DCAR_022385

Resource Type: 
Polypeptide
Name: 
DCAR_022385
Identifier: 
DCAR_022385.mRNA-protein
Sequence: 
MGVTEHEKSEQVERIKDVVIVNPKPSKGFTSKAIDWIESLIVKLMYDASV
PHHWLAGNFAPVDETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGY
HWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDL
KGLFGLLMVNMQMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKP
YAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPP
YITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKE
MVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFHNANAWE
EGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQK
KLSASAVDFPRINESYTGRKSAANIIDAKTMSPDPVAVVELPHRVPYGFH
AFFVTETSNEILIQPA*
Sequence Length: 
467
Sequence Checksum: 
b86ce674d15a475cb64365a6eccbfada
View location in JBrowse: 
Relationship: 
There is 1 relationship.
Relationships
The polypeptide, DCAR_022385, derives from mRNA, DCAR_022385.
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Blast Results: 
The following BLAST results are available for this feature:
BLAST of DCAR_022385 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Analysis Date: 2021-12-09
Analysis Name: DCAR peptide blastp to SwissProt and TrEMBL without DCAR
Total hits: 20
BLAST of DCAR_022385 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: Q2VEW7 ((Putative carotenoid cleavage dioxygenase {ECO:0000313|EMBL:ABB52081.1}))

HSP 1 Score: 853.973 bits (2205), Expect = 0.000e+0
Identity = 412/421 (97.86%), Postives = 413/421 (98.10%), Query Frame = 0
 
Query:    1 MGVTEHEKSEQVERIKDVVIVNPKPSKGFTSKAIDWIESLIVKLMYDASVPHHWLAGNFAPVDETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGYHWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKKLSASAVDFPRINESYTGRKS 421
            MGVTEHEKSEQVERIKDVVIVNPKPSKG TSKAIDWIESLIVKLMYDASVPHHWLAGNFAPVDETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGYHW+      MIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKKLSASAVDFPRINESYTGRK 
Sbjct:    1 MGVTEHEKSEQVERIKDVVIVNPKPSKGLTSKAIDWIESLIVKLMYDASVPHHWLAGNFAPVDETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGYHWF--DGDGMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKKLSASAVDFPRINESYTGRKQ 419    

HSP 2 Score: 80.1073 bits (196), Expect = 1.847e-13
Identity = 39/45 (86.67%), Postives = 42/45 (93.33%), Query Frame = 0
 
Query:  412 INESYTGRKSAANIIDAKTMSPDPVAVVELPHRVPYGFHAFFVTE 456
            +++  TG KSAANIIDAKTMSPDPVAVVELPHRVPYGFHAFFVTE
Sbjct:  495 LHDESTG-KSAANIIDAKTMSPDPVAVVELPHRVPYGFHAFFVTE 538    
BLAST of DCAR_022385 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A7J6F6N7 ((Uncharacterized protein {ECO:0000313|EMBL:KAF4365539.1}))

HSP 1 Score: 788.875 bits (2036), Expect = 0.000e+0
Identity = 375/450 (83.33%), Postives = 413/450 (91.78%), Query Frame = 0
 
Query:   19 VIVNPKPSKGFTSKAIDWIESLIVKLMYDASVPHHWLAGNFAPV-DETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGYHWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFHN-----------ANAWEEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKKLSASAVDFPRINESYTGRKSAANIIDAKTMSPDPVAVVELPHRVPYGFHAFFVTE 456
            V VNPKP+KG +SK +D++ESLIVK MYD+S P H+L+GNFAP+ DETPP +DLPVIG +PECLNGEFVRVGPNPKFSPVAGYHW+      MIHGLRIKDGKATYVSRYVRTSR+KQEE FGG+KFMKIGDLKG FGLLMVNMQMLR KL VLD+SYGNGTANTA+IYHHG+LLALSEADKPY +KVLEDGDLQT+GLLDYDKRLTHSFTAHPKVDPFTGEMFTFGY+HTPPYITYRVISKDG M++PVPIT++DPIMMHDFAITENYAI MDLPL FKPKEMVKEKKLIF+FDATKKARFGVLPRYAK+ELLIKWFELPNCFIFHN           +NAWEE DEVVLITCRLENPDLDMV+G++KEKLENF+NELYEMRFNMK+GLA+QK+LSASAVDFPR+NESYTGRKSA ++IDAKTMS DPVAVV+LPHRVPYGFHAFFVTE
Sbjct:   21 VSVNPKPNKGLSSKLVDFLESLIVKFMYDSSQPQHYLSGNFAPIRDETPPIKDLPVIGYLPECLNGEFVRVGPNPKFSPVAGYHWF--DGDGMIHGLRIKDGKATYVSRYVRTSRIKQEEYFGGSKFMKIGDLKGFFGLLMVNMQMLRAKLKVLDLSYGNGTANTALIYHHGKLLALSEADKPYVLKVLEDGDLQTIGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYAHTPPYITYRVISKDGFMHEPVPITVSDPIMMHDFAITENYAIFMDLPLIFKPKEMVKEKKLIFSFDATKKARFGVLPRYAKDELLIKWFELPNCFIFHNGAHSSLSTFYVSNAWEEEDEVVLITCRLENPDLDMVSGTVKEKLENFSNELYEMRFNMKTGLATQKQLSASAVDFPRVNESYTGRKSAVHVIDAKTMSSDPVAVVDLPHRVPYGFHAFFVTE 468    

HSP 2 Score: 553.132 bits (1424), Expect = 0.000e+0
Identity = 276/461 (59.87%), Postives = 326/461 (70.72%), Query Frame = 0
 
Query:   15 IKDVVIVNPKPSKGFTSKAIDWIESLIVKLMYDASVPHHWLAGNFAPVDETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGYHWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMS---------------YGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKKLSASAVDFPRINESYTGRKSAANIIDAKTMSPDPVAVVELPHRVPYGFHAFFVTETSNE 460
            +K +V VNPKP+KG TSK IDW E++IVKLMYD+S   H+L+GNF  V ETPP +DLPV+G +PECLNGEFVRVG N KF+PVAGYHW+      M+HG+RIKDGKATYVSRYV+TSRLKQEE FGG+KF+K+GDLKGLFGL M ++Q LR K    D+                Y +  ANTA++YHHG+LL L EADKPY +KVLEDGDLQTLG+LDYDKRL H+FTAHPKVDPFTGEMFTFGYS +PPY+TYRVISKDG M+D                                 +EMV E KLI +FDATKKARFGVLPRYAK+ELLIKWFELP+C+IFHNANAWEE DEVVLITCRL+N D++M++G +K+KL +F  ELYE                                KS  ++IDAKTMS DP+AV+ LP RVPYGFHAFFVTE SN+
Sbjct:  536 VKGIVTVNPKPNKGLTSKLIDWFENVIVKLMYDSSQHQHYLSGNFTSVPETPPIKDLPVMGYLPECLNGEFVRVGSNSKFAPVAGYHWF--DGDGMVHGVRIKDGKATYVSRYVKTSRLKQEEYFGGSKFLKVGDLKGLFGLFMFSIQTLRDKFKEADIYSDTFLPLVMEGLIVFYHSLPANTALVYHHGKLLVLHEADKPYVLKVLEDGDLQTLGMLDYDKRLAHTFTAHPKVDPFTGEMFTFGYSLSPPYVTYRVISKDGFMHD---------------------------------QEMVMENKLILSFDATKKARFGVLPRYAKDELLIKWFELPSCYIFHNANAWEEEDEVVLITCRLDNIDMNMISGEIKKKLNHFRTELYE--------------------------------KSEVHVIDAKTMSADPIAVIHLPCRVPYGFHAFFVTEVSNQ 929    

HSP 3 Score: 317.005 bits (811), Expect = 8.598e-94
Identity = 190/384 (49.48%), Postives = 228/384 (59.38%), Query Frame = 0
 
Query:   78 PECLNGEFVRVGPNPKFSPVAGYHWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFH-NANAWEEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKKLSASAVDFPRINESYTGRKSAANIIDAKTMSPDPVAVVELPHRVPYGFHAFFVTETSNE 460
            P+CL G   R        P             MIHGLRIK+GKATY SRYVRTSRLKQEE FGG KFMKIGD+ G+FGLL+V +Q+LR  L VLD+SYG GT                             G++ T G       +TH                        PYITYRVISKDG M+DP+PIT+ +PIMMHDFAIT+NYAI MDLPL F+P+EM+K K  I +FDATKKARFGVLPRYAK+ELLIKWFELPNCFI H  A+  EE DEVV+ITCR+EN DL M  G +K+KL N N  L          L  +  +S              G++   ++IDAKTMS +P+AV++LP RVPYG HA F+TE + E
Sbjct:  932 PKCLFGTTTRTSRALNCEP-----------NLMIHGLRIKNGKATYASRYVRTSRLKQEEYFGGPKFMKIGDMNGVFGLLVVIIQILRAILKVLDLSYGLGT-----------------------------GEMFTFGF----SLITH------------------------PYITYRVISKDGFMHDPIPITIPNPIMMHDFAITQNYAIFMDLPLIFRPEEMMKGKNFIISFDATKKARFGVLPRYAKDELLIKWFELPNCFISHIGAHFSEEDDEVVMITCRIENLDLSMYGGDIKQKL-NINASLSPWVLTTADQLEEEAGVSGG------------GQRCNMHVIDAKTMSANPIAVIDLPCRVPYGLHALFITELTQE 1234    

HSP 4 Score: 283.878 bits (725), Expect = 5.305e-82
Identity = 135/196 (68.88%), Postives = 162/196 (82.65%), Query Frame = 0
 
Query:    6 HEKSEQVERIKDVVIVNPKPSKGFTSKAIDWIESLIVKLMYDASVPHHWLAGNFAPV-DETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGYHWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKP 200
            +E+ E V R   VV VNP P+KGF+S  ++W E+LIV+ MY++S  HH+L GN+APV  ETPP  +LP++G +P+CLNGEFVRVGPNPKF+P+AG+HW+      MIHGLRIKDGKATYVSRYVRTSR+KQEE F  AKF KIGDLKG+FGLLMVN+QMLR KL VLD+SYGNGTANTA+ YHHG+LLALSE DKP
Sbjct: 1246 YEEDEGVSRY-GVVCVNPNPNKGFSSMLLNWFENLIVRFMYNSSSSHHFLTGNYAPVLRETPPVDNLPLLGYLPKCLNGEFVRVGPNPKFAPIAGFHWF--DGDGMIHGLRIKDGKATYVSRYVRTSRIKQEEYFERAKFTKIGDLKGIFGLLMVNIQMLRAKLKVLDLSYGNGTANTALTYHHGKLLALSEVDKP 1438    
BLAST of DCAR_022385 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A660KPA2 ((Uncharacterized protein {ECO:0000313|EMBL:KAE8038242.1}))

HSP 1 Score: 783.097 bits (2021), Expect = 0.000e+0
Identity = 371/444 (83.56%), Postives = 408/444 (91.89%), Query Frame = 0
 
Query:   18 VVIVNPKPSKGFTSKAIDWIESLIVKLMYDASVPHHWLAGNFAPV-DETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGYHWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKKLSASAVDFPRINESYTGRKSAANIIDAKTMSPDPVAVVELPHRVPYGFHAFFVTETSNE 460
            VV+V PKP  G +SK +D++E LIVK M D S P H+LAGNFAPV +ETPPA DL V G +P+CLNGEFVRVGPNPKFSPVAGYHW+      MIHGLRIKDGKATYVSR+VRTSRLKQEE FGGAKFMKIGDLKGLFGLLMV+MQMLR KL VLD+SYG GTANTA+IYHHG+LLALSEADKPY +KVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDG M+DPVPIT+++PIMMHDFAITENYAI MDLPLYF+PKEMVKEKKLIFTFDATKKARFGVLPRYAK++LLI+WFELPNCFIFHNANAWEE DEVVLITCR+EN DLDMV+G++KEK+ENF NELYEMRFNMK+GLASQKKLSASAVDFPR+NESYTGRKSA +++DAKTMS DPVA+VELPHRVPYGFHAFFV+E + E
Sbjct:   16 VVVVEPKPRNGLSSKVVDFVEKLIVKFMLDTSQPQHYLAGNFAPVTEETPPATDLLVKGFLPDCLNGEFVRVGPNPKFSPVAGYHWF--DGDGMIHGLRIKDGKATYVSRFVRTSRLKQEEYFGGAKFMKIGDLKGLFGLLMVHMQMLRAKLKVLDVSYGTGTANTALIYHHGKLLALSEADKPYVLKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGFMHDPVPITISEPIMMHDFAITENYAIFMDLPLYFRPKEMVKEKKLIFTFDATKKARFGVLPRYAKDDLLIRWFELPNCFIFHNANAWEEEDEVVLITCRIENLDLDMVSGTVKEKIENFGNELYEMRFNMKTGLASQKKLSASAVDFPRVNESYTGRKSAVHVLDAKTMSTDPVAIVELPHRVPYGFHAFFVSEVAAE 457    
BLAST of DCAR_022385 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2H5NBG6 ((Uncharacterized protein {ECO:0000313|EMBL:GAY37005.1}))

HSP 1 Score: 780.015 bits (2013), Expect = 0.000e+0
Identity = 368/444 (82.88%), Postives = 406/444 (91.44%), Query Frame = 0
 
Query:   18 VVIVNPKPSKGFTSKAIDWIESLIVKLMYDASVPHHWLAGNFAPV-DETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGYHWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKKLSASAVDFPRINESYTGRKSAANIIDAKTMSPDPVAVVELPHRVPYGFHAFFVTETSNE 460
            +V V+PKP  G TSK ID++E LIVK MYD+S P H+L+GNFAPV DETPP +DL VIG +P+CLNGEFVRVGPNPKF+PVAGYHW+      MIHGLRIKDGKATYVSR+VRTSRLKQEE FGGAKFMKIGDLKGLFGLLMVNMQMLR KL VLD+SYGNGTANTA++YHHG+LLALSEADKPYA+KVLEDGDLQTLG+LDYDKRL HSFTAHPKVDP+TGEMFTFGY+HTPPYITYRVISKDG M+DPVPIT++DPIMMHDFAITENYAI MDLPLYF+PKEMVKE KLIFTFD TKKARFG+LPRYAKNE  +KWFELPNCFIFHNANAWEE DEVVLITCRL+ PDLDMVNG++KEKLENF+NELYEMRFN+K+GLASQK+LSASAVDFPR+NE YTGRKS+ N+IDAKTMS DPVAVVELP RVPYGFHAFFV+E   E
Sbjct:  533 IVAVDPKPKNGVTSKVIDFVEKLIVKWMYDSSQPLHYLSGNFAPVPDETPPTKDLHVIGHLPDCLNGEFVRVGPNPKFAPVAGYHWF--DGDGMIHGLRIKDGKATYVSRFVRTSRLKQEEFFGGAKFMKIGDLKGLFGLLMVNMQMLRAKLKVLDVSYGNGTANTALVYHHGKLLALSEADKPYAVKVLEDGDLQTLGMLDYDKRLQHSFTAHPKVDPYTGEMFTFGYAHTPPYITYRVISKDGFMHDPVPITVSDPIMMHDFAITENYAIFMDLPLYFRPKEMVKENKLIFTFDDTKKARFGILPRYAKNEAQMKWFELPNCFIFHNANAWEEEDEVVLITCRLDKPDLDMVNGAVKEKLENFSNELYEMRFNLKTGLASQKRLSASAVDFPRVNECYTGRKSSVNVIDAKTMSADPVAVVELPQRVPYGFHAFFVSEEQLE 974    

HSP 2 Score: 635.18 bits (1637), Expect = 0.000e+0
Identity = 312/449 (69.49%), Postives = 358/449 (79.73%), Query Frame = 0
 
Query:   18 VVIVNPKPSKGFTSKAIDWIESLIVKLMYDASVPHHWLAGNFAPV-DETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGYHWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFHNANAW-EEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKKLSASAVDFPRINESYTGRKSAANIIDAKTMSPDPVAVVELPHRVPYGFHAFFVTETSNEILIQ 464
            +V+VNPKP KG  +K ID +E LIVK  +D+S P H+L+GNF PV  ETPP  DLPVIG +PECLNGEF+RVGPN KF+PVAGYHW+      M+HGLRIKDGKA YVSRYV+TSRLKQEE FGGAKF KIGD+KGLFGLLMVNM +LR    VLD SYGNG                  AD    +KVL+DGDLQTLG+LDYDKRL H FTAHPK+DP+T EMFTFGY+ TPPY+TYRVISKDG M+DPVPIT++ PIMMHDFAITENYAI +DLP+ F+ KEMVKE KLI+ FD T KARFGVLPRYA+++L I+WFELPNC+IFHNANAW EE +EVVLITCR+ NPDLDM NG +KE LE+   ELYEMRFNMK+GLASQKKLS + VDFPRINE Y GRKSA  +IDAKTMS DPVAV+ELPHRVPYGFH  F++E   E L +
Sbjct:   65 IVVVNPKPQKGLAAKVIDSLEGLIVKFKHDSSRPLHYLSGNFGPVRHETPPTADLPVIGFLPECLNGEFIRVGPNSKFAPVAGYHWF--DGDGMLHGLRIKDGKAAYVSRYVKTSRLKQEEFFGGAKFTKIGDVKGLFGLLMVNMTLLRESFKVLDFSYGNG------------------AD---VLKVLKDGDLQTLGMLDYDKRLNHPFTAHPKIDPYTDEMFTFGYTQTPPYVTYRVISKDGFMHDPVPITMSAPIMMHDFAITENYAIFLDLPMCFRQKEMVKENKLIYAFDPTIKARFGVLPRYARDDLQIRWFELPNCYIFHNANAWEEEEEEVVLITCRVRNPDLDMFNGPLKETLEDQTTELYEMRFNMKTGLASQKKLSPACVDFPRINEHYMGRKSAVYVIDAKTMSADPVAVIELPHRVPYGFHGIFLSEEQLEELAK 490    
BLAST of DCAR_022385 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A4P1RC87 ((Uncharacterized protein {ECO:0000313|EMBL:OIW07986.1}))

HSP 1 Score: 778.859 bits (2010), Expect = 0.000e+0
Identity = 364/450 (80.89%), Postives = 411/450 (91.33%), Query Frame = 0
 
Query:   10 EQVERIKDVVIVNPKPSKGFTSKAIDWIESLIVKLMYDASVPHHWLAGNFAPV-DETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGYHWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKKLSASAVDFPRINESYTGRKSAANIIDAKTMSPDPVAVVELPHRVPYGFHAFFVTETS 458
            E+ ++  ++V V PKPSKGF+SK ID +E ++VKL YD+S+PHH+LAGNFAPV DETPP +DLPVIG +P+CLNGEFVRVGPNPKFSPVAGYHW+      MIHGLR+KDGKATYVSR+VRTSRLKQEE FGG+KFMKIGDLKGLFGLLMVNMQ+LR K  VLD+SYGN TANTA++YHHG+LLALSEADKPY IKV+EDGDLQTLGLLDYDKRL+HSFTAHPKVDPFTGEMFTFGYSHTPPY+TYRVISKDG M+DPVPIT+ +P+MMHDFAITENYAI MDLPLYF+PK+MVK+  LIFTFD+TKKARFGVLPRYAK+E  I+WFELPNCFIFHNANAWEE DEVVLITCR+ENPDLDMVNG+ KEKLENF+NELYEMRFN+K+G ASQKKLSASAVDFPR+NESYTGRKS  ++IDAKTMS DPVA++ELPHRVPYGFHAFFVTE  
Sbjct:    2 EEEKKSSEIVKVEPKPSKGFSSKVIDLLEKVVVKLFYDSSLPHHYLAGNFAPVPDETPPTKDLPVIGYLPDCLNGEFVRVGPNPKFSPVAGYHWF--DGDGMIHGLRVKDGKATYVSRFVRTSRLKQEEYFGGSKFMKIGDLKGLFGLLMVNMQILRAKFKVLDISYGNSTANTALVYHHGKLLALSEADKPYVIKVIEDGDLQTLGLLDYDKRLSHSFTAHPKVDPFTGEMFTFGYSHTPPYVTYRVISKDGYMHDPVPITVPEPVMMHDFAITENYAIFMDLPLYFRPKDMVKKNTLIFTFDSTKKARFGVLPRYAKDEKHIRWFELPNCFIFHNANAWEEEDEVVLITCRIENPDLDMVNGAAKEKLENFSNELYEMRFNIKTGEASQKKLSASAVDFPRVNESYTGRKSFVHVIDAKTMSADPVAIIELPHRVPYGFHAFFVTEVC 449    
BLAST of DCAR_022385 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A2H5N9V9 ((Uncharacterized protein {ECO:0000313|EMBL:GAY37003.1}))

HSP 1 Score: 766.148 bits (1977), Expect = 0.000e+0
Identity = 367/463 (79.27%), Postives = 405/463 (87.47%), Query Frame = 0
 
Query:   18 VVIVNPKPSKGFTSKAIDWIESLIVKLMYDASVPHHWLAGNFAPV-DETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGYHWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMSYGNGT-------------------ANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKKLSASAVDFPRINESYTGRKSAANIIDAKTMSPDPVAVVELPHRVPYGFHAFFVTETSNE 460
            +V V+PKP  G TSK ID++E LIVK MYD+S P H+L+GNFAPV DETPP +DL VIG +P+CLNGEFVRVGPNPKF+PVAGYHW+      MIHGLRIKDGKATYVSR+VRTSRLKQEE FGGAKFMKIGDLKGLFGLLMVNMQMLR KL VLD+SYGNGT                    NTA++YHHG+LLALSEADKPYA+KVLEDGDLQTLG+LDYDKRL HSFTAHPKVDP+TGEMFTFGY+HTPPYITYRVISKDG M+DPVPIT++DPIMMHDFAITENYAI MDLPLYF+PKEMVKE KLIFTFD TKKARFG+LPRYAKNE  +KWFELPNCFIFHNANAWEE DEVVLITCRL+ PDLDMVNG++KEKLENF+NELYEMRFN+K+GLASQK+LSASAVDFPR+NE YTGRKS+ N+IDAKTMS DPVAVVELP RVPYGFHAFFV+E   E
Sbjct:  533 IVAVDPKPKNGVTSKVIDFVEKLIVKWMYDSSQPLHYLSGNFAPVPDETPPTKDLHVIGHLPDCLNGEFVRVGPNPKFAPVAGYHWF--DGDGMIHGLRIKDGKATYVSRFVRTSRLKQEEFFGGAKFMKIGDLKGLFGLLMVNMQMLRAKLKVLDVSYGNGTDTFRLSVQSSYPWKFLQFRTNTALVYHHGKLLALSEADKPYAVKVLEDGDLQTLGMLDYDKRLQHSFTAHPKVDPYTGEMFTFGYAHTPPYITYRVISKDGFMHDPVPITVSDPIMMHDFAITENYAIFMDLPLYFRPKEMVKENKLIFTFDDTKKARFGILPRYAKNEAQMKWFELPNCFIFHNANAWEEEDEVVLITCRLDKPDLDMVNGAVKEKLENFSNELYEMRFNLKTGLASQKRLSASAVDFPRVNECYTGRKSSVNVIDAKTMSADPVAVVELPQRVPYGFHAFFVSEEQLE 993    

HSP 2 Score: 634.795 bits (1636), Expect = 0.000e+0
Identity = 312/449 (69.49%), Postives = 358/449 (79.73%), Query Frame = 0
 
Query:   18 VVIVNPKPSKGFTSKAIDWIESLIVKLMYDASVPHHWLAGNFAPV-DETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGYHWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFHNANAW-EEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKKLSASAVDFPRINESYTGRKSAANIIDAKTMSPDPVAVVELPHRVPYGFHAFFVTETSNEILIQ 464
            +V+VNPKP KG  +K ID +E LIVK  +D+S P H+L+GNF PV  ETPP  DLPVIG +PECLNGEF+RVGPN KF+PVAGYHW+      M+HGLRIKDGKA YVSRYV+TSRLKQEE FGGAKF KIGD+KGLFGLLMVNM +LR    VLD SYGNG                  AD    +KVL+DGDLQTLG+LDYDKRL H FTAHPK+DP+T EMFTFGY+ TPPY+TYRVISKDG M+DPVPIT++ PIMMHDFAITENYAI +DLP+ F+ KEMVKE KLI+ FD T KARFGVLPRYA+++L I+WFELPNC+IFHNANAW EE +EVVLITCR+ NPDLDM NG +KE LE+   ELYEMRFNMK+GLASQKKLS + VDFPRINE Y GRKSA  +IDAKTMS DPVAV+ELPHRVPYGFH  F++E   E L +
Sbjct:   65 IVVVNPKPQKGLAAKVIDSLEGLIVKFKHDSSRPLHYLSGNFGPVRHETPPTADLPVIGFLPECLNGEFIRVGPNSKFAPVAGYHWF--DGDGMLHGLRIKDGKAAYVSRYVKTSRLKQEEFFGGAKFTKIGDVKGLFGLLMVNMTLLRESFKVLDFSYGNG------------------AD---VLKVLKDGDLQTLGMLDYDKRLNHPFTAHPKIDPYTDEMFTFGYTQTPPYVTYRVISKDGFMHDPVPITMSAPIMMHDFAITENYAIFLDLPMCFRQKEMVKENKLIYAFDPTIKARFGVLPRYARDDLQIRWFELPNCYIFHNANAWEEEEEEVVLITCRVRNPDLDMFNGPLKETLEDQTTELYEMRFNMKTGLASQKKLSPACVDFPRINEHYMGRKSAVYVIDAKTMSADPVAVIELPHRVPYGFHGIFLSEEQLEELAK 490    
BLAST of DCAR_022385 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A835H631 ((Uncharacterized protein {ECO:0000313|EMBL:KAF9592759.1}))

HSP 1 Score: 763.066 bits (1969), Expect = 0.000e+0
Identity = 357/442 (80.77%), Postives = 398/442 (90.05%), Query Frame = 0
 
Query:   18 VVIVNPKPSKGFTSKAIDWIESLIVKLMYDASVPHHWLAGNFAPV-DETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGYHWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKKLSASAVDFPRINESYTGRKSAANIIDAKTMSPDPVAVVELPHRVPYGFHAFFVTETS 458
            +V ++PKP KG +S  +D +E LIVK MYDAS PHHWLAGNFAPV DETPP+ +L V G +PECLNGEFVRVGPNPKF+PVAGYHW+      M+H +RIKDGKATYVSRYV+TSRLKQEE  GGAKFMKIGDLKG FGL MVNMQ+LR KL VLD+SYG GT NTA+IYHHG+LLALSEADKPY ++VLEDGDLQTLG+LDYDKRLTHSFTAHPKVDPFTGEMFTFGYSH PPY TYRVISKDG+M+DPVPIT++ P+MMHDFAITENYA+ MDLPLYF+PKEMVKEKK IFTFD TKKARFG+LPRYAK+EL I+WFELPNCFIFHNANAWEE +E+VLITCRLE PDLDMVNG +K+KLENF NELYEMRFN+K+GLASQKKLSASAVDFPR+NESYTGRKS+ N+IDAKTMSPDPVAVVELP+RVPYGFHAFFVTE  
Sbjct:   18 LVKIDPKPQKGVSSWFLDLVEKLIVKFMYDASKPHHWLAGNFAPVIDETPPSHNLLVKGELPECLNGEFVRVGPNPKFTPVAGYHWF--DGDGMVHAIRIKDGKATYVSRYVKTSRLKQEEYVGGAKFMKIGDLKGFFGLFMVNMQILRAKLKVLDVSYGTGTGNTALIYHHGKLLALSEADKPYVLQVLEDGDLQTLGMLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHAPPYCTYRVISKDGIMHDPVPITVSAPVMMHDFAITENYAVFMDLPLYFRPKEMVKEKKFIFTFDETKKARFGILPRYAKDELQIRWFELPNCFIFHNANAWEEENEIVLITCRLEKPDLDMVNGPVKDKLENFGNELYEMRFNLKTGLASQKKLSASAVDFPRVNESYTGRKSSVNVIDAKTMSPDPVAVVELPNRVPYGFHAFFVTEVC 457    
BLAST of DCAR_022385 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A059BJ85 ((Uncharacterized protein {ECO:0000313|EMBL:KCW66099.1}))

HSP 1 Score: 753.436 bits (1944), Expect = 0.000e+0
Identity = 363/482 (75.31%), Postives = 399/482 (82.78%), Query Frame = 0
 
Query:   21 VNPKPSKGFTSKAIDWIESLIVKLMYDASVPHHWLAGNFAPV-DETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGYHWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKKLSASAVDFPRINESYTGR---------------------------------------------KSAANIIDAKTMSPDPVAVVELPHRVPYGFHAFFVTE 456
            VNP P KG TSK +D+IE LIVK MYD+S PH +LAGNFAPV DETPP  DLPV+G +P+CLNGEFVRVGPNPKF+PVAGYHW+      M+HG+RIK+GKATYVSRYVRTS+LKQEE +GGAKFMKIGDLKGLFGLLMVNMQMLR KL +LD+SYG GT NTA++YHHG+LLALSEADKPY +KVLEDGDLQTLG+LDYDKRL+HSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDG M+DPVPIT++DPIMMHDFAITENYAI MDLPLYF+PKEMVK+ KLIFTFD TKK+RFGVLPRYAKNEL I+WFELPNCFIFHNANAWEE DEVVL+TCRLEN DLDMV+G++KE LENF NELYEMRFNMK+GLASQKKLSA +VDFPRINE YTGR                                             KSA N+IDAK MS DPVAVVELPHRVPYGFHAFFVTE
Sbjct:   26 VNPTPKKGVTSKVVDYIEKLIVKFMYDSSRPHQYLAGNFAPVADETPPVTDLPVVGHLPDCLNGEFVRVGPNPKFAPVAGYHWF--DGDGMVHGMRIKNGKATYVSRYVRTSKLKQEEYYGGAKFMKIGDLKGLFGLLMVNMQMLRAKLKILDVSYGTGTGNTALVYHHGKLLALSEADKPYVLKVLEDGDLQTLGMLDYDKRLSHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGFMHDPVPITISDPIMMHDFAITENYAIFMDLPLYFRPKEMVKDNKLIFTFDKTKKSRFGVLPRYAKNELQIRWFELPNCFIFHNANAWEEEDEVVLVTCRLENLDLDMVSGTVKEHLENFGNELYEMRFNMKTGLASQKKLSAPSVDFPRINECYTGRYSFSVFDLGPGRFGSEAVFVPREPGITSEEDDGYLIFFVHDESTGKSAVNVIDAKNMSSDPVAVVELPHRVPYGFHAFFVTE 505    
BLAST of DCAR_022385 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A5J4ZQG1 ((Uncharacterized protein {ECO:0000313|EMBL:KAA8520239.1}))

HSP 1 Score: 750.74 bits (1937), Expect = 0.000e+0
Identity = 353/414 (85.27%), Postives = 386/414 (93.24%), Query Frame = 0
 
Query:    9 SEQVERIKD-VVIVNPKPSKGFTSKAIDWIESLIVKLMYDASVPHHWLAGNFAPVDETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGYHWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKKLSASAVDFPRINESYTGRKS 421
             ++ ER++D VV VNPKPSKG TSKAIDW+E LIVKLMYD+S PHH+L+GNFAPVDETPP +DL + G +P+CLNGEFVRVGPNPKF+PVAGYHW+      MIHG+RIKDGKATYVSRYVRTSRLKQEE FGG+KFMK+GDLKGLFGLLMVNMQMLR KL VLD+SYG GT NTA+IYHHG+LLALSEADKPYA+KVLEDGDLQTLG+LDYDKRL HSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVM+DPVPIT++DPIMMHDFAITENYAI MDLPLYF+PKEMVKEKKLIFTFD TKKARFGVLPRYAK+ELLIKWFELPNCFIFHNANAWEEGDEVVLITCRLE+PDLDMVNG +KEKLENF N+LYEMRFNMK+GLASQK+LSASAVDFPR+NESYTGRK 
Sbjct:    2 GKEEERMEDGVVAVNPKPSKGLTSKAIDWLEKLIVKLMYDSSQPHHYLSGNFAPVDETPPCKDLQIKGYLPDCLNGEFVRVGPNPKFAPVAGYHWF--DGDGMIHGIRIKDGKATYVSRYVRTSRLKQEEYFGGSKFMKVGDLKGLFGLLMVNMQMLRAKLKVLDVSYGTGTGNTALIYHHGKLLALSEADKPYALKVLEDGDLQTLGMLDYDKRLAHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMHDPVPITISDPIMMHDFAITENYAIFMDLPLYFRPKEMVKEKKLIFTFDPTKKARFGVLPRYAKDELLIKWFELPNCFIFHNANAWEEGDEVVLITCRLEDPDLDMVNGVVKEKLENFKNQLYEMRFNMKTGLASQKELSASAVDFPRVNESYTGRKQ 413    

HSP 2 Score: 72.0182 bits (175), Expect = 8.770e-11
Identity = 32/37 (86.49%), Postives = 34/37 (91.89%), Query Frame = 0
 
Query:  420 KSAANIIDAKTMSPDPVAVVELPHRVPYGFHAFFVTE 456
            KSA  +IDAKTMS DPVA+VELPHRVPYGFHAFFVTE
Sbjct:  496 KSAVTVIDAKTMSADPVAIVELPHRVPYGFHAFFVTE 532    
BLAST of DCAR_022385 vs. ExPASy Swiss-Prot and TrEMBL without DCAR
Match: A0A7J6DKT2 ((Uncharacterized protein {ECO:0000313|EMBL:KAF4346728.1}))

HSP 1 Score: 746.503 bits (1926), Expect = 0.000e+0
Identity = 361/454 (79.52%), Postives = 398/454 (87.67%), Query Frame = 0
 
Query:   19 VIVNPKPSKGFTSKAIDWIESLIVKLMYDASVPHHWLAGNFAPV-DETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGYHWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFHN-----------ANAWEEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKKLSASAVDFPRINESYTGRKSAANIIDAKTMSPDPVAVVELPHRVPYGFHAFFVTETSNE 460
            V VNPKP+KG +SK +D++ESLIVK MYD+S P H+L+GNFAP+ DETPP +DLPVIG +PECLNGEFVRVGPNPKFSPVAGYHW+      MIHGLRIKDGKATYVSRY                   IGDLKG FGLLMVNMQMLR KL VLD+SYGNGTANTA+IYHHG+LLALSEADKPY +KVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGY+HTPPYITYRVISKDG M++PVPIT++DPIMMHDFAITENYAI MDLPL FKPKEMVKEKKLIF+FDATKKARFGVLPRYAK+ELLIKWFELPNCFIFHN           +NAWEE DEVVLITCRLENPDLDMV+G++KEKLENF+NELYEMRFNMK+GLA+QK+LSASAVDFPR+NESYTGRKSA ++IDAKTMS DPVAVV+LPHRVPYGFHAFFVTE +N+
Sbjct:   21 VSVNPKPNKGLSSKLVDFLESLIVKFMYDSSQPQHYLSGNFAPIRDETPPIKDLPVIGYLPECLNGEFVRVGPNPKFSPVAGYHWF--DGDGMIHGLRIKDGKATYVSRY-------------------IGDLKGFFGLLMVNMQMLRAKLKVLDLSYGNGTANTALIYHHGKLLALSEADKPYVLKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYAHTPPYITYRVISKDGFMHEPVPITVSDPIMMHDFAITENYAIFMDLPLIFKPKEMVKEKKLIFSFDATKKARFGVLPRYAKDELLIKWFELPNCFIFHNGAHSGLPTFYVSNAWEEEDEVVLITCRLENPDLDMVSGTVKEKLENFSNELYEMRFNMKTGLATQKQLSASAVDFPRVNESYTGRKSAVHVIDAKTMSSDPVAVVDLPHRVPYGFHAFFVTENNNQ 453    

HSP 2 Score: 626.32 bits (1614), Expect = 0.000e+0
Identity = 300/415 (72.29%), Postives = 350/415 (84.34%), Query Frame = 0
 
Query:   13 ERIKDVVIVNPKPSKGFTSKAIDWIESLIVKLMY-----DASVPHHWLAGNFAP-VDETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGYHWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKKLSASAVDFPRINESYTGRKS 421
            +R K++  VNPKP+ G  SK IDW E L+VKLMY      +S   H+L+GNF P   ETPP +DL V+G +PECLNGEFV+VGPNPKF+PV+GYHW+      M+HG+RIKDGKATYVSR+V+TSRLKQEE FGGAK +K GDLKG+FGLLM+++ MLR K  VLD+SYG GTANTA++YHHG+LLAL E DKPY +KVLEDGDLQTLG+LDYDKRLTH FTAHPK+DP TGEMFTFGYS TPPYITYRVISK+G M+DPVPIT+ +PIM+HDFAITENYAI MDLPL F PK+MV + KLI +FD TKKARFGVLPRYAK+ELLIKWFELPNC IFHNANAWEE DEVVLIT R+EN DLDM+NG++ +K  +  NELYEMRFNM++GLASQ+KLSA AVDFPR+NESYTGRK 
Sbjct: 1594 KREKEISEVNPKPNNGLASKLIDWFEKLVVKLMYYNNSSSSSSALHFLSGNFTPNRHETPPTKDLHVLGYLPECLNGEFVKVGPNPKFNPVSGYHWF--DGDGMVHGVRIKDGKATYVSRFVKTSRLKQEEYFGGAKILKFGDLKGIFGLLMLSLYMLRQKFKVLDLSYGFGTANTALVYHHGKLLALHEGDKPYVLKVLEDGDLQTLGMLDYDKRLTHPFTAHPKIDPCTGEMFTFGYSQTPPYITYRVISKEGFMHDPVPITIPNPIMIHDFAITENYAIFMDLPLIFSPKDMVTKNKLIISFDTTKKARFGVLPRYAKDELLIKWFELPNCNIFHNANAWEEEDEVVLITSRIENLDLDMLNGTINKKPYSIPNELYEMRFNMETGLASQRKLSAPAVDFPRVNESYTGRKQ 2006    

HSP 3 Score: 551.592 bits (1420), Expect = 1.376e-176
Identity = 276/461 (59.87%), Postives = 326/461 (70.72%), Query Frame = 0
 
Query:   15 IKDVVIVNPKPSKGFTSKAIDWIESLIVKLMYDASVPHHWLAGNFAPVDETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGYHWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMS---------------YGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKKLSASAVDFPRINESYTGRKSAANIIDAKTMSPDPVAVVELPHRVPYGFHAFFVTETSNE 460
            +K +V VNPKP+KG TSK IDW E++IVKLMYD+S   H+L+GNF  V ETPP +DLPV+G +PECLNGEFVRVG N KF+PVAGYHW+      M+HG+RIKDGKATYVSRYV+TSRLKQEE FGG+KF+K+GDLKGLFGL M ++Q LR K    D+                Y +  ANTA++YHHG+LL L EADKPY +KVLEDGDLQTLG+LDYDKRL H+FTAHPKVDPFTGEMFTFGYS +PPY+TYRVISKDG M+D                                 +EMV E KLI +FDATKKARFGVLPRYAK+ELLIKWFELP+C+IFHNANAWEE DEVVLITCRL+N D++M++G +K+KL +F  ELYE                                KS  ++IDAKTMS DP+AV+ LP RVPYGFHAFFVTE SN+
Sbjct:  495 VKGIVTVNPKPNKGLTSKLIDWFENVIVKLMYDSSQHQHYLSGNFTSVPETPPIKDLPVMGYLPECLNGEFVRVGSNSKFAPVAGYHWF--DGDGMVHGVRIKDGKATYVSRYVKTSRLKQEEYFGGSKFLKVGDLKGLFGLFMFSIQTLRDKFKEADIYSDTFLPLVMEGLIVFYHSLPANTALVYHHGKLLVLHEADKPYVLKVLEDGDLQTLGMLDYDKRLAHTFTAHPKVDPFTGEMFTFGYSLSPPYVTYRVISKDGFMHD---------------------------------QEMVMENKLILSFDATKKARFGVLPRYAKDELLIKWFELPSCYIFHNANAWEEEDEVVLITCRLDNIDMNMISGEIKKKLNHFRTELYE--------------------------------KSEVHVIDAKTMSADPIAVIHLPCRVPYGFHAFFVTEVSNQ 888    

HSP 4 Score: 531.561 bits (1368), Expect = 2.881e-169
Identity = 269/450 (59.78%), Postives = 324/450 (72.00%), Query Frame = 0
 
Query:   18 VVIVNPKPSKGFTSKAIDWIESLIVKLMYDASVPHHWLAGNFAPV-DETPPARDLPVIGSIPECLNGEFVRVGPNPKFSPVAGYHWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGL--------FGLLMVNMQMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFHNANAWEE--GDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKKLSASAVDFPRINESYTGRKSAANIIDAKTMSPDPVAVVELPHRVPYGFHAFFVTE 456
            VV VNP P+KGF+S  ++W E+LIV+ MY++S  HH+L GN+APV  ETPP  +LP++G +P+CLNGEFVRVGPNPKF+P+AG+HW+      MIHGLRIKDGKATYVSRYVRTSR+KQEE F  AKF K  + K          FGLLMV                     N  M+    ++L LS  +                                      TGEMF+FGY+HTPPYITYRVISKDG M++PVPIT+ +PIMMHDFAIT+NYAI MDLP+ F+PKEMVKEKK+IF+FD TKKARFGVLPRYAK+ELLIKWFELPNCFIFHNANAWEE   DEVV+ITCR+ENPDLDM +G +KEK  + N  LYEMRFNMK+G+ASQ++LSASAVDFP++NESY GRKS  ++IDAKTMS DP+AV++LP RVPYGFHA F++E
Sbjct: 1177 VVCVNPNPNKGFSSMLLNWFENLIVRFMYNSSSSHHFLTGNYAPVLRETPPVDNLPLLGYLPKCLNGEFVRVGPNPKFAPIAGFHWF--DGDGMIHGLRIKDGKATYVSRYVRTSRIKQEEYFERAKFTKKREWKKYWNWRPKRNFGLLMV---------------------NIQMLRAKLKVLDLSYGNG-------------------------------------TGEMFSFGYAHTPPYITYRVISKDGFMHEPVPITVPNPIMMHDFAITQNYAIFMDLPMIFRPKEMVKEKKMIFSFDTTKKARFGVLPRYAKDELLIKWFELPNCFIFHNANAWEEEDDDEVVMITCRVENPDLDMYSGEVKEKF-SVNTSLYEMRFNMKTGVASQRQLSASAVDFPKVNESYIGRKSMVHVIDAKTMSADPIAVIDLPCRVPYGFHALFISE 1565    

HSP 5 Score: 270.396 bits (690), Expect = 2.834e-77
Identity = 164/309 (53.07%), Postives = 190/309 (61.49%), Query Frame = 0
 
Query:   78 PECLNGEFVRVGPNPKFSPVAGYHWYVLALRSMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNMQMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFAITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCFIFH-NANAWEEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNEL 385
            P+CL G   R        P             MIHGLRIK+GKATY SRYVRTSRLKQEE FGG KFMKIGD+ G+FGLL+V +Q+LR  L VLD+SYG GT                             G++ T G       +TH                        PYITYRVISKDG M+DP+PIT+ +PIMMHDFAIT+NYAI MDLPL F+P+EM+K K  I +FDATKKARFGVLPRYAK+ELLIKWFELPNCFI H  A+  EE DEVV+ITCR+EN DL M  G +K+KL N N  L
Sbjct:  891 PKCLFGTTTRTSRALNCEP-----------NLMIHGLRIKNGKATYASRYVRTSRLKQEEYFGGPKFMKIGDMNGVFGLLVVIIQILRAILKVLDLSYGLGT-----------------------------GEMFTFGF----SLITH------------------------PYITYRVISKDGFMHDPIPITIPNPIMMHDFAITQNYAIFMDLPLIFRPEEMMKGKNFIISFDATKKARFGVLPRYAKDELLIKWFELPNCFISHIGAHFSEEDDEVVMITCRIENLDLSMYGGDIKQKL-NINASL 1130    

HSP 6 Score: 67.0106 bits (162), Expect = 5.195e-9
Identity = 35/63 (55.56%), Postives = 47/63 (74.60%), Query Frame = 0
 
Query:  394 SGLASQKKLSASAVDFPRINESYTGRKSAANIIDAKTMSPDPVAVVELPHRVPYGFHAFFVTE 456
            +G++SQ++     + F  +++  +G KS  ++IDAKTMS DPVAVVELP RVPYGFHA FVTE
Sbjct: 2067 NGVSSQEEDDGYLIFF--VHDENSG-KSTVHVIDAKTMSDDPVAVVELPSRVPYGFHALFVTE 2126    
Match NameStatsDescription
Q2VEW7E-Value: 1.847e-13, PID: 97.86(Putative carotenoid cleavage dioxygenase {ECO:000... [more]
A0A7J6F6N7E-Value: 8.598e-94, PID: 83.33(Uncharacterized protein {ECO:0000313|EMBL:KAF4365... [more]
A0A660KPA2E-Value: 0.000e+0, PID: 83.56(Uncharacterized protein {ECO:0000313|EMBL:KAE8038... [more]
A0A2H5NBG6E-Value: 0.000e+0, PID: 82.88(Uncharacterized protein {ECO:0000313|EMBL:GAY3700... [more]
A0A4P1RC87E-Value: 0.000e+0, PID: 80.89(Uncharacterized protein {ECO:0000313|EMBL:OIW0798... [more]
A0A2H5N9V9E-Value: 0.000e+0, PID: 79.27(Uncharacterized protein {ECO:0000313|EMBL:GAY3700... [more]
A0A835H631E-Value: 0.000e+0, PID: 80.77(Uncharacterized protein {ECO:0000313|EMBL:KAF9592... [more]
A0A059BJ85E-Value: 0.000e+0, PID: 75.31(Uncharacterized protein {ECO:0000313|EMBL:KCW6609... [more]
A0A5J4ZQG1E-Value: 8.770e-11, PID: 85.27(Uncharacterized protein {ECO:0000313|EMBL:KAA8520... [more]
A0A7J6DKT2E-Value: 1.376e-176, PID: 79.52(Uncharacterized protein {ECO:0000313|EMBL:KAF4346... [more]

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Analysis: 
NameDescription

An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4.

There are a few different naming schemes for this assembly. First there is the
Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below.
These sequences can be viewed in JBrowse here.

Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0

LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926.

Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence.

RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records.

The NCBI Sequence report lists the correspondences between the various naming methods

Link to the LNRQ01000000.1 master record at NCBI

Raw Reads: Link to SRA accessions used for the genome assembly

This genome is available in the CarrotOmics Blast Search

This analysis is a blastp search of all of the DCAR V1.0 Gene Prediction polypeptide sequences against combined ExPASy SwissProt and TrEMBL databases from Nov. 17, 2021. Prior to performing the blast search, the database was filtered to remove organisms not in the Viridiplantae, and also filtered to remove these same DCAR gene predictions.

For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins.

Data from this analysis can be viewed in JBrowse here.

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