHGTFI+AKDL +QA Sbjct: 10 TWIKPKISMPSSREFGHSSNSISLLKNKPNKITCSLQAPPILHFPKQQSSNYQTPKTNTIPTSKPTTKISHPKQENKSSSSSPWNLVQKAAAMALDAVESVLTKHELEHPLPKTADPRVQISGNFAPVPENPVTQSLPVTGKIPKCVNGVYVRNGANPLFEPTAGHHFFDGDGMVHAVQFKNGSASYACRFTETERLTQEKALGRPVFPKAIGELHGHSGIARLMLFYARGVFGLVDHSNGTGVANAGLVYFNNRLLAMSEDDLPYHVRVTANGDLETDGRVDFDGQLKSTMIVHPKLDPVSGELFALSYDVIQKPYLKYFRFSKNGEKSNDVEIPVEDPTMMHDFAITEKFVVIPDQQVVFKMSEMILGGSPVVYDKNKVSRFGILDKYAKDGSGLKWVEVPDCFCFHLWNAWEEPETDEIVVIGSCMTPPDSIFNECDEGLESVLSEIRLNLKTGKSTRKAIIQNEEEQVNLEAGMVNRNKLGRKTQYAYLAIAEPWPKVSGFAKVDLFTGEINKFFYGDNKYGGEPLFLPRDPNSKDEDDGYILAFVHDEKEWKSELQIVNAMTLRLEATVKLPSRVPYGFHGTFINAKDLANQA 607
Match Name | Stats | Description |
---|---|---|
Q2VEW9 | E-Value: 0.000e+0, PID: 99.66 | (Putative 9-cis epoxycarotenoid dioxygenase {ECO:0... [more] |
Q2VEW8 | E-Value: 0.000e+0, PID: 87.05 | (Putative 9-cis epoxycarotenoid dioxygenase {ECO:0... [more] |
A0A1U7Y7N0 | E-Value: 0.000e+0, PID: 76.87 | (9-cis-epoxycarotenoid dioxygenase NCED1, chloropl... [more] |
A0A0N7CTG4 | E-Value: 0.000e+0, PID: 76.87 | (9-cis-epoxycarotenoid dioxygenase 3-2 {ECO:000031... [more] |
A0A0A7DW45 | E-Value: 0.000e+0, PID: 76.92 | (9-cis-epoxycarotenoid dioxygenase 1 {ECO:0000313|... [more] |
A0A0N7CUA2 | E-Value: 0.000e+0, PID: 76.69 | (9-cis-epoxycarotenoid dioxygenase 3-1 {ECO:000031... [more] |
A0A2G2WEP9 | E-Value: 0.000e+0, PID: 76.53 | (9-cis-epoxycarotenoid dioxygenase NCED1, chloropl... [more] |
A0A0A7EBR7 | E-Value: 0.000e+0, PID: 76.76 | (9-cis epoxycarotenoid dioxygenase {ECO:0000313|EM... [more] |
W5QL26 | E-Value: 0.000e+0, PID: 76.76 | (9-cis-epoxy-carotenoid dioxygenase {ECO:0000313|E... [more] |
A0A0A7DVZ4 | E-Value: 0.000e+0, PID: 76.92 | (9-cis-epoxycarotenoid dioxygenase 1 {ECO:0000313|... [more] |
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An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions. | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |