DCAR_020544

Resource Type: 
Gene
Name: 
DCAR_020544
Identifier: 
DCAR_020544.gene
Locus Tag: 
DCAR_020544
Publication: 
Iorizzo M, Ellison S, Senalik D, Zeng P, Satapoomin P, Huang J, Bowman M, Iovene M, Sanseverino W, Cavagnaro P, Yildiz M, Macko-Podgórni A, Moranska E, Grzebelus E, Grzebelus D, Ashrafi H, Zheng Z, Cheng S, Spooner D, Van Deynze A, Simon P. A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.. Nature genetics. 2016 06; 48(6):657-66.
Relationship: 
There are 2 relationships.
Relationships
The mRNA, DCAR_020544, is a part of gene, DCAR_020544.
The genetic_marker, MK000039 LCYB, is located in gene, DCAR_020544.
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Analysis: 
NameDescription

An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4.

There are a few different naming schemes for this assembly. First there is the
Authors' original naming scheme: Sequences with DCARv2 prefix are the original assembly as submitted to NCBI. These are labelled DCARv2_Chr1 through DCARv2_Chr9 for the chromosome pseudomolecules, DCARv2_MT and DCARv2_PT for the organellar assemblies, DCARv2_B1 and up for unincorporated superscaffolds, DCARv2_S26.1 and up for unincorporated scaffolds, and DCARv2_C10542132 and up for unincorporated contigs. A file with sequences using this naming scheme can be downloaded from the File: link below.
These sequences can be viewed in JBrowse here.

Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0

LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926.

Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence.

RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records.

The NCBI Sequence report lists the correspondences between the various naming methods

Link to the LNRQ01000000.1 master record at NCBI

Raw Reads: Link to SRA accessions used for the genome assembly

This genome is available in the CarrotOmics Blast Search

For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins.

Data from this analysis can be viewed in JBrowse here.

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Transcripts: 
Transcript NameIdentifierTypeLocation
DCAR_020544DCAR_020544.mRNAmRNADCARv2_Chr6:30446463..30447990
Sequence: 
ATTATTTTCTCGTGGCAACCTAGGCAAAGACATCCCTGCCGCACATGTTT
GGATGATTTGATTTTGGATATCTTCACCCACAAATTATTTTTTATTTTTA
GAAAGAAAATAAAAGAATTTGTTTCAAGTGAGTGCAGCTTACACCTACTT
GATTATATGAAAAATGAGATATGCTTGTATGTATCTCGTCTGTATCTACC
TCCAATCCCTCTTGCTGCTAGATGGGTAAGTCTTTAATTCTTTAACCGTA
GCTTTATTTACTTGTAATTCTCCAATATCTTCTGCAGTTATATGTTTAGT
ACATGTTTTTATTTTGAACAATTTTTTATTAAGTCACTCTGAAATTTCCA
TTAATTATTGGTGAAAGTTAGCATAAAGGTTGAGAATTTGGGATATCTTG
GAACCCAGATTTTGTTTTTGGAGATATATTTGGTAAATTAATAGAACCAT
ACATGAAAGTGATGGATACTCTACTAAAGACTCATAACAAGCTTGAGTTT
TTCAATCCAATTCATGGGTTTCCTGATAAAGTTGGAACCCTGAGTTGTTT
GAAGTTTCGAAACCAGGAGCTGAGGTTTGGCTCTAGAAGATCGAATGTAA
ATTGGGGGAAGAACGGTTCTGTAAAGGCTAGTAGTAGTGCCCTTTTGGAG
CTTGTTCAAGAAACCAAGAAAGAAAATCTTGAATTTGACCTTCCTTTGTA
TGACCCGTCGAATGGCCTTGTAGTGGACTTGGCTGTAGTTGGTGGAGGTC
CTGCAGGGTTAGCGGTAGCACAACAAGTTTCTGAGGCAGGACTTGCGGTT
GTGTCTATAGACCCTTCTCCAAAATTGATATGGCCAAACAATTATGGTGT
TTGGGTGGATGAGTTTGAGGCTATGGATTTGCTAGATTGCCTTGACACTA
CCTGGTCAAGTGCAATTGTTTACATTGATGACCAGACAACCAAAGAGCTC
GGAAGACCGTATGGAAGGGTTAACAGGAAGCAGCTTAAATCTAAAATGAT
GCAGAAGTGCATATCAAATGGAGTTAAATTTCATCAGGCTAAGGTTGTAA
AAGTTGTACACGAGGAAGCTAAATCCCTATTGATATGTAATGATGGTGTG
ACCATTCAAGCTGCTGTAGTTCTTGATGCAACCGGTTTTTCAAGATGTCT
TGTTCAATACGACAAGCCATATAATCCAGGATACCAAGTAGCTTATGGAA
TAGTGGCAGAAGTAGAAGAACATCCTTTTGATGTAAATAAGATGATTTTC
ATGGACTGGAGAGATTCCCATCTTAATGGCAATACAGAATTAAAAGAAAG
AAACAGTAAAATCCCTACGTTTCTTTATGCTATGCCTTTTTCATCTGACA
GAATATTTCTTGAAGAAACATCCCTTGTAGCTCGTCCAGGTTTAGCCATG
GGAGATATTCAGGAGAGAATGGTGGCTCGCCTAAGGCATTTGGGTATTAA
AGTGAAGAGCATTGAAGAGGATGAGAGATGTGTGATCCCAATGGGAGGGC
CTCTGCCTGTACTCCCTCAGAGAGTAGTGGGAATTGGCGGTACAGCTGGT
ATGGTGCATCCTTCCACTGGATATATGGTAGCAAGAACTCTAGCTGCTGC
ACCAGTTGTTGCCAATGCAATAGTTCAGTACCTTGGTGGTTCTAAGAAAG
GCGCTTTGGGAAACGAATTGTCTGCAGAAGTCTGGAAAGATCTATGGCCA
ATAGAGAGAAGACGCCAGCGAGAATTCTTTTGCTTTGGTATGGATATTTT
GCTAAAACTCGATTTGCCTGGTACAAGAAGGTTTTTCAGTGCTTTTTTTG
ACCTGGAACCTCGCTATTGGCATGGATTCTTGTCTTCTCGACTATTTCTT
CCTGAACTATTCTTTTTCGGGCTTTCTCTTTTCTCAAATGCCTCCAACAC
TTCTAGAATAGAGATCATGGCAAAAGGCACGGTTCCTTTGGTAAACATGG
TAAACAATCTGATCAAAGATAGAGAATAGTCATTTTGTGATTTTGACTCA
TTGTGTTATAAATGTTTGTATGTTCTCTGATCATAAATCTAGTTCAAAAT
GCTTGTGAAGTTACACATCTTGGTCGTCCACTTTCTATTCTGTGAACTGT
GAGTTTGCACGTTGCTTACAGGAGATAGGAATTATAAAGTATGTGCAATA
TCATGAGGTAGTAAAAACTGATTGAGAGTTCTCAATTTTTGCTTGGATTT
GTAAATAACTCCTAATTTTTTACGGTATACATATACTACCAGATCGATGC
ATATAAAGTTTAATGTTAGATG
Sequence Length: 
2272
Sequence Checksum: 
b41583d8d6ec9d26588b3cbe5e54f5e4
View location in JBrowse: 
Cds: 
>DCAR_020544cds ATGAAAGTGATGGATACTCTACTAAAGACTCATAACAAGCTTGAGTTTTT CAATCCAATTCATGGGTTTCCTGATAAAGTTGGAACCCTGAGTTGTTTGA AGTTTCGAAACCAGGAGCTGAGGTTTGGCTCTAGAAGATCGAATGTAAAT TGGGGGAAGAACGGTTCTGTAAAGGCTAGTAGTAGTGCCCTTTTGGAGCT TGTTCAAGAAACCAAGAAAGAAAATCTTGAATTTGACCTTCCTTTGTATG ACCCGTCGAATGGCCTTGTAGTGGACTTGGCTGTAGTTGGTGGAGGTCCT GCAGGGTTAGCGGTAGCACAACAAGTTTCTGAGGCAGGACTTGCGGTTGT GTCTATAGACCCTTCTCCAAAATTGATATGGCCAAACAATTATGGTGTTT GGGTGGATGAGTTTGAGGCTATGGATTTGCTAGATTGCCTTGACACTACC TGGTCAAGTGCAATTGTTTACATTGATGACCAGACAACCAAAGAGCTCGG AAGACCGTATGGAAGGGTTAACAGGAAGCAGCTTAAATCTAAAATGATGC AGAAGTGCATATCAAATGGAGTTAAATTTCATCAGGCTAAGGTTGTAAAA GTTGTACACGAGGAAGCTAAATCCCTATTGATATGTAATGATGGTGTGAC CATTCAAGCTGCTGTAGTTCTTGATGCAACCGGTTTTTCAAGATGTCTTG TTCAATACGACAAGCCATATAATCCAGGATACCAAGTAGCTTATGGAATA GTGGCAGAAGTAGAAGAACATCCTTTTGATGTAAATAAGATGATTTTCAT GGACTGGAGAGATTCCCATCTTAATGGCAATACAGAATTAAAAGAAAGAA ACAGTAAAATCCCTACGTTTCTTTATGCTATGCCTTTTTCATCTGACAGA ATATTTCTTGAAGAAACATCCCTTGTAGCTCGTCCAGGTTTAGCCATGGG AGATATTCAGGAGAGAATGGTGGCTCGCCTAAGGCATTTGGGTATTAAAG TGAAGAGCATTGAAGAGGATGAGAGATGTGTGATCCCAATGGGAGGGCCT CTGCCTGTACTCCCTCAGAGAGTAGTGGGAATTGGCGGTACAGCTGGTAT GGTGCATCCTTCCACTGGATATATGGTAGCAAGAACTCTAGCTGCTGCAC CAGTTGTTGCCAATGCAATAGTTCAGTACCTTGGTGGTTCTAAGAAAGGC GCTTTGGGAAACGAATTGTCTGCAGAAGTCTGGAAAGATCTATGGCCAAT AGAGAGAAGACGCCAGCGAGAATTCTTTTGCTTTGGTATGGATATTTTGC TAAAACTCGATTTGCCTGGTACAAGAAGGTTTTTCAGTGCTTTTTTTGAC CTGGAACCTCGCTATTGGCATGGATTCTTGTCTTCTCGACTATTTCTTCC TGAACTATTCTTTTTCGGGCTTTCTCTTTTCTCAAATGCCTCCAACACTT CTAGAATAGAGATCATGGCAAAAGGCACGGTTCCTTTGGTAAACATGGTA AACAATCTGATCAAAGATAGAGAATAG
Pep: 
>DCAR_020544pep MKVMDTLLKTHNKLEFFNPIHGFPDKVGTLSCLKFRNQELRFGSRRSNVN WGKNGSVKASSSALLELVQETKKENLEFDLPLYDPSNGLVVDLAVVGGGP AGLAVAQQVSEAGLAVVSIDPSPKLIWPNNYGVWVDEFEAMDLLDCLDTT WSSAIVYIDDQTTKELGRPYGRVNRKQLKSKMMQKCISNGVKFHQAKVVK VVHEEAKSLLICNDGVTIQAAVVLDATGFSRCLVQYDKPYNPGYQVAYGI VAEVEEHPFDVNKMIFMDWRDSHLNGNTELKERNSKIPTFLYAMPFSSDR IFLEETSLVARPGLAMGDIQERMVARLRHLGIKVKSIEEDERCVIPMGGP LPVLPQRVVGIGGTAGMVHPSTGYMVARTLAAAPVVANAIVQYLGGSKKG ALGNELSAEVWKDLWPIERRRQREFFCFGMDILLKLDLPGTRRFFSAFFD LEPRYWHGFLSSRLFLPELFFFGLSLFSNASNTSRIEIMAKGTVPLVNMV NNLIKDRE*
Gene: 
LCYB1