Resource Type:
Polypeptide
Name:
DCAR_017658
Identifier:
DCAR_017658.mRNA-protein
Sequence:
MPYHSISSLSLLPIPIRQNLSKHHPPFHQPPHSLPLSIKSSLDNKPPKSN
QGSWVSPDWLTSLTKSLTLSKDDSNIPIASAKLEDVSELLGGALFLPLFK
WMNMYGPIYRLAAGPRDFVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLF
GSGFAIAEGPLWTARRRAVVPSLHKKYLSVMVDRVFCKCAERLVEKLEIS
ALNGSAVNMEEQFSQLTLDVIGLSVFNYNFDSLNADSPVIEAVYTALKEA
EARSTDILPYWKIEALCKIIPRQVKAEKAVTVIRTTVEELIEKCKKIVDT
EGERISEEEYVNEADPSILRFLLASREEVSSTQLRDDLLSMLVAGHETTG
SVLTWTSYLLSKNPSSLVKAQAEVDRVLEGRPPTYDDIKNLKYLTRCINE
SMRLYPHPPVLIRRAEVADELPGSYKVNPGQDIMISVYNIHHSSKVWDRA
EEFIPERFDLDGPVPNESNTDYRFIPFSGGPRKCVGDQFALLEAIVSLAI
FLQHLSFELIPDQVINMTTGATIHTTNGLYMKVSKRQIKSAAVSSISR*
Sequence Length:
549
Sequence Checksum:
33dd6cdf2ce0ba9d8aca2a608d4bf28a
View location in JBrowse:
Organism:
Relationship:
| Relationships |
|---|
| The polypeptide, DCAR_017658, derives from mRNA, DCAR_017658. |
Blast Results:
The following BLAST results are available for this feature:
BLAST of DCAR_017658 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
BLAST of DCAR_017658 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: Q2VEX2 ((Putative epsilon-ring carotene hydroxylase {ECO:0000313|EMBL:ABB52076.1}))
HSP 1 Score: 1126.31 bits (2912), Expect = 0.000e+0
Identity = 547/548 (99.82%), Postives = 548/548 (100.00%), Query Frame = 0
Match: Q2VEX2 ((Putative epsilon-ring carotene hydroxylase {ECO:0000313|EMBL:ABB52076.1}))
HSP 1 Score: 1126.31 bits (2912), Expect = 0.000e+0
Identity = 547/548 (99.82%), Postives = 548/548 (100.00%), Query Frame = 0
Query: 1 MPYHSISSLSLLPIPIRQNLSKHHPPFHQPPHSLPLSIKSSLDNKPPKSNQGSWVSPDWLTSLTKSLTLSKDDSNIPIASAKLEDVSELLGGALFLPLFKWMNMYGPIYRLAAGPRDFVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEGPLWTARRRAVVPSLHKKYLSVMVDRVFCKCAERLVEKLEISALNGSAVNMEEQFSQLTLDVIGLSVFNYNFDSLNADSPVIEAVYTALKEAEARSTDILPYWKIEALCKIIPRQVKAEKAVTVIRTTVEELIEKCKKIVDTEGERISEEEYVNEADPSILRFLLASREEVSSTQLRDDLLSMLVAGHETTGSVLTWTSYLLSKNPSSLVKAQAEVDRVLEGRPPTYDDIKNLKYLTRCINESMRLYPHPPVLIRRAEVADELPGSYKVNPGQDIMISVYNIHHSSKVWDRAEEFIPERFDLDGPVPNESNTDYRFIPFSGGPRKCVGDQFALLEAIVSLAIFLQHLSFELIPDQVINMTTGATIHTTNGLYMKVSKRQIKSAAVSSISR 548
MPYHSISSLSLLPIPIRQNLSKHHPPFHQPPHSLPLSIKSSLDNKPPKSNQGSWVSPDWLTSLTKS+TLSKDDSNIPIASAKLEDVSELLGGALFLPLFKWMNMYGPIYRLAAGPRDFVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEGPLWTARRRAVVPSLHKKYLSVMVDRVFCKCAERLVEKLEISALNGSAVNMEEQFSQLTLDVIGLSVFNYNFDSLNADSPVIEAVYTALKEAEARSTDILPYWKIEALCKIIPRQVKAEKAVTVIRTTVEELIEKCKKIVDTEGERISEEEYVNEADPSILRFLLASREEVSSTQLRDDLLSMLVAGHETTGSVLTWTSYLLSKNPSSLVKAQAEVDRVLEGRPPTYDDIKNLKYLTRCINESMRLYPHPPVLIRRAEVADELPGSYKVNPGQDIMISVYNIHHSSKVWDRAEEFIPERFDLDGPVPNESNTDYRFIPFSGGPRKCVGDQFALLEAIVSLAIFLQHLSFELIPDQVINMTTGATIHTTNGLYMKVSKRQIKSAAVSSISR
Sbjct: 1 MPYHSISSLSLLPIPIRQNLSKHHPPFHQPPHSLPLSIKSSLDNKPPKSNQGSWVSPDWLTSLTKSITLSKDDSNIPIASAKLEDVSELLGGALFLPLFKWMNMYGPIYRLAAGPRDFVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEGPLWTARRRAVVPSLHKKYLSVMVDRVFCKCAERLVEKLEISALNGSAVNMEEQFSQLTLDVIGLSVFNYNFDSLNADSPVIEAVYTALKEAEARSTDILPYWKIEALCKIIPRQVKAEKAVTVIRTTVEELIEKCKKIVDTEGERISEEEYVNEADPSILRFLLASREEVSSTQLRDDLLSMLVAGHETTGSVLTWTSYLLSKNPSSLVKAQAEVDRVLEGRPPTYDDIKNLKYLTRCINESMRLYPHPPVLIRRAEVADELPGSYKVNPGQDIMISVYNIHHSSKVWDRAEEFIPERFDLDGPVPNESNTDYRFIPFSGGPRKCVGDQFALLEAIVSLAIFLQHLSFELIPDQVINMTTGATIHTTNGLYMKVSKRQIKSAAVSSISR 548
BLAST of DCAR_017658 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A286MU89 ((Cytochrome P450 monooxygenase CYP97C {ECO:0000313|EMBL:ASW34031.1}))
HSP 1 Score: 914.064 bits (2361), Expect = 0.000e+0
Identity = 436/525 (83.05%), Postives = 488/525 (92.95%), Query Frame = 0
Match: A0A286MU89 ((Cytochrome P450 monooxygenase CYP97C {ECO:0000313|EMBL:ASW34031.1}))
HSP 1 Score: 914.064 bits (2361), Expect = 0.000e+0
Identity = 436/525 (83.05%), Postives = 488/525 (92.95%), Query Frame = 0
Query: 25 PPFHQPPHSLPLSIKSSLDNKP-PKSNQGSWVSPDWLTSLTKSLTLSK-DDSNIPIASAKLEDVSELLGGALFLPLFKWMNMYGPIYRLAAGPRDFVVVSDPAIAKHVLRNYGK-YAKGLVAEVSEFLFGSGFAIAEGPLWTARRRAVVPSLHKKYLSVMVDRVFCKCAERLVEKLEISALNGSAVNMEEQFSQLTLDVIGLSVFNYNFDSLNADSPVIEAVYTALKEAEARSTDILPYWKIEALCKIIPRQVKAEKAVTVIRTTVEELIEKCKKIVDTEGERISEEEYVNEADPSILRFLLASREEVSSTQLRDDLLSMLVAGHETTGSVLTWTSYLLSKNPSSLVKAQAEVDRVLEGRPPTYDDIKNLKYLTRCINESMRLYPHPPVLIRRAEVADELPGSYKVNPGQDIMISVYNIHHSSKVWDRAEEFIPERFDLDGPVPNESNTDYRFIPFSGGPRKCVGDQFALLEAIVSLAIFLQHLSFELIPDQVINMTTGATIHTTNGLYMKVSKRQIKSAAVSSI 546
PP HQP L+IK SL+NKP P S GSWVSPDWLTSLT+SLT++K D+SNIPIA AKLEDVS+LLGGALFLPLFKWMN YGPIYRLAAGPR+FV+VSDP IAKHVLRNYG YAKGLVAEVSEFLFGSGFAIAEG LWTARRRAVVPSLHKKYLSV+VDRVFCKC+ER V+KL+ ALNG+AVNMEEQFSQLTLDVIGL+VFNYNFDSL +DSPVIE+VYTALKEAEARSTD+LPYWKI+ LCKIIPRQ+KA++AVT+IR TVEELI KCK++V+ EGE+I +E+YVN+ADPSILRFLLASREEV+S QLRDDLLSMLVAGHETTGSVLTWT+YLLSK+PSSLVKAQ EVDRVL+GRPPTY+DIKNLK++TRCINESMRLYPHPPVLIRRA VADELPG+YKVNPGQDIMISVYNIHHSS+VW+RAEEFIPERFDLDGPVPNE+NTDY++IPFSGGPRKCVGDQFA++EAIV+LAIFLQ + FEL+PDQ INMTTGATIHTT+GLYMKV +RQ+ S VSS+
Sbjct: 28 PPKHQPH----LTIKCSLENKPKPNSKNGSWVSPDWLTSLTRSLTITKKDNSNIPIAEAKLEDVSDLLGGALFLPLFKWMNDYGPIYRLAAGPRNFVIVSDPLIAKHVLRNYGTIYAKGLVAEVSEFLFGSGFAIAEGSLWTARRRAVVPSLHKKYLSVIVDRVFCKCSERFVDKLKTYALNGTAVNMEEQFSQLTLDVIGLAVFNYNFDSLTSDSPVIESVYTALKEAEARSTDLLPYWKIDVLCKIIPRQIKAQQAVTIIRETVEELILKCKEMVEKEGEKIDDEDYVNDADPSILRFLLASREEVTSQQLRDDLLSMLVAGHETTGSVLTWTAYLLSKDPSSLVKAQEEVDRVLQGRPPTYEDIKNLKFITRCINESMRLYPHPPVLIRRAIVADELPGNYKVNPGQDIMISVYNIHHSSQVWERAEEFIPERFDLDGPVPNEANTDYKYIPFSGGPRKCVGDQFAMMEAIVALAIFLQKMEFELVPDQKINMTTGATIHTTSGLYMKVKERQVNSMMVSSV 548
BLAST of DCAR_017658 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2R6Q9P8 ((Carotene epsilon-monooxygenase {ECO:0000313|EMBL:PSS04629.1}))
HSP 1 Score: 913.294 bits (2359), Expect = 0.000e+0
Identity = 433/509 (85.07%), Postives = 475/509 (93.32%), Query Frame = 0
Match: A0A2R6Q9P8 ((Carotene epsilon-monooxygenase {ECO:0000313|EMBL:PSS04629.1}))
HSP 1 Score: 913.294 bits (2359), Expect = 0.000e+0
Identity = 433/509 (85.07%), Postives = 475/509 (93.32%), Query Frame = 0
Query: 40 SSLDNKPPKSNQGSWVSPDWLTSLTKSLTLSKDDSNIPIASAKLEDVSELLGGALFLPLFKWMNMYGPIYRLAAGPRDFVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEGPLWTARRRAVVPSLHKKYLSVMVDRVFCKCAERLVEKLEISALNGSAVNMEEQFSQLTLDVIGLSVFNYNFDSLNADSPVIEAVYTALKEAEARSTDILPYWKIEALCKIIPRQVKAEKAVTVIRTTVEELIEKCKKIVDTEGERISEEEYVNEADPSILRFLLASREEVSSTQLRDDLLSMLVAGHETTGSVLTWTSYLLSKNPSSLVKAQAEVDRVLEGRPPTYDDIKNLKYLTRCINESMRLYPHPPVLIRRAEVADELPGSYKVNPGQDIMISVYNIHHSSKVWDRAEEFIPERFDLDGPVPNESNTDYRFIPFSGGPRKCVGDQFALLEAIVSLAIFLQHLSFELIPDQVINMTTGATIHTTNGLYMKVSKRQIKSAAVSSISR 548
SS D KP + SWVSPDW+TSLT+SL +KDDSNIPIA+AKL+DVSELLGGALFLPLFKWM YGPIYRLAAGPRDFVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEG LWT RRRAVVPSLHKKYLSV+VDRVFCKCAERLVEKL+ A+NGSAVNME +F QLTLDVIGL+VFNYNFDSL DSPVI+AVYTALKEAEARSTD+LPYWKI ALCKIIPRQ+KAEKAVTVIR TVEELI KCK+IV++EGERI+EEEYVNE DPSILRFLLASR+EVSSTQLRDDLLSMLVAGHETTGSVLTWT+YLLSK+PSSL KAQ EVDRVL+GRPPTY+DIKNLK+LTRCINES+RLYPHPPVL+RRA+V D LPG+YKVN GQDIMISVYNIHHS++VW+RAEEF+PERFDLD PVPNE+NTD+RFIPFSGGPRKCVGDQFAL+EAIV+LAIFLQH++FEL+PDQ I+MTTGATIHTTNGLYMKVS+RQ +SA SS SR
Sbjct: 72 SSSDPKPTTTKPSSWVSPDWVTSLTRSLNFAKDDSNIPIATAKLDDVSELLGGALFLPLFKWMIQYGPIYRLAAGPRDFVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEGQLWTVRRRAVVPSLHKKYLSVIVDRVFCKCAERLVEKLKTDAINGSAVNMEAKFCQLTLDVIGLAVFNYNFDSLTTDSPVIDAVYTALKEAEARSTDLLPYWKINALCKIIPRQIKAEKAVTVIRRTVEELIAKCKEIVESEGERINEEEYVNETDPSILRFLLASRDEVSSTQLRDDLLSMLVAGHETTGSVLTWTTYLLSKDPSSLKKAQEEVDRVLQGRPPTYEDIKNLKFLTRCINESLRLYPHPPVLLRRAQVPDVLPGNYKVNAGQDIMISVYNIHHSAQVWERAEEFVPERFDLDDPVPNETNTDFRFIPFSGGPRKCVGDQFALMEAIVALAIFLQHMNFELVPDQNISMTTGATIHTTNGLYMKVSQRQTESAFASSSSR 580
BLAST of DCAR_017658 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: F6HKE4 ((Uncharacterized protein {ECO:0000313|EMBL:CCB55366.1}))
HSP 1 Score: 910.212 bits (2351), Expect = 0.000e+0
Identity = 440/546 (80.59%), Postives = 496/546 (90.84%), Query Frame = 0
Match: F6HKE4 ((Uncharacterized protein {ECO:0000313|EMBL:CCB55366.1}))
HSP 1 Score: 910.212 bits (2351), Expect = 0.000e+0
Identity = 440/546 (80.59%), Postives = 496/546 (90.84%), Query Frame = 0
Query: 5 SISSLSLLPIPIRQNLSKHHPPFHQPPH-SLP-LSIKSSLDNKPPKSNQGSWVSPDWLTSLTKSLTLSKDDSNIPIASAKLEDVSELLGGALFLPLFKWMNMYGPIYRLAAGPRDFVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEGPLWTARRRAVVPSLHKKYLSVMVDRVFCKCAERLVEKLEISALNGSAVNMEEQFSQLTLDVIGLSVFNYNFDSLNADSPVIEAVYTALKEAEARSTDILPYWKIEALCKIIPRQVKAEKAVTVIRTTVEELIEKCKKIVDTEGERISEEEYVNEADPSILRFLLASREEVSSTQLRDDLLSMLVAGHETTGSVLTWTSYLLSKNPSSLVKAQAEVDRVLEGRPPTYDDIKNLKYLTRCINESMRLYPHPPVLIRRAEVADELPGSYKVNPGQDIMISVYNIHHSSKVWDRAEEFIPERFDLDGPVPNESNTDYRFIPFSGGPRKCVGDQFALLEAIVSLAIFLQHLSFELIPDQVINMTTGATIHTTNGLYMKVSKRQIKSAAVSSISR 548
S+SS +LL P + + P PP+ SLP LSI S DN P + SWVSPDWLTSL++SLT+ +DDS IP+A+AKL+DVSELLGGALFLPLF+WMNMYGPIYRLAAGPR+FVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEG LWT RRRAVVPSLHK+YLS++VDRVFCKCAERLVE L ALNGSAVNMEE+FSQLTLDVIGLSVFNYNFDSL ADSPVI+AVYTALKEAEARSTD+LPYWK++ALCKIIPRQ+KAE++VTVIR TVEELI KCK+IV+ EGERI E+EYVN++DPSILRFLLASREEVSS QLRDDLLSMLVAGHETTGSVLTWT YLLSKN SSL+KAQ EVDRVL+GR P+Y+DIK+LK+LTRCINESMRLYPHPPVLIRRA+VAD LPG+YKVN GQDIMISVYNIHHSS+VW+RAE+F+PERFDL+GPVPNE+NTD+RFIPFSGGPRKCVGDQFALLEAIV+LAIF+QH++FEL+PDQ I+MTTGATIHTTNGLYMK+++RQI SA V + SR
Sbjct: 6 SLSSFALLTPP-----TFNFKPTTLPPNPSLPYLSITCSSDNNPKPTRSSSWVSPDWLTSLSRSLTMGQDDSGIPMATAKLDDVSELLGGALFLPLFRWMNMYGPIYRLAAGPRNFVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEGELWTVRRRAVVPSLHKRYLSIIVDRVFCKCAERLVENLRTDALNGSAVNMEEKFSQLTLDVIGLSVFNYNFDSLTADSPVIDAVYTALKEAEARSTDLLPYWKVKALCKIIPRQIKAEESVTVIRKTVEELIAKCKEIVEREGERIDEDEYVNDSDPSILRFLLASREEVSSVQLRDDLLSMLVAGHETTGSVLTWTLYLLSKNSSSLMKAQEEVDRVLQGRSPSYEDIKDLKFLTRCINESMRLYPHPPVLIRRAKVADVLPGNYKVNAGQDIMISVYNIHHSSQVWERAEDFLPERFDLEGPVPNETNTDFRFIPFSGGPRKCVGDQFALLEAIVALAIFVQHMNFELVPDQNISMTTGATIHTTNGLYMKLTQRQIPSAFVPTSSR 546
BLAST of DCAR_017658 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A4P9I913 ((Carotene epsilon-monooxygenase {ECO:0000313|EMBL:QCU55161.1}))
HSP 1 Score: 905.59 bits (2339), Expect = 0.000e+0
Identity = 437/524 (83.40%), Postives = 479/524 (91.41%), Query Frame = 0
Match: A0A4P9I913 ((Carotene epsilon-monooxygenase {ECO:0000313|EMBL:QCU55161.1}))
HSP 1 Score: 905.59 bits (2339), Expect = 0.000e+0
Identity = 437/524 (83.40%), Postives = 479/524 (91.41%), Query Frame = 0
Query: 23 HHPPFHQPPHSLP--LSIKSSLDNKPPK-----SNQGSWVSPDWLTSLTKSLTLSKDDSNIPIASAKLEDVSELLGGALFLPLFKWMNMYGPIYRLAAGPRDFVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEGPLWTARRRAVVPSLHKKYLSVMVDRVFCKCAERLVEKLEISALNGSAVNMEEQFSQLTLDVIGLSVFNYNFDSLNADSPVIEAVYTALKEAEARSTDILPYWKIEALCKIIPRQVKAEKAVTVIRTTVEELIEKCKKIVDTEGERISEEEYVNEADPSILRFLLASREEVSSTQLRDDLLSMLVAGHETTGSVLTWTSYLLSKNPSSLVKAQAEVDRVLEGRPPTYDDIKNLKYLTRCINESMRLYPHPPVLIRRAEVADELPGSYKVNPGQDIMISVYNIHHSSKVWDRAEEFIPERFDLDGPVPNESNTDYRFIPFSGGPRKCVGDQFALLEAIVSLAIFLQHLSFELIPDQVINMTTGATIHTTNGLYMKVSKRQIK 539
+ P P +LP LSIKSS+D KP K + Q SWVSPDWLTSL +SL++ KDDSNIPIA+AKLEDVSELLGGALFLPLFKWM YGPIYRLAAGPR+FVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEG LWT RRRAVVPSLHK YLSV+VDRVFCKCAERLVEKL+ +ALNGSAVNMEE FSQLTLDVIGL++FNYNFDSL DSPVI+AVYTALKEAEARSTD+LPYWKI ALCKIIPRQ+KAEKAVT+IR TVEELI KCK+IV++EGERI EEEYVNE DPSILRFLLASREEVSS QLRDDLLSMLVAGHETTGSVLTWT+YLLSK+PSSL KAQ EVDRVL+GR PTY+DIKNLK+LTRCINES+RLYPHPPVL+RRA+VAD LPG+YKVNPGQDIMISVYNIHHS +VWDRAE F+PERFDLDGP+PNE+NTD+RFIPFSGGPRKC+GDQFALLEAIV+LAIF+QH+ FEL+PDQ I+MTTGATIHT NGLYMKVS+RQ K
Sbjct: 24 YKPTIPYPSTNLPQSLSIKSSID-KPEKPTNNTTKQSSWVSPDWLTSLAQSLSIGKDDSNIPIANAKLEDVSELLGGALFLPLFKWMQEYGPIYRLAAGPRNFVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEGQLWTVRRRAVVPSLHKTYLSVIVDRVFCKCAERLVEKLKTNALNGSAVNMEENFSQLTLDVIGLALFNYNFDSLTTDSPVIDAVYTALKEAEARSTDLLPYWKINALCKIIPRQIKAEKAVTLIRKTVEELINKCKEIVESEGERIDEEEYVNETDPSILRFLLASREEVSSIQLRDDLLSMLVAGHETTGSVLTWTAYLLSKDPSSLSKAQEEVDRVLQGRSPTYEDIKNLKFLTRCINESLRLYPHPPVLLRRAQVADVLPGNYKVNPGQDIMISVYNIHHSPQVWDRAEVFVPERFDLDGPMPNETNTDFRFIPFSGGPRKCIGDQFALLEAIVALAIFVQHMDFELVPDQNISMTTGATIHTENGLYMKVSQRQTK 546
BLAST of DCAR_017658 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A4P9I8P6 ((Carotene epsilon-monooxygenase {ECO:0000313|EMBL:QCU55160.1}))
HSP 1 Score: 905.59 bits (2339), Expect = 0.000e+0
Identity = 437/524 (83.40%), Postives = 479/524 (91.41%), Query Frame = 0
Match: A0A4P9I8P6 ((Carotene epsilon-monooxygenase {ECO:0000313|EMBL:QCU55160.1}))
HSP 1 Score: 905.59 bits (2339), Expect = 0.000e+0
Identity = 437/524 (83.40%), Postives = 479/524 (91.41%), Query Frame = 0
Query: 23 HHPPFHQPPHSLP--LSIKSSLDNKPPK-----SNQGSWVSPDWLTSLTKSLTLSKDDSNIPIASAKLEDVSELLGGALFLPLFKWMNMYGPIYRLAAGPRDFVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEGPLWTARRRAVVPSLHKKYLSVMVDRVFCKCAERLVEKLEISALNGSAVNMEEQFSQLTLDVIGLSVFNYNFDSLNADSPVIEAVYTALKEAEARSTDILPYWKIEALCKIIPRQVKAEKAVTVIRTTVEELIEKCKKIVDTEGERISEEEYVNEADPSILRFLLASREEVSSTQLRDDLLSMLVAGHETTGSVLTWTSYLLSKNPSSLVKAQAEVDRVLEGRPPTYDDIKNLKYLTRCINESMRLYPHPPVLIRRAEVADELPGSYKVNPGQDIMISVYNIHHSSKVWDRAEEFIPERFDLDGPVPNESNTDYRFIPFSGGPRKCVGDQFALLEAIVSLAIFLQHLSFELIPDQVINMTTGATIHTTNGLYMKVSKRQIK 539
+ P P +LP LSIKSS+D KP K + Q SWVSPDWLTSL +SL++ KDDSNIPIA+AKLEDVSELLGGALFLPLFKWM YGPIYRLAAGPR+FVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEG LWT RRRAVVPSLHK YLSV+VDRVFCKCAERLVEKL+ +ALNGSAVNMEE FSQLTLDVIGL++FNYNFDSL DSPVI+AVYTALKEAEARSTD+LPYWKI ALCKIIPRQ+KAEKAVT+IR TVEELI KCK+IV++EGERI EEEYVNE DPSILRFLLASREEVSS QLRDDLLSMLVAGHETTGSVLTWT+YLLSK+PSSL KAQ EVDRVL+GR PTY+DIKNLK+LTRCINES+RLYPHPPVL+RRA+VAD LPG+YKVNPGQDIMISVYNIHHS +VWDRAE F+PERFDLDGP+PNE+NTD+RFIPFSGGPRKC+GDQFALLEAIV+LAIF+QH+ FEL+PDQ I+MTTGATIHT NGLYMKVS+RQ K
Sbjct: 24 YKPTIPYPSTNLPQSLSIKSSID-KPEKPTNNTTKQSSWVSPDWLTSLAQSLSIGKDDSNIPIANAKLEDVSELLGGALFLPLFKWMQEYGPIYRLAAGPRNFVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEGQLWTVRRRAVVPSLHKTYLSVIVDRVFCKCAERLVEKLKTNALNGSAVNMEENFSQLTLDVIGLALFNYNFDSLTTDSPVIDAVYTALKEAEARSTDLLPYWKINALCKIIPRQIKAEKAVTLIRKTVEELINKCKEIVESEGERIDEEEYVNETDPSILRFLLASREEVSSIQLRDDLLSMLVAGHETTGSVLTWTAYLLSKDPSSLSKAQEEVDRVLQGRSPTYEDIKNLKFLTRCINESLRLYPHPPVLLRRAQVADVLPGNYKVNPGQDIMISVYNIHHSPQVWDRAEVFVPERFDLDGPMPNETNTDFRFIPFSGGPRKCIGDQFALLEAIVALAIFVQHMDFELVPDQNISMTTGATIHTENGLYMKVSQRQTK 546
BLAST of DCAR_017658 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A0B4SXK9 ((Carotene epsilon-monooxygenase {ECO:0000313|EMBL:AJB84623.1}) (1.14.99.45 {ECO:0000313|EMBL:AJB84623.1}))
HSP 1 Score: 905.205 bits (2338), Expect = 0.000e+0
Identity = 436/524 (83.21%), Postives = 480/524 (91.60%), Query Frame = 0
Match: A0A0B4SXK9 ((Carotene epsilon-monooxygenase {ECO:0000313|EMBL:AJB84623.1}) (1.14.99.45 {ECO:0000313|EMBL:AJB84623.1}))
HSP 1 Score: 905.205 bits (2338), Expect = 0.000e+0
Identity = 436/524 (83.21%), Postives = 480/524 (91.60%), Query Frame = 0
Query: 23 HHPPFHQPPHSLP--LSIKSSLDNKPPK-----SNQGSWVSPDWLTSLTKSLTLSKDDSNIPIASAKLEDVSELLGGALFLPLFKWMNMYGPIYRLAAGPRDFVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEGPLWTARRRAVVPSLHKKYLSVMVDRVFCKCAERLVEKLEISALNGSAVNMEEQFSQLTLDVIGLSVFNYNFDSLNADSPVIEAVYTALKEAEARSTDILPYWKIEALCKIIPRQVKAEKAVTVIRTTVEELIEKCKKIVDTEGERISEEEYVNEADPSILRFLLASREEVSSTQLRDDLLSMLVAGHETTGSVLTWTSYLLSKNPSSLVKAQAEVDRVLEGRPPTYDDIKNLKYLTRCINESMRLYPHPPVLIRRAEVADELPGSYKVNPGQDIMISVYNIHHSSKVWDRAEEFIPERFDLDGPVPNESNTDYRFIPFSGGPRKCVGDQFALLEAIVSLAIFLQHLSFELIPDQVINMTTGATIHTTNGLYMKVSKRQIK 539
+ P P +LP LSIKSS+D KP K + Q SWVSPDWLTSL +SL++ KDDSNIPIA+AKLEDVSELLGGALFLPLFKWM YGPIYRLAAGPR+FVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEG LWT RRRAVVPSLHK YLSV+VDRVFCKCAERLVEKL+ +ALNGSAVNMEE FSQLTLDVIGL++FNYNFDSL DSPVI+AVYT+LKEAEARSTD+LPYWKI ALCKIIPRQ+KAEKAVT+IR TVEELI KCK+IV++EGERI+EEEYVNE DPSILRFLLASREEVSS QLRDDLLSMLVAGHETTGSVLTWT+YLLSK+PSSL KAQ EVDRVL+GR PTY+DIKNLK+LTRCINES+RLYPHPPVL+RRA+VAD LPG+YKVNPGQDIMISVYNIHHS +VWDRAE F+PERFDLDGP+PNE+NTD+RFIPFSGGPRKC+GDQFALLEAIV+LAIF+QH+ FEL+PDQ I+MTTGATIHT NGLYMKVS+RQ K
Sbjct: 24 YKPTIPYPSTNLPKSLSIKSSID-KPEKPTNNTTKQSSWVSPDWLTSLAQSLSIGKDDSNIPIANAKLEDVSELLGGALFLPLFKWMQEYGPIYRLAAGPRNFVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEGQLWTVRRRAVVPSLHKTYLSVIVDRVFCKCAERLVEKLKTNALNGSAVNMEENFSQLTLDVIGLALFNYNFDSLTTDSPVIDAVYTSLKEAEARSTDLLPYWKINALCKIIPRQIKAEKAVTLIRKTVEELINKCKEIVESEGERINEEEYVNETDPSILRFLLASREEVSSIQLRDDLLSMLVAGHETTGSVLTWTAYLLSKDPSSLSKAQEEVDRVLQGRSPTYEDIKNLKFLTRCINESLRLYPHPPVLLRRAQVADVLPGNYKVNPGQDIMISVYNIHHSPQVWDRAEVFVPERFDLDGPMPNETNTDFRFIPFSGGPRKCIGDQFALLEAIVALAIFVQHMDFELVPDQNISMTTGATIHTENGLYMKVSQRQTK 546
BLAST of DCAR_017658 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2U1NYM1 ((Cytochrome P450 superfamily protein {ECO:0000313|EMBL:PWA78609.1}))
HSP 1 Score: 904.434 bits (2336), Expect = 0.000e+0
Identity = 431/523 (82.41%), Postives = 484/523 (92.54%), Query Frame = 0
Match: A0A2U1NYM1 ((Cytochrome P450 superfamily protein {ECO:0000313|EMBL:PWA78609.1}))
HSP 1 Score: 904.434 bits (2336), Expect = 0.000e+0
Identity = 431/523 (82.41%), Postives = 484/523 (92.54%), Query Frame = 0
Query: 29 QPPHSLPLSIKSSLDNKP---PKSNQGSWVSPDWLTSLTKSLT-LSKDDSNIPIASAKLEDVSELLGGALFLPLFKWMNMYGPIYRLAAGPRDFVVVSDPAIAKHVLRNYGK-YAKGLVAEVSEFLFGSGFAIAEGPLWTARRRAVVPSLHKKYLSVMVDRVFCKCAERLVEKLEISALNGSAVNMEEQFSQLTLDVIGLSVFNYNFDSLNADSPVIEAVYTALKEAEARSTDILPYWKIEALCKIIPRQVKAEKAVTVIRTTVEELIEKCKKIVDTEGERISEEEYVNEADPSILRFLLASREEVSSTQLRDDLLSMLVAGHETTGSVLTWTSYLLSKNPSSLVKAQAEVDRVLEGRPPTYDDIKNLKYLTRCINESMRLYPHPPVLIRRAEVADELPGSYKVNPGQDIMISVYNIHHSSKVWDRAEEFIPERFDLDGPVPNESNTDYRFIPFSGGPRKCVGDQFALLEAIVSLAIFLQHLSFELIPDQVINMTTGATIHTTNGLYMKVSKRQIKSAAVSSI 546
+PP ++K SL+NKP PKS GSWVSPDWLTSLT+SLT KD+SNIPIA AKLEDVS+LLGGALFLPLFKWMN YGPIYRLAAGPR+FV+VSDP IAKHVLRNYG YAKGLVAEVSEFLFGSGFAIAEG LWTARRRAVVPSLHKKYLSV+VDRVFCKC+ER V+KL+ ALNG+AVNMEEQFSQLTLDVIGL+VFNYNFDSL +DSPVIE+VYTALKEAEARSTD+LPYWKI+ALCKI+PRQ+KA++AVT+IR TVEELI KCK++V+ EGE+I++E+YVN+ADPSILRFLLASREEV+S QLRDDLLSMLVAGHETTGSVLTWT+YLLSK PSSLVKAQ EVDRVL+GR PTY+DIKNLK++TRCINESMRLYPHPPVLIRRA VADELPG+YKVNPGQDIMISVYNIHHSS+VW+RAEEFIPERFDLDGPVPNE+NTDY++IPFSGGPRKCVGDQFA++EAIV+LAIFLQ + FEL+PDQ INMTTGATIHTT+GLYMKV +RQ+ S VSS+
Sbjct: 24 KPPPKKHFTLKCSLENKPNPKPKSKNGSWVSPDWLTSLTRSLTNTKKDNSNIPIAEAKLEDVSDLLGGALFLPLFKWMNDYGPIYRLAAGPRNFVIVSDPLIAKHVLRNYGTIYAKGLVAEVSEFLFGSGFAIAEGSLWTARRRAVVPSLHKKYLSVIVDRVFCKCSERFVDKLKTYALNGTAVNMEEQFSQLTLDVIGLAVFNYNFDSLTSDSPVIESVYTALKEAEARSTDLLPYWKIDALCKIVPRQIKAQQAVTIIRETVEELILKCKEMVEKEGEKINDEDYVNDADPSILRFLLASREEVTSQQLRDDLLSMLVAGHETTGSVLTWTAYLLSKAPSSLVKAQEEVDRVLQGRLPTYEDIKNLKFITRCINESMRLYPHPPVLIRRAIVADELPGNYKVNPGQDIMISVYNIHHSSQVWERAEEFIPERFDLDGPVPNEANTDYKYIPFSGGPRKCVGDQFAMMEAIVALAIFLQKMEFELVPDQKINMTTGATIHTTSGLYMKVKERQVNSMMVSSV 546
BLAST of DCAR_017658 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A4P9I8K4 ((Carotene epsilon-monooxygenase {ECO:0000313|EMBL:QCU55163.1}))
HSP 1 Score: 903.664 bits (2334), Expect = 0.000e+0
Identity = 436/522 (83.52%), Postives = 478/522 (91.57%), Query Frame = 0
Match: A0A4P9I8K4 ((Carotene epsilon-monooxygenase {ECO:0000313|EMBL:QCU55163.1}))
HSP 1 Score: 903.664 bits (2334), Expect = 0.000e+0
Identity = 436/522 (83.52%), Postives = 478/522 (91.57%), Query Frame = 0
Query: 23 HHPPFHQPPHSLP--LSIKSSLDNKPPK-----SNQGSWVSPDWLTSLTKSLTLSKDDSNIPIASAKLEDVSELLGGALFLPLFKWMNMYGPIYRLAAGPRDFVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEGPLWTARRRAVVPSLHKKYLSVMVDRVFCKCAERLVEKLEISALNGSAVNMEEQFSQLTLDVIGLSVFNYNFDSLNADSPVIEAVYTALKEAEARSTDILPYWKIEALCKIIPRQVKAEKAVTVIRTTVEELIEKCKKIVDTEGERISEEEYVNEADPSILRFLLASREEVSSTQLRDDLLSMLVAGHETTGSVLTWTSYLLSKNPSSLVKAQAEVDRVLEGRPPTYDDIKNLKYLTRCINESMRLYPHPPVLIRRAEVADELPGSYKVNPGQDIMISVYNIHHSSKVWDRAEEFIPERFDLDGPVPNESNTDYRFIPFSGGPRKCVGDQFALLEAIVSLAIFLQHLSFELIPDQVINMTTGATIHTTNGLYMKVSKRQ 537
+ P P +LP LSIKSS+D KP K + Q SWVSPDWLTSL +SL++ KDDSNIPIA+AKLEDVSELLGGALFLPLFKWM YGPIYRLAAGPR+FVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEG LWT RRRAVVPSLHK YLSV+VDRVFCKCAERLVEKL+ +ALNGSAVNMEE FSQLTLDVIGL++FNYNFDSL DSPVI+AVYTALKEAEARSTD+LPYWKI ALCKIIPRQ+KAEKAVT+IR TVEELI KCK+IV++EGERI EEEYVNE DPSILRFLLASREEVSS QLRDDLLSMLVAGHETTGSVLTWT+YLLSK+PSSL KAQ EVDRVL+GR PTY+DIKNLK+LTRCINES+RLYPHPPVL+RRA+VAD LPG+YKVNPGQDIMISVYNIHHS +VWDRAE F+PERFDLDGP+PNE+NTD+RFIPFSGGPRKC+GDQFALLEAIV+LAIF+QH+ FEL+PDQ I+MTTGATIHT NGLYMKVS+RQ
Sbjct: 24 YKPTIPYPSTNLPQSLSIKSSID-KPEKPTNNTTKQSSWVSPDWLTSLAQSLSIGKDDSNIPIANAKLEDVSELLGGALFLPLFKWMQEYGPIYRLAAGPRNFVVVSDPAIAKHVLRNYGKYAKGLVAEVSEFLFGSGFAIAEGQLWTVRRRAVVPSLHKTYLSVIVDRVFCKCAERLVEKLKTNALNGSAVNMEENFSQLTLDVIGLALFNYNFDSLTTDSPVIDAVYTALKEAEARSTDLLPYWKINALCKIIPRQIKAEKAVTLIRKTVEELINKCKEIVESEGERIDEEEYVNETDPSILRFLLASREEVSSIQLRDDLLSMLVAGHETTGSVLTWTAYLLSKDPSSLSKAQEEVDRVLQGRSPTYEDIKNLKFLTRCINESLRLYPHPPVLLRRAQVADVLPGNYKVNPGQDIMISVYNIHHSPQVWDRAEVFVPERFDLDGPMPNETNTDFRFIPFSGGPRKCIGDQFALLEAIVALAIFVQHMDFELLPDQNISMTTGATIHTENGLYMKVSQRQ 544
BLAST of DCAR_017658 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5N6P4M1 (PHD-type domain-containing protein {ECO:0000259|PROSITE:PS50016})
HSP 1 Score: 902.894 bits (2332), Expect = 0.000e+0
Identity = 437/552 (79.17%), Postives = 491/552 (88.95%), Query Frame = 0
Match: A0A5N6P4M1 (PHD-type domain-containing protein {ECO:0000259|PROSITE:PS50016})
HSP 1 Score: 902.894 bits (2332), Expect = 0.000e+0
Identity = 437/552 (79.17%), Postives = 491/552 (88.95%), Query Frame = 0
Query: 7 SSLSLLPIPIRQNLSKHHPPFHQPPHS-------LPLSIKSSLDNKP---PKSNQGSWVSPDWLTSLTKSLTLSK-DDSNIPIASAKLEDVSELLGGALFLPLFKWMNMYGPIYRLAAGPRDFVVVSDPAIAKHVLRNYGK-YAKGLVAEVSEFLFGSGFAIAEGPLWTARRRAVVPSLHKKYLSVMVDRVFCKCAERLVEKLEISALNGSAVNMEEQFSQLTLDVIGLSVFNYNFDSLNADSPVIEAVYTALKEAEARSTDILPYWKIEALCKIIPRQVKAEKAVTVIRTTVEELIEKCKKIVDTEGERISEEEYVNEADPSILRFLLASREEVSSTQLRDDLLSMLVAGHETTGSVLTWTSYLLSKNPSSLVKAQAEVDRVLEGRPPTYDDIKNLKYLTRCINESMRLYPHPPVLIRRAEVADELPGSYKVNPGQDIMISVYNIHHSSKVWDRAEEFIPERFDLDGPVPNESNTDYRFIPFSGGPRKCVGDQFALLEAIVSLAIFLQHLSFELIPDQVINMTTGATIHTTNGLYMKVSKRQIKSAAVSSI 546
S LSL P P+ H P PP S P +IKSSL+N P P S GSWVSPDWLT+L +S++ +K DDSNIPIASA+L+DVS+LLGGALFLPLFKWMN YGPIYRLAAGPR+FV+VSDPAIAKHVLRNYG YAKGLVAEVSEFLFG+GFAIAEG LWTARRRAVVPSLHKKYLSV+VDRVFCKC+ERLVEKL+ N + VNME+QFSQLTLDVIGL+VFNYNFDSL ADSPVIE+VYTALKEAEARSTD+LPYWKI ALCKIIPRQ+KAE+AVTVIR TVEELI KCK+IV+ +GE+I +E+YVN+ADPSILRFLLASREEVSS QLRDDLLSMLVAGHETTGSVLTWT+YLLSK+ SSL+KAQ EVDRVL+GR PTY+DIKNLK++TRCINESMRLYPHPPVLIRRA VADELPG+YKVNPGQDIMISVYNIHHSS+VW+RAEEFIPERF LDGPVPNE+NTDY+FIPFSGGPRKCVGDQFA+LEAIV+LAIFLQ++ FEL+P+Q INMTTGATIHTT+GLYMKV +R++ S AVSS+
Sbjct: 3 SVLSLSPSPLVTTTHHHRLP---PPKSSLHITTHRPFTIKSSLENNPKPKPTSKNGSWVSPDWLTALVRSISFTKSDDSNIPIASAQLDDVSDLLGGALFLPLFKWMNDYGPIYRLAAGPRNFVIVSDPAIAKHVLRNYGSIYAKGLVAEVSEFLFGTGFAIAEGSLWTARRRAVVPSLHKKYLSVIVDRVFCKCSERLVEKLKSYECNDTPVNMEQQFSQLTLDVIGLAVFNYNFDSLTADSPVIESVYTALKEAEARSTDLLPYWKISALCKIIPRQIKAEQAVTVIRETVEELIMKCKEIVEKQGEKIDDEDYVNDADPSILRFLLASREEVSSQQLRDDLLSMLVAGHETTGSVLTWTTYLLSKDQSSLMKAQEEVDRVLQGRNPTYEDIKNLKFITRCINESMRLYPHPPVLIRRAIVADELPGNYKVNPGQDIMISVYNIHHSSEVWERAEEFIPERFGLDGPVPNETNTDYKFIPFSGGPRKCVGDQFAMLEAIVALAIFLQNIEFELVPNQKINMTTGATIHTTSGLYMKVKERKVNSMAVSSV 551
| Match Name | Stats | Description |
|---|---|---|
| Q2VEX2 | E-Value: 0.000e+0, PID: 99.82 | (Putative epsilon-ring carotene hydroxylase {ECO:0... [more] |
| A0A286MU89 | E-Value: 0.000e+0, PID: 83.05 | (Cytochrome P450 monooxygenase CYP97C {ECO:0000313... [more] |
| A0A2R6Q9P8 | E-Value: 0.000e+0, PID: 85.07 | (Carotene epsilon-monooxygenase {ECO:0000313|EMBL:... [more] |
| F6HKE4 | E-Value: 0.000e+0, PID: 80.59 | (Uncharacterized protein {ECO:0000313|EMBL:CCB5536... [more] |
| A0A4P9I913 | E-Value: 0.000e+0, PID: 83.40 | (Carotene epsilon-monooxygenase {ECO:0000313|EMBL:... [more] |
| A0A4P9I8P6 | E-Value: 0.000e+0, PID: 83.40 | (Carotene epsilon-monooxygenase {ECO:0000313|EMBL:... [more] |
| A0A0B4SXK9 | E-Value: 0.000e+0, PID: 83.21 | (Carotene epsilon-monooxygenase {ECO:0000313|EMBL:... [more] |
| A0A2U1NYM1 | E-Value: 0.000e+0, PID: 82.41 | (Cytochrome P450 superfamily protein {ECO:0000313|... [more] |
| A0A4P9I8K4 | E-Value: 0.000e+0, PID: 83.52 | (Carotene epsilon-monooxygenase {ECO:0000313|EMBL:... [more] |
| A0A5N6P4M1 | E-Value: 0.000e+0, PID: 79.17 | PHD-type domain-containing protein {ECO:0000259|PR... [more] |
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back to topAnalysis:
| Name | Description |
|---|---|
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions. | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |