Relationships |
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The mRNA, DCAR_014610, is a part of gene, DCAR_014610. |
The genetic_marker, MK001006 GSSR-007, is located in gene, DCAR_014610. |
The genetic_marker, MK017939 4_20498674, is located in gene, DCAR_014610. |
The genetic_marker, MK017937 4_20498827, is located in gene, DCAR_014610. |
The genetic_marker, MK017938 4_20498875, is located in gene, DCAR_014610. |
Name | Description |
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An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |
Transcript Name | Identifier | Type | Location |
---|---|---|---|
DCAR_014610 | DCAR_014610.mRNA | mRNA | DCARv2_Chr4:20503388..20506220 |
TCTGAATTTAAGCATACGAATAATTATCATCCCTATTTTCACTAAACTTT
AATCCTAAATCTGTCCATGGATGCTATTTATCTCCCCCGGCGTGACAGAT
TTTCGATTCGATGCTATTTATCTCACTTTTTTTACTGTACGCAGAGGATT
GAACTTTGGCAAGATGTTGTGTCATTTCATTTGTGCCTGTGTTTAATTGG
AAAAAACAAATATTAAAAAAAAAGCGCAACAAGTACCCGTTCTTCTTTTC
TAGCTATCTCTCTACGCAAGCAAGGCACCTTAAAAACACACATCTAAAAC
ACACGCACACGCGCACACACACTCTGTATCCCCCGGAGCTTCCTCCTCAA
CTTTGGCAATCCATCAACTTCTGTACTCATACATACATCATGAGACTAGT
CGTGCCTTTGCAGGGACTCATAGTTCACGGAAGAGGCGGTTTAGTATTAG
GCTCACTCCTACCTTGTGCTCTCTTCTACTTCCTGCAATTTCACCTCAAA
CGACACCGTTCTCCTGCTAATTCCACTCCATCCTCTTCATCTTCGTCGCC
AATCCGCGCGCAGTTGTCATCTAGGGCCAGCCCAATCGCTAAGCCCAATG
AATCGCCGTATTACATTGGAATGGACATGGTGCGCTCCGATCCGTATCAC
AGTCTCCGCAGTCCAGATGGTATCATTGATCTCGGGATTGCTGAAAATAG
GGTATCGATTTTTATTTGTTTTTTTTTATATGTTTATATAATTGATCATG
TAGCACTTTCCTAGTATGCTGTATTCGTTGTTGAAATGTCCATGTCATAT
TCTACACCCGAACAATTCGTGCACCCAAACTCGAAAGTGTTGCATTAGTA
GGTTTTTTGCTTAGTTTCGAATCGAGATTTTTGGAATGTAGGTGATGTTT
GGTATTATTTTCTGTTTTTAGTTTTCGAATTTAAAGGTGTTTGGGAACTG
TTTGTCAAAACAAAGTTTGGAAAATAGGATACACCATTTTACTTGATTAT
GTAGAGTAATTGAATCTTCATGTTTAGTCAGTAGGAAGGTTGAAGATATC
GAGAGAGTTTTAGATTTAGATAACTATTTTATAGTTCCCCAAAAAAAAAC
TTGTTTTCTATTTTTTAAGGAACAAAACCACATTTGTTTTGTGAAAAACA
ATTCTATAGCCAAACATGCACTCATGTTGTGTTTGGTTGGCGAGGATGGA
ATGGAATGAATAAAAACAAATGAAAATAGAAGAGGTAGGAGTGAAGTTTT
GAAAAAAAATGAGTGAGTGCAAAATTGTTATGATGAGATTTGAGAAGAGA
TCGAGTATAGGAGGGAATGAAGCATTCTTTCTCAAACTGATGGTGTGGTA
TCTAGTATAGGGTGTAAAGAATGGAATGGAGGAAGGAATGGAATTTATAA
ACAATTGACCAAAACATAGTTCAAATTTCATTCCATTCCTTCCAAATTAA
TTCACGCCAACCAAATACAACATTTCTGTTATAAGATCATGGTATTACAT
AATGAATCTTTACATGAACTTTTACATTTCCTGTAAGATATTTCAAATTA
AAACACTAGATTTTCGTGTCATTACAAATCGTTGGGTCTCTCATTGTAGT
TAAATTTTACAGGCAAATATCCTATTAAGGTCTAAACCATGTTTAAGACA
TTAATTTTGGTAGTTATGCTATGTTTTGATCTTAATTATTTGATCGTGTT
ATTCGGTTGTAGTTGTCGTTGGATTTGATTGAGACTTGGCTTCTTCAAAA
TTCAAATGATTCCTTACTGCAAGGAGGCTTGACAATTGGTGGTCTTGCTA
CCTACCAGCCATTTGATGGATTGATGGATATGAAAGTGGTAATTGTACAA
ATAAATCATTGTTGATTATATTTCATAGATCAACCATATGATTTCTCAAT
TTTGATGATAAAATCTAATGTAATTTATGTTCACTTCATTTGACCGATTA
TCAGATGTGGTGCCAAATAATAGTGTTGATTCATTGGTTCATAATACTCT
TTACATTGTAAAAGTTAACCTGAGGTTATATAAGTTGTAACTAAGGTTTA
TATTATTTTGATTCTGTTGTGTAGGCAATGTCAGGTTTTGTTTCTGAGGT
GATTGGAGGAACAATATCATTAAACCCATCTCAACTGGTGTTAACATCTG
GTGCAACCCCTGCTGTTGAAATACTCTGCTTCTGTTTAGCTGACCCCGGG
GATGCATTTCTCATTCCAGCACCATATTATCCTGGGTAATCTTCCTTATA
TGAAACATATATATCAATCTTTTCCTTTTGTCACTTCGTGTGAGAATATT
CAGTTTTTTGTTTTTTTGAAATAATAATGAGCTGTAATATGGATGCCATT
TCTCAGGTTTGACAGGGATGTATTATATCGGACAGGAGTGGAGCTGATTC
CTGTTCATTGTGGTAGCTCTGATGGCTTCATGTTGAGTAAAAATACTCTT
GAACAAACATTTAATCAGGCAAGAAAACGAGGGAAAAGAATCCGTGGGGT
TCTGATATCAAACCCTTCGAACCCCGTTGGTAATATACTAAACAGAGAGT
TGCTCTACAATCTCTTAACATTTGCCGAAGAGAAGAATATTCATATTATA
TCAGATGAGGTATTTGCTGGGTCTGTTTATGGAAATGAAGAGTTTGTAAG
CATGGCCGAAATTCTGCAGTCGGAAGATTTTGAGAAGGAAAGGGTCCACA
TAATATATGGGCTGTCCAAAGACCTGTCTCTTCCTGGATACAGAGTAGGG
GCTATTTATTCCTGCAATGAAAATGTTTTGGCTGCTTCTAAAAAGTTGAC
AAGATTTTGTTCCATCTCAACTCCAACTCAGCAGCTACTAATTTCAATGC
TTTCGGACAAAAGGTTCATTCAACAATATATAAAGATCAATAGAGAGAGG
CTGACAAAAATGTATGATCTGTTTGTGGAAGGATTAAAACAATTGGGGAT
TGAGTGTGCAAAGAGCAGTGGAGGATTGTACTGTTGGGTCAATATGAGTG
GATTAATCAGTTCACGTGATGAGAAAGGAGAACTTGCCCTTTGGCACAAA
CTCCTAAATATTGCTAAGATAAATATTACTCCAGGATCAGCTTGCCACTG
CATTGAGCCTGGATGGTTCAGGTGTTGTTTTACTACCTTACAAGAAAATG
ATATTCCTATTGTCATGGGCAGGATTCAGAAAATTACTCCAATAGGCACA
TCTCAAACTGGAATCCCATGATTATTGTATTATAGGATTCTGTTCATATC
TATAAAGATCATATAAACATGATAGGTATCTCAAGGTTTGACACATATTC
AGAGCATAATTGCATTGTGAAGGAACTCATATTTTAGACATGTGATATAT
GAAATGAAATGAAATTAATGTGGACAGCAGTCTGAAGGAGACTTTCAACA
AAAGTGGCATTAACTAAAATTGCAGAGGGACTTTCTTAACTTGGCTTCAG
TTGCAGGTGCTTCTTCCACTTGCCCTGTATTCTTTTGATCTTGTTGACAC
TTCCGACCATGCAACAACCGAGGGCACTTTTCTTCATAAATTCCTGCAAG
TAAGATATGCAGGCAAGAGTATATCCTTGTAAATATTGTAGAAAATAAGC
AAAAGGTACCTTTGAATTATCTGTTTTATGTCGTTATTATGTTCGAACTT
GATGAAATTAAGATTCAGTTTTTGTGATGTTGAAGAGACTCATTGATGCA
TTATGCTTTTGGTATTGGTATAAAGAGAATAATTTGTAAAAAAGTGAGAT
TATTCATAGCAAAGAAGTTCAAATTGTGAAATTACCATGGAGTGATTCTG
GTCTCGCATTAAAATAGCTTTCCTGTGGTAGCTTCTTTTGCGACTGATCA
ATATTTGCTCCCCAAGAGTTAACGGCCTCTTCACTACAAGTTTTTGGTCA
AGTTCATAATTCATGTCTGAAACCCTGCTTGGCAGTGTCTGTGGGGTAGT
ATGTGTACCCTTCTCGCATCCAACCTTTTCCTGGAAACTTGACTTGCGCA
ATAACAATGGAAAGAGACGTTCTAAAATTGGAGTGTCGTCTAGGCTTGTA
TTACCTGTTGCATAGCTGCTTGTACTGCTGTCACTCTCATATATAGGAGA
AAAGGTTGCTTGTAGCTGAAGATTGTTCCCATTCATCAGACCATTTTCTA
CCACTGGAACAGCATTAGATATTGCCAGAGTTGGTATTAGTCGATTGGCC
AAAAGCGATGGAGCTAAGCTGATCAACTCCACGTCTTCAAAACCCCATGC
ATCAAGGTATGAATCAATTATTTCATCTTTTTCATCATCACCATCACATC
CATCATCATCATCTGCATTATACGAAATATTCTGGGAGTACACTGTAGGT
CTAGGTGATTGAGAATCACGTAGCATAGGTGGTGGTAAGTGCACTACTGG
ACCTGCATCTGGCATCTCATGTGGGAAGCAATGTAGAGGTTTTCCTGGCA
TTTCTTCCCACTGGATTAATGAATCCAGGGCGGTCAGCTTGACTGGAGGC
AAAGCAGTTGGACCAACTGGTGCAATATGCTTCCAGTCCGTTTTAGGGAG
ACCTGGTCTTACTTCCCAAATAAATGGAACTGAAGCTGGCTGTTTTATTC
GCTGCCGGGTTGAACTTTGGGACAAAAATTGGTTTGCTACCATTATGCCT
AAAGCGAAAATTTGATCTAATAGCTTGTGCTGCCCAAACATATTTGTGCT
GGCCATATATACAAACCTGTAGGTAATACCGGGTTAGAGAACTTGGAATC
TATTAGTTACGGATACCAACTGCACTAATATTAAGTAAACGACATTAGGC
AGAGCTGCTCTTATAAACTACTGCGCTTTTGTGTTGGACACATAAAAGGG
TGGTAATGTGGTATTAGGTTCTTGTAACTACTGTTGAACAATCTGCGTTA
AACCAAACAACAAAACTATGCTGTTTGTGTGTAATGAAAAAAGCGTGACA
AAGCAAAAGATAAGGAGCAAGTATAACAATAACAAAACCCGAGTCAGCGC
TGCCTCACCGTATGCGTAGAACGATCGCTTCCCAGGATAAAACAGATTTC
GATGGCACCACCAGCAAGCCAGAAATCGAGCAAGAATAATTAACCATAAC
ATTCTATAGGAATATATACAAGTGTGGAGAGACTAGTGTGTTATTCTTGT
CTATAATTAAATAACATCAAATATGGAATAAATTATGGAATCGGTGTAAA
ACTTGTTAACTACTCTATTCCATAAATAATCAGAAATAAAATCAGATTAA
AATTTGCAAGCTACTATATTCCATATATAATCTGAAACATGATCAGATTA
ACGCATGTTATAAATTTGAGCCCAATTTCATTAAAAAATTATAATTTCCA
CTAAAATTATAATTTCCACTTTTATGGAATATCTTTGAATCATCGTATCA
CCTTAGTCATAATTACTCGATATGACTTGCCAATATGAACTAAAACTCAT
ATTTTCCAACAACTACTCCCCCATCTGCCGATAGCCAGTTTTAACATGAA
TCGCGATATATATCAAGATATCATTAGCAAGTTACATGAACCTTTTCAAA
ATTTTATTTCTGAACAAGAAGCAATCATTAGGCATATAGAAAAGTACACA
TCGTTATTTCATACTGTAGTAAAAAAGGTTCTCTGGAAATCTGTCTAAAC
TCAGGAAGTTTTGGTTAACATGTTTATCTCATTATCTGCTATACATCTGT
TACCCCATGTAGGTTCTTCTTCTTCATAAGAAATCAGTATAATAAAATAT
TAGTGCAGATAAAAAGAAAAAGAGTTCTTATTGGAAATGCTAATTTGAAA
TCCTAATATACAGAGTAATATATATTACAAAAGGTTGCCAAACAAAATGT
CATTACAGTGGTTGATAATATGGCGCATATTAGGCTCAAATTTGTGGGCA
AGTTGAAGTCTGTTGAAGGTCCTCCAACTTGCATCACCACACATTTGACC
TTAATTTGTTGGGAGGCAGCAAAGGCGATTGCTCTGGCCCCCAAACAGCG
GCAGTGGCCATTTATTGGGCTTTATGCAAGCTGTTTTACTTCGCCCTACA
CACCGTTCTTCTACTATCTGCAAGTAATGTTTGATATATCTGCCAGCTAG
CTAGCCCTGCTAAGCAGCTTCTTAAGGTTGAAAGGTCGTTAGCTAAGAGG
GGTTTTAAATAGCCTGAGCATAATTATTCGAAACCGATCCGGTCCATCTA
ATATAAATCGTCCAAACTGAAATTTTCGGGTTTTTAAGAGTATGTTTCAT
TATTAGATACCGAAATTTTTTGTTTAAGTTGGATTTTCACCTTGACTGTA
GATAACTGAAACGTCTACATATATTTTATAAATAATATATTTATAGTTTA
GGTTAAATATTTAAGTGCCGTCCATTTTCTCTTGTTGTTTATTCATTTGA
AAATCATTTTCTATTCTTATCTACATCCTTATGCATCTGGTTAATAATTA
ATAATTTTTAATTAAATGAGTTATTTTTCTTCTTAACAACAAAAAAAGTA
ACGCATAAAACTATTATTTATTGAGAGTTATACAAATGTCATCTTTATTT
TCTAAGTGTTTTGTTGAGAGTCAGATAAAAGTAACACATAATTTTTATTT
TAGCTCAGTAAGAAAATATAATTAAAAAAAATTCGCATTAAGCCGAAATT
GAACTAAATCAATCCGTTAAGAATCGATTCGATTTGGATTGATTTTTTAT
AACATTCGGAGTTTGAGTTCAATTTCTAGATGTTCAAAATCGAAATTTTC
AATTTGGTTTGATTTAGACTTTAAAACTGTCCAAACTGAATTATGCTAAC
CCTGTTATTAAAAGTAGAAAATTTGTTCCCTGTTCTTAAGATTAGGAAAT
CTGTTTTTAAATTAAGCCGGATCCTTGCTAGATGATCAATTTATATATCT
TCCCACTTATTATCAAACTGTAGCAGCATATGTAGAGAACCAACTGAATC
AAATTCAGTGTGGAGAACAACAAATTACCAGATTACAAAAATAATATGTT
CACATGGTATGTATATGAACGAAAATGTTCTATTGAATATTTGATTCAAT
CATGTCTTATATATAAAATATAAACATTATATAATTACAACATACAGATC
AGATTTGAAGGCAAAACAAAACCTCGCAGATCTGATCCTATGACTTCAAT
CTATCAAATAACACTAAGAACAAGATGATGGTATCAACTTATAACCATCA
GAACTAAAAAAAAAACCAAGTGAAAAACAATACCAACGGGATTAAAACAT
GGCGAAGAAGAGCACTTGAATAGCCCAAGTGGTGGGTTTATCCTCTGTTG
TCCCAAGACACTTTCGACTCCGGCTCATCCGCTCAAAATTAGCTAGCACA
ATCATGTAATTTTACGAATCTATTAAAGTCATCACAATGTAATACGGCCT
TGGCGTTCATCTCTCTTTCAAATCTCCATATGTAATTCAGACCTACCAGC
AACTCAGTTATGGCATCTTCAGTCTTCTCCTGGTTGGCAAAATAGAGTGT
TTGCAACAAAAGAACATTCGTCTTCGGGGTCATAGTTTGCTGCTCGAAGC
TCCTTTCACTTTGCCACTTTATCATGTTATGCGCCAAGGGTGACAACCAG
CCTAGTATCTTCCCTAAAGCATCTTTCCATTCTCCAGCAAGAACTGGATC
AGTAGCCGCGAATCCTACCCCTTTTAGTCTTCCTCGGAGTGACCACCTAA
TGCTTTGTGGCAGCATCGCGTAGAGATCATCCCTTGCATCAAATCCAACC
AAGTGTGGTGACCTTATCATCTTCTCCATCACAATGATCAAATTTGAGTA
ATGTAGAGCTAGAGCAGCCGCGCCTAGTGTACCTGTCGGTGGTTTTATTA
TCTTAGAGTTTAATTCAAATAATCCTGGTGATTTTTCTTGTTGCTTCGTG
TTCTTGATCATGATCAATGGTCCAGAAACCATGCTGACAGAATTATCAAA
ATGGTTTTCAGATGGATAGACAGCTGCGGAAGCAGAGAGGCTTCGAGGCA
GAGAAGTGACTGGAATTGTATTTGCATGGCCTGTTCCAAAAACAAGCTTG
ATCCGTGCAAGAACCGTGAACACTGATCTTGCGAGCAGGATTGTAACTTT
ATCAAAATTCCGATTCCATAAAGACTTCTCCTTCTTATACTTGACCTCCT
GTCTCTGCCAAGCTAATTTCTGCTGTTGATCAATGATCTTAAGCTGCTTG
ATTGACGCCTCGTGTTCCTTGCACGACTTCAACGATTTCTTCACAACATT
TTCCACCGCAGAAAGCTCCTCCAGCTCTTTGTAAAGCGTCGCTGTTGTTT
TGACATACTTGTCCATTTTCCTGATTTTGGCTTCCATATCTCTCCAGCTC
ATGATCCAGTGACAAGGATCACACCCTGTTCTAGCGAACTCATCAAACAA
GCGATCAAACGACTGAAGATTCAAGTCATGGCACCGCTTGCTCATCACCG
ACAAGGACTTGACTAGTAGTCCAAGATTCTCCACCATCTCCGCGCAAGCA
AGGCTCAGAAGAAAACATTCATCACTGGACACAATCTTGTGAACGCCTTC
GAGTGAAACAGATTCTGTTCTAAGATGAGCTATATTCTTATCAGACAGGG
ATCGCCATAGGTGTATGAGTTTGGACATGAGGCTTGTGATCTCCAAGGCC
AAGACTCCGACATTCGACGTTTTCTTAACCATATGCTTGGTGTTTGCAGG
TGTGGAATTGAGAGTGCTTGATACCCTCTTCTTCACCTTGAGTAGCCATG
CTTCGAGTGCCATGGACTTATTCTTTACAGAGCAAATGATGAAGAATGAG
GTGGAGGAGTAGTTAAAGAAAGATAAGGTGTGTGGAAGATAATAATGTGG
GGGTGATGGCACTGGGAACAAGTTTGAATGGGGCTTGAAAAAGAGGTGCA
GGGAGTTTGTAATAATTCAAGTGCGGAGAAAGGAGTGGTGTTTGGGGCTC
GTGGGGTGTTTGAAAGCATTGCATACAATAGACTAATGAGAAATACACCA
ATGACAGAGAAACTTGTTTGTTCAACATAAATTATATAACTTGTGGCCAC
ATGACATGGAGGTGCAAACGGAAAGAAGAGTGAACAAACAAATTAACGCA
TTTACGTGCAAAATGCGATAATGGTCCGATCTCGTCTCAGATCCCGACTG
AACTGAAAAAATTAGTAAAATTCATCATTTAACTAACGTCTCATCATTTT
CTTCAATAAAAGTTTATTACTGGATACCCTGTCTAGGATTATTTCCTAGA
AATCTGCCGACTAGCTCCATAGGAAACACAGGTAGGTAAATTGGACTAAT
CGAGCGATAAGACCCGGTGGTTGAGAACTTTTATGCGATACGCACGGATT
GGAACCCGATCTCCTTTCTTCAAATCATCTCACTGTCTATTCAACTCGGG
TGAACGTCCGGGATCTCATCATTTTAAAAGATGATAAAAGAAAAATTGTT
GAGCATTGCCTTCGATTTGAATATTAAATATTTTAACTTGGAGTGTTTCA
GGATCATGATTCCGCCGTAGTGTAGATATAGGGGTCGTTTGGGTTAGCTT
AAAAAAAGTGATTTCTTGCTTATATTAAAGAAGTGGAGGAGAGAAGTGAA
AAGTAAAGAAGTTAATAAAGTGTTTGGAAAACAAGCAGAAGCTGTGAGAG
AAGCTAGCATTCTTATCTTTTTAAAAATGCTTCTACTTCTTTATACAAAC
AGATCAAGAGAAGTAGAAGTCAGAAGCATTCTGCTTCTCTCAACCAAACA
GGGCCATATATAGAGAATTACAGATTCTTTAAAGCTTGGGGTTGGAATTG
TTTTCAAGTTGCGAAATGCTAGGGTTGGAGTGGTCAATTTGGATTAATAA
AATGTGGTGGTTTAATTTCCTTATCGTATCTTTGAGTGGGCAAAAGGTTC
CACTCGGATTTGTTTAGATACTGCATGATAAAGATACCTCCTGTGTTCAA
TTGTTAGTGCTGAAATTTCTTCTTCGTACACTCACATCAAAGCAAGTTGG
TGTTCTTTCTTCATTCCTCCATTAGATCAGCGGAATAGGTTGTGCATATG
AAATTAAAATCAGATGTTAATCGAAATTGTCACTCTTTTAGGGTTTATCA
AGTCATTTACGAGTTCGAATATCAATATTTGTATTTCAAATATCTTATGT
CTTCATTGAGAGTTGAAGCATAGAATGCTTCCTCAGGCTAACCGTATACG
TACGCATGAAATCAAACTTATGCTCCCTTTTCGTCAAAAACCCAATCATT
TTGCTTCTGACCCTAAGTTTATTCTTTTACTCATTTTCTAAACTCCATCC
CAAATCACATTTTAACAAACCACGTGACCACCTAATATCAGTGTTACAGA
AATCGGGAATCGGACCTAATCAGTTGAGCTACCGATTCAAGGATTAATCA
GAAATCGGGGATTAATCGGAAGGATTAATCGGAGTTAAAATCGGGGGCTT
ATTTTAATATTAATATATTATTTAATATTTAGTTATTATTATATTAGCTA
TTTAGTAACTAATATATTTACTATATTCATAAACTCTATAATTATTCATA
TTTAAAGTAATATAGTATTTTAATTTTAATAATTTATCACATATACTTCG
ATTATGAAATATATATAAGGATTAATCGGATTTCAAAAATCGGATCGGTG
GCTGACCTTACAGGGGATTAATCGGGGATTTATCGGTTAAATCGGGGATT
TTTAGAACACTACCTAATATAGTACTCCCTCTGTCCCATTTTAACTGATG
TTTGACTTTCTAGCACGTATATTGAGATGTAAAAAAATAATATCTACACT
TAATAAAATAATTCAATTCTTATATCAAATAAAAGTTTAGGTTCTAAACT
TTTATTTGATATAAATTTTATAAGAAATTATTATGTTTAGATGTGATTTT
TTTAACATCTTAAAATACGTGTAAAAAAGTCAAAAGCATTTAAAATGGGA
CGGAGGGAGTACTCTATATTACTCCCCCGTCCCCTCATTTGTTTACATTA
GGGACGGGGACTCGGGACGCATTCTAATACTTTCGTAAAACGTAGTTCAA
CAAATATTTTGAAATTTTTTTCTTCTGAATAAAAATTTGATGTTAAAATT
TTTATACAAAAAAGAAAAATTTTAAAAATGAATTATAGAACTATATTTTG
CGTGCGCATTAAAATGCGTGCCGAATTGTGTGAAAAAACTGTAAAGAAAT
GAGAGACAGAGGGAGTAACTATCAGCGTCTTATCCCAGTACATATTAACT
CTCAGTAGAGCCTCCTTTAGCCTGTCCGACTTTAGATCAGAGATTAAAAG
ATTTTAAGTCAATTCATGAAATAACTTAGATTTGAAATTAAAAATAAACC
AGAAATCGATTTCAAACCAATTTAATCAGAAATCATGTTCATCCGGAGAT
TTAATAGCCACAACTTTATGTTCAACTCCCTGGTCCCTCTAGAGACAGAG
AGCGATTCAGCAGCCCTTATCTTCCCAACCATGGGATCAACTCCCTGATC
ATGGATGGGGATGGCTATTTTTGCTGCCCATCTAGCACAAAGTTATTATT
GGAAGATAATTTCTCTTCTGCCTATAACTTTTTCCTGGTAGGGAGAAAAA
ATATTTTATCAGTCTTTGGTTCGTTGATGGCTGATTAAACTCTCCCTGGC
CGGGGCCAACCCCCTTCTGTGAATACGTAGATCTGAGAGTGTCTACAGAT
AAAATCAATTACTAAACAGAATATCTAGAGAAAACAGAGAAAAACGTGAG
GGAGTTGCAAAGCATGGCCCTTCAATATTCAGTCAATCAGTAAGTACTGT
TCTGCCTTTTAGATTCATTTCTCTCATCATGACCTGATGATATAACCATG
CACCAACACACCCTGGGCGACCAGCCAACCACACAATCGAGGATACATGT
CCCTGCACTCTCTTCACCAGTCCACCTGAGCAGGGCCGAAATGGAAGGGA
TATATATTGGGTGGGTGTTTCCGAAACAATCCAAATAACTGCATAATGTA
AATCAACCAAGGTGGCTTACAATTCTAACCCTAATTACTACAATTAACTG
CAACACAAGACATCGCACTGTATATAGCTCCACACCCAGAGGATTATCAG
ATCTGTGAACACATTAAAGAAATAGAACAAGTTTTATCTGGCCAAATGAC
AGACAAAAGAAGGCCAATTACATGCCTAGTGAGATGCGATAAGCTCTTAA
AAAAGGATTAATTAAGTTAAACTAATGGCTCACACTTATTCCTTGTGTCC
TACGGTTTAGGCAAAAATTTCCAACAGGGTGGTGTTCCTATAAAGATCAT
TCTGATTTGCCCCTATCTAGTATCTACAATCATATTTACAGAATCAAATC
ATTCCTTTACTACTTAATATGATCATATTTACCACCCATCTCAAGTCATT
TCTTTACTCCTTAATATGATAGGTTCTTCATTAGTAACAGTACTCTTTGC
ATACGTGAAATCATCCAAATCATTAAGATGTAATCTTTAAAAATCAAATG
TCCAAGTATAGCTGTAATGCCTTTGACATATGAGGCCATTAATATATAAG
ATATCTTAGTTATCTTGTTATTCTACTACTACAGTAGCAGAATGGTTGCC
AACTGACATTGTCTTGTCACTTTAAGGCATTCACCCCCCATCTCAGATTC
TTACTTTATTTCATAGCATGAGATTCTTCGTTTTAACTTTTAAGTATTTG
TAATAGTTATCATATCCAATGCACAAATTATAGCTGATGAGACTAAATGA
TATGAGGTCACTGACCTAGATTATTTTAGTCACCATATTAGTTACCAGAA
TAGTAGCAAAATGGATCGACAGGATAGCATGGTACGGAAACCAGGTATAG
ATCTTATAGCTAAAGGGAGTAAGGTAAAATTGTGAAGAGAGACTGCACAC
ACTGGACAAGGAATTGACACAATGAACTTTTAAGAAGCCTTAAAAGAATA
TATGGGACATAAGTATCGCAGAGGACCCCAAGCATATGTCCCAGAAATTT
CTTAAGAAGATTAATAAGCAAAAATTACGCTCACTTCCAAAGCTCTAACT
TCTCGTCTTGAAGCCCAAGTCTATATCCTTGTCCATCTACATAATCAAAA
GTTCAATTAACTATCAAAATTGGAGTCGATAATCATAGAAAAAACTAGTC
AAGTAGCACATAAGAATTTCAATTTAAAGCTTACAAATTTGGCAACTTCC
TTAGCTTTCTCATCATCAAAACCAAGTGCAGCCATGATCTTGTGACCAGC
TGATTCCTTTTCTGACCACACTCCCAGAAGAAAGTGAGCAGCTGTTACTT
CCCCATCTTCGCCTATAGTTCACATGACATCATAATAAGCACTATCAATA
ACTGATATATCTCAACAAAAAACATGATCACAACTAATTTACTGCAGTTT
GGAATTCCATACTGAGAGCTGAATTTCAATATCAACAGTTCAGGACAGCA
ACCTATACGTCAGCTAGGTTAGCAATTCATCATAAACAGATAAAGATGTG
TTCACATGTGTGATGAGTCTCCTTGAGGAACTTGTTTATAAATTTTAAGT
TCATTCTCCCTTGCTTTCATCAAATTGTCAAGTCATAAAATGACTATTCA
TTTTTCATTTATATTTCAGAAATGACAAACTTTAGTAGCATGTAATTAAC
TTCGTATCCAGCAATATTATATTAGAATAACAATATTATTATTATTAATA
TAATAAATGTAAACTTGGTTGTTACTATTAGGATGGGTACTGTTAAAGTT
GCAACTATTTGACAGTTACCTGCAGTTTTTTAATTAATATATCTGATGGC
CACATAAATTGGACATATAGAAATCACATCATATATATTCTACACACTAA
TTTCTAGCAATTTATGGTAAAACAGATTTACACAAGAATTATATACATTG
CAACAATTAATAGCTTGCAAAACTATGATTGAACTCTTCATGTGTGCTGC
TTCAATACAGGTCCGTTTTATATCTAACCATTACCACTGTATATAATTCT
TGTGTAGATCTGTTTCATACTTTTGTGTTGTTTTACTTTGTTTTGTTTTG
CTTTATTTTACAATTCTCTTTATTCTAGTTATATATCATACTAGCTTATA
ACCCGTGCAATGCACGGGCGGTTAACATATTTGTTATTTTATTATATATA
TTTTAAAGTTACCTAATTTTATTGTAAAAATAAAATTATGATAGAATAAC
ATGGTTTAATAGATTTTTTACTTAACAATTGGATAAATTCTTCATAGTTA
AGTCCGTTAAATGGCTAATTGAAAATTAGGTCGAACATATATATTTTAAT
GAGAATGTTAAAATATTTTCTTTTACATGTTATAATTTAAGGTGGCCTAT
AAACTCAGATATAAACTAACCAATCAACCTTTGATATTTAGTTAATAATA
ATTAATAATATGGTATAAAACATACGCATGAACCAAATACCTCCCCATCA
TACTAACCTAAATTTGATGTTTTGGTTTAATTGATAATAAAAAATATACT
ATAACATGTTATAAATTTAAGAGCTCCGTGGGTCGAATACCAAACGATTC
ACCAAACTCAACTAACTGACCAACCAAGTGAATTTTTATTTTTGGCTTTA
ATAGTGTAATAATATATAGTATATGACAGATTTGTAATATTTCTTTTAAT
ATGAGATCATTAGAGTATTTAAAAATAATGTTATTAATGTATTTTGGTTG
AATAATATCACATGTTATTTATTAATAATTATATTTAATGATATTATATT
TTTATATATATGGCGCCCGTGTTAATTGTAAAAACTCGGTTTTTTTAGTT
AGAAAATCTAAAAAAGATAAAGCGTTTTGATTAAAAAGACAATTACTATG
TTGCTCGATATTATTTTAGAAGATTGATTTTTTTTTATCTAAAAAATATA
TAAAAGATAATTTATTTTAGTTAAAAAGAATAATTGGTTATATATATAGA
TAGGCCCGTACTTCGGCCCAAGTACCGACCGCCCGACCAATATAATAATA
CTCCCTCTATTTTTTATTATTTGACTTTTAGACTTTTGGCACACATCTTT
ACGTATAAATATGTCGCCCATGTCCGTATTAATTGTAAAAGATTAATTTT
TTAGTTGGAAAATTTATAAAAAGATAATATATTTTAGTTAAAAAAGTAAT
TACCATGTTTCTCAATGTTATTTTAGAAGATTGAGTTTTTTAGTCAAAAA
TATAAAAAAGTAATATATTTCAATTAAAAGGATTATCTGGTTATATAGAT
AGGCCCGTACTATGGCCCAAATTAAAAAGAATAATTGGTTATATAGATAG
ACCCAGAATAATTGGTTATATAGATAGGCCCGTATTTTGACCCAAGTACT
GACCTACCAAACTTTTAATGTTTCAGTTTTAAAAGGATTATTATTAATTG
GTTATATAGATAGGCCCGTACTTTGACCCAAATTAAAAAGAATAATTATT
TATATAAATAGGCCCAGAATAATTGGTTATATAGATAGGCCCGTATTTTG
GCCCAAGTACCGACCGACCGACCAAACTTTTAATGTTTCGGCTTTAATAG
TATAGTATAGATATACACAAATTGTTAGTCACACACATATATATTGTACA
CATTAATTCTTAACCTACTCAAGATGGAAAATCAGCATCAGGAACTTTAT
ATCATTTGAATATTTTGGTACGAGGGACCAGGAGTAGTTGTTGCCGCCAT
CTTCAGAATTACAATTGAAATTCGTAAGGGTATAATCCAGGGCGGATACA
ATATATAGGGTAATTCATGTGTTGCGTGCTTAGCTAACATTGCTAGTATA
TTGAAAAACTTAAGTTTGTGATTGTGCCGAGAATCGAGACCTTGATTGCT
TAGTCCTTGTCCCTGAACTAAGGAGTTTGGTTTTCAGCAATTCTATTTGT
AAACAGATAAATTTTTACTTTCACATTTCTGATGAATCTTCAGTAGGAAA
TTGTTAATATATTAAAAATTCTTTCCACATTTCTGTCGGGTTTTCTAGTC
TGTGCCCATGGGCACACACTAACAACCACTTTTTAGCTAGATGGTGCATT
TTGATTGGCTCCCATATCATTAATAATGATGGCCCCCTGCATGCACAAAA
ATCTCCACCAACCAAAATCTTCCACCTATACAAAAAGTAGTTGTTAGTGT
GTGCCCATGGGCACACACTAGAAAGACCCTTCAATTAATTGTCATGTACT
TGTGTCCTAAAATGAGTACTTATTACTGATTATGTTTGAACTTTGAACAA
TGTTAAAATTCAGTGACGTGTCATTGCTTTATATGTAGCAGTATTTCAGA
TGGAAAAATCAACAGCATTAAGCAACAAACTGTATTTCATTTAATCTTAG
TACAAGCGCACAGCTATAGTTGCTGCCGCCATCATTAAAACCACGATTGC
AATATTACCCGATGAGATCTTATAGTTTAGAAGACAGAAAGTCACCTGAC
TTCAGTTTCTCAGCTATAGCCCAATCAAGTGCCCGCTGAGCTGGTTCTGT
GAATGGAGGATGCTCAGGACTGAAAAAGTACATGTCTGATTTTCCAAGCA
ATTTGACAGTCTCATCCCGAACTTTAAAAAGAGTGATACCATTTTCTCTC
AAGAACTTTGCAGCTAAACTGGTTCCTAATATAAAAAAAATAATACATTT
GAATGTGATTTGCATACTATGACATGCGATTTTATCACAAGCCCCTATAT
ACAATAATTATATGTTATTTATTCTGCATAAAATATAACCATTTTTAAGG
TAATATCAGGCTTCTAACAGTGTTAGTTGTCAGATACAAGCAACCACACT
TCCAATCATCGTCAACTAGATTAAGAAAAACTTAACAATGAAATAATCAA
CCGAACAGAAAAGCAACATTATCAAACCTTCGACCAAAATTCCCATGAGA
AGAGCTTCCGTCCCAGTATTAGGATACTTAAGCTTTCTAGCTTCCAATTC
AGCCATTGCATACGACTTTATTGCCCTAGCTGACCATCTTAAACACACAC
ACACACACACACAAGAAAAACATTAATCATCACTATCACCACAAATAGTA
AAACAGAACATTTTACTTAATTATCCCTAGAAACAAGCATTTTAGTTTAG
AATCACTCCTAAACATGATTCTGCAATACGAACAAATATTATTCAATTGA
GAAATTACTTGGGAGACTTCTGCCTCGAGATTCTATCAGGTGTCCTGCAA
TTAAAAATGATGCAATATTAATCCAAAACTGAAATATAAGAATATAAGCA
AGTCAGATTAATTGAATGTATGACACTTTTGAGTACTGCACATAGAAAAT
GAATAGGCAAGCTCAAACTCTTCATGTATAGTCATATCTTTTCAGTTAAG
AAATTAAAAAGACTGGTTGAACTTCTTCACGAGTCATATAGGTTAATAGA
TTAAATAGATAGGTTGAACATTTTTATTTTTTATCTCATGATCCTTATAA
ATAACATGGATTTTGTTCAGTTCACCTGTACTGTAAAAAGTAGTGTGAAA
GAGTAAGTAAAGTGGGTAAAGTGGTGAGATCCATTACTATTTAATTGATA
GATTAAGGAAGATGGATGAAAGTGGTGGGTGAGATTGGTTTTTATATTAT
AAAAGTTTACAATATTTTAAAAGTTTTGAGATGTAAAGAATTGAGAAGCA
CATCCCAAAAAGGAAAGTGTAAAGAAATGAATGGTACAAAAGGAGTATAG
AGTTAGGAGATTATAGTCTTTTAAAAAAATTGACGTTTAGAGGGGATTAT
ATAATACCTCCTCCAACCTAGTCTTAGGGGATTATAATTATATCCAGCTA
TCTAATAAAACAAACATTAAGAGGGCATTGTAATTATGTGCCTGTTTGGG
ACGAGGGGAGAAGCACTTCTTTTCCGAAGAAGTTGGTTTCTGCTTTTCTT
GTCCAGTTTGTGTAAAAAAGCAGAAGCACTTTTTAAAAGTTACAAATGCT
AGCTTTTCTCTCAGAGCTCCTGCTTCTTTCCCAAACACTTTAATCACTTA
TAAGTCCTAATTTGCTTCTAACTTCTACTTCACTTTTTTACTTTAACTAA
GAAGCACTTATTTTAAGCTCATCCAAATGGCCCCTATATCCCGCAATCCA
ACAAAACAAACATGAGATGACATTTTTTTTTTTGCCATAGAGATGACATA
TTTTAAATGACTATAATCTTTAAACCAATCTCAAATGTTTATGCAAAACA
AACATAAAATAAAGAAGTTTACAGGATTATAATCTTATCCCCTGCTTAAT
CCTTTTATGACCCGTGTTATATAAGTTATCAAGTGAAAAGGTATATTTAC
TCCGCTTGACATTTTGCCTATCTACTTCAGTTCTTAACTGAAAACGTACG
ATTACTTAGGAAGAGTTTGAGCCTCTGCTTATTCCACTTCTAAATATCAT
ACTCAAACCGCACAATTATAAAATCCAAGAGTGCATACTTTCAATTCTAG
TCATTACAATTCTAGTTGTTATTTTGTTAATTTCAAATCCACATACATAT
AATCAAGTGAGATAGAAATGAGTAAGAGTTAAGTATGTACCCAGTGGGAA
GACTGACGGAGATGGTGGCCACCGTGGAGGAGTGCTGTGTGTGAGTGTAC
TGCCGCGAATTTGTATAATTAACAGCCAATTTAGCTCCAACGAAAGAGCT
AGCGAGACTAAGATGATAACAGAGTGATGATTTAATTGATTCTTGTTTAC
AGCAAAAATGATTACGAGAAGGTGTCGAAATGGGGAGCAGCGAGAGGGCG
TGAGCAGCCATGGATATTGGATAGAATAGATGGGGAATTGGGTTTCAACT
TTCAAGTATTGAAAGATAAACCCTAATTTTCTAATCTGCCCCTCTTTCTA
ATTTGGTGTAATAACAAGCTTTTGGATGTCAAACACCAGATTCATAATTG
ATTTACAGTAACTTACTAGATTTTACGGTGTAAACAAAGTACAAATCAAC
TTAGATACGCATAAAAGGCCCGGGCCAGTTTCTCATTCCACAATTTTTAG
TTGTAGTTCGGACCGCCCGTGCATAATGGATCGGTTTCGGATTGGATCAA
TTTGAAATTATATTTTTAATTTTATGTTCAGATTTTTTTAATTTAGTCAA
TTCAAATTTGGATTTGATCAGTGTCAGAATTTTTTTAGCATAATTCATAT
TCGGAACTGTCAGAATCATGCTTAAGTAATCAGAATTCCAGATTCGTTTA
AATAATTTAGCATGTTTTGAAACAATATTTCAACTAATTCCAATACATAT
TAAATTACTTAACATAACTTGAAAATGTAAATACAAACACAGTAATAAAT
TCACAAAAAAATGTTAATAATTTTTACATTCTATTTGTAAAAATAAAAAT
TATATAAATAACCAATTAATTTATGTCTAATTTGAACTTAATATTTTTAA
ACTTACTTGATTATGTCCATTAATTTGTTTTAAAAAAGTTAACTCAATGG
TATATAATGAGTTGTTGGCGAGATAAGACATTGATATATTTTGATGTGTT
TAGTGGACAATTTGATATAAAACCGGTAGTTTTTATATGATCCGTTACTT
TAACCAGAATCCTGATTCCTGGGGACTCTGCGGAAATAAATAGCTCACTT
GTTAGTAATCTGCCTTCTCTGGATCCGCCT