| Relationships |
|---|
| The mRNA, DCAR_009854, is a part of gene, DCAR_009854. |
| The genetic_marker, MK002705 K0149, is located in gene, DCAR_009854. |
| Name | Description |
|---|---|
An orange, doubled-haploid, Nantes-type carrot (DH1) was used for genome sequencing. We used BAC end sequences and a newly developed linkage map with 2,075 markers to correct 135 scaffolds with one or more chimeric regions. The resulting v2.0 assembly spans 421.5 Mb and contains 4,907 scaffolds (N50 of 12.7 Mb), accounting for ∼90% of the estimated genome size of 473 Mb. The scaftig N50 of 31.2 kb is similar to those of other high-quality genome assemblies such as potato and pepper. About 86% (362 Mb) of the assembled genome is included in only 60 superscaffolds anchored to the nine pseudomolecules. The longest superscaffold spans 30.2 Mb, 85% of chromosome 4. There are a few different naming schemes for this assembly. First there is the Phytozome genome ID 388: The authors' sequences and gene predictions were also submitted to Phytozome, and can be accessed at this address: https://phytozome-next.jgi.doe.gov/info/Dcarota_v2_0 LNRQ01: These sequences were then assigned GenBank accession numbers starting at LNRQ01000001.1 which corresponds to DCARv2_Chr1, up to LNRQ01004826.1 which corresponds to an unincorporated contig, DCARv2_C10750146. These reside in bioproject PRJNA268187, which is a subproject of umbrella project PRJNA285926. Assembly GCA_001625215.1: The genome assembly was later defined an accession number GCA_001625215.1 for assembly ASM162521v1 which consists of only the 9 chromosome sequences and the plastid assembly, which have accession numbers from CM004278.1 to CM004286.1 for the chromosomes and CM004358.1 for the plastid. The mitochondrial genome was not included because it is classified as an incomplete sequence. RefSeq: The assembly was then later added to RefSeq, and there another new set of identifiers was defined from NC_030381.1 to NC_030389.1 for the chromosomes, and from NW_016089425.1 to NW_016094239.1 for unincorporated scaffolds and contigs. These reside in bioproject PRJNA326436. Note that NCBI substituted different assembled organellar genomes from different genotypes for the RefSeq records. The NCBI Sequence report lists the correspondences between the various naming methods Link to the LNRQ01000000.1 master record at NCBI Raw Reads: Link to SRA accessions used for the genome assembly This genome is available in the CarrotOmics Blast Search | |
For gene model prediction, mobile element–related repeats were masked using RepeatMasker. De novo prediction using AUGUSTUS v2.5.5, GENSCAN v.1.1.0, and GlimmerHMM-3.0.1 was trained using model species A. thaliana and S. lycoperisum training sets. The protein sequences of S. lycoperisum, Solanum tuberosum, A. thaliana, Brassica rapa, and Oryza sativa were mapped to the carrot genome using TBLASTN (BLAST All 2.2.23) and analyzed with GeneWise version 2.2.0. Carrot ESTs were aligned to the genome using BLAT and analyzed with PASA to detect spliced gene models. RNA-seq reads from 20 DH1 libraries were aligned with TopHat 2.0.9. Transcripts were predicted by Cufflinks. All gene models produced by de novo prediction, protein homology searches, and prediction and transcript-based evidence were integrated using GLEAN v1.1. Putative gene functions were assigned using the best BLASTP match to SwissProt and TrEMBL databases. Gene motifs and domains were determined with InterProScan version 4.7 against the ProDom, PRINTS, Pfam, SMART, PANTHER, and PROSITE protein databases. GO IDs for each gene were obtained from the corresponding InterPro entries. All genes were aligned against KEGG (release 58) proteins. Data from this analysis can be viewed in JBrowse here. |
| Transcript Name | Identifier | Type | Location |
|---|---|---|---|
| DCAR_009854 | DCAR_009854.mRNA | mRNA | DCARv2_Chr3:11683864..11691796 |
AAACCGTGGCCTGAATCATAAAATCAAAAAAATAAATGCACAAGGCAAAT
AGAGGCCCACGTATCCAAAGAGAAGAGGCAGTTAAGCGAGTGGTCTAGCT
TAGCTTGTGTTGAACTCAAACTCTTTTAAATTATCAATACTCTCAAATTC
GAATGCTTTAACATAATCTCGGCAATGACGATTACAGCTACGGCGGCGGC
GGCGGCAGTAGTTGGCGGAGGATCCCCTGTAATAAACAATCTCCATCATC
GTATTCGTATTGGTATTGAATCGTTAACACCCAGGCTTTCTCGTACTAAT
GGAACCTCCAGCTCTGAGTTGTTCAGTTCTCAGTTGCGGCCGCTTCTGAA
TCATCGTAAAAACAAACTCAATTTCATTTCCCGAGCAGCTGATTCTGCAT
CTACTCAACCTGCTGCTGCACCCAATAAGTCCTCTCTGGTAACCGATGAG
GAATTCTCTCTTGCCAAGGTATTCTAATTTATCCTCACCAAATTCATTTT
GTGTTTTATTCTTTGATTCAAGAAAATTCAGTTTTGTGATTTGTTCTGGT
TCTTGTTTGAAGATGATCTTAGATATGGTTCTGGTTTGTGCATTTTTTAT
TTTTTATTTTTATATTTTCAATATTATGATATGACTATTTATTGAGTTAT
TAGCACTTTACATAATTACATCCCTTATTTTTTATGCTTTTTGGGTTTAG
TCTCGACACATGTTTCTTGTTATATTACACCTCTAAATTTTAAACTTGTT
TAACTTCGCATCTTTTTACCAAATTCCGTCCGAATGACTATTACAGTTAG
CATAAGTTGGGGTAAAATATTAAAATTTTATATAATTATAATTTGAATTT
AAATAAATCTTTCGTATTCTACCTCTTTTCATGCCTAAAATAATAGTTAA
TATGAACGGAATTTAGTCAATAGGGTACAATATGAAGCGAGTTCAAAACA
TGAATGTGTAATATGTAAAGAAATATTGTTTGAGACCAAATCAAAGTACG
CCCAAAAATAAGGTGTGCAAAAATAAGGTGTGCAGAGGTCTTTTTATTTT
CTTTTGACCAAGGAAGAAAGCTTTCATTCAATTAAATATCAGGCATACAA
AGAGATAAGATGTCACCAGGAACATCAAGTTCTGAAAAGCTACGATCCGA
GTGAAGATAAGATTGCCTTGCTAAGCAGTGTGCCACCCTATTTGCTGACC
GTCTAATGAAGCAAACTTCAACATTAACAATGTCTTGCAGGAGTTGTCTG
CAATCCAACACAACCAACCCAAACGGAGAAGATAACTCCACCGAACTACG
CAGAGCTTGCACTACCTTCATACAATCAGATTCCACCAGCACTCTAGTCC
ACTGATTCGTTTTAACCCAACTAAGAGCTTCCTTAACAGCTATCACCTCT
GCTAGATTTGTAGTTATTCTTCCAGTTTTGCTCGCCATCTTGGCTCCCAG
AAATCGCCCGGAATGATCCCGAGCTACACACCCAAACCCAAAGTAATGTT
TATCATTGAAAAGGGCTGCATCAACATTTATCTTGATTGTGTTGACATCT
GGTTTCGCCCAAACCTCACTACCATCCCTCGCATTCAAAGACACAACAGA
CGGAGTAAAATTCCTCAACTGAGCTTACAACCATTGATCAAGAGTAATCT
GAGCAGTGCGAATAACCTCATCAACGTGAAGGTATGTATCGTGCGAAACC
ACTGTGTTACGAGCTTTCCAAACAGACCACAGCAACATCACCAACTTAGC
CAGCCTCAAGTTGTCATACATATTCATTGCGCCTTGCAGCCAATCAACAA
AGTTATCAAAATCATCCGGAGCGTTGCCAAACCCCGCCCCTTGCCTACAA
GCCTCGGCAAAATCGCATTGAATCAAGCAATGTAATGTTGTTTCTCTCTA
TCTAAACACATTGGACATTGCATAAGAATAGGAACATGACTCCTGTTTAA
AGATGATTTTGTCGGTAAAAAACCATTACAAGCCCTCCATAAAAAGTTTT
TAGTTGTAGGTGGGATTTTCAGGTTCCATAAACGTCTCCAGAAGCCAGAA
TTGGCATTAGACACCCACGCTCCTTTCATTTCCTGCACTAGTCTGTATGC
ACTTTTAACAGTATACATACCTTTATCATCTTTAGACCAGTATCTAGTAT
CCTCCTCCTGACTATAACTAAGAGGAATCCCGAAAATCAGATGTTGATCC
CTCTCCACAAATAAATATGAGATGACCTCAGTGTCCCAGGCTTTTCCCCC
CGGTTTCATCAGAGCAGTTACACACTGATCAACAAGAGCTGGGTGATTAC
TAACAAAAAATGGATGGTCAGGATCATTCAACCAAGCATCATTTAGAATA
CTTACCCTTTCCCCAGACCCTACCCTCCACTTTGTACCAGCCTTCACAAG
ACTCTGAGAGGCCCAAATGCTGCAGAGTTCTTATTACTCTTGTTTACTTC
TTACATTTTTCCCGTCAATCCCCTTAATGCATACAGTGTGAGCACAAAAC
AGTAAAACACAACTCAGTCTTAAGTAATTCCTTTTGTTTATTATTTTTCC
CCTCATTTTTCTTACAGTAGTGTAAGTATTTCAAAATATCATGATTAAAT
ATTTTAGAGATATTTTAATAGTGCCTTTATATTTCAACTTCTCCAAGTAG
TTTTCTTTTTATTATGTCTTATGAGTGATACCTTTGTATTTTAAGTTTTA
TCTATATCAATATATCACGAAATATTGTTATATAAATGTTAAATTTTAAG
TAAATGACTTAATTAGTCATTTAAAATTCATAGAGGTTCATCTAAGTTTG
ATTTTTTTTGAGTTTAAAAAACCATTTAGATAAACTTTGAGTGATTAAAA
TAAATCAAATTCGTGTTTGATTTCACTCTATAAACACTATAAGCTAAAAG
GTCGGATCCATTACAAATGGAATTGAATAAACAACACTAATAATACATCT
GGTGTATCATAAATTGTTATTATAATTTGTGCTTGTTTATTGACAGGTTT
CTTTTGGTGTCATTGGCTTATATGGTGGAGTGTCACTTCTTGGGTGAGTA
AATACTTTTGTGACTATACCCCAGGTTCCTCATTCTTAGTTATTTGTGGG
AAATAGAGAGAACACATAGTTTTTGAAAAAATGTAAATTTTATAAAGTCT
ATTACTCCCTCCGTCTCTTAATACTTTTCATGTTTGCCTTTATCACGTTT
GCCAACACAAACTTTTAATCATTAATATCTTTAATTTCATATTAGTATTA
AATATATAAATTTCATCGTATTAAAGTACTCATAAATATGAATCTAACAA
GATCTCTCATGACTATTTGGTCTTATAAATTATGCGTGAATTAGTAATTT
GTCTCATGTTGCGAACAGTTCCAACATCTCAAACAGGAAAAGTAAAAAGA
ACCATAGGGAGTATTATTTGAATATTTGGATATTTGTGCTCACAAAATTC
AATATCATTTCTTTTGTGTTCTTATTAGTTTTTTTTCTTCAAGATAATAA
CAAGATAAATTCAAAATTTAAATGAATGAACTTTTGTGTCCTCTTCCGTA
TGATGATGTGTATTATTATTTCAATAAAATTGACAGATATGGTTTTGGAG
CATACTTTAACATCCTTCCTGGATCTGAATGGTCTGCAATAATGCTAACT
TATGGATTTCCTCTTGCAATTATTGGCATGGCTCTTAAGGTATAATTATA
TAATTTTATTAGTCTTTAAGCAATTAAAGTGTGCTTCATATGCTTTCCTA
ATCAAAATTTTAGTTTTTTTATCTTGGGATTGTTTCTATTATTACATTCC
GTTTTACACACACATGCGCGTATTTTTATATATGTGTGTGTGTGTGTGGG
GGGGGGGGGGGGGGGTGTATATTATTTTTACAATCGTACTCGTGAAATAA
TGCAGTTACTCTTTTTCCTTTCAGTTTAGAACCNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNCAAGTGAAACATTTTCCTATGGAAAAGCATCTTCCTG
GTGAAACCGATGGGAAGATTTGGATGTGGAGTCGCTGTGGTAATTGTAAA
CCTCAAAGTGGCAACACACAATCCACAAAAAGAGTCTTGATATCAGCTGA
TGCCCGTGGGTTGTCATTTGGAAAGTTTCTGGAACTTAGCTTATCGAACC
ACCTGTCGTCTTGTAGTTTCTCTATATGTGGGCATCCATTGCATACTGAC
TGCCTCTACTTCTTTGGGTATGTATATTTGTGTGTTCTTTCGTTTTGCAG
AATGGACTATTTTATAACTGAGTTTTTAAGTTTCTTGTGTGTGGGGGTGT
GGGGGGGGGGGGGGGGGGGGTTGTACTTCTATCTTGGTAAAAGCCACAAT
ACATGCTTTTCATTTAAATCTTTCACTCTTAATAATCATCAATAAATTAA
TATAATCATGTTATTATTTATAAGTTACAAACTCTATTAACACAATAAAT
TTTCTTATTTATAAAATCTAATTACATTATTCATGTTTTAATTAAATTTA
TTATGTTTAACAAAACAAGTCAAATTTAACAATTCTGAACATAGATTTCT
TTTTTACAGTATGCAGAACTTAAACCAGTTCCATGTGTGACTTATGCTGA
TGCTCAAACAATAAGGGAGAAATGCGCCACTCCAATCCTTAAGCAGGTAT
ATATTTTATATAAATATATGTTAACATTGGTATTCATCACAAACCACTCT
TTTCTATTTTGCAAATATTTTTTTGTCACATTCTATTTTGCAATTTTAAA
TACTATATAATATCGAATTGGGTAATAATCAGTTTTGTTGCCTAAAAGCA
ATGACTCGTGAGATATAAAATCTCAACTACATTTGTGGAATACTAGAATG
AATATTTGGTTTTGCTTAGTAGGTCAGGAATGATGTTATCAGATACCGAT
ATGGTGATGAACAACACTTGGAAGAGGCACTTAAACGAATTTTTCAGTAT
GGTTTGGTATGTTGCTCTCACTCCCTTTGTACTTTCACAAGGTTTAATTC
GTTAGGCTTACTTTTGTTGAGTTTACTAATCATTTAATCCATTACTTTTG
TTGAATTTACTAATCATTTATCCAGTTAGTACAAATCTCTCACATTCTCT
TTATAGTAAGAGTTCATTGCATTAATCTTGAAGTGAAAGTGTTCATCTCT
TTGGTTACCCTGAGATCTATTAACAACTATAACATTGTTTTTCTCTTATT
GTGCTTTATGCCTCTTCGCTTGGAATGATTATTATATCTCGGCAATATGG
AATTGAGTAGGTCTTTATTATGTGACAAGAGCACATGTGACGAAAATGAA
AAATAATAGCTTAATACACTAAACGCCTAGTTGAATAGTTATCTCTTCAA
AACGCGAGTGTGTTGCCGCTAGCAGTAATGTGTGGGATCTAGGCAACTTT
GTGTAATTACAATTGATCTAGTCATGTTATAAGTCAAATATTATTACATG
TATTGAGTATAGCATCAACTTAAATATCGATTCATTAATCATGTTAAATA
TCACAATCTTATCCCGATTATAATTTAGCTTTAGTTGTAACATTTATATA
AATTACTACAAATACAATTCTTATAGTGTTTCCCATCTATCGACTAACAC
ACCATAATTGATTCTTTTTTTTAAACCTCTACTTAGTTCATTCCGTTTAA
GCAGTTGATATATGCTTGTACCGGTCATCAAAACTTGCTCTTGGAAAGTG
AATTTGCCTCAAAATTTTATATTTTATGTAAGACCCTTCAACATCCTCGA
CTGACCAATGATAAATTATTAGTTATAGGAGGTGAAATTTTGTATTATGC
TTAAATATTATCTTCAGTCCAACCCTGTTTTTAGATCATTATAGATGCAA
ACATTGATATACCTTTTTGCTGCTCTCATTAATTATTAATAATGTTTAGT
GAAAGTGTGTTATACTTTTTAATACAACATGATCAATGATGTTTCAAATC
AACTCGATTTTTAGATTAATGAGTTTGGAGGTATTTTTGATTGAGTTGAT
TAATATATGTATCGACAGACTCAGAGCAAGTCCAATGCTAAAACTAGAAT
TGATGTAGCTATTGCTATAATATGAAATCAAAAAGTACATCTTGACGCTA
AAATAACTTTCATTCTCCAATGCATGATTTTAAAATAGAACCAAAGAGAT
GTAAAATATGAACACAAGTACAAAAAATTAAATCAAAATTGTTTTTATAT
TCATTCCCTCCAAATTCCCACTTACATTGCCCATGTAGCAATTATAGCTA
CATCTATACTTGGTTCTGTATGTAGAACCAAGGATAGCATTTTTCTATTT
TAGCATCTCATATAGCATTGCATTGGAATGCTAAGTTTTATAATTGATGC
TATATTATAGAAATAGCACCTCCTATAACATTGCATTGGACTTGCTCTCA
TACATTGAGTCAAGTTCCTTCTTAATATGTTTTATATTAACTTCGATGCG
AGTCAATTTTCTTTTACCTCTTTAGGTTGAGTTTTACCTGGATCAAGTTC
GATATGATCATGTTTCATGAATTATGGTTGAATAATATCTTGCTACATAT
TAAATGAGTAAATAATCAACGATAGAGGAAAGGAATCCCTTATATTATCG
CAGTATGAAAATGTGGAAAATCTTTTTTTGTGTCTATTCATGTGTTTAAC
ATTACCGTCTTTTCTTTATATGTTTAGGGTGGAGGGATCGCGCGTAGAAG
TGCTCCAACTTTACAAATGATTCGTGAAGAGGTATGTCTTTATAACATGG
TTTAGTTTTGTGGATCTAAAGTATGTGTACCGAATTTCAAGAATTTATTT
TTATGTGATTTAAAAGTTTTGTTAATATTTCAATGTTGTAAGAATTACAA
AATATTTTTGTAATATTCTACTCTCAGTTGACTGATATTCATGTGAAACT
CGGAATTAAAATTTAAATTGCACCAGGTTTCAAAATACTGAGATCTCTCC
AAAATTCAATATCCTAGGCAAATTGTTAATAATTATAAATCCTTATTTTA
TATATCCGTTTATTAATATTAAACCTTTTTTAATATTTGATTTGATATGA
TATTACCTCGCTACTTGATTATCAAATTGTTAATAATTATCAATCCCTAT
TTTATATATCCGTTTATTAATATTAAACCTTTTTTAATATTTGATTTGAT
ATGATATTACCTCGCTACTTGATTATCTGATTATGTGAAAGAACTTGTTT
CATGGCATATATATGACTTGATTCATCATAATTTTTACCAATATGTTTGG
TACCCCAGGTCACGGAAAATGGTAGATACTGTTTAGTGTTAGTGTTTGAA
GCAAAAGACCTACAATTGTCTGATTTTGAGAAAAGACAGGTAACATACAA
TTTTGATCAATGTTTGCTTATTATTTTCATCGTATGGAGATAGTCTGCTT
ATTTCTTCATGATTTGAAAACTTGTAGATTTATTTTTACATTCTAACATA
GACAATTGTAGAACATAATCAGCATATCAGAAATGTCATTTATGTTGAAA
TGGTTTCATGTGTAGGCAAAGTTTGCTTCTTTCTTTGGACCAGATATATC
AGCTGAAGTTGGTAAGGACTAAACGCGCTTGTTAAGAAACTATTTTTTTT
TAAATGGTCGTTTATGTCAAACGTTCGTCATTTGAATGATACTATACTTC
GTATATATCATAAAAATTACTATTAATATTCAATAACAAATTGAAAACAT
ATAATTTAAAATAAAAGAAAGAGAATGCTCACGCTAATACCTATTACTTA
TTATGTCTTCAAGATACCTTGGGCATAAGAGTAATGTTAGTAGGATAATA
CTATTCAATACATTTTTGTAGAACTAATCCACTAAAGTTTTGAAATGCAG
TTGAGGGAGAAGGGAATTTGTACGAAGTTCGTCTCATCTCTGATACAACT
TTGTAGTCATCTTTGTAGATTGATGCCTAGAGACGCTGCTGATCAGTTCC
AACATCATTTCATGAAAAAAAATTTAGTCAACACAAATCACTTGTAAATT
GTTCTATATATGTACTAGAATGCAATTTGTTTAAAAGTTTTGAACATTAT
TGTTGTATGCTTTAACC